RawData_BA_paper

Selected Cell

Cell:

Value:

Overall_data PHA_data C_data N_assay

Fig 2	P. putida CA-3 duplicate batch fermentation

Carbon, C	Sodium benzoate [Ba-Na], 23.27 mM
Nitrogen, N	N-lim with a C/N ratio of 30
Description	28 h batch - Biomass, PHA, N and C consumption
EXP name	F4 [16/3/21] and F5 [21/3/21]

		Replicates															Mean
		Time [h]	OD540 nm		DO %		BA-Na [mM]		N [mg/L]		CDW [g/L]		PHA [%]		PHA [g/L]
			F4	F5	F4	F5	F4	F5	F4	F5	F4	F5	F4	F5	F4	F5	Time [h]	CDW [g/L]	PHA [g/L]	BA-Na [mM]	N [mg/L]	OD540 nm	DO %	SD-CDW [g/L]	SD-PHA [g/L]	SD-BA-Na [mM]	SD-N [mg/L]	SD-OD540 nm	SD-DO %
		0	0.144	0.177	100	100	22.80837970608931	22.545792914730622	50.7235566164404	50.83831579430564							0			22.677086310409965	50.780936205373024	0.16049999999999998	100			0.18567690081974392	0.08114699287190823	0.023334523779156287	0
		1	0.244	0.282	76	75	22.79606676786046	22.24546429097482	46.936503746887155	52.3301851065539							1			22.520765529417638	49.63334442672053	0.263	75.5			0.3893347451439452	3.813908664979832	0.02687005768508879	0.7071067811865476
		2	0.501	0.608	56	55	22.021957694864213	21.41059354454491	44.870838545312665	43.14945087733391							2			21.71627561970456	44.01014471132329	0.5545	55.5			0.4322997364651314	1.2172048930786736	0.07566042558696058	0.7071067811865476
		3	0.8	1.19	20	20	20.679044410209244	19.327297930737924	24.4437048852982	27.88648022125569							3			20.003171170473586	26.165092553276946	0.995	20			0.9558291020792125	2.434409786157337	0.27577164466275306	0
		4	1.44	1.68	20	20	16.369516030112568	14.346446744598857	0.11475917786524976	3.3280161580922436	0.4321428571428571	0.45600000000000307	0.8	0.69	0.00345714285714286	0.00314640000000002	4	0.4440714285714301	0.00330177142857144	15.357981387355712	1.7213876679787468	1.56	20	0.016869547494024115	2.197283814909847E-4	1.430526010596968	2.272115800413515	0.16970562748477142	0
		5	2.02	1.77	20	21	13.199202108754488	11.873419868591828	0	3.9018120474184927							5			12.536310988673158	1.9509060237092464	1.895	20.5			0.9374696123957087	2.758997757644983	0.1767766952966369	0.7071067811865476
		6	2.1	1.92	20	20	11.010978847432106	9.567956021525202	0	0.45903671146099906							6			10.289467434478654	0.22951835573049953	2.01	20			1.0203712256057471	0.324587971487645	0.1272792206135787	0
		7	2.4	2.33	20	21	9.144230342910726	8.060691778772133	0	0							7			8.60246106084143	0	2.365	20.5			0.7661774663795337	0	0.049497474683058214	0.7071067811865476
		8	2.32	2.63	20	22	7.808008872510563	6.8917656647421826	0	0	0.4999999999999983	0.6320000000000014	7.19	8.34	0.0359499999999999	0.0527088000000001	8	0.5659999999999998	0.0443294	7.349887268626373		2.4749999999999996	21	0.09333809511662694	0.01185026112454926	0.6478817854291369	0	0.21920310216782976	1.4142135623730951
		9	2.55	2.96	20	21	7.220467580938377	1.422412038002096	0	0							9			4.321439809470236		2.755	20.5			4.099844392106494	0	0.28991378028648457	0.7071067811865476
		10	2.66	2.87	20	20	5.789757693260318	5.160727153308319	0	0							10			5.475242423284319		2.765	20			0.4447917603734939	0	0.14849242404917495	0
		23	4.34	4.45	92	90	0.28453594211445765	0.02757027472981104	0	0							23			0.15605310842213435		4.395	91			0.18170216593981048	0	0.07778174593052045	1.4142135623730951
		24		4.54		94	0	0	0	0	0.8480000000000043	0.8520000000000039	36.16	35.91	0.306636800000002	0.305953200000001	24	0.8500000000000041	0.3062950000000015	0		4.54	94	0.0028284271247458787	4.8337819561983497E-4	0	0
		25	4.52	4.42	95	95	0	0	0	0							25					4.47	95			0	0	0.0707106781186545	0
		26	4.31	4.58	95	95	0	0	0	0							26					4.445	95			0	0	0.19091883092036815	0
		27	4.6	4.5	96	95	0	0	0	0							27					4.55	95.5			0	0	0.0707106781186545	0.7071067811865476
		28	4.54	4.46	96	95	0	0	0	0	0.8159036144578314	0.8359999999999967	34.38	35.59	0.280507662650602	0.297532399999999	28	0.825951807228914	0.2890200313253005			4.5	95.5	0.014210290494204381	0.012038307227678485	0	0	0.05656854249492385	0.7071067811865476


		Productivity
			F4	F5	Mean	SD
		CDW [g/L/h]	0.035333333333333515	0.03550000000000016	0.03541666666666684	1.1785113019774494E-4
		PHA [mg/L/h]	12.776533333333417	12.74805000000004	12.762291666666728	0.02014075815082735



PHA monomer composition		C4 (%)	C5 (%)	C6 (%)	C7 (%)	C8 (%)	C9 (%)	C10 (%)	C12 (%)	Total PHA [% CDW]	PHA [mg/L]	Sum of monomer % [for cross check]
	F4-23	0.39345331592256655	0	3.0254787210614764	0	22.906861884078083	0	69.48065790826952	4.193548170668347	36.161532264052624		100
	F5-23	0	0	3.1255458577652724	0	23.478274226226056	0	70.8564495367059	2.539730379302784	35.90555849592925		100
	Mean	0.19672665796128327	0	3.0755122894133744	0	23.19256805515207	0	70.1685537224877	3.366639274985565	36.03354537999094
	SD	0.2782135077691798	0	0.0707581509371754	0	0.40404954198651916	0	0.9728315899670433	1.169425775121549	0.1810007872459118

Experiment	P. putida CA-3 duplicate batch fermentation
Date	31/3/2021




			GC - peak area data										Mass of PHA monomer										PHA monomer distribution
Sample name	Sample amount [mg]	Methanolysis conversion efficiency, X	Internal standard peak	C4	C5	C6	C7	C8	C9	C10	C11	C12	C4	C5	C6	C7	C8	C9	C10	C11	C12	Total PHA [mg]	C4	C5	C6	C7	C8	C9	C10	C11	C12	Total %PHA
F4-T4	12	0.85	144.9	0	0	0	0	0	0	64.5	0	0	0	0	0	0	0	0	0.08206210526315787	0	0	0.08206210526315787	0	0	0	0	0	0	100	0	0	0.8045304437564497
F4-T8	13.9	0.85	142.6	0	0	0	0	116.7	0	534	0	0	0	0	0	0	0.15966327685834503	0	0.6903559252971136	0	0	0.8500192021554587	0	0	0	0	18.783490590974257	0	81.21650940902573	0	0	7.194407127849841
F4-T23	10.6	0.85	179.9	9.1	0	83	0	688.2	0	2209.1	0	138.6	0.012819315175097273	0	0.09857475769366819	0	0.7463408498054475	0	2.26378687446242	0	0.13663225985450853	3.2581540569911414	0.39345331592256655	0	3.0254787210614764	0	22.906861884078083	0	69.48065790826952	0	4.193548170668347	36.161532264052624
F4-T27	9.9	0.85	173.2	0	0	72.7	0	622.5	0	1957.1	0	112.3	0	0	0.08968200566869622	0	0.7012052771362587	0	2.0831303888416186	0	0.11498814107842154	2.989005812724995	0	0	3.0003958268296436	0	23.45948188360962	0	69.6930859074672	0	3.8470363820935494	35.51997400742715
F4-T28a	9.3	0.85	172.3	0	0	74.5	0	604.9	0	1904.7	0	67.9	0	0	0.09238251569672347	0	0.6849391883923388	0	2.037945799920579	0	0.06988849388074389	2.885155997890385	0	0	3.2019937834998595	0	23.74010933527212	0	70.63554973840988	0	2.4223471428181385	36.497862085899875
F4-T28b	12.5	0.85	189.6	11.4	0	102.5	0	860.9	0	2648.9	0	162.2	0.015237759493670887	0	0.11550594073647873	0	0.8858661	0	2.575600825116589	0	0.15171680957622452	3.743927434922963	0.40699932780573317	0	3.085154366482945	0	23.66141212398333	0	68.79409042738527	0	4.052343754342726	35.236964093392594
F4-T28c	11.1	0.85	181.7	0	0	76.5	0	654.2	0	2033.3	0	109.8	0	0	0.08995499774853655	0	0.7024401391304348	0	2.0629934950033304	0	0.10716885664377498	2.962557488526077	0	0	3.0363966976819987	0	23.710599434811673	0	69.63555991717497	0	3.617443950331351	31.399655416280623
F5-T4	12	0.85	144.4	0	0	0	0	0	0	55.2	0	0	0	0	0	0	0	0	0.07047307304271759	0	0	0.07047307304271759	0	0	0	0	0	0	100	0	0	0.6909124808109568
F5-T8	10.3	0.85	149.6	0	0	0	0	112.2	0	473.6	0	0	0	0	0	0	0.14632379999999998	0	0.5836216920911905	0	0	0.7299454920911905	0	0	0	0	20.045852955513567	0	79.95414704448642	0	0	8.337469926798292
F5-T24	9.8	0.85	173.7	0	0	76	0	625.2	0	1996.8	0	74.4	0	0	0.09348298319987439	0	0.7022194569948187	0	2.1192689479138256	0	0.07596163460238793	2.9909330227109066	0	0	3.1255458577652724	0	23.478274226226056	0	70.8564495367059	0	2.539730379302784	35.90555849592925
F5-T28	11.5	0.85	174.4	9.6	0	82.2	0	727.6	0	2282.5	0	135.4	0.013950165137614677	0	0.10070339824854044	0	0.8139541045871558	0	2.4127683697489126	0	0.1376871366174711	3.4790631743396947	0.4009747578171625	0	2.8945550339899575	0	23.39578397399005	0	69.35109392507198	0	3.957592309130842	35.591439123679734

Experiment	P. putida CA-3 duplicate batch fermentation
Date	Mar-april,2021


	Molar Concentration [mM]	BA-Na conc [g/L]	Peak area		RT=5.064 min
	10	1.441	7426.4		y=5184.8
	5	0.7205	3787.7
	2.5	0.36025	1919.1
	1.25	0.180125	968.4
	0.625	0.0900625	487.5
	0.3125	0.04503125	243.3
	0.15625	0.022515625	125.2
	0.078125	0.0112578125	62.4
	0.0390625	0.00562890625	36.9















Experiment	Sample	Peak area	BA Concentration	Dilution factor	BA-Na [g/L]	Retention time [min]	5.09
F4 [Replicate 1]	T-(-1)	6946	1.339685233760222	2	2.679370467520444
	T-0	8521	1.643457799722265	2	3.28691559944453
	T-1	8516.4	1.6425705909581854	2	3.2851411819163707
	T-2	8227.2	1.5867921617034408	2	3.1735843234068817
	T-3	7725.5	1.490028544977627	2	2.980057089955254
	T-4	6115.5	1.1795054775497609	2	2.3590109550995217
	T-5	4931.1	0.9510685079463046	2	1.9021370158926092
	T-6	4113.6	0.7933960808517204	2	1.5867921617034408
	T-7	3416.2	0.6588875173584323	2	1.3177750347168646
	T-8	2917	0.5626060793087486	2	1.1252121586174972
	T-9	2697.5	0.5202707915445147	2	1.0405415830890294
	T-10	2163	0.4171809905878722	2	0.8343619811757444
	T-23	106.3	0.020502237309057245	2	0.04100447461811449
	T-25	0	0	2	0
	T-26	0	0	2	0
	T-27	0	0	2	0
	T-28	0	0	2	0
F5 [Replicate 2]	T-(-1)	7222.9	1.393091343928406	2	2.786182687856812
	T-0	8422.9	1.6245371084709148	2	3.2490742169418296
	T-1	8310.7	1.6028969294861906	2	3.205793858972381
	T-2	7998.8	1.5427403178521832	2	3.0854806357043665
	T-3	7220.5	1.392628452399321	2	2.785256904798642
	T-4	5359.7	1.0337332201820706	2	2.067466440364141
	T-5	4435.8	0.8555392686313841	2	1.7110785372627682
	T-6	3574.5	0.6894190711309983	2	1.3788381422619966
	T-7	3011.4	0.580813146119426	2	1.161626292238852
	T-8	2574.7	0.49658617497299795	2	0.9931723499459959
	T-9	531.4	0.10249189939824101	2	0.20498379879648201
	T-10	1928	0.3718561950316309	2	0.7437123900632618
	T-23	10.3	0.0019865761456565345	2	0.003973152291313069
	T-24	0	0	2	0
	T-25	0	0	2	0
	T-26	0	0	2	0
	T-27	0	0	2	0
	T-28	0	0	2	0

Experiment	P. putida CA-3 duplicate batch fermentation
Date	Mar-april,2021					N=OD/871.39
							2021-03-26T0:0:0+0000
							F4 - Batch CA3			F5-Batch CA3
						Sample	OD at 635 nm	N-concentration [diluted]	N [g/l]	OD at 635 nm	N-concentration [diluted]	N [g/l]
						T-[-1]	0.398	4.567415279036941E-4	0.04567415279036941	0.413	4.7395540458348154E-4	0.04739554045834815
						T-0	0.442	5.07235566164404E-4	0.050723556616440395	0.443	5.083831579430565E-4	0.050838315794305644
						T-1	0.409	4.6936503746887155E-4	0.046936503746887154	0.456	5.23301851065539E-4	0.0523301851065539
						T-2	0.391	4.4870838545312664E-4	0.044870838545312666	0.376	4.3149450877333915E-4	0.04314945087733391
						T-3	0.213	2.44437048852982E-4	0.0244437048852982	0.243	2.7886480221255694E-4	0.027886480221255693
						T-4	0.001	1.1475917786524977E-6	1.1475917786524977E-4	0.029	3.3280161580922436E-5	0.0033280161580922438
						T-5	0.018	2.0656652015744957E-5	0.0020656652015744958	0.034	3.901812047418493E-5	0.003901812047418493
						T-6	0.012	1.3771101343829972E-5	0.0013771101343829973	0.004	4.590367114609991E-6	4.5903671146099907E-4
						T-8	0	0	0	0	0	0
						T-9	0	0	0	0	0	0
						T-10	0	0	0	0	0	0
						T-22	0	0	0	0	0	0
						T-23	0	0	0	0	0	0
						T-24	0	0	0
						T-25	0	0	0	0	0	0
						T-26	0	0	0	0	0	0
						T-27	0	0	0	0	0	0
						T-28	0	0	0	0	0	0






				BLANK	No color after incubation
	N-std 1								N=OD/871.39
	Standard solution	N stock 100x diluted [Original stock concentration - 0.25 g/L of NH4Cl]



		N-concentration	OD at 635nm
		6.54625E-4	0.573
		3.273125E-4	0.27
		1.6365625E-4	0.162											865.9772116468687	0.0024398303046206105	slope	intercept
		8.1828125E-5	0.071											20.08130023484712	0.005728808882372953	se(slope)	se(intercept)
		2.045703125E-5	0.027											0.9973185196459676	0.01173913985314421	r2	se(y)
		1.0228515625E-5	0.007											1859.6416679805095	5	F	df
		5.1142578125E-6	0.001											0.25627239154897014	6.890370224583934E-4	ss reg	ss resid

													Factor	871.39