Selected Cell
Cell:
Value:
Overall_data
PHA_data
C_data
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| Fig 1 | Triplicate endpoint flask fermentation | CDW | PHA | PHA% | BA-Na [mM] | ||||||||||||||||||||||||
| I | II | III | CDW [g/L] | SD-CDW | I | II | III | PHA [g/L] | SD-PHA | I | II | III | PHA [%] | SD-%PHA | I | II | III | BA-Na [mM] | SD-BA | ||||||||||
| Carbon, C | Sodium benzoate [Ba-Na], 23.27 mM | P. putida CA-3 | 0.8358585858585834 | 0.906565656565652 | 0.8863636363636397 | 0.8762626262626251 | 0.036419709853171535 | 0.18288585858585804 | 0.18240101010100918 | 0.2073204545454553 | 0.1908691077441075 | 0.01424934658842213 | 21.88 | 20.12 | 23.39 | 21.796666666666667 | 1.6365919874340498 | 0 | 0 | 0 | 0 | 0 | |||||||
| Nitrogen, N | N-lim with a C/N ratio of 30 | P. putida F1 | 0.8409090909090935 | 0.8611111111111115 | 0.835858585858589 | 0.8459595959595981 | 0.013362380358910629 | 0.18937272727272786 | 0.18694722222222232 | 0.17477803030303096 | 0.18369932659932706 | 0.007820672246823585 | 22.52 | 21.71 | 20.91 | 21.713333333333335 | 0.8050051759667963 | 0 | 0 | 0 | 0 | 0 | |||||||
| Description | Endpoint [T48] Biomass, PHA and C consumption | P. putida KT2440 | 0.7878787878787907 | 0.6414141414141437 | 0.845959595959598 | 0.7584175084175108 | 0.10540723876671636 | 0.20870909090909165 | 0.1715606060606065 | 0.19013484848484907 | 0.026267945547169567 | 26.49 | 20.28 | 23.384999999999998 | 4.391133111168463 | 0 | 0 | 0 | 0 | 0 | |||||||||
| Inoculum prep | |||||||||||||||||||||||||||||
| Single colonies in 10 ml full N MSM+BA-Na | |||||||||||||||||||||||||||||
| 18-20 h old | |||||||||||||||||||||||||||||
| 0.005 starting OD | |||||||||||||||||||||||||||||
| MEAN | |||||||||||||||||||||||||||||
| CDW [g/L] | STDEV CDW | PHA (%CDW) | PHA [g/L] | STDEV PHA | CDW - PHA [g/L] | BA-Na [mM] | STDEV BA | STDEV - PHA (%CDW) | |||||||||||||||||||||
| P. putida CA-3 | 0.8762626262626251 | 0.036419709853171535 | 21.796666666666667 | 0.1908691077441075 | 0.01424934658842213 | 0.6853935185185176 | 0 | 0 | 1.6365919874340498 | ||||||||||||||||||||
| P. putida F1 | 0.8459595959595981 | 0.013362380358910629 | 21.713333333333335 | 0.18369932659932706 | 0.007820672246823585 | 0.662260269360271 | 0 | 0 | 0.8050051759667963 | CDW [g/L] Single factor ANOVA | CDW [g/L] Single factor ANOVA | ||||||||||||||||||
| P. putida KT2440 | 0.7584175084175108 | 0.10540723876671636 | 23.384999999999998 | 0.19013484848484907 | 0.026267945547169567 | 0.5682826599326617 | 0 | 0 | 4.391133111168463 | CA3 | F1 | KT2440 | CA3 | F1 | KT2440 | ||||||||||||||
| 0.8358585858585834 | 0.8409090909090935 | 0.7878787878787907 | 0.18288585858585804 | 0.18937272727272786 | 0.20870909090909165 | ||||||||||||||||||||||||
| 0.906565656565652 | 0.8611111111111115 | 0.6414141414141437 | 0.18240101010100918 | 0.18694722222222232 | |||||||||||||||||||||||||
| 0.8863636363636397 | 0.835858585858589 | 0.845959595959598 | 0.2073204545454553 | 0.17477803030303096 | 0.1715606060606065 | ||||||||||||||||||||||||
| P value | 0.148 | P value | 0.838 | ||||||||||||||||||||||||||
| Null hypothesis - TRUE | Null hypothesis - TRUE | ||||||||||||||||||||||||||||
| PHA - Monomer Composition | |||||||||||||||||||||||||||||
| C4 (%) | C5 (%) | C6 (%) | C7 (%) | C8 (%) | C9 (%) | C10 (%) | C12 (%) | Total PHA [as % CDW] | |||||||||||||||||||||
| P. putida CA3 | T24 - 1 | 0 | 0 | 3.4136572771548206 | 0 | 26.783808707582818 | 0 | 69.80253401526237 | 0 | 21.878952684863602 | |||||||||||||||||||
| T24 - 2 | 0 | 0 | 3.4481904942010506 | 0 | 27.691175420901764 | 0 | 68.86063408489719 | 0 | 20.120432072851425 | ||||||||||||||||||||
| T24 - 3 | 0 | 0 | 3.188797425224766 | 0 | 25.869837094068345 | 0 | 70.94136548070689 | 0 | 23.3904393596395 | ||||||||||||||||||||
| Mean | 0 | 0 | 3.350215065526879 | 0 | 26.781607074184308 | 0 | 69.86817786028882 | 0 | 21.796608039118173 | ||||||||||||||||||||
| SD | 0 | 0 | 0.14085409727948012 | 0 | 0.9106711594150253 | 0 | 1.0419177612313963 | 0 | 1.6365580938127382 | ||||||||||||||||||||
| C4 (%) | C5 (%) | C6 (%) | C7 (%) | C8 (%) | C9 (%) | C10 (%) | C12 (%) | Total PHA [as % CDW] | |||||||||||||||||||||
| P. putida F1 | T24 - 1 | 0 | 0 | 2.533105926068193 | 0 | 20.439653550814928 | 0 | 77.02724052311687 | 0 | 22.51956158583293 | |||||||||||||||||||
| T24 - 2 | 0 | 0 | 2.107449829652184 | 0 | 20.34002804065357 | 0 | 77.55252212969425 | 0 | 21.70800656040747 | ||||||||||||||||||||
| T24 - 3 | 0 | 0 | 2.4429688727063885 | 0 | 20.365923532881393 | 0 | 77.19110759441222 | 0 | 20.91173838329656 | ||||||||||||||||||||
| Mean | 0 | 0 | 2.3611748761422553 | 0 | 20.3818683747833 | 0 | 77.25695674907445 | 0 | 21.71310217651232 | ||||||||||||||||||||
| SD | 0 | 0 | 0.2243066461553887 | 0 | 0.05169128607594491 | 0 | 0.2687606275655462 | 0 | 0.8039237131912411 | ||||||||||||||||||||
| C4 (%) | C5 (%) | C6 (%) | C7 (%) | C8 (%) | C9 (%) | C10 (%) | C12 (%) | Total PHA [as % CDW] | |||||||||||||||||||||
| P. putida KT2440 | T24 - 1 | 0 | 0 | 3.5045606254423904 | 0 | 20.490035192951876 | 0 | 76.00540418160571 | 0 | 20.278203110841954 | |||||||||||||||||||
| T24 - 2 | 0 | 0 | 2.8546205023613873 | 0 | 19.95647815044652 | 0 | 77.18890134719209 | 0 | 26.48708076517783 | ||||||||||||||||||||
| Mean | 0 | 0 | 3.179590563901889 | 0 | 20.223256671699197 | 0 | 76.5971527643989 | 0 | 23.38264193800989 | ||||||||||||||||||||
| SD | 0 | 0 | 0.45957706839579887 | 0 | 0.3772818029053748 | 0 | 0.8368588713011834 | 0 | 4.390339492938545 | ||||||||||||||||||||
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| Experiment | Triplicate endpoint flask fermentation | |||||||||||||||||||||||||||||||
| Date | 23/11/2020 | |||||||||||||||||||||||||||||||
| Retention times [min] | ||||||||||||||||||||||||||||||||
| Internal standard | 10.167 | |||||||||||||||||||||||||||||||
| C6 | 10.461 | |||||||||||||||||||||||||||||||
| C8 | 17.103 | |||||||||||||||||||||||||||||||
| C10 | 24.137 | |||||||||||||||||||||||||||||||
| GC - peak area data | Mass of PHA monomer | PHA monomer distribution | ||||||||||||||||||||||||||||||
| Sample name | Sample amount [mg] | Methanolysis conversion efficiency, X | Internal standard peak | C4 | C5 | C6 | C7 | C8 | C9 | C10 | C11 | C12 | C4 | C5 | C6 | C7 | C8 | C9 | C10 | C11 | C12 | Total PHA [mg] | C4 | C5 | C6 | C7 | C8 | C9 | C10 | C11 | C12 | Total %PHA |
| F1-1 | 9.5 | 0.85 | 212.9 | 0 | 0 | 45.9 | 0 | 405.6 | 0 | 1617.6 | 0 | 0 | 0 | 0 | 0.046063381186216314 | 0 | 0.37168581982151244 | 0 | 1.4007053970482803 | 0 | 0 | 1.8184545980560092 | 0 | 0 | 2.533105926068193 | 0 | 20.439653550814928 | 0 | 77.02724052311687 | 0 | 0 | 22.51956158583293 |
| F1-2 | 8.8 | 0.85 | 197.3 | 0 | 0 | 31.6 | 0 | 334 | 0 | 1347.7 | 0 | 0 | 0 | 0 | 0.03421990397640878 | 0 | 0.330273013684744 | 0 | 1.2592659730573263 | 0 | 0 | 1.623758890718479 | 0 | 0 | 2.107449829652184 | 0 | 20.34002804065357 | 0 | 77.55252212969425 | 0 | 0 | 21.70800656040747 |
| F1-3 | 10.9 | 0.85 | 209 | 0 | 0 | 46.3 | 0 | 422.7 | 0 | 1695.5 | 0 | 0 | 0 | 0 | 0.0473318515876468 | 0 | 0.3945841802870813 | 0 | 1.495556529337698 | 0 | 0 | 1.9374725612124262 | 0 | 0 | 2.4429688727063885 | 0 | 20.365923532881393 | 0 | 77.19110759441222 | 0 | 0 | 20.91173838329656 |
| KT-1 | 5.8 | 0.85 | 196.6 | 0 | 0 | 34.3 | 0 | 262.6 | 0 | 1074.9 | 0 | 0 | 0 | 0 | 0.03727600795338944 | 0 | 0.26059430233977615 | 0 | 1.0079427714301012 | 0 | 0 | 1.305813081723267 | 0 | 0 | 2.8546205023613873 | 0 | 19.95647815044652 | 0 | 77.18890134719209 | 0 | 0 | 26.48708076517783 |
| KT-3 | 8 | 0.85 | 216.2 | 0 | 0 | 48.9 | 0 | 313.1 | 0 | 1229.1 | 0 | 0 | 0 | 0 | 0.048325010680346475 | 0 | 0.28254074486586495 | 0 | 1.0480520559910413 | 0 | 0 | 1.378917811537253 | 0 | 0 | 3.5045606254423904 | 0 | 20.490035192951876 | 0 | 76.00540418160571 | 0 | 0 | 20.278203110841954 |
| CA3-1 | 9.1 | 0.85 | 206 | 0 | 0 | 55.7 | 0 | 478.6 | 0 | 1320 | 0 | 0 | 0 | 0 | 0.05777058481906443 | 0 | 0.45327230213592223 | 0 | 1.181294103219213 | 0 | 0 | 1.6923369901741996 | 0 | 0 | 3.4136572771548206 | 0 | 26.783808707582818 | 0 | 69.80253401526237 | 0 | 0 | 21.878952684863602 |
| CA3-2 | 7.5 | 0.85 | 214 | 0 | 0 | 44.3 | 0 | 389.6 | 0 | 1025.3 | 0 | 0 | 0 | 0 | 0.04422916516567544 | 0 | 0.3551884889719626 | 0 | 0.8832598905066402 | 0 | 0 | 1.2826775446442782 | 0 | 0 | 3.4481904942010506 | 0 | 27.691175420901764 | 0 | 68.86063408489719 | 0 | 0 | 20.120432072851425 |
| CA3-3 | 10 | 0.85 | 186.7 | 0 | 0 | 55.4 | 0 | 492.2 | 0 | 1428.4 | 0 | 0 | 0 | 0 | 0.0633992668841603 | 0 | 0.5143408274236744 | 0 | 1.410447251261523 | 0 | 0 | 1.9881873455693575 | 0 | 0 | 3.188797425224766 | 0 | 25.869837094068345 | 0 | 70.94136548070689 | 0 | 0 | 23.3904393596395 |
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| Experiment | Triplicate endpoint flask fermentation | ||||||||
| Date | 2020-12-01T0:0:0+0000 | ||||||||
| Molar Concentration [mM] | BA-Na conc [g/L] | Peak area | RT=5.064 min | ||||||
| 10 | 1.441 | 7426.4 | y=5184.8 | ||||||
| 5 | 0.7205 | 3787.7 | |||||||
| 2.5 | 0.36025 | 1919.1 | |||||||
| 1.25 | 0.180125 | 968.4 | |||||||
| 0.625 | 0.0900625 | 487.5 | |||||||
| 0.3125 | 0.04503125 | 243.3 | |||||||
| 0.15625 | 0.022515625 | 125.2 | |||||||
| 0.078125 | 0.0112578125 | 62.4 | |||||||
| 0.0390625 | 0.00562890625 | 36.9 | |||||||
| Sample | Peak area | BA-Na Concentration | Dilution factor | BA-Na [g/L] | |||||
| T-0 [Control] | 8868.6 | 1.7104999228514117 | 2 | 3.4209998457028234 | |||||
| F1-1 | 0 | 0 | 1 | 0 | |||||
| F1-2 | 0 | 0 | 1 | 0 | |||||
| F1-3 | 0 | 0 | 1 | 0 | |||||
| KT-1 | 0 | 0 | 1 | 0 | |||||
| KT-3 | 0 | 0 | 1 | 0 | |||||
| CA3-1 | 0 | 0 | 1 | 0 | |||||
| CA3-2 | 0 | 0 | 1 | 0 | |||||
| CA3-3 | 0 | 0 | 1 | 0 |
