HepG2 CoA labelling data, palmitate/no-glucose (Fig. 3, Fig. S8, Tables S1-S4)

Selected Cell

Cell:

Value:

Note on data sheet Exp1_MeOH Exp1_NH3 Exp2_MeOH Exp2_NH3 Exp3_MeOH Exp3_NH3 COMBINED

The sheets: "Exp1_", "Exp2_" and "Exp3" contain the integrated peak areas as obtained from the HILIC-MS/MS measurements, as well as the area ratio. Suffixes "NH4" and "MeOH" indicate experiments in which ammonia was used to release CoA from acylated states (yielding a total CoA) and experiments in which a methanol control was used (yielding only an already -free CoA fraction). These data were used to calculate fractional labelling of the total and free CoA pools.
The data used for plotting, were:
Fig. 3, Fig. S8, Tables S1-S4: The sheet "COMBINED" contains the fractional label enrichment of the freen and total CoA pools calculated from measure data.

		Area		Area ratio				Area ratio
Sample #	Sample Name	CoA	Labeled CoA	CoA	Labeled CoA	Protein (mg/mL)	Average Fraction	Labeled fraction	labeled CoA/mg protein	Sum	CoASH/mg protein		Average CoA
1	HepG2 WT_ T0H_n1	2292318.06552765	7167.65948600303	0.7713197476221	0.00241177583029361	3.650016061676839	0.3771353286465375	0.31170706597713055	6.60757593813334E-4	0.7737315234523936	0.21198030649129163	0.21198030649129163	0.1508735017168247
2	HepG2 WT_ T0H_n2	1721200.29492582	5356.7000899009	0.586266826243747	0.001824572985552	3.758432380340508		0.3102533021130942	4.854611712840492E-4	0.5880913992292991	0.15647252357271862	0.15647252357271862
3	HepG2 WT_ T0H_n3	3696049.14698721	10838.5466006124	7.74181523578109	0.0227026270131562	3.7102473498233217		0.29238939769772293	0.006118898518784005	7.7645178627942455	2.0927224334959726	Outlier
4	HepG2 WT_ T0H_n4	2297102.40310168	10804.2337481043	0.642017995867723	0.00301967926570371	3.9029874718920654		0.46813998346361496	7.73684078529683E-4	0.6450376751334267	0.16526767758768374	0.16526767758768374
5	HepG2 WT_ T0H_n5	1249029.74611556	6316.73898708923	0.253392904649376	0.0012814881669779	3.650016061676839		0.5031868939811248	3.510911035249463E-4	0.2546743928163539	0.06977349921560481	0.06977349921560481
6	HepG2 WT_ T24H_n1	3672930.77617482	932123.372482518	6.07435450896403	1.54156126417571	2.959363957597173	29.38397669553698	20.2413118802151	0.5209096570289266	7.61591577313974	2.57349750901319	Outlier	0.12806083755264677
7	HepG2 WT_ T24H_n2	1048342.99632041	382393.727271232	0.303231984411895	0.11060693795266	2.622068743976871		26.72705054437224	0.04218308090004663	0.413838922364555	0.1578291657360931	0.1578291657360931
8	HepG2 WT_ T24H_n3	1090778.62924675	312379.659518088	0.544857564803919	0.156037546038868	3.0356569225827172		22.262610143084263	0.05140157468984086	0.700895110842787	0.23088745820672976	0.23088745820672976
9	HepG2 WT_ T24H_n4	437702.056381204	309034.998690359	0.0879490563069044	0.0620955193250256	3.1199807259877934		41.384714551327946	0.01990253298932352	0.15004457563193	0.048091507226995935	0.048091507226995935
10	HepG2 WT_ T24H_n5	622990.317935187	355080.892914723	0.144895803149892	0.0825851215996139	3.01557982653389		36.30419635868536	0.02738615004416491	0.2274809247495059	0.07543521904076825	0.07543521904076825
11	HepG2 MCAD KO9_ T0H_n1	1551952.44902262	8489.77640602378	0.278013338023585	0.0015208398164512	3.1199807259877934	0.5819484166694586	0.5440622067050072	4.8745167038482216E-4	0.2795341778400362	0.08959484124746797	Variable
12	HepG2 MCAD KO9_ T0H_n2	725135.825787124	6610.24384711119	0.17467210771537	0.00159228820894919	3.2364278830709923		0.9033521492524528	4.919893989537299E-4	0.1762643959243192	0.0544626366761693
13	HepG2 MCAD KO9_ T0H_n3	2676541.82441391	10200.8473675993	3.21544246783274	0.0122547077480625	3.4934147124959845		0.37967340433221863	0.0035079453075603276	3.2276971755808024	0.9239375915018886
14	HepG2 MCAD KO9_ T0H_n4	2055555.76403898	6273.80422045181	2.99482522794287	0.00914056795893728	3.6379698040475428		0.30428335673486695	0.002512546406725983	3.003965795901807	0.8257258739640022
15	HepG2 MCAD KO9_ T0H_n5	904214.164771886	7093.3531332904	0.178174231652814	0.0013977360603336	3.4291680051397364		0.7783709663227478	4.0760209422187327E-4	0.17957196771314762	0.05236604547925326
16	HepG2 MCAD KO9_ T24H_n1	62930.8027206645	234083.010122781	0.00963661032199997	0.035845192720119	2.871024734982332	59.81243664293608	78.81216293673346	0.01248515635666914	0.04548180304211897	0.015841661859593438	0.015841661859593438	0.023738029715983246
17	HepG2 MCAD KO9_ T24H_n2	353478.859747636	186734.671577196	0.0558264333341404	0.0294918080855518	2.714423385801477		34.56682603252161	0.010864851901813346	0.0853182414196922	0.031431442075680695	0.031431442075680695
18	HepG2 MCAD KO9_ T24H_n3	125367.167796605	324753.253706352	0.0221608135167393	0.0574057500127693	2.5096370061034365		72.1480826446393	0.02287412477308811	0.0795665635295086	0.03170441117022212	0.03170441117022212
19	HepG2 MCAD KO9_ T24H_n4	234987.392097252	206978.12349526	0.249250874397165	0.219541473275839	2.686315451333119		46.83128348097	0.0817258722042077	0.46879234767300404	0.17451127991701784	Outlier
20	HepG2 MCAD KO9_ T24H_n5	91584.431699608	183475.512215789	0.0145873362965516	0.0292235148398614	2.742531320269836		66.70382811981604	0.010655672233849318	0.043810851136413004	0.01597460375843674	0.01597460375843674
21	HepG2 MCAD KO21_ T0H_n1	1305085.55506195	6026.05262827421	0.278270323814698	0.00128487485720021	4.280436877610023	0.6457468310901874	0.459614009435114	3.001737658884993E-4	0.2795551986718982	0.06530996874038417	0.06530996874038417	0.06116644606180528
22	HepG2 MCAD KO21_ T0H_n2	895910.037967108	10277.3000166771	0.22155220217914	0.00254150344862429	4.284452296819787		1.134125316686007	5.931921451222056E-4	0.2240937056277643	0.052303932942397775	0.052303932942397775
23	HepG2 MCAD KO21_ T0H_n3	1453480.15982333	6937.23953170756	0.257153049874654	0.00122735235925508	4.308544812078382		0.47501758981858494	2.848647078740774E-4	0.25838040223390907	0.059969296712332434	0.059969296712332434
24	HepG2 MCAD KO21_ T0H_n4	2020656.8628558	8324.32332615896	1.17323761576267	0.00483328437972264	4.412945711532283		0.4102710948159778	0.0010952512665387865	1.1780709001423928	0.26695794082935537	Outlier
25	HepG2 MCAD KO21_ T0H_n5	1284378.91334291	9701.80264507823	0.257988176030014	0.00194876320578354	3.8748795374237073		0.749706144695253	5.029222681537127E-4	0.2599369392357975	0.06708258585210675	0.06708258585210675
26	HepG2 MCAD KO21_ T24H_n1	27515.3045915259	53115.523411396	0.00426661689753065	0.00823627406901548	2.895117250240925	52.159128565728984	65.87495716833173	0.0028448844579023794	0.01250289096654613	0.004318612990719346	0.02077546426933993
27	HepG2 MCAD KO21_ T24H_n2	280011.426302192	121778.520825364	0.043452318409987	0.0188976540431969	2.8188242852553804		30.309001431213773	0.0067040908303671745	0.0623499724531839	0.022119141224702165
28	HepG2 MCAD KO21_ T24H_n3	36221.5244708409	88850.9260642911	0.00555064425425503	0.0136156578015101	2.903148088660456		71.03956601484623	0.00468996323497659	0.01916630205576513	0.006601902993039761
29	HepG2 MCAD KO21_ T24H_n4	28230.6611135657	72606.9411637327	0.00573324589423259	0.0147454374392857	2.557822036620623		72.00383539869111	0.005764841035917914	0.02047868333351829	0.008006297170140338
30	HepG2 MCAD KO21_ T24H_n5	488585.506095316	134358.276897949	0.14979420202761	0.0411925663423314	3.039672341792483		21.568282815562064	0.013551646924563027	0.1909867683699414	0.06283136696809803

		Area			Area ratio				Area ratio
Sample #	Sample name	CoA	Labeled CoA		CoA	Labeled CoA	Protein (mg/mL)	Average Fraction	Label Fraction	Label CoA/mg protein	Sum	CoA/mg protein	Average CoA
31	HepG2 WT_ T0H_n1	801021.078269134	2303.17422815312		0.22294266880152	6.41026588524821E-4	2.144233858014777	0.18590939239930265	0.2867054269796111	2.989536734198903E-4	0.22358369539004483	0.10427206647928232	0.11239061461252135
32	HepG2 WT_ T0H_n2	1245107.30718881	1374.35840663135		0.307527107956403	3.3945063501488E-4	2.517667844522968		0.1102590149992155	1.3482741011819097E-4	0.30786655859141787	0.12228243660543336
33	HepG2 WT_ T0H_n3	1165087.32252801	2215.40907183837		0.257650931676979	4.89922257643539E-4	2.1924188885319627		0.18978873362200083	2.2346197627023254E-4	0.25814085393462255	0.11774248766278096
34	HepG2 WT_ T0H_n4	1111714.63833618	2530.50426887295		0.277034367129562	6.30590463120159E-4	2.0237712817218116		0.2271048059457317	3.115917637608024E-4	0.2776649575926821	0.13720174809301897
35	HepG2 WT_ T0H_n5	803811.597617109	930.998504699814		0.161019384252039	1.86497440955985E-4	2.0036941856729844		0.11568898044995407	9.307679898933567E-5	0.16120588169299496	0.08045433422209111
36	HepG2 WT_ T24H_n1	1323789.7188782	5717.63468276535		0.329888214961283	0.00142483377261501	1.6945069065210405	0.8265883914241904	0.43005664221797657	8.408545088437018E-4	0.331313048733898	0.1955218048736729	0.1680306506348453
37	HepG2 WT_ T24H_n2	1184955.22111838	11901.8703542223		0.333906574698992	0.00335380839010864	1.8069386443944746		0.9944270238294189	0.0018560720921614573	0.3372603830891006	0.18664739067668806
38	HepG2 WT_ T24H_n3	1014121.08144779	9830.74556316783		0.25607268977487	0.00248233224306793	1.6704143912624474		0.9600789122926846	0.0014860577447443207	0.25855502201793795	0.15478495837343098
39	HepG2 WT_ T24H_n4	926260.88300411	7929.39614728389		0.253447881187659	0.00216967885560355	1.7587536138772886		0.8487988287018849	0.001233645712784265	0.2556175600432625	0.14534017614879932
40	HepG2 WT_ T24H_n5	973853.608997288	8840.12166790972		0.256920541549691	0.00233218712268575	1.642306456794089		0.8995805500789869	0.001420068168786453	0.25925272867237675	0.15785892310163502
41	HepG2 MCAD KO9_ T0H_n1	987360.445805241	2147.57077233584		0.258593526019289	5.62457105460993E-4	1.8062669478758664	0.14275778791878085	0.2170341964245691	3.113920155171035E-4	0.25915598312474997	0.14347601467740534	0.15603996688066407
42	HepG2 MCAD KO9_ T0H_n2	841746.497104318	1326.11659392222		0.208960442293644	3.29203520242967E-4	1.5501657125640254		0.15729565548393865	2.1236666349590039E-4	0.20928964581388695	0.13501114372328296
43	HepG2 MCAD KO9_ T0H_n3	1239041.06271833	1014.83650266106		0.268857550003591	2.20207759023796E-4	1.6217234106658631		0.08183796418359751	1.3578626143984746E-4	0.26907775776261483	0.16592086911548884
44	HepG2 MCAD KO9_ T0H_n4	1773332.77387635	3511.83237247936		0.314049881356163	6.21930951802721E-4	1.8702922567038263		0.197644316229394	3.325314263444593E-4	0.31467181230796576	0.16824740153848383
45	HepG2 MCAD KO9_ T0H_n5	1291138.40141719	774.84831992149		0.287944007078657	1.72803264058651E-4	1.7196444712262733		0.05997680727240492	1.004877850916623E-4	0.2881168103427157	0.16754440534865933
46	HepG2 MCAD KO9_ T24H_n1	1360142.03703419	14328.1554423309		0.33503422813093	0.00352935382368499	1.463543235914432	1.1005634782018054	1.0424493394443428	0.0024115131941967022	0.33856358195461494	0.23133145208592218	0.24188046710179384
47	HepG2 MCAD KO9_ T24H_n2	1392444.36811296	13617.2299238192		0.368787517376845	0.00360650991318284	1.3693883699909615		0.96846610012196	0.002633664774885334	0.37239402729002785	0.2719418650331358
48	HepG2 MCAD KO9_ T24H_n3	1289056.51438806	15510.8614554089		0.279537676688915	0.00336359975400067	1.4409460680927988		1.188965916411969	0.002334299547000152	0.2829012764429157	0.19633023241276265
49	HepG2 MCAD KO9_ T24H_n4	1326861.11426883	11791.5658535389		0.360366430630796	0.0032025088779012	1.3354926182585116		0.8808532660212549	0.0023979981874233686	0.3635689395086972	0.2722358285909455
50	HepG2 MCAD KO9_ T24H_n5	1382280.17846596	19940.7420947401		0.325981597959304	0.00470260304228027	1.3919855378125945		1.4220827690095001	0.0033783418825385743	0.33068420100158424	0.237562957386203
51	HepG2 MCAD KO21_ T0H_n1	1195818.13556782	968.807822094633		0.244491075669915	1.98077667076751E-4	2.4691172039771017	0.1647008558015605	0.08095073458526121	8.022205942986413E-5	0.24468915333699173	0.09909985356015565	0.12896384875594954
52	HepG2 MCAD KO21_ T0H_n2	1323720.95859197	3062.26508900616		0.327074599939586	7.56646725576534E-4	2.164055438385056		0.2308037239504968	3.496429491386726E-4	0.3278312466651625	0.15148930145237371
53	HepG2 MCAD KO21_ T0H_n3	1062367.65244417	1646.18839387416		0.221744639826204	3.4360369656011E-4	2.363663754142814		0.15471494173210953	1.453691101189299E-4	0.2220882435227641	0.09395932189318727
54	HepG2 MCAD KO21_ T0H_n4	1622009.45257479	833.76893333037		0.379901354993941	1.95282430087722E-4	2.457818620066285		0.05137704753485323	7.94535562931228E-5	0.3800966374240287	0.15464796072452974
55	HepG2 MCAD KO21_ T0H_n5	1312923.74714504	4025.35807308445		0.322920867791086	9.9005911421502E-4	2.2243145525760775		0.30565783120508155	4.45107511016546E-4	0.323910926905301	0.14562280614950138
56	HepG2 MCAD KO21_ T24H_n1	1187856.42345211	14758.4610569192		0.267651592127162	0.00332542344448616	1.5878276589334137	0.9969194523582399	1.227197604738139	0.0020943226588708854	0.27097701557164816	0.17065895914275148	0.20421360725923104
57	HepG2 MCAD KO21_ T24H_n2	1278655.20934108	13373.0467318713		0.255113991341981	0.0026681558118616	1.5162699608315757		1.0350429001079207	0.0017596838826763339	0.25778214715384257	0.17001071960329925
58	HepG2 MCAD KO21_ T24H_n3	1635484.77040258	15967.006708818		0.425083465405583	0.00415002980569964	1.5012051822838204		0.9668466818175178	0.0027644654139723245	0.4292334952112826	0.28592593489337625
59	HepG2 MCAD KO21_ T24H_n4	1211729.81979687	8922.41667778943		0.2716123768106	0.00199998280239995	1.2940644772521845		0.7309548462023957	0.0015455047546368878	0.27361235961299996	0.2114364194541436
60	HepG2 MCAD KO21_ T24H_n5	1227494.99784158	12706.549805631		0.299933123514299	0.00310479079669928	1.655619162398313		1.0245552289252264	0.0018753049416278269	0.3030379143109983	0.18303600320258476
61	K33F Pre-mat (Control) - ORGANOIDS	1202851.24229741	2276.76117965867	The labelled CoA intensity in organoids are too low for quantification	0.348252095013841	6.59172824353514E-4	0.40569448629105154		NA		0.348252095013841	0.8584097314154759
62	BB2 Pre-mat (Control) - ORGANOIDS	2224022.45357793	1241.66995839496		0.76629038827487	4.27819311354976E-4	0.7017173847544441		NA		0.76629038827487	1.0920213820027023
63	BB3 Pre-mat (MCADD) - ORGANOIDS	1851512.05980651	1950.54982907154		0.577139805990577	6.08011135527222E-4	0.5819523952997891		NA		0.577139805990577	0.9917302697813746
64	BB4 Pre-mat (MCADD) - ORGANOIDS	1510669.13062497	1396.97277655871		0.459085599095501	4.24533785092464E-4	0.6226272973787286		NA		0.459085599095501	0.7373361255252687
65	K33F Pre-mat (Control) - ORGANOIDS	2173078.39848694	2305.08427263158		0.779413355973727	8.26759618974159E-4	0.5164206086170534		NA		0.779413355973727	1.5092607517367558
66	BB1 Pre-mat (Control) - ORGANOIDS	1216745.94939255	853.298545989268		0.304230527807174	2.13355521876207E-4	0.34844832780958124		NA		0.304230527807174	0.8731008402870821
67	BB2 Pre-mat (Control) - ORGANOIDS	1580331.21585469	1552.3973298936		0.478961492934417	4.70495384317972E-4	2.8933680104031207		NA		0.478961492934417	0.16553770250182773
68	BB3 Pre-mat (MCADD) - ORGANOIDS	2015498.00104079	N/A		0.736433658696094	N/A	0.8026514010244051		NA		0.736433658696094	0.9175012436983241
69	BB4 Pre-mat (MCADD) - ORGANOIDS	1759716.60340078	4252.39036913415		0.532147435383903	0.00128594492136559	0.7604700210906901		NA		0.532147435383903	0.6997612274323193
70	BB3 Pre-mat (MCADD) - ORGANOIDS	1858160.7837272	4066.23182367722		0.616644056392459	0.00134940835472532	0.6738475444410968		NA		0.616644056392459	0.9151091541098015

			Area		Area Ratio				Area Ratio
Sample #	Sample name	Component Name	CoA	Labeled CoA	CoA	Labeled CoA	Protein (mg/mL)	Average Fraction	Sum	Fraction	CoA/ mg protein	Average CoA
1	HepG2 WT_ T0H_n1	CoA_ CE40	2401163.07751992	11337.691759101	1.32823987946885	0.00627161664967688	3.1673487949067765	0.5528451481411095	1.3345114961185267	0.46995598523640264	0.4213339239001636	Outlier	0.1474519608087101
2	HepG2 WT_ T0H_n2	CoA_ CE40	1457152.07023888	8523.52325143568	0.407577915075712	0.00238410246045905	2.765651053509171		0.40996201753617106	0.5815422791572881	0.1482334573683815	0.1482334573683815
3	HepG2 WT_ T0H_n3	CoA_ CE40	1263313.74636198	7022.61336495138	0.253740028430348	0.00141051114183596	2.477641352129756		0.2551505395721839	0.5528152690568449	0.10298122420053211	0.10298122420053211
4	HepG2 WT_ T0H_n4	CoA_ CE40	1100227.73661128	8187.48610230034	0.530793635563999	0.00394996905618383	3.57662573897226		0.5347436046201828	0.7386659741334188	0.1495106403763232	0.1495106403763232
5	HepG2 WT_ T0H_n5	CoA_ CE40	2041937.78458329	8637.97313670003	0.731224463941189	0.00309328586018152	3.883583447021373		0.7343177498013705	0.4212462331215933	0.1890825212896035	0.1890825212896035
6	HepG2 WT_ T24H_n1	CoA_ CE40	573395.32617277	188890.72945257	0.174437209071597	0.0574639697277133	2.875549492193421	26.60357234008095	0.2319011787993103	24.77950738553305	0.080645865921932	0.080645865921932	0.05954125432481764
7	HepG2 WT_ T24H_n2	CoA_ CE40	1370672.83035184	286051.890609437	0.659426466813348	0.137618681404402	2.9475519175382754		0.7970451482177501	17.266108665503733	0.2704091973665465	Outlier
8	HepG2 WT_ T24H_n3	CoA_ CE40	321073.052490125	172499.480119523	0.0694517756370872	0.0373136116465077	2.856601485523723		0.10676538728359489	34.949165264013565	0.03737496736056649	0.03737496736056649
9	HepG2 WT_ T24H_n4	CoA_ CE40	503779.874391136	166652.088715218	0.151769529427753	0.0502058743673105	2.9740791268758526		0.2019754037950635	24.857419974885865	0.06791191329439521	0.06791191329439521
10	HepG2 WT_ T24H_n5	CoA_ CE40	489471.8827596	221614.888288669	0.101615500421157	0.0460077658542283	2.826284674852206		0.1476232662753853	31.165660410468533	0.05223227072237687	0.05223227072237687
11	HepG2 MCAD KO4_ T0H_n1	CoA_ CE40	1848598.93317509	11867.9793816206	0.824583351834522	0.00529381362413282	3.6410489616492354	0.42756573513376217	0.8298771654586549	0.6379032758669827	0.22792255039678372	0.2987375771205182
12	HepG2 MCAD KO4_ T0H_n2	CoA_ CE40	2030412.24234432	9424.19338669839	0.983016471364715	0.00456268787945966	4.15264514173109		0.9875791592441747	0.46200730713592897	0.23781929963620446
13	HepG2 MCAD KO4_ T0H_n3	CoA_ CE40	3162860.55690747	7300.5736422859	2.03453455392748	0.00469615054836495	3.5842049416401394		2.039230704475845	0.23029030202701015	0.56894924751225
14	HepG2 MCAD KO4_ T0H_n4	CoA_ CE40	1630577.16228506	8317.95534046221	0.608109365573157	0.00310210805225913	3.997271487039564		0.6112114736254161	0.50753432913471	0.1529071707056075
15	HepG2 MCAD KO4_ T0H_n5	CoA_ CE40	2148811.53695432	6467.85252497457	1.19787852985637	0.00360557524040743	3.9252690616947095		1.2014841050967773	0.30009346150417926	0.3060896173517451
16	HepG2 MCAD KO4_ T24H_n1	CoA_ CE40	1318328.13201618	327283.634300683	0.779388767206616	0.193488390386089	3.23935122025163	21.26449219116673	0.972877157592705	19.888265324767026	0.30033086610384063
17	HepG2 MCAD KO4_ T24H_n2	CoA_ CE40	1051776.96813862	271450.777798761	0.41485258100745	0.107068379701821	2.955131120206154		0.521920960709271	20.514290048117456	0.17661516172347067
18	HepG2 MCAD KO4_ T24H_n3	CoA_ CE40	1849415.1502467	367439.309016085	1.21903795580469	0.242196817780748	2.9892804937469544		1.461234773585438	16.57480523724936	0.4888249117612357
19	HepG2 MCAD KO4_ T24H_n4	CoA_ CE40	534495.231987831	184050.106075152	0.144690049585168	0.0498231179259155	2.871528341724866		0.1945131675110835	25.61426486619552	0.06773855047317541
20	HepG2 MCAD KO4_ T24H_n5	CoA_ CE40	153323.674034514	47706.0278095536	0.0302307647318346	0.00940617756574455	3.0915567682279828		0.03963694229757915	23.730835479504275	0.012821030072916382
21	HepG2 MCAD KO9_ T0H_n1	CoA_ CE40	700677.606626327	10710.2858829591	0.19380014446031	0.00296235377254698	2.856601485523723	1.3298941295893174	0.19676249823285696	1.5055479571321597	0.06887992575442597	0.05262737066823336
22	HepG2 MCAD KO9_ T0H_n2	CoA_ CE40	503394.494451365	5518.49865457541	0.104303192897934	0.00114343131682935	2.7732302561770505		0.10544662421476335	1.0843697703404152	0.038023032519529584
23	HepG2 MCAD KO9_ T0H_n3	CoA_ CE40	212442.167970597	4399.77404507405	0.0433347485627298	8.97482377427524E-4	2.8831286948613006		0.044232230940157324	2.029023538608636	0.015341746977508825
24	HepG2 MCAD KO9_ T0H_n4	CoA_ CE40	825320.093761052	5823.28349803494	0.253027889735048	0.00178531111259156	2.8869182961952404		0.25481320084763953	0.7006352522760588	0.08826477742146904
25	HepG2 MCAD KO9_ T24H_n1	CoA_ CE40	120814.542686023	63492.8970204558	0.024054855176927	0.0126417930212246	2.530695770804911	31.173186092203952	0.036696648198151605	34.449448769714515	0.014500616242180662	0.016858419414176973
26	HepG2 MCAD KO9_ T24H_n2	CoA_ CE40	126105.634157665	44461.690231414	0.0268609752816127	0.00947050756504317	2.602698196149765		0.03633148284665587	26.06694476252263	0.013959160881734932
27	HepG2 MCAD KO9_ T24H_n3	CoA_ CE40	172031.545337602	68514.5662316703	0.0463001601599273	0.0184398470849573	2.750492648173412		0.0647400072448846	28.482924036766022	0.023537604177156446
28	HepG2 MCAD KO9_ T24H_n4	CoA_ CE40	167457.765478306	67629.3594577357	0.0307888300418893	0.0124343523170672	2.54585417614067		0.0432231823589565	28.767785337514862	0.01697787043894231
29	HepG2 MCAD KO9_ T24H_n5	CoA_ CE40	121667.988106277	74884.0048521786	0.0248004623488571	0.015264146073048	2.6157219425044658		0.040064608421905096	38.09882755450173	0.015316845330870521
30	HepG2 MCAD KO21_ T0H_n1	CoA_ CE40	236653.703986314	4468.30374485982	0.0456384920724335	8.61709077871874E-4	4.012506090628552	2.1144534456135204	0.04650020115030537	1.8531297855820459	0.011588817586821705	0.028569641605113306
31	HepG2 MCAD KO21_ T0H_n2	CoA_ CE40	579592.372089037	6260.31877625347	0.136679456831706	0.00147630819716897	3.6751553736546914		0.13815576502887494	1.0685824054178392	0.037591816122725843
32	HepG2 MCAD KO21_ T0H_n3	CoA_ CE40	885606.720198903	5461.26617591135	0.26907062248675	0.00165927635371632	3.7357889949977263		0.27072989884046633	0.6128899544612492	0.07246926933051558
33	HepG2 MCAD KO21_ T0H_n4	CoA_ CE40	349444.405018199	4847.56171980235	0.0592502771505009	8.21931532678252E-4	4.076853115052296		0.060072208683179154	1.368239241898228	0.014734945554301278
34	HepG2 MCAD KO21_ T0H_n5	CoA_ CE40	133266.103302775	8009.51649546202	0.0238858575667277	0.00143558013213835	3.917689859026831		0.02532143769886605	5.66942584070824	0.00646335943120214
35	HepG2 MCAD KO21_ T24H_n1	CoA_ CE40	151499.479952303	57172.3109222077	0.0315856521857794	0.0119196760808329	2.89070789752918	25.471058798295548	0.0435053282666123	27.39819823398627	0.015050060334286384	0.014483555933865303
36	HepG2 MCAD KO21_ T24H_n2	CoA_ CE40	131750.122386232	43716.8787946902	0.025105518511824	0.00833042801009158	2.77701985751099		0.03343594652191558	24.914587073619714	0.012040225939141763
37	HepG2 MCAD KO21_ T24H_n3	CoA_ CE40	170944.475121482	66909.5014950156	0.0344952419469857	0.0135018077711054	3.2848264362589057		0.0479970497180911	28.130495208367535	0.01461174605400309
38	HepG2 MCAD KO21_ T24H_n4	CoA_ CE40	164500.736692843	66604.6440732639	0.0333869046320342	0.0135180118000128	3.1749279975746547		0.046904916432047	28.82003173291413	0.014773537059069664
39	HepG2 MCAD KO21_ T24H_n5	CoA_ CE40	128385.861932839	28358.0865403918	0.035124040669066	0.00775825756782054	2.6898590268303773		0.04288229823688654	18.09198174259008	0.015942210282825612

		Area		Area Ratio				Area Ratio
Sample #	Sample name	CoA	Labeled CoA	CoA	Labeled CoA	Protein (mg/mL)	Average Fraction	Sum	Fraction	CoA/ mg protein	Average CoA
40	HepG2 WT_ T0H_n1	1275536.94440493	1154.93089650698	0.537643312388318	4.86807438625235E-4	1.6590874639987874	0.07772775260206866	0.5381301198269433	0.09046277483627695	0.32435307450875694	0.28959292016578136
41	HepG2 WT_ T0H_n2	1276016.26936322	766.895225947686	0.52800102922259	3.1733252806278E-4	1.7230169050715214		0.5283183617506527	0.06006464114009908	0.3066240152349071
42	HepG2 WT_ T0H_n3	1058514.99252642	756.034258213732	0.497494107078921	3.55330430713503E-4	1.600202282907094		0.4978494375096345	0.07137307063978084	0.31111656496651746
43	HepG2 WT_ T0H_n4	1137301.0694335	831.490543923539	0.412580985506736	3.01641489023118E-4	1.7410778789192314		0.4128826269957591	0.07305744279383697	0.23714196360478412
44	HepG2 WT_ T0H_n5	1233062.66551589	1156.22654653708	0.472288735106846	4.42858897955918E-4	1.7591388527669414		0.4727315940048019	0.09368083360034944	0.2687289825139412
45	HepG2 WT_ T24H_n1	1043508.18071742	13097.4085219828	0.424969163784777	0.00533392535888697	1.4304291287386215	1.1302086136494425	0.430303089143664	1.2395740336193934	0.3008209777740706	0.3263721225681052
46	HepG2 WT_ T24H_n2	854293.538450261	11527.7017116379	0.316750482576601	0.00427418084746876	1.4448779078167893		0.32102466342406977	1.3314182162454657	0.22218116955579872
47	HepG2 WT_ T24H_n3	1214452.78868768	12218.268137856	0.510367584936345	0.00513466481340999	1.473775465973125		0.515502249749755	0.9960509029596976	0.3497834382860838
48	HepG2 WT_ T24H_n4	1372110.33158743	10393.6168198057	0.511862826799445	0.00387731654195842	1.3437364542696142		0.5157401433414034	0.7517965378529321	0.38381048731890893
49	HepG2 WT_ T24H_n5	1242549.27565948	16776.7843054688	0.513588560770135	0.00693442479472179	1.3870827915041182		0.5205229855648569	1.332203377569724	0.37526453990566394
50	HepG2 MCAD KO4_ T0H_n1	1249530.245435	1633.69989897027	0.531175310235409	6.94485830845165E-4	1.9505851755526658	0.11150758211736074	0.5318697960662542	0.13057440674045234	0.27267191544996633	0.24613251341819486
51	HepG2 MCAD KO4_ T0H_n2	1216288.24690424	1519.1219842441	0.464933116094713	5.8069303856243E-4	2.1167461349515966		0.46551380913327545	0.12474238726528927	0.2199195271680136
52	HepG2 MCAD KO4_ T0H_n3	1094477.67591449	1163.16591932814	0.464000489420035	4.93120661775041E-4	1.9252998121658715		0.46449361008181006	0.10616306684782789	0.24125780678245465
53	HepG2 MCAD KO4_ T0H_n4	1058468.02937746	806.218099234906	0.475765745121171	3.6238312737539E-4	2.1022973558734286		0.4761281282485464	0.0761104219379831	0.22647991584936011
54	HepG2 MCAD KO4_ T0H_n5	1242489.44899646	1492.12638988591	0.521799204183337	6.26637403973355E-4	1.9325242017049553		0.5224258415873104	0.11994762779525105	0.27033340184117954
55	HepG2 MCAD KO4_ T24H_n1	1184571.3155283	14935.7808409033	0.469811815162529	0.0059236672505488	1.6905071521456436	1.0087518780516471	0.47573548241307784	1.2451598565871362	0.2814158353658899	0.3312367070964437
56	HepG2 MCAD KO4_ T24H_n2	1485406.62192389	15557.2655697295	0.575766063824554	0.00603023133783726	1.6543852044502239		0.5817962951623913	1.0364850013618767	0.35166918417632403
57	HepG2 MCAD KO4_ T24H_n3	1264295.17848316	10944.6108948099	0.565636881892921	0.00489654290028922	1.6724461782979338		0.5705334247932102	0.8582394453162796	0.34113709140335274
58	HepG2 MCAD KO4_ T24H_n4	1876590.41054047	14956.4856315187	0.562457517870337	0.00448280441865018	1.4376535182777053		0.5669403222889873	0.7907012859045071	0.3943511528203097
59	HepG2 MCAD KO4_ T24H_n5	1155072.27047096	13002.7045995553	0.469494155366699	0.00528511848956586	1.6507730096806819		0.47477927385626484	1.1131738010884362	0.2876102717163422
60	HepG2 MCAD KO9_ T0H_n1	1353970.17684632	685.88564912822	0.525640103964416	2.66275439504291E-4	1.5387949718248806	0.28997445020274765	0.5259063794039203	0.05063171886336432	0.3417650752915054	0.3385163977102782
61	HepG2 MCAD KO9_ T0H_n2	1273900.74392303	1075.13317608848	0.483588690546283	4.08134030274897E-4	1.5279583875162548		0.4839968245765579	0.08432576611054583	0.3167604749781898
62	HepG2 MCAD KO9_ T0H_n3	1593867.94056468	1332.60866819898	0.603170542964117	5.04301688677857E-4	1.4809998555122088		0.6036748446527949	0.08353862897300407	0.4076130341309263
63	HepG2 MCAD KO9_ T0H_n4	1156044.01983092	398.005304942164	0.454344739678987	1.5642277764756E-4	1.5785291142898425		0.4545011624566345	0.03441636470236417	0.2879270064404913
64	HepG2 MCAD KO9_ T24H_n1	1264700.09828132	15321.3417143889	0.443044599815371	0.00536731017710102	1.3545730385782404	1.5999081369938453	0.448411909992472	1.1969597723644598	0.33103560843284247	0.33141763076730896
65	HepG2 MCAD KO9_ T24H_n2	1002811.11192196	22845.0077997092	0.35882963655411	0.00817448644953132	1.3617974281173237		0.3670041230036413	2.227355481085485	0.2694997915446368
66	HepG2 MCAD KO9_ T24H_n3	1402602.98848083	19639.6037921779	0.526430388876462	0.00737121220089085	1.4412657130472473		0.5338016010773529	1.3808898635773654	0.37037001313848217
67	HepG2 MCAD KO9_ T24H_n4	1088724.03508756	20407.4213408562	0.406705912053326	0.00762343683210958	1.3112267013437364		0.4143293488854356	1.8399461328571014	0.31598605219130577
68	HepG2 MCAD KO9_ T24H_n5	1208765.72753093	16596.1721102954	0.484113862211114	0.00664681070553423	1.3256754804219044		0.49076067291664827	1.3543894350848154	0.3701966885292777
69	HepG2 MCAD KO21_ T0H_n1	1408082.52406895	507.85085881407	0.522906095161239	1.88595700157801E-4	2.293743678659153	0.10304761271302558	0.5230946908613968	0.03605383565396819	0.22805280979223483	0.20217116108087868
70	HepG2 MCAD KO21_ T0H_n2	1213101.89754052	1192.64735076893	0.500942993127894	4.92496413410552E-4	2.2901314838896107		0.5014354895413046	0.09821730286004891	0.2189548910482883
71	HepG2 MCAD KO21_ T0H_n3	1030612.52072137	1369.13480974758	0.437740555798034	5.81523919544321E-4	2.1564802774165583		0.4383220797175783	0.13267046002314353	0.2032580980720509
72	HepG2 MCAD KO21_ T0H_n4	1032682.67094915	1881.21064465157	0.443961605638482	8.08753087311987E-4	2.3768241583586183		0.444770358725794	0.1818361029338721	0.18712800320615325
73	HepG2 MCAD KO21_ T0H_n5	1121234.53144411	745.672105903404	0.391977483749375	2.6068290583029E-4	2.2612339257332756		0.39223816665520533	0.06646036209409519	0.1734620032856662
74	HepG2 MCAD KO21_ T24H_n1	1552698.08490218	17676.0083158786	0.531194002780928	0.00604714445248513	1.4557144921254153	0.9476090423693835	0.5372411472334131	1.1255922007511183	0.369056672953097	0.335071366563694
75	HepG2 MCAD KO21_ T24H_n2	1445124.35016339	16539.6466425015	0.513637357553481	0.00587865009358	1.4809998555122088		0.5195160076470611	1.1315628406148603	0.3507873452610059
76	HepG2 MCAD KO21_ T24H_n3	1137671.30528983	12358.2536019709	0.415839220594453	0.00451716284119777	1.643548620141598		0.4203563834356508	1.0746031270604621	0.2557614531655507
77	HepG2 MCAD KO21_ T24H_n4	1146827.30303007	9415.94678838292	0.449982479813147	0.00369455023823589	1.5098974136685448		0.4536770300513829	0.8143569088823935	0.30046877751058576
78	HepG2 MCAD KO21_ T24H_n5	1357461.56592593	8083.07020180849	0.536222175758586	0.00319296074321389	1.3509608438086982		0.5394151365017998	0.5919301345380834	0.3992825839282307

		Area		Area ratio			Area ratio				Remove outlier
Sample #	Sample name	CoA	labelled CoA	CoA	labelled CoA	Protein (mg/mL)	Sum	Sum/protein	Fraction		Sum/protein	Fraction	Average CoA	Average Fraction
1	HepG2 WT_ T0H_n1	1399536.06937317	1705.27836304517	0.640322490138123	7.80207178435068E-4	4.348880597014926	0.6411026973165581	0.14741786604962467	0.12169769082250861	HepG2 WT_ T0H_n1	0.14741786604962467	0.12169769082250861	0.16527744255550905	0.18777524496342818
2	HepG2 WT_ T0H_n2	2688731.11973042	6768.67195823068	0.76386783241202	0.00192297799475021	4.465485074626867	0.7657908104067702	0.1714910693035424	0.2511100901992763	HepG2 WT_ T0H_n2	0.1714910693035424	0.2511100901992763
3	HepG2 WT_ T0H_n3	1138170.10338805	2089.26933302843	5.47150509611278	0.0100437076749675	3.92910447761194	5.481548803787747	1.3951140355319243	0.1832275518194293	HepG2 WT_ T0H_n3		0.1832275518194293
4	HepG2 WT_ T0H_n4	2548537.44416899	3608.47742032195	0.804201423597527	0.00113866982220804	4.292910447761195	0.805340093419735	0.187597692339408	0.14138993345940087	HepG2 WT_ T0H_n4	0.187597692339408	0.14138993345940087
5	HepG2 WT_ T0H_n5	1228701.82084129	2973.89281644185	0.598791644612702	0.00144928748395591	3.882462686567165	0.6002409320966579	0.15460314252946109	0.24145095851652593	HepG2 WT_ T0H_n5	0.15460314252946109	0.24145095851652593
6	HepG2 WT_ T24H_n1	1644602.17259738	528730.155666483	9.25007074156441	2.97384463221833	3.957089552238807	12.223915373782742	3.0891176993623515	24.3280858978825	HepG2 WT_ T24H_n1		24.3280858978825	0.20319364880239127	31.458797218122264
7	HepG2 WT_ T24H_n2	1344866.03373791	552201.333659008	1.08674051650605	0.446215122920464	4.059701492537313	1.532955639426514	0.37760304353520757	29.10815625998122	HepG2 WT_ T24H_n2	0.37760304353520757	29.10815625998122
8	HepG2 WT_ T24H_n3	721180.101618196	384224.765496881	0.393443485758669	0.209615782122525	3.8451492537313436	0.603059267881194	0.1568363743737603	34.75873654325804	HepG2 WT_ T24H_n3	0.1568363743737603	34.75873654325804
9	HepG2 WT_ T24H_n4	337155.894243559	227479.073071688	0.156822387609744	0.105808060839011	4.190298507462686	0.262630448448755	0.06267583275535739	40.28781181465198	HepG2 WT_ T24H_n4	0.06267583275535739	40.28781181465198
10	HepG2 WT_ T24H_n5	1018097.25336831	412039.495786666	0.591759866456431	0.239494248898915	3.8544776119402986	0.831254115355346	0.2156593445452398	28.811195574837612	HepG2 WT_ T24H_n5	0.2156593445452398	28.811195574837612
11	HepG2 MCAD KO4_ T0H_n1	771471.481024478	5616.31424853512	0.513058414178665	0.00373506649663313	3.2574626865671643	0.5167934806752982	0.15864908685106519	0.7227387024605049	HepG2 MCAD KO4_ T0H_n1	0.15864908685106519	0.7227387024605049	0.12818905536812916	0.39296225918091443
12	HepG2 MCAD KO4_ T0H_n2	734456.059665565	2816.63154876883	0.413637862053514	0.00158629701081877	3.476679104477612	0.41522415906433274	0.11943125798684322	0.38203389089722906	HepG2 MCAD KO4_ T0H_n2	0.11943125798684322	0.38203389089722906
13	HepG2 MCAD KO4_ T0H_n3	848844.365799018	1522.39842455047	0.456873041809998	8.1939967689649E-4	3.2481343283582094	0.4576924414868945	0.1409093329333575	0.17902844850016003	HepG2 MCAD KO4_ T0H_n3	0.1409093329333575	0.17902844850016003
14	HepG2 MCAD KO4_ T0H_n4	1548828.75882022	6507.70021684993	0.43631931846325	0.00183327905503381	3.523320895522388	0.43815259751828384	0.12435784605231673	0.4184110890629396	HepG2 MCAD KO4_ T0H_n4	0.12435784605231673	0.4184110890629396
15	HepG2 MCAD KO4_ T0H_n5	1332791.78027061	3509.1149926315	0.37066030636685	9.75913610437486E-4	3.807835820895523	0.37163621997728746	0.09759775301706324	0.26259916498373836	HepG2 MCAD KO4_ T0H_n5	0.09759775301706324	0.26259916498373836
16	HepG2 MCAD KO4_ T24H_n1	301537.060840278	85648.5439594493	0.183885588606906	0.052230836486299	2.7304104477611943	0.236116425093205	0.08647653149980038	22.120797596220303	HepG2 MCAD KO4_ T24H_n1	0.08647653149980038	22.120797596220303	0.07933219178910964	25.226271989147314
17	HepG2 MCAD KO4_ T24H_n2	293976.765063834	99723.4691560326	0.188331839107854	0.0638863562713634	2.707089552238806	0.2522181953792174	0.09316950566730567	25.329796756062105	HepG2 MCAD KO4_ T24H_n2	0.09316950566730567	25.329796756062105
18	HepG2 MCAD KO4_ T24H_n3	468626.510635973	274296.040623308	0.14395737045957	0.0842609964212548	2.7164179104477615	0.2282183668808248	0.08401445374184209	36.921216102319995	HepG2 MCAD KO4_ T24H_n3	0.08401445374184209	36.921216102319995
19	HepG2 MCAD KO4_ T24H_n4	218341.226645283	66425.565357014	0.128479533576927	0.039087101349475	3.080223880597015	0.167566634926402	0.054400797286827056	23.326303214624254	HepG2 MCAD KO4_ T24H_n4	0.054400797286827056	23.326303214624254
20	HepG2 MCAD KO4_ T24H_n5	262821.580041835	59394.9702609951	0.179236255600952	0.040505547791837	2.7957089552238807	0.21974180339278898	0.07859967074977303	18.43324627650991	HepG2 MCAD KO4_ T24H_n5	0.07859967074977303	18.43324627650991
21	HepG2 MCAD KO9_ T0H_n1	822406.022950292	2001.22025109282	0.604250360051344	0.00147036624674375	2.879664179104478	0.6057207262980878	0.21034422370960482	0.24274656337583406	HepG2 MCAD KO9_ T0H_n1	0.21034422370960482	0.24274656337583406	0.0961628639765906	0.3944985771535798
22	HepG2 MCAD KO9_ T0H_n2	318440.26312564	1189.55026436659	0.153527447918828	5.73509814578045E-4	2.781716417910448	0.15410095773340604	0.05539779567076166	0.37216499041505924	HepG2 MCAD KO9_ T0H_n2	0.05539779567076166	0.37216499041505924
23	HepG2 MCAD KO9_ T0H_n3	917280.295087343	2044.83477409722	0.248560133200081	5.54099337513147E-4	2.9076492537313436	0.24911423253759415	0.08567547554709685	0.22242781228066816	HepG2 MCAD KO9_ T0H_n3	0.08567547554709685	0.22242781228066816
24	HepG2 MCAD KO9_ T0H_n4	407429.658615093	2710.4400633555	0.186427301288189	0.00124021414649178	2.6930970149253732	0.18766751543468077	0.06968464722756418	0.66085712469692	HepG2 MCAD KO9_ T0H_n4	0.06968464722756418	0.66085712469692
25	HepG2 MCAD KO9_ T0H_n5	248476.348127544	1184.13065058294	0.143693915425328	6.84782961596264E-4	2.417910447761194	0.14437869838692427	0.059712177727925475	0.47429639499941756	HepG2 MCAD KO9_ T0H_n5	0.059712177727925475	0.47429639499941756
26	HepG2 MCAD KO9_ T24H_n1	193890.019664157	111100.101586415	0.0947739389725047	0.0543060146459741	2.786380597014926	0.1490799536184788	0.05350308345463986	36.42744267613104	HepG2 MCAD KO9_ T24H_n1	0.05350308345463986	36.42744267613104	0.07539363615831682	42.04595535048487
27	HepG2 MCAD KO9_ T24H_n2	203943.227735631	122152.224618362	0.123720961708943	0.0741029298813547	2.5065298507462686	0.1978238915902977	0.07892341339218427	37.459039596099565	HepG2 MCAD KO9_ T24H_n2	0.07892341339218427	37.459039596099565
28	HepG2 MCAD KO9_ T24H_n3	463887.505665424	495684.383983562	0.128646386320845	0.137464372237591	2.6791044776119404	0.266110758558436	0.09932824971261957	51.65682627123278	HepG2 MCAD KO9_ T24H_n3	0.09932824971261957	51.65682627123278
29	HepG2 MCAD KO9_ T24H_n4	395717.188815904	420815.641350141	0.108538398379954	0.115422470937006	2.786380597014926	0.22396086931696	0.08037698423427556	51.536891819103744	HepG2 MCAD KO9_ T24H_n4	0.08037698423427556	51.536891819103744
30	HepG2 MCAD KO9_ T24H_n5	178066.666142799	88299.1345905596	0.112280538230041	0.055677317781219	2.5904850746268657	0.16795785601126	0.06483644999786486	33.1495763898572	HepG2 MCAD KO9_ T24H_n5	0.06483644999786486	33.1495763898572
31	HepG2 MCAD KO21_ T0H_n1	630302.356504322	3157.06025993146	0.612464132656393	0.00306771211290819	3.905783582089552	0.6155318447693012	0.15759496957074062	0.4983839811014092	HepG2 MCAD KO21_ T0H_n1	0.15759496957074062	0.4983839811014092	0.153837288252671	0.33459335147786273
32	HepG2 MCAD KO21_ T0H_n2	1502291.23637938	4202.77119888151	0.434650785205205	0.00121596782128226	3.915111940298508	0.43586675302648725	0.11132932076349637	0.2789769609264891	HepG2 MCAD KO21_ T0H_n2	0.11132932076349637	0.2789769609264891
33	HepG2 MCAD KO21_ T0H_n3	908573.713844456	1129.48321044313	0.568007578993919	7.06112243951525E-4	3.859141791044776	0.5687136912378705	0.1473679180582541	0.12415952962457977	HepG2 MCAD KO21_ T0H_n3	0.1473679180582541	0.12415952962457977
34	HepG2 MCAD KO21_ T0H_n4	1019478.06231466	3837.84449912199	0.889713920266287	0.00334934492551378	3.915111940298508	0.8930632651918008	0.2281067000918776	0.3750400510309256	HepG2 MCAD KO21_ T0H_n4	0.2281067000918776	0.3750400510309256
35	HepG2 MCAD KO21_ T0H_n5	1558905.61605351	6204.19286253698	0.492417730483891	0.00195974312838061	3.961753731343284	0.4943774736122716	0.12478753277898635	0.3964062347059101	HepG2 MCAD KO21_ T0H_n5	0.12478753277898635	0.3964062347059101
36	HepG2 MCAD KO21_ T24H_n1	237659.566648579	112997.080732114	0.146685265035783	0.0697426447805022	3.322761194029851	0.21642790981628518	0.06513495769878093	32.22442282961714	HepG2 MCAD KO21_ T24H_n1	0.06513495769878093	32.22442282961714	0.09307267458045623	38.24993765583143
37	HepG2 MCAD KO21_ T24H_n2	516427.452221878	392873.800910744	0.195251294240192	0.148538033311074	3.173507462686567	0.343789327551266	0.10833102855230957	43.20612113502097	HepG2 MCAD KO21_ T24H_n2	0.10833102855230957	43.20612113502097
38	HepG2 MCAD KO21_ T24H_n3	275194.353840417	164570.587621299	0.13643453306861	0.0815900143502182	3.206156716417911	0.2180245474188282	0.06800183730956759	37.42239821898708	HepG2 MCAD KO21_ T24H_n3	0.06800183730956759	37.42239821898708
39	HepG2 MCAD KO21_ T24H_n4	366407.702019068	176746.573365588	0.259141275985629	0.125003738446695	3.4207089552238807	0.384145014432324	0.11229982423546532	32.540768134508035	HepG2 MCAD KO21_ T24H_n4	0.11229982423546532	32.540768134508035
40	HepG2 MCAD KO21_ T24H_n5	662886.500269826	561415.78704943	0.209223881299697	0.177197136978342	3.4626865671641793	0.386421018278039	0.1115957251061578	45.85597796102398	HepG2 MCAD KO21_ T24H_n5	0.1115957251061578	45.85597796102398

		Area		Area ratio			Area ratio				Remove outlier
Sample #	Sample name	CoA	Labeled CoA	CoA	Labeled CoA	Protein (mg/mL)	Sum	Sum/protein	Fraction		Sum/protein	Fraction	Average CoA	Average Fraction
41	HepG2 WT_ T0H_n1	786301.23802748	5140.49249371324	0.307558745642585	0.00201068413337067	2.5367647058823524	0.3095694297759557	0.12203316652037677	0.6495099127927004	HepG2 WT_ T0H_n1	0.12203316652037677	0.6495099127927004	0.09668493416525017	0.4101398578186942
42	HepG2 WT_ T0H_n2	426481.734298616	369.665838509516	0.183879135883526	1.59382757769398E-4	2.5807225063938617	0.1840385186412954	0.07131278864168127	0.08660293450853444	HepG2 WT_ T0H_n2	0.07131278864168127	0.087
43	HepG2 WT_ T0H_n3	655494.328262959	3929.00143504751	0.258741649752687	0.00155088498763798	2.3569373401534524	0.260292534740325	0.11043676482437934	0.5958238445774812	HepG2 WT_ T0H_n3	0.11043676482437934	0.5958238445774812
44	HepG2 WT_ T0H_n4	520410.539870928	2704.60661067932	0.199816709746439	0.00103846012465168	2.5687340153452682	0.20085516987109067	0.07819228019374881	0.5170193654055139	HepG2 WT_ T0H_n4	0.07819228019374881	0.5170193654055139
45	HepG2 WT_ T0H_n5	710630.484908386	1433.71396615527	0.248339314145181	5.01030494183301E-4	2.4528452685421995	0.2488403446393643	0.10144967064606472	0.20134616631777583	HepG2 WT_ T0H_n5	0.10144967064606472	0.20134616631777583
46	HepG2 WT_ T24H_n1	793040.506970473	46937.3970023919	0.273568101936365	0.0161915746987891	2.2170716112531967	0.2897596766351541	0.13069477556088857	5.587932346838046	HepG2 WT_ T24H_n1	0.13069477556088857	5.587932346838046	0.11739487900409833	6.172551951918431
47	HepG2 WT_ T24H_n2	626908.022254873	46311.557837768	0.222111516986042	0.0164080375432674	2.1731138107416874	0.2385195545293094	0.10975934778487385	6.879116295369054	HepG2 WT_ T24H_n2	0.10975934778487385	6.879116295369054
48	HepG2 WT_ T24H_n3	627568.937092173	30520.8641053747	0.224121434681231	0.0108998062949103	1.8614130434782608	0.2350212409761413	0.1262595863930219	4.637796247538672	HepG2 WT_ T24H_n3	0.1262595863930219	4.637796247538672
49	HepG2 WT_ T24H_n4	649385.495802172	45076.0531815023	0.231674209243934	0.016081293844979	2.2050831202046033	0.247755503088913	0.11235653695717579	6.490791786452404	HepG2 WT_ T24H_n4	0.11235653695717579	6.490791786452404
50	HepG2 WT_ T24H_n5	630684.431488437	49424.3416445338	0.201052932647859	0.0157557541231385	2.009271099744245	0.21680868677099752	0.10790414832453149	7.267123083393975	HepG2 WT_ T24H_n5	0.10790414832453149	7.267123083393975
51	HepG2 MCAD KO4_ T0H_n1	644779.382341691	1041.08542734669	0.231940021379826	3.74499220803332E-4	2.001278772378517	0.23231452060062932	0.11608303840874895	0.1612035355496059	HepG2 MCAD KO4_ T0H_n1	0.11608303840874895	0.1612035355496059	0.12477517740252372	0.3299092467939978
52	HepG2 MCAD KO4_ T0H_n2	805810.08712561	3344.93890842049	0.283131314780949	0.00117528554945406	2.0132672634271094	0.28430660033040306	0.14121652176792393	0.4133866565490277	HepG2 MCAD KO4_ T0H_n2	0.14121652176792393	0.4133866565490277
53	HepG2 MCAD KO4_ T0H_n3	692315.32422484	2178.20532382845	0.281904940581697	8.86946772521785E-4	1.9293478260869565	0.2827918873542188	0.14657382330472185	0.3136393977988539	HepG2 MCAD KO4_ T0H_n3	0.14657382330472185	0.3136393977988539
54	HepG2 MCAD KO4_ T0H_n4	539508.742861262	2430.14882455254	0.208804501211219	9.40533438788424E-4	2.025255754475703	0.2097450346500074	0.10356471482008262	0.44841749906397166	HepG2 MCAD KO4_ T0H_n4	0.10356471482008262	0.44841749906397166
55	HepG2 MCAD KO4_ T0H_n5	702292.852792418	2204.36577350164	0.258270860877861	8.1066387582936E-4	2.2250639386189257	0.25908152475369034	0.1164377887111413	0.31289914500852994	HepG2 MCAD KO4_ T0H_n5	0.1164377887111413	0.31289914500852994
56	HepG2 MCAD KO4_ T24H_n1	874545.237980275	18599.1530092565	0.321793085489829	0.0068436469314822	1.4338235294117645	0.3286367324213112	0.22920305440665809	2.082435180346385	HepG2 MCAD KO4_ T24H_n1	0.22920305440665809	2.082435180346385	0.1724488970858723	2.6322721758133736
57	HepG2 MCAD KO4_ T24H_n2	767375.301919361	26881.6356964156	0.256439999050402	0.0089832531946493	1.5936700767263423	0.2654232522450513	0.16654843190020477	3.3845012140667827	HepG2 MCAD KO4_ T24H_n2	0.16654843190020477	3.3845012140667827
58	HepG2 MCAD KO4_ T24H_n3	972421.276554739	20794.0475736604	0.323526110120086	0.00691821280278172	1.4338235294117645	0.3304443229228677	0.23046373291030775	2.0936092173072587	HepG2 MCAD KO4_ T24H_n3	0.23046373291030775	2.0936092173072587
59	HepG2 MCAD KO4_ T24H_n4	490711.184286552	19201.1725581306	0.165027260401334	0.00645739694800024	1.6096547314578002	0.17148465734933424	0.10653505624403527	3.7655829085897574	HepG2 MCAD KO4_ T24H_n4	0.10653505624403527	3.7655829085897574
60	HepG2 MCAD KO4_ T24H_n5	584229.720466944	10922.4247529093	0.18988408185402	0.00354996420614852	1.493765984654731	0.19343404606016854	0.12949420996815567	1.8352323587566832	HepG2 MCAD KO4_ T24H_n5	0.12949420996815567	1.8352323587566832
61	HepG2 MCAD KO9_ T0H_n1	603659.283672957	4604.64923387535	0.182412790302251	0.00139142549088896	1.5297314578005112	0.18380421579313996	0.12015456363656048	0.7570150036465548	HepG2 MCAD KO9_ T0H_n1	0.12015456363656048	0.7570150036465548	0.1851191969357616	0.4262554967944201
62	HepG2 MCAD KO9_ T0H_n2	933707.637237197	1904.27058611723	0.33011354539341	6.73257333989066E-4	1.5417199488491042	0.330786802727399	0.2145569971863773	0.20353210238072783	HepG2 MCAD KO9_ T0H_n2	0.2145569971863773	0.20353210238072783
63	HepG2 MCAD KO9_ T0H_n3	917023.654642912	4864.55530874822	0.288877149897308	0.00153241289468832	1.749520460358056	0.2904095627919963	0.16599380765890626	0.5276730146058928	HepG2 MCAD KO9_ T0H_n3	0.16599380765890626	0.5276730146058928
64	HepG2 MCAD KO9_ T0H_n4	745216.049188929	3701.52384337798	0.296176488952156	0.00147112281988232	1.653612531969309	0.2976476117720383	0.17999840108708287	0.4942498315790353	HepG2 MCAD KO9_ T0H_n4	0.17999840108708287	0.4942498315790353
65	HepG2 MCAD KO9_ T0H_n5	985775.794371757	1469.09475193062	0.361358613126433	5.38530206503292E-4	1.4777813299232736	0.3618971433329363	0.244892215109881	0.14880753175988948	HepG2 MCAD KO9_ T0H_n5	0.244892215109881	0.14880753175988948
66	HepG2 MCAD KO9_ T24H_n1	804510.902591688	44108.6549191684	0.234783365117164	0.0128723904167773	1.421835038363171	0.24765575553394129	0.17418037173921722	5.1976948361344	HepG2 MCAD KO9_ T24H_n1	0.17418037173921722	5.1976948361344	0.2115017970358441	4.928009105102914
67	HepG2 MCAD KO9_ T24H_n2	990751.723452175	34968.2556727443	0.37845188828142	0.0133573347146404	1.2499999999999998	0.3918092229960604	0.3134473783968484	3.4091424935075367	HepG2 MCAD KO9_ T24H_n2	0.3134473783968484	3.4091424935075367
68	HepG2 MCAD KO9_ T24H_n3	757201.483624844	41424.174909867	0.237918077447534	0.013015769603654	1.3539002557544755	0.250933847051188	0.18534145775114902	5.186932634479921	HepG2 MCAD KO9_ T24H_n3	0.18534145775114902	5.186932634479921
69	HepG2 MCAD KO9_ T24H_n4	700096.629824856	43907.5665766124	0.243734147030799	0.0152861374156678	1.4857736572890026	0.2590202844464668	0.17433360941335085	5.901521360898309	HepG2 MCAD KO9_ T24H_n4	0.17433360941335085	5.901521360898309
70	HepG2 MCAD KO9_ T24H_n5	859111.521903227	44690.8034469176	0.259346763946571	0.0134911649496372	1.2979539641943734	0.2728379288962082	0.210206167878655	4.944754200494407	HepG2 MCAD KO9_ T24H_n5	0.210206167878655	4.944754200494407
71	HepG2 MCAD KO21_ T0H_n1	807926.298366021	7671.75537834898	0.315115637467757	0.00299221611109126	2.3649296675191813	0.31810785357884824	0.13451049219258362	0.9406294366604155	HepG2 MCAD KO21_ T0H_n1	0.13451049219258362	0.9406294366604155	0.12600072541121846	0.6057286811578063
72	HepG2 MCAD KO21_ T0H_n2	782526.556519629	5470.12568516193	0.301228708716801	0.00210569070523116	2.3849104859335037	0.30333439942203216	0.12718900822950624	0.6941813092228591	HepG2 MCAD KO21_ T0H_n2	0.12718900822950624	0.6941813092228591
73	HepG2 MCAD KO21_ T0H_n3	593361.206901211	432.975865893286	0.236872114181292	1.72845658851259E-4	2.237052429667519	0.23704495984014326	0.10596307743908083	0.07291682513216964	HepG2 MCAD KO21_ T0H_n3	0.10596307743908083	0.07291682513216964
74	HepG2 MCAD KO21_ T0H_n4	993639.486072574	8131.39370478003	0.356447665865059	0.00291696973291074	2.26502557544757	0.3593646355979697	0.1586580917643542	0.8117019439202771	HepG2 MCAD KO21_ T0H_n4	0.1586580917643542	0.8117019439202771
75	HepG2 MCAD KO21_ T0H_n5	714693.390556983	3657.94478469005	0.242305397916167	0.00124016785144597	2.3489450127877234	0.243545565767613	0.10368295743056735	0.5092138908533104	HepG2 MCAD KO21_ T0H_n5	0.10368295743056735	0.5092138908533104
76	HepG2 MCAD KO21_ T24H_n1	427076.1542274	16096.4652591072	0.141762822319319	0.00534302915746709	1.7375319693094626	0.1471058514767861	0.08466368048194793	3.632098318203367	HepG2 MCAD KO21_ T24H_n1	0.08466368048194793	3.632098318203367	0.12970987450128546	4.124715137438827
77	HepG2 MCAD KO21_ T24H_n2	738937.987351057	27254.4943134508	0.267934560327427	0.00988231905223673	1.8534207161125318	0.27781687937966376	0.14989412655447837	3.557134136090982	HepG2 MCAD KO21_ T24H_n2	0.14989412655447837	3.557134136090982
78	HepG2 MCAD KO21_ T24H_n3	356374.608614742	18853.5460442506	0.123066397802353	0.00651067147147683	1.7974744245524295	0.12957706927382984	0.07208840777030498	5.024555276611557	HepG2 MCAD KO21_ T24H_n3	0.07208840777030498	5.024555276611557
79	HepG2 MCAD KO21_ T24H_n4	733172.452920945	30482.998388804	0.291303420381085	0.0121114775367688	1.6935741687979535	0.30341489791785375	0.17915654567004186	3.991721441459295	HepG2 MCAD KO21_ T24H_n4	0.17915654567004186	3.991721441459295
80	HepG2 MCAD KO21_ T24H_n5	745948.282762174	34479.8332665766	0.265311116490319	0.0122634280039921	1.705562659846547	0.2775745444943111	0.16274661202965424	4.418066514828934	HepG2 MCAD KO21_ T24H_n5	0.16274661202965424	4.418066514828934

Area Ratio / mg protein

	Free CoA (MeOH)																Total CoA (NH3)
	Free CoA 0H (area ratio/mg protein)				0H Label incorporation (%)				Free CoA 24H (area ratio/mg protein)				24H Label incorporation (%)				Total CoA 0H (area ratio/mg protein)				0H Label incorporation (%)				Total CoA 24H (area ratio/mg protein)				24H Label incorporation (%)
	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21
Exp. 1	0.1508735017168247		Variable	0.06116644606180528	0.38		0.58	0.65	0.128060837552647		0.023738029715983246	0.02077546426933993	29.38397669553698		59.81243664293608	52.159128565728984	0.11239061461252135		0.15603996688066407	0.12896384875594954	0.19		0.143	0.165	0.1680306506348453		0.24188046710179384	0.20421360725923104	0.8265883914241904		1.1005634782018054	0.9969194523582399
Exp. 2	0.1474519608087101	0.2987375771205182	0.05262737066823336	0.028569641605113306	0.55	0.43	1.33	2.11	0.05954125432481764	Variable	0.016858419414176973	0.014483555933865303	26.60357234008095	21.26449219116673	31.173186092203952	25.471058798295548	0.28959292016578136	0.24613251341819486	0.3385163977102782	0.20217116108087868	0.078	0.122	0.29	0.103	0.3263721225681052	0.3312367070964437	0.33141763076730896	0.335071366563694	1.1302086136494425	1.0087518780516471	1.5999081369938453	0.9476090423693835
Exp. 3	0.16527744255550905	0.12818905536812916	0.0961628639765906	0.153837288252671	0.188	0.393	0.394	0.335	0.20319364880239127	0.07933219178910964	0.07539363615831682	0.09307267458045623	31.458797218122264	25.226271989147314	42.04595535048487	38.24993765583143	0.09668493416525017	0.12477517740252372	0.1851191969357616	0.12600072541121846	0.41	0.33	0.426	0.606	0.11739487900409833	0.1724488970858723	0.2115017970358441	0.12970987450128546	6.172551951918431	2.6322721758133736	4.928009105102914	4.124715137438827

% Control

	Free CoA (MeOH)																Total CoA (NH3)
	Free CoA 0H				0H Label incorporation (%)				Free CoA 24H				24H Label incorporation (%)				Total CoA 0H				0H Label incorporation (%)				Total CoA 24H				24H Label incorporation (%)
	WT	KO4	KO9	KO21					WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21
Exp. 1	1		Variable	0.40541543323233076					1		0.18536525427786868	0.1622312071854047					1		1.3883718619978058	1.1474610153220195					1		1.4395020562494565	1.2153354550951332
Exp. 2	1	2.0259993524811204	0.3569119758027973	0.19375558960641287					1	Variable	0.28313846601565706	0.2432524490473885					1	0.8499258658578152	1.1689387900660344	0.6981219049317334					1	1.014905024638933	1.015459372447323	1.0266543720926211
Exp. 3	1	0.7755992190227434	0.5818269117051103	0.9307821192901395					1	0.3904265328010391	0.37104327129652664	0.4580491325837194					1	1.2905338197703355	1.9146643531800205	1.3032095072419747					1	1.4689643922189486	1.8016271138067312	1.1049023228411625

% 0H

	Free CoA (MeOH)																Total CoA (NH3)
	Free CoA 0H				0H Label incorporation (%)				Free CoA 24H				24H Label incorporation (%)				Total CoA 0H				0H Label incorporation (%)				Total CoA 24H				24H Label incorporation (%)
	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21	WT	KO4	KO9	KO21
Exp. 1	1		Variable	1					0.848796084769114		Variable	0.33965459180589763					1		1	1					1.4950594514866646		1.5501186775230393	1.5834949811840968
Exp. 2	1	1	1	1					0.40380103457600475	Variable	0.32033558203873863	0.5069561646609204					1	1	1	1					1.1270031131329767	1.345765752343541	0.9790297693376592	1.6573648030326567
Exp. 3	1	1	1	1					1.229409444269129	0.6188686823636076	0.7840202864243963	0.6050072491370782					1	1	1	1					1.2142003303581044	1.3820769537321798	1.1425168244935588	1.0294375217123692