Selected Cell
Cell:
Value:
Pi IC50 with 3-PGA
2-PGA vs 3-PGA
3-PGA Fru-6P EC50
GlgC G1P MM
3-PGA-Pi interplay
GlgC G1P MM M. Green
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| Activity (U/mg) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Pi (mM) | No 3-PGA | + 0.5 mM 3-PGA | + 1 mM 3-PGA | + 1.25 mM 3-PGA | + 1.75 mM 3-PGA | + 2 mM 3-PGA | + 2.25 mM 3-PGA | + 2.5 mM 3-PGA | + 4 mM 3-PGA | |||||||||||||||||||||||||||||||||||||||||||||||
| 5 | -0.01 | -0.09 | -0.09 | -0.04 | -0.03 | -0.12 | -0.03 | -0.09 | -0.03 | -0.08 | -0.16 | -0.11 | -0.12 | -0.11 | -0.16 | -0.1 | -0.09 | -0.05 | -0.03 | -0.06 | -0.04 | -0.12 | -0.04 | -0.03 | -0.05 | 0.06 | -0.03 | -0.08 | -0.06 | -0.04 | ||||||||||||||||||||||||||
| 2 | -0.12 | -0.1 | -0.12 | -0.16 | -0.15 | -0.11 | 0.07 | 0.01 | 0.02 | 0.05 | 0.03 | -0.01 | 0.06 | 0.04 | 0.02 | 0.03 | -0.03 | 0.09 | 0.09 | 0.1 | 0.15 | 0.03 | 0.02 | 0.06 | 0.24 | 0.17 | 0.24 | 0.21 | 0.3 | 0.39 | 0.94 | 1 | 0.75 | 0.61 | 0.35 | 0.77 | ||||||||||||||||||||
| 1 | -0.02 | 0.02 | 0.04 | 0.06 | 0.06 | 0.11 | 0.38 | 0.32 | 0.38 | 0.08 | 0.13 | 0.09 | 0.25 | 0.39 | 0.21 | 0.73 | 0.5 | 1.14 | 1.63 | 1.5 | 1.3 | 0.66 | 0.86 | 1.26 | 2.37 | 2.04 | 2.5 | 2.32 | 2.5 | 2.74 | 4.08 | 4.04 | 3.81 | 2.36 | 2.58 | 2.79 | ||||||||||||||||||||
| 0.8 | 0.7 | 0.58 | 0.73 | 0.24 | 0.22 | 0.19 | 0.5 | 0.84 | 0.4 | 1.62 | 1.27 | 1.9 | 2.42 | 2.18 | 1.99 | 1.67 | 1.94 | 2.5 | 3.03 | 2.61 | 3.14 | 2.91 | 3.11 | 3.3 | 4.26 | 4.17 | 4.03 | 2.53 | 2.76 | 2.89 | ||||||||||||||||||||||||||
| 0.6 | 0.1 | 0.14 | 0.15 | 0.34 | 0.33 | 0.42 | 1.39 | 1.18 | 1.53 | 0.5 | 0.51 | 0.44 | 1.27 | 1.6 | 1.11 | 2.82 | 2.5 | 2.66 | 2.93 | 2.77 | 2.61 | 3.03 | 3.18 | 3.45 | 3.41 | 3.14 | 3.46 | 3.26 | 3.41 | 3.65 | 4.21 | 4.24 | 4.12 | 2.64 | 2.92 | 3.02 | ||||||||||||||||||||
| 0.2 | 0.47 | 0.5 | 0.57 | 1.7 | 1.52 | 1.65 | 2.88 | 2.61 | 2.96 | 3.39 | 3.39 | 3.37 | 3.71 | 3.06 | 3.4 | 3.75 | 3.57 | 3.33 | 3.28 | 3.21 | 3.08 | 3.9 | 3.93 | 4.14 | 3.77 | 3.5 | 3.71 | 3.57 | 3.69 | 3.84 | 4.34 | 4.31 | 4.17 | 2.73 | 3.05 | 3.18 | ||||||||||||||||||||
| 0.1 | 0.58 | 0.61 | 0.69 | 2.02 | 1.8 | 1.92 | 3.07 | 2.77 | 3.12 | 3.71 | 3.79 | 3.79 | 3.83 | 3.18 | 3.58 | 3.86 | 3.61 | 3.38 | 3.35 | 3.28 | 3.13 | 3.96 | 4.01 | 4.16 | 3.86 | 3.54 | 3.75 | 3.63 | 3.69 | 3.86 | 4.39 | 4.34 | 4.2 | 2.78 | 3.13 | 3.16 | ||||||||||||||||||||
| 0.05 | 0.64 | 0.65 | 0.73 | 2.18 | 1.91 | 2.04 | ||||||||||||||||||||||||||||||||||||||||||||||||||
| 0.025 | 0.64 | 0.68 | 0.76 | 2.22 | 1.96 | 2.1 | ||||||||||||||||||||||||||||||||||||||||||||||||||
| 0 | 0.68 | 0.7 | 0.78 | 2.3 | 2.01 | 2.17 | 3.16 | 2.92 | 3.21 | 3.9 | 3.85 | 3.97 | 3.93 | 3.27 | 3.72 | 3.96 | 3.74 | 3.47 | 3.46 | 3.4 | 3.22 | 4.08 | 4.05 | 4.24 | 3.89 | 3.57 | 3.81 | 3.7 | 3.8 | 3.94 | 4.4 | 4.29 | 4.19 | 2.91 | 3.18 | 3.2 | ||||||||||||||||||||
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| Activity (U/mg) | ||||||||||||
| 2-PGA (mM) | No 3-PGA | + 0.25 mM 3-PGA | + 0.5 mM 3-PGA | + 1 mM 3-PGA | ||||||||
| 1.0E-5 | 0.74 | 0.68 | 0.68 | 1.03 | 0.97 | 0.93 | 2.38 | 2.33 | 2.24 | 2.84 | 2.97 | 2.92 |
| 0.1 | 0.73 | 0.68 | 0.7 | 0.98 | 0.92 | 0.9 | 2.33 | 2.22 | 2.22 | 2.78 | 2.82 | 2.89 |
| 0.2 | 0.7 | 0.68 | 0.67 | 0.96 | 0.92 | 0.92 | 2.25 | 2.2 | 2.16 | 2.73 | 2.82 | 2.85 |
| 0.6 | 0.67 | 0.63 | 0.63 | 0.91 | 0.88 | 0.85 | 2.12 | 2.04 | 2.04 | 2.61 | 2.72 | 2.76 |
| 0.8 | 0.6 | 0.6 | 0.62 | 0.84 | 0.8 | 0.81 | 2.05 | 1.94 | 1.98 | 2.57 | 2.64 | 2.66 |
| 1 | 0.59 | 0.59 | 0.59 | 0.81 | 0.77 | 0.76 | 1.95 | 1.87 | 1.9 | 2.53 | 2.61 | 2.64 |
| 2 | 0.53 | 0.5 | 0.55 | 0.67 | 0.63 | 0.68 | 1.64 | 1.53 | 1.56 | 2.3 | 2.33 | 2.36 |
| 4 | 0.37 | 0.35 | 0.38 | 0.48 | 0.44 | 0.44 | 1.1 | 1.01 | 1.06 | 1.77 | 1.92 | 1.88 |
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| 3-PGA (mM) | Activity (U/mg) | ||||||||
| 8 | 3.89 | 3.61 | 3.57 | ||||||
| 4 | 4.06 | 3.84 | 3.88 | ||||||
| 2 | 4.13 | 3.84 | 3.88 | ||||||
| 1 | 3.83 | 3.53 | 3.57 | ||||||
| 0.5 | 2.71 | 2.52 | 2.55 | ||||||
| 0.25 | 1.23 | 1.18 | 1.25 | ||||||
| 0.125 | 0.78 | 0.71 | 0.75 | ||||||
| 0 | 0.741083 | 0.681191 | 0.731423 | ||||||
| Fru-6P (mM) | Activity (U/mg) | ||||||||
| 20 | 1.78 | 1.7 | 1.72 | ||||||
| 10 | 1.61 | 1.6 | 1.61 | ||||||
| 5 | 1.5 | 1.48 | 1.47 | ||||||
| 2.5 | 1.28 | 1.27 | 1.26 | ||||||
| 1.25 | 1 | 0.98 | 0.97 | ||||||
| 0.625 | 0.83 | 0.82 | 0.79 | ||||||
| 0.3125 | 0.74 | 0.75 | 0.72 | ||||||
| 0 | 0.7 | 0.66 | 0.63 | ||||||
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| Glucose-1-Phosphate (mM) | GlgC Activity (U/mg) | GlgC-Long Activity (U/mg) | ||||||||
| 2 | 5.75 | 5.54 | 5.38 | 6.14 | 5.87 | 5.82 | 1.79 | 1.87 | 1.61 | |
| 1 | 5.26 | 5.02 | 4.95 | 5.86 | 5.62 | 5.61 | 1.59 | 1.69 | 1.49 | |
| 0.5 | 4.58 | 4.42 | 4.37 | 4.91 | 4.76 | 4.7 | 1.52 | 1.58 | 1.38 | |
| 0.25 | 3.23 | 3.22 | 3.18 | 3.28 | 3.38 | 3.21 | 1.35 | 1.44 | 1.24 | |
| 0.125 | 1.79 | 1.79 | 1.79 | 1.7 | 1.74 | 1.68 | 1.11 | 1.16 | 1.08 | |
| 0.0625 | 0.89 | 0.86 | 0.87 | |||||||
| 0.03125 | 0.42 | 0.35 | 0.37 | |||||||
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
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| Pi (mM) | EC50 3-PGA (mM) | 3-PGA (mM) | IC50 Phosphate (mM) | ||||||
| 0 | 0.43 | 0 | 0.42 | ||||||
| 0.1 | 0.51 | 0.5 | 0.36 | ||||||
| 0.2 | 0.71 | 1 | 0.43 | ||||||
| 0.6 | 1.46 | 1.25 | 0.52 | ||||||
| 0.8 | 1.98 | 1.75 | 0.77 | ||||||
| 1 | 2.03 | 2 | 0.87 | ||||||
| 2 | 2.75 | 2.25 | 1.1 | ||||||
| 2.5 | 1.2 | ||||||||
| 4 | 1.5 | ||||||||
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| Glucose-1-Phosphate (mM) | Activity GlgC (U/mg) | Activity GlgC-Long (U/mg) | |||||||
| 1 | 7.5 | 6.08 | 6 | 7.25 | 1.75 | 0.75 | 1.6 | ||
| 0.5 | 7.25 | 5.15 | 5.25 | 7 | 1.5 | 0.7 | 1.5 | ||
| 0.25 | 3.75 | 3.13 | 4 | 4.5 | 1.75 | 0.93 | 1.6 | ||
| 0.125 | 1.75 | 1.55 | 1.75 | 2 | 1.25 | 0.78 | 1.12 | ||
| 0.0625 | 0.75 | 0.58 | 0.62 | ||||||
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
