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| Datafile metadata | How to use this sheet Details on how to use this sheet and what the cells mean can be found by visiting: | ||||||||
| Data File Title: | samples-250609-SC-R719C-DNA.xlsx | ||||||||
| Data File Description: | Batch sample publishing | ||||||||
| Project SEEK ID: | https://fairdomhub.org/projects/485 | ||||||||
| Master metadata sheet guide | |||||||||
| Associated Assay | |||||||||
| Existing Assay SEEK ID: | https://fairdomhub.org/assays/2669 | ||||||||
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| New Assay metadata | |||||||||
| Study SEEK ID: | |||||||||
| Assay Title: | |||||||||
| Assay Description: | |||||||||
| Assay type: | Experimental_assay_type | Powered by: | |||||||
| Technology type: | Technology_type | ||||||||
| SOP SEEK ID: | |||||||||
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| UID | Name | Protocol | Scientist | Parent | Type | NumPrepCycles | Barcode | LibraryPrepProtocol | QC_Instrument | VolumeUnits | ExtractionMethod |
| DNA-250605SHO-1 | D21-157037 390 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-1 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-2 | D21-157038 391 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-2 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-3 | D21-157039 675 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-3 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-4 | D21-157040 676 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-4 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-5 | D21-157041 972 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-5 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-6 | D21-157042 973 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-6 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-7 | D21-157085 406 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-7 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-8 | D21-157086 407 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-8 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-9 | D21-157087 408 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-9 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-10 | D21-157088 409 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-10 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-11 | D21-157089 691 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-11 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-12 | D21-157090 692 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-12 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-13 | D21-157091 693 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-13 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-14 | D21-157092 694 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-14 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-15 | D21-157093 988 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-15 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-16 | D21-157094 989 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-16 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-17 | D21-157095 990 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-17 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
| DNA-250605SHO-18 | D21-157096 991 | https://fairdomhub.org/sops/833 | MIT BioMicroCenter | RNA-250605SHO-18 | cDNA | 8 | 6 bp sample barcode + UMI (IDT) | Oligo(dT)-primed, UMI-based 3′ RNA-Seq (3′DGE) library preparation | Fragment Analyzer | ng | TRIzol (Invitrogen) + Direct-zol RNA Microprep kit (Zymo Research) |
