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| Item | Value | Unit | |||||||
| Experimentator | Araz Zeyniyev | ||||||||
| Raw data | |||||||||
| Reference | SYSMO-LAB | ||||||||
| Description | Construction of amino acid auxotrophy list for Streptococcus pyogenes in CDM-IMIKRO (not CDM-LAB) as function of final growth yield (OD600 at stationary phase) | ||||||||
| Aim | Construct/refine the amino acid auxotrophy list | ||||||||
| Organism | Streptococcus pyogenes | ||||||||
| Vessel type | 96 wellplate | ||||||||
| Stirring rate | 0 | RPM | |||||||
| Temperature | 37 | degree celcius | |||||||
| Volume | 200 | µl | |||||||
| Gas flow | 0 | ml | |||||||
| pH | |||||||||
| SOP's used | |||||||||
| Explanations | Format | Data | |||||||
| TOPIC | |||||||||
| Title | text | ||||||||
| Comments | text | ||||||||
| ASSAY | |||||||||
| Title | text | Growth measurement | |||||||
| Assay_type | integer | Turbidity screening | |||||||
| Comments | |||||||||
| EXPERIMENT | |||||||||
| Experiment_description | text | OD at 600 nm | |||||||
| Experiment_type | text | ||||||||
| Experimentalist | text | Araz Zeyniyev | |||||||
| Begin_date | date | 2011-08-15T0:0:0+0000 | |||||||
| User | integer | Ara Zeyniyev | |||||||
| Group | integer | IMIKRO | |||||||
| SOP | 1 | ||||||||
| Organism of Origin | |||||||||
| Organism | integer | S.pyogenes | |||||||
| NCBI_ID | integer | 294934 | |||||||
| Strain | text | M49 | |||||||
| Genotype | text | wild-type | |||||||
| Phenotype | text | wild-type | |||||||
| Experimental_conditions | |||||||||
| Item | text | temperature | |||||||
| Start_value | float/text | 37 | |||||||
| End_value | float/text | 37 | |||||||
| Unit | text | celsius degrees | |||||||
| Comments | |||||||||
| FACTORS_STUDIED | |||||||||
| Item | text | OD | |||||||
| Start_value | float/text | 0 | |||||||
| End_value | float/text | 1.2 | |||||||
| Unit | text | 0 | |||||||
| Comments | |||||||||
| DATA_CREATED | |||||||||
| Datatype | text | OD | |||||||
| Creation_date | date | ||||||||
| Status | text | normalised | |||||||
| Person_created | text | Araz Zeyniyev | |||||||
| DATA_FILE | |||||||||
| File_name | text | ||||||||
| File_type | text | xlsx | |||||||
| Storage_location | text | SYSMOSEEK | |||||||
| Storage_date | date | 2012-04-05T0:0:0+0000 | |||||||
| Last_change_date | date | 2012-04-05T0:0:0+0000 | |||||||
| TemplateID | text | ||||||||
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| Identifer | Number of biological replicates | CDM-IMIKRO without | average OD600 | standard deviation | |||||
| unit | 1.0 | amino acid abbreviated name | 1 | 1 | |||||
| 1 | asn | 0.2532272727272727 | 0.004671792948178392 | ||||||
| 1 | asn-asp | 0.2552727272727273 | 0.04473759399003584 | ||||||
| 1 | cyn-cys | 0.4591181818181819 | 0.019757696182540075 | ||||||
| 1 | cys-gly | 0.04040454545454546 | 9.862035300065618E-4 | ||||||
| 1 | cys-ser | 0.05680909090909091 | 0.005730965105369344 | ||||||
| 1 | cys-thr | 0.02840454545454546 | 8.054736983836769E-4 | ||||||
| 1 | gln-glu | 0.08470454545454544 | 0.0038466097964840323 | ||||||
| 1 | gln-thr | 0.04356818181818182 | 0.005595417884188928 | ||||||
| 1 | gly-ser | 0.06542272727272729 | 0.00468728592584764 | ||||||
| 1 | gly-ser-thr | 0.07721363636363635 | 0.003947940446135649 | ||||||
| 1 | gly-thr | 0.03511363636363637 | 9.77628546922386E-4 | ||||||
| 1 | hpro | 0.5717472727272727 | 0.09053728875996055 | ||||||
| 1 | met-ser | 0.07773636363636363 | 0.006838357882426056 | ||||||
| 1 | pro-hpro | 0.3399045454545455 | 0.02047944910142481 | ||||||
| 1 | ser-thr | 0.09036363636363634 | 0.00880031360125987 | ||||||
| 2 | ala | 0.5169854545454546 | 0.14550398280771148 | ||||||
| 2 | asp | 0.6186909090909092 | 0.04056853303503783 | ||||||
| 2 | cyn | 0.5965036363636361 | 0.06687977110328905 | ||||||
| 2 | gln | 0.052758585858585894 | 0.03658728384010107 | ||||||
| 2 | glu | 0.5070766233766234 | 0.09401287014744594 | ||||||
| 2 | gly | 0.06344181818181818 | 0.026469224670505345 | ||||||
| 2 | ile | 0.040447272727272734 | 0.008463245252385187 | ||||||
| 2 | phe | 0.06504545454545457 | 0.031243777068392934 | ||||||
| 2 | pro | 0.14803636363636363 | 0.1471681059201084 | ||||||
| 2 | ser | 0.05411818181818182 | 0.021565697850834585 | ||||||
| 2 | thr | 0.040859999999999994 | 0.005369817915390097 | ||||||
| 3 | arg | 0.03823553719008263 | 0.0224587793134598 | ||||||
| 3 | cys | 0.42480000000000007 | 0.16707694655291158 | ||||||
| 3 | full | 0.5980433155080214 | 0.11612676342502623 | ||||||
| 3 | his | 0.05718760330578511 | 0.017931267633109885 | ||||||
| 3 | leu | 0.03746446280991736 | 0.01941642178459654 | ||||||
| 3 | lys | 0.0338206611570248 | 0.024227740645414126 | ||||||
| 3 | met | 0.056079338842975175 | 0.015007073336090082 | ||||||
| 3 | trp | 0.055525619834710736 | 0.018268126974440236 | ||||||
| 3 | tyr | 0.03490909090909092 | 0.030038324363312205 | ||||||
| 3 | val | 0.04091652892561984 | 0.022411536329065358 |
