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Figure_6
RawData_productivities
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| Space-time yield [volumetric productivity] | |||||||||
| MEAN | STDEV | ||||||||
| Exp name | Fermentation | CDW [mg/L/h] | PHA [mg/L/h] | Fermentation | CDW [mg/l/h] | PHA [mg/l/h] | |||
| F4, F5 | Batch | 35 | 12.762291666666728 | F4F5 | Batch | 0.117851130197745 | 0.02014075815082735 | ||
| CF6 | Continuous | 210 | 4.06 | CF6 | The repeat of the condition did not result in PHA accumulation. Hence only one set of value was considered [ refer manuscript for discussion] | ||||
| FB4, FB5 | Fed-batch | 300.602150537635 | 61.67397849462367 | FB4 | Fed-batch | 66.087872495414 | 7.33649897026575 | ||
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| Fed-batch [FB4 and FB5] | CF6 [Continuous] | G45 - Flask [optimum C/N ratio study] | ||||||||||||||||||||||||
| Exp name | Type | CDW [g/l] | PHA % | PHA [mg/l] | Time [h] | Productivity | C/N | Varied | ||||||||||||||||||
| CDW [g/h/L] | PHA [mg/h/L] | Na-BA | 1.96 gC /l, varied [reduced in half] for 2 conditions [the ratios are named new] | Volumetric productivity [mg/l/h] | C/N | 30/1 | ||||||||||||||||||||
| FB4 | FB4 Linear feed 1 | 8.683333333333339 | 19.25 | 1671.5416666666679 | 25 | 0.34733333333333355 | 66.86166666666672 | Dilution rate [/h] | Ratio | CDW [g/l] | PHA [%cdw] | BA [mM] | PHA [mg/l] | C/N ratio | CDW [mg/l/h] | PHA [mg/l/h] | Na-BA | 1.96 gC /l | ||||||||
| FB5 | FB5 Linear feed 2 | 7.869999999999999 | 22.25 | 1751.0749999999998 | 31 | 0.25387096774193546 | 56.486290322580636 | 0.2 | 10 | 1.2705882352941213 | 0 | 0 | 0 | 10 | 254.11764705882428 | 0 | Volumetric productivity | |||||||||
| Other fed batches tested | 0.2 | 15 | 0.911764705882357 | 0 | 15.690301413843422 | 0 | 15 | 182.3529411764714 | 0 | Time [h] | C [mg/l] | N [mg/l] | C/N | Biomass [mg/l] | PHA [mg/l] | Biomass [mg/l/h] | PHA [mg/l/h] | |||||||||
| FB1 | FB1 Exponential | 3.7318181818181846 | 0.53 | 19.778636363636377 | 25.5 | 0.1463458110516935 | 0.7756327985739756 | 0.2 | 20 | 0.6470588235294189 | 1.29 | 2.2280493415167566 | 8.347058823529505 | 20 | 129.41176470588377 | 1.669411764705901 | 1 | 1880.5486432508806 | 76.85257383015215 | 24.46955969759689 | 0 | 0 | 0 | 0 | ||
| FB2 | FB2 Int. exponential | 6.05 | 14.88 | 900.2400000000001 | 51 | 0.11862745098039215 | 17.651764705882357 | 0.2 | 25 | 0.6294117647058913 | 1.69 | 5.273846209081361 | 10.637058823529564 | 25 | 125.88235294117828 | 2.1274117647059128 | 2 | 1977.7930205871521 | 77.06390079741075 | 25.6643253212223 | 0 | 0 | 0 | 0 | ||
| FB3 | FB3 Constant | 5.210526315789473 | 14 | 729.4736842105262 | 25 | 0.2084210526315789 | 29.17894736842105 | 0.2 | 30 | 0.5352941176470521 | 2.13 | 9.359924591772032 | 11.401764705882208 | 30 | 107.05882352941043 | 2.280352941176442 | 3 | 1992.2956492502447 | 74.10532325579041 | 26.88464960031831 | 5.999999999999339 | 0 | 1.9999999999997797 | 0 | ||
| FB6 | FB6 Custom 1 | 1.85 | 0.095 | 1.7575 | 7.5 | 0.24666666666666667 | 0.23433333333333334 | 0.2 | 25 | 0.711764705882353 | 2.37 | 7.162036715871874 | 16.868823529411767 | 25 | 142.3529411764706 | 3.3737647058823534 | 4 | 1954.1870051927342 | 70.93541874691145 | 27.548818907590082 | 5.999999999999339 | 0 | 1.4999999999998348 | 0 | ||
| FB7 | FB7 Custom 2 | 1.2071428571428564 | 0.88 | 10.622857142857137 | 22 | 0.05487012987012984 | 0.4828571428571426 | 0.2 | 20 | 0.8000000000000033 | 2.02 | 4.831569832327443 | 16.160000000000068 | 20 | 160.00000000000068 | 3.2320000000000135 | 5 | 1918.6798152566785 | 61.21437825301598 | 31.34361354331238 | -7.999999999999119 | 0 | -1.5999999999998238 | 0 | ||
| FB11 | FB11 Custom 3 | 14.5 | 0.88 | 127.60000000000002 | 73 | 0.19863013698630136 | 1.7479452054794524 | 0.2 | 15 | 1.047058823529414 | 1.94 | 0 | 20.31294117647063 | 15 | 209.41176470588283 | 4.062588235294126 | 6 | 1857.217940340769 | 52.97262652993068 | 35.05995571677762 | 67.99999999999696 | 0 | 11.333333333332826 | 0 | ||
| FB14 | FB14 Custom 4 | 12.35 | 0.94 | 116.09 | 23 | 0.5369565217391304 | 5.047391304347826 | 0.2 | 10 | 1.452941176470585 | 0.54 | 0 | 7.845882352941161 | 10 | 290.58823529411706 | 1.5691764705882323 | 7 | 1713.7376996493126 | 36.4891230837601 | 46.965713473449604 | 148.00000000000148 | 0 | 21.142857142857356 | 0 | ||
| 0.2 | 35 | 0.43428571428571083 | 1.02 | 8.289941046246566 | 4.429714285714251 | 35 | 86.85714285714218 | 0.8859428571428503 | 8 | 1555.9346609517352 | 19.37163873581373 | 80.32023940623918 | 240.00000000000023 | 0 | 30.00000000000003 | 0 | ||||||||||
| 0.2 | 40 | 0.40000000000000036 | 1.15 | 9.829045981250175 | 4.600000000000003 | 40 | 80.00000000000007 | 0.9200000000000007 | 9 | 1405.9357226656357 | 12.186521849021426 | 115.36808780091195 | 304.00000000000205 | 0 | 33.777777777778006 | 0 | ||||||||||
| 0.2 | New 20 [=40] based on the C usage trend | 0.4028571428571429 | 2.21 | 0.8886435525113154 | 8.903142857142857 | New 20 [=40] | 80.57142857142858 | 1.7806285714285714 | 10 | 1238.190786045233 | 3.944770125936136 | 313.8816069165493 | 369.9999999999992 | 0 | 36.99999999999992 | 0 | ||||||||||
| 0.2 | New 15 [=30] | 0.5199999999999998 | 1.78 | 0 | 9.255999999999997 | New 15 [=30] | 103.99999999999997 | 1.8511999999999995 | 11 | 1155.5439952936147 | 0.2613410168808884 | 4421.594471028931 | 424.00000000000216 | 5.681600000000029 | 38.54545454545474 | 0.5165090909090936 | ||||||||||
| 0.2 | 40 | 0.3714285714285749 | 1.16 | 8.961334114677463 | 4.308571428571468 | 40 | 74.28571428571499 | 0.8617142857142936 | 12 | 977.8405267420583 | 0.011975195515743732 | 81655.49576677024 | 496.0000000000031 | 22.07200000000014 | 41.33333333333359 | 1.839333333333345 | ||||||||||
| 13 | 872.1748879923806 | 0.7431665022304901 | 1173.5928427542058 | 529.9999999999993 | 69.27099999999992 | 40.76923076923072 | 5.3285384615384555 | |||||||||||||||||||
| C/N | 35/1 | 14 | 854.7810853147383 | 0.6903347604158407 | 1238.2124359489578 | 546.000000000002 | 75.78480000000029 | 39.00000000000015 | 5.41320000000002 | |||||||||||||||||
| Na-BA | 1.96 gC /l | Volumetric productivity | 15 | 739.1988270958373 | 0.7326001538675602 | 1009.00719607202 | 607.9999999999997 | 113.02719999999994 | 40.53333333333331 | 7.535146666666662 | ||||||||||||||||
| Ratio | Dilution rate | CDW [g/l] | PHA [%cdw] | BA [mM] | PHA [mg/l] | Dilution rate | CDW [g/l/h] | PHA [mg/l/h] | 16 | 629.7266199677566 | 0.7114674571417005 | 885.1095206766937 | 626.0000000000022 | 132.58680000000044 | 39.125000000000135 | 8.286675000000027 | ||||||||||
| 35 | 0.2 | 0.5514285714285742 | 1.05 | 7.958924057488429 | 5.7900000000000285 | 0.2 | 110.28571428571483 | 1.1580000000000057 | 17 | 520.699863724132 | 0.519159916936377 | 1002.9662282035224 | 662.0000000000026 | 165.50000000000065 | 38.94117647058839 | 9.735294117647097 | ||||||||||
| 0.1 | 0.46285714285714263 | 4.84 | 2.867752029121499 | 22.402285714285703 | 0.1 | 46.28571428571426 | 2.2402285714285703 | 18 | 334.0882943185572 | 0.4346291300329381 | 768.6744197132817 | 758.0000000000009 | 222.85200000000026 | 42.111111111111164 | 12.38066666666668 | |||||||||||
| 0.075 | 0.42571428571428643 | 7.015 | 2.042449684237173 | 29.863857142857192 | 0.075 | 31.92857142857148 | 2.239789285714289 | 19 | 162.2297805361098 | 0.16201734226934775 | 1001.311206959616 | 798.0000000000009 | 265.4148000000003 | 42.00000000000005 | 13.969200000000015 | |||||||||||
| 0.052 | 0.4485714285714299 | 8.605 | 0.6139758367501926 | 38.599571428571544 | 0.052 | 23.32571428571435 | 2.0071777142857203 | 20 | 94.17206290031548 | 0.033107892241603466 | 2844.3992209802655 | 828.000000000002 | 292.0356000000007 | 41.400000000000105 | 14.601780000000037 | |||||||||||
| 0.075 | 0.4542857142857118 | 6.29 | 28.57457142857127 | 0.075 | 34.071428571428385 | 2.1430928571428454 | 21 | 2.6383894785312307 | 0.018315004533501652 | 144.05617392587104 | 851.9999999999994 | 294.1103999999998 | 40.57142857142854 | 14.005257142857133 | ||||||||||||
| 0.1 | 0.43999999999999917 | 4.88 | 21.47199999999996 | 0.1 | 43.99999999999992 | 2.147199999999996 | 22 | 0 | 0 | 859.9999999999985 | 303.2359999999995 | 39.09090909090902 | 13.783454545454521 | |||||||||||||
| 0.15 | 0.42571428571428643 | 2.81 | 11.962571428571449 | 0.125 | 53.21428571428581 | 1.4953214285714311 | 23 | 0 | 0 | 853.9999999999992 | 331.1811999999997 | 37.13043478260866 | 14.39918260869564 | |||||||||||||
| Batch [F4,F5] | 24 | 0 | 0 | 854.0000000000036 | 314.35740000000135 | 35.583333333333485 | 13.098225000000056 | |||||||||||||||||||
| C/N | 30/1 | 12 | 1020.2968028662071 | -0.007044231537530002 | -144841.46317881614 | 468.00000000000176 | 27.658800000000102 | 39.00000000000015 | 2.3049000000000084 | |||||||||||||||||
| Na-BA | 1.96 gC /l | |||||||||||||||||||||||||
| Volumetric productivity | ||||||||||||||||||||||||||
| Time[h] | Biomass [g/l] | PHA [g/l] | Biomass [mg/l/h] | PHA [mg/l/h] | ||||||||||||||||||||||
| F4 | 4 | 0.4321428571428571 | 0.003457142857142857 | 108.03571428571428 | 0.8642857142857142 | |||||||||||||||||||||
| 8 | 0.4999999999999983 | 0.03594999999999988 | 62.49999999999979 | 4.493749999999984 | ||||||||||||||||||||||
| 23 | 0.8480000000000043 | 0.30663680000000154 | 36.86956521739149 | 13.332034782608762 | ||||||||||||||||||||||
| 27 | 0.8416666666666665 | 0.29895999999999995 | 31.17283950617283 | 11.07259259259259 | ||||||||||||||||||||||
| 28 | 0.8159036144578314 | 0.28050766265060245 | 29.139414802065406 | 10.018130808950088 | ||||||||||||||||||||||
| F5 | 4 | 0.45600000000000307 | 0.003146400000000021 | 114.00000000000077 | 0.7866000000000053 | |||||||||||||||||||||
| 8 | 0.6320000000000014 | 0.052708800000000125 | 79.00000000000018 | 6.5886000000000156 | ||||||||||||||||||||||
| 24 | 0.8520000000000039 | 0.30595320000000137 | 35.50000000000016 | 12.748050000000056 | ||||||||||||||||||||||
| 28 | 0.8359999999999967 | 0.29753239999999886 | 29.85714285714274 | 10.626157142857101 |
