HepG2 qPCR statistical significance, palmitate/low-glucose (Fig. S12)

Selected Cell

Cell:

Value:

Rules Analysis

p values	points
0.1<p	0
0.1> p >0.05	1
0.01< p<0.05	2
0.005 < p <0.01	3
<0.005	4

Negative if down regulated
Positive if up regulated

Summary	Significance score versus WT * (using one-way Brown-Forsythe ANOVA)										p values										Points
Experiment number	KO4			KO9			KO21			Experiment number	Exp. 2			Exp. 3			Exp. 1			Experiment number	Exp. 2			Exp. 3			Exp. 1
GOI	Exp. 1	Exp. 2	Exp. 3	Exp. 1	Exp. 2	Exp. 3	Exp. 1	Exp. 2	Exp. 3	GOI	KO4	KO9	KO21	KO4	KO9	KO21	KO4	KO9	KO21	GOI	KO4	KO9	KO21	KO4	KO9	KO21	KO4	KO9	KO21
Acot1	-2	-1		0	0	0	-1	0	-4	Acot1	0.0606	0.986	0.1124		0.9979	0.0038	0.0441	0.9839	0.0568	Acot1	-1	0	0		0	-4	-2	0	-1
Acot2	-2	-4		0	0	0	-1	-2	-2	Acot2	0.0014	0.3324	0.0104		0.9824	0.01	0.0312	>0,9999	0.0691	Acot2	-4	0	-2		0	-2	-2	0	-1
Acot4	0	-2		0	0	0	0	0	0	Acot4	0.0469	0.1701	0.8529		0.2899	0.1302	0.7293	0.9927	>0,9999	Acot4	-2	0	0		0	0	0	0	0
Acot6	0	-1		0	-1	-1	0	0	-2	Acot6	0.0771	0.0748	0.8152		0.0787	0.0318	0.7762	0.9999	0.9875	Acot6	-1	-1	0		-1	-2	0	0	0
Acot7	0	0		0	0	0	0	0	0	Acot7	0.953	0.3047	0.7219		0.1666	0.9982	0.1108	0.9942	0.1495	Acot7	0	0	0		0	0	0	0	0
Acot8	-2	-2		4	2	2	-1	0	0	Acot8	0.0415	0.0117	0.3436		0.0155	0.9195	0.0237	0.0031	0.0672	Acot8	-2	2	0		2	0	-2	4	-1
Acot9	0	0		-3	-3	0	0	0	4	Acot9	0.7709	0.0086	0.9168		0.3203	3.0E-4	0.1442	0.0059	0.1122	Acot9	0	-3	0		0	4	0	-3	0
Acot11	0	0		0	0	0	-1	0	0	Acot11	0.3754	0.9576	0.2483		0.1628	0.3929	0.9877	0.4321	0.081	Acot11	0	0	0		0	0	0	0	-1
Acot12	0	0		0	0	0	0	2	0	Acot12	0.8861	0.9473	0.0262		0.3307	0.1862	0.986	0.983	0.7476	Acot12	0	0	2		0	0	0	0	0
Acot13	-2	0		4	4	4	0	0	2	Acot13	0.5358	0.0039	0.2633		0.0014	0.0297	0.0173	2.0E-4	0.5534	Acot13	0	4	0		4	2	-2	4	0
Nudt7	-4	-1		0	0	0	0	0	-1	Nudt7	0.0535	0.9944	0.9529		0.6439	0.0977	9.0E-4	0.3099	0.4043	Nudt7	-1	0	0		0	-1	-4	0	0
Nudt8	0	-4		0	0	0	0	0	-4	Nudt8	0.0039	0.5201	0.4563		0.2623	0.003	0.1979	0.9843	0.3782	Nudt8	-4	0	0		0	-4	0	0	0
Nudt19	-4	-4		-4	-4	-2	2	2	4	Nudt19	3.0E-4	6.0E-4	0.0159		0.0332	0.0023	5.0E-4	3.0E-4	0.0364	Nudt19	-4	-4	2		-2	4	-4	-4	2
SLC25A42	-4	-4		-2	-1	-4	-2	-4	-4	SLC25A42	2.0E-4	0.0504	7.0E-4		1.0E-4	<0,0001	0.0015	0.0431	0.0406	SLC25A42	-4	-1	-4		-4	-4	-4	-2	-2
SLC25A16	0	0		0	0	0	0	0	-2	SLC25A16	0.512	0.7328	0.9998		0.349	0.0182	0.9313	0.9959	0.365	SLC25A16	0	0	0		0	-2	0	0	0
SLC25A17	0	0		0	0	2	0	0	-2	SLC25A17	0.9941	0.9997	0.4342		0.022	0.0123	0.1237	0.9866	0.1938	SLC25A17	0	0	0		2	-2	0	0	0
pexCRAT	0	0		-4	-3	-4	-4	-4	-3	pexCRAT	0.999	0.0089	0.0041		0.0036	0.009	0.166	0.0018	0.0037	pexCRAT	0	-3	-4		-4	-3	0	-4	-4
pexCROT	2	0		0	-1	0	0	0	-1	pexCROT	0.3207	0.0814	0.678		0.8463	0.086	0.0259	0.6671	0.9655	pexCROT	0	-1	0		0	-1	2	0	0
CPT1a	-4	-4		-4	-2	0	-2	0	0	CPT1a	0.0034	0.0402	0.157		0.2732	0.3386	0.0028	0.0039	0.0121	CPT1a	-4	-2	0		0	0	-4	-4	-2
CPT2	-2	-4		0	-2	0	0	-2	0	CPT2	0.0012	0.0487	0.0194		0.3679	0.1409	0.0201	0.77	0.9927	CPT2	-4	-2	-2		0	0	-2	0	0
Pank1	0	0		2	0	0	1	0	0	Pank1	0.9746	0.6575	0.7864		0.4206	0.1881	0.1611	0.0166	0.0717	Pank1	0	0	0		0	0	0	2	1
Pank2	0	0		0	0	-1	-2	-4	-4	Pank2	0.6723	0.1841	0.0039		0.0816	4.0E-4	0.6195	0.1563	0.0141	Pank2	0	0	-4		-1	-4	0	0	-2
Pank3	0	0		0	0	0	-2	-2	-4	Pank3	0.1239	0.8743	0.032		0.1271	<0,0001	0.8664	0.1946	0.0379	Pank3	0	0	-2		0	-4	0	0	-2
Pank4	0	-2		0	0	4	0	0	0	Pank4	0.0269	0.8689	0.5249		0.0034	0.611	0.1469	0.9589	0.6393	Pank4	-2	0	0		4	0	0	0	0
PPCS	0	0		0	0	3	0	0	0	PPCS	0.5654	0.8139	0.9856		0.0088	0.9097	0.2978	0.8363	0.6178	PPCS	0	0	0		3	0	0	0	0
PPCDC	0	0		0	0	0	0	0	0	PPCDC	0.2858	0.585	0.9258		0.2863	0.3819	>0,9999	0.2642	0.9987	PPCDC	0	0	0		0	0	0	0	0
Coasy	0	0		-1	0	0	0	0	-2	Coasy	0.4614	0.4539	0.9849		0.8907	0.0362	0.6591	0.0923	0.9927	Coasy	0	0	0		0	-2	0	-1	0
ABCD3	0	2		0	2	1	0	0	-4	ABCD3	0.0276	0.0286	0.5145		0.0623	0.0032	0.2854	0.3875	0.9308	ABCD3	2	2	0		1	-4	0	0	0

																				0.1<p	0
																				0.1> p >0.05	1
																				0.01< p<0.05	2
																				0.005 < p <0.01	3
																				p<0.005	4

																				Negative if down regulated
																				Positive if up regulated