Selected Cell
Cell:
Value:
Necropsy panel
PBMC LN panels
BAL panels
ALL panels
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| Clone | Marker | Reporter | Channel | Vendor | Catalog # | Lot #s | |||
| 5H9 | CD45RA | BUV395 | UV2 | BDBiosciences | 740315 | ||||
| ICRF44 | CD11b | BUV563 | UV9 | BD Biosciences | 741357 | ||||
| 2ST8.5H7 | CD8b | BUV737 | UV14 | BD Biosciences | 748324 | ||||
| 3.9 | CD11c | BV421 | V1 | BD Biosciences | 565806 | ||||
| GB11 | GranzymeB | V450 | V3 | BD Biosciences | 561151 | ||||
| D058-1283 | CD45 | BV480 | V4 | BD Biosciences | 566145 (bulk order (624248) | ||||
| BL168 | IL-17 | BV510 | V7 | Biolegend | 512330 | ||||
| 3G8 | CD16 | BV570 | V8 | Biolegend | 302036 | ||||
| CD28.2 | CD28 | BV605 | V10 | Biolegend | 302968 | ||||
| Mab11 | TNF | BV650 | V11 | BD Biosciences | 563418 | ||||
| Ber-ACT8 | CD103 | BV711 | V13 | Biolegend | 350222 | ||||
| L200 | CD4 | BV750 | V14 | BD Biosciences | 747202 | ||||
| MQ1-17H12 | IL-2 | BV785 | V15 | Biolegend | 500348 | ||||
| DK25 | CD8a | FITC | B2 | Agilent Technologies | F076501-1 | ||||
| HM47 | CD79a | PerCP-Cy5.5 | B9 | ThermoFisher Scientific | 45-0792-42 | ||||
| 3D12 | CCR7 | PerCP-eFluor710 | B10 | ThermoFisher Scientific | 46-1979-42 | ||||
| 5A6.e9 | Pan gd | PE | YG1 | ThermoFisher Scientific | MHGD04 | ||||
| TP1.55.3 | CD69 | ECD | YG3 | Beckman Coulter | 6607110 | ||||
| H4A3 | CD107a | PE-Cy5 | YG5 | BD | 555802 | ||||
| Z199 | CD159a | PE-Cy7 | YG9 | Beckman Coulter | B10246 | ||||
| B27 | IFNg | APC | R1 | Biolegend | 506510 | ||||
| 2H7 | CD20 | AF647 | R2 | Biolegend | 302318 | ||||
| SP34-2 | CD3 | APC-Cy7 | R7 | BD Biosciences | 557757 | ||||
| - | L/D | ZombieNIR | R8 | Biolegend | 423106 | ||||
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| Panel 1 Feb. 20, 2020-April 28, 2020 | Panel 2: May 6, 2020-August 11, 2020 | Panel 3: Aug. 13, 2020-Feb. 11, 2021 | |||||||||||||||||
| Clone | Marker | Fluor | Aurora Channel | Clone | Marker | Fluor | Aurora Channel | Vendor | Catalog # | Clone | Marker | Fluor | Aurora Channel | Vendor | Catalog # | ||||
| GHI/61 | CD163 | BUV395 | UV2 | 5H9 | CD45RA | BUV395 | UV2 | BDBiosciences | 740315 | 5H9 | CD45RA | BUV395 | UV2 | BDBiosciences | 740315 | ||||
| ICRF44 | CD11b | BUV563 | UV9 | ICRF44 | CD11b | BUV563 | UV9 | BD Biosciences | 741357 | ICRF44 | CD11b | BUV563 | UV9 | BD Biosciences | 741357 | ||||
| 3.9 | CD11c | BV421 | V1 | 3.9 | CD11c | BV421 | V1 | BD Biosciences | 565806 | 3.9 | CD11c | BV421 | V1 | BD Biosciences | |||||
| GB11 | GranzymeB | V450 | V3 | GB11 | GranzymeB | V450 | V3 | BD Biosciences | 561151 | GB11 | GranzymeB | V450 | V3 | BD Biosciences | 561151 | ||||
| 3G8 | CD16 | BV570 | V8 | 3G8 | CD16 | BV570 | V8 | Biolegend | 302036 | 3G8 | CD16 | BV570 | V8 | Biolegend | 302036 | ||||
| CD28.2 | CD28 | BV605 | V10 | CD28.2 | CD28 | BV605 | V10 | Biolegend | 302968 | CD28.2 | CD28 | BV605 | V10 | Biolegend | 302968 | ||||
| 6b11 | iNKT | BV711 | V13 | 6b11 | iNKT | BV711 | V13 | Biolegend | 342922 | Ber-ACT8 | CD103 | BV711 | V13 | Biolegend | 350222 | ||||
| L200 | CD4 | BV750 | V14 | L200 | CD4 | BV750 | V14 | BD Biosciences | 747202 | L200 | CD4 | BV750 | V14 | BD Biosciences | 747202 | ||||
| DK25 | CD8a | FITC | B2 | DK25 | CD8a | FITC | B2 | Agilent Technologies | F076501-1 | DK25 | CD8a | FITC | B2 | Agilent Technologies | F076501-1 | ||||
| HM47 | CD79a | PerCP-Cy5.5 | B9 | HM47 | CD79a | PerCP-Cy5.5 | B9 | ThermoFisher Scientific | 45-0792-42 | HM47 | CD79a | PerCP-Cy5.5 | B9 | ThermoFisher Scientific | 45-0792-42 | ||||
| 3D12 | CCR7 | PerCP-eFluor710 | B10 | 3D12 | CCR7 | PerCP-eFluor710 | B10 | ThermoFisher Scientific | 46-1979-42 | 3D12 | CCR7 | PerCP-eFluor710 | B10 | ThermoFisher Scientific | 46-1979-42 | ||||
| 5A6.e9 | Pan gd | PE | YG1 | 5A6.e9 | Pan gd | PE | YG1 | ThermoFisher Scientific | MHGD04 | 5A6.e9 | Pan gd | PE | YG1 | ThermoFisher Scientific | MHGD04 | ||||
| TP1.55.3 | CD69 | ECD | YG3 | TP1.55.3 | CD69 | ECD | YG3 | Beckman Coulter | 6607110 | TP1.55.3 | CD69 | ECD | YG3 | Beckman Coulter | 6607110 | ||||
| 19.2 | CD206 | PE-Cy5 | YG5 | 19.2 | CD206 | PE-Cy5 | YG5 | BD Biosciences | 551136 | 19.2 | CD206 | PE-Cy5 | YG5 | BD Biosciences | 551136 | ||||
| Z199 | CD159a | PE-Cy7 | YG9 | Z199 | CD159a | PE-Cy7 | YG9 | Beckman Coulter | B10246 | Z199 | CD159a | PE-Cy7 | YG9 | Beckman Coulter | B10246 | ||||
| 2H7 | CD20 | AF647 | R2 | 2H7 | CD20 | AF647 | R2 | Biolegend | 302318 | 2H7 | CD20 | AF647 | R2 | Biolegend | 302318 | ||||
| LN3 | HLA-DR | AF700 | R4 | 27E10 | Calprotectin | AF700 (2 step) | R4 | ThermoFisher Scientific | MA1-40314 | CD107a | AF700 | R4 | |||||||
| SP34-2 | CD3 | APC-Cy7 | R7 | SP34-2 | CD3 | APC-Cy7 | R7 | BD Biosciences | SP34-2 | CD3 | APC-Cy7 | R7 | BD Biosciences | ||||||
| - | Live Dead | ZombieNIR | R8 | - | L/D | ZombieNIR | R8 | Biolegend | 423106 | - | L/D | ZombieNIR | R8 | Biolegend | 423106 | ||||
| Clone | Marker | Fluor | Aurora Channel | Vendor | Catalog # | ||||||||||||||
| 5H9 | CD45RA | BUV395 | UV2 | BDBiosciences | 740315 | ||||||||||||||
| GHI/61 | CD163 | BUV395 | UV2 | BD Biosciences | 745572 | cohort 1 week -4 - 4 | |||||||||||||
| ICRF44 | CD11b | BUV563 | UV9 | BD Biosciences | 741357 | ||||||||||||||
| 3.9 | CD11c | BV421 | V1 | BD Biosciences | 565806 | ||||||||||||||
| GB11 | GranzymeB | V450 | V3 | BD Biosciences | 561151 | ||||||||||||||
| 3G8 | CD16 | BV570 | V8 | Biolegend | 302036 | ||||||||||||||
| CD28.2 | CD28 | BV605 | V10 | Biolegend | 302968 | ||||||||||||||
| 6b11 | CD103 | BV711 | V13 | Biolegend | 342922 | cohort 1 week 7-week 20 | |||||||||||||
| 6b11 | iNKT | BV711 | V13 | Biolegend | 342922 | ||||||||||||||
| L200 | CD4 | BV750 | V14 | BD Biosciences | 747202 | ||||||||||||||
| DK25 | CD8a | FITC | B2 | Agilent Technologies | F076501-1 | ||||||||||||||
| HM47 | CD79a | PerCP-Cy5.5 | B9 | ThermoFisher Scientific | 45-0792-42 | ||||||||||||||
| 3D12 | CCR7 | PerCP-eFluor710 | B10 | ThermoFisher Scientific | 46-1979-42 | ||||||||||||||
| 5A6.e9 | Pan gd | PE | YG1 | ThermoFisher Scientific | MHGD04 | ||||||||||||||
| TP1.55.3 | CD69 | ECD | YG3 | Beckman Coulter | 6607110 | ||||||||||||||
| 19.2 | CD206 | PE-Cy5 | YG5 | BD Biosciences | 551136 | ||||||||||||||
| Z199 | CD159a | PE-Cy7 | YG9 | Beckman Coulter | B10246 | ||||||||||||||
| 2H7 | CD20 | AF647 | R2 | Biolegend | 302318 | ||||||||||||||
| H4A3 | CD107a | AF700 | R4 | BD Biosciences | 561340 | ||||||||||||||
| LN3 | HLA-DR | AF700 | ThermoFisher Scientific | 56-9956-42 | cohort 1 week -4-week 4 | ||||||||||||||
| 2.7E11 | Calprotectin | AF700 (2 step) | R4 | ThermoFisher Scientific | MA1-40314 | cohort 1 week 7-week 20 | |||||||||||||
| SP34-2 | CD3 | APC-Cy7 | R7 | BD Biosciences | |||||||||||||||
| - | L/D | ZombieNIR | R8 | Biolegend | 423106 |
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| Panel 1: Feb. 20, 2020-April 15, 2020 | Panel 3: August 18, 2020-Sept 8, 2020 | Panel 4: Oct 5. 2020-Nov. 23, 2020 | Panel 5: Nov. 30, 2020-Feb. 11-2021 | |||||||||||||||||||||||||
| Clone | Marker | Fluor | Aurora Channel | Vendor | Catalog # | Clone | Marker | Fluor | Aurora Channel | Vendor | Catalog # | Clone | Marker | Fluor | Aurora Channel | Vendor | Catalog # | Clone | Marker | Fluor | Aurora Channel | Vendor | Catalog # | |||||
| GHI/61 | CD163 | BUV395 | UV2 | BD Biosciences | 745572 | cohort 1 week -4 - 4 | 5H9 | CD45RA | BUV395 | UV2 | BDBiosciences | 740315 | 5H9 | CD45RA | BUV395 | UV2 | BDBiosciences | 740315 | 5H9 | CD45RA | BUV395 | UV2 | BDBiosciences | 740315 | ||||
| 5H9 | CD45RA | BUV395 | UV2 | BDBiosciences | 740315 | |||||||||||||||||||||||
| ICRF44 | CD11b | BUV563 | UV9 | BD Biosciences | 741357 | ICRF44 | CD11b | BUV563 | UV9 | BD Biosciences | 741357 | ICRF44 | CD11b | BUV563 | UV9 | BD Biosciences | 741357 | ICRF44 | CD11b | BUV563 | UV9 | BD Biosciences | 741357 | |||||
| 2ST8.5H7 | CD8b | BUV737 | UV14 | BD Biosciences | 748324 | cohort 2 week 12-22 | ||||||||||||||||||||||
| 3.9 | CD11c | BV421 | V1 | BD Biosciences | 741357 | 3.9 | CD11c | BV421 | V1 | BD Biosciences | 741357 | 3.9 | CD11c | BV421 | V1 | BD Biosciences | 741357 | 2ST8.5H7 | CD8b | BUV737 | UV14 | BD Biosciences | 748324 | |||||
| GB11 | GranzymeB | V450 | V3 | BD Biosciences | 561151 | GB11 | Granzyme B | V450 | V3 | BD Biosciences | 561151 | GB11 | Granzyme B | V450 | V3 | BD Biosciences | 561151 | 3.9 | CD11c | BV421 | V1 | BD Biosciences | 741357 | |||||
| D058-1283 | CD45 | BV480 | V4 | BD Biosciences | 566145 (bulk order (624248) | D058-1283 | CD45 | BV480 | V4 | BD Biosciences | 566145 (bulk order (624248) | D058-1283 | CD45 | BV480 | V4 | BD Biosciences | 566145 (bulk order (624248) | GB11 | GranzymeB | V450 | V3 | BD Biosciences | 561151 | |||||
| BL168 | IL-17 | BV510 | V7 | Biolegend | 512330 | BL168 | IL-17 | BV510 | V7 | Biolegend | 512330 | BL168 | IL-17 | BV510 | V7 | Biolegend | 512330 | D058-1283 | CD45 | BV480 | V4 | BD Biosciences | 566145 (bulk order (624248) | |||||
| 3G8 | CD16 | BV570 | V8 | Biolegend | 302036 | 3G8 | CD16 | BV570 | V8 | Biolegend | 302036 | 3G8 | CD16 | BV570 | V8 | Biolegend | 302036 | BL168 | IL-17 | BV510 | V7 | Biolegend | 512330 | |||||
| CD28.2 | CD28 | BV605 | V10 | Biolegend | 302968 | CD28.2 | CD28 | BV605 | V10 | Biolegend | 302968 | CD28.2 | CD28 | BV605 | V10 | Biolegend | 302968 | 3G8 | CD16 | BV570 | V8 | Biolegend | 302036 | |||||
| Mab11 | TNF | BV650 | V11 | BD Biosciences | 563418 | Mab11 | TNF | BV650 | V11 | BD Biosciences | 563418 | Mab11 | TNF | BV650 | V11 | BD Biosciences | 563418 | CD28.2 | CD28 | BV605 | V10 | Biolegend | 302968 | |||||
| 6b11 | iNKT | BV711 | V13 | Biolegend | 342922 | cohort 1 week -4-20 | BerACT8 | CD103 | BV711 | V13 | Biolegend | 342922 | Mab11 | TNF | BV650 | V11 | BD Biosciences | 563418 | ||||||||||
| BerACT8 | CD103 | BV711 | V13 | Biolegend | 342922 | cohort 1 week 22, cohort 2 pre BCG | ||||||||||||||||||||||
| L200 | CD4 | BV750 | V14 | BD Biosciences | 747202 | L200 | CD4 | BV750 | V14 | BD Biosciences | 747202 | L200 | CD4 | BV750 | V14 | BD Biosciences | 747202 | |||||||||||
| MQ1-17H12 | IL-2 | BV785 | V15 | Biolegend | 500348 | MQ1-17H12 | IL-2 | BV785 | V15 | Biolegend | 500348 | MQ1-17H12 | IL-2 | BV785 | V15 | Biolegend | 500348 | L200 | CD4 | BV750 | V14 | BD Biosciences | 747202 | |||||
| DK25 | CD8a | FITC | B2 | Agilent Technologies | F076501-1 | DK25 | CD8a | FITC | B2 | Agilent Technologies | F076501-1 | DK25 | CD8a | FITC | B2 | Agilent Technologies | F076501-1 | MQ1-17H12 | IL-2 | BV785 | V15 | Biolegend | 500348 | |||||
| HM47 | CD79a | PerCP-Cy5.5 | B9 | ThermoFisher Scientific | 45-0792-42 | HM47 | CD79a | PerCP-Cy5.5 | B9 | ThermoFisher Scientific | 45-0792-42 | HM47 | CD79a | PerCP-Cy5.5 | B9 | ThermoFisher Scientific | 45-0792-42 | DK25 | CD8a | FITC | B2 | Agilent Technologies | F076501-1 | |||||
| 3D12 | CCR7 | PerCP-eFluor710 | B10 | ThermoFisher Scientific | 46-1979-42 | 3D12 | CCR7 | PerCP-eFluor710 | B10 | ThermoFisher Scientific | 46-1979-42 | 3D12 | CCR7 | PerCP-eFluor710 | B10 | ThermoFisher Scientific | 46-1979-42 | HM47 | CD79a | PerCP-Cy5.5 | B9 | ThermoFisher Scientific | 45-0792-42 | |||||
| 5A6.e9 | Pan gd | PE | YG1 | ThermoFisher Scientific | MHGD04 | 5A6.e9 | Pan gd | PE | YG1 | ThermoFisher Scientific | MHGD04 | 5A6.e9 | Pan gd | PE | YG1 | ThermoFisher Scientific | MHGD04 | 3D12 | CCR7 | PerCP-eFluor710 | B10 | ThermoFisher Scientific | 46-1979-42 | |||||
| TP1.55.3 | CD69 | ECD | YG3 | Beckman Coulter | 6607110 | TP1.55.3 | CD69 | ECD | YG3 | Beckman Coulter | 6607110 | TP1.55.3 | CD69 | ECD | YG3 | Beckman Coulter | 6607110 | 5A6.e9 | Pan gd | PE | YG1 | ThermoFisher Scientific | MHGD04 | |||||
| 19.2 | CD206 | PE-Cy5 | YG5 | BD Biosciences | 551136 | 19.2 | CD206 | PE-Cy5 | YG5 | BD Biosciences | 551136 | 19.2 | CD206 | PE-Cy5 | YG5 | BD Biosciences | 551136 | TP1.55.3 | CD69 | ECD | YG3 | Beckman Coulter | 6607110 | |||||
| Z199 | CD159a | PE-Cy7 | YG9 | Beckman Coulter | B10246 | Z199 | CD159a | PE-Cy7 | YG9 | Beckman Coulter | B10246 | Z199 | CD159a | PE-Cy7 | YG9 | Beckman Coulter | B10246 | 19.2 | CD206 | PE-Cy5 | YG5 | BD Biosciences | 551136 | |||||
| B27 | IFNg | APC | R1 | Biolegend | 506510 | B27 | IFNg | APC | R1 | Biolegend | 506510 | B27 | IFNg | APC | R1 | Biolegend | 506510 | Z199 | CD159a | PE-Cy7 | YG9 | Beckman Coulter | B10246 | |||||
| 2H7 | CD20 | AF647 | R2 | Biolegend | 302318 | 2H7 | CD20 | AF647 | R2 | Biolegend | 302318 | 2H7 | CD20 | AF647 | R2 | Biolegend | 302318 | B27 | IFNg | APC | R1 | Biolegend | 506510 | |||||
| LN3 | HLA-DR | AF700 | R4 | ThermoFisher Scientific | 56-9956-42 | cohort 1 week -4 - 4 | H4A3 | CD107a | AF700 | R4 | BD Biosciences | 561340 | 2H7 | CD20 | AF647 | R2 | Biolegend | 302318 | ||||||||||
| 2.7E11 | Calprotectin | AF700 (2 step) | R4 | ThermoFisher Scientific | MA1-40314 | cohort 1 week 7-20 | ||||||||||||||||||||||
| H4A3 | CD107a | AF700 | R4 | BD Biosciences | 561340 | cohort 1 week 22, cohort 2 pre BCG | ||||||||||||||||||||||
| SP34-2 | CD3 | APC-Cy7 | R7 | BD Biosciences | SP34-2 | CD3 | APC-Cy7 | R7 | BD Biosciences | SP34-2 | CD3 | APC-Cy7 | R7 | BD Biosciences | ||||||||||||||
| - | L/D | ZombieNIR | R8 | Biolegend | 423106 | - | L/D | ZombieNIR | R8 | Biolegend | 423106 | - | L/D | ZombieNIR | R8 | Biolegend | 423106 | SP34-2 | CD3 | APC-Cy7 | R7 | BD Biosciences | ||||||
| - | L/D | ZombieNIR | R8 | Biolegend | 423106 | |||||||||||||||||||||||
| Clone | Marker | Fluor | Aurora Channel | Vendor | Catalog # | |||||||||||||||||||||||
| 5H9 | CD45RA | BUV395 | UV2 | BDBiosciences | 740315 | |||||||||||||||||||||||
| GHI/61 | CD163 | BUV395 | UV2 | BD Biosciences | 745572 | cohort 1 week -4 - 4 | ||||||||||||||||||||||
| ICRF44 | CD11b | BUV563 | UV9 | BD Biosciences | 741357 | |||||||||||||||||||||||
| 2ST8.5H7 | CD8b | BUV737 | UV14 | BD Biosciences | 748324 | cohort 2 week 12, 22 | ||||||||||||||||||||||
| 3.9 | CD11c | BV421 | V1 | BD Biosciences | 741357 | |||||||||||||||||||||||
| GB11 | GranzymeB | V450 | V3 | BD Biosciences | 561151 | |||||||||||||||||||||||
| D058-1283 | CD45 | BV480 | V4 | BD Biosciences | 566145 (bulk order (624248) | |||||||||||||||||||||||
| BL168 | IL-17 | BV510 | V7 | Biolegend | 512330 | |||||||||||||||||||||||
| 3G8 | CD16 | BV570 | V8 | Biolegend | 302036 | |||||||||||||||||||||||
| CD28.2 | CD28 | BV605 | V10 | Biolegend | 302968 | |||||||||||||||||||||||
| Mab11 | TNF | BV650 | V11 | BD Biosciences | 563418 | |||||||||||||||||||||||
| 6b11 | iNKT | BV711 | V13 | Biolegend | 342922 | cohort 1 week -4-20 | ||||||||||||||||||||||
| BerACT8 | CD103 | BV711 | V13 | Biolegend | 342922 | cohort 1 week 22, cohort 2 pre BCG | ||||||||||||||||||||||
| L200 | CD4 | BV750 | V14 | BD Biosciences | 747202 | |||||||||||||||||||||||
| MQ1-17H12 | IL-2 | BV785 | V15 | Biolegend | 500348 | |||||||||||||||||||||||
| DK25 | CD8a | FITC | B2 | Agilent Technologies | F076501-1 | |||||||||||||||||||||||
| HM47 | CD79a | PerCP-Cy5.5 | B9 | ThermoFisher Scientific | 45-0792-42 | |||||||||||||||||||||||
| 3D12 | CCR7 | PerCP-eFluor710 | B10 | ThermoFisher Scientific | 46-1979-42 | |||||||||||||||||||||||
| 5A6.e9 | Pan gd | PE | YG1 | ThermoFisher Scientific | MHGD04 | |||||||||||||||||||||||
| TP1.55.3 | CD69 | ECD | YG3 | Beckman Coulter | 6607110 | |||||||||||||||||||||||
| 19.2 | CD206 | PE-Cy5 | YG5 | BD Biosciences | 551136 | |||||||||||||||||||||||
| Z199 | CD159a | PE-Cy7 | YG9 | Beckman Coulter | B10246 | |||||||||||||||||||||||
| B27 | IFNg | APC | R1 | Biolegend | 506510 | |||||||||||||||||||||||
| 2H7 | CD20 | AF647 | R2 | Biolegend | 302318 | |||||||||||||||||||||||
| LN3 | HLA-DR | AF700 | R4 | ThermoFisher Scientific | 56-9956-42 | cohort 1 week -4 - 4 | ||||||||||||||||||||||
| 2.7E11 | Calprotectin | AF700 (2 step) | R4 | ThermoFisher Scientific | MA1-40314 | cohort 1 week 7-20 | ||||||||||||||||||||||
| H4A3 | CD107a | AF700 | R4 | BD Biosciences | 561340 | cohort 1 week 22, cohort 2 pre BCG | ||||||||||||||||||||||
| SP34-2 | CD3 | APC-Cy7 | R7 | BD Biosciences | ||||||||||||||||||||||||
| - | L/D | ZombieNIR | R8 | Biolegend | 423106 |
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| BAL Panels | |||||||||
| Clone | Marker | Fluor | Aurora Channel | Vendor | Catalog # | cohort/time point | |||
| 5H9 | CD45RA | BUV395 | UV2 | BD Biosciences | 740315 | cohort 1 week 7-22, all cohort 2 | |||
| GHI/61 | CD163 | BUV395 | UV2 | BD Biosciences | 745572 | cohort 1 week -4 - 4 only | |||
| ICRF44 | CD11b | BUV563 | UV9 | BD Biosciences | 741357 | cohort 1 and 2 all time points | |||
| 2ST8.5H7 | CD8b | BUV737 | UV14 | BD Biosciences | 748324 | cohort 2 week 12, 22 only | |||
| 3.9 | CD11c | BV421 | V1 | BD Biosciences | 741357 | cohort 1 and 2 all time points | |||
| GB11 | GranzymeB | V450 | V3 | BD Biosciences | 561151 | cohort 1 and 2 all time points | |||
| D058-1283 | CD45 | BV480 | V4 | BD Biosciences | 566145 (bulk order (624248) | cohort 1 and 2 all time points | |||
| BL168 | IL-17 | BV510 | V7 | Biolegend | 512330 | cohort 1 and 2 all time points | |||
| 3G8 | CD16 | BV570 | V8 | Biolegend | 302036 | cohort 1 and 2 all time points | |||
| CD28.2 | CD28 | BV605 | V10 | Biolegend | 302968 | cohort 1 and 2 all time points | |||
| Mab11 | TNF | BV650 | V11 | BD Biosciences | 563418 | cohort 1 and 2 all time points | |||
| 6b11 | iNKT | BV711 | V13 | Biolegend | 342922 | cohort 1 week -4-20 only | |||
| BerACT8 | CD103 | BV711 | V13 | Biolegend | 342922 | cohort 1 week 22, cohort 2 pre BCG | |||
| L200 | CD4 | BV750 | V14 | BD Biosciences | 747202 | cohort 1 and 2 all time points | |||
| MQ1-17H12 | IL-2 | BV785 | V15 | Biolegend | 500348 | cohort 1 and 2 all time points | |||
| DK25 | CD8a | FITC | B2 | Agilent Technologies | F076501-1 | cohort 1 and 2 all time points | |||
| HM47 | CD79a | PerCP-Cy5.5 | B9 | ThermoFisher Scientific | 45-0792-42 | cohort 1 and 2 all time points | |||
| 3D12 | CCR7 | PerCP-eFluor710 | B10 | ThermoFisher Scientific | 46-1979-42 | cohort 1 and 2 all time points | |||
| 5A6.e9 | Pan gd | PE | YG1 | ThermoFisher Scientific | MHGD04 | cohort 1 and 2 all time points | |||
| TP1.55.3 | CD69 | ECD | YG3 | Beckman Coulter | 6607110 | cohort 1 and 2 all time points | |||
| 19.2 | CD206 | PE-Cy5 | YG5 | BD Biosciences | 551136 | cohort 1 and 2 all time points | |||
| Z199 | CD159a | PE-Cy7 | YG9 | Beckman Coulter | B10246 | cohort 1 and 2 all time points | |||
| B27 | IFNg | APC | R1 | Biolegend | 506510 | cohort 1 and 2 all time points | |||
| 2H7 | CD20 | AF647 | R2 | Biolegend | 302318 | cohort 1 and 2 all time points | |||
| LN3 | HLA-DR | AF700 | R4 | ThermoFisher Scientific | 56-9956-42 | cohort 1 week -4 - 4 only | |||
| 2.7E11 | Calprotectin | AF700 (2 step) | R4 | ThermoFisher Scientific | MA1-40314 | cohort 1 week 7-12 only | |||
| H4A3 | CD107a | AF700 | R4 | BD Biosciences | 561340 | cohort 1 week 22, cohort 2 pre BCG | |||
| SP34-2 | CD3 | APC-Cy7 | R7 | BD Biosciences | cohort 1 and 2 all time points | ||||
| - | L/D | ZombieNIR | R8 | Biolegend | 423106 | cohort 1 and 2 all time points | |||
| PBMC and LN Biopsy Panel | |||||||||
| Clone | Marker | Fluor | Aurora Channel | Vendor | Catalog # | ||||
| 5H9 | CD45RA | BUV395 | UV2 | BD Biosciences | 740315 | cohort 1 week 7-22, all cohort 2 | |||
| GHI/61 | CD163 | BUV395 | UV2 | BD Biosciences | 745572 | cohort 1 week -4 - 4 only | |||
| ICRF44 | CD11b | BUV563 | UV9 | BD Biosciences | 741357 | cohort 1 and 2 all time points | |||
| 3.9 | CD11c | BV421 | V1 | BD Biosciences | 741357 | cohort 1 and 2 all time points | |||
| GB11 | GranzymeB | V450 | V3 | BD Biosciences | 561151 | cohort 1 and 2 all time points | |||
| 3G8 | CD16 | BV570 | V8 | Biolegend | 302036 | cohort 1 and 2 all time points | |||
| CD28.2 | CD28 | BV605 | V10 | Biolegend | 302968 | cohort 1 and 2 all time points | |||
| 6b11 | CD103 | BV711 | V13 | Biolegend | 342922 | cohort 1 week 7-week 20 | |||
| 6b11 | iNKT | BV711 | V13 | Biolegend | 342922 | cohort 1 week -4-20 only | |||
| L200 | CD4 | BV750 | V14 | BD Biosciences | 747202 | cohort 1 and 2 all time points | |||
| DK25 | CD8a | FITC | B2 | Agilent Technologies | F076501-1 | cohort 1 and 2 all time points | |||
| HM47 | CD79a | PerCP-Cy5.5 | B9 | ThermoFisher Scientific | 45-0792-42 | cohort 1 and 2 all time points | |||
| 3D12 | CCR7 | PerCP-eFluor710 | B10 | ThermoFisher Scientific | 46-1979-42 | cohort 1 and 2 all time points | |||
| 5A6.e9 | Pan gd | PE | YG1 | ThermoFisher Scientific | MHGD04 | cohort 1 and 2 all time points | |||
| TP1.55.3 | CD69 | ECD | YG3 | Beckman Coulter | 6607110 | cohort 1 and 2 all time points | |||
| 19.2 | CD206 | PE-Cy5 | YG5 | BD Biosciences | 551136 | cohort 1 and 2 all time points | |||
| Z199 | CD159a | PE-Cy7 | YG9 | Beckman Coulter | B10246 | cohort 1 and 2 all time points | |||
| 2H7 | CD20 | AF647 | R2 | Biolegend | 302318 | cohort 1 and 2 all time points | |||
| H4A3 | CD107a | AF700 | R4 | BD Biosciences | 561340 | cohort 1 week 22, cohort 2 pre BCG | |||
| LN3 | HLA-DR | AF700 | ThermoFisher Scientific | 56-9956-42 | cohort 1 week -4- 4 only | ||||
| 2.7E11 | Calprotectin | AF700 (2 step) | R4 | ThermoFisher Scientific | MA1-40314 | cohort 1 week 7-12 only | |||
| SP34-2 | CD3 | APC-Cy7 | R7 | BD Biosciences | cohort 1 and 2 all time points | ||||
| - | L/D | ZombieNIR | R8 | Biolegend | 423106 | cohort 1 and 2 all time points | |||
| NECROPSY Panel | |||||||||
| Clone | Marker | Reporter | Channel | Vendor | Catalog # | Lot #s | |||
| 5H9 | CD45RA | BUV395 | UV2 | BDBiosciences | 740315 | cohort 1 and 2 all time points | |||
| ICRF44 | CD11b | BUV563 | UV9 | BD Biosciences | 741357 | cohort 1 and 2 all time points | |||
| 2ST8.5H7 | CD8b | BUV737 | UV14 | BD Biosciences | 748324 | cohort 1 and 2 all time points | |||
| 3.9 | CD11c | BV421 | V1 | BD Biosciences | 741357 | cohort 1 and 2 all time points | |||
| GB11 | GranzymeB | V450 | V3 | BD Biosciences | 561151 | cohort 1 and 2 all time points | |||
| D058-1283 | CD45 | BV480 | V4 | BD Biosciences | 566145 (bulk order (624248) | cohort 1 and 2 all time points | |||
| BL168 | IL-17 | BV510 | V7 | Biolegend | 512330 | cohort 1 and 2 all time points | |||
| 3G8 | CD16 | BV570 | V8 | Biolegend | 302036 | cohort 1 and 2 all time points | |||
| CD28.2 | CD28 | BV605 | V10 | Biolegend | 302968 | cohort 1 and 2 all time points | |||
| Mab11 | TNF | BV650 | V11 | BD Biosciences | 563418 | cohort 1 and 2 all time points | |||
| 6b11 | CD103 | BV711 | V13 | Biolegend | 350222 | cohort 1 and 2 all time points | |||
| L200 | CD4 | BV750 | V14 | BD Biosciences | 747202 | cohort 1 and 2 all time points | |||
| MQ1-17H12 | IL-2 | BV785 | V15 | Biolegend | 500348 | cohort 1 and 2 all time points | |||
| DK25 | CD8a | FITC | B2 | Agilent Technologies | F076501-1 | cohort 1 and 2 all time points | |||
| HM47 | CD79a | PerCP-Cy5.5 | B9 | ThermoFisher Scientific | 45-0792-42 | cohort 1 and 2 all time points | |||
| 3D12 | CCR7 | PerCP-eFluor710 | B10 | ThermoFisher Scientific | 46-1979-42 | cohort 1 and 2 all time points | |||
| 5A6.e9 | Pan gd | PE | YG1 | ThermoFisher Scientific | MHGD04 | cohort 1 and 2 all time points | |||
| TP1.55.3 | CD69 | ECD | YG3 | Beckman Coulter | 6607110 | cohort 1 and 2 all time points | |||
| H4A3 | CD107a | PE-Cy5 | YG5 | BD | 555802 | cohort 1 and 2 all time points | |||
| Z199 | CD159a | PE-Cy7 | YG9 | Beckman Coulter | B10246 | cohort 1 and 2 all time points | |||
| B27 | IFNg | APC | R1 | Biolegend | 506510 | cohort 1 and 2 all time points | |||
| 2H7 | CD20 | AF647 | R2 | Biolegend | 302318 | cohort 1 and 2 all time points | |||
| SP34-2 | CD3 | APC-Cy7 | R7 | BD Biosciences | cohort 1 and 2 all time points | ||||
| - | L/D | ZombieNIR | R8 | Biolegend | 423106 | cohort 1 and 2 all time points | |||
