samples-publish_KPRF

ID	Title	UID	File_PrimaryData	Link_PrimaryData	Scientist	Type	Parent	Protocol	ExtractionKit	SynthesisKit	Instrument	NormalizationGene	PrimerSequences	QuantificationMethod
ID	Title	UID	File_PrimaryData	Link_PrimaryData	Scientist	Type	Parent	Protocol	ExtractionKit	SynthesisKit	Instrument	NormalizationGene	PrimerSequences	QuantificationMethod
65591	D.PCR-241118SAS-1	D.PCR-241118SAS-1	111224_PCR.xlsx	https://fairdomhub.org/data_files/7661	Ryan Finethy	qRT-PCR	RNA-241112SAS-1; RNA-241112SAS-2; RNA-241112SAS-3; RNA-241112SAS-4; RNA-241112SAS-5; RNA-241112SAS-6; RNA-241112SAS-7; RNA-241112SAS-8; RNA-241112SAS-9; RNA-241112SAS-10; RNA-241112SAS-11; RNA-241112SAS-12; RNA-241112SAS-13; RNA-241112SAS-14; RNA-241112SAS-15; RNA-241112SAS-16; RNA-241112SAS-17; RNA-241112SAS-18; RNA-241112SAS-19; RNA-241112SAS-20	https://fairdomhub.org/sops/799	Direct-zol RNA Miniprep kit (Zymo Research)	iScript cDNA synthesis kit (Bio-Rad Laboratories)	Applied Biosystems ViiA 7 Real-Time PCR System	Unspliced XBP1; beta-actin	spliced XBP1 F; 5’-ggtctgctgagtccgcagcagg-3’; R; 5’-gaaagggaggctggtaaggaac-3’ 57; unspliced XBP1 F; 5’-cagactatgtgcacctctgc-3’; R; 5’-cagggtccaacttgtccagaat-3’ 58; HSPA5 F; 5’- acttggggaccacctattcct-3’; R; 5’- atcgccaatcagacgctcc-3’ 59; Trib3 F; 5’- gcaaagcggctgatgtctg-3’; R; 5’-agagtcgtggaatgggtatctg-3’ 60–62; β-actin F; 5’- cgcagccactgtcgagtc-3’; R; 5’-ccttctgacccattcccacc-3’ 63.	delta-delta CT method