The effects of fructose-1,6-bisphosphate, phosphate and ionic strength on enzyme activity differ for the three LDHs from three Lactic acid bacterias.

Selected Cell

Cell:

Value:

Meta data Organism_sample AssayComposition calculatedResults_Effect of FBP calculatedResults_Effect of Pi Result_Effect of ionic strength

Item	Value
Experimentator	Silvio Hering
Raw data
Reference	SYSMO-LAB

Description	Heterologous Expression of LDHs from different lactic acid bacteria in Escherichia coli DH5α. The effects of fructose-1,6-bisphosphate, phosphate and ionic strength on enzyme activity differ for the three LDHs from three Lactic acid bacterias.
Aim	Assessment of kinetic parameters of LDH to include in a catabolic model
Overexpression strain	E.coli DH5α
Organism of Origin	S. pyogenes, E.faecalis, L.plantarum
Vessel type	384 wellplate
Stirring rate	0	RPM
Temperature	30	degree celcius
Volume	60	µl
Gas flow	0	ml
pH	7.0 and 6.0
SOP's used	1

Explanations	Format	Data

TOPIC
Titel	text	The effects of fructose-1,6-bisphosphate, phosphate and ionic strength on enzyme activity differ for the three LDHs from three Lactic acid bacterias.
Comments	text	some comments

ASSAY
Title	text	LDH kinetics
Assay_type	integer	enzymatic assay
Comments		purified enzyme

EXPERIMENT
Experiment_description	text	Measurements at 380 nm-> Decrease of NADH
Experiment_type	text	Concentration series
Experimentalist	text	Silvio Hering

Begin_date	date	2011-10-12T0:0:0+0000
User	integer	Silvio Hering
Group	integer	SYSMOLAB
SOP		1




Organism of Origin
Organism	integer	S.pyogenes	L.plantarum	E.faeclis
NCBI_ID	integer	294934	220668	226185
Strain	text	M49	WCFS1	V583
Genotype	text	wild-type	wild-type	wild-type
Phenotype	text	wild-type	wild-type	wild-type


Experimental_conditions
Item	text	pH	temperature	buffer
Start_value	float/text	7.0	30	1
End_value	float/text	7.0	30	1
Unit	text	dimensionlesss	ºC
Comments

FACTORS_STUDIED
Item	text	0
Start_value	float/text	0
End_value	float/text	0
Unit	text	0
Comments		influence of allosteric regulators

DATA_CREATED
Datatype	text	rates
Creation_date	date	08/2011-10/2011
Status	text	row, normalised, ....
Person_created	text	Silvio Hering

DATA_FILE
File_name	text	regulation_of_LDHs
File_type	text	excel sheet
Storage_location	text	SYSMOSEEK
Storage_date	date	2012-03-30T0:0:0+0000
Last_change_date	date	2012-03-30T0:0:0+0000
TemplateID	text	Enzyme kinetics template

						Treatment 1	Treatment 2 (activator)	Treatment 3 (activator)	Treatment 4 (ionic strength)	Organism/cell	Culture_description
Identifer	Sample	Organism	Strain	Genotype	Strain comment	pH	Fructose-1.6-bisphophate	Pi	NaCl
unit	1.0						mM	mM	mM
	1	L.plantarum	WCFS1	WT	E.coli DH5α IBA2_Lp_ldhL1	7.0	3	0	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 34.21 kDa/ organism of origin: lp_1101/length: 930 bp
	2	L.plantarum	WCFS1	WT	E.coli DH5α IBA2_Lp_ldhL1	7.0	0	0	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 34.21 kDa/ organism of origin: lp_1101/length: 930 bp
	3	L.plantarum	WCFS1	WT	E.coli DH5α IBA2_Lp_ldhL1	6.0	3	0	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 34.21 kDa/ organism of origin: lp_1101/length: 930 bp
	4	L.plantarum	WCFS1	WT	E.coli DH5α IBA2_Lp_ldhL1	6.0	0	0	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 34.21 kDa/ organism of origin: lp_1101/length: 930 bp
	5	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	7.0	3	0	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	6	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	7.0	0	0	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	7	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	6.0	3	0	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	8	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	6.0	0	0	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	9	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	7.0	3	0	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	10	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	7.0	0	0	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	11	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	6.0	3	0	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	12	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	6.0	0	0	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	13	L.plantarum	WCFS1	WT	E.coli DH5α IBA2_Lp_ldhL1	6.0	0	0	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 34.21 kDa/ organism of origin: lp_1101/length: 930 bp
	14	L.plantarum	WCFS1	WT	E.coli DH5α IBA2_Lp_ldhL1	6.0	0	200	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 34.21 kDa/ organism of origin: lp_1101/length: 930 bp
	15	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	6.0	3	0	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	16	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	6.0	3	200	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	17	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	6.0	0	0	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	18	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	6.0	0	50	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	19	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	6.0	0	100	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	20	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	6.0	0	200	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	21	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	7.0	3	0	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	22	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	7.0	3	200	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	23	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	7.0	0	0	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	24	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	7.0	0	50	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	25	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	7.0	0	100	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	26	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	7.0	0	200	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	27	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	6.0	3	0	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	28	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	6.0	3	200	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	29	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	6.0	0	0	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	30	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	6.0	0	50	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	31	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	6.0	0	100	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	32	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	6.0	0	200	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	33	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	7.0	3	0	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	34	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	7.0	3	200	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	35	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	7.0	0	0	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	36	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	7.0	0	50	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	37	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	7.0	0	100	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	38	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	7.0	0	200	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	39	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	6.0	0	0	50	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	40	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	6.0	0	200	50	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	41	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	6.0	0	50	0	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	42	S.pyogenes	M49	WT	E.coli DH5α IBA2_Sp_ldh	6.0	0	50	200	recombinant protein	L-lactate dehydrogenase/ E.C: 1.1.1.27/ MW: 35.28 kDa/ organism of origin: Spy49_0904c/length: 984 bp
	43	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	7.0	0	0	50	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	44	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	7.0	0	200	50	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	45	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	7.0	0	50	0	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp
	46	E.faecalis	V583	WT	E.coli DH5α IBA2_Ef_ldh1	7.0	0	50	200	recombinant protein	L-lactate dehydrogenase I / E.C: 1.1.1.27/ MW: 35.49 kDa/ organism of origin: EF0255/length: 984 bp


Identifer	Sample	Measurement	Organism	Enzyme	pH	Fructose-1.6-bisphosphate	MES/KOH Buffer	MgCl2	Pyruvate	NADH	Pi	NaCl
unit	1.0	1.0	1.0	1.0	1.0	mM	mM	mM	mM	mM	mM	mM
	1	Effect of FBP	L.plantarum WCFS1	L-LDHLI	7.0	3	100	5	50	4	0	0
	2	Effect of FBP	L.plantarum WCFS1	L-LDHLI	7.0	0	100	5	50	4	0	0
	3	Effect of FBP	L.plantarum WCFS1	L-LDHLI	6.0	3	100	5	50	4	0	0
	4	Effect of FBP	L.plantarum WCFS1	L-LDHLI	6.0	0	100	5	50	4	0	0
	5	Effect of FBP	S.pyogenes M49	L-LDH	7.0	3	100	5	50	4	0	0
	6	Effect of FBP	S.pyogenes M49	L-LDH	7.0	0	100	5	50	4	0	0
	7	Effect of FBP	S.pyogenes M49	L-LDH	6.0	3	100	5	50	4	0	0
	8	Effect of FBP	S.pyogenes M49	L-LDH	6.0	0	100	5	50	4	0	0
	9	Effect of FBP	E.faecalis V583	L-LDHL1	7.0	3	100	5	50	4	0	0
	10	Effect of FBP	E.faecalis V583	L-LDHL1	7.0	0	100	5	50	4	0	0
	11	Effect of FBP	E.faecalis V583	L-LDHL1	6.0	3	100	5	50	4	0	0
	12	Effect of FBP	E.faecalis V583	L-LDHL1	6.0	0	100	5	50	4	0	0
	13	Effect of Pi	L.plantarum WCFS1	L-LDHLI	6.0	0	100	5	50	4	0	0
	14	Effect of Pi	L.plantarum WCFS1	L-LDHLI	6.0	0	100	5	50	4	200	0
	15	Effect of Pi	S.pyogenes M49	L-LDH	6.0	3	100	5	50	4	0	0
	16	Effect of Pi	S.pyogenes M49	L-LDH	6.0	3	100	5	50	4	200	0
	17	Effect of Pi	S.pyogenes M49	L-LDH	6.0	0	100	5	50	4	0	0
	18	Effect of Pi	S.pyogenes M49	L-LDH	6.0	0	100	5	50	4	50	0
	19	Effect of Pi	S.pyogenes M49	L-LDH	6.0	0	100	5	50	4	100	0
	20	Effect of Pi	S.pyogenes M49	L-LDH	6.0	0	100	5	50	4	200	0
	21	Effect of Pi	S.pyogenes M49	L-LDH	7.0	3	100	5	50	4	0	0
	22	Effect of Pi	S.pyogenes M49	L-LDH	7.0	3	100	5	50	4	200	0
	23	Effect of Pi	S.pyogenes M49	L-LDH	7.0	0	100	5	50	4	0	0
	24	Effect of Pi	S.pyogenes M49	L-LDH	7.0	0	100	5	50	4	50	0
	25	Effect of Pi	S.pyogenes M49	L-LDH	7.0	0	100	5	50	4	100	0
	26	Effect of Pi	S.pyogenes M49	L-LDH	7.0	0	100	5	50	4	200	0
	27	Effect of Pi	E.faecalis V583	L-LDHL1	6.0	3	100	5	50	4	0	0
	28	Effect of Pi	E.faecalis V583	L-LDHL1	6.0	3	100	5	50	4	200	0
	29	Effect of Pi	E.faecalis V583	L-LDHL1	6.0	0	100	5	50	4	0	0
	30	Effect of Pi	E.faecalis V583	L-LDHL1	6.0	0	100	5	50	4	50	0
	31	Effect of Pi	E.faecalis V583	L-LDHL1	6.0	0	100	5	50	4	100	0
	32	Effect of Pi	E.faecalis V583	L-LDHL1	6.0	0	100	5	50	4	200	0
	33	Effect of Pi	E.faecalis V583	L-LDHL1	7.0	3	100	5	50	4	0	0
	34	Effect of Pi	E.faecalis V583	L-LDHL1	7.0	3	100	5	50	4	200	0
	35	Effect of Pi	E.faecalis V583	L-LDHL1	7.0	0	100	5	50	4	0	0
	36	Effect of Pi	E.faecalis V583	L-LDHL1	7.0	0	100	5	50	4	50	0
	37	Effect of Pi	E.faecalis V583	L-LDHL1	7.0	0	100	5	50	4	100	0
	38	Effect of Pi	E.faecalis V583	L-LDHL1	7.0	0	100	5	50	4	200	0
	39	Effect of ionic strength	S.pyogenes M49	L-LDH	6.0	0	100	5	50	4	50	0
	40	Effect of ionic strength	S.pyogenes M49	L-LDH	6.0	0	100	5	50	4	50	200
	41	Effect of ionic strength	S.pyogenes M49	L-LDH	6.0	0	100	5	50	4	0	50
	42	Effect of ionic strength	S.pyogenes M49	L-LDH	6.0	0	100	5	50	4	200	50
	43	Effect of ionic strength	E.faecalis V583	L-LDHL1	7.0	0	100	5	50	4	50	0
	44	Effect of ionic strength	E.faecalis V583	L-LDHL1	7.0	0	100	5	50	4	50	200
	45	Effect of ionic strength	E.faecalis V583	L-LDHL1	7.0	0	100	5	50	4	0	50
	46	Effect of ionic strength	E.faecalis V583	L-LDHL1	7.0	0	100	5	50	4	200	50

Identifer

Sample

Measurement

Organism

Enzyme

enzyme activity

standard deviation

commentary

unit

1.0

Effect of FBP

L.plantarum WCFS1

L-LDHLI

7.0

103.30

± 6.6

in presence of fructose-1.6-bisphosphate

Effect of FBP

L.plantarum WCFS1

L-LDHLI

7.0

100.00

± 4.6

in absence of fructose-1.6-bisphosphate

Effect of FBP

L.plantarum WCFS1

L-LDHLI

6.0

95.20

± 5.6

in presence of fructose-1.6-bisphosphate

Effect of FBP

L.plantarum WCFS1

L-LDHLI

6.0

100.00

± 7.4

in absence of fructose-1.6-bisphosphate

Effect of FBP

S.pyogenes M49

L-LDH

7.0

100.00

± 8.0

in presence of fructose-1.6-bisphosphate

Effect of FBP

S.pyogenes M49

L-LDH

7.0

0.60

± 0.0

in absence of fructose-1.6-bisphosphate

Effect of FBP

S.pyogenes M49

L-LDH

6.0

100.00

± 9.2

in presence of fructose-1.6-bisphosphate

Effect of FBP

S.pyogenes M49

L-LDH

6.0

1.20

± 0.3

in absence of fructose-1.6-bisphosphate

Effect of FBP

E.faecalis V583

L-LDHL1

7.0

100.00

± 8.7

in presence of fructose-1.6-bisphosphate

Effect of FBP

E.faecalis V583

L-LDHL1

7.0

19.40

± 0.5

in absence of fructose-1.6-bisphosphate

Effect of FBP

E.faecalis V583

L-LDHL1

6.0

100.00

± 2.8

in presence of fructose-1.6-bisphosphate

Effect of FBP

E.faecalis V583

L-LDHL1

6.0

12.70

± 1.1

in absence of fructose-1.6-bisphosphate

Identifer	Sample	Measurement	Organism	Enzyme	pH	enzyme activity	standard deviation	fructose-1.6-bisphosphate	Pi	commentary
unit	1.0	1.0	1.0	1.0	1.0	%	%	mM	mM
	13	Effect of Pi	L.plantarum WCFS1	L-LDHLI	6.0	100.00	± 8.99	0	0	in absence of fructose-1.6-bisphosphate
	14	Effect of Pi	L.plantarum WCFS1	L-LDHLI	6.0	98.97	± 5.85	0	200	in absence of fructose-1.6-bisphosphate

	15	Effect of Pi	S.pyogenes M49	L-LDH	6.0	100.00	± 9.20	3	0	in presence of fructose-1.6-bisphosphate
	16	Effect of Pi	S.pyogenes M49	L-LDH	6.0	99.29	± 7.80	3	200	in presence of fructose-1.6-bisphosphate
	17	Effect of Pi	S.pyogenes M49	L-LDH	6.0	100.00	± 1.25	0	0	in absence of fructose-1.6-bisphosphate
	18	Effect of Pi	S.pyogenes M49	L-LDH	6.0	176.26	± 0.71	0	50	in absence of fructose-1.6-bisphosphate
	19	Effect of Pi	S.pyogenes M49	L-LDH	6.0	213.67	± 8.81	0	100	in absence of fructose-1.6-bisphosphate
	20	Effect of Pi	S.pyogenes M49	L-LDH	6.0	232.37	± 9.16	0	200	in absence of fructose-1.6-bisphosphate
	21	Effect of Pi	S.pyogenes M49	L-LDH	7.0	100.00	± 8.01	3	0	in presence of fructose-1.6-bisphosphate
	22	Effect of Pi	S.pyogenes M49	L-LDH	7.0	96.68	± 9.90	3	200	in presence of fructose-1.6-bisphosphate
	23	Effect of Pi	S.pyogenes M49	L-LDH	7.0	100.00	± 6.25	0	0	in absence of fructose-1.6-bisphosphate
	24	Effect of Pi	S.pyogenes M49	L-LDH	7.0	263.54	± 8.90	0	50	in absence of fructose-1.6-bisphosphate
	25	Effect of Pi	S.pyogenes M49	L-LDH	7.0	432.29	± 10.58	0	100	in absence of fructose-1.6-bisphosphate
	26	Effect of Pi	S.pyogenes M49	L-LDH	7.0	493.75	± 2.90	0	200	in absence of fructose-1.6-bisphosphate

	27	Effect of Pi	E.faecalis V583	L-LDHL1	6.0	100.00	± 2.76	3	0	in presence of fructose-1.6-bisphosphate
	28	Effect of Pi	E.faecalis V583	L-LDHL1	6.0	106.78	± 8.51	3	200	in presence of fructose-1.6-bisphosphate
	29	Effect of Pi	E.faecalis V583	L-LDHL1	6.0	100.00	± 8.99	0	0	in absence of fructose-1.6-bisphosphate
	30	Effect of Pi	E.faecalis V583	L-LDHL1	6.0	99.64	± 10.92	0	50	in absence of fructose-1.6-bisphosphate
	31	Effect of Pi	E.faecalis V583	L-LDHL1	6.0	157.71	± 2.44	0	100	in absence of fructose-1.6-bisphosphate
	32	Effect of Pi	E.faecalis V583	L-LDHL1	6.0	275.14	± 4.57	0	200	in absence of fructose-1.6-bisphosphate
	33	Effect of Pi	E.faecalis V583	L-LDHL1	7.0	100.00	± 8.70	3	0	in presence of fructose-1.6-bisphosphate
	34	Effect of Pi	E.faecalis V583	L-LDHL1	7.0	98.79	± 15.9	3	200	in presence of fructose-1.6-bisphosphate
	35	Effect of Pi	E.faecalis V583	L-LDHL1	7.0	100.00	± 2.64	0	0	in absence of fructose-1.6-bisphosphate
	36	Effect of Pi	E.faecalis V583	L-LDHL1	7.0	92.86	± 9.30	0	50	in absence of fructose-1.6-bisphosphate
	37	Effect of Pi	E.faecalis V583	L-LDHL1	7.0	142.27	± 0.35	0	100	in absence of fructose-1.6-bisphosphate
	38	Effect of Pi	E.faecalis V583	L-LDHL1	7.0	243.21	± 7.30	0	200	in absence of fructose-1.6-bisphosphate

Identifer	Sample	Measurement	Organism	Enzyme	pH	enzyme activity	standard deviation	Nacl	Pi	commentary
unit	1.0	1.0	1.0	1.0	1.0	%	%	mM	mM
	39	Effect of ionic strength	S.pyogenes M49	L-LDH	6.0	100.00	± 10.41	0	50	in absence of fructose-1.6-bisphosphate
	40	Effect of ionic strength	S.pyogenes M49	L-LDH	6.0	95.35	± 7.64	200	50	in absence of fructose-1.6-bisphosphate
	41	Effect of ionic strength	S.pyogenes M49	L-LDH	6.0	100.00	± 6.74	50	0	in absence of fructose-1.6-bisphosphate
	42	Effect of ionic strength	S.pyogenes M49	L-LDH	6.0	95.35	± 6.87	50	200	in absence of fructose-1.6-bisphosphate
	43	Effect of ionic strength	E.faecalis V583	L-LDHL1	7.0	100.00	± 18.42	0	50	in absence of fructose-1.6-bisphosphate
	44	Effect of ionic strength	E.faecalis V583	L-LDHL1	7.0	100.32	± 7.15	200	50	in absence of fructose-1.6-bisphosphate
	45	Effect of ionic strength	E.faecalis V583	L-LDHL1	7.0	100.00	± 0.43	50	0	in absence of fructose-1.6-bisphosphate
	46	Effect of ionic strength	E.faecalis V583	L-LDHL1	7.0	174.88	± 2.71	50	200	in absence of fructose-1.6-bisphosphate