Selected Cell
Cell:
Value:
ReadMe
LHY original data
LHY mean and SE for BioDare
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| This is one of many sheets from Andrew Millar's reworking (Nov-Dec 2010) of Aurora PinasFenandez's data (2009-2010). The readme is for all sheets. | |||||||||
| Harvesting - ZT0 and ZT12 were harvested in a darkroom. ZT0 plants were taken before lights came on in the cabinets; ZT12 immediately after lights went out. Harvesting took 3-4 mins for all genotypes, always in same order. | |||||||||
| Calibration standard curves were different for Col and Ws samples - so levels CANNOT be compared across accessions, but relative levels can (Andrew thinks?) | |||||||||
| Aurora had normalised all to a single timepoint in WT | |||||||||
| This copy removes that normalisation… greyed out columns | |||||||||
| Also makes multiple versions of final data for matching to the Pokhilko 2010 model in SBSI | |||||||||
| Aurora's quality control also removes samples with differing GOI measures BEFORE ratioing to ACT (shaded in red) | |||||||||
| would be better to do so AFTER ratioing… still to update, exceppt some manual edits to 7-9- data | |||||||||
| AJM manually edited the 14h to 18h timepoints in the prr7-9- mutant, as many values were omitted when the ratio's looked reasonably close among reps | |||||||||
| For toc1-9, ignore LHY data, much lower than CCA1 in first 4 timepoints; LUX ditto | |||||||||
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| LHY | This Normalisation not used | SE | ||||||||||||||||||
| WS | ZT | GOI Conc | ACT Conc | GOI/UBQ | ratio/chosen sample | Average | SD | SE | Time | WS-2 | toc1-9 | gi-11 | WS-2 | toc1-9 | gi-11 | |||||
| rep1 | 0 | 206.086734623687 | 61.1757801954833 | 3.3687634872027785 | 443.4946705292058 | 4.631160493784372 | 1.8190261579824738 | 1.050215241974152 | 0 | 4.631160493784372 | 0.6514320276726221 | 1.609260049424785 | 1.050215241974152 | 0.18289258912058556 | 0.14588895025669146 | |||||
| rep2 | 0 | 386.294097632889 | 101.428686101068 | 3.8085290511203964 | 501.3894098365588 | 2 | 1.8950559588403362 | 0.0672762043457649 | 1.5237783694931526 | 0.5496823878886727 | 0.008221288037759112 | 0.1473272091071293 | ||||||||
| rep3 | 0 | 360.53271178144 | 53.6811448932807 | 6.71618894302994 | 884.1802084996492 | 4 | 0.3010540455877197 | 0.07340015055508498 | 0.4566242790513498 | 0.0289287247343889 | 0.009279079236991417 | 0.07739795165598891 | ||||||||
| rep1 | 2 | 112.370536724689 | 100.611018254029 | 1.1168810203368464 | 147.0363775955985 | 1.8950559588403362 | 0.9520778238489644 | 0.5496823878886727 | 6 | 0.04606019336454031 | 0 | 0.07326787628515338 | 0.007478250397870409 | 0 | 0.0017650600135167213 | |||||
| rep2 | 2 | 86.4593906421338 | 53.6474359738162 | 1.611622048150301 | 212.16858707273155 | 8 | 0.015348707551106408 | 0.051657401779550416 | 0.022948538635409046 | 0.0028597088907186988 | 0.013112539894701267 | 0.0014933552710307452 | ||||||||
| rep3 | 2 | 152.68324986649 | 51.640364998974 | 2.9566648080338616 | 389.24225161116067 | 10 | 0.006243423509607333 | 0.026482956548317456 | 0.013498187188249022 | 3.2549730765303044E-4 | 7.978520022712282E-4 | 3.5585216870341434E-5 | ||||||||
| rep1 | 4 | 31.5812023458523 | 90.3700234860103 | 0.349465465733128 | 46.00681293756846 | 0.3010540455877197 | 0.050106021038136044 | 0.0289287247343889 | 12 | 0.004997095291601669 | 0.014501845124493427 | 0.009851071596153331 | 0.0014454244284333986 | 0.00405939947828125 | 0.0023227249575557634 | |||||
| rep2 | 4 | 33.0256726132349 | 108.534720785614 | 0.30428670543567077 | 40.05906994844105 | 14 | 0.0024388292135717263 | 0.003814454775239107 | 0.02901617103357374 | 9.287697145538574E-5 | 0.0010453873449936064 | 0.008990378276165266 | ||||||||
| rep3 | 4 | 20.6188194801892 | 82.6703914218231 | 0.24940996559436032 | 32.83459671127492 | 16 | 0 | 0.06458061290082012 | 0.01577924655222094 | 0 | 0.0040161051661944175 | 0.001252218931522611 | ||||||||
| rep1 | 6 | 4.91195819251289 | 85.1120550205428 | 0.057711662482210435 | 7.597688242427806 | 0.04606019336454031 | 0.01295270964083372 | 0.007478250397870409 | 18 | 0.04238813010100739 | 0.5739433106477756 | 0.056676081034892134 | 0.003804008381865387 | 0.1785686234162341 | 0.004749017064173374 | |||||
| rep2 | 6 | 2.61170080839921 | 81.3279983147711 | 0.03211318196091473 | 4.227671401540286 | 20 | 0.181389891763515 | 1.6013707841397113 | 0.29162764244467254 | 0.010749001681596482 | 0.04426731068760343 | 0.0646052149993204 | ||||||||
| rep3 | 6 | 3.59212354005276 | 74.2853663940884 | 0.04835573565049576 | 6.3659889250109 | 22 | 1.056063792336563 | 0.4827831065299017 | 0.09165342239818909 | 0.047238299373526804 | ||||||||||
| rep1 | 8 | 0.976760637437872 | 48.4485517511875 | 0.020160780913620002 | 2.65414859869597 | 0.015348707551106408 | 0.00495316109358122 | 0.0028597088907186988 | 24 | |||||||||||
| rep2 | 8 | 0.688805273850321 | 67.098465872633 | 0.01026558901000536 | 1.3514555216106559 | |||||||||||||||
| rep3 | 8 | 0.946718640746676 | 60.6103474958935 | 0.015619752729693862 | 2.0563263395956723 | |||||||||||||||
| rep1 | 10 | 0.447853288594363 | 79.6384519747178 | 0.005623581040180685 | 0.7403393648985772 | 0.006243423509607333 | 5.637778745819266E-4 | 3.2549730765303044E-4 | ||||||||||||
| rep2 | 10 | 0.5925063194466 | 88.0964047127263 | 0.006725658344159501 | 0.8854268466058912 | |||||||||||||||
| rep3 | 10 | 0.444916906409371 | 69.7249231880221 | 0.00638103114448181 | 0.8400569870247515 | |||||||||||||||
| rep1 | 12 | 0.279491325189807 | 107.446660763272 | 0.0026012099697131233 | 0.34244694318184593 | 0.004997095291601669 | 0.0025035485485478508 | 0.0014454244284333986 | ||||||||||||
| rep2 | 12 | 0.149800925624593 | 45.1578021370588 | 0.0033172767170982108 | 0.43671648374616795 | |||||||||||||||
| rep3 | 12 | 0.34447671766501 | 45.3500487600808 | 0.007595950326038772 | 1 | |||||||||||||||
| rep4 | 12 | 0.26505444309132 | 55.2873383729074 | 0.004794125579053111 | 0.6311423025791704 | |||||||||||||||
| rep1 | 14 | 0.35481976463598 | 43.6415054081423 | 0.008130328257873992 | 1.0703503720927956 | 0.0024388292135717263 | 1.3134787266434533E-4 | 9.287697145538574E-5 | ||||||||||||
| rep2 | 14 | 0.151122651310304 | 59.6920180564656 | 0.002531706185027112 | 0.33329683270156096 | |||||||||||||||
| rep3 | 14 | 0.178943897152468 | 76.2777237916141 | 0.0023459522421163405 | 0.30884249388446644 | |||||||||||||||
| rep1 | 16 | 0.172393463146328 | 64.931068528619 | 0.0026550227349230195 | 0.3495313451197347 | 0 | ||||||||||||||
| rep2 | 16 | 5.38014192488734 | 44.5577415886375 | 0.12074539088083652 | 15.89602165602948 | |||||||||||||||
| rep3 | 16 | 0.390462474098796 | 66.3959778515172 | 0.005880815174858872 | 0.774204006403195 | |||||||||||||||
| rep1 | 18 | 5.32533976218649 | 29.3346217813566 | 0.1815376997828201 | 23.89927421727764 | 0.04238813010100739 | 0.005379680245014963 | 0.003804008381865387 | ||||||||||||
| rep2 | 18 | 1.36951711563819 | 35.4943187668506 | 0.03858412171914201 | 5.079564776362002 | |||||||||||||||
| rep3 | 18 | 1.67732264295266 | 36.3118638374922 | 0.046192138482872784 | 6.081153311986127 | |||||||||||||||
| rep1 | 20 | 8.50976770141459 | 44.2896674839318 | 0.19213889344511148 | 25.2949117882542 | 0.181389891763515 | 0.01520138396008495 | 0.010749001681596482 | ||||||||||||
| rep2 | 20 | 0 | 0.156049777991922 | 0 | 0 | |||||||||||||||
| rep3 | 20 | 7.83818316283474 | 45.9337920651487 | 0.17064089008191852 | 22.464719061809134 | |||||||||||||||
| rep1 | 22 | 39.8393140613099 | 32.1880124564178 | 1.2377065566024579 | 162.94295031914882 | 1.056063792336563 | 0.15874838428123483 | 0.09165342239818909 | ||||||||||||
| rep2 | 22 | 41.6250656665072 | 42.191085754876 | 0.9865843678056238 | 129.88294096969426 | |||||||||||||||
| rep3 | 22 | 33.8578599103289 | 35.8701596307204 | 0.9439004526016075 | 124.26364208385287 | |||||||||||||||
| TIME NOT CONSIDERED | rep1 | 24 | 0.169295736982401 | 42.8658718907098 | 0.00394942945320312 | 0.5199388205139457 | 0.0029664380080523622 | 0.001241925837661127 | 7.170262166872032E-4 | |||||||||||
| ERROR HARVEST | rep2 | 24 | 0.16620618730255 | 49.1851208509591 | 0.0033791964811103843 | 0.4448681647543907 | ||||||||||||||
| rep3 | 24 | 0.119361969140528 | 75.9934260101347 | 0.0015706880898435813 | 0.20677966843191334 | |||||||||||||||
| LHY | ||||||||||||||||||||
| toc1-9 | ZT | GOI Conc | ACT Conc | GOI/UBQ | ratio/chosen sample | Average | SD | SE | ||||||||||||
| rep1 | 0 | 25.0732421822862 | 54.8932400150164 | 0.4567637504258676 | 60.13253520893761 | 0.6514320276726221 | 0.31677925668467305 | 0.18289258912058556 | ||||||||||||
| rep2 | 0 | 18.04330197056 | 37.5453517549814 | 0.4805735231436758 | 63.26707028300053 | |||||||||||||||
| rep3 | 0 | 28.9702152284569 | 28.4871077956172 | 1.0169588094483228 | 133.88170877871693 | |||||||||||||||
| rep1 | 2 | 9.29128823092551 | 37.7885248078337 | 0.24587591810409573 | 32.36934255102193 | 0.0672762043457649 | 0.011626657043174627 | 0.008221288037759112 | ||||||||||||
| rep2 | 2 | 2.1130557680963 | 35.7811999440569 | 0.059054916308005745 | 7.774526395409225 | |||||||||||||||
| rep3 | 2 | 3.40868323749272 | 45.1496219262059 | 0.07549749238352403 | 9.939176685334562 | |||||||||||||||
| rep1 | 4 | 3.13598581082793 | 48.9072585089985 | 0.06412107131809354 | 8.4414811268957 | 0.07340015055508498 | 0.013122599703287854 | 0.009279079236991417 | ||||||||||||
| rep2 | 4 | 1.50912731202079 | 15.5323828401016 | 0.09716006407751655 | 12.79103468389646 | |||||||||||||||
| rep3 | 4 | 3.80849441513868 | 46.0634965361478 | 0.08267922979207641 | 10.884645928851535 | |||||||||||||||
| rep1 | 6 | 0.406077769072031 | 63.8098505163217 | 0.006363872752971922 | 0.8377981002793934 | |||||||||||||||
| rep2 | 6 | 5.80459831579341 | 44.3757311770478 | 0.1308056940545847 | 17.22045148270458 | |||||||||||||||
| rep3 | 6 | 1.40950876066384 | 60.0792141228617 | 0.02346083884821465 | 3.0885982452770055 | |||||||||||||||
| rep1 | 8 | 1.30815258948381 | 18.309939980294 | 0.07144494143026706 | 9.405662012474616 | 0.051657401779550416 | 0.02271158531389645 | 0.013112539894701267 | ||||||||||||
| rep2 | 8 | 1.24897954366626 | 22.040089733409 | 0.0566685326046119 | 7.460361136163998 | |||||||||||||||
| rep3 | 8 | 0.827340539264676 | 30.8034109991069 | 0.026858731303772282 | 3.535927718181761 | |||||||||||||||
| rep1 | 10 | 0.58393567379962 | 22.7344090717165 | 0.025685104546046226 | 3.3814208155098355 | 0.026482956548317456 | 0.0011283331223785004 | 7.978520022712282E-4 | ||||||||||||
| rep2 | 10 | 0.806200294493142 | 29.5519208310175 | 0.027280808550588682 | 3.5914938065182698 | |||||||||||||||
| rep3 | 10 | 0.28899876121721 | 31.1968644274649 | 0.009263711803125416 | 1.2195592921888376 | |||||||||||||||
| rep1 | 12 | 1.33528907518191 | 50.6013597981068 | 0.026388403009514946 | 3.4740094230284795 | 0.014501845124493427 | 0.0081187989565625 | 0.00405939947828125 | ||||||||||||
| rep2 | 12 | 1.07312116291939 | 88.2091523864034 | 0.01216564419776469 | 1.601596071009192 | |||||||||||||||
| rep3 | 12 | 0.875611601792129 | 77.0201299901591 | 0.011368607166775832 | 1.4966668657382427 | |||||||||||||||
| rep4 | 12 | 0.875160047576666 | 108.248570719984 | 0.008084726123918242 | 1.0643468923439319 | |||||||||||||||
| rep1 | 14 | 0.409776707312507 | 92.7928965694846 | 0.004416035305091059 | 0.581367059491289 | 0.003814454775239107 | 0.0018106639951184606 | 0.0010453873449936064 | ||||||||||||
| rep2 | 14 | 0.681582239907772 | 129.880657190206 | 0.00524775786212428 | 0.6908625829391046 | |||||||||||||||
| rep3 | 14 | 0.265578135755995 | 149.237154404972 | 0.0017795711585019808 | 0.23427893576418543 | |||||||||||||||
| rep1 | 16 | 1.14102347129749 | 112.256551988193 | 0.010164426495279327 | 1.3381375679137695 | 0.06458061290082012 | 0.005679630393948799 | 0.0040161051661944175 | ||||||||||||
| rep2 | 16 | 5.57553289195232 | 81.2798782371102 | 0.06859671806701455 | 9.030695979128025 | |||||||||||||||
| rep3 | 16 | 3.91497870439338 | 64.6414682597201 | 0.06056450773462571 | 7.9732627433083305 | |||||||||||||||
| rep1 | 18 | 42.3481577427163 | 56.2757290957649 | 0.7525119340640096 | 99.06751647445786 | 0.5739433106477756 | 0.25253416904953213 | 0.1785686234162341 | ||||||||||||
| rep2 | 18 | 0.376244550252287 | 0.416911375439246 | 0.9024569067128149 | 118.80763669809818 | |||||||||||||||
| rep3 | 18 | 17.8600299813171 | 45.1724163384739 | 0.3953746872315417 | 52.05072048407226 | |||||||||||||||
| rep1 | 20 | 62.3666315831228 | 40.5352732622721 | 1.538576813818351 | 202.55224794508453 | 1.6013707841397113 | 0.07667323122536591 | 0.04426731068760343 | ||||||||||||
| rep2 | 20 | 94.8607181543324 | 60.0872168715312 | 1.5787171230970525 | 207.83668340816297 | |||||||||||||||
| rep3 | 20 | 76.5385677616078 | 45.3745151571346 | 1.6868184155037305 | 222.0681209198208 | |||||||||||||||
| rep1 | 22 | 0.0353668190056912 | 0.235310937534101 | 0.15029823677688536 | 19.786627127045037 | 0.004987056714385209 | 0.0035263816209036842 | |||||||||||||
| rep2 | 22 | 9.77929729687982 | 4.25923525648614 | 2.296021869650778 | 302.2692054449144 | |||||||||||||||
| rep3 | 22 | 29.3086706537185 | 12.8043102773454 | 2.2889691064089708 | 301.340715533964 | |||||||||||||||
| TIME NOT CONSIDERED | rep1 | 24 | 1.34547746647555 | 58.7459811154014 | 0.022903310846617335 | 3.0152001874084435 | 0.005156431597900259 | 0.002977067171105607 | ||||||||||||
| ERROR HARVEST | rep2 | 24 | 0.634143695478508 | 42.4132545271394 | 0.014951545278675373 | 1.9683574321730044 | ||||||||||||||
| rep3 | 24 | 1.32557784339139 | 53.8534118511504 | 0.02461455640090654 | 3.24048411908755 | |||||||||||||||
| 0.134241901056854 | 0.181957523232705 | |||||||||||||||||||
| LHY | ||||||||||||||||||||
| gi-11 | ZT | GOI Conc | ACT Conc | GOI/UBQ | ratio/chosen sample | Average | SD | SE | ||||||||||||
| rep1 | 0 | 166.188926353302 | 94.6865060365028 | 1.7551489996814766 | 231.06378061279042 | 1.609260049424785 | 0.2063181320533869 | 0.14588895025669146 | ||||||||||||
| rep2 | 0 | 149.732106505308 | 102.319983352431 | 1.4633710991680937 | 192.65148353481007 | |||||||||||||||
| rep3 | 0 | 0 | 0.580322759635 | 0 | 0 | |||||||||||||||
| rep1 | 2 | 216.014183501473 | 166.423110190679 | 1.2979818923824649 | 170.87814383580258 | 1.5237783694931526 | 0.2551782115108721 | 0.1473272091071293 | ||||||||||||
| rep2 | 2 | 164.264440175823 | 91.2265307467096 | 1.8006213634485686 | 237.05017623352185 | |||||||||||||||
| rep3 | 2 | 128.566080442913 | 87.2976843759519 | 1.4727318526484239 | 193.88381827615794 | |||||||||||||||
| rep1 | 4 | 92.9674129184223 | 192.155971444402 | 0.48381225011954043 | 63.69344576425695 | 0.4566242790513498 | 0.1340571846699325 | 0.07739795165598891 | ||||||||||||
| rep2 | 4 | 45.4458089887869 | 146.101206622176 | 0.3110570407971491 | 40.95037848402609 | |||||||||||||||
| rep3 | 4 | 57.7036128236166 | 100.353490341426 | 0.5750035462373599 | 75.69869753706246 | |||||||||||||||
| rep1 | 6 | 12.0081356042005 | 167.939337642059 | 0.07150281627163667 | 9.413281183070193 | 0.07326787628515338 | 0.002496171809517786 | 0.0017650600135167213 | ||||||||||||
| rep2 | 6 | 32.7050269390329 | 120.594255019256 | 0.27119888035968787 | 35.70308766106899 | |||||||||||||||
| rep3 | 6 | 12.7272489704363 | 169.622163256083 | 0.07503293629867011 | 9.878018296335963 | |||||||||||||||
| rep1 | 8 | 2.31208378161248 | 95.7636364766702 | 0.024143650624376053 | 3.1784897989145717 | 0.022948538635409046 | 0.0025865672031760416 | 0.0014933552710307452 | ||||||||||||
| rep2 | 8 | 2.89552879449968 | 144.917615869939 | 0.019980516358331247 | 2.630416932801472 | |||||||||||||||
| rep3 | 8 | 2.84186951397038 | 114.955621038322 | 0.02472144892351984 | 3.2545564231476325 | |||||||||||||||
| rep1 | 10 | 1.08585125710337 | 80.6568640602965 | 0.013462601971378682 | 1.7723393905340772 | 0.013498187188249022 | 5.032509631802472E-5 | 3.5585216870341434E-5 | ||||||||||||
| rep2 | 10 | 0 | 1.49164527846299 | 0 | 0 | |||||||||||||||
| rep3 | 10 | 0.980312663486483 | 72.4345462700159 | 0.013533772405119365 | 1.7817089138571447 | |||||||||||||||
| rep1 | 12 | 1.04108477667922 | 74.2787702464308 | 0.014015912934816603 | 1.8451822791376506 | 0.009851071596153331 | 0.004023077638494846 | 0.0023227249575557634 | ||||||||||||
| rep2 | 12 | 0.994457138716747 | 166.114004783894 | 0.005986594206855021 | 0.7881297204291992 | |||||||||||||||
| rep3 | 12 | 1.03321497332123 | 108.182033366781 | 0.00955070764678837 | 1.2573420358014622 | |||||||||||||||
| rep1 | 14 | 1.7657188307563 | 88.1722312892246 | 0.020025792757408487 | 2.6363775298477736 | 0.02901617103357374 | 0.012714314889017368 | 0.008990378276165266 | ||||||||||||
| rep2 | 14 | 2.95592802535499 | 77.7741752155739 | 0.038006549309739 | 5.0035278903092975 | |||||||||||||||
| rep3 | 14 | 8.04207663387694 | 101.026345227603 | 0.07960375697803267 | 10.479762710552821 | |||||||||||||||
| rep1 | 16 | 1.74094264109286 | 129.388622910908 | 0.01345514467907743 | 1.7713576447378088 | 0.01577924655222094 | 0.002168906811596775 | 0.001252218931522611 | ||||||||||||
| rep2 | 16 | 1.7451273273333 | 108.170142590519 | 0.016133170258816488 | 2.12391729360213 | |||||||||||||||
| rep3 | 16 | 1.46935955624617 | 82.7835031009436 | 0.0177494247187689 | 2.336695733504761 | |||||||||||||||
| rep1 | 18 | 6.25347861176787 | 130.15998446588 | 0.04804455560923296 | 6.325022353626661 | 0.056676081034892134 | 0.008225538841159872 | 0.004749017064173374 | ||||||||||||
| rep2 | 18 | 5.54069421139483 | 96.2605545026505 | 0.057559342349750006 | 7.577635434560134 | |||||||||||||||
| rep3 | 18 | 5.61827407271385 | 87.207313632887 | 0.06442434514569345 | 8.481406852391864 | |||||||||||||||
| rep1 | 20 | 25.0858123638608 | 93.9745686132594 | 0.2669425647176773 | 35.14274755096848 | 0.29162764244467254 | 0.11189951481273383 | 0.0646052149993204 | ||||||||||||
| rep2 | 20 | 32.3098180752019 | 78.0791290717282 | 0.41380863822802316 | 54.47753348379532 | |||||||||||||||
| rep3 | 20 | 15.1613285547539 | 78.0981501221668 | 0.19413172438831713 | 25.557266182065106 | |||||||||||||||
| rep1 | 22 | 23.7064550484866 | 44.7273539982383 | 0.5300214059034285 | 69.77683938855225 | 0.4827831065299017 | 0.06680504363748209 | 0.047238299373526804 | ||||||||||||
| rep2 | 22 | 0 | 0.536310798472776 | 0 | 0 | |||||||||||||||
| rep3 | 22 | 46.5409065590519 | 106.856759153926 | 0.4355448071563749 | 57.3390804917899 | |||||||||||||||
| TIME NOT CONSIDERED | rep1 | 24 | 0.824979750757634 | 71.3145832286428 | 0.011568177410679854 | 1.5229401081026508 | 0.007803836634568729 | 0.003347374869617409 | 0.0019326077820521997 | |||||||||||
| ERROR HARVEST | rep2 | 24 | 0.776156724857217 | 150.362175587834 | 0.005161914702436753 | 0.6795614084970788 | ||||||||||||||
| rep3 | 24 | 0.866419297519547 | 129.675964694178 | 0.006681417790589579 | 0.8796026176850851 | |||||||||||||||
| LHY | ||||||||||||||||||||
| COL-4 | ZT | GOI Conc | ACT Conc | GOI/UBQ | ratio/chosen sample | Average | SD | SE | SE | |||||||||||
| rep1 | 0 | 623.007527044159 | 76.2549122628976 | 8.170064177587257 | 460.70525440427207 | 8.120267540288646 | 0.18994053667567537 | 0.10966221997972317 | Time | Col-4 | prr7/9 | Col-4 | prr7/9 | |||||||
| rep2 | 0 | 626.310996226155 | 79.175750361357 | 7.910389145258245 | 446.0623275886219 | 0 | 8.120267540288646 | 3.9488868530481147 | 0.10966221997972317 | 0.22229499791061988 | ||||||||||
| rep3 | 0 | 276.707919944753 | 33.4174211721847 | 8.28034929802044 | 466.9241693799412 | 2 | 5.296323062516135 | 2.302288362180502 | 0.955710766072986 | 0.03763090164466442 | ||||||||||
| rep1 | 2 | 203.504891739054 | 31.1033666309978 | 6.542857374681742 | 368.94799181660727 | 5.296323062516135 | 1.6553396041789856 | 0.955710766072986 | 4 | 1.0532001529390247 | 1.4033388077238915 | 0.09882679475427783 | 0.029671486146926603 | |||||||
| rep2 | 2 | 271.877735506056 | 45.8636896739315 | 5.927951663701161 | 334.27380984517407 | 6 | 0.3001885733618888 | 1.1715953299927222 | 0.048284933123334074 | 0.051034723272266456 | ||||||||||
| rep3 | 2 | 142.922459511913 | 41.8126867305517 | 3.418160149165502 | 192.7480992665701 | 8 | 0.048296246328774904 | 1.1514253101966572 | 0.006981746933242767 | 0.09082789653160214 | ||||||||||
| rep1 | 4 | 76.154059048634 | 60.9555233860696 | 1.2493381209493113 | 70.44946335034594 | 1.0532001529390247 | 0.1711730296635906 | 0.09882679475427783 | 10 | 0.015031574166529768 | 0.9857150765655758 | 0.0010941795137301826 | 0.05967417177863843 | |||||||
| rep2 | 4 | 70.6429372106331 | 75.6369484759274 | 0.9339739192825353 | 52.666256070596205 | 12 | 0.016505389562942556 | 0.21007223006914066 | 7.933355381422376E-4 | 0.03723929286855507 | ||||||||||
| rep3 | 4 | 76.033505780426 | 77.8801677178643 | 0.9762884185852272 | 55.052346527475954 | 14 | 0.012807371636550319 | 0.056782917898465735 | 0.002356801157083524 | 0.00815624792411531 | ||||||||||
| rep1 | 6 | 14.420383324943 | 70.1835996406135 | 0.20546656767086488 | 11.586142443049054 | 0.3001885733618888 | 0.08363195740968 | 0.048284933123334074 | 16 | 0.04510496555622975 | 0.04395823060368467 | 0.007190445395783833 | 0.0035226540689816603 | |||||||
| rep2 | 6 | 13.6633398268737 | 41.2456566237495 | 0.3312673611069697 | 18.679977360923047 | 18 | 0.4182506852817565 | 1.1393922419489528 | 5.814584979589685E-4 | 0.731736188551206 | ||||||||||
| rep3 | 6 | 12.699270854732 | 34.9042364029903 | 0.363831791307832 | 20.516266987799504 | 20 | 2.073328158694886 | 1.5134185718293378 | 0.20636320223559051 | 0.27488668236631797 | ||||||||||
| rep1 | 8 | 2.49270909246736 | 40.1695627903304 | 0.0620546732230654 | 3.4992275939072304 | 0.048296246328774904 | 0.012092740413964667 | 0.006981746933242767 | 22 | 5.676149574577184 | 8.930208245685336 | 0.4584585701920375 | 2.065820604031529 | |||||||
| rep2 | 8 | 2.33107904655657 | 53.6103423657445 | 0.04348189068917538 | 2.45191899065977 | 24 | 8.1084653240152 | 8.250623428238336 | 0.6456935341744887 | 0.5202264721103629 | ||||||||||
| rep3 | 8 | 4.7981152583563 | 121.92757450696 | 0.03935217507408391 | 2.2190466849210564 | |||||||||||||||
| rep1 | 10 | 1.86946348372614 | 145.228306630365 | 0.01287258336272072 | 0.7258776264244481 | 0.015031574166529768 | 0.001895174510381684 | 0.0010941795137301826 | ||||||||||||
| rep2 | 10 | 1.94694339743325 | 118.567754577857 | 0.016420513354284686 | 0.9259433730138135 | |||||||||||||||
| rep3 | 10 | 1.68967376320053 | 106.930374535438 | 0.0158016257825839 | 0.8910446561897488 | |||||||||||||||
| Ref for Prrs | rep1 | 12 | 1.40876892549176 | 79.4396753939798 | 0.01773382026682502 | 1 | 0.016505389562942556 | 0.0013740974595123526 | 7.933355381422376E-4 | |||||||||||
| rep2 | 12 | 1.24565891122477 | 74.3194876840145 | 0.01676086515182882 | 0.9451356165588118 | |||||||||||||||
| rep3 | 12 | 1.36797086548109 | 91.0676290002126 | 0.015021483270173821 | 0.8470528653250637 | |||||||||||||||
| rep1 | 14 | 0.391023567742929 | 48.2352291635647 | 0.008106597076940919 | 0.45712638083436974 | 0.012807371636550319 | 0.004082099347405782 | 0.002356801157083524 | ||||||||||||
| rep2 | 14 | 0.653301336662272 | 42.2610402741768 | 0.015458714040729979 | 0.8717080588466815 | |||||||||||||||
| rep3 | 14 | 0.571597152569928 | 38.4737633055695 | 0.014856803791980056 | 0.83776668357088 | |||||||||||||||
| rep1 | 16 | 1.93354011103009 | 45.0312776446971 | 0.04293771378831363 | 2.421233165909434 | 0.04510496555622975 | 0.012454216754547302 | 0.007190445395783833 | ||||||||||||
| rep2 | 16 | 0.862911801034037 | 25.4721971932369 | 0.03387661435281083 | 1.9102829420338958 | |||||||||||||||
| rep3 | 16 | 0.780619016227422 | 13.3437851267993 | 0.05850056852756477 | 3.298813659288227 | |||||||||||||||
| rep1 | 18 | 4.39494070373931 | 10.5060655765655 | 0.4183241263544486 | 23.589058649535044 | 0.4182506852817565 | 0.0010071156609576177 | 5.814584979589685E-4 | ||||||||||||
| rep2 | 18 | 8.63314969638645 | 20.6926327232403 | 0.41720885939711244 | 23.526169382555015 | |||||||||||||||
| rep3 | 18 | 11.9533313503663 | 28.5133291949108 | 0.4192190700937087 | 23.63952401603784 | |||||||||||||||
| rep1 | 20 | 162.959398867107 | 67.3152805078545 | 2.420838146074316 | 136.509680917598 | 2.073328158694886 | 0.3574315510846541 | 0.20636320223559051 | ||||||||||||
| rep2 | 20 | 146.318173727807 | 69.928158275162 | 2.0924070837395154 | 117.9896408250973 | |||||||||||||||
| rep3 | 20 | 228.150356698214 | 133.676164766654 | 1.7067392462708275 | 96.24205166123487 | |||||||||||||||
| rep1 | 22 | 363.310155953279 | 68.9542343336598 | 5.268859258088089 | 297.10796539111425 | 5.676149574577184 | 0.7940735367379913 | 0.4584585701920375 | ||||||||||||
| rep2 | 22 | 302.312316421133 | 45.8658492939942 | 6.591229009700657 | 371.6756406982983 | |||||||||||||||
| rep3 | 22 | 170.004693566199 | 32.8933508054223 | 5.168360455942803 | 291.44089531635484 | |||||||||||||||
| rep1 | 24 | 316.04609106031 | 39.6361197379905 | 7.973688977364391 | 449.63176898104217 | 8.1084653240152 | 1.1183740073089257 | 0.6456935341744887 | ||||||||||||
| rep2 | 24 | 186.763324731095 | 26.4402953696931 | 7.063586927442967 | 398.31163399444995 | |||||||||||||||
| rep3 | 24 | 272.090820935712 | 29.2944986677607 | 9.288120067238237 | 523.7517876852339 | |||||||||||||||
| LHY | ||||||||||||||||||||
| prr9-1/7-3 | ZT | GOI Conc | ACT Conc | GOI/UBQ | ratio/chosen sample | Average | SD | SE | ||||||||||||
| rep1 | 0 | 67.4164467795073 | 21.7342234036472 | 3.101856713601012 | 174.9119291235691 | 3.9488868530481147 | 0.3143726008928975 | 0.22229499791061988 | ||||||||||||
| rep2 | 0 | 254.87952139262 | 68.3948045024684 | 3.7265918551374946 | 210.1403870720906 | |||||||||||||||
| rep3 | 0 | 219.854119854518 | 52.707872183512 | 4.171181850958734 | 235.21056310477218 | |||||||||||||||
| rep1 | 2 | 306.875708892294 | 132.843307448912 | 2.310057727298835 | 130.26283635118946 | 2.302288362180502 | 0.065178633583186 | 0.03763090164466442 | ||||||||||||
| rep2 | 2 | 361.163194742569 | 152.825823053376 | 2.36323408915278 | 133.2614210359245 | |||||||||||||||
| rep3 | 2 | 285.678388782175 | 127.901955403808 | 2.233573270089892 | 125.94992147677723 | |||||||||||||||
| rep1 | 4 | 162.566731565295 | 113.444217575139 | 1.4330102938708182 | 80.8066323166462 | 1.4033388077238915 | 0.041961818124749015 | 0.029671486146926603 | ||||||||||||
| rep2 | 4 | 116.333288377537 | 84.6881093771575 | 1.373667321576965 | 77.46031598993419 | |||||||||||||||
| rep3 | 4 | 0.0479170968305335 | 0.01590251486184 | 3.01317730225905 | 169.91134774811354 | |||||||||||||||
| rep1 | 6 | 54.4560352296058 | 47.4583497557068 | 1.1474489844236007 | 64.70399311366397 | 1.1715953299927222 | 0.08839473365778329 | 0.051034723272266456 | ||||||||||||
| rep2 | 6 | 78.9053503834715 | 62.1520164148447 | 1.2695541502113734 | 71.5894336984091 | |||||||||||||||
| rep3 | 6 | 68.5396545217762 | 62.4346191855484 | 1.0977828553431928 | 61.90334845091641 | |||||||||||||||
| rep1 | 8 | 68.8869677448182 | 70.1844168586059 | 0.9815137152681406 | 55.34699802412434 | 1.1514253101966572 | 0.1573185315373439 | 0.09082789653160214 | ||||||||||||
| rep2 | 8 | 78.9242587896555 | 66.8434153224035 | 1.1807334859983274 | 66.58088715419919 | |||||||||||||||
| rep3 | 8 | 32.3458603200532 | 25.0349389188809 | 1.2920287293235029 | 72.85676238303398 | |||||||||||||||
| rep1 | 10 | 6.2732981106343 | 20.2008403819414 | 0.31054639272543993 | 17.511533784200164 | 0.9857150765655758 | 0.08439202305273227 | 0.05967417177863843 | ||||||||||||
| rep2 | 10 | 5.93473210079312 | 5.6770548484147 | 1.0453892483442142 | 58.948902865551354 | |||||||||||||||
| rep3 | 10 | 3.122897458754 | 3.37231049148149 | 0.9260409047869373 | 52.21891791241951 | |||||||||||||||
| rep1 | 12 | 0.591095200993448 | 2.08602216658087 | 0.28335998076295266 | 15.978507535290595 | 0.21007223006914066 | 0.06450034728627474 | 0.03723929286855507 | ||||||||||||
| rep2 | 12 | 1.57851213034544 | 8.53635789375564 | 0.1849163483996054 | 10.42732731116779 | |||||||||||||||
| rep3 | 12 | 1.34403171771426 | 8.29954749416614 | 0.1619403610448639 | 9.131724502013176 | |||||||||||||||
| rep1 | 14 | 2.47526711339092 | 50.9034880384894 | 0.04862666997435045 | 2.7420301572198316 | 0.056782917898465735 | 0.011534676432361276 | 0.00815624792411531 | ||||||||||||
| rep2 | 14 | 0.00655546233937128 | 0.00557387744433756 | 1.1761044990379006 | 66.31986122234815 | |||||||||||||||
| rep3 | 14 | 1.40576789689093 | 21.6474584957189 | 0.06493916582258102 | 3.661882484738153 | |||||||||||||||
| rep1 | 16 | 0.02390727204317 | 0.591243506139997 | 0.04043557653470301 | 2.280139074734313 | 0.04395823060368467 | 0.004981785159902633 | 0.0035226540689816603 | ||||||||||||
| rep2 | 16 | 0.0249300734936555 | 0.525054949281667 | 0.04748088467266633 | 2.6774199782259935 | |||||||||||||||
| rep3 | 16 | 0.0124836169006893 | 0.0174213772295789 | 0.7165688875328399 | 40.40691045422053 | |||||||||||||||
| rep1 | 18 | 0.103547291985209 | 0.0553394894211139 | 1.871128430500159 | 105.51186390450302 | 1.1393922419489528 | 1.0348312419283119 | 0.731736188551206 | ||||||||||||
| rep2 | 18 | 0.0890344491522266 | 0.218405806586555 | 0.40765605339774663 | 22.987492106275386 |
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| This is one of many sheets from Andrew Millar's reworking (Nov-Dec 2010) of Aurora PinasFenandez's data (2009-2010). The readme is for all sheets. | ||||||||||
| Harvesting - ZT0 and ZT12 were harvested in a darkroom. ZT0 plants were taken before lights came on in the cabinets; ZT12 immediately after lights went out. Harvesting took 3-4 mins for all genotypes, always in same order. | ||||||||||
| Calibration standard curves were different for Col and Ws samples - so levels CANNOT be compared across accessions, but relative levels can (Andrew thinks?) | ||||||||||
| AJM manually edited the 14h to 18h timepoints in the prr7-9- mutant, as many values were omitted when the ratio's looked reasonably close among reps | ||||||||||
| For toc1-9, ignore LHY data, much lower than CCA1 in first 4 timepoints; LUX ditto | ||||||||||
| Mean | SE | |||||||||
| Time | WS-2 | toc1-9 | gi-11 | Col-4 | prr7/9 | WS-2 | toc1-9 | gi-11 | Col-4 | prr7/9 |
| 0 | 4.631160493784372 | 0.6514320276726221 | 1.609260049424785 | 8.120267540288646 | 3.9488868530481147 | 1.050215241974152 | 0.18289258912058556 | 0.14588895025669146 | 0.10966221997972317 | 0.22229499791061988 |
| 2 | 1.8950559588403362 | 0.0672762043457649 | 1.5237783694931526 | 5.296323062516135 | 2.302288362180502 | 0.5496823878886727 | 0.008221288037759112 | 0.1473272091071293 | 0.955710766072986 | 0.03763090164466442 |
| 4 | 0.3010540455877197 | 0.07340015055508498 | 0.4566242790513498 | 1.0532001529390247 | 1.4033388077238915 | 0.0289287247343889 | 0.009279079236991417 | 0.07739795165598891 | 0.09882679475427783 | 0.029671486146926603 |
| 6 | 0.04606019336454031 | 0.07326787628515338 | 0.3001885733618888 | 1.1715953299927222 | 0.007478250397870409 | 0.0017650600135167213 | 0.048284933123334074 | 0.051034723272266456 | ||
| 8 | 0.015348707551106408 | 0.051657401779550416 | 0.022948538635409046 | 0.048296246328774904 | 1.1514253101966572 | 0.0028597088907186988 | 0.013112539894701267 | 0.0014933552710307452 | 0.006981746933242767 | 0.09082789653160214 |
| 10 | 0.006243423509607333 | 0.026482956548317456 | 0.013498187188249022 | 0.015031574166529768 | 0.9857150765655758 | 3.2549730765303044E-4 | 7.978520022712282E-4 | 3.5585216870341434E-5 | 0.0010941795137301826 | 0.05967417177863843 |
| 12 | 0.004997095291601669 | 0.014501845124493427 | 0.009851071596153331 | 0.016505389562942556 | 0.21007223006914066 | 0.0014454244284333986 | 0.00405939947828125 | 0.0023227249575557634 | 7.933355381422376E-4 | 0.03723929286855507 |
| 14 | 0.0024388292135717263 | 0.003814454775239107 | 0.02901617103357374 | 0.012807371636550319 | 0.056782917898465735 | 9.287697145538574E-5 | 0.0010453873449936064 | 0.008990378276165266 | 0.002356801157083524 | 0.00815624792411531 |
| 16 | 0.06458061290082012 | 0.01577924655222094 | 0.04510496555622975 | 0.04395823060368467 | 0.0040161051661944175 | 0.001252218931522611 | 0.007190445395783833 | 0.0035226540689816603 | ||
| 18 | 0.04238813010100739 | 0.5739433106477756 | 0.056676081034892134 | 0.4182506852817565 | 1.1393922419489528 | 0.003804008381865387 | 0.1785686234162341 | 0.004749017064173374 | 5.814584979589685E-4 | 0.731736188551206 |
| 20 | 0.181389891763515 | 1.6013707841397113 | 0.29162764244467254 | 2.073328158694886 | 1.5134185718293378 | 0.010749001681596482 | 0.04426731068760343 | 0.0646052149993204 | 0.20636320223559051 | 0.27488668236631797 |
| 22 | 1.056063792336563 | 0.4827831065299017 | 5.676149574577184 | 8.930208245685336 | 0.09165342239818909 | 0.047238299373526804 | 0.4584585701920375 | 2.065820604031529 | ||
| 24 | 8.1084653240152 | 8.250623428238336 | 0.6456935341744887 | 0.5202264721103629 | ||||||
