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| UID | Name | Scientist | Strain | Genotype | Source | Protocol | Treatment1 | Treatment1Date | Treatment2 | Treatment2Date |
| MUS-250515FRI-1 | Balb/c_IntravitalMicroscopy | Peter Friedl | CANN.CG-FOXN1NU/CRL | Foxn1 | Charles River Laboritories | https://fairdomhub.org/sops/830 | Titanium dorsal skin imaging windows | 10 to 14 weeks after birth | HT-1080 tumor cells | 1 day post surgery (Treatment1) |
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| UID | File_PrimaryData | Scientist | Contact | Type | Protocol | Parent | Instrument | ObjectiveLens | ImageTimepoint | TimeInterval | TotalTime | Markers | ExcitationWavelengths | FluorescentLabels |
| D.IMG-250515FRI-1 | Raw Intravital Microscopy Images can be aquired by reaching out to the corresponding author | Peter Friedl | Corresponding Author: caterina.laporta@unimi.it | Intravital multiphoton microscopy (MPM) | https://fairdomhub.org/sops/830 | MUS-250515FRI-1 | LaVision BioTec multiphoton microscope | 20x magnification, NA 0.95 (Olympus) | 7 days post-surgery | 6 Minuetes | Approximately 4 hours | H2B-EGFP, DsRed2 | 910 nm for eGFP, 1090 nm for DsRed2 and SHG | Nuclear Marker: H2B-EGFP (green), Cytoplasmic Marker: DsRed2 (red) |
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| UID | File_PrimaryData | Link_PrimaryData | DataType | Scientist | Protocol | SourceCode | Parent | Description | Algorithm | Software |
| A.IMG-250515FRI-1 | MovieS1_IntravitalMicroscopy | https://www.pnas.org/doi/suppl/10.1073/pnas.2414009121/suppl_file/pnas.2414009121.sm01.avi | avi | Peter Friedl | https://fairdomhub.org/sops/831 | https://github.com/joshichaitanya3/actnempy | D.IMG-250515FRI-1 | Time-lapse of collectively invading HT1080 cells in the mouse dermis (position P1). Colors are: cytoplasm in green, nuclei in blue and collagen in magenta. | 3D Horn–Schunck Optical Flow | ImageJ with OrientationJ Plugin |
| A.IMG-250515FRI-2 | MovieS2_IntravitalMicroscopy | https://www.pnas.org/doi/suppl/10.1073/pnas.2414009121/suppl_file/pnas.2414009121.sm02.avi | avi | Peter Friedl | https://fairdomhub.org/sops/831 | https://github.com/joshichaitanya3/actnempy | D.IMG-250515FRI-1 | Time-lapse of collectively invading HT1080 cells in the mouse dermis (position P2). Colors are: cytoplasm in green, nuclei in blue and collagen in magenta. | 3D Horn–Schunck Optical Flow | ImageJ with OrientationJ Plugin |
| A.IMG-250515FRI-3 | MovieS3_IntravitalMicroscopy | https://www.pnas.org/doi/suppl/10.1073/pnas.2414009121/suppl_file/pnas.2414009121.sm03.avi | avi | Peter Friedl | https://fairdomhub.org/sops/831 | https://github.com/joshichaitanya3/actnempy | D.IMG-250515FRI-1 | Time-lapse of collectively invading HT1080 cells in the mouse dermis (position P3). Colors are: cytoplasm in green, nuclei in blue and collagen in magenta. | 3D Horn–Schunck Optical Flow | ImageJ with OrientationJ Plugin |
| A.IMG-250515FRI-4 | MovieS4_IntravitalMicroscopy | https://www.pnas.org/doi/suppl/10.1073/pnas.2414009121/suppl_file/pnas.2414009121.sm04.avi | avi | Peter Friedl | https://fairdomhub.org/sops/831 | https://github.com/joshichaitanya3/actnempy | D.IMG-250515FRI-1 | Time-lapse of collectively invading HT1080 cells in the mouse dermis (position P4). Colors are: cytoplasm in green, nuclei in blue and collagen in magenta. | 3D Horn–Schunck Optical Flow | ImageJ with OrientationJ Plugin |
| A.IMG-250515FRI-5 | MovieS5_IntravitalMicroscopy | https://www.pnas.org/doi/suppl/10.1073/pnas.2414009121/suppl_file/pnas.2414009121.sm05.mov | mov | Peter Friedl | https://fairdomhub.org/sops/831 | https://github.com/joshichaitanya3/actnempy | D.IMG-250515FRI-1 | Time-lapse of stream lines and topological defects obtained from position P4 as illustrated in Fig. 4a. | 3D Horn–Schunck Optical Flow | ImageJ with OrientationJ Plugin |
