Selected Cell
Cell:
Value:
Result sheet
Absorption 540 nm
OD at 750 nm
calibration curve
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| Method name: new_glycogen | ||||||||||||
| Application: SparkControl | V2.3 | |||||||||||
| Device: Spark 10M | Serial number: 1510002693 | |||||||||||
| Firmware: | ABS:V4.2.9|ABS_MEX:V4.1.7|MTP:V9.0.18|FLUOR:V4.2.12|FLUOR_MEM:V4.1.7|FLUOR_MEX:V4.1.7 | |||||||||||
| Date: | 2025-07-10 | |||||||||||
| Time: | 12:25 | |||||||||||
| System | TECAN-PC | |||||||||||
| User | Tecan-PC\Tecan | |||||||||||
| Plate | [GRE96ft] - Greiner 96 Flat Transparent Cat. No.: 655101/655161/655192 | |||||||||||
| Lid lifter | No lid | |||||||||||
| Humidity Cassette | No humidity cassette | |||||||||||
| Smooth mode | Not selected | |||||||||||
| List of actions in this measurement script: | ||||||||||||
| Plate | ||||||||||||
| Absorbance | Label 1 | |||||||||||
| Move plate | ||||||||||||
| Name | GRE96ft | |||||||||||
| Plate layout | ||||||||||||
| Plate area | A1-G12;H1-H4 | |||||||||||
| <> | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| A | None | None | None | None | None | None | None | None | None | None | None | None |
| B | None | None | None | None | None | None | None | None | None | None | None | None |
| C | None | None | None | None | None | None | None | None | None | None | None | None |
| D | None | None | None | None | None | None | None | None | None | None | None | None |
| E | None | None | None | None | None | None | None | None | None | None | None | None |
| F | None | None | None | None | None | None | None | None | None | None | None | None |
| G | None | None | None | None | None | None | None | None | None | None | None | None |
| H | None | None | None | None | None | None | None | None | None | None | None | None |
| Mode | Absorbance | |||||||||||
| Name | Label 1 | |||||||||||
| Measurement wavelength | 540 | nm | ||||||||||
| Number of flashes | 10 | |||||||||||
| Settle time | 50 | ms | ||||||||||
| Part of Plate | A1-G12;H1-H4 | |||||||||||
| Start Time | 2025-07-10 12:25:23 | |||||||||||
| Temperature | 28.2 | °C | ||||||||||
| <> | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| A | 0.0466 | 0.0799 | 0.1423 | 0.2668 | 0.5528 | 1.0762 | 1.8708 | 0.0682 | 0.0904 | 0.0926 | 0.0705 | 0.0859 |
| B | 0.0671 | 0.0761 | 0.075 | 0.0804 | 0.0569 | 0.0621 | 0.0679 | 0.0656 | 0.0654 | 0.066 | 0.0836 | 0.0843 |
| C | 0.085 | 0.0637 | 0.0606 | 0.0636 | 0.0777 | 0.0744 | 0.0736 | 0.0605 | 0.0636 | 0.0609 | 0.8905 | 0.9847 |
| D | 1.0137 | 0.7683 | 0.6866 | 0.6038 | 0.6724 | 0.7654 | 0.6253 | 0.0566 | 0.0581 | 0.0592 | 0.0599 | 0.0636 |
| E | 0.0628 | 0.5106 | 0.6386 | 0.5439 | 1.1216 | 0.9682 | 1.0087 | 1.1688 | 1.0119 | 1.1164 | 0.0767 | 0.0648 |
| F | 0.0672 | 1.3184 | 1.4323 | 1.4428 | 1.0405 | 0.9891 | 0.9984 | 0.9379 | 0.8841 | 0.9287 | 0.0592 | 0.0592 |
| G | 0.0581 | 0.0616 | 0.0631 | 0.0615 | 1.0063 | 1.0246 | 1.0709 | 1.4658 | 1.4794 | 1.4832 | 1.4713 | 1.3603 |
| H | 1.5255 | 0.0647 | 0.0668 | 0.0702 | ||||||||
| End Time | 2025-07-10 12:25:44 | |||||||||||
| Movement | Out | |||||||||||
| End Time | 2025-07-10 12:25:53 | |||||||||||
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| blank | WT GT | WT GS | ΔpirC GT | ΔglgC GS | ΔpirCΔglgC GS | ΔglgA1 GS | ΔglgA2 GS | ΔglnB GS | Δpgm GT | |||||||||||||||||||||
| 2 days +N | 0.0466 | 0.0466 | 0.0466 | 0.0682 | 0.0904 | 0.0926 | 0.0705 | 0.0859 | 0.0671 | 0.0761 | 0.075 | 0.0804 | 0.0569 | 0.0621 | 0.0679 | 0.0656 | 0.0654 | 0.066 | 0.0836 | 0.0843 | 0.085 | 0.0637 | 0.0606 | 0.0636 | 0.0777 | 0.0744 | 0.0736 | 0.0605 | 0.0636 | 0.0609 |
| 2 days -N | 0.0466 | 0.0466 | 0.0466 | 1.3184 | 1.4323 | 1.4428 | 1.0405 | 0.9891 | 0.9984 | 0.9379 | 0.8841 | 0.9287 | 0.0592 | 0.0592 | 0.0581 | 0.0616 | 0.0631 | 0.0615 | 1.0063 | 1.0246 | 1.0709 | 1.4658 | 1.4794 | 1.4832 | 1.4713 | 1.3603 | 1.5255 | 0.0647 | 0.0668 | 0.0702 |
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| WT GT | WT GS | ΔpirC GT | ΔglgC GS | ΔpirCΔglgC GS | ΔglgA1 GS | ΔglgA2 GS | ΔglnB GS | Δpgm GT | |||||||||||||||||||
| 2 days+N | 0.405 | 0.406 | 0.402 | 0.384 | 0.327 | 0.378 | 0.426 | 0.379 | 0.389 | 0.371 | 0.371 | 0.374 | 0.404 | 0.404 | 0.405 | 0.391 | 0.39 | 0.407 | 0.444 | 0.444 | 0.461 | 0.421 | 0.412 | 0.44 | 0.392 | 0.401 | 0.401 |
| 2 days -N | 1.002 | 1.038 | 1.053 | 1.023 | 0.939 | 0.999 | 0.966 | 0.816 | 0.849 | 0.456 | 0.435 | 0.444 | 0.453 | 0.456 | 0.447 | 0.87 | 0.81 | 0.786 | 1.251 | 1.236 | 1.215 | 1.095 | 1.071 | 1.149 | 0.405 | 0.396 | 0.393 |
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| glucose conc. [µg/ml] | A540 | ||||||||
| 0 | 0.0466 | ||||||||
| 2.0625 | 0.0799 | ||||||||
| 4.125 | 0.1423 | ||||||||
| 8.25 | 0.2668 | ||||||||
| 16.5 | 0.5528 | ||||||||
| 33 | 1.0762 | ||||||||
