Regulation of Rubisco activity by interaction with chloroplast metabolites

Selected Cell

Cell:

Value:

Dictionary Figure_1_2_S1 Figure_3 Figure_4 Figure_S2 Figure_S3 Figure_S4 Figure_S5 Table S3

Reference: Lobo AKM, Orr DJ, Carmo-Silva E. (2024) Regulation of Rubisco activity by interaction with chloroplast metabolites. Biochemical Journal, https://doi.org/10.1042/BCJ20240209.

Abbreciations	Description
Met.conc	Mtabolite concentration
Sample	Metabolites used in the study. See Table S1.
Unit	Metabolite concentration unit
Relative_Vel	Rubisco velocity (activity) relative to the control (0 metabolite concentration)
Abs_Vel	Rubisco velocity (activity) absolute values
Day	Day of analysis
S_uM	Concentration of substrate in µM
Vmax-Rel	Rubisco maximum velocity relative to the control (0 metabolite concentration)
Km_Rel	Michaelis-Menten constant relative to the control (0 metabolite concentration)
Ki app	Apparent inhibitor constant

100

Number	Sample	Met.conc	Unit	Relative_Vel	Abs_Vel	Day
1	2-PG	0	uM	106.3402235	1.7029561530840303	1
2	2-PG	0	uM	107.951657	1.7287620100919332	1
3	2-PG	0	uM	85.70811951	1.372549019607843	1
4	2-PG	1.5	uM	106.8869599	1.7117117117117118	1
5	2-PG	1.5	uM	107.6351254	1.723693002465381	1
6	2-PG	1.5	uM	95.34794494	1.5269233427801203	1
7	2-PG	3	uM	104.9302192	1.680376028202115	1
8	2-PG	3	uM	105.6783847	1.6923573189557843	1
9	2-PG	3	uM	94.25447221	1.5094122255247573	1
10	2-PG	9	uM	106.4553259	1.7047994285845947	1
11	2-PG	9	uM	105.6496091	1.6918965000806432	1
12	2-PG	9	uM	95.57814973	1.5306098937812491	1
13	2-PG	18	uM	108.6998226	1.7407433008456026	1
14	2-PG	18	uM	103.0598053	1.650422801317942	1
15	2-PG	18	uM	96.0385593	1.5379829957835074	1
16	2-PG	28	uM	110.1098269	1.7633234257275177	1
17	2-PG	28	uM	106.3977747	1.7038777908343123	1
18	2-PG	28	uM	95.80835452	1.5342964447823784	1
19	3-PGA	0	mM	100.13661548832316	1.15	1
20	3-PGA	0	mM	103.3336620850487	1.18671587628866	1
21	3-PGA	0	mM	96.52972242662814	1.1085773195876292	1
22	3-PGA	1	mM	89.82032281035414	1.03152449009975	1
23	3-PGA	1	mM	83.08193510740284	0.9541387524192346	1
24	3-PGA	1	mM	97.33569874980194	1.117833402061856	1
25	3-PGA	1	mM	97.93549508332657	1.124721649484536	1
26	3-PGA	2	mM	86.51076070846202	0.9935164507964866	1
27	3-PGA	2	mM	85.64610033949924	0.9835864225100489	1
28	3-PGA	2	mM	102.24653123053524	1.1742309278350518	1
29	3-PGA	2	mM	94.0743061862615	1.0803785567010313	1
30	3-PGA	4	mM	85.46720509074834	0.9815319338990619	1
31	3-PGA	4	mM	79.11642376009095	0.9085975882090218	1
32	3-PGA	4	mM	99.8098586255912	1.1462474226804125	1
33	3-PGA	4	mM	89.38839733059994	1.0265641237113408	1
34	3-PGA	8	mM	76.07520453132543	0.8736712818222417	1
35	3-PGA	8	mM	75.18072828757089	0.8633988387673067	1
36	3-PGA	8	mM	84.36510303733074	0.9688750515463921	1
37	3-PGA	8	mM	83.99023032887781	0.9645698969072168	1
38	3-PGA	12	mM	66.77265159627801	0.7668378740509155	1
39	3-PGA	12	mM	56.3967271687251	0.6476775346136668	1
40	3-PGA	12	mM	79.15437238983507	0.9090334020618559	1
41	3-PGA	12	mM	71.1695836997878	0.8173336082474232	1
42	CA1P	0	uM	101.9800715	0.8592012371134022	1
43	CA1P	0	uM	98.01992846	0.8258362886597939	1
44	CA1P	0	uM	100.1471791	1.3182383505154642	2
45	CA1P	0	uM	99.85282093	1.314363711340207	2
46	CA1P	0.15	uM	75.71538068	0.6379162886597939	1
47	CA1P	0.15	uM	68.61267246	0.5780746391752578	1
48	CA1P	0.15	uM	71.82338512	0.9454119587628868	2
49	CA1P	0.15	uM	67.44071954	0.8877228865979384	2
50	CA1P	0.15	uM	69.69746525	0.9174284536082475	2
52	CA1P	0.3	uM	57.95861012	0.48831216494845375	1
53	CA1P	0.3	uM	56.15699101	0.739195051546392	2
54	CA1P	0.3	uM	52.65739984	0.6931298969072167	2
55	CA1P	0.3	uM	54.16189697	0.7129336082474228	2
57	CA1P	0.6	uM	44.28972918	0.3731492783505155	1
58	CA1P	0.6	uM	45.00408831	0.5923892783505156	2
60	CA1P	0.6	uM	43.76124285	0.5760296907216496	2
62	CA1P	1	uM	34.91313235	0.29414969072164954	1
63	CA1P	1	uM	35.25756337	0.4640956701030928	2
64	CA1P	1	uM	34.89779231	0.45936000000000016	2
65	CA1P	1	uM	34.53802126	0.45462432989690743	2
66	CA1P	1.5	uM	32.66479305	0.27520701030927847	1
67	CA1P	1.5	uM	29.36893204	0.24743876288659797	1
68	CA1P	1.5	uM	29.69746525	0.39090804123711365	2
69	CA1P	1.5	uM	27.60425184	0.36335505154639186	2
70	CA1P	1.5	uM	27.01553557	0.35560577319587644	2
71	CABP	0	uM	103.504168	1.4433030927835053	1
72	CABP	0	uM	96.49583205	1.345576082474227	1
73	CABP	0	uM	96.59570477	1.1085773195876292	2
74	CABP	0	uM	103.4042952	1.18671587628866	2
75	CABP	0.05	uM	86.04507564	1.1998465979381445	1
76	CABP	0.05	uM	76.3661624	1.0648800000000003	1
77	CABP	0.05	uM	78.77707962	0.9040824742268043	2
78	CABP	0.05	uM	71.27450061	0.8179793814432991	2
79	CABP	0.1	uM	67.58258722	0.9423983505154641	1
80	CABP	0.1	uM	58.76813831	0.8194861855670105	1
81	CABP	0.1	uM	61.89627685	0.7103505154639177	2
82	CABP	0.1	uM	59.75804183	0.6858111340206189	2
83	CABP	0.15	uM	47.79252856	0.6664379381443299	1
84	CABP	0.15	uM	51.94504477	0.7243422680412372	1
85	CABP	0.15	uM	43.81506143	0.5028420618556704	2
86	CABP	0.15	uM	37.70045953	0.43266804123711355	2
88	CABP	0.2	uM	31.16702686	0.4346053608247423	1
89	CABP	0.2	uM	31.09819	0.35689731958762905	2
90	CABP	0.2	uM	30.31041921	0.347856494845361	2
91	CABP	0.3	uM	15.18987342	0.21181360824742274	1
92	CABP	0.3	uM	14.83482556	0.20686268041237119	1
93	CABP	0.3	uM	14.06733565	0.16144329896907225	2
94	CABP	0.3	uM	13.80474538	0.15842969072164956	2
95	DHAP	0	mM	100.30700076530626	1.0126	1
96	DHAP	0	mM	99.69299923469373	1.0064016494845365	1
97	DHAP	0.2	mM	88.11532640914628	0.8895249468246734	1
98	DHAP	0.2	mM	86.77424222996865	0.8759866909753873	1
99	DHAP	0.2	mM	106.33872533511	1.0734903092783508	1
100	DHAP	0.2	mM	100.5814652908991	1.015370721649485	1
101	DHAP	0.4	mM	90.18791104969345	0.9104477058644787	1
102	DHAP	0.4	mM	83.45201096791507	0.8424487389851109	1
103	DHAP	0.4	mM	99.68589150624409	1.006329896907217	1
104	DHAP	0.4	mM	94.52568065180323	0.9542375257731964	1

Number	Met	Met.conc	Unit	Vmax_Rel	Abs_Vel_Vmax	Km_Rel	Abs_Vel_Km
1	CABP	0	uM	82.91814947	2.33	89.27432217	44.78
2	CABP	0	uM	108.5409253	3.05	126.2360447	63.32
3	CABP	0	uM	108.5409253	3.05	84.48963317	42.38
4	CABP	0.15	uM	49.46619217	1.39	131.0207337	65.72
5	CABP	0.15	uM	61.5658363	1.73	92.64354067	46.47
6	CABP	0.15	uM	61.5658363	1.73	79.10685805	39.68
7	CABP	0.2	uM	38.07829181	1.07	123.0661882	61.73
8	CABP	0.2	uM	43.06049822	1.21	91.20813397	45.75
9	CABP	0.2	uM	64.0569395	1.8	145.5940989	73.03
10	CA1P	0	uM	95.21640091	2.09	92.50709789	37.47
11	CA1P	0	uM	104.7835991	2.3	107.4929021	43.54
12	CA1P	0.6	uM	28.70159453	0.63	45.35242563	18.37
13	CA1P	0.6	uM	30.06833713	0.66	46.01901	18.64
14	CA1P	0.6	uM	29.61275626	0.65	47.37686705	19.19
15	CA1P	1.5	uM	15.03416856	0.33	28.61375139	11.59
16	CA1P	1.5	uM	11.38952164	0.25	14.56610295	5.9
17	CA1P	1.5	uM	14.5785877	0.32	21.40476484	8.67
18	3-PGA	0	mM	102.1518987	2.69	99.68481601	42.17
19	3-PGA	0	mM	97.97468354	2.58	106.4455126	45.03
20	3-PGA	0	mM	99.87341772	2.63	93.86967142	39.71
21	3-PGA	8	mM	74.81012658	1.97	81.12835868	34.32
22	3-PGA	8	mM	74.05063291	1.95	77.96075959	32.98
23	3-PGA	8	mM	85.06329114	2.24	89.35466078	37.8
24	3-PGA	12	mM	59.62025316	1.57	68.81254432	29.11
25	3-PGA	12	mM	56.96202532	1.5	62.85556694	26.59
26	3-PGA	12	mM	64.93670886	1.71	73.28027736	31
27	F6P	0	mM	101.6528926	3.28	95.17309594	48.11
28	F6P	0	mM	105.9917355	3.42	102.0771513	51.6
29	F6P	0	mM	92.3553719	2.98	102.7497527	51.94
30	F6P	2	mM	93.90495868	3.03	87.39861523	44.18
31	F6P	2	mM	95.45454545	3.08	115.2522255	58.26
32	F6P	2	mM	89.5661157	2.89	106.5281899	53.85
33	F6P	6	mM	91.73553719	2.96	104.0158259	52.58
34	F6P	6	mM	75.30991736	2.43	103.5410485	52.34
35	F6P	6	mM	58.88429752	1.9	59.48565776	30.07
36	G6P	0	mM	96.47779479	2.1	98.74275611	33.51
37	G6P	0	mM	96.47779479	2.1	84.18622925	28.57
38	G6P	0	mM	107.0444104	2.33	117.0710146	39.73
39	G6P	2	mM	99.69372129	2.17	108.790885	36.92
40	G6P	2	mM	110.719755	2.41	121.7562126	41.32
41	G6P	2	mM	113.9356815	2.48	141.1158039	47.89
42	G6P	6	mM	113.0168453	2.46	146.0367351	49.56
43	G6P	6	mM	118.0704441	2.57	154.3757981	52.39
44	G6P	6	mM	98.77488515	2.15	68.4805029	23.24

Number	Met	Met.conc	Unit	Vmax_Rel	Abs_Vel_Vmax	Km_Rel	Abs_Vel_Km
1	3-PGA	0	mM	98.12734082	1.31	104.9650073	79.49
2	3-PGA	0	mM	92.13483146	1.23	94.91614948	71.88
3	3-PGA	0	mM	101.1235955	1.35	98.86438664	74.87
4	3-PGA	0	mM	108.6142322	1.45	101.2544566	76.68
5	3-PGA	8	mM	98.12734082	1.31	281.5528853	213.22
6	3-PGA	8	mM	106.3670412	1.42	360.0290506	272.65
7	3-PGA	12	mM	100.3745318	1.34	521.8143404	395.17
8	3-PGA	12	mM	105.6179775	1.41	486.4650733	368.4
9	CA1P	0	uM	98.12734082	1.31	104.9650073	79.49
10	CA1P	0	uM	92.13483146	1.23	94.91614948	71.88
11	CA1P	0	uM	101.1235955	1.35	98.86438664	74.87
12	CA1P	0	uM	108.6142322	1.45	101.2544566	76.68
13	CA1P	0.6	uM	37.45318352	0.5	119.8204146	90.74
14	CA1P	0.6	uM	39.70037453	0.53	157.4673181	119.25
15	CA1P	1.5	uM	25.46816479	0.34	202.8786478	153.64
16	CA1P	1.5	uM	25.46816479	0.34	213.6933844	161.83
17	CABP	0	uM	98.12734082	1.31	104.9650073	79.49
18	CABP	0	uM	92.13483146	1.23	94.91614948	71.88
19	CABP	0	uM	101.1235955	1.35	98.86438664	74.87
20	CABP	0	uM	108.6142322	1.45	101.2544566	76.68
21	CABP	0.15	uM	41.19850187	0.55	62.15502443	47.07
22	CABP	0.15	uM	46.44194757	0.62	105.3611515	79.79
23	CABP	0.2	uM	41.94756554	0.56	155.0772481	117.44
24	CABP	0.2	uM	31.46067416	0.42	89.95114222	68.12
25	F6P	0	mM	99.83844911	2.06	95.65838646	87.03
26	F6P	0	mM	102.2617124	2.11	116.1683887	105.69
27	F6P	0	mM	97.89983845	2.02	88.17322488	80.22
28	F6P	2	mM	91.11470113	1.88	93.67992965	85.23
29	F6P	2	mM	93.05331179	1.92	90.98703012	82.78
30	F6P	2	mM	94.99192246	1.96	98.8898659	89.97
31	F6P	6	mM	87.72213247	1.81	105.2209277	95.73
32	F6P	6	mM	89.17609047	1.84	99.21960871	90.27
33	F6P	6	mM	88.20678514	1.82	103.0556166	93.76
34	G6P	0	mM	99.10714286	1.85	97.10726644	70.16
35	G6P	0	mM	97.5	1.82	91.10034602	65.82
36	G6P	0	mM	103.3928571	1.93	111.7923875	80.77
37	G6P	2	mM	96.42857143	1.8	92.92733564	67.14
38	G6P	2	mM	99.64285714	1.86	112.7750865	81.48
39	G6P	2	mM	98.03571429	1.83	107.4186851	77.61
40	G6P	6	mM	98.03571429	1.83	118.5605536	85.66
41	G6P	6	mM	93.21428571	1.74	104.9273356	75.81
42	G6P	6	mM	92.14285714	1.72	108.8027682	78.61

100

Rep	Sample	Met.conc	Unit	S_uM	Relative_Vel	Abs_Vel
1	3-PGA	0	mM	4	13.14956557	0.241929909
2	3-PGA	0	mM	4	11.98071534	0.220425029
3	3-PGA	0	mM	4	13.20522511	0.242953951
1	3-PGA	0	mM	16	35.33105467	0.650032033
2	3-PGA	0	mM	16	35.48650784	0.65289211
3	3-PGA	0	mM	16	36.54013499	0.672277079
1	3-PGA	0	mM	36	72.37234962	1.331529613
2	3-PGA	0	mM	36	64.61154572	1.188743863
3	3-PGA	0	mM	36	72.57207623	1.335204247
1	3-PGA	0	mM	68	90.28934039	1.661172134
2	3-PGA	0	mM	68	81.45057374	1.498553681
3	3-PGA	0	mM	68	91.62854748	1.685811294
1	3-PGA	0	mM	100	101.5719151	1.868752549
2	3-PGA	0	mM	100	97.90411318	1.801271157
3	3-PGA	0	mM	100	100.5239717	1.849472151
1	3-PGA	8	mM	4	10.67271621	0.196360043
2	3-PGA	8	mM	4	14.20709678	0.261386706
3	3-PGA	8	mM	4	12.11986414	0.222985133
1	3-PGA	8	mM	16	33.03380204	0.607766445
2	3-PGA	8	mM	16	32.7056231	0.601728504
3	3-PGA	8	mM	16	34.0010663	0.625562482
1	3-PGA	8	mM	36	55.85210898	1.027584947
2	3-PGA	8	mM	36	54.88200847	1.009736728
3	3-PGA	8	mM	36	60.8738056	1.119975726
1	3-PGA	8	mM	68	71.27260005	1.311296069
2	3-PGA	8	mM	68	73.68317276	1.355646556
3	3-PGA	8	mM	68	79.25427418	1.45814546
1	3-PGA	8	mM	100	78.88394025	1.451331938
2	3-PGA	8	mM	100	78.30757136	1.440727719
3	3-PGA	8	mM	100	86.95310449	1.599791
1	3-PGA	12	mM	4	10.06046132	0.185095582
2	3-PGA	12	mM	4	10.28309943	0.189191749
3	3-PGA	12	mM	4	10.81186506	0.198920148
1	3-PGA	12	mM	16	27.76566628	0.5108416
2	3-PGA	12	mM	16	28.18020811	0.518468473
3	3-PGA	12	mM	16	29.63110453	0.545162529
1	3-PGA	12	mM	36	49.57498819	0.912096473
2	3-PGA	12	mM	36	49.71765002	0.914721211
3	3-PGA	12	mM	36	51.0586713	0.939393749
1	3-PGA	12	mM	68	60.93392152	1.121081758
2	3-PGA	12	mM	68	60.02326069	1.104327129
3	3-PGA	12	mM	68	65.80863521	1.210768298
1	3-PGA	12	mM	100	64.05554097	1.17851431
2	3-PGA	12	mM	100	62.37883153	1.147665674
3	3-PGA	12	mM	100	68.82368351	1.26624012
1	CA1P	0	uM	4	10.75296152	0.163667458
2	CA1P	0	uM	4	12.70804546	0.193425178
1	CA1P	0	uM	16	38.53747824	0.586566881
2	CA1P	0	uM	16	40.55985323	0.617348817
1	CA1P	0	uM	36	70.63804072	1.075159484
2	CA1P	0	uM	36	65.9610081	1.003971836
1	CA1P	0	uM	68	89.41212781	1.360913981
2	CA1P	0	uM	68	97.49615495	1.483958425
1	CA1P	0	uM	100	97.6823449	1.486792364
2	CA1P	0	uM	100	102.3176551	1.557344968
1	CA1P	0.6	uM	4	5.177351834	0.07880285
2	CA1P	0.6	uM	4	5.249762365	0.079904988
3	CA1P	0.6	uM	4	5.249762365	0.079904988
1	CA1P	0.6	uM	16	18.94291207	0.288324129
2	CA1P	0.6	uM	16	19.12267873	0.291060301
3	CA1P	0.6	uM	16	20.06645369	0.305425204
1	CA1P	0.6	uM	36	29.65832607	0.451420088
2	CA1P	0.6	uM	36	30.92038254	0.470629454
3	CA1P	0.6	uM	36	29.95528055	0.455939939
1	CA1P	0.6	uM	68	34.07835583	0.518695976
2	CA1P	0.6	uM	68	36.16905251	0.550517815
3	CA1P	0.6	uM	68	32.684558	0.497481416
1	CA1P	0.6	uM	100	32.65167007	0.496980839
2	CA1P	0.6	uM	100	33.06066804	0.503206069
3	CA1P	0.6	uM	100	35.92365372	0.546782677
1	CA1P	1.5	uM	4	3.367088959	0.051249406
2	CA1P	1.5	uM	4	3.51191002	0.053453682
3	CA1P	1.5	uM	4	5.322172896	0.081007126
1	CA1P	1.5	uM	16	13.01061216	0.198030451
2	CA1P	1.5	uM	16	13.23532055	0.201450667
3	CA1P	1.5	uM	16	14.22403718	0.216499613
1	CA1P	1.5	uM	36	19.04220437	0.289835426
2	CA1P	1.5	uM	36	19.71035189	0.30000509
3	CA1P	1.5	uM	36	19.93306772	0.303394978
1	CA1P	1.5	uM	68	19.02533975	0.289578735
2	CA1P	1.5	uM	68	17.35278243	0.264121264
3	CA1P	1.5	uM	68	18.60720041	0.283214367
1	CA1P	1.5	uM	100	17.24608041	0.262497186
2	CA1P	1.5	uM	100	8.24812545	0.125542133
3	CA1P	1.5	uM	100	18.20040897	0.277022722
1	CABP	0	uM	4	9.79517048	0.183047498
2	CABP	0	uM	4	10.39795018	0.194311959
3	CABP	0	uM	4	19.00126072	0.355086544
1	CABP	0	uM	16	30.72001638	0.574081089
2	CABP	0	uM	16	31.72332717	0.592830485
3	CABP	0	uM	16	40.46403487	0.756172683
1	CABP	0	uM	36	57.99346424	1.083754341
2	CABP	0	uM	36	63.6397158	1.189268811
3	CABP	0	uM	36	74.48276606	1.391898589
1	CABP	0	uM	68	74.75790976	1.397040344
2	CABP	0	uM	68	77.44761237	1.44730423
3	CABP	0	uM	68	106.2959909	1.98640904
1	CABP	0	uM	100	85.55584217	1.598826981
2	CABP	0	uM	100	103.3015218	1.930449821

100

Rep	Sample	Met.conc	Unit	S_uM	Relative_Vel	Abs_Vel
1	3-PGA	0	mM	10	15.86037965	0.192681235
2	3-PGA	0	mM	10	12.43110842	0.151020428
3	3-PGA	0	mM	10	13.37007549	0.162427553
4	3-PGA	0	mM	10	15.1255358	0.183753919
1	3-PGA	0	mM	50	44.51928966	0.540846556
2	3-PGA	0	mM	50	43.9885691	0.53439905
3	3-PGA	0	mM	50	42.60053071	0.517536342
4	3-PGA	0	mM	50	48.60175543	0.590442755
1	3-PGA	0	mM	150	67.09532555	0.815113539
2	3-PGA	0	mM	150	66.8503776	0.812137767
3	3-PGA	0	mM	150	77.38313941	0.940095962
4	3-PGA	0	mM	150	76.81159419	0.933152494
1	3-PGA	0	mM	300	81.01653401	0.98423658
2	3-PGA	0	mM	300	77.30149009	0.939104038
3	3-PGA	0	mM	300	89.50806289	1.087396675
4	3-PGA	0	mM	300	95.63176158	1.161790974
1	3-PGA	0	mM	600	100.3674219	1.219322565
2	3-PGA	0	mM	600	93.99877524	1.141952494
3	3-PGA	0	mM	600	98.32618904	1.194524466
4	3-PGA	0	mM	600	107.3076138	1.303636105
1	3-PGA	8	mM	10	4.062053519	0.049348219
2	3-PGA	8	mM	10	3.123086374	0.037941093
1	3-PGA	8	mM	50	17.53419065	0.213015677
2	3-PGA	8	mM	50	18.14656053	0.220455107
1	3-PGA	8	mM	150	43.66197183	0.530431354
2	3-PGA	8	mM	150	41.21249232	0.500673634
1	3-PGA	8	mM	300	67.50357214	0.820073159
2	3-PGA	8	mM	300	62.52296389	0.759565796
1	3-PGA	8	mM	600	76.77076953	0.932656532
2	3-PGA	8	mM	600	80.32251482	0.975805226
1	3-PGA	12	mM	10	1.571749351	0.019094537
2	3-PGA	12	mM	10	1.939171278	0.023558195
1	3-PGA	12	mM	50	12.02286184	0.146060808
2	3-PGA	12	mM	50	13.6558481	0.165899287
1	3-PGA	12	mM	150	28.0261278	0.34047791
2	3-PGA	12	mM	150	34.02735251	0.413384323
1	3-PGA	12	mM	300	50.07144313	0.608297387
2	3-PGA	12	mM	300	51.78607879	0.629127791
1	3-PGA	12	mM	600	64.84996934	0.787835629
2	3-PGA	12	mM	600	71.83098594	0.872645131
1	CA1P	0	uM	10	15.86037965	0.192681235
2	CA1P	0	uM	10	12.43110842	0.151020428
3	CA1P	0	uM	10	13.37007549	0.162427553
4	CA1P	0	uM	10	15.1255358	0.183753919
1	CA1P	0	uM	50	44.51928966	0.540846556
2	CA1P	0	uM	50	43.9885691	0.53439905
3	CA1P	0	uM	50	42.60053071	0.517536342
4	CA1P	0	uM	50	48.60175543	0.590442755
1	CA1P	0	uM	150	67.09532555	0.815113539
2	CA1P	0	uM	150	66.8503776	0.812137767
3	CA1P	0	uM	150	77.38313941	0.940095962
4	CA1P	0	uM	150	76.81159419	0.933152494
1	CA1P	0	uM	300	81.01653401	0.98423658
2	CA1P	0	uM	300	77.30149009	0.939104038
3	CA1P	0	uM	300	89.50806289	1.087396675
4	CA1P	0	uM	300	95.63176158	1.161790974
1	CA1P	0	uM	600	100.3674219	1.219322565
2	CA1P	0	uM	600	93.99877524	1.141952494
3	CA1P	0	uM	600	98.32618904	1.194524466
4	CA1P	0	uM	600	107.3076138	1.303636105
1	CA1P	0.6	uM	10	3.694631592	0.044884561
2	CA1P	0.6	uM	10	3.776280909	0.045876485
1	CA1P	0.6	uM	50	14.26821797	0.173338717
2	CA1P	0.6	uM	50	13.73749741	0.166891211
1	CA1P	0.6	uM	150	25.82159624	0.313695962
2	CA1P	0.6	uM	150	24.31108388	0.295345368
1	CA1P	0.6	uM	300	33.53745661	0.407432779
2	CA1P	0.6	uM	300	30.72055518	0.373211401
1	CA1P	0.6	uM	600	34.88467034	0.423799525
2	CA1P	0.6	uM	600	37.61992246	0.457028979
1	CA1P	1.5	uM	10	1.163502767	0.014134917
2	CA1P	1.5	uM	10	2.224943887	0.027029929
1	CA1P	1.5	uM	50	6.838130213	0.083073634
2	CA1P	1.5	uM	50	6.266584994	0.076130166
1	CA1P	1.5	uM	150	14.18656866	0.172346793
2	CA1P	1.5	uM	150	13.53337412	0.164411401
1	CA1P	1.5	uM	300	18.84057972	0.228886461
2	CA1P	1.5	uM	300	17.57501531	0.213511639
1	CA1P	1.5	uM	600	22.39232493	0.272035154
2	CA1P	1.5	uM	600	21.90242903	0.26608361
1	CABP	0	uM	10	15.86037965	0.192681235
2	CABP	0	uM	10	12.43110842	0.151020428
3	CABP	0	uM	10	13.37007549	0.162427553
4	CABP	0	uM	10	15.1255358	0.183753919
1	CABP	0	uM	50	44.51928966	0.540846556
2	CABP	0	uM	50	43.9885691	0.53439905
3	CABP	0	uM	50	42.60053071	0.517536342
4	CABP	0	uM	50	48.60175543	0.590442755
1	CABP	0	uM	150	67.09532555	0.815113539
2	CABP	0	uM	150	66.8503776	0.812137767
3	CABP	0	uM	150	77.38313941	0.940095962
4	CABP	0	uM	150	76.81159419	0.933152494
1	CABP	0	uM	300	81.01653401	0.98423658
2	CABP	0	uM	300	77.30149009	0.939104038
3	CABP	0	uM	300	89.50806289	1.087396675
4	CABP	0	uM	300	95.63176158	1.161790974
1	CABP	0	uM	600	100.3674219	1.219322565
2	CABP	0	uM	600	93.99877524	1.141952494
3	CABP	0	uM	600	98.32618904	1.194524466

Number	Sample	Met.conc	Unit	S_uM	Relative_Vel	Abs_Vel	Day
1	Control	0		8	102.52049435751644	0.44534433	1
2	Control	0		8	90.29732053941692	0.392247423	1
3	Control	0		8	91.39850733876756	0.397030928	1
4	Control	0		8	102.41037556247888	0.444865979	1
5	Control	0		8	109.01749658878768	0.47356701	1
6	Control	0		8	100.5383581186853	0.436734021	1
7	Control	0		8	94.09641493962533	0.408750515	1
8	Control	0		8	100.70353596593415	0.437451546	1
9	Control	0		8	109.01749658878768	0.47356701	1
10	2-PG 28 uM	28	uM	8	97.74652549396825	0.424606428	1
11	2-PG 28 uM	28	uM	8	104.39251203151501	0.453476289	1
12	Glycolate 30 uM	30	uM	8	93.16040621772855	0.404684536	1
13	Glycolate 30 uM	30	uM	8	102.0800196377762	0.443430928	1
14	Glyoxylate 80 uM	80	uM	8	82.69913127859004	0.359241237	1
15	Glyoxylate 80 uM	80	uM	8	98.66634044468672	0.428602062	1
16	GAP 80 uM	80	uM	8	98.22586572494647	0.42668866	1
17	GAP 80 uM	80	uM	8	89.41637109993643	0.388420619	1
18	DHAP 2.4 mM	2.4	mM	8	114.74366817561597	0.498441237	1
19	DHAP 2.4 mM	2.4	mM	8	111.88058249730433	0.486004124	1
20	Gln 80 uM	80	uM	8	98.33598428977903	0.42716701	1
21	Gln 80 uM	80	uM	8	93.60088093746882	0.406597938	1
22	Gly 80 uM	80	uM	8	105.49369883086565	0.458259794	1
23	Gly 80 uM	80	uM	8	99.43717108912966	0.431950515	1
24	Control	0		8	97.52682677567705	0.5442418879056047	2
25	Control	0		8	99.9795605518651	0.557929203539823	2
26	Control	0		8	102.49361267245784	0.5719587020648967	2
27	Malate 40 uM	40	uM	8	91.51762902401637	0.5107079646017699	2
28	Malate 40 uM	40	uM	8	91.94685743484928	0.5131032448377582	2
29	Malate 40 uM	40	uM	8	105.49821154828823	0.5887256637168142	2
30	ketog 1.5uM	1.5	uM	8	105.6208482370976	0.5894100294985251	2
31	ketog 1.5uM	1.5	uM	8	108.25753704649976	0.6041238938053097	2
32	ketog 1.5uM	1.5	uM	8	112.36586612161474	0.6270501474926254	2
33	OAA 1.5uM	1.5	uM	8	101.26724578436384	0.5651150442477876	2
34	OAA 1.5uM	1.5	uM	8	109.60654062340318	0.6116519174041298	2
35	OAA 1.5uM	1.5	uM	8	103.41338783852838	0.5770914454277287	2
36	Glu 80uM	80	uM	8	92.37608584568217	0.5154985250737463	2
37	Glu 80uM	80	uM	8	110.8942258559019	0.6188377581120944	2
38	Glu 80uM	80	uM	8	102.86152273888607	0.5740117994100296	2
39	G6P 6 mM	0		8	100.52910052910056	0.5235154394299288	3
40	G6P 6 mM	0		8	99.47089947089948	0.5180047505938242	3
41	G6P 6 mM	6	mM	8	92.69841269841271	0.4827363420427554	3
42	G6P 6 mM	6	mM	8	85.71428571428571	0.44636579572446555	3
43	F6P 6 mM	0		8	96.6819625838334	0.4367340206185567	4
44	F6P 6 mM	0		8	104.83586304271091	0.4735670103092785	4
45	F6P 6 mM	0		8	98.48217437345572	0.4448659793814435	4
46	F6P 6 mM	6	mM	8	80.5859512883869	0.36402474226804143	4
47	F6P 6 mM	6	mM	8	71.79668196258385	0.32432164948453623	4

Number	Sample	Met.conc	Unit	S_uM	Relative_Vel	Abs_Vel
1	Control	0		50	96.28986419	0.626092035
2	Control	0		50	98.2317651	0.638718584
3	Control	0		50	105.4783707	0.685837168
4	Control	0		50	100.20717286979908	0.651563
5	Control	0		50	100.15934260509329	0.651252
6	Control	0		50	96.84454756	0.6296986746278423
7	Control	0		50	110.487239	0.7184056273545244
8	2-PG	28	uM	50	92.69024321	0.602686726
9	2-PG	28	uM	50	90.98515949	0.5916
10	2-PG	28	uM	50	95.34259549	0.619932743
11	Glycolate	30	uM	50	96.19513735	0.625476106
12	Glycolate	30	uM	50	91.60088417	0.59560354
13	Glycolate	30	uM	50	97.99494792	0.637178761
14	Glyoxylate	80	uM	50	101.8787496	0.662431858
15	Glyoxylate	80	uM	50	92.50078938	0.601454867
16	Glyoxylate	80	uM	50	101.0735712	0.65719646
17	GAP	80	uM	50	89.51689293	0.582053097
18	GAP	80	uM	50	88.52226089	0.575585841
19	GAP	80	uM	50	95.34259549	0.619932743
20	DHAP	2.4	mM	50	98.42121878	0.639950442
21	DHAP	2.4	mM	50	93.02178723	0.604842478
22	DHAP	2.4	mM	50	93.44805808	0.607614159
23	F6P	6	mM	50	83.88064417	0.5454053097345133
24	F6P	6	mM	50	81.27565522	0.5284672566371682
25	F6P	6	mM	50	89.51689299	0.5820530973451328
26	G6P	6	mM	50	83.91631252	0.5456372310372671
27	G6P	6	mM	50	85.0931677	0.5532893308881989
28	Ketoglutarate	1.5	uM	50	100.7893907	0.655348673
29	Ketoglutarate	1.5	uM	50	96.43195453	0.627015929
30	Ketoglutarate	1.5	uM	50	94.01641943	0.611309735
31	Malate	40	uM	50	97.04767922	0.631019469
32	Malate	40	uM	50	97.80549424	0.635946903
33	Malate	40	uM	50	96.43195453	0.627015929
34	OAA	1.5	uM	50	97.42658673	0.633483186
35	OAA	1.5	uM	50	92.07451853	0.598683186
36	OAA	1.5	uM	50	96.81086204	0.629479646
37	Gln	80	uM	50	89.8484371	0.58420885
38	Gln	80	uM	50	90.13261762	0.586056637
39	Gln	80	uM	50	98.75276295	0.642106195
40	Glu	80	uM	50	87.47099768	0.5687503601693734
41	Glu	80	uM	50	96.28770302	0.6260779826798143
42	Gly	80	uM	50	94.39532679	0.613773451
43	Gly	80	uM	50	93.44805808	0.607614159
44	Gly	80	uM	50	100.3157563	0.652269027

Equation 1	Number	Met	Met.conc	Vmax	Km	Average	α	Ki app	Average	SE	Equation 2		Competitive-Like	(Vmax-v)/v=(1+[I]/Ki)Km/[S]		Ki app	Average	SE
	1	3PGA	0	1.31	79.49	75.73						1	3PGA-12 mM	(Vmax-v)/v=	0.6945159000926574	2.6651452455236253	3.2088664513521654	0.5437212058285397
	2	3PGA	0	1.23	71.88							2	3PGA-12 mM	(Vmax-v)/v=	0.5298314888552332	3.7525876571807055
	3	3PGA	0	1.35	74.87									Km/[S]=	0.12621666666666667
	4	3PGA	0	1.45	76.68				mM					[I]=	12
	5	3PGA	8	1.31	213.22		2.8155288525023106	4.406429558513347	3.3582323785361106	0.3542240686665536
	6	3PGA	8	1.42	272.65		3.6002905057440904	3.0765793215519					Noncompetitive-Like	(Vmax-v)/v=Km/[S]+(Km/[S]+1)[I]/Ki		Ki app	Average	SE
	7	3PGA	12	1.34	395.17		5.218143404199129	2.844853493613824				1	CA1P-1.5 uM	(Vmax-v)/v=	3.9074539829510413	0.44676513497968223	0.44046346812283915	0.00630166685684308
	8	3PGA	12	1.41	368.4		4.864650732866763	3.105067140465371				2	CA1P-1.5 uM	(Vmax-v)/v=	4.017219963304015	0.4341618012659961
	9	CA1P	0	1.31	79.49	1.335								Km/[S]=	0.12621666666666667
	10	CA1P	0	1.23	71.88									[I]=	1.5
	11	CA1P	0	1.35	74.87
	12	CA1P	0	1.45	76.68				uM			1	CABP-0.2 uM	(Vmax-v)/v=	2.106449515312317	0.11374588270636206	0.10211557828630413	0.011630304420057963
	13	CA1P	0.6	0.5	90.74		2.67	0.3592814371257485	0.4448595302917683	0.039763840524283674		2	CA1P-0.2 uM	(Vmax-v)/v=	2.6154983332755806	0.0904852738662462
	14	CA1P	0.6	0.53	119.25		2.5188679245283017	0.39503105590062115						Km/[S]=	0.12621666666666667
	15	CA1P	1.5	0.34	153.64		3.926470588235294	0.5125628140703518						[I]=	0.2
	16	CA1P	1.5	0.34	161.83		3.926470588235294	0.5125628140703518
	17	CABP	0	1.31	79.49	1.335
	18	CABP	0	1.23	71.88
	19	CABP	0	1.35	74.87
	20	CABP	0	1.45	76.68				uM
	21	CABP	0.15	0.55	47.07		2.427272727272727	0.10509554140127392	0.11787121979799667	0.011907683907824653
	22	CABP	0.15	0.62	79.79		2.153225806451613	0.13006993006993006
	23	CABP	0.2	0.56	117.44		2.383928571428571	0.1445161290322581
	24	CABP	0.2	0.42	68.12		3.178571428571429	0.09180327868852459