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| Item | Value | Unit | |||||||
| Experimentator | Araz Zeyniyev | ||||||||
| Raw data | |||||||||
| Reference | SYSMO-LAB | ||||||||
| Description | In order to construct an in vivo-like buffer for S. pyogenes, the intracellular concentrations of Fe, K, Mg, Mn Na, P and S elements were determined via ICP-AES (inductively coupled plasma atomic emissionspectroscopy) method at the Institute of Land Use, University of Rostock. The samples for the analysis were obtained from a steady state culture grown on CDM-LAB with glucose. | ||||||||
| Aim | in vivo-like buffer for S. pyogenes | ||||||||
| Overexpression strain | |||||||||
| Organism of Origin | |||||||||
| Vessel type | Chemostat | ||||||||
| Stirring rate | 150 | RPM | |||||||
| Temperature | 750 | ml | |||||||
| Volume | 200 | µl | |||||||
| Gas flow | 0 | ml | |||||||
| pH | none | ||||||||
| SOP's used | 1 | ||||||||
| Explanations | Format | Data | |||||||
| TOPIC | |||||||||
| Title | text | ||||||||
| Comments | text | ||||||||
| ASSAY | |||||||||
| Title | text | ICP-AEM | |||||||
| Assay_type | integer | ||||||||
| Comments | |||||||||
| EXPERIMENT | |||||||||
| Experiment_description | text | OD at 600 nm | |||||||
| Experiment_type | text | ||||||||
| Experimentalist | text | Araz Zeyniyev | |||||||
| Begin_date | date | 2011-08-15T0:0:0+0000 | |||||||
| User | integer | Ara Zeyniyev | |||||||
| Group | integer | IMIKRO | |||||||
| SOP | 1 | ||||||||
| Organism of Origin | |||||||||
| Organism | integer | S.pyogenes | |||||||
| NCBI_ID | integer | 294934 | |||||||
| Strain | text | M49 | |||||||
| Genotype | text | wild-type | |||||||
| Phenotype | text | wild-type | |||||||
| Experimental_conditions | |||||||||
| Item | text | temperature | |||||||
| Start_value | float/text | 37 | |||||||
| End_value | float/text | 37 | |||||||
| Unit | text | celsius degrees | |||||||
| Comments | |||||||||
| FACTORS_STUDIED | |||||||||
| Item | text | elements | |||||||
| Start_value | float/text | ||||||||
| End_value | float/text | ||||||||
| Unit | text | mM | |||||||
| Comments | |||||||||
| DATA_CREATED | |||||||||
| Datatype | text | concentration | |||||||
| Creation_date | date | ||||||||
| Status | text | normalised | |||||||
| Person_created | text | Araz Zeyniyev | |||||||
| DATA_FILE | |||||||||
| File_name | text | ||||||||
| File_type | text | xlsx | |||||||
| Storage_location | text | SYSMOSEEK | |||||||
| Storage_date | date | 2012-04-05T0:0:0+0000 | |||||||
| Last_change_date | date | 2012-04-05T0:0:0+0000 | |||||||
| TemplateID | text | ||||||||
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| Identifer | Element | Concentration | |||||||
| unit | 1.0 | mM | |||||||
| Cu | 3.197159044317359 | ||||||||
| Fe | 0.7575295871485345 | ||||||||
| K | 422.2034103240442 | ||||||||
| Mg | 54.98066351013064 | ||||||||
| Mn | 1.7296561184335337 | ||||||||
| Na | 29.9615404338251 | ||||||||
| P | 1410.7263337453098 | ||||||||
| Zn | 0.8652842890568118 | ||||||||
| S | 275.62050326362754 | ||||||||
