Selected Cell
Cell:
Value:
Note on data sheet
N1
N2
Combined_mean_clone_vs__mean_WT
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| The sheets: "N1", and "N2" contain the normalised data (peak area/mg protein) and all of the data processing steps to get to relative level. | |||||||||
| The data used for plotting, were: | |||||||||
| Fig. S5: The mean of all of the data points pertaining to a single clone (e.g. all of the data points from KO4 in experiment N1) to the mean of the WT data in the same experiment. | |||||||||
| The final data plotted in the figures are summarised in "Combined_mean_clone_vs__mean_WT" | |||||||||
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| area ratio / mg protein | ||||||||||||||||||||||||||||||
| Acyl-CoA chain length | C0 | C2:0 | C4:0 | C6:0 | C8:0 | C10:0 | C12:0 | C14:0 | C16:0 | C18:0 | ||||||||||||||||||||
| WT_1 | 0.1517513307984791 | 0.014440304182509505 | 0.004279087452471483 | 5.026615969581749E-4 | 6.882129277566541E-4 | 9.007604562737643E-4 | 0.002279467680608365 | 0.010965019011406844 | 0.06621406844106464 | 0.009033840304182509 | ||||||||||||||||||||
| WT_2 | 0.21761520270270268 | 0.013510472972972972 | 0.003888175675675676 | 6.040540540540541E-4 | 7.712837837837837E-4 | 7.95945945945946E-4 | 0.002111148648648649 | 0.010272297297297298 | 0.06949864864864866 | 0.007593581081081081 | ||||||||||||||||||||
| WT_3 | 0.26063973333333335 | 0.011678133333333333 | 0.005502133333333333 | 4.6426666666666666E-4 | 5.637333333333333E-4 | 6.922666666666666E-4 | 0.0016381333333333333 | 0.008998666666666665 | 0.0640024 | 0.006995466666666667 | ||||||||||||||||||||
| WT_4 | 0.05109629629629629 | 0.01145679012345679 | 0.0027413580246913576 | 4.6172839506172835E-4 | 6.836419753086419E-4 | 7.577160493827161E-4 | 0.001716358024691358 | 0.009658641975308642 | 0.07165185185185184 | 0.007696604938271605 | ||||||||||||||||||||
| WT_5 | 0.06661363636363636 | 0.008221753246753246 | 0.0016405844155844157 | 2.8733766233766234E-4 | 4.5616883116883115E-4 | 5.840909090909091E-4 | 0.0013555194805194805 | 0.006579870129870129 | 0.05611136363636364 | 0.006688961038961039 | ||||||||||||||||||||
| KO4_1 | 0.02538333333333333 | 0.012655238095238094 | 0.0012126190476190477 | 0.0010783333333333333 | 0.005984047619047619 | 9.016666666666666E-4 | 0.0010414285714285714 | 0.005865714285714286 | 0.04398714285714286 | 0.008317142857142856 | ||||||||||||||||||||
| KO4_2 | 0.07297785388127855 | 0.013755707762557077 | 0.0029682648401826487 | 0.001352054794520548 | 0.007561643835616438 | 0.0010077625570776257 | 0.0010831050228310502 | 0.0068940639269406395 | 0.05403013698630137 | 0.007902511415525114 | ||||||||||||||||||||
| KO4_3 | 0.0432103825136612 | 0.014312021857923496 | 0.001630054644808743 | 0.0015860655737704918 | 0.006233060109289618 | 9.497267759562841E-4 | 0.0010961748633879782 | 0.007363114754098361 | 0.050196994535519124 | 0.00797568306010929 | ||||||||||||||||||||
| KO4_4 | 0.021404677060133628 | 0.013497772828507794 | 0.0014612472160356346 | 0.0014910913140311803 | 0.00615902004454343 | 8.220489977728285E-4 | 0.001252783964365256 | 0.00753184855233853 | 0.056146547884187076 | 0.008873273942093541 | ||||||||||||||||||||
| KO4_5 | 0.08345994152046783 | 0.013390350877192984 | 0.0015634502923976609 | 0.001202923976608187 | 0.0060783625730994155 | 8.973684210526316E-4 | 0.0010321637426900585 | 0.007009941520467836 | 0.05906023391812865 | 0.008912573099415205 | ||||||||||||||||||||
| KO9_1 | 0.06359046511627907 | 0.009619302325581395 | 0.0028774418604651164 | 0.0011388372093023257 | 0.013934418604651163 | 0.0014000000000000002 | 0.001257674418604651 | 0.006890697674418605 | 0.0487746511627907 | 0.009145116279069767 | ||||||||||||||||||||
| KO9_2 | 0.0923774647887324 | 0.010683943661971833 | 0.0013194366197183099 | 7.374647887323945E-4 | 0.009430422535211268 | 0.0012904225352112677 | 0.001144225352112676 | 0.006036056338028169 | 0.04894760563380282 | 0.009561408450704226 | ||||||||||||||||||||
| KO9_3 | 0.1905065573770492 | 0.010414207650273222 | 0.0022972677595628414 | 0.0011581967213114752 | 0.012353825136612021 | 0.0015131147540983607 | 9.691256830601092E-4 | 0.006094808743169399 | 0.0503103825136612 | 0.008033879781420764 | ||||||||||||||||||||
| KO9_4 | 0.11098472906403942 | 0.01035024630541872 | 0.0030118226600985222 | 4.995073891625616E-4 | 0.006692118226600986 | 7.093596059113301E-4 | 6.625615763546799E-4 | 0.004680541871921183 | 0.04321379310344828 | 0.007697536945812809 | ||||||||||||||||||||
| KO9_5 | 0.13038491124260357 | 0.010187869822485207 | 0.0019976331360946746 | 0.0010494082840236687 | 0.013607692307692308 | 0.0013446745562130178 | 0.00136301775147929 | 0.006681065088757397 | 0.058536390532544384 | 0.009868639053254438 | ||||||||||||||||||||
| KO21_1 | 0.03459574036511156 | 0.006826166328600406 | 0.0023920892494929008 | 0.0025125760649087224 | 0.022352738336713997 | 0.0016403651115618663 | 0.0013789046653144017 | 0.005857606490872211 | 0.03882758620689655 | 0.006023123732251522 | ||||||||||||||||||||
| KO21_2 | 0.05317679324894515 | 0.006704008438818565 | 0.0030531645569620252 | 0.0032782700421940926 | 0.021229535864978902 | 0.0015784810126582278 | 0.0011824894514767931 | 0.006245358649789029 | 0.04295611814345991 | 0.006795780590717298 | ||||||||||||||||||||
| KO21_3 | 0.038812664907651714 | 0.008596833773087072 | 0.002003166226912929 | 0.0013664907651715038 | 0.0179221635883905 | 0.0017733509234828498 | 0.002463060686015831 | 0.007070184696569921 | 0.06005013192612137 | 0.007488654353562005 | ||||||||||||||||||||
| KO21_4 | 0.10050534759358289 | 0.007910962566844919 | 0.0036240641711229944 | 0.0033053475935828874 | 0.019441176470588233 | 0.001602139037433155 | 0.0016122994652406416 | 0.007455347593582888 | 0.05135561497326203 | 0.007588502673796791 | ||||||||||||||||||||
| KO21_5 | 0.04129735576923077 | 0.006889903846153846 | 0.003893990384615385 | 0.0020800480769230766 | 0.015686298076923073 | 0.001379326923076923 | 0.001541826923076923 | 0.006170192307692307 | 0.049105528846153844 | 0.0073384615384615385 | ||||||||||||||||||||
| AVERAGE | ||||||||||||||||||||||||||||||
| Acyl-CoA chain length | C0 | C2:0 | C4:0 | C6:0 | C8:0 | C10:0 | C12:0 | C14:0 | C16:0 | C18:0 | ||||||||||||||||||||
| WT | 0.14954323989888957 | 0.011861490771805169 | 0.0036102677803512536 | 4.640096750156573E-4 | 6.326081702702489E-4 | 7.461560054720004E-4 | 0.0018201254335602373 | 0.009294899016109916 | 0.06549566651558576 | 0.007601690805832578 | ||||||||||||||||||||
| KO4 | 0.04928723766177491 | 0.01352221828428389 | 0.001767127208208747 | 0.001342093798452748 | 0.0064032268363193034 | 9.157146837052073E-4 | 0.001101131232940583 | 0.00693293660791193 | 0.052684211236255815 | 0.008396236874857202 | ||||||||||||||||||||
| KO9 | 0.11756882551774073 | 0.010251113953146075 | 0.002300720407187893 | 9.166828785064852E-4 | 0.011203695362153548 | 0.0012515142902867955 | 0.0010793209563222813 | 0.006076633943258951 | 0.04995656458924948 | 0.008861316102052399 | ||||||||||||||||||||
| KO21 | 0.05367758037690441 | 0.007385574990700962 | 0.0029932949178212466 | 0.0025085465085560566 | 0.019326382467518943 | 0.0015947326016426042 | 0.0016357162382249178 | 0.006559737947701272 | 0.04845899601917874 | 0.007046904577757832 | ||||||||||||||||||||
| Replicates vs. mean WT | ||||||||||||||||||||||||||||||
| Acyl-CoA chain length | C0 | C2:0 | C4:0 | C6:0 | C8:0 | C10:0 | C12:0 | C14:0 | C16:0 | C18:0 | ||||||||||||||||||||
| WT_1 | 1.0147655681465941 | 1.2174105650222475 | 1.1852548655144794 | 1.083299818998846 | 1.0878976277885424 | 1.2072012416545046 | 1.2523684569088385 | 1.1796813491359375 | 1.0109686940174574 | 1.18839880954525 | ||||||||||||||||||||
| WT_2 | 1.4551991975688001 | 1.1390198106537708 | 1.0769770865299593 | 1.3018134891986277 | 1.2192124920775098 | 1.0667285930942143 | 1.1598918457609586 | 1.105154265742248 | 1.0611182746282966 | 0.9989331682965487 | ||||||||||||||||||||
| WT_3 | 1.7429054867980611 | 0.9845417880433986 | 1.5240236093506656 | 1.000553849768328 | 0.8911255968959548 | 0.9277773838042827 | 0.9000112317144427 | 0.9681295785000116 | 0.9772005295154846 | 0.9202514079235147 | ||||||||||||||||||||
| WT_4 | 0.34168242129061765 | 0.9658811311213633 | 0.7593226296429022 | 0.9950835508896405 | 1.080672061216964 | 1.0154927975194719 | 0.9429888693627525 | 1.0391336106576614 | 1.0939937810206286 | 1.012485923837655 | ||||||||||||||||||||
| WT_5 | 0.4454473261959266 | 0.6931467051592201 | 0.45442180896199297 | 0.6192492911445576 | 0.721092222021029 | 0.7827999839275263 | 0.744739596253007 | 0.7079011959641412 | 0.8567187208181327 | 0.879930690397033 | ||||||||||||||||||||
| KO4_1 | 0.1697390891791279 | 1.0669180070788122 | 0.3358806386104325 | 2.3239457955202045 | 9.459327116327389 | 1.2084157469138024 | 0.5721740668122504 | 0.6310681025740927 | 0.6716038662905338 | 1.0941174890672125 | ||||||||||||||||||||
| KO4_2 | 0.48800503406654117 | 1.1596946814859455 | 0.8221730411071773 | 2.9138504374395318 | 11.953123894030835 | 1.3506057040178068 | 0.595071637844461 | 0.7417040158254382 | 0.8249421658064052 | 1.0395728552208048 | ||||||||||||||||||||
| KO4_3 | 0.2889490861832134 | 1.2065955395710675 | 0.45150519129917566 | 3.418173497604274 | 9.85295543468379 | 1.2728260162638638 | 0.6022523740266718 | 0.7921672673728475 | 0.7664170349892375 | 1.0491985617186321 | ||||||||||||||||||||
| KO4_4 | 0.1431336988191906 | 1.1379491067507363 | 0.40474759905301694 | 3.213491860877396 | 9.735916059876859 | 1.1017119633752996 | 0.6882954005619057 | 0.8103206435362377 | 0.8572559204482039 | 1.1672763558451116 | ||||||||||||||||||||
| KO4_5 | 0.5580990593549897 | 1.1288927450014903 | 0.43305660065069973 | 2.5924545141598525 | 9.608416170949472 | 1.202655228225333 | 0.5670838523865388 | 0.7541708100667052 | 0.9017426199346228 | 1.1724461474514094 | ||||||||||||||||||||
| KO9_1 | 0.4252312920281411 | 0.810969085643647 | 0.7970161870334066 | 2.454339361057283 | 22.026934300735334 | 1.8762832299585845 | 0.6909822781524404 | 0.7413418545457728 | 0.7447004322214811 | 1.2030371285363197 | ||||||||||||||||||||
| KO9_2 | 0.6177307971339355 | 0.9007252012004787 | 0.3654677990644611 | 1.589330629167398 | 14.907209515145231 | 1.7294272588411015 | 0.6286519220131582 | 0.6493945041862723 | 0.7473411332054303 | 1.2578002308865297 | ||||||||||||||||||||
| KO9_3 | 1.2739228968548233 | 0.877984719680249 | 0.6363150600810374 | 2.49606157732033 | 19.528399595810615 | 2.027879884369758 | 0.5324499428396322 | 0.6557154340898033 | 0.7681482636975535 | 1.0568543218380546 | ||||||||||||||||||||
| KO9_4 | 0.7421581151985162 | 0.8725923667218386 | 0.834238024251346 | 1.0765020991118484 | 10.578614916943181 | 0.9506853804153278 | 0.3640197341007885 | 0.5035602714788906 | 0.6597962186271492 | 1.0126085291323201 | ||||||||||||||||||||
| KO9_5 | 0.8718876983724607 | 0.858902984328229 | 0.5533199357030295 | 2.2616086269930857 | 21.510459313035952 | 1.8021359425532049 | 0.7488592414277588 | 0.7187883458634438 | 0.8937444818370494 | 1.298216318622495 | ||||||||||||||||||||
| KO21_1 | 0.23134272327189595 | 0.5754897474461004 | 0.6625794525579949 | 5.4149217143455886 | 35.33425489456602 | 2.198421107023337 | 0.7575877134012735 | 0.6301958182353363 | 0.592826797138661 | 0.7923400051512404 | ||||||||||||||||||||
| KO21_2 | 0.35559476499840104 | 0.5651910512592592 | 0.845689223823994 | 7.06508984340353 | 33.5587443581541 | 2.1154838948990546 | 0.6496747035525992 | 0.6719124800564886 | 0.6558619894834997 | 0.8939827683471516 | ||||||||||||||||||||
| KO21_3 | 0.25954142048743933 | 0.7247684071484332 | 0.5548525341569082 | 2.9449617944397253 | 28.3305914002568 | 2.376648998973171 | 1.3532367828067688 | 0.760652125893555 | 0.9168565665612618 | 0.9851300907708792 | ||||||||||||||||||||
| KO21_4 | 0.6720821861391889 | 0.6669450509247389 | 1.0038214314314375 | 7.123445418398557 | 30.731782142938503 | 2.147190434284155 | 0.8858177769028369 | 0.8020902196636329 | 0.7841070670078778 | 0.9982651054386916 | ||||||||||||||||||||
| KO21_5 | 0.2761566206346277 | 0.5808632303227168 | 1.0785876897576077 | 4.482768763071348 | 24.79623061178916 | 1.8485771245711462 | 0.8470992683515491 | 0.6638256421073679 | 0.749752334140586 | 0.9653722738671414 | ||||||||||||||||||||
| Mean Clone vs. mean WT (for WT, this should be 1 everywhere) | ||||||||||||||||||||||||||||||
| Acyl-CoA chain length | C0 | C2:0 | C4:0 | C6:0 | C8:0 | C10:0 | C12:0 | C14:0 | C16:0 | C18:0 | ||||||||||||||||||||
| WT | 1 | 1.0000000000000002 | 1 | 1 | 0.9999999999999998 | 0.9999999999999998 | 0.9999999999999998 | 1 | 1 | 1.0000000000000002 | ||||||||||||||||||||
| KO4 | 0.32958519352061255 | 1.1400100159776103 | 0.4894726141441003 | 2.8923832211202516 | 10.121947735173668 | 1.227242931759221 | 0.6049754663263657 | 0.7458861678750642 | 0.8043923214938007 | 1.1045222818606342 | ||||||||||||||||||||
| KO9 | 0.7861861599175755 | 0.8642348715148886 | 0.6372714012266562 | 1.9755684587299889 | 17.710323528334065 | 1.6772823392275953 | 0.5929926237067557 | 0.6537600820328365 | 0.7627461059177327 | 1.1657033058031439 | ||||||||||||||||||||
| KO21 | 0.3589435431063106 | 0.6226514974202496 | 0.8291060663455884 | 5.406237506731751 | 30.550320681540917 | 2.137264311950173 | 0.8986832490030056 | 0.7057352571912762 | 0.7398809508663773 | 0.9270180487150208 |
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| area ratio / mg protein | ||||||||||||||||
| Acyl-CoA chain length | C0 | C2:0 | C4:0 | C6:0 | C8:0 | C8:1 | C10:0 | C10:1 | C12:0 | C12:1 | C14:0 | C14:1 | C16:0 | C16:1 | C18:0 | C18:1 |
| WT_1 | 0.013705235594572715 | 0.011353251744556097 | 0.002110558959898197 | 6.874721352285566E-4 | 3.597397374422252E-4 | 1.1624521632664077E-4 | 4.374136514955599E-4 | 2.3255933569795326E-4 | 0.0029011504233967876 | 0.001996783872239013 | 0.010946714529266363 | 0.007391836983281591 | 0.04929814012197632 | 0.04149784305608678 | 0.006328973240536884 | 0.03298754921709026 |
| WT_2 | 0.012997561839443604 | 0.01025144348039542 | 0.002195480138334611 | 8.755835933444905E-4 | 5.165880698142543E-4 | 1.3631470931365318E-4 | 8.014144686292652E-4 | 3.1108633685655455E-4 | 0.0035611499016698737 | 0.00239664668942926 | 0.012298706945610043 | 0.0073446865652769975 | 0.048418275606363075 | 0.039516215572643565 | 0.006062707401540652 | 0.03118410295068125 |
| WT_3 | 0.012692428144100472 | 0.009680182966188253 | 0.0019818650997501797 | 5.336955351298216E-4 | 3.1073085242235384E-4 | 5.2728341130922244E-5 | 4.2805969473739294E-4 | 1.8728517753312395E-4 | 0.0031620221573008173 | 0.0021263804244501536 | 0.010714421303173608 | 0.007037475949035552 | 0.04820011628468723 | 0.04768554514221342 | 0.005076193717832968 | 0.031074845433982815 |
| WT_4 | 0.011423309736917896 | 0.010287075788406857 | 0.0022161690742077793 | 6.156303162709566E-4 | 4.091971931357499E-4 | 7.428669365011345E-5 | 5.148811512463631E-4 | 2.1230403315606434E-4 | 0.002593722091583153 | 0.0016863824648437717 | 0.009625004195080137 | 0.006706121627017496 | 0.048833849234191894 | 0.04231419853814611 | 0.005566126656478677 | 0.029837966996517484 |
| WT_5 | 0.009572667146569565 | 0.009838126243748243 | 0.0018268022638525461 | 5.114879743846024E-4 | 3.745188403250757E-4 | 9.205663805401594E-5 | 5.787740655829204E-4 | 2.8057990673894227E-4 | 0.00297674964350432 | 0.0013536204075790877 | 0.010738945948275427 | 0.008528462876336794 | 0.04145057514302594 | 0.046497990315141575 | 0.005080249420265529 | 0.031660339028799764 |
| KO4_1 | 0.005404594948596347 | 0.009619568693247083 | 9.191402229708326E-4 | 9.62281376645043E-4 | 0.016958380129075107 | 5.677091985315833E-4 | 0.0014951149922718056 | 4.613103521069245E-4 | 0.0031327732560334754 | 0.0033343008697165975 | 0.006427939531201492 | 0.007356895142451149 | 0.030859950415327714 | 0.048511039115795594 | 0.005321689214570749 | 0.035787754811224017 |
| KO4_2 | 0.006940469382577004 | 0.010648075171102346 | 0.0016064604201354808 | 9.815219270305708E-4 | 0.0129400124490259 | 5.127990011779282E-4 | 0.0012048719560188144 | 4.0700103228653243E-4 | 0.0021747232196773943 | 0.00231273229049061 | 0.007448175600345592 | 0.006095865534077859 | 0.03476921573791204 | 0.047845509657009073 | 0.005568982809357293 | 0.03533286098021801 |
| KO4_3 | 0.008610211718167489 | 0.01104387400876479 | 0.0014818686520857564 | 0.0014534718595461697 | 0.017516248922488015 | 7.821488684854361E-4 | 0.0012955074032213645 | 5.102361075930616E-4 | 0.0027887970173051304 | 0.0028656243330244338 | 0.007081935455182102 | 0.00690296526929087 | 0.036966714564348746 | 0.049156675205010686 | 0.0063543537148649295 | 0.03959975634012874 |
| KO4_4 | 0.00532785876347442 | 0.01104079073863198 | 0.001219056689621805 | 0.001340357388562858 | 0.012868918336888371 | 5.033924398104647E-4 | 0.0010516882923043698 | 4.174040447644816E-4 | 0.0025880179875274706 | 0.002038874133284781 | 0.008104021754749606 | 0.007182896116186967 | 0.03485880461694736 | 0.05026360157741392 | 0.005065680102208236 | 0.0377531911229484 |
| KO4_5 | 0.006456416335477007 | 0.009922721929339885 | 9.358041005034678E-4 | 0.0011774653983821937 | 0.012807357559630842 | 7.199473079204784E-4 | 0.0012398162328243441 | 4.3427379223050694E-4 | 0.0024308777160717628 | 0.001868485647013549 | 0.0072499304801284265 | 0.006418234134664911 | 0.0302410042929387 | 0.04355850794198126 | 0.004693354427015324 | 0.03282466391673375 |
| KO9_1 | 0.005072660488049861 | 0.010670284529320517 | 0.001703699972326744 | 0.0018408488877877242 | 0.01567066079497177 | 6.221172541024062E-4 | 0.0011569787703265447 | 6.932275686124228E-4 | 0.0012534369956984555 | 0.004546125655003502 | 0.005728875311222967 | 0.007044599057018731 | 0.045922099149566215 | 0.06423474017211177 | 0.0087478265949094 | 0.05282682658026746 |
| KO9_2 | 0.006108038871782257 | 0.009874707282995493 | 0.002108373159635015 | 0.0019197985239848198 | 0.01625295481184999 | 7.677915904664183E-4 | 8.272957346281442E-4 | 4.6164184065266585E-4 | 0.0013901872638200387 | 0.0030121975848553004 | 0.0049851922179470634 | 0.004855993501700802 | 0.03526291682635112 | 0.052936236709272465 | 0.005176821213450295 | 0.035604812040207816 |
| KO9_3 | 0.006432546238198712 | 0.009601613572461778 | 0.0019899131812660454 | 0.0019423817043044418 | 0.012913749979084206 | 8.537408867791929E-4 | 6.7237306243366E-4 | 5.609280423806177E-4 | 9.943636246716888E-4 | 0.0026099249471752146 | 0.005114888203291456 | 0.005395976320228065 | 0.037275314060363364 | 0.05385734173974029 | 0.006624368878676986 | 0.038536444902052464 |
| KO9_4 | 0.006239736455245463 | 0.01167477260981714 | 0.002189287451394693 | 0.0024053909256860923 | 0.014248256858616205 | 9.060389963058198E-4 | 0.0010610595800712688 | 4.294441952474608E-4 | 0.001463209840987918 | 0.003403070981232718 | 0.005034812744916243 | 0.0063878876859460275 | 0.042896380259774394 | 0.06729261157375904 | 0.00782552322230684 | 0.04633831167560263 |
| KO9_5 | 0.006003541290736709 | 0.009330375487627891 | 0.0019007935318028691 | 0.001732004127976903 | 0.013027390303143533 | 9.915545179882688E-4 | 8.516225224080801E-4 | 4.3176958147817E-4 | 8.515526455899899E-4 | 0.0029445826777707565 | 0.0037003042670290054 | 0.005432593614364873 | 0.03288987480814997 | 0.050396609904936704 | 0.006235942077360642 | 0.034762483088103945 |
| KO21_1 | 0.006385120596051441 | 0.011799088728954073 | 0.0011122509400368174 | 9.17057777425343E-4 | 0.006408078718864795 | 2.1145827316908732E-4 | 6.233114818758339E-4 | 3.095261070950856E-4 | 0.0010501171996511315 | 0.0010359461264773367 | 0.0047175067514547515 | 0.0032465741919655696 | 0.04041941608568562 | 0.034490251477455026 | 0.005543183467613903 | 0.02676316323455895 |
| KO21_2 | 0.008142062898483559 | 0.01183784141511915 | 0.001070241903217798 | 9.96901389970013E-4 | 0.006961146273866992 | 3.6727919147521717E-4 | 6.340203598601275E-4 | 3.057008083865954E-4 | 0.0012485125373862746 | 0.0010197580863991822 | 0.0049861037970767704 | 0.0052226628024586216 | 0.045224541250030025 | 0.03953975379635075 | 0.005785424878543587 | 0.02898419319657001 |
| KO21_3 | 0.010516154174469057 | 0.008189772881205683 | 8.25536660387641E-4 | 6.209254319546988E-4 | 0.009420712670235228 | 3.274151400248951E-4 | 9.506831166554824E-4 | 2.0065421951574654E-4 | 0.0012115248524601756 | 0.0010770363765790214 | 0.0026663722969171516 | 0.002122606621125924 | 0.02546069887513195 | 0.027503937761336864 | 0.005560503406120265 | 0.02465354786323438 |
| KO21_4 | 0.006205596081309513 | 0.011518772457488275 | 9.221174824965883E-4 | 6.008865018727919E-4 | 0.004040872838643893 | 2.3371691479483352E-4 | 3.631862599820736E-4 | 2.7545988617930803E-4 | 6.244689693992853E-4 | 9.941462036676453E-4 | 0.005035281526185453 | 0.003962916832607063 | 0.036839116510919916 | 0.032522740476426976 | 0.005141987472548259 | 0.02624906833171176 |
| KO21_5 | 0.005486352009416683 | 0.013053984779744425 | 0.0013472581943547732 | 0.0010170462576412713 | 0.005543885928132579 | 3.770177987094108E-4 | 5.796724270012828E-4 | 2.3560372101316736E-4 | 8.83830496296367E-4 | 9.250007180063524E-4 | 0.005848845621532349 | 0.004454939209055925 | 0.040165447842301326 | 0.038261616013568304 | 0.00538286612103089 | 0.029073152002790075 |
| Average | ||||||||||||||||
| Acyl-CoA chain length | C0 | C2:0 | C4:0 | C6:0 | C8:0 | C8:1 | C10:0 | C10:1 | C12:0 | C12:1 | C14:0 | C14:1 | C16:0 | C16:1 | C18:0 | C18:1 |
| WT | 0.01207824049232085 | 0.010282016044658976 | 0.002066175107208663 | 6.447739108716855E-4 | 3.9415493862793186E-4 | 9.432631969506913E-5 | 5.521086063383004E-4 | 2.447629579965277E-4 | 0.00303895884349099 | 0.0019119627717082573 | 0.010864758584281115 | 0.007401716800189686 | 0.04724019127804889 | 0.04350235852484628 | 0.005622850087330942 | 0.03134896072541431 |
| KO4 | 0.006547910229658453 | 0.010455006108217218 | 0.0012324660170634687 | 0.0011830195900333668 | 0.014618183479421648 | 6.171993631851781E-4 | 0.00125739977532814 | 4.460450657963014E-4 | 0.0026230378393230468 | 0.0024840034547059945 | 0.007262400564321443 | 0.006791371239334351 | 0.03353913792549491 | 0.04786706669944211 | 0.0054008120536033054 | 0.03625964543425058 |
| KO9 | 0.0059713046688026 | 0.010230350696444564 | 0.001978413459285073 | 0.001968084833947996 | 0.014422602549533143 | 8.282486491284211E-4 | 9.138659339735396E-4 | 5.154022456742675E-4 | 0.001190550074153618 | 0.003303180369207498 | 0.004912814548881346 | 0.0058234100358517 | 0.03884931702084101 | 0.05774350801996405 | 0.006922096397340832 | 0.04161377565724687 |
| KO21 | 0.00734705715194605 | 0.011279892052502322 | 0.0010554810360987237 | 8.305634717728237E-4 | 0.006474939285948697 | 3.0337746363468877E-4 | 6.301747290749601E-4 | 2.6538894843798063E-4 | 0.0010036908110386468 | 0.0010103775022259076 | 0.004650821998633295 | 0.0038019399314426204 | 0.03762184411281377 | 0.03446365990502759 | 0.00548279306917138 | 0.027144624925773037 |
| Replicates vs. mean WT | ||||||||||||||||
| Acyl-CoA chain length | C0 | C2:0 | C4:0 | C6:0 | C8:0 | C8:1 | C10:0 | C10:1 | C12:0 | C12:1 | C14:0 | C14:1 | C16:0 | C16:1 | C18:0 | C18:1 |
| WT_1 | 1.1347046453733292 | 1.1041853752458963 | 1.0214811670776034 | 1.0662220099742348 | 0.9126861094129449 | 1.2323730715078185 | 0.7922601576464795 | 0.9501410572969642 | 0.9546527520800855 | 1.0443633640706151 | 1.0075432826555226 | 0.9986651992808152 | 1.043563516324789 | 0.953921682944739 | 1.1255810029146844 | 1.0522693082564478 |
| WT_2 | 1.0761138468560254 | 0.9970265982730656 | 1.0625818357189654 | 1.3579699466449684 | 1.3106218372209588 | 1.4451396996545702 | 1.4515522117005446 | 1.270969837114682 | 1.1718322244795585 | 1.2535007087444272 | 1.1319816128637714 | 0.9922949990587012 | 1.024938178623794 | 0.9083694979451278 | 1.0782267546489936 | 0.9947412044636169 |
| WT_3 | 1.0508507553041448 | 0.9414674052387473 | 0.959195129607198 | 0.8277250771643437 | 0.7883469721425276 | 0.5589992411596082 | 0.7753179172054105 | 0.7651696117178844 | 1.0404952222612067 | 1.1121453073849985 | 0.9861628512091367 | 0.9507896801530153 | 1.0203200914447692 | 1.0961599958994848 | 0.90277948709149 | 0.9912560006747123 |
| WT_4 | 0.9457759798855349 | 1.0004920964649253 | 1.0725949927843983 | 0.9548002887379097 | 1.0381633033958186 | 0.7875500060880332 | 0.9325722246229098 | 0.8673862862822412 | 0.8534903646814863 | 0.8820163707147188 | 0.8858921365271175 | 0.9060224550668617 | 1.0337352138725044 | 0.9726874581749043 | 0.9899119788058958 | 0.9518008350537798 |
| WT_5 | 0.792554772580966 | 0.9568285247773648 | 0.8841468748118344 | 0.7932826774785434 | 0.9501817778277493 | 0.9759379815899694 | 1.0482974888246552 | 1.1463332075882278 | 0.9795294364976632 | 0.7079742490852401 | 0.988420116744452 | 1.1522276664406064 | 0.8774429997341435 | 1.068861365035745 | 0.9035007765389358 | 1.0099326515514444 |
| KO4_1 | 0.4474654194899084 | 0.9355722313080805 | 0.4448510776090812 | 1.4924322470555786 | 43.02465469063477 | 6.0185661898696985 | 2.7080088502653865 | 1.8847229004050068 | 1.0308705768567488 | 1.743915163545544 | 0.5916320626305759 | 0.9939444241182819 | 0.653256254482344 | 1.1151358400048268 | 0.9464398182269255 | 1.1415930220044335 |
| KO4_2 | 0.5746258643375782 | 1.0356018824375908 | 0.7775044886228244 | 1.522273017690228 | 32.82976104287966 | 5.436436011027095 | 2.1823096799917265 | 1.662837529085207 | 0.7156145679087877 | 1.209611570221256 | 0.6855353059681826 | 0.8235745433980449 | 0.7360092073562001 | 1.0998371416961727 | 0.9904199334613206 | 1.127082370918089 |
| KO4_3 | 0.712869703467299 | 1.0740961656543575 | 0.7172038066452722 | 2.2542349109336413 | 44.44000875255486 | 8.291947263647165 | 2.346472031677681 | 2.084613259169306 | 0.9176817327678951 | 1.498786679023107 | 0.6518263061480339 | 0.9326167773825074 | 0.7825267757018181 | 1.1299772442667666 | 1.1300948124479018 | 1.2631919981967883 |
| KO4_4 | 0.44111216090305433 | 1.0737962954616427 | 0.5900064739763088 | 2.078802144383907 | 32.649390063931605 | 5.336712398382478 | 1.9048576316884214 | 1.7053399263560258 | 0.8516133718199673 | 1.0663775275619691 | 0.7458998459914535 | 0.9704364960306086 | 0.7379056619769703 | 1.1554224479278743 | 0.9009096852185181 | 1.204288443678525 |
| KO4_5 | 0.5345494105355736 | 0.9650560635425455 | 0.4529161624484526 | 1.826167868346828 | 32.49320585507246 | 7.6325177346881405 | 2.245602076459312 | 1.7742627225344603 | 0.7999047835999329 | 0.9772604752885103 | 0.6672886860659252 | 0.8671277634535314 | 0.6401541457557729 | 1.0012907212169406 | 0.8346931456682617 | 1.0470734326488567 |
| KO9_1 | 0.4199833983496997 | 1.0377619022354305 | 0.8245670787450289 | 2.855030045026226 | 39.75761625497305 | 6.595372915147532 | 2.09556372975214 | 2.832240524819353 | 0.41245606151696856 | 2.3777270783058877 | 0.5272897015412172 | 0.9517520390456167 | 0.9720980780809167 | 1.4765806349424533 | 1.5557637957695976 | 1.6851221015898383 |
| KO9_2 | 0.5057060153476535 | 0.9603862939043885 | 1.0204232701667577 | 2.9774755020552823 | 41.23493890099953 | 8.1397386535219 | 1.4984293400440583 | 1.8860772252115654 | 0.4574551138781029 | 1.5754478222209474 | 0.4588405880605139 | 0.6560631314043731 | 0.746460077157578 | 1.2168590049902246 | 0.9206756596826915 | 1.135757333459011 |
| KO9_3 | 0.5325731212496077 | 0.9338259666935032 | 0.9630902890676846 | 3.012500461872734 | 32.76313122965668 | 9.050929682607153 | 1.2178275337763318 | 2.291719494534689 | 0.32720536074434564 | 1.365050086641257 | 0.4707778975127491 | 0.7290168572904303 | 0.789059338074359 | 1.2380326852618757 | 1.178115862203512 | 1.2292734435311385 |
| KO9_4 | 0.5166097213590495 | 1.1354555915015943 | 1.059584661414468 | 3.7305959269260534 | 36.148873101057475 | 9.605367825595158 | 1.921831262708324 | 1.7545309909743494 | 0.4814839279978739 | 1.7798834954261264 | 0.46340769616367683 | 0.8630278431866406 | 0.9080484032609552 | 1.5468727180694124 | 1.3917360592520196 | 1.4781450677577517 |
| KO9_5 | 0.49705429317736005 | 0.9074461124260337 | 0.9199576188732527 | 2.686219306912348 | 33.051445070033544 | 10.511960195136309 | 1.5424909386148111 | 1.7640315553152257 | 0.2802119704299031 | 1.5400836885228142 | 0.34057859991316525 | 0.7339639925463847 | 0.6962265375803615 | 1.1584799448552379 | 1.1090358057759855 | 1.108887895601666 |
| KO21_1 | 0.5286465855776756 | 1.1475462280651805 | 0.5383139774340971 | 1.4222935543182111 | 16.257765895745358 | 2.241773810879862 | 1.1289653425433193 | 1.2645953849743745 | 0.34555163585065674 | 0.5418233774247408 | 0.4342026299857166 | 0.4386244812666122 | 0.8556149963022549 | 0.792836357544982 | 0.9858316301377944 | 0.8537177187141106 |
| KO21_2 | 0.6741100165756884 | 1.1513152054716305 | 0.5179821881910391 | 1.5461255071910056 | 17.660938863531694 | 3.8937084862690403 | 1.1483616675803752 | 1.2489667999147658 | 0.4108356189358759 | 0.5333566644124936 | 0.45892449044294015 | 0.7056015439991947 | 0.9573318825879632 | 0.9089105771993421 | 1.0289132359368691 | 0.9245663181769467 |
| KO21_3 | 0.8706693811201274 | 0.796514306691817 | 0.39954825586051845 | 0.9630126490621412 | 23.901039279195846 | 3.4710899469346157 | 1.7219132354422284 | 0.8197899762209653 | 0.3986644488638227 | 0.5633145124560848 | 0.245414776244987 | 0.2867722014265025 | 0.5389626541788111 | 0.6322401518903406 | 0.9889119076193844 | 0.786423131509045 |
| KO21_4 | 0.5137831197561376 | 1.1202834548650344 | 0.4462920297894499 | 0.9319336464163677 | 10.25199088640199 | 2.4777486872208687 | 0.6578166973175817 | 1.1254149256654098 | 0.20548780077650852 | 0.5199610674319867 | 0.4634508431204705 | 0.5354050877095843 | 0.7798257270824802 | 0.7476086717884889 | 0.9144806268504058 | 0.837318613577894 |
| KO21_5 | 0.45423437403029165 | 1.269593893167027 | 0.652054218277209 | 1.5773688117534126 | 14.065245376427487 | 3.99695228148628 | 1.0499246350202571 | 0.962579153895132 | 0.2908333221390622 | 0.48379640633897053 | 0.5383318530422135 | 0.6018791760503127 | 0.8502388909878317 | 0.8795296924353023 | 0.9573198711378116 | 0.9274040137228774 |
| Mean Clone vs. mean WT (for WT, this should be 1 everywhere) | ||||||||||||||||
| Acyl-CoA chain length | C0 | C2:0 | C4:0 | C6:0 | C8:0 | C8:1 | C10:0 | C10:1 | C12:0 | C12:1 | C14:0 | C14:1 | C16:0 | C16:1 | C18:0 | C18:1 |
| WT | 1.0000000000000002 | 0.9999999999999998 | 1 | 0.9999999999999998 | 1 | 0.9999999999999998 | 1 | 1 | 1 | 1 | 1 | 1 | 1.0000000000000002 | 1.0000000000000002 | 0.9999999999999998 | 1.0000000000000004 |
| KO4 | 0.5421245117466827 | 1.0168245276808432 | 0.5964964018603879 | 1.8347820376820365 | 37.08740408101467 | 6.543235919522916 | 2.2774500540165055 | 1.8223552675100012 | 0.8631370065906664 | 1.2991902831280773 | 0.6684364413608341 | 0.9175400008765948 | 0.7099704090546212 | 1.100332679022516 | 0.9605114790045854 | 1.1566458534893385 |
| KO9 | 0.4943853098966741 | 0.9949751733521902 | 0.9575245836534384 | 3.0523642485585287 | 36.59120091134405 | 8.780673854401611 | 1.655228560979133 | 2.1057199581710364 | 0.39176248691343885 | 1.7276384342234068 | 0.4521788966382645 | 0.7867647726946891 | 0.8223784868308341 | 1.3273649976238406 | 1.231065436536761 | 1.3274371683878812 |
| KO21 | 0.6082886954119842 | 1.097050617652138 | 0.5108381339104627 | 1.2881468337482276 | 16.42739606026047 | 3.216254642558133 | 1.1413963155807523 | 1.0842692481341294 | 0.3302745653131852 | 0.5284504056128553 | 0.42806491856726553 | 0.5136564980904413 | 0.7963948302278682 | 0.7922250901716912 | 0.9750914543364531 | 0.8658859591401746 |
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| * The data in this sheet are relative means of each clone (WT, KO4, KO9, KO21) to the mean of the WT values in each biological replicate (N1, N2 ). | |||||||||||
| MCAD-KO (average for of biological replicate) vs WT | |||||||||||
| KO4 | KO9 | KO21 | |||||||||
| Acylcarnitine chain length | N=1 | N=2 | N=1 | N=2 | N=1 | N=2 | |||||
| Carnitine | 0.32958519352061255 | 0.5421245117466827 | 0.7861861599175755 | 0.4943853098966741 | 0.3589435431063106 | 0.6082886954119842 | |||||
| C2:0 | 1.1400100159776103 | 1.0168245276808432 | 0.8642348715148886 | 0.9949751733521902 | 0.6226514974202496 | 1.097050617652138 | |||||
| C4:0 | 0.4894726141441003 | 0.5964964018603879 | 0.6372714012266562 | 0.9575245836534384 | 0.8291060663455884 | 0.5108381339104627 | |||||
| C6:0 | 2.8923832211202516 | 1.8347820376820365 | 1.9755684587299889 | 3.0523642485585287 | 5.406237506731751 | 1.2881468337482276 | |||||
| C8:0 | 10.121947735173668 | 37.08740408101467 | 17.710323528334065 | 36.59120091134405 | 30.550320681540917 | 16.42739606026047 | |||||
| C10:0 | 1.227242931759221 | 2.2774500540165055 | 1.6772823392275953 | 1.655228560979133 | 2.137264311950173 | 1.1413963155807523 | |||||
| C12:0 | 0.6049754663263657 | 0.8631370065906664 | 0.5929926237067557 | 0.39176248691343885 | 0.8986832490030056 | 0.3302745653131852 | |||||
| C14:0 | 0.7458861678750642 | 0.6684364413608341 | 0.6537600820328365 | 0.4521788966382645 | 0.7057352571912762 | 0.42806491856726553 | |||||
| C16:0 | 0.8043923214938007 | 0.7099704090546212 | 0.7627461059177327 | 0.8223784868308341 | 0.7398809508663773 | 0.7963948302278682 | |||||
| C8:0/C10:0 | 8.247713205945393 | 16.284617972458896 | 10.558939967429598 | 22.106433983774973 | 14.294123806177677 | 14.392368221288738 | |||||
| MCAD KO (average of each clone) vs WT | |||||||||||
| Acylcarnitine chain length | KO4 | KO9 | KO21 | ||||||||
| Carnitine | 0.43585485263364765 | 0.6402857349071248 | 0.4836161192591474 | ||||||||
| C2:0 | 1.0784172718292266 | 0.9296050224335395 | 0.8598510575361937 | ||||||||
| C4:0 | 0.5429845080022441 | 0.7973979924400473 | 0.6699721001280255 | ||||||||
| C6:0 | 2.363582629401144 | 2.5139663536442587 | 3.347192170239989 | ||||||||
| C8:0 | 23.60467590809417 | 27.15076221983906 | 23.488858370900694 | ||||||||
| C10:0 | 1.7523464928878631 | 1.6662554501033642 | 1.6393303137654627 | ||||||||
| C12:0 | 0.734056236458516 | 0.49237755531009725 | 0.6144789071580954 | ||||||||
| C14:0 | 0.7071613046179492 | 0.5529694893355506 | 0.5669000878792708 | ||||||||
| C16:0 | 0.757181365274211 | 0.7925622963742834 | 0.7681378905471228 | ||||||||
| C8:0/C10:0 | 13.47032450710916 | 16.294477667367747 | 14.3283255202839 | ||||||||
