HepG2 qPCR statistical significance, palmitate/no-glucose (Fig. S12)

Selected Cell

Cell:

Value:

Rules Analysis

p values	points
0.1<p	0
0.1> p >0.05	1
0.01< p<0.05	2
0.005 < p <0.01	3
<0.005	4

Negative if down regulated
Positive if up regulated

Summary	Significance score versus WT * (using one-way Brown-Forsythe ANOVA)									p values										Points
Experiment number	KO4			KO9			KO21			Experiment number	Exp. 1			Exp. 2			Exp. 3			Experiment number	Exp. 1			Exp. 2			Exp. 3
GOI	Exp. 1	Exp. 2	Exp. 3	Exp. 1	Exp. 2	Exp. 3	Exp. 1	Exp. 2	Exp. 3	GOI	KO4	KO9	KO21	KO4	KO9	KO21	KO4	KO9	KO21	GOI	KO4	KO9	KO21	KO4	KO9	KO21	KO4	KO9	KO21
Acot1	0	-2	0	0	0	0	0	-2	-1	Acot1	0.5435	0.3019	0.9055	0.0147	0.7291	0.0492	0.8407	0.9791	0.0689	Acot1	0	0	0	-2	0	-2	0	0	-1
Acot2	0	-4	0	0	4	0	0	-2	-2	Acot2	0.998	0.5654	0.5773	0.0031	0.0036	0.0415	0.9982	0.2904	0.0296	Acot2	0	0	0	-4	4	-2	0	0	-2
Acot4	0	-1	0	-2	0	0	-1	0	0	Acot4	0.2803	0.0259	0.0749	0.0752	0.1247	0.9934	0.9981	0.3458	0.262	Acot4	0	-2	-1	-1	0	0	0	0	0
Acot6	0	-2	0	-1	-2	0	-1	0	0	Acot6	0.5993	0.0654	0.0885	0.0389	0.0405	0.9923	0.4321	0.2605	0.6885	Acot6	0	-1	-1	-2	-2	0	0	0	0
Acot7	4	0	4	0	0	0	2	0	0	Acot7	0.0016	0.9924	0.0175	0.8545	0.9518	0.3169	0.0044	0.7343	0.2382	Acot7	4	0	2	0	0	0	4	0	0
Acot8	0	0	0	4	4	4	-2	-4	-4	Acot8	0.1129	<0,0001	0.0237	0.995	<0,0001	3.0E-4	0.8495	<0,0001	0.0026	Acot8	0	4	-2	0	4	-4	0	4	-4
Acot9	2	0	0	-4	0	0	0	3	0	Acot9	0.0297	7.0E-4	0.184	0.1575	0.8679	0.0069	0.9993	0.2989	0.2485	Acot9	2	-4	0	0	0	3	0	0	0
Acot11	4	4	1	4	2	2	4	-4	-4	Acot11	<0,0001	<0,0001	0.0036	0.0039	0.0109	0.002	0.0703	0.0361	0.0025	Acot11	4	4	4	4	2	-4	1	2	-4
Acot12	0	0	0	0	0	0	0	0	0	Acot12	0.6416	0.7381	0.3528	0.8992	0.9993	0.958	0.7216	0.4447	0.6264	Acot12	0	0	0	0	0	0	0	0	0
Acot13	0	1	4	4	4	4	0	-4	-1	Acot13	0.3765	0.0013	0.9767	0.0572	0.0019	1.0E-4	<0,0001	0.0011	0.0671	Acot13	0	4	0	1	4	-4	4	4	-1
Nudt7	0	0	0	2	4	0	0	-4	-4	Nudt7	0.9518	0.0151	0.4912	0.998	<0,0001	3.0E-4	0.2748	0.1663	0.0037	Nudt7	0	2	0	0	4	-4	0	0	-4
Nudt8	0	0	0	2	0	0	0	0	-3	Nudt8	0.9976	0.0239	0.8657	0.9388	0.369	0.6807	0.6005	0.9778	0.0083	Nudt8	0	2	0	0	0	0	0	0	-3
Nudt19	-4	-4	-3	-4	-3	-4	-4	4	1	Nudt19	<0,0001	<0,0001	0.0028	1.0E-4	0.006	0.002	0.0081	<0,0001	0.0997	Nudt19	-4	-4	-4	-4	-3	4	-3	-4	1
SLC25A42	-4	-4	0	0	0	0	-2	-4	0	SLC25A42	0.0043	0.1706	0.0148	0.0044	0.9605	0.0045	0.9245	>0,9999	0.2278	SLC25A42	-4	0	-2	-4	0	-4	0	0	0
SLC25A16	0	0	4	0	0	0	-1	0	-4	SLC25A16	0.9805	0.8167	0.0978	0.2764	0.5606	0.8989	0.0038	0.9756	0.0012	SLC25A16	0	0	-1	0	0	0	4	0	-4
SLC25A17	-2	0	0	0	4	4	-3	-4	-3	SLC25A17	0.0138	0.9396	0.0097	0.9998	0.004	0.0035	>0,9999	<0,0001	0.0076	SLC25A17	-2	0	-3	0	4	-4	0	4	-3
pexCRAT	0	4	4	2	4	2	0	-4	0	pexCRAT	0.4544	0.0377	0.5859	2.0E-4	<0,0001	4.0E-4	1.0E-4	0.0336	0.9682	pexCRAT	0	2	0	4	4	-4	4	2	0
pexCROT	0	0	4	0	0	0	-2	0	0	pexCROT	0.9428	0.8512	0.0434	0.9227	0.7103	0.6235	<0,0001	0.5462	0.9556	pexCROT	0	0	-2	0	0	0	4	0	0
CPT1a	0	4	4	2	4	4	0	2	0	CPT1a	0.7691	0.0102	0.5006	0.0018	<0,0001	0.0212	3.0E-4	0.0048	0.2671	CPT1a	0	2	0	4	4	2	4	4	0
CPT2	-2	0	4	0	4	4	0	-1	-4	CPT2	0.0223	0.1172	0.2381	0.8804	0.0048	0.0662	1.0E-4	0.0012	0.001	CPT2	-2	0	0	0	4	-1	4	4	-4
Pank1	0	2	4	4	1	0	-1	-2	-3	Pank1	0.9706	8.0E-4	0.0821	0.0219	0.0868	0.022	<0,0001	0.1666	0.0095	Pank1	0	4	-1	2	1	-2	4	0	-3
Pank2	0	-1	0	0	0	-1	0	0	0	Pank2	0.6454	>0,9999	0.1467	0.0762	0.9931	0.8735	0.9784	0.059	0.3162	Pank2	0	0	0	-1	0	0	0	-1	0
Pank3	-2	-4	-4	0	0	0	-2	-4	-4	Pank3	0.0154	0.8586	0.0404	0.004	0.9602	0.0025	6.0E-4	0.7269	0.0029	Pank3	-2	0	-2	-4	0	-4	-4	0	-4
Pank4	0	-2	-4	0	0	0	2	0	0	Pank4	0.4678	0.9213	0.0412	0.0415	0.4057	0.2537	0.0015	0.9977	0.8978	Pank4	0	0	2	-2	0	0	-4	0	0
PPCS	2	0	1	4	2	4	2	-2	1	PPCS	0.0383	8.0E-4	0.0188	0.4986	0.0336	0.0193	0.0596	0.0012	0.0554	PPCS	2	4	2	0	2	-2	1	4	1
PPCDC	0	0	0	0	0	2	-2	0	0	PPCDC	0.2678	0.9339	0.0354	0.1057	0.7135	0.2797	0.3121	0.0295	0.9097	PPCDC	0	0	-2	0	0	0	0	2	0
Coasy	-4	-4	0	2	0	-1	-4	0	0	Coasy	<0,0001	0.0228	0.005	<0,0001	0.2263	0.9981	0.989	0.0994	0.3254	Coasy	-4	2	-4	-4	0	0	0	-1	0
ABCD3	0	0	2	2	0	0	0	0	0	ABCD3	0.1047	0.0169	0.3278	0.1056	0.3022	0.3702	0.0183	0.9221	0.457	ABCD3	0	2	0	0	0	0	2	0	0

																				0.1<p	0
																				0.1> p >0.05	1
																				0.01< p<0.05	2
																				0.005 < p <0.01	3
																				p<0.005	4

																				Negative if down regulated
																				Positive if up regulated