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Organism_Sample
Data_Calc_qATP_at_Mumax
Data_qATP_average_and_SD
Results_calculations
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| Asset Title | Calculation of qATP for assessment of maintenance and qATP at the maximal growth rate | ||||||||
| Uploader | Martijn Bekker | ||||||||
| Uploader SEEK ID | 53 | ||||||||
| Project | SysMO-LAB | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 107, 109 | ||||||||
| Assay Title | Assessment of qATP for three different lactic acid bacteria at two different pH's at various dilution rates | ||||||||
| Assay_type | organism_or_strain_characterisation | ||||||||
| Technology_type | Batch | ||||||||
| Description | Assessment of qATP for three different lactic acid bacteria at two different pH's at various dilution rates | ||||||||
| Experimentalist | Martijn Bekker | ||||||||
| Date | 01/12/2007 - 01/01/2010 | ||||||||
| SOP | 2 | ||||||||
| Publication | PMID:21097579 | ||||||||
| Experimental_conditions | |||||||||
| Item | |||||||||
| Value | |||||||||
| Unit | |||||||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| Comments | |||||||||
| Culture growth | Chemostat | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | pH | Dilution_rate | |||||||
| Value | |||||||||
| Unit | 1/h | ||||||||
| Start_value (optional) | 6.5 | 0.05 | |||||||
| End_value (optional) | 7.5 | 0.4 | |||||||
| SD (optional) | |||||||||
| Comments | |||||||||
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| SAMPLE Name | Treatments (Factors Studied) | ||||||||
| Organism | NCBI_ID | Strain | Genotype | Phenotype | organism/cell part | pH | DillutionRate | ||
| NZ9000_a | Lactococus Lactis | 1358 | NZ9000 | Wild-type | Whole Cell | 6.5 | 0.05-0.4 | ||
| NZ9010_a | Lactococus Lactis | 1358 | NZ9010 | Δldh | Whole Cell | 6.5 | |||
| v538_a | Enterococcus_faecalis | 1351 | V538 | Wild-type | Whole Cell | 6.5 | 0.05-0.4 | ||
| V538Δldh_a | Enterococcus_faecalis | 1351 | V538 | Δldh | Whole Cell | 6.5 | |||
| M49_a | Streptococcus_pyogenes | 1314 | M49 | Wild-type | Whole Cell | 6.5 | 0.05-0.15 | ||
| M49Δldh_a | Streptococcus_pyogenes | 1314 | M49 | Δldh | Whole Cell | 6.5 | |||
| NZ9000_b | Lactococcus_lactis | 1358 | NZ9000 | Wild-type | Whole Cell | 7.5 | 0.05-0.15 | ||
| NZ9010_b | Lactococus Lactis | 1358 | NZ9010 | Δldh | Whole Cell | 7.5 | |||
| v538_b | Enterococcus_faecalis | 1351 | V538 | Wild-type | Whole Cell | 7.5 | 0.05-0.4 | ||
| V538Δldh_b | Enterococcus_faecalis | 1351 | V538 | Δldh | Whole Cell | 7.5 | |||
| M49_b | Streptococcus_pyogenes | 1314 | M49 | Wild-type | Whole Cell | 7.5 | 0.05-0.15 | ||
| M49Δldh_b | Streptococcus_pyogenes | 1314 | M49 | Δldh | Whole Cell | 7.5 | |||
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| Sample | NZ9000_a | NZ9010_a | V538_a | V538Δldh_a | M49_a | M49Δldh_a | NZ9000_b | NZ9010_b | V538_b | V538Δldh_b | M49_b | M49Δldh_b |
| qATP calculated at mumax | ||||||||||||
| h-1 | 0.49 | 0.49 | 0.79 | 0.89 | 0.39 | 0.35 | 0.43 | 0.45 | 0.74 | 0.63 | 0.43 | 0.39 |
| mmol/gram dryweight cells/h | 8.2 | 8.2 | 2 | 2 | 2.9 | 2.9 | 5 | 5 | 2 | 2 | -2.6 | -2.6 |
| mmol/gram dryweight cells/h | 3.4158441845071956 | 3.4158441845071956 | 0.7693545684277037 | 0.7693545684277037 | 2.1483202538373964 | 2.1483202538373964 | 1.4723348461727495 | 1.4723348461727495 | 1.2355581159434603 | 1.2355581159434603 | 2.2433131324883617 | 2.2433131324883617 |
| mmol/gram dryweight cells/h at µ=1.0 | 176 | 176 | 57 | 57 | 91 | 91 | 65 | 65 | 64 | 64 | 211 | 211 |
| mmol/gram dryweight cells/h at µ=1.0 | 30.55223918842169 | 30.55223918842169 | 3.401206296719618 | 3.401206296719618 | 20.417247418451606 | 20.417247418451606 | 6.821124847055777 | 6.821124847055777 | 4.4119781235022835 | 4.4119781235022835 | 21.320042568729278 | 21.320042568729278 |
| mmol/gram dryweight cells/h at µmax | 94.44 | 94.44 | 47.03 | 52.730000000000004 | 38.39 | 34.75 | 32.95 | 34.25 | 49.36 | 42.32 | 88.13000000000001 | 79.69000000000001 |
| mmol/gram dryweight cells/h at µmax | 14.970597202326628 | 14.970597202326628 | 2.686952974408498 | 3.02707360408046 | 7.962726493196127 | 7.146036596458061 | 2.933083684233984 | 3.0695061811750994 | 3.26486381139169 | 2.7795462178064385 | 9.167618304553589 | 8.314816601804418 |
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| Sample | Dilution rate | qATP | Yatp | ||||||
| Unit | /hour | mmol/gram/hour | [dw]/mol ATP | ||||||
| M49_a | 0.05 | 5.708333333333334 | 8.759124087591239 | ||||||
| M49_a | 0.15 | 25.221428571428575 | 5.94732370433305 | ||||||
| M49_a | 0.05 | 9.155136263736265 | 5.46141516189675 | ||||||
| M49_a | 0.15 | 29.2 | 5.136986301369864 | ||||||
| M49_a | 0.05 | 8.089040816326325 | 6.18120258449972 | ||||||
| M49_a | 0.05 | 9.086708029197638 | 5.502542817414045 | ||||||
| M49_a | 0.15 | 33.11899619771752 | 4.529122776080322 | ||||||
| M49_b | 0.05 | 8.746153846153847 | 5.716798592788039 | ||||||
| M49_b | 0.15 | 12.54 | 11.961722488038278 | ||||||
| M49_b | 0.05 | 4.893127777777779 | 10.218412898816137 | ||||||
| M49_b | 0.05 | 8.08156768558952 | 6.186918422913967 | ||||||
| M49_b | 0.15 | 17.635596868884537 | 8.505524429663808 | ||||||
| M49_b | 0.15 | 19.6 | 7.653061224489795 | ||||||
| M49_b | 0.05 | 8.2 | 6.097560975609757 | ||||||
| NZ9000_a | 0.05 | 7.989175257731959 | 6.258468288276664 | ||||||
| NZ9000_a | 0.05 | 7.869148936170213 | 6.353927267811275 | ||||||
| NZ9000_a | 0.15 | 20.671428571428574 | 7.256392536281962 | ||||||
| NZ9000_a | 0.15 | 16.677669902912623 | 8.994062172546279 | ||||||
| NZ9000_a | 0.15 | 16.74375 | 8.958566629339305 | ||||||
| NZ9000_a | 0.05 | 4.217160220994474 | 11.85631974594724 | ||||||
| NZ9000_a | 0.05 | 6.807896 | 7.3444130168851 | ||||||
| NZ9000_a | 0.25 | 19.59890134529148 | 12.755817052982973 | ||||||
| NZ9000_a | 0.3 | 25.21497247706422 | 11.897692939101278 | ||||||
| NZ9000_a | 0.35 | 25.471747081712067 | 13.740714324667946 | ||||||
| NZ9000_a | 0.4 | 30.654541436464083 | 13.048637534802378 | ||||||
| NZ9000_b | 0.15 | 38.96730769230769 | 3.849380644524503 | ||||||
| NZ9000_b | 0.05 | 13.6675 | 3.658313517468448 | ||||||
| NZ9000_b | 0.15 | 31.371875 | 4.781352724374938 | ||||||
| NZ9000_b | 0.15 | 33.519375 | 4.475023773563798 | ||||||
| NZ9000_b | 0.05 | 20.757657534246967 | 2.408749634562938 | ||||||
| NZ9000_b | 0.05 | 16.650772727272727 | 3.0028636399621096 | ||||||
| v538_a | 0.05 | 6.0991928571428575 | 8.19780603288257 | ||||||
| v538_a | 0.05 | 5.882994642857144 | 8.499072842214408 | ||||||
| v538_a | 0.05 | 5.881375 | 8.501413359971096 | ||||||
| v538_a | 0.05 | 5.612043560606061 | 8.909410531125733 | ||||||
| v538_a | 0.05 | 4.595565068493151 | 10.880054847399776 | ||||||
| v538_a | 0.05 | 2.9057835820895526 | 17.207062600321027 | ||||||
| v538_a | 0.05 | 2.822826086956522 | 17.71274547554871 | ||||||
| v538_a | 0.15 | 14.375347602739724 | 10.434530290691006 | ||||||
| v538_a | 0.15 | 14.197237261146494 | 10.56543588311398 | ||||||
| v538_a | 0.15 | 13.214879166666664 | 11.35084158607832 | ||||||
| v538_a | 0.15 | 12.85094691780822 | 11.672291618615064 | ||||||
| v538_a | 0.15 | 12.877931506849315 | 11.64783334343876 | ||||||
| v538_a | 0.15 | 9.681099683544304 | 15.494107581080517 | ||||||
| v538_a | 0.15 | 8.45197543352601 | 17.74733033475261 | ||||||
| v538_a | 0.15 | 11.413201754385966 | 13.142674880197983 | ||||||
| v538_a | 0.4 | 31.100095238095243 | 12.86169694779682 | ||||||
| v538_a | 0.4 | 30.44022891566265 | 13.14050564824054 | ||||||
| v538_a | 0.4 | 26.511699029126213 | 15.08767882286809 | ||||||
| v538_a | 0.4 | 29.08322388059702 | 13.753633422560883 | ||||||
| v538_a | 0.4 | 24.736390909090908 | 16.17050771351594 | ||||||
| v538_a | 0.4 | 29.534444444444446 | 13.543508521124112 | ||||||
| v538_a | 0.4 | 33.74509090909091 | 11.85357600836216 | ||||||
| v538_a | 0.4 | 24.736390909090908 | 16.17050771351594 | ||||||
| v538_a | 0.4 | 29.53444444444444 | 13.543508521124114 | ||||||
| v538_a | 0.4 | 33.74509090909092 | 11.853576008362156 | ||||||
| v538_a | 0.4 | 20.075 | 19.925280199252807 | ||||||
| v538_a | 0.4 | 18.811695906432746 | 21.2633673215618 | ||||||
| v538_b | 0.05 | 5.280526073619632 | 9.468753548967253 | ||||||
| v538_b | 0.05 | 3.8407239884393065 | 13.018378865677796 | ||||||
| v538_b | 0.05 | 4.013751262626263 | 12.457174530362948 | ||||||
| v538_b | 0.05 | 4.143242610837439 | 12.067842676944743 | ||||||
| v538_b | 0.05 | 5.046419708029195 | 9.90801457129034 | ||||||
| v538_b | 0.05 | 5.150467391304348 | 9.7078568217743 | ||||||
| v538_b | 0.15 | 11.921674876847291 | 12.582124705590678 | ||||||
| v538_b | 0.15 | 12.638423645320199 | 11.868568755846583 | ||||||
| v538_b | 0.15 | 13.441285714285712 | 11.15964671746963 | ||||||
| v538_b | 0.15 | 8.11055957943925 | 18.49440825023453 | ||||||
| v538_b | 0.15 | 8.853698394495412 | 16.942072489532634 | ||||||
| v538_b | 0.4 | 27.1641 | 14.725317606694128 | ||||||
| v538_b | 0.4 | 25.361896 | 15.771691517069545 | ||||||
| v538_b | 0.4 | 24.253561904761906 | 16.4924229097032 | ||||||
| v538_b | 0.4 | 21.204439024390247 | 18.863974639456533 |
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| Organism | L.lactis | Note: I haven't changed the results file | |||||||
| Calculations | Units | Units | |||||||
| pH 6.5 | Average slope | 65.04342930337994 | 5.0074276443798915 | Crossing with Y-axis | |||||
| stdev of slope | 6.821124847055777 | 1.4723348461727495 | Stdev of crossing | ||||||
| pH 7.5 | Average slope | 175.94209143596 | 8.228205515375226 | Crossing with Y-axis | |||||
| stdev of slope | 30.55223918842169 | 3.4158441845071956 | Stdev of crossing | ||||||
| Organism | E. faecalis | ||||||||
| Calculations | Units | Units | |||||||
| pH 6.5 | Average slope | 64.41522026774066 | 1.9955387748083364 | Crossing with Y-axis | |||||
| stdev of slope | 4.4119781235022835 | 1.2355581159434603 | Stdev of crossing | ||||||
| pH 7.5 | Average slope | 56.58293627222223 | 2.0319992702004868 | Crossing with Y-axis | |||||
| stdev of slope | 3.401206296719618 | 0.7693545684277037 | Stdev of crossing | ||||||
| Organism | S.pyogenes | ||||||||
| Calculations | Units | Units | |||||||
| pH 6.5 | Average slope | 211.70336979066965 | -2.575363878885092 | Crossing with Y-axis | |||||
| stdev of slope | 21.320042568729278 | 2.2433131324883617 | Stdev of crossing | ||||||
| pH 7.5 | Average slope | 91.11653295581226 | 2.9243856795896725 | Crossing with Y-axis | |||||
| stdev of slope | 20.417247418451606 | 2.1483202538373964 | Stdev of crossing | ||||||
