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| Metadata | Values (examples) | ||||||||
| Asset Title | Timecourse data for yeast glycolytic oscillation | ||||||||
| Uploader | Franco du Preez | ||||||||
| Uploader SEEK ID | 355 | ||||||||
| Project | Project | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 220 | ||||||||
| Assay Title | Metabolite concentrations in yeast glycolytic oscillations | ||||||||
| Assay_type | discontinuousEnzymatic | ||||||||
| Technology_type | enzymatic activity measurements | ||||||||
| Description | Cells are sprayed into -40C methanol to halt metabolism, concentration of glycolytic intermediates are then determined by enzymatic assays | ||||||||
| Experimentalist | P Richard and B M Bakker and B Teusink and K Van Dam and H V Westerhoff | ||||||||
| Date | 1996 | ||||||||
| SOP | |||||||||
| Publication (optional) | P Richard and B M Bakker and B Teusink and K Van Dam and H V Westerhoff (1996) European Journal of Biochemistry / FEBS 235 (1) 238-241 | ||||||||
| Experimental_conditions | |||||||||
| Item | temperature | pH | |||||||
| Compound (if concentration) | |||||||||
| Unit | |||||||||
| Start_value (optional) | 25 | 6.8 | |||||||
| End_value (optional) | 25 | 6.8 | |||||||
| Comments | |||||||||
| Culture growth | Batch | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | enzymeActivity | FactorsStudied | |||||||
| Compound (if concentration) | |||||||||
| Unit | |||||||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
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| Sample Name | Treatments (Factors Studied) | ||||||||
| Organism | NCBI_ID | Strain | Genotype | Phenotype | Respiration | concentration | concentration | ||
| sample | Saccharomyces cerevisiae | 4932 | X2180 diploid | semi-aerobic | Glucose 30mM | cyanide 5mM | |||
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| Metabolites | Chebi Identifiers | Units | Measurements | |||||||||||||||||||||||||||||||
| Time | S | 0.5 | 5.5 | 10.5 | 14.5 | 20.5 | 25.5 | 31.000000000000004 | 40.5 | 45.5 | 51 | 55.5 | 60 | 65.5 | 70 | 76 | 80.5 | 85.5 | 90.5 | 95.5 | 100.5 | 105 | 110.5 | 115.5 | ||||||||||
| ADP | CHEBI:16761 | mM | 1.4840425531914894 | 0.6382978723404256 | 0.7021276595744681 | 0.9095744680851063 | 1.0691489361702127 | 2.393617021276596 | 2.297872340425532 | 1.898936170212766 | 1.0691489361702127 | 0.7021276595744681 | 0.6702127659574468 | 0.8457446808510638 | 1.2127659574468086 | 1.9148936170212767 | 2.393617021276596 | 2.2819148936170213 | 1.324468085106383 | 0.6702127659574468 | 1.3882978723404256 | 1.0372340425531914 | 1.3404255319148937 | 1.851063829787234 | 1.8670212765957446 | |||||||||
| ATP | CHEBI:15422 | mM | 2.5925925925925926 | 3.1666666666666665 | 3.2407407407407405 | 3 | 2.2407407407407405 | 2.5370370370370368 | 1.611111111111111 | 2.0925925925925926 | 2.4074074074074074 | 2.648148148148148 | 2.7962962962962963 | 2.462962962962963 | 2.1666666666666665 | 1.7777777777777777 | 1.6666666666666665 | 1.8703703703703702 | 2.314814814814815 | 2.2777777777777777 | 1.574074074074074 | 1.8888888888888888 | 1.2777777777777777 | 1.1296296296296295 | 1.074074074074074 | |||||||||
| D-fructose 1,6-biphosphate | CHEBI:16905 | mM | 7.530201342281879 | 6.046979865771812 | 4.506711409395973 | 3.4798657718120807 | 2.5671140939597317 | 5.3624161073825505 | 5.989932885906041 | 7.587248322147651 | 6.1610738255033555 | 5.134228187919463 | 3.7651006711409396 | 2.8523489932885906 | 2.51006711409396 | 3.5369127516778525 | 6.1610738255033555 | 7.073825503355705 | 5.989932885906041 | 4.3355704697986575 | 2.8523489932885906 | 2.5671140939597317 | 2.5671140939597317 | 3.9932885906040267 | 5.533557046979866 | |||||||||
| D-fructose 6-phosphate | CHEBI:15946 | mM | 0.16778523489932887 | 0.3825503355704698 | 0.5234899328859061 | 0.6510067114093959 | 0.5436241610738255 | 0.5100671140939598 | 0.20134228187919462 | 0.1610738255033557 | 0.2214765100671141 | 0.38926174496644295 | 0.5771812080536913 | 0.6644295302013423 | 0.6644295302013423 | 0.40268456375838924 | 0.18120805369127516 | 0.44966442953020136 | 0.4966442953020134 | 0.6241610738255033 | 0.8590604026845637 | 0.9530201342281879 | 0.6442953020134228 | 0.3825503355704698 | 0.37583892617449666 | |||||||||
| D-glucose 6-phosphate | CHEBI:58225 | mM | 2.0939597315436242 | 3.38255033557047 | 4.724832214765101 | 4.510067114093959 | 5.369127516778524 | 7.4630872483221475 | 3.436241610738255 | 2.3087248322147653 | 2.4161073825503356 | 3.38255033557047 | 4.778523489932886 | 5.476510067114094 | 5.959731543624161 | 5.691275167785235 | 3.436241610738255 | 1.6644295302013423 | 1.8791946308724832 | 3.2751677852348995 | 3.3288590604026846 | 5.369127516778524 | 4.402684563758389 | 4.1879194630872485 | 2.4161073825503356 | |||||||||
| dihydroxyacetone phosphate | CHEBI:16108 | mM | 2.6308724832214767 | 2.5134228187919465 | 2.442953020134228 | 2.5369127516778525 | 2.0906040268456376 | 3.1711409395973154 | 2.4194630872483223 | 2.748322147651007 | 2.5838926174496644 | 2.5369127516778525 | 2.6308724832214767 | 2.466442953020134 | 2.3959731543624163 | 2.5134228187919465 | 2.6543624161073827 | 2.5369127516778525 | 2.4899328859060406 | 2.5604026845637584 | 2.208053691275168 | 2.5134228187919465 | 2.0201342281879193 | 2.302013422818792 | 2.302013422818792 | |||||||||
| AMP | CHEBI:16027 | mM | 0.2127659574468085 | 0.09042553191489362 | 0.05851063829787234 | 0.11170212765957446 | 0.14361702127659576 | 0.44680851063829785 | 0.648936170212766 | 0.4521276595744681 | 0.14893617021276595 | 0.06914893617021277 | 0.05851063829787234 | 0.09574468085106383 | 0.20212765957446807 | 0.4946808510638298 | 0.7446808510638298 | 0.48936170212765956 | 0.20212765957446807 | 0.11170212765957446 | 0.12234042553191489 | 0.18617021276595744 | 0.44680851063829785 | 0.6861702127659575 | 0.7446808510638298 | |||||||||
