literature values for allosteric regulation of pyruvate kinase

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final Tabelle3

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#	PMID	PYK	variant/ mutant/ tissue	Reference	conc PEP	Km(PEP) oder Vmax(mM NADH/min) oder Shalf oder nHill	no sugar			fructose-1,6-bisphosphate (FBP)			fructose-2,6-bisphospahte			glucose-6-phosphate			glucose-1-phosphate			glucose-1,6-phosphate			fructose-6-phosphate			fructose-1-phosphate			ribose-bisphosphate			ribose-5-phosphate			Ala			ATP			AMP			ADP			others	pH	temp	buffer	comment	ranking von Km(PEP) oder von Vmax(mM NADH/min) oder Shalf oder nHILL	was verglichen:
							no			F16P			F26P			G6P			G1P			G16P			F6P			F1P			RBP			R5P			Ala			ATP			AMP			ADP
		Organism	PYK				start	end	dev	start	end	dev	start	end	dev	start	end	dev	start	end	dev	start	end	dev	start	end	dev	start	end	dev	start	end	dev	start	end	dev	start	end	dev	start	end	dev	start	end	dev	start	end	dev	others
1	20856875	Toxoplasma gondii	PK1	Bakszt 2010	1 mM	Km(PEP)	0.75			0.55						0.12			0.47			0.47			0.75			0.58			0.5			0.5																			Enzyme is allosterically activated by G6P, which enhance ist acitvity under PEP unsaturated cond.	G6P < G1P < RBP < F16P < F6P < F1P < no
2	20856875	Toxoplasma gondii		Bakszt 2010		Vmax(mM NADP/min)	0.042			0.055						0.044			0.029			0.029			0.044			0.028			0.038			0.038																				F16P > G6P = F6P > no > G1P > RBP > F1P
3	20856875	Toxoplasma gondii		Bakszt 2010		nH	1.71		0.17	1.69		0.19				1.45		0.11	1.61		0.28	1.61		0.28	1.41		0.22	1.56		0.3	1.67		0.23	1.67		0.23																		no > F16P > RBP > G1P > F1P > G6P > F6P
4	12632162	Toxoplasma gondii	tachyzoite	Maeda 2003		Km	1		0.004	0.98		0	1.09		0.02	0.06		0.03	1.2		0.15	1.2		0.15	1,05		0.23				1.12		0.03	1.12		0.03																	no FBP activation	G1P=G16P>RBP=R5P>F26P>F6P>no>F16P>>G6P
5	12632162	Toxoplasma gondii		Maeda 2003		Vmax %	100			102			103			119			115			115			103						95			95
6	12632162	Toxoplasma gondii		Maeda 2003		nH	2.3		0.19	2.21		0.38	2.38		0.08	1.3		0.22	2.43		0.36	2.43		0.36	1.92		0.19				1.97		0.32	1.97		0.32																		G1P=G16P>F26P>no>F16P>RBP=R5P>F6P>G6P
7	2758802	Columba livia	Pigeon erythrocyte	Calomenopoulou 1989	2 mM	Km	0.46			0.15																																								7.4		100 mM Tris/chloride, pH 7.4, 2 mM PEP, 1 mM ADP, 80mM KCI, 10mM MgC12, 0.17 mM NADH, 2 units LDH		no>FBP
8	8074528	Corynebacterium glutamicum	WT			Shalf	1.2																																	0.4			0.4			0.4			2mM ADP	7.7	22	1 mM ADP, 80mM KCI, 10mM MgC12, 0.17 mM NADH
9	8074528	Corynebacterium glutamicum	WT			Shalf	2.8																																										2mM ADP	7.7	22	and 2 units lactate dehydrogenase
10	10751408	Escherichia coli	wildtype			Shalf	3.63			0.08																																								7.5	25	10 mM HEPES, 10 mM MgCl2, 50 mM KCl, 0.12 mM NADH, 22 U/ml Lactate dehydrogenase		no>FBP	Shalf
11	10751408	Escherichia coli	mutant R271L			Shalf	5.21			0.12																																								7.5	25	10 mM HEPES, 10 mM MgCl2, 50 mM KCl, 0.12 mM NADH, 22 U/ml Lactate dehydrogenase		no>FBP	Shalf
12	10751408	Escherichia coli	mutant K382Q			Shalf	7.36			1.1																																								7.5	25	10 mM HEPES, 10 mM MgCl2, 50 mM KCl, 0.12 mM NADH, 22 U/ml Lactate dehydrogenase		no>FBP	Shalf
13	10751408	Escherichia coli	mutant K413Q			Shalf	2.01			0.15																																								7.5	25	10 mM HEPES, 10 mM MgCl2, 50 mM KCl, 0.12 mM NADH, 22 U/ml Lactate dehydrogenase		no>FBP	Shalf
14	10751408	Escherichia coli	mutant R431E			Shalf	1.9			0.05																																								7.5	25	11 mM HEPES, 10 mM MgCl2, 50 mM KCl, 0.12 mM NADH, 22 U/ml Lactate dehydrogenase		no>FBP	Shalf
15	2202599	Escherichia coli	J3/R.387	Speranza_EurJBiochem_vol191_pp701-4_1990	kA	K0,5[mM]	4.3			0.08			1,59																		1,08																		5-phosphorylribose 1-pyrophosphate[5PR1P]:1,.7	7.5	25	10mM Hepes, 10mM MgCl2, 50mM KCl, 2mM ADP, 0.12 mM NADH, 44 U/ml LDH, 0.5 µg pyruvate kinase	The allosteric properties of the fructose-I ,6-bis-phosphate-activated pyruvate kinase from Escherichia coli were examined + - FBP ...as well as of increased ionic strength (NaCl) and of the hydrogen-bond-breaking agent, formamide.	no>F26P>RBP>5PR1P>FBP	K0,5	NaCl und Formamideinfluss nicht eingetragen
16	2202599	Escherichia coli	J3/R.387	Speranza_EurJBiochem_vol191_pp701-4_1990	kA	nHill	3.15			1.05			1,88																		1,75																		5-phosphorylribose 1-pyrophosphate[5PR1P]:2.07	7.5	25	10mM Hepes, 10mM MgCl2, 50mM KCl, 2mM ADP, 0.12 mM NADH, 44 U/ml LDH, 0.5 µg pyruvate kinase	The allosteric properties of the fructose-I ,6-bis-phosphate-activated pyruvate kinase from Escherichia coli were examined + - FBP ...as well as of increased ionic strength (NaCl) and of the hydrogen-bond-breaking agent, formamide.	no>5PR1P>F26P>RBP>FBP	nHill	NaCl und Formamideinfluss nicht eingetragen
17	4258865	Homo sapiens	wildtype		0.1	Km	0.15																																										1 mM Mg2+	8	23	50 mM Tris chloride, 0.25 mM NADP+, 0.1 mg lactate dehydrogenase
18	6250830	Homo sapiens	R-type, wildtype dephosphorylated	Marie 1980		Km	0.6	0.76		0.08	0.14																										1.1	1.7		1.5	1.6									7.3	37	50 mM Tris/HCl, 100 mM KCl, 0.5 mM MgCl2, 0.2 mM NADH, 1 U/ml Lactate dehydrogenase		ATP>Ala>no>FBP	Km
19	6250830	Homo sapiens	R-type, wildtype, phosphorylated	Marie 1980		Km	1.1	1.51		0.09	0.18																										2.2	3.3		2.2	2.3									7.3	37	51 mM Tris/HCl, 100 mM KCl, 0.5 mM MgCl2, 0.2 mM NADH, 1 U/ml Lactate dehydrogenase		Ala=ATP>no>FBP	Km
20	6250830	Homo sapiens	L-type, wildtype, dephosphorylated	Marie 1980		Km	0.4			0.04																											1.2			1.05										7.3	37	50 mM Tris/HCl, 100 mM KCl, 0.5 mM MgCl2, 0.2 mM NADH, 1 U/ml Lactate dehydrogenase		Ala>ATP>no>FBP	Km
21	6250830	Homo sapiens	L-type, wildtype phosphorylated	Marie 1980		Km	0.85			0.06																											2.6			1.5										7.3	37	51 mM Tris/HCl, 100 mM KCl, 0.5 mM MgCl2, 0.2 mM NADH, 1 U/ml Lactate dehydrogenase		Ala>ATP>no>FBP	Km
22	6250830	Homo sapiens	R-type, wildtype dephosphorylated	Marie 1980		nHill	1	1.4		0.8	1.05																										1.5	2.2		1.4	1.5									7.3	37	50 mM Tris/HCl, 100 mM KCl, 0.5 mM MgCl2, 0.2 mM NADH, 1 U/ml Lactate dehydrogenase		Ala>ATP>no>FBP	nHill
23	6250830	Homo sapiens	R-type, wildtype, phosphorylated	Marie 1980		nHill	1.5	2		0.8	1.1																										1.5	3.3		2	3.3									7.3	37	51 mM Tris/HCl, 100 mM KCl, 0.5 mM MgCl2, 0.2 mM NADH, 1 U/ml Lactate dehydrogenase		ATP>Ala=no>FBP	nHill
24	6250830	Homo sapiens	L-type, wildtype, dephosphorylated	Marie 1980		nHill	1.7			1																											1.7			2.2										7.3	37	50 mM Tris/HCl, 100 mM KCl, 0.5 mM MgCl2, 0.2 mM NADH, 1 U/ml Lactate dehydrogenase		ATP>Ala=no>FBP	nHill
25	6250830	Homo sapiens	L-type, wildtype phosphorylated	Marie 1980		nHill	2.2			1																											2.2			2.9										7.3	37	51 mM Tris/HCl, 100 mM KCl, 0.5 mM MgCl2, 0.2 mM NADH, 1 U/ml Lactate dehydrogenase		ATP>Ala=no>FBP	nHill
26	11698298	Homo sapiens	wildtype	Wang_BloodJ_vol98_pp3113-20_2001	var	kcat	349		7	350		10																																						8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 1.5 mM ATP, 0.5-1 µg enzyme; +- 30µg FBP	presence of activator in the kinetics toward PEP shifted the sigmoidal curve of wild-type to a hyperbole	no=FBP	kcat, PEP
27	11698298	Homo sapiens	wildtype	Wang_BloodJ_vol98_pp3113-20_2001	var	Shalf	1.96		0.15	0.42		0.02																																						8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 1.5 mM ATP, 0.5-1 µg enzyme; +- 30µg FBP	presence of activator in the kinetics toward PEP shifted the sigmoidal curve of wild-type to a hyperbole	no>FBP	Shalf, PEP
28	11698298	Homo sapiens	wildtype	Wang_BloodJ_vol98_pp3113-20_2001	var	nHill	2.09		0.11	1.17		0.11																																						8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 1.5 mM ATP, 0.5-1 µg enzyme; +- 30µg FBP	presence of activator in the kinetics toward PEP shifted the sigmoidal curve of wild-type to a hyperbole	no>FBP	nHill, PEP
29	11698298	Homo sapiens	wildtype	Wang_BloodJ_vol98_pp3113-20_2001	0,1 mM	kcat				64.9		5																												64.9		5								8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, +- 1.5 mM ATP, 0.5-1 µg enzyme, 30µg FBP		noATP=ATP	kcat, FBP
30	11698298	Homo sapiens	wildtype	Wang_BloodJ_vol98_pp3113-20_2001	0,1 mM	Shalf				3.4		0.02																												6.7		0.02								8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, +- 1.5 mM ATP, 0.5-1 µg enzyme, 30µg FBP		ATP>noATP	Shalf, FBP
31	11698298	Homo sapiens	wildtype	Wang_BloodJ_vol98_pp3113-20_2001	0,1 mM	nHill				1.56		0.09																												1.81		0.09								8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, +- 1.5 mM ATP, 0.5-1 µg enzyme, 30µg FBP		ATP>noATP	nHill, FBP
32	11698298	Homo sapiens	mutant R510Q	Wang_BloodJ_vol98_pp3113-20_2001	var	kcat	330		8	305		10																																						8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 1.5 mM ATP, 0.5-1 µg enzyme; +- 30µg FBP	presence of activator in the kinetics toward PEP shifted the sigmoidal curve of wild-type to a hyperbole	no>FBP	kcat, PEP
33	11698298	Homo sapiens	mutant R510Q	Wang_BloodJ_vol98_pp3113-20_2001	var	Shalf	3.92		0.35	0.95		0.05																																						8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 1.5 mM ATP, 0.5-1 µg enzyme; +- 30µg FBP	presence of activator in the kinetics toward PEP shifted the sigmoidal curve of wild-type to a hyperbole	no>FBP	Shalf, PEP
34	11698298	Homo sapiens	mutant R510Q	Wang_BloodJ_vol98_pp3113-20_2001	var	nHill	1.99		0.14	2.02		0.13																																						8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 1.5 mM ATP, 0.5-1 µg enzyme; +- 30µg FBP	presence of activator in the kinetics toward PEP shifted the sigmoidal curve of wild-type to a hyperbole	FBP>no	nHill, PEP
35	11698298	Homo sapiens	mutant R510Q	Wang_BloodJ_vol98_pp3113-20_2001	0,1 mM	kcat				35.9		6																												14.4		6								8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, +- 1.5 mM ATP, 0.5-1 µg enzyme, 30µg FBP		noATP>ATP	kcat, FBP
36	11698298	Homo sapiens	mutant R510Q	Wang_BloodJ_vol98_pp3113-20_2001	0,1 mM	Shalf				0.5		0.01																												0.8		0.01								8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, +- 1.5 mM ATP, 0.5-1 µg enzyme, 30µg FBP		ATP>noATP	Shalf, FBP
37	11698298	Homo sapiens	mutant R510Q	Wang_BloodJ_vol98_pp3113-20_2001	0,1 mM	nHill				1.01		0.11																												1.1		0.11								8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, +- 1.5 mM ATP, 0.5-1 µg enzyme, 30µg FBP		ATP>=noATP	nHill, FBP
38	11698298	Homo sapiens	wildtype	Wang_BloodJ_vol98_pp3113-20_2001	var	kcat	355		12	355		11																																						8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 0.5-1 µg enzyme; +- 1 mM FBP		no=FBP	kcat, PEP
39	11698298	Homo sapiens	wildtype	Wang_BloodJ_vol98_pp3113-20_2001	var	Shalf	1.1		0.04	0.18		0.02																																						8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 0.5-1 µg enzyme; +- 1 mM FBP		no>FBP	Shalf, PEP
40	11698298	Homo sapiens	wildtype	Wang_BloodJ_vol98_pp3113-20_2001	var	nHill	1.6		0.16	1.05		0.07																																						8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 0.5-1 µg enzyme; +- 1 mM FBP		no>FBP	nHill, PEP
41	11698298	Homo sapiens	wildtype	Wang_BloodJ_vol98_pp3113-20_2001	5 mM	kcat																																								355		13		8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 0.5-1 µg enzyme			kcat, ADP
42	11698298	Homo sapiens	wildtype	Wang_BloodJ_vol98_pp3113-20_2001	5 mM	Km																																								0.17		0.01		8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 0.5-1 µg enzyme			Km, ADP
43	11698298	Homo sapiens	mutant R510Q	Wang_BloodJ_vol98_pp3113-20_2001	var	kcat	340		10	304		12																																						8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 0.5-1 µg enzyme; +- 1 mM FBP		no>FBP	kcat, PEP
44	11698298	Homo sapiens	mutant R510Q	Wang_BloodJ_vol98_pp3113-20_2001	var	Shalf	1.6		0.11	0.31		0.02																																						8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 0.5-1 µg enzyme; +- 1 mM FBP		no>FBP	Shalf, PEP
45	11698298	Homo sapiens	mutant R510Q	Wang_BloodJ_vol98_pp3113-20_2001	var	nHill	1.75		0.19	1.08		0.08																																						8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 0.5-1 µg enzyme; +- 1 mM FBP		no>FBP	nHill, PEP
46	11698298	Homo sapiens	mutant R510Q	Wang_BloodJ_vol98_pp3113-20_2001	5 mM	kcat																																								351		10		8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 0.5-1 µg enzyme		WT>=mutant	kcat, ADP
47	11698298	Homo sapiens	mutant R510Q	Wang_BloodJ_vol98_pp3113-20_2001	5 mM	Km																																								0.24		0.02		8	37	100 mM Tris, 100 mM KCl, 10 mM MgCl2, 0.5mM EDTA, 0.2mM NADH, 10µg LDH, 1.5mM ADP, 0.5-1 µg enzyme		mutant>WT	Km, ADP
48	3467680	Sus scrofa	dental pulp	ArchsoralBiol_1986_31_559_pigPYK_Kobayashi	0,1 - 2,0 mM	Km	0.053																																							0.286				7.2		50 mM Tris-HCl, 100mM KCl, 10mM MgCl2, 0.15 mM NADH and 10 µg/ml LDH, c[ADP]=0.05 - 1.0 mM		ADP>PEP	Km
49	3467680	Sus scrofa	dental pulp	ArchsoralBiol_1986_31_559_pigPYK_Kobayashi	0,1 - 2,0 mM	nHill	1																																											7.2		50 mM Tris-HCl, 100mM KCl, 10mM MgCl2, 0.15 mM NADH and 10 µg/ml LDH		pH 8,0 > pH 7,2	nHill
50	3467680	Sus scrofa	dental pulp	ArchsoralBiol_1986_31_559_pigPYK_Kobayashi	0,1 - 2,0 mM	nHill	1.2																																											8		50 mM Tris-HCl, 100mM KCl, 10mM MgCl2, 0.15 mM NADH and 10 µg/ml LDH		pH 8,0 > pH 7,3	nHill
51	4441489	rat	Wistar/ liver	vanBerkel_BiochBiophysActa_vol370_pp450-58_1974	0,1 mM	nHill				2.6						2.7						2.7																												7.5	22	25 mM Tris-HCl, 0.1 mM KCI, 1 mM ADP, 0.12 mM NADH, 0.1 mg lactate dehydrogenase,20 mM MgCl2, 5 mM GSH	GIc-1-P, Fru-1-P, Fru-6-P, and Gal-1-P did not influence the pyruvate kinase actvity	G16P=G6P>FBP	nHill
52	4441489	rat	Wistar/ liver	vanBerkel_BiochBiophysActa_vol370_pp450-58_1974	0,25 mM	nHill				2.1						2.1						2																												7.5	22	25 mM Tris-HCl, 0.1 mM KCI, 1 mM ADP, 0.12 mM NADH, 0.1 mg lactate dehydrogenase,20 mM MgCl2, 5 mM GSH	GIc-1-P, Fru-1-P, Fru-6-P, and Gal-1-P did not influence the pyruvate kinase actvity	G6P=FBP>G16P	nHill
53	4441489	rat	Wistar/ liver	vanBerkel_BiochBiophysActa_vol370_pp450-58_1974	0,50 mM	nHill				1.1						1.5						1.6																											Pi: 3.2	7.5	22	25 mM Tris-HCl, 0.1 mM KCI, 1 mM ADP, 0.12 mM NADH, 0.1 mg lactate dehydrogenase,20 mM MgCl2, 5 mM GSH	GIc-1-P, Fru-1-P, Fru-6-P, and Gal-1-P did not influence the pyruvate kinase actvity	Pi>G16P>G6P>FBP	nHill
54	4441489	rat	Wistar/ liver	vanBerkel_BiochBiophysActa_vol370_pp450-58_1974	0,1 mM	nHill+Ala				2.6						2.4						3.3																												7.5	22	25 mM Tris-HCl, 0.1 mM KCI, 1 mM ADP, 0.12 mM NADH, 0.1 mg lactate dehydrogenase,20 mM MgCl2, 5 mM GSH, 1 mM Ala	GIc-1-P, Fru-1-P, Fru-6-P, and Gal-1-P did not influence the pyruvate kinase actvity	G16P>FBP>G6P	nHill+Ala
55	4441489	rat	Wistar/ liver	vanBerkel_BiochBiophysActa_vol370_pp450-58_1974	0,25 mM	nHill+Ala				2.9						2.9						2.9																												7.5	22	25 mM Tris-HCl, 0.1 mM KCI, 1 mM ADP, 0.12 mM NADH, 0.1 mg lactate dehydrogenase,20 mM MgCl2, 5 mM GSH, 1 mM Ala	GIc-1-P, Fru-1-P, Fru-6-P, and Gal-1-P did not influence the pyruvate kinase actvity	G6P=FBP=G16P	nHill+Ala
56	4441489	rat	Wistar/ liver	vanBerkel_BiochBiophysActa_vol370_pp450-58_1974	0,50 mM	nHill+Ala				1.9						1.9						2.2																											Pi: 3.2	7.5	22	25 mM Tris-HCl, 0.1 mM KCI, 1 mM ADP, 0.12 mM NADH, 0.1 mg lactate dehydrogenase,20 mM MgCl2, 5 mM GSH, 1 mM Ala	GIc-1-P, Fru-1-P, Fru-6-P, and Gal-1-P did not influence the pyruvate kinase actvity	G16P>FBP=G6P	nHill+Ala
57	4441489	rat	Wistar/ liver	vanBerkel_BiochBiophysActa_vol370_pp450-58_1974	0,1 mM	nHill+ATP				2.8						1.9						2																												7.5	22	25 mM Tris-HCl, 0.1 mM KCI, 1 mM ADP, 0.12 mM NADH, 0.1 mg lactate dehydrogenase,20 mM MgCl2, 5 mM GSH, 2 mM ATP	GIc-1-P, Fru-1-P, Fru-6-P, and Gal-1-P did not influence the pyruvate kinase actvity	FBP>G16P>G6P	nHill+ATP
58	4441489	rat	Wistar/ liver	vanBerkel_BiochBiophysActa_vol370_pp450-58_1974	0,25 mM	nHill+ATP				2.8						2.2						2.1																												7.5	22	25 mM Tris-HCl, 0.1 mM KCI, 1 mM ADP, 0.12 mM NADH, 0.1 mg lactate dehydrogenase,20 mM MgCl2, 5 mM GSH, 2 mM ATP	GIc-1-P, Fru-1-P, Fru-6-P, and Gal-1-P did not influence the pyruvate kinase actvity	FBP>G6P>G16P	nHill+ATP
59	4441489	rat	Wistar/ liver	vanBerkel_BiochBiophysActa_vol370_pp450-58_1974	0,50 mM	nHill+ATP				2.3						2						2.1																											Pi: 3.2	7.5	22	25 mM Tris-HCl, 0.1 mM KCI, 1 mM ADP, 0.12 mM NADH, 0.1 mg lactate dehydrogenase,20 mM MgCl2, 5 mM GSH, 2 mM ATP	GIc-1-P, Fru-1-P, Fru-6-P, and Gal-1-P did not influence the pyruvate kinase actvity	FBP>G16P>G6P	nHill+ATP
60	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	nHill	1.3			1.8																																							3 mM MnCl2	7.5		80 mM triethanolamine/HCl, 3.3 mM ADP, +- 1 mM Fru-l,6-P 2, 13.3 mM KCI, 0.167 mM NADH, 20 U lactate dehydrogenase, 3 µg protein		FBP+Mn2+>no+Mn2+>FBP+Mg2+	nHill
61	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	nHill				1.2																																							3 mM MgCl2	7.5		80 mM triethanolamine/HCl, 3.3 mM ADP, +- 1 mM Fru-l,6-P 2, 13.3 mM KCI, 0.167 mM NADH, 20 U lactate dehydrogenase, 3 µg protein		FBP+Mn2+>no+Mn2+>FBP+Mg2+
62	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	PEP0,5v	0.09			0.015																																							3 mM MnCl2	7.5		80 mM triethanolamine/HCl, 3.3 mM ADP, +- 1 mM Fru-l,6-P 2, 13.3 mM KCI, 0.167 mM NADH, 20 U lactate dehydrogenase, 3 µg protein		FBP+Mg2+>no+Mn2+>FBP+Mn2+	PEP0,5v
63	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	PEP0,5v				0.18																																							3 mM MgCl2	7.5		80 mM triethanolamine/HCl, 3.3 mM ADP, +- 1 mM Fru-l,6-P 2, 13.3 mM KCI, 0.167 mM NADH, 20 U lactate dehydrogenase, 3 µg protein		FBP+Mg2+>no+Mn2+>FBP+Mn2+
64	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	Km				0.4																																							3 mM MgCl2	6.4		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase		+Mg>+Mn	Km
65	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	Km				0.02																																							3 mM MnCl2	6.4		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase		+Mg>+Mn	Km
66	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	Km				0.18																																							3 mM MgCl2	7.5		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase		+Mg>+Mn	Km
67	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	Km				0.02																																							3 mM MnCl2	7.5		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase		+Mg>+Mn	Km
68	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	Km				0.13																																							3 mM MgCl2	8.75		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase		+Mg=+Mn	Km
69	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	Km				0.13																																							3 mM MnCl2	8.75		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase		+Mg=+Mn	Km
70	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	nHill				1																																							3 mM MgCl2	6.4		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase		+Mn>+Mg	nHill (for +Mg pH independent)
71	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	nHill				1.7																																							3 mM MnCl2	6.4		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase		+Mn>+Mg	nHill (for +Mg pH independent)
72	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	nHill				1																																							3 mM MgCl2	7.5		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase		+Mn>+Mg	nHill (for +Mg pH independent)
73	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	nHill				1.8																																							3 mM MnCl2	7.5		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase		+Mn>+Mg	nHill (for +Mg pH independent)
74	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	nHill				1																																							3 mM MgCl2	8.75		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase		+Mn>+Mg	nHill (for +Mg pH independent)
75	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	0,005-0,5 mM	nHill				1.1																																							3 mM MnCl2	8.75		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase		+Mn>+Mg	nHill (for +Mg pH independent)
76	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	1 mM	nHill				1.9																																							3 mM MgCl2	6.4		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase; var. FBP conc.		+Mg>+Mn	nHill
77	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	1 mM	nHill				1																																							3 mM MnCl2	6.4		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase; var. FBP conc.		+Mg>+Mn	nHill
78	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	1 mM	nHill				1.9																																							3 mM MgCl2	7.5		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase; var. FBP conc.		+Mg>+Mn	nHill
79	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	1 mM	nHill				1																																							3 mM MnCl2	7.5		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase; var. FBP conc.		+Mg>+Mn	nHill
80	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	1 mM	nHill				1																																							3 mM MgCl2	8.75		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase; var. FBP conc.		+Mg=+Mn	nHill
81	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	1 mM	nHill				1																																							3 mM MnCl2	8.75		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase; var. FBP conc.		+Mg=+Mn	nHill
82	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	1 mM	FBP0,5v				0.18																																							3 mM MgCl2	6.4		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase; var. FBP conc.		+Mg>+Mn	FBP0,5v
83	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	1 mM	FBP0,5v				0.0065																																							3 mM MnCl2	6.4		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase; var. FBP conc.		+Mg>+Mn	FBP0,5v
84	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	1 mM	FBP0,5v				0.2																																							3 mM MgCl2	7.5		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase; var. FBP conc.		+Mg>+Mn	FBP0,5v
85	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	1 mM	FBP0,5v				0.02																																							3 mM MnCl2	7.5		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase; var. FBP conc.		+Mg>+Mn	FBP0,5v
86	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	1 mM	FBP0,5v				0.45																																							3 mM MgCl2	8.75		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase; var. FBP conc.		+Mg>+Mn	FBP0,5v
87	14688	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1977_vol481_105-14	1 mM	FBP0,5v				0.23																																							3 mM MnCl2	8.75		80 mM triethanolamine/HCl buffer; 3.3 mM ADP; 13.3 mM KCI; 2.5 µg purified pyruvate kinase; var. FBP conc.		+Mg>+Mn	FBP0,5v
88	820379	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1976_vol438_90-101	1 mM	nHill				1.37																																				1				7.5	25	80 mM triethanolamine/HCl, 3.3 mM ADP, 1 mM Fru-1,6-P2, 13.3 mM KCl, 3.33 mM MgCl2, 0.167 mM NADH, 20 U lactate dehydrogenase and 2.5 µg pyruvate kinase		PEP>ADP	nHill (1 mM FBP)
89	820379	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1976_vol438_90-101	1 mM	nHill				1.7																																				1				7.5	25	80 mM triethanolamine/HCl, 3.3 mM ADP, 0,25 mM Fru-1,6-P2, 13.3 mM KCl, 3.33 mM MgCl2, 0.167 mM NADH, 20 U lactate dehydrogenase and 2.5 µg pyruvate kinase		PEP>ADP	nHill (0,25 mM FBP)
90	820379	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1976_vol438_90-101	var	PEP0,5v				0.18																																								7.5	25	80 mM triethanolamine/HCl, 3.3 mM ADP, 1 mM Fru-1,6-P2, 13.3 mM KCl, 3.33 mM MgCl2, 0.167 mM NADH, 20 U lactate dehydrogenase and 2.5 µg pyruvate kinase		+Phosphate>> no Phosphate	PEP0,5v
91	820379	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1976_vol438_90-101	var	PEP0,5v				5																																							1 mM Phosphate	7.5	25	80 mM triethanolamine/HCl, 3.3 mM ADP, 1 mM Fru-1,6-P2, 13.3 mM KCl, 3.33 mM MgCl2, 0.167 mM NADH, 20 U lactate dehydrogenase and 2.5 µg pyruvate kinase		+Phosphate>> no Phosphate	PEP0,5v
92	820379	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1976_vol438_90-101	1 mM	FBP0,5v				0.16																																								7.5	25	80 mM triethanolamine/HCl, 3.3 mM ADP,var.conc. Fru-1,6-P2, 13.3 mM KCl, 3.33 mM MgCl2, 0.167 mM NADH, 20 U lactate dehydrogenase and 2.5 µg pyruvate kinase		+Phosphate> no Phosphate	FBP0,5v
93	820379	Lactococcus lactis	strain C10	Crow_BiochimBiophysActa_1976_vol438_90-101	1 mM	FBP0,5v				0.93																																							1 mM Phosphate	7.5	25	80 mM triethanolamine/HCl, 3.3 mM ADP,var.conc. Fru-1,6-P2, 13.3 mM KCl, 3.33 mM MgCl2, 0.167 mM NADH, 20 U lactate dehydrogenase and 2.5 µg pyruvate kinase		+Phosphate> no Phosphate	FBP0,5v
94	4214503	Lactococcus lactis	ML3	Collins_JBacteriol_vol120_pp52-8_1974	var	nHill	3.3			2.7																																				1.6				7.5	25	50 mM triethanolamine- hydrochloride, 8 mM MgCl2, 80 mM KCl, 0.12 mM NADH, +- 0.08 mM FDP, 100 µg of lactate dehydrogenase (LDH)		no>FBP>ADP	nHill (+- 0,08 mM FBP)
95	4214503	Lactococcus lactis	ML3	Collins_JBacteriol_vol120_pp52-8_1974	var	nHill				1.7																																				1.2				7.5	25	50 mM triethanolamine- hydrochloride, 8 mM MgCl2, 80 mM KCl, 0.12 mM NADH, 0.2 mM FDP, 100 µg of lactate dehydrogenase (LDH)		FBP>ADP	nHill (+ 0,2 mM FBP)
96	4214503	Lactococcus lactis	ML3	Collins_JBacteriol_vol120_pp52-8_1974	var	nHill				1																																								7.5	25	50 mM triethanolamine- hydrochloride, 8 mM MgCl2, 80 mM KCl, 0.12 mM NADH, 0.8 mM FDP, 100 µg of lactate dehydrogenase (LDH)			nHill (+ 0,8 mM FBP)
97	4214503	Lactococcus lactis	ML3	Collins_JBacteriol_vol120_pp52-8_1974	var	nHill																																								1				7.5	25	50 mM triethanolamine- hydrochloride, 8 mM MgCl2, 80 mM KCl, 0.12 mM NADH, 2 mM FDP, 100 µg of lactate dehydrogenase (LDH)			nHill (+ 2 mM FBP)
98	4214503	Lactococcus lactis	ML3	Collins_JBacteriol_vol120_pp52-8_1974	var	Km				0.17																																				1				7.5	25	50 mM triethanolamine- hydrochloride, 8 mM MgCl2, 80 mM KCl, 0.12 mM NADH, 0.2-5 mM ADP, 2 mM FBP, 100 µg of lactate dehydrogenase (LDH)		ADP>PEP+FBP	Km
99	11395443	Lactococcus lactis	IL1403 lacks the lactose plasmid	Even_J Bacteriol_v183_pp3817-24_2001	6 mM	Km	1																																							4				7.2	30	100 mM Tris-HCl buffer, 5 mM MnSO4, 10 mM KCl, 0.3 mM NADH, 10 U lactate dehydrogenase, 3 mM GDP		ADP>no	Km
100	815244	Streptococcus cremoris	AM2	Thomas_jbacter_vol125_pp1240-42_1976	2 mM	Km	5			0.24						0.14									0.18									0.27																7.5	25	50 mM triethanolamine-hydrochloride, 8 mM MgCl2, 80 mM KCI, 5 mM ADP, 0.12 mM NADH, 2 mM Activator	MANY OTHER ACTIVATORS TESTED	no>R5P>FBP>F6P>G6P	Km
101	815244	Streptococcus cremoris	AM2	Thomas_jbacter_vol125_pp1240-42_1976	2 mM	Vmax [mM NADH/(mg*min)]	2			59						79									92									89																7.5	25	50 mM triethanolamine-hydrochloride, 8 mM MgCl2, 80 mM KCI, 5 mM ADP, 0.12 mM NADH, 2 mM Activator	MANY OTHER ACTIVATORS TESTED	F6P>R5P>G6P>FBP>no	Vmax
102	815244	Streptococcus cremoris	AM2	Thomas_jbacter_vol125_pp1240-42_1976	2 mM	Shalf				0.057						0.012									0.03									0.015															Gal6P	7.5	25	50 mM triethanolamine-hydrochloride, 8 mM MgCl2, 80 mM KCI, 5 mM ADP, 0.12 mM NADH, 2 mM Activator	MANY OTHER ACTIVATORS TESTED	FBP>F6P>R5P>G6P	Shalf
103	1176435	Streptococcus mutans	JC2	Yamada_JBact_vol124_pp562-3_1975	1 mM	Km										0.19																														0.38				7	25	0,05 M tris-HCL, 0.1 M NH4Cl, 0.01 M MgSO4, 0.12 mM reduced nicotinamide adenine dinucleotide, 1 mM adenosine 5'-diphosphate, various concentration of G6P, and 8.3 µg of L-lactate dehydrogenase per ml	enzyme activity was completely inhibited by 20 mM phosphate in the presence of 0.4 mM G6P. The effect of phosphate at concentrations lower than 1.5 mM was negligible	ADP>G6P	Km
104	7054143	Streptococcus mutans	JC2	Abbe_JBact_vol149_pp299-305_1982	1 mM	half-saturation concentrations										0.04																		0.41																7.5		50 mM Tris-hydrochloride buffer, 100 mM KCI, 10mM MgCl2, 0.12 mM NADH, 10 mM 2-mercaptoethanol, 1 mM ADP, 0.5 mM glucose 6-phosphate, 10µg/ml LDH		R5P>G6P	half-saturation concentrations; ADP
105	7054143	Streptococcus mutans	JC2	Abbe_JBact_vol149_pp299-305_1982	1 mM	half-saturation concentrations										0.011																		0.095																7.5		50 mM Tris-hydrochloride buffer, 100 mM KCI, 10mM MgCl2, 0.12 mM NADH, 10 mM 2-mercaptoethanol, 1 mM GDP, 0.5 mM glucose 6-phosphate, 10µg/ml LDH		R5P>G6P	half-saturation concentrations; GDP
106	not found	Lactobacillus delbrueckii subsp. lactis	NCC88	Bourniquel_etal-2002_Purification+Characterization_of_PK_of_Lactobacillus_delbrueckii	0 - 8 mM	Vmax [U/mg]	0,14	0,34		33 fold						54 fold									44 fold																								c [ADP]=1.5 or 2.5 or 5.0 mM	7.4	45	50mM Tris–HCl, 50mM KCl, 7mM MgCl2, 10U/mL of lactate dehydrogenase (LDH), 0.15mM beta-NADH	K+ and Mg2+ were required for PYK activity	G6P>F6P>FBP>none	Vmax	weitere Werte enthalten(z.B nHill ADP bzw. PEP; nHill, Kapp [PEP] bei versch. FBP Konzentrationen
107	6840832	Streptococcus sanguis	NCTC 10904	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	2 mM	nHill	2.4			1																																								7.1		50 mM MOPS- NaOH, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 2 mM dithiothreitol, 3 mM ADP, +/- 0.5 mM FBP, 10 µg LDH		none>FBP	nHill
108	6840832	Streptococcus sanguis	NCTC 10904	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	2 mM	Km	0.9			0.13																																								7.1		50 mM MOPS- NaOH, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 2 mM dithiothreitol, 3 mM ADP, +/- 0.5 mM FBP, 10 µg LDH		none>FBP	Km
109	6840832	Streptococcus sanguis	NCTC 10904	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	2 mM	Vmax [U/mg]	120			167																																								7.1		50 mM MOPS- NaOH, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 2 mM dithiothreitol, 3 mM ADP, +/- 0.5 mM FBP, 10 µg LDH		FBP>none	Vmax
110	6840832	Streptococcus sanguis	NCTC 10904	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	0,5 mM	activity [U/mg]	<0.5			77.2						<0.5									<0.5			<0.5						<0.5									10.9						GMP: 3.9; IMP: 2.6; UMP: 3.3; CMP: 5.1	7.1		50 mM MOPS- NaOH, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 2 mM dithiothreitol, 0.6 mM ADP, 10 µg LDH		FBP>>AMP>CMP>GMP>UMP>IMP>none	activity
111	6840832	Streptococcus mutans	NCTC 10449	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.06			0.05						2.01																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>none>FBP	activity
112	6840832	Streptococcus mutans	JC2	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.13			0.23						1.48																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP>none	activity
113	6840832	Streptococcus mutans	Ingbritt	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.05			0.07						0.66																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP>none	activity
114	6840832	Streptococcus mutans	PK1	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.02			0.02						0.32																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP=none	activity
115	6840832	Streptococcus mutans	GS5	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.03			0.04						0.72																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP>none	activity
116	6840832	Streptococcus mutans	PS14R	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	<0.01			<0.01						0.46																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP and G6P>none	activity
117	6840832	Streptococcus mutans	PS14M	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.06			0.06						0.65																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP=none	activity
118	6840832	Streptococcus mutans	LM7	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.01			0.01						0.08																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP=none	activity
119	6840832	Streptococcus mutans	ATCC 19645	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	<0.01			<0.01						0.61																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP and G6P>none	activity
120	6840832	Streptococcus mutans	BHT-2	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.07			0.05						1.14																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>none>FBP	activity
121	6840832	Streptococcus mutans	JH145	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.26			0.14						1.19																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>none>FBP	activity
122	6840832	Streptococcus mutans	FIL	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.09			0.14						0.84																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP>none	activity
123	6840832	Streptococcus mutans	HS6	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.02			0.08						0.56																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP>none	activity
124	6840832	Streptococcus mutans	6715	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.03			0.07						0.85																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP>none	activity
125	6840832	Streptococcus mutans	C67-1 ruw	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	<0.01			<0.01						0.44																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP and G6P>none	activity
126	6840832	Streptococcus mutans	C67-1 glad	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.01			0.01						0.27																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP=none	activity
127	6840832	Streptococcus salivarius	NCTC 8618	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	<0.01			0.01						4.31																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>>FBP>none	activity
128	6840832	Streptococcus salivarius	13419	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	<0.01			<0.01						4.23																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP and G6P>none	activity
129	6840832	Streptococcus salivarius	ATCC 9759	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.06			0.02						0.3																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>none>FBP	activity
130	6840832	Streptococcus bovis	BM	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.02			0.13						0.53																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP>none	activity
131	6840832	Streptococcus bovis	D95	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.03			0.02						0.36																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>none>FBP	activity
132	6840832	Streptococcus lactis	C10	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.03			0.09						1.1																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		G6P>FBP>none	activity
133	6840832	Streptococcus mitis	NCTC 10712	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.04			0.35						0.04																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		FBP>G6P=none	activity
134	6840832	Streptococcus mitis	115B2	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.12			0.46						0.17																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		FBP>G6P>none	activity
135	6840832	Streptococcus sanguis	NCTC 10904	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.06			0.25						0.05																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		FBP>none>G6P	activity
136	6840832	Streptococcus sanguis	ATCC 10556	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	<0.01			0.95						<0.01																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		FBP>none and FBP>G6P	activity
137	6840832	Streptococcus sanguis	ATCC 10557	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.03			0.56						0.04																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		FBP>G6P>none	activity
138	6840832	Streptococcus sanguis	LPH1	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.02			0.63						0.08																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		FBP>G6P>none	activity
139	6840832	Streptococcus sanguis	GSG2	Abbe_Takahashi_Yamada-1983_Purification+Properties_of_PK_S.sanguis_and_activator_specificita	1 mM	PYK [U/mg]	0.09			0.26						0.08																																		7		67 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.12 mM NADH, 1 mM ADP, 10 µg LDH, +/- 0.4 mM FBP or G6P		FBP>none>G6P	activity
140	11294847	Synechococcus	PCC 6301	Knowles_JBC_vol276_pp20966-72_2001	2,5 mM	Vmax [U/mg]	226			220						248																		246						212									Pi: 224	7	24	50 mM HEPES- KOH, 1 mM ADP, 30 mM MgCl2, 0.15 mM NADH, and 2 units/ml desalted rabbit muscle lactate dehydrogenase	OTHER ACTIVATORS TESTED	G6P>R5P>no>Pi>FBP>ATP	Vmax	Glycerol-3-P: 251; Citrate: 216
141	11294847	Synechococcus	PCC 6301	Knowles_JBC_vol276_pp20966-72_2001	2,5 mM	S0,5 or Km	0.54			0.78						0.074																		0.13						0.75									Pi: 0.90	7	24	50 mM HEPES- KOH, 1 mM ADP, 30 mM MgCl2, 0.15 mM NADH, and 2 units/ml desalted rabbit muscle lactate dehydrogenase	OTHER ACTIVATORS TESTED	Pi>FBP>ATP>no>R5P>G6P	Shalf or Km	Glycerol-3-P: 0,09; Citrate: 0,76
142	370115	Saccharomyces cerevisiae		Yun_JBC_vol254_pp1806-10_1979	10 µM	nHill	2.35			1																																				1				6.2	30	100 µM Mes/ Tris, 10 µM ADP; 100 µM KCl; 24 µM MgCl2; 33 µg/ml LDH; 0.15 µM NADH; + - 1 mM FBP		no>FBP=ADP	nHill
143	370115	Saccharomyces cerevisiae		Yun_JBC_vol254_pp1806-10_1979	10 µM	Km	1.3			0.087		0.003																																						6.2	30	100 µM Mes/ Tris, 10 µM ADP; 100 µM KCl; 24 µM MgCl2; 33 µg/ml LDH; 0.15 µM NADH; + - 1 mM FBP		no<+FBP	Km
144	1096944	Saccharomyces cerevisiae	ATCC 7753	Hirai_BiochBiophysActa_vol391_pp282-91_1975	1 mM	K0,5	4.5			0.15																																								6.2	30	50 mM potassium phosphate buffer, 1 mM ADP , 0.1 mM NADH, 10 mM MgCl2, 0.1 M KCl , + - 1 mM FBP, 2 U/ml LDH		no>>FBP	K0,5
145	1096944	Saccharomyces carlsbergensis	ATCC 9080	Hirai_BiochBiophysActa_vol391_pp282-91_1975	1 mM	K0,5	4.2			0.14																																								6.2	30	50 mM potassium phosphate buffer, 1 mM ADP , 0.1 mM NADH, 10 mM MgCl2, 0.1 M KCl , + - 1 mM FBP, 2 U/ml LDH		no>>FBP	K0,5
146	1096944	Candida tropicalis	Berkhout strain pK 233	Hirai_BiochBiophysActa_vol391_pp282-91_1975	1 mM	K0,5	0.6			0.13																																								7.4	30	50 mM triethanolamine • HCl , 1 mM ADP , 0.1 mM NADH, 10 mM MgCl2, 0.1 M KCl , + - 1 mM FBP, 2 U/ml LDH		no>FBP	K0,5
147	1096944	Candida utilis	ATCC 9226	Hirai_BiochBiophysActa_vol391_pp282-91_1975	1 mM	K0,5	0.62			0.14																																								7.4	30	50 mM triethanolamine • HCl , 1 mM ADP , 0.1 mM NADH, 10 mM MgCl2, 0.1 M KCl , + - 1 mM FBP, 2 U/ml LDH		no>FBP	K0,5
148	1096944	Candida lipolytica	NRRL Y-6795	Hirai_BiochBiophysActa_vol391_pp282-91_1975	1 mM	K0,5	0.16			0.12																																								7.4	30	50 mM triethanolamine • HCl , 1 mM ADP , 0.1 mM NADH, 10 mM MgCl2, 0.1 M KCl , + - 1 mM FBP, 2 U/ml LDH		no>FBP	K0,5
149	4737474	Saccharomyces carlsbergensis		Johannes_JMolBiol_vol76_pp181-205_1973		Vmax [U/mg]	88			96																														86										7	25	0.1 M β-β-di-methylglutaric acid, 0.1 M-KCl, 10 mM ADP, 100 mM MgSO4, 0.25 mM NADH, 36 U/ml LDH, + - 10 mM FBP/ + - 23.4 mM ATP		FBP>no>ATP	Vmax
150	4737474	Saccharomyces carlsbergensis		Johannes_JMolBiol_vol76_pp181-205_1973		K0,5 [x10^3 M^-1]	2.9			0.21																														17										7	25	0.1 M β-β-di-methylglutaric acid, 0.1 M-KCl, 10 mM ADP, 100 mM MgSO4, 0.25 mM NADH, 36 U/ml LDH, + - 10 mM FBP/ + - 23.4 mM ATP		ATP>no>FBP	K0,5
151	4737474	Saccharomyces carlsbergensis		Johannes_JMolBiol_vol76_pp181-205_1973		nHill	2.46			1.07																														1.88										7	25	0,1 M β-β-di-methylglutaric acid, 0.1 M-KCl, 10 mM ADP, 100 mM MgSO4, 0.25 mM NADH, 36 U/ml LDH, + - 10 mM FBP/ + - 23.4 mM ATP		no>ATP>FBP	nHill
152	11112519	Saccharomyces cerevisiae	WT	Bond_Biochem_vol39_pp15333-43_2000	750 µM	Km [µM]	1150		7	50		0.5													410		40	271		21	450		10																	6.2	26	100 mM MES, 200 mM KCl, 15 mM MgCl2, 5% glycerol, 5 mM ADP		no>>RBP>F6P>F1P>FBP	Km
153	11112519	Saccharomyces cerevisiae	WT	Bond_Biochem_vol39_pp15333-43_2000	750 µM	nHill	3.7		0.06	1															3.2		0.3	2.1		0.2	1.6		0.12																	6.2	26	100 mM MES, 200 mM KCl, 15 mM MgCl2, 5% glycerol, 5 mM ADP		no>F6P>F1P>RBP>FBP	nHill
154	11112519	Saccharomyces cerevisiae	mutant T403E	Bond_Biochem_vol39_pp15333-43_2000	750 µM	Km [µM]	960		80	830		60													960		80	834		16																				6.2	26	100 mM MES, 200 mM KCl, 15 mM MgCl2, 5% glycerol, 5 mM ADP		no=F6P>FBP>=F1P	Km
155	11112519	Saccharomyces cerevisiae	mutant T403E	Bond_Biochem_vol39_pp15333-43_2000	750 µM	nHill	2.7		0.2	2.6		0.04													2.6		0.2	2.8		0.1																				6.2	26	100 mM MES, 200 mM KCl, 15 mM MgCl2, 5% glycerol, 5 mM ADP		F1P>=no=F6P>FBP	nHill
156	7646433	Saccharomyces cerevisiae	WT	Collins_Biochem_vol310_pp117-23_1995	0-5,5µM	S0,5	2.76		0.08	0.22		0.004																																						6.5	25	50 µmol of Mes/tetrapropylammonium, 2.4 µmol ADP, 100 µmol KCI, 0.5 pmol pyruvate kinase, 1.1 units/ml lactate dehydrogenase, + - 5 µM FBP		no>FBP	S0,5
157	7646433	Saccharomyces cerevisiae	WT	Collins_Biochem_vol310_pp117-23_1995	0-5,5µM	nHill	2.93		0.07	1.72		0.05																																						6.5	25	50 µmol of Mes/tetrapropylammonium, 2.4 µmol ADP, 100 µmol KCI, 0.5 pmol pyruvate kinase, 1.1 units/ml lactate dehydrogenase, + - 5 µM FBP		no>FBP	S0,5
158	7646433	Saccharomyces cerevisiae	WT	Collins_Biochem_vol310_pp117-23_1995	0-5,5µM	kcat [1/s]	188		5	195		2																																						6.5	25	50 µmol of Mes/tetrapropylammonium, 2.4 µmol ADP, 100 µmol KCI, 0.5 pmol pyruvate kinase, 1.1 units/ml lactate dehydrogenase, + - 5 µM FBP		FBP>no	kcat
159	7646433	Saccharomyces cerevisiae	WT	Collins_Biochem_vol310_pp117-23_1995	5,6 µM	S0,5 [ADP]-FBP																																								0.25		0.006		6.5	25	50 µmol of Mes/tetrapropylammonium, 0-1.6 µmol ADP, 100 µmol KCI, 0.5 pmol pyruvate kinase, 1.1 units/ml lactate dehydrogenase		- FBP > +FBP	S0,5 [ADP]
160	7646433	Saccharomyces cerevisiae	WT	Collins_Biochem_vol310_pp117-23_1995	5,6 µM	S0,5 [ADP]+FBP																																								0.15		0.1		6.5	25	50 µmol of Mes/tetrapropylammonium, 0-1.6 µmol ADP, 100 µmol KCI, 0.5 pmol pyruvate kinase, 1.1 units/ml lactate dehydrogenase		- FBP > +FBP
161	7646433	Saccharomyces cerevisiae	WT	Collins_Biochem_vol310_pp117-23_1995	5,6 µM	nHill [ADP]-FBP																																								1.53		0.02		6.5	25	50 µmol of Mes/tetrapropylammonium, 0-1.6 µmol ADP, 100 µmol KCI, 0.5 pmol pyruvate kinase, 1.1 units/ml lactate dehydrogenase		- FBP > +FBP	nHill[ADP]
162	7646433	Saccharomyces cerevisiae	WT	Collins_Biochem_vol310_pp117-23_1995	5,6 µM	nHill [ADP]+FBP																																								1.01		0.07		6.5	25	50 µmol of Mes/tetrapropylammonium, 0-1.6 µmol ADP, 100 µmol KCI, 0.5 pmol pyruvate kinase, 1.1 units/ml lactate dehydrogenase		- FBP > +FBP
163	9184162	Saccharomyces cerevisiae		Mesecar_Biochem_vol36_pp6792-6802_1997	5 mM	Km [µM]	56		2	21		3																																						6.2	24 + - 1	100 mM MES, + - 1 mM FBP, 5 % glycerol, 200 mM KCl, 15 mM MgCl2, 5 mM ADP, 175 µM NADH, 20 µg 0.5-0.7 µg LDH, 0.5-0.7 µg YPK		no>FBP	Km
164	9184162	Saccharomyces cerevisiae		Mesecar_Biochem_vol36_pp6792-6802_1997	5 mM	nHill	2.3		0.1	1																																								6.2	24 + - 1	100 mM MES, + - 1 mM FBP, 5 % glycerol, 200 mM KCl, 15 mM MgCl2, 5 mM ADP, 175 µM NADH, 20 µg 0.5-0.7 µg LDH, 0.5-0.7 µg YPK		no>FBP	nHill
165	11747307	Saccharomyces cerevisiae	WT	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		Vmax [U/mg]	301		4	353		4																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		FBP>no	Vmax
166	11747307	Saccharomyces cerevisiae	WT	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		S0,5	2.5		0.5	0.23		0.01																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	S0,5
167	11747307	Saccharomyces cerevisiae	WT	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		nHill	2.7		0.1	1.1		0.1																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	nHill
168	11747307	Saccharomyces cerevisiae	mutant R77Q	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		Vmax [U/mg]	256		5	292		3																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		FBP>no	Vmax
169	11747307	Saccharomyces cerevisiae	mutant R77Q	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		S0,5	3.5		0.1	0.28		0.01																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	S0,5
170	11747307	Saccharomyces cerevisiae	mutant R77Q	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		nHill	2.4		0.1	0.86		0.04																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	nHill
171	11747307	Saccharomyces cerevisiae	mutant K236Q	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		Vmax [U/mg]	393		12	399		9																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		FBP=no	Vmax
172	11747307	Saccharomyces cerevisiae	mutant K236Q	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		S0,5	1.6		0.1	0.33		0.03																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	S0,5
173	11747307	Saccharomyces cerevisiae	mutant K236Q	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		nHill	2.1		0.2	0.75		0.04																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	nHill
174	11747307	Saccharomyces cerevisiae	mutant K292Q	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		Vmax [U/mg]	140		5	172		3																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		FBP>no	Vmax
175	11747307	Saccharomyces cerevisiae	mutant K292Q	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		S0,5	3.6		0.2	0.25		0.08																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	S0,5
176	11747307	Saccharomyces cerevisiae	mutant K292Q	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		nHill	2.6		0.2	0.97		0.07																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	nHill
177	11747307	Saccharomyces cerevisiae	mutant E392A	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		Vmax [U/mg]	300		2	278		3																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	Vmax
178	11747307	Saccharomyces cerevisiae	mutant E392A	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		S0,5	0.3		0.01	0.21		0.01																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	S0,5
179	11747307	Saccharomyces cerevisiae	mutant E392A	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		nHill	1.3		0.1	0.94		0.04																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	nHill
180	11747307	Saccharomyces cerevisiae	mutant T403E	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		Vmax [U/mg]	328		13	312		10																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	Vmax
181	11747307	Saccharomyces cerevisiae	mutant T403E	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		S0,5	1.9		0.2	1.5		0.1																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	S0,5
182	11747307	Saccharomyces cerevisiae	mutant T403E	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		nHill	1.6		0.2	2.1		0.3																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		FBP>no	nHill
183	11747307	Saccharomyces cerevisiae	mutant T403K	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		Vmax [U/mg]	305		5	311		5																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		FBP>=no	Vmax
184	11747307	Saccharomyces cerevisiae	mutant T403K	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		S0,5	2.7		0.1	0.28		0.02																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	S0,5
185	11747307	Saccharomyces cerevisiae	mutant T403K	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		nHill	2.5		0.1	1		0.1																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	nHill
186	11747307	Saccharomyces cerevisiae	mutant T406R	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		Vmax [U/mg]	334		6	333		8																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		FBP=no	Vmax
187	11747307	Saccharomyces cerevisiae	mutant T406R	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		S0,5	2.2		0.1	0.27		0.03																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	S0,5
188	11747307	Saccharomyces cerevisiae	mutant T406R	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		nHill	2		0.1	0.87		0.08																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	nHill
189	11747307	Saccharomyces cerevisiae	mutant R409Q	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		Vmax [U/mg]	168		4	185		2																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		FBP>no	Vmax
190	11747307	Saccharomyces cerevisiae	mutant R409Q	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		S0,5	4.1		0.1	0.29		0.02																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	S0,5
191	11747307	Saccharomyces cerevisiae	mutant R409Q	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		nHill	2.4		0.2	1.2		0.1																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	nHill
192	11747307	Saccharomyces cerevisiae	mutant Y436F	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		Vmax [U/mg]	117		3	122		4																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		FBP>no	Vmax
193	11747307	Saccharomyces cerevisiae	mutant Y436F	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		S0,5	3		0.3	0.21		0.03																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	S0,5
194	11747307	Saccharomyces cerevisiae	mutant Y436F	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		nHill	2.3		0.2	0.83		0.1																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	nHill
195	11747307	Saccharomyces cerevisiae	mutant Y436S	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		Vmax [U/mg]	219		5	223		3																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		FBP>=no	Vmax
196	11747307	Saccharomyces cerevisiae	mutant Y436S	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		S0,5	1.3		0.1	0.22		0.01																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	S0,5
197	11747307	Saccharomyces cerevisiae	mutant Y436S	11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002		nHill	2.1		0.2	1		0.1																																						6.2	25	110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP		no>FBP	nHill