Selected Cell
Cell:
Value:
Note on data sheet
Samples IDs
standards
standards2
protein_det
Analysis
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| The sheet "Samples IDs" contains the experimental details per animal as well as an identifier for each animal. | |||||||||
| The sheets "standards" and "standards2" contain standard curves over different relevant ranges of metabolite concentrations. | |||||||||
| The sheet "protein_det" contains the determined protein concentrations for the normalisation of the data | |||||||||
| The data used for plotting, were: | |||||||||
| Fig. 4C: "Analysis" contains the final calculations yielding the values plotted. | |||||||||
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| Group | Dilution 1:10 | |||||||||
| Experiment ID | Date Experiment | Tube ID | Animal ID | Genotype | Diet/treatment | Tissue Type | Samp weight (mg) | H2O Volume (uL)** | Tissue/ vol (mg/ µL) | Tissue/ vol (mg/ µL) |
| LK_01_AS | 21-09-22 | 1 | 16 | wt | FED | Liver | 82.6 | 413 | 0.19999999999999998 | 0.019999999999999997 |
| LK_01_AS | 21-09-22 | 2 | 14 | wt | FED | Liver | 91 | 455 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 3 | 13 | wt | FED | Liver | 87.6 | 438 | 0.19999999999999998 | 0.019999999999999997 |
| LK_01_AS | 21-09-22 | 4 | 20 | MCAD KO | FED | Liver | 82.6 | 413 | 0.19999999999999998 | 0.019999999999999997 |
| LK_01_AS | 21-09-22 | 5 | 17 | wt | FED | Liver | 89.8 | 449 | 0.19999999999999998 | 0.019999999999999997 |
| LK_01_AS | 21-09-22 | 6 | 18 | MCAD KO | FED | Liver | 82.7 | 413.5 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 7 | 22 | MCAD KO | FED | Liver | 85 | 425 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 8 | 21 | MCAD KO | FED | Liver | 95.4 | 477 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 9 | 19 | MCAD KO | FED | Liver | 93.7 | 468.5 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 10 | 15 | wt | FED | Liver | 91.2 | 456 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 11 | KO17-13 | MCAD KO | COLD | Liver | 51 | 255 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 12 | KO17-12* | MCAD KO | COLD | Liver | 22.5 | 112.5 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 13 | WT17-12 | wt | COLD | Liver | 49.8 | 249 | 0.19999999999999998 | 0.019999999999999997 |
| LK_01_AS | 21-09-22 | 14 | WT17-11 | wt | COLD | Liver | 47.5 | 237.5 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 15 | KO17-14 | MCAD KO | COLD | Liver | 40.2 | 201 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 16 | KO17-16 | MCAD KO | COLD | Liver | 73.8 | 369 | 0.19999999999999998 | 0.019999999999999997 |
| LK_01_AS | 21-09-22 | 17 | KO17-15 | MCAD KO | COLD | Liver | 45.6 | 228 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 18 | KO17-10 | MCAD KO | COLD | Liver | 60.2 | 301 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 19 | WT17-16 | wt | COLD | Liver | 46.3 | 231.5 | 0.19999999999999998 | 0.019999999999999997 |
| LK_01_AS | 21-09-22 | 20 | KO17-9 | MCAD KO | COLD | Liver | 56.4 | 282 | 0.19999999999999998 | 0.019999999999999997 |
| LK_01_AS | 21-09-22 | 21 | KO17-11 | MCAD KO | COLD | Liver | 56.9 | 284.5 | 0.19999999999999998 | 0.019999999999999997 |
| LK_01_AS | 21-09-22 | 22 | WT17-13 | wt | COLD | Liver | 45.7 | 228.5 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 23 | WT17-15 | wt | COLD | Liver | 31.5 | 157.5 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 24 | WT17-9* | wt | COLD | Liver | 21.6 | 108 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 25 | WT17-10 | wt | COLD | Liver | 40.7 | 203.5 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 26 | WT17-14 | wt | COLD | Liver | 34.3 | 171.5 | 0.19999999999999998 | 0.019999999999999997 |
| LK_01_AS | 21-09-22 | 27 | 89 (38) | wt | FASTED | Liver | 83.4 | 417 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 28 | 92 (41) | MCAD KO | FASTED | Liver | 94.7 | 473.5 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 29 | 90 (39) | wt | FASTED | Liver | 90 | 450 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 30 | 91 (40) | MCAD KO | FASTED | Liver | 94.3 | 471.5 | 0.19999999999999998 | 0.019999999999999997 |
| LK_01_AS | 21-09-22 | 31 | 88 (37) | MCAD KO | FASTED | Liver | 85 | 425 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 32 | 84 (33) | wt | FASTED | Liver | 84 | 420 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 33 | 83 (32) | wt | FASTED | Liver | 86.7 | 433.5 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 34 | 86 (35) | MCAD KO | FASTED | Liver | 75.6 | 378 | 0.19999999999999998 | 0.019999999999999997 |
| LK_01_AS | 21-09-22 | 35 | 85 (34) | MCAD KO | FASTED | Liver | 72.5 | 362.5 | 0.2 | 0.02 |
| LK_01_AS | 21-09-22 | 36 | 87 (36) | wt | FASTED | Liver | 87.6 | 438 | 0.19999999999999998 | 0.019999999999999997 |
| *Samples finished (from Anne-Claire's experiments) | ||||||||||
| ** Volume of MilliQ H2O to be added to the tissue to have 0.2 mg liver/ uL MilliQ H2O |
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| pmol/ul | |||||||||||||||||
| CoA | ret time | peak area | pPanSH | ret time | peak area | dephospho | ret time | peak area | pan | ret time | peak area | ||||||
| 0.2 | 17.255 | 378861 | 0.2 | 18.163 | 1132271 | 0.2 | 18.374 | 1645174 | 0.2 | 21.513 | 30760286 | ||||||
| 0.4 | 17.251 | 1954908 | 0.4 | 18.166 | 5397389 | 0.4 | 18.378 | 3825591 | 0.4 | 21.516 | 36439443 | ||||||
| 0.8 | 17.241 | 6984714 | 0.8 | 18.16 | 18622387 | 0.8 | 18.373 | 7902759 | 0.8 | 21.513 | 51287934 | ||||||
| 1.6 | 17.234 | 14693349 | 1.6 | 18.157 | 40748276 | 1.6 | 18.368 | 16647332 | 1.6 | 21.507 | 76572731 | ||||||
| 3.2 | 17.225 | 32688067 | 3.2 | 18.151 | 90427672 | 3.2 | 18.361 | 35486659 | |||||||||
| a | 1.07944900269E7 | a | 2.99063168011E7 | a | 1.12811155578E7 | a | 3.30254397391E7 | ||||||||||
| b | -2045187.8333 | b | -5818233.8333 | b | -887080.2917 | b | 2.39960186957E7 | ||||||||||
| pmol/ul | |||||||||||||||||
| liver | |||||||||||||||||
| CoA | ret time | peak area | pPanSH | ret time | peak area | dephospho | ret time | peak area | pan | ret time | peak area | ||||||
| 0.2 | 17.233 | 3423019 | 0.2 | 18.151 | 32486580 | 0.2 | 18.374 | 75998179 | 0.2 | 21.517 | 9777761 | ||||||
| 0.4 | 17.236 | 3611589 | 0.4 | 18.15 | 36474588 | 0.4 | 18.374 | 78698464 | 0.4 | 21.516 | 11743598 | ||||||
| 0.8 | 17.256 | 4597629 | 0.8 | 18.159 | 48340812 | 0.8 | 18.381 | 84059804 | 0.8 | 21.519 | 19628751 | ||||||
| 1.6 | 17.267 | 6371450 | 1.6 | 18.164 | 68114324 | 1.6 | 18.379 | 98910799 | 1.6 | 21.521 | 39772994 | ||||||
| 3.2 | 17.247 | 11805204 | 3.2 | 18.128 | 116620719 | to high | 3.2 | 18.335 | 116416795 | to high | 3.2 | 21.501 | 99104252 | ||||
| a | 2825149.6237 | a | 2.58091227826E7 | a | 1.64640943478E7 | a | 3.02238688777E7 | ||||||||||
| b | 2458592.6667 | b | 2.6997233913E7 | b | 7.20687407391E7 | b | -1472126.2083 | ||||||||||
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| pmol/ul | |||||||||||||||||
| CoA | ret time | peak area | pPanSH | ret time | peak area | dephospho | ret time | peak area | pan | ret time | peak area | ||||||
| 0.2 | 17.255 | 378861 | 0.2 | 18.163 | 1132271 | 0.2 | 18.374 | 1645174 | 0.2 | 21.513 | 30760286 | ||||||
| 0.4 | 17.251 | 1954908 | 0.4 | 18.166 | 5397389 | 0.4 | 18.378 | 3825591 | 0.4 | 21.516 | 36439443 | ||||||
| 0.8 | 17.241 | 6984714 | 0.8 | 18.16 | 18622387 | 0.8 | 18.373 | 7902759 | 0.8 | 21.513 | 51287934 | ||||||
| 1.6 | 17.234 | 14693349 | 1.6 | 18.157 | 40748276 | 1.6 | 18.368 | 16647332 | 1.6 | 21.507 | 76572731 | ||||||
| 3.2 | 17.225 | 32688067 | 3.2 | 18.151 | 90427672 | 3.2 | 18.361 | 35486659 | |||||||||
| a | 1.07944900269E7 | a | 2.99063168011E7 | a | 1.12811155578E7 | a | 3.30254397391E7 | ||||||||||
| b | -2045187.8333 | b | -5818233.8333 | b | -887080.2917 | b | 2.39960186957E7 | ||||||||||
| pmol/ul | |||||||||||||||||
| liver | |||||||||||||||||
| CoA | ret time | peak area | pPanSH | ret time | peak area | dephospho | ret time | peak area | pan | ret time | peak area | ||||||
| 0.2 | 17.233 | 3423019 | 0.2 | 18.151 | 32486580 | 0.2 | 18.374 | 75998179 | 0.2 | 21.517 | 9777761 | ||||||
| 0.4 | 17.236 | 3611589 | 0.4 | 18.15 | 36474588 | 0.4 | 18.374 | 78698464 | 0.4 | 21.516 | 11743598 | ||||||
| 0.8 | 17.256 | 4597629 | 0.8 | 18.159 | 48340812 | 0.8 | 18.381 | 84059804 | 0.8 | 21.519 | 19628751 | ||||||
| 1.6 | 17.267 | 6371450 | 1.6 | 18.164 | 68114324 | 1.6 | 18.379 | 98910799 | 1.6 | 21.521 | 39772994 | ||||||
| 3.2 | 17.247 | 11805204 | 3.2 | 18.128 | 116620719 | to high | 3.2 | 18.335 | 116416795 | to high | 3.2 | 21.501 | 99104252 | ||||
| a | 2825149.6237 | a | 2.81255227621E7 | a | 1.37186955914E7 | a | 3.02238688777E7 | ||||||||||
| b | 2458592.6667 | b | 2.5531756375E7 | b | 7.38056256667E7 | b | -1472126.2083 | ||||||||||
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| Sample ID | Group | Protein (µg/ µL) | Protein (mg/ µL) | ||||||
| 1 | M-1 | 20.454187036744994 | 0.020454187036744995 | ||||||
| 2 | M-2 | 20.966724491405138 | 0.020966724491405138 | ||||||
| 3 | M-3 | 19.902223624034065 | 0.019902223624034064 | ||||||
| 4 | M-4 | 21.676391736319193 | 0.021676391736319193 | ||||||
| 5 | M-5 | 22.464910897334807 | 0.02246491089733481 | ||||||
| 6 | M-6 | 21.912947484623874 | 0.021912947484623874 | ||||||
| 7 | M-7 | 23.45055984860432 | 0.02345055984860432 | ||||||
| 8 | M-8 | 21.282132155811382 | 0.021282132155811383 | ||||||
| 9 | M-9 | 21.63696577826841 | 0.021636965778268413 | ||||||
| 10 | M-10 | 22.93802239394417 | 0.02293802239394417 | ||||||
| 11 | M-11 | 15.56536823844819 | 0.01556536823844819 | ||||||
| 12 | M-12 | 16.945276770225515 | 0.016945276770225514 | ||||||
| 13 | M-13 | 25.14587604478789 | 0.02514587604478789 | ||||||
| 14 | M-14 | 18.719444882510643 | 0.018719444882510643 | ||||||
| 15 | M-15 | 18.798296798612206 | 0.018798296798612207 | ||||||
| 16 | M-16 | 20.21763128844031 | 0.02021763128844031 | ||||||
| 17 | M-17 | 18.91657467276455 | 0.01891657467276455 | ||||||
| 18 | M-18 | 16.945276770225515 | 0.016945276770225514 | ||||||
| 19 | M-19 | 22.228355149030122 | 0.022228355149030123 | ||||||
| 20 | M-20 | 21.479261946065286 | 0.021479261946065285 | ||||||
| 21 | M-21 | 20.375335120643427 | 0.020375335120643427 | ||||||
| 22 | M-22 | 22.93802239394417 | 0.02293802239394417 | ||||||
| 23 | M-23 | 21.794669610471534 | 0.021794669610471534 | ||||||
| 24 | M-24 | 20.966724491405138 | 0.020966724491405138 | ||||||
| 25 | M-25 | 18.601167008358303 | 0.018601167008358302 | ||||||
| 26 | M-26 | 21.282132155811382 | 0.021282132155811383 | ||||||
| 27 | M-27 | 26.486358618514426 | 0.026486358618514425 | ||||||
| 28 | M-28 | 28.457656521053458 | 0.02845765652105346 | ||||||
| 29 | M-29 | 26.131524996057404 | 0.026131524996057403 | ||||||
| 30 | M-30 | 27.432581611733163 | 0.02743258161173316 | ||||||
| 31 | M-31 | 26.60463649266677 | 0.02660463649266677 | ||||||
| 32 | M-32 | 29.837565052830783 | 0.02983756505283078 | ||||||
| 33 | M-33 | 30.310676549440153 | 0.03031067654944015 | ||||||
| 34 | M-34 | 24.59391263207696 | 0.024593912632076963 | ||||||
| 35 | M-35 | 25.54013562529569 | 0.02554013562529569 | ||||||
| 36 | M-36 | 29.12789780791673 | 0.029127897807916733 |
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| (Used Standards2 sheet, as we considered the last point in the std curve) | (Used Standards2 sheet, as we considered the last point in the std curve) | |||||||||||||||||||||||||||||||
| CoA | Diluted 10X | Adjust dilution | per mg liver | pPanSH | Diluted 10X | Adjust dilution | per mg liver | dephosphoCoA | Diluted 10X | Adjust dilution | per mg liver | pantetheine | ||||||||||||||||||||
| Group | ID | ret time | peak area | pmol/µl | pmol/µl | pmol/mg liver | Protein (mg/ µL) | pmol/mg protein | ret time | peak area | pmol/µl | pmol/µl | pmol/mg liver | pmol/mg protein | ID | ret time | peak area | pmol/µl | pmol/µl | pmol/mg liver | pmol/mg protein | ID | ret time | peak area | ||||||||
| 16 | wt | FED | 1 | 17.208 | 3400593 | 0.3334337853817084 | 3.3343378538170843 | 16.67168926908542 | 0.020454187036744995 | 163.01492930650832 | 18.132 | 36984695 | 0.4072080267405086 | 4.072080267405086 | 20.36040133702543 | 199.08296820058325 | 1 | wt | 18.346 | 65639957 | -0.5952219445570988 | -5.952219445570988 | -29.761097227854936 | -291.0024942510843 | 1 | wt | 21.49 | 3003131 | 0.14807029591112233 | |||
| 14 | wt | FED | 2 | 17.219 | 5669582 | 1.1365731946949695 | 11.365731946949696 | 56.82865973474848 | 0.020966724491405138 | 542.0842893990826 | 18.141 | 31055366 | 0.1963913585436795 | 1.963913585436795 | 9.819567927183975 | 93.66811617340895 | 2 | wt | 18.356 | 73031054 | -0.05646102878655391 | -0.5646102878655391 | -2.8230514393276955 | -26.92887427871669 | 2 | wt | 21.5 | 1579583 | 0.10097017098137409 | |||
| 13 | wt | FED | 3 | 17.215 | 1644225 | -0.2882564731681188 | -2.882564731681188 | -14.412823658405939 | 0.019902223624034064 | -144.8363150839177 | 18.142 | 28131910 | 0.09244818832323311 | 0.924481883232331 | 4.622409416161655 | 46.451185590936696 | 3 | wt | 18.358 | 73106983 | -0.05092631890877234 | -0.5092631890877234 | -2.5463159454386166 | -25.58825580035859 | 3 | wt | 21.502 | 2689212 | 0.1376838360813016 | |||
| 20 | M/M MCAD KO | FED | 4 | 17.212 | 3945851 | 0.5264352446410218 | 5.264352446410218 | 26.32176223205109 | 0.021676391736319193 | 242.86110485767327 | 18.138 | 45949032 | 0.7259340847706092 | 7.259340847706092 | 36.29670423853046 | 334.89618272320354 | 4 | M/M MCAD KO | 18.352 | 62183032 | -0.8472083653482331 | -8.472083653482331 | -42.36041826741165 | -390.84381554551805 | 4 | M/M MCAD KO | 21.496 | 1754700 | 0.10676416779589859 | |||
| 17 | wt | FED | 5 | 17.222 | 4912247 | 0.8685042068980879 | 8.685042068980879 | 43.42521034490439 | 0.02246491089733481 | 386.60478595582833 | 18.148 | 43525522 | 0.6397664419324909 | 6.397664419324909 | 31.988322096624543 | 284.78476716700067 | 5 | wt | 18.362 | 67126982 | -0.4868278927981116 | -4.868278927981116 | -24.34139463990558 | -216.70590861585296 | 5 | wt | 21.503 | 2477849 | 0.13069058843139703 | |||
| 18 | M/M MCAD KO | FED | 6 | 17.226 | 4980461 | 0.8926494767371638 | 8.926494767371638 | 44.63247383685819 | 0.021912947484623874 | 407.3616647708065 | 18.153 | 38217295 | 0.45103298993944924 | 4.510329899394493 | 22.551649496972463 | 205.8294486654227 | 6 | M/M MCAD KO | 18.367 | 72793254 | -0.07379503830777052 | -0.7379503830777052 | -3.689751915388526 | -33.6764546894259 | 6 | M/M MCAD KO | 21.511 | 1708525 | 0.10523640177140828 | |||
| 22 | M/M MCAD KO | FED | 7 | 17.219 | 5412745 | 1.04566225750233 | 10.4566225750233 | 52.283112875116494 | 0.02345055984860432 | 445.9007649510608 | 18.145 | 40420919 | 0.5293826092030404 | 5.293826092030404 | 26.46913046015202 | 225.74412407239268 | 7 | M/M MCAD KO | 18.361 | 87675485 | 1.0110188130418725 | 10.110188130418724 | 50.550940652093615 | 431.1277937793216 | 7 | M/M MCAD KO | 21.503 | 2483514 | 0.13087802307197607 | |||
| 21 | M/M MCAD KO | FED | 8 | 17.222 | 5118420 | 0.941481934615738 | 9.41481934615738 | 47.0740967307869 | 0.021282132155811383 | 442.38139662085183 | 18.148 | 36800615 | 0.4006630817253691 | 4.006630817253691 | 20.033154086268453 | 188.26266033498078 | 8 | M/M MCAD KO | 18.364 | 76416886 | 0.19034319377543052 | 1.9034319377543052 | 9.517159688771525 | 89.43802828677326 | 8 | M/M MCAD KO | 21.508 | 1387612 | 0.09461853543210655 | |||
| 19 | M/M MCAD KO | FED | 9 | 17.223 | 5448575 | 1.0583447716245642 | 10.583447716245642 | 52.917238581228204 | 0.021636965778268413 | 489.13733213348115 | 18.15 | 34757716 | 0.32802802291135585 | 3.2802802291135587 | 16.401401145567792 | 151.60537123038685 | 9 | M/M MCAD KO | 18.366 | 73214592 | -0.04308235158089766 | -0.4308235158089766 | -2.154117579044883 | -19.911457097264726 | 9 | M/M MCAD KO | 21.511 | 1965825 | 0.11374954087484858 | |||
| 15 | wt | FED | 10 | 17.226 | 5734026 | 1.1593840219373157 | 11.593840219373156 | 57.96920109686578 | 0.02293802239394417 | 505.44201327635056 | 18.151 | 35774994 | 0.36419723507514945 | 3.6419723507514945 | 18.209861753757473 | 158.77447010048283 | 10 | wt | 18.369 | 79707498 | 0.43020652320616914 | 4.3020652320616914 | 21.510326160308455 | 187.55170599177166 | 10 | wt | 21.512 | 1832365 | 0.1093338255824073 | |||
| KO17-13 | M/M MCAD KO | COLD | 11 | 17.224 | 17942363 | 5.4806903688950275 | 54.80690368895027 | 274.0345184447513 | 0.01556536823844819 | 3521.079800320504 | 18.154 | 25560348 | 0.001016572215984838 | 0.01016572215984838 | 0.0508286107992419 | 0.6530987255886382 | 11 | M/M MCAD KO | 18.362 | 108607720 | 2.5368369828919297 | 25.3683698289193 | 126.8418491445965 | 1629.7956746218574 | 11 | M/M MCAD KO | n.d. | |||||
| KO17-12* | M/M MCAD KO | COLD | 12 | 17.218 | 1697535 | -0.26938667613054396 | -2.6938667613054395 | -13.469333806527196 | 0.016945276770225514 | -158.9744917025388 | 18.151 | 39997684 | 0.5143345333475287 | 5.1433453334752866 | 25.71672666737643 | 303.5267823133252 | 12 | M/M MCAD KO | 18.365 | 73190484 | -0.04483966151166928 | -0.4483966151166928 | -2.2419830755834638 | -26.461451246672404 | 12 | M/M MCAD KO | 21.511 | 1683892 | 0.1044213836776071 | |||
| WT17-12 | wt | COLD | 13 | 17.226 | 14526789 | 4.271701658581433 | 42.717016585814335 | 213.58508292907166 | 0.02514587604478789 | 1698.7682795274297 | 18.157 | 75719875 | 1.7844332725658711 | 17.84433272565871 | 89.22166362829354 | 709.6325733044958 | 13 | wt | 18.368 | 108985672 | 2.5643871240465255 | 25.643871240465256 | 128.21935620232628 | 1019.804249205332 | 13 | wt | n.d. | |||||
| WT17-11 | wt | COLD | 14 | 17.219 | 1372440 | -0.38445845755861374 | -3.844584575586137 | -19.222922877930685 | 0.018719444882510643 | -205.3791979257936 | 18.148 | 32951056 | 0.26379241686478916 | 2.6379241686478916 | 13.189620843239457 | 140.9189313681237 | 14 | wt | 18.364 | 78524329 | 0.3439615160101711 | 3.4396151601017113 | 17.198075800508555 | 183.74557481217315 | 14 | wt | 21.548 | 1653292 | 0.103408938840587 | |||
| KO17-14 | M/M MCAD KO | COLD (outlier) | 15 | 17.226 | 43204314 | 14.422500313430039 | 144.22500313430038 | 721.1250156715018 | 0.018798296798612207 | 7672.237792572137 | 18.158 | 72252951 | 1.6611671548362554 | 16.611671548362555 | 83.05835774181277 | 883.679608122206 | 15 | M/M MCAD KO | 18.373 | 53169339 | -1.5042455406355484 | -15.042455406355483 | -75.2122770317774 | -800.2031017760077 | 15 | M/M MCAD KO | n.d. | |||||
| KO17-16 | M/M MCAD KO | COLD | 16 | 17.229 | 16612471 | 5.009957070791585 | 50.09957070791585 | 250.49785353957924 | 0.02021763128844031 | 2478.0138678540907 | 18.159 | 50727874 | 0.8958453088364472 | 8.958453088364472 | 44.79226544182236 | 443.1010221007731 | 16 | M/M MCAD KO | 18.371 | 107389115 | 2.4480089312830056 | 24.480089312830057 | 122.40044656415027 | 1210.8287545448939 | 16 | M/M MCAD KO | nd. | |||||
| KO17-15 | M/M MCAD KO | COLD | 17 | 17.219 | 13612837 | 3.9481959609246027 | 39.481959609246026 | 197.40979804623012 | 0.01891657467276455 | 2087.1621999352146 | 18.15 | 72540471 | 1.6713898981584683 | 16.713898981584684 | 83.56949490792341 | 883.5584280302498 | 17 | M/M MCAD KO | 18.364 | 98861245 | 1.8263849625037898 | 18.2638496250379 | 91.31924812518949 | 965.4945433294314 | 17 | M/M MCAD KO | nd. | |||||
| KO17-10 | M/M MCAD KO | COLD | 18 | 17.214 | 1561356 | -0.3175890788838718 | -3.175890788838718 | -15.879453944193589 | 0.016945276770225514 | -187.42041407190612 | 18.141 | 38293308 | 0.45373562414976976 | 4.537356241497697 | 22.686781207488487 | 267.7652482767511 | 18 | M/M MCAD KO | 18.355 | 81800101 | 0.5827431099434545 | 5.827431099434545 | 29.137155497172724 | 343.89707400199586 | 18 | M/M MCAD KO | 21.5 | 1922919 | 0.11232993439847 | |||
| WT17-16 | wt | COLD | 19 | 17.222 | 9353006 | 2.4403710428160004 | 24.403710428160004 | 122.01855214080001 | 0.022228355149030123 | 1097.863978892959 | 18.149 | 70021166 | 1.581816274183207 | 15.818162741832069 | 79.09081370916034 | 711.6209290241727 | 19 | wt | 18.365 | 100803188 | 1.9679394555721665 | 19.679394555721665 | 98.39697277860832 | 885.3284205592839 | 19 | wt | 21.512 | 5252075 | 0.2224798299486172 | |||
| KO17-9 | M/M MCAD KO | COLD | 20 | 17.228 | 16718825 | 5.047602510561501 | 50.47602510561501 | 252.38012552807504 | 0.021479261946065285 | 2349.988804660094 | 18.158 | 80879736 | 1.967891587052849 | 19.678915870528492 | 98.39457935264245 | 916.1821258077914 | 20 | M/M MCAD KO | 18.372 | 89394825 | 1.1363470549687378 | 11.363470549687378 | 56.81735274843689 | 529.04380877803 | 20 | M/M MCAD KO | nd. | |||||
| KO17-11 | M/M MCAD KO | COLD | 21 | 17.222 | 15164850 | 4.49755199749706 | 44.975519974970595 | 224.87759987485296 | 0.020375335120643427 | 2207.351177718952 | 18.154 | 64205398 | 1.3750372553826418 | 13.750372553826418 | 68.75186276913209 | 674.8538108654283 | 21 | M/M MCAD KO | 18.369 | 95474801 | 1.5795361292865195 | 15.795361292865195 | 78.97680646432597 | 775.2197055577262 | 21 | M/M MCAD KO | nd. | |||||
| WT17-13 | wt | COLD | 22 | 17.223 | 17679846 | 5.387768918718455 | 53.87768918718456 | 269.3884459359228 | 0.02293802239394417 | 2348.8375877341855 | 18.152 | 64076388 | 1.3704503184182613 | 13.704503184182613 | 68.52251592091307 | 597.4579215599997 | 22 | wt | 18.369 | 108389416 | 2.5209240997358338 | 25.209240997358336 | 126.04620498679168 | 1099.0154497370177 | 22 | wt | 21.511 | 12051036 | 0.44743319470518744 | |||
| WT17-15 | wt | COLD | 23 | 17.225 | 6451484 | 1.4133380051109115 | 14.133380051109114 | 70.66690025554557 | 0.021794669610471534 | 648.4787474969819 | 18.155 | 66966212 | 1.4731977064203832 | 14.731977064203832 | 73.65988532101916 | 675.944041708513 | 23 | wt | 18.371 | 77098623 | 0.24003720407385631 | 2.400372040738563 | 12.001860203692814 | 110.13573885906823 | 23 | wt | nd. | |||||
| WT17-9* | wt | COLD | 24 | 17.235 | 8876585 | 2.2717353726895992 | 22.717353726895993 | 113.58676863447997 | 0.020966724491405138 | 1083.49559971604 | 18.164 | 75406604 | 1.7732949551504116 | 17.732949551504117 | 88.66474775752057 | 845.7663264842995 | 24 | wt | 18.381 | 87712904 | 1.0137464047251121 | 10.137464047251122 | 50.68732023625561 | 483.5025161611085 | 24 | wt | nd | 0.04870740454363787 | ||||
| WT17-10 | wt | COLD | 25 | 17.231 | 11074100 | 3.0495755909793445 | 30.495755909793445 | 152.47877954896722 | 0.018601167008358302 | 1639.4539060958055 | 18.159 | 70294969 | 1.5915513110149118 | 15.915513110149117 | 79.57756555074558 | 855.6190642768594 | 25 | wt | 18.376 | 95063860 | 1.5495813134469136 | 15.495813134469136 | 77.47906567234568 | 833.0559651180059 | 25 | wt | nd | 0.04870740454363787 | ||||
| WT17-14 | wt | COLD | 26 | 17.228 | 12850930 | 3.678508651761077 | 36.78508651761077 | 183.92543258805384 | 0.021282132155811383 | 1728.4493042472764 | 18.156 | 74268078 | 1.7328147831148468 | 17.328147831148467 | 86.64073915574232 | 814.2110811212482 | 26 | wt | 18.373 | 93634566 | 1.4453954606100012 | 14.453954606100012 | 72.26977303050006 | 679.1591415878488 | 26 | wt | 21.619 | 2742916 | 0.13946070985670447 | |||
| 89 (38) | wt | FASTED | 27 | 17.233 | 7214413 | 1.6833870650261236 | 16.833870650261236 | 84.16935325130618 | 0.026486358618514425 | 635.567572451959 | 18.154 | 72066687 | 1.6545445579311058 | 16.545445579311057 | 82.72722789655528 | 624.6780018958704 | 27 | wt | 18.376 | 106966727 | 2.417219706667162 | 24.172197066671618 | 120.86098533335809 | 912.628172669037 | 27 | wt | nd | 0.04870740454363787 | ||||
| 92 (41) | M/M MCAD KO | FASTED | 28 | 17.232 | 5423269 | 1.0493873699394607 | 10.493873699394607 | 52.46936849697303 | 0.02845765652105346 | 368.75396579585043 | 18.154 | 94357994 | 2.4471096308917484 | 24.471096308917485 | 122.35548154458742 | 859.9125613457788 | 28 | M/M MCAD KO | 18.374 | 113685287 | 2.906957229833121 | 29.069572298331213 | 145.34786149165606 | 1021.5026763298323 | 28 | M/M MCAD KO | 21.528 | 1453627 | 0.09680273627903081 | |||
| 90 (39) | wt | FASTED | 29 | 17.231 | 8890220 | 2.276561665741697 | 22.76561665741697 | 113.82808328708485 | 0.026131524996057403 | 871.1935740777369 | 18.152 | 72397755 | 1.6663156458074218 | 16.66315645807422 | 83.31578229037109 | 637.664907064868 | 29 | wt | 18.374 | 103941222 | 2.1966808821234762 | 21.966808821234764 | 109.83404410617382 | 840.6248324408548 | 29 | wt | 21.535 | 1800751 | 0.10828783110274869 | |||
| 91 (40) | M/M MCAD KO | FASTED | 30 | 17.227 | 12848547 | 3.6776651566130645 | 36.776651566130646 | 183.8832578306532 | 0.02743258161173316 | 1340.6194169636933 | 18.152 | 78852821 | 1.8958248376756133 | 18.95824837675613 | 94.79124188378066 | 691.0850989192909 | 30 | M/M MCAD KO | 18.371 | 101342473 | 2.007249679813753 | 20.072496798137532 | 100.36248399068765 | 731.7028007875258 | 30 | M/M MCAD KO | 21.535 | 1634355 | 0.10278238106677491 | |||
| 88 (37) | M/M MCAD KO | FASTED | 31 | 17.23 | 7115248 | 1.6482862692423847 | 16.482862692423847 | 82.41431346211922 | 0.02660463649266677 | 619.5485022682847 | 18.154 | 88164472 | 2.226899608401217 | 22.26899608401217 | 111.34498042006084 | 837.0344052680567 | 31 | M/M MCAD KO | 18.374 | 103802116 | 2.1865409968061575 | 21.865409968061577 | 109.32704984030788 | 821.8646390484794 | 31 | M/M MCAD KO | 21.535 | 1115629 | 0.08561958823905952 | |||
| 84 (33) | wt | FASTED | 32 | 17.236 | 7705898 | 1.8573548421225872 | 18.573548421225873 | 92.86774210612936 | 0.02983756505283078 | 622.4887449206832 | 18.158 | 87847267 | 2.2156214180300338 | 22.15621418030034 | 110.78107090150169 | 742.5610682731736 | 32 | wt | 18.379 | 99977291 | 1.9077371575841755 | 19.077371575841756 | 95.38685787920878 | 639.3742767569375 | 32 | wt | 21.535 | 1159010 | 0.08705491077091472 | |||
| 83 (32) | wt | FASTED | 33 | 17.237 | 4475712 | 0.7139867270669542 | 7.1398672706695425 | 35.69933635334771 | 0.03031067654944015 | 235.5561829516939 | 18.154 | 70638644 | 1.603770639448626 | 16.03770639448626 | 80.18853197243129 | 529.1108025360945 | 33 | wt | 18.376 | 95320642 | 1.5682989822142688 | 15.682989822142687 | 78.41494911071344 | 517.40810854426 | 33 | wt | 21.528 | 1363871 | 0.09383303043616883 | |||
| 86 (35) | M/M MCAD KO | FASTED | 34 | 17.23 | 8968335 | 2.304211528724067 | 23.042115287240673 | 115.21057643620335 | 0.024593912632076963 | 936.9031935645597 | 18.156 | 86429378 | 2.1652085239482695 | 21.652085239482695 | 108.26042619741347 | 880.3839211513931 | 34 | M/M MCAD KO | 18.374 | 97153573 | 1.70190723875646 | 17.0190723875646 | 85.09536193782299 | 692.0034498848804 | 34 | M/M MCAD KO | 21.528 | 674077 | 0.0710102077594549 | |||
| 85 (34) | M/M MCAD KO | FASTED | 35 | 17.228 | 9189257 | 2.3824098648924243 | 23.82409864892424 | 119.1204932446212 | 0.02554013562529569 | 932.8101854450673 | 18.153 | 83986533 | 2.0783534272212565 | 20.783534272212563 | 103.91767136106282 | 813.7597457246058 | 35 | M/M MCAD KO | 18.372 | 95082560 | 1.5509444168028714 | 15.509444168028715 | 77.54722084014357 | 607.2577058936098 | 35 | M/M MCAD KO | 21.535 | 904830 | 0.07864500133713138 | |||
| 87 (36) | wt | FASTED | 36 | 17.238 | 4916879 | 0.8701437660779425 | 8.701437660779426 | 43.50718830389713 | 0.029127897807916733 | 298.7320855820376 | 18.158 | 86326261 | 2.161542209872182 | 21.615422098721822 | 108.07711049360911 | 742.0865810936388 | 36 | wt | 18.379 | 99604260 | 1.8805457240025567 | 18.805457240025568 | 94.02728620012783 | 645.6166992907532 | 36 | wt | nd | 0.04870740454363787 | ||||
| CoA | (Used Standards sheet, we used the standard curve suggested by Marianne) | pPanSH | (Used Standards2 sheet, as we considered the last point in the std curve) | dephosphoCoA | (Used Standards2 sheet, as we considered the last point in the std curve) | |||||||||||||||||||||||||||
| Fed | Fed | Fed | ||||||||||||||||||||||||||||||
| Animal ID | WT | MCAD | Animal ID | Animal ID | WT | MCAD | Animal ID | Animal ID | WT | MCAD | Animal ID | |||||||||||||||||||||
| 1 | 163.01492930650832 | 242.86110485767327 | 4 | 1 | 199.08296820058325 | 334.89618272320354 | 4 | |||||||||||||||||||||||||
| 2 | 542.0842893990826 | 407.3616647708065 | 6 | 2 | 93.66811617340895 | 205.8294486654227 | 6 | |||||||||||||||||||||||||
| 3 | 445.9007649510608 | 7 | 3 | 46.451185590936696 | 225.74412407239268 | 7 | ||||||||||||||||||||||||||
| 5 | 386.60478595582833 | 442.38139662085183 | 8 | 5 | 284.78476716700067 | 188.26266033498078 | 8 | |||||||||||||||||||||||||
| 10 | 505.44201327635056 | 489.13733213348115 | 9 | 10 | 158.77447010048283 | 151.60537123038685 | 9 | |||||||||||||||||||||||||
| Average | 399.28650448444245 | 405.5284526667747 | Average | 156.5523014464825 | 221.26755740527727 | Average | ||||||||||||||||||||||||||
| SD | 148.02472989782657 | 85.37275269406068 | SD | 82.86620225753512 | 61.82553316473118 | SD | ||||||||||||||||||||||||||
| Fasted | Fasted | Fasted | ||||||||||||||||||||||||||||||
| Animal ID | WT | MCAD | Animal ID | Animal ID | WT | MCAD | Animal ID | Animal ID | WT | MCAD | Animal ID | |||||||||||||||||||||
| 27 | 635.567572451959 | 368.75396579585043 | 28 | 27 | 624.6780018958704 | 859.9125613457788 | 28 | |||||||||||||||||||||||||
| 29 | 871.1935740777369 | 1340.6194169636933 | 30 | 29 | 637.664907064868 | 691.0850989192909 | 30 | |||||||||||||||||||||||||
| 32 | 622.4887449206832 | 619.5485022682847 | 31 | 32 | 742.5610682731736 | 837.0344052680567 | 31 | |||||||||||||||||||||||||
| 33 | 235.5561829516939 | 936.9031935645597 | 34 | 33 | 529.1108025360945 | 880.3839211513931 | 34 | |||||||||||||||||||||||||
| 36 | 298.7320855820376 | 932.8101854450673 | 35 | 36 | 742.0865810936388 | 813.7597457246058 | 35 | |||||||||||||||||||||||||
| Average | 532.7076319968221 | 839.7270528074911 | Average | 655.2202721727291 | 816.4351464818251 | Average | ||||||||||||||||||||||||||
| SD | 235.05620117937863 | 328.4189626653345 | SD | 80.39749963620784 | 66.51894924106212 | SD | ||||||||||||||||||||||||||
| Fasted + Cold | Fasted + Cold | Fasted + Cold | ||||||||||||||||||||||||||||||
| Animal ID | WT | MCAD | Animal ID | Animal ID | WT | MCAD | Animal ID | Animal ID | WT | MCAD | Animal ID | |||||||||||||||||||||
| 13 | 1698.7682795274297 | 3521.079800320504 | 11 | 13 | 709.6325733044958 | 0.6530987255886382 | 11 | |||||||||||||||||||||||||
| 14 | 12 | 14 | 140.9189313681237 | 303.5267823133252 | 12 | |||||||||||||||||||||||||||
| 19 | 1097.863978892959 | 15 | 19 | 711.6209290241727 | 883.679608122206 | 15 | ||||||||||||||||||||||||||
| 22 | 2348.8375877341855 | 2478.0138678540907 | 16 | 22 | 597.4579215599997 | 443.1010221007731 | 16 | |||||||||||||||||||||||||
| 23 | 648.4787474969819 | 2087.1621999352146 | 17 | 23 | 675.944041708513 | 883.5584280302498 | 17 | |||||||||||||||||||||||||
| 24 | 1083.49559971604 | 18 | 24 | 845.7663264842995 | 267.7652482767511 | 18 | ||||||||||||||||||||||||||
| 25 | 1639.4539060958055 | 2349.988804660094 | 20 | 25 | 855.6190642768594 | 916.1821258077914 | 20 | |||||||||||||||||||||||||
| 26 | 1728.4493042472764 | 2207.351177718952 | 21 | 26 | 814.2110811212482 | 674.8538108654283 | 21 | |||||||||||||||||||||||||
| Average | 1463.6210576729538 | 2528.7191700977714 | Average | 668.896358605964 | 546.6650155302642 | Average | ||||||||||||||||||||||||||
| SD | 518.6086195789794 | 513.3313035547104 | SD | 216.40899785845215 | 321.2878506294687 | SD |
