Selected Cell
Cell:
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A.SCXP
A.SCCL
D.BSRA
D.GLYN
D.ELSA
D.ADNKA
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| UID | Name | Scientist | Formula | Lab | Form | Manufacturer | ProductNumber | CASNumber | MDLNumber | StorageTempMin | StorageTempMax | StorageTempUnits |
| CHM-220929ALT-1 | Bovine Liver Glycogen | Eddie Irvine | (C6H10O5)n | Alter Lab | powder | Sigma-Aldrich | G0885 | 9005-79-2 | MFCD00081547 | 2 | 8 | Celsius |
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| UID | Name | Catalog# | Vendor | Analyte | Clone | Fc-variant | AminoAcidMutations | Description | Protocol | Scientist | Notes |
| AB-220920ALT-1 | MAb 24c5 | 4BFab5-Ab-BNP4 | Hytest | IgG1 | 24c5 | https://fairdomhub.org/sops/724 | Eddie Irvine | ||||
| AB-220921ALT-1 | Fc Variant IgG1 | IgG1 | 24c5 | IgG1 | human IgG1 | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111768/ | |||
| AB-220920ALT-2 | Fc Variant IgG2 | IgG2 | 24c5 | IgG2 | human IgG2 | https://fairdomhub.org/sops/724 | Eddie Irvine | ||||
| AB-220920ALT-3 | Fc Variant IgG4 | IgG4 | 24c5 | IgG4 | human IgG4 | https://fairdomhub.org/sops/724 | Eddie Irvine | ||||
| AB-220920ALT-4 | Fc Variant LALA | IgG1 | 24c5 | LALA | L234A/L235A | Lower FcyR binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111768/ | ||
| AB-220920ALT-5 | Fc Variant LALA LS | IgG1 | 24c5 | LALA LS | L234A/L235A/M428L/N434S | Lower FcyR binding; Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-6 | Fc Variant N297Q | IgG1 | 24c5 | N297Q | N297Q | Lower FcyR binding | https://fairdomhub.org/sops/724 | Eddie Irvine | |||
| AB-220920ALT-7 | Fc Variant AA LS | IgG1 | 24c5 | AA LS | T307A/E380A/M428L/N434S | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-8 | Fc Variant QL | IgG1 | 24c5 | QL | T250Q/M428L | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/14699147/ | ||
| AB-220920ALT-9 | Fc Variant QLS | IgG1 | 24c5 | QLS | T250Q/M428L/N434S | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-10 | Fc Variant YTE | IgG1 | 24c5 | YTE | M252Y/S254T/T256E | Lower ADCC; Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/12391234/ | ||
| AB-220920ALT-11 | Fc Variant YTE LS | IgG1 | 24c5 | YTE LS | M252Y/S254T/T256E/M428L/N434S | Lower ADCC; Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/12391234/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-12 | Fc Variant E380A | IgG1 | 24c5 | E380A | E380A | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | |||
| AB-220920ALT-13 | Fc Variant E380A LS | IgG1 | 24c5 | E380A LS | E380A/M428L/N434S | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/11096108/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-14 | Fc Variant AAA | IgG1 | 24c5 | AAA | T307A/E380A/N434A | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/11096108/ | ||
| AB-220920ALT-15 | Fc Variant DVNH | IgG1 | 24c5 | DVNH | D376V/N434H | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/17050651/ | ||
| AB-220920ALT-16 | Fc Variant MLNS | IgG1 | 24c5 | MLNS | M428L/N434S | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | |||
| AB-220920ALT-17 | Fc Variant N434A | IgG1 | 24c5 | N434A | N434A | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/11096108/ | ||
| AB-220920ALT-18 | Fc Variant N434W | IgG1 | 24c5 | N434W | N434W | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/19494290/ | ||
| AB-220920ALT-19 | Fc Variant PINH | IgG1 | 24c5 | PINH | P257I/N434H | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/17050651/ | ||
| AB-220920ALT-20 | Fc Variant P257I LS | IgG1 | 24c5 | P257I LS | P257I/M428L/N434S | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-21 | Fc Variant PIQI | IgG1 | 24c5 | PIQI | P257I/Q311I | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/17050651/ | ||
| AB-220920ALT-22 | Fc Variant PIQI LS | IgG1 | 24c5 | PIQI LS | P257I/Q311I/M428L/N434S | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-23 | Fc Variant D376V LS | IgG1 | 24c5 | D376V LS | D376V/M428L/N434S | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/; https://pubmed.ncbi.nlm.nih.gov/17050651/ | ||
| AB-220920ALT-24 | Fc Variant SELF | IgG1 | 24c5 | SELF | S267E/L328F | Higher FcyRIIb binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/18691763/ | ||
| AB-220920ALT-25 | Fc Variant SELF LS | IgG1 | 24c5 | SELF LS | S267E/L328F/M428L/N434S | Higher FcyRIIb binding; Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/18691763/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-26 | Fc Variant IgG3RH | IgG1 | 24c5 | IgG3 RH | R435H | Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | |||
| AB-220920ALT-27 | Fc Variant SAEAKA | IgG1 | 24c5 | SAEAKA | S298A/E333A/K334A | Higher ADCC | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1389705/; https://pubmed.ncbi.nlm.nih.gov/11096108/ | ||
| AB-220920ALT-28 | Fc Variant S324T | IgG1 | 24c5 | S324T | S324T | Higher Complement | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2840237/ | ||
| AB-220920ALT-29 | Fc Variant S324T LS | IgG1 | 24c5 | S324T LS | S324T/M428L/N434S | Higher Complement; Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2840237/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-30 | Fc Variant HFST | IgG1 | 24c5 | HFST | H268F/S324T | Higher Complement | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2840237/ | ||
| AB-220920ALT-31 | Fc Variant HFST LS | IgG1 | 24c5 | HFST LS | H268F/S324T/M428L/N434S | Higher Complement; Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2840237/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-32 | Fc Variant E345R LS | IgG1 | 24c5 | E345R LS | E345R/M428L/N434S | Higher Complement; Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250092/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-33 | Fc Variant SEHFST LS | IgG1 | 24c5 | SEHFST LS | S267E/H268F/S324T/M428L/N434S | Higher Complement; Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2840237/ | ||
| AB-220920ALT-34 | Fc Variant KWES | IgG1 | 24c5 | KWES | K236W/E333S | Higher Complement | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/7543517/ | ||
| AB-220920ALT-35 | Fc Variant RGYLS | IgG1 | 24c5 | RGYLS | E345R/E430G/S440Y/M428L/N434S | Higher Complement; Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250092/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-36 | Fc Variant ALS | IgG1 | 24c5 | ALS | E333A/M428L/N434S | Higher Complement; Higher ADCC; Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-37 | Fc Variant EFTEA | IgG1 | 24c5 | EFTEA | G236A/S267E/H268F/S324T/I332E | Higher ADCC; Higher Phagocytosis; Higher Complement | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2840237/ | ||
| AB-220920ALT-38 | Fc Variant EFTEA LS | IgG1 | 24c5 | EFTEA LS | G236A/S267E/H268F/S324T/I332E/M428L/N434S | Higher ADCC; Higher Phagocytosis; Higher Complement; Higher FcRn binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2840237/ | ||
| AB-220920ALT-39 | Fc Variant I332E | IgG1 | 24c5 | I332E | I332E | Higher ADCC; Higher Phagocytosis | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1389705/ | ||
| AB-220920ALT-40 | Fc Variant I332E LS | IgG1 | 24c5 | I332E LS | I332E/M428L/N434S | Higher ADCC; Higher Phagocytosis; Higher FcRN binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1389705/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-41 | Fc Variant SDIE | IgG1 | 24c5 | SDIE | S239D/I332E | Higher ADCC; Higher Phagocytosis | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1389705/; https://pubmed.ncbi.nlm.nih.gov/18723496/ | ||
| AB-220920ALT-42 | Fc Variant SDIE LS | IgG1 | 24c5 | SDIE LS | S239D/I332E/M428L/N434S | Higher ADCC; Higher Phagocytosis; Higher FcRN binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1389705/; https://pubmed.ncbi.nlm.nih.gov/18723496/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-43 | Fc Variant SDIEAL | IgG1 | 24c5 | SDIEAL | S239D/A330L/I332E | Higher ADCC; Higher Phagocytosis | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1389705/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187489/ | ||
| AB-220920ALT-44 | Fc Variant SDIEAL LS | IgG1 | 24c5 | SDIEAL LS | S2392D/A330L/I332E/M428L/N434S | Higher ADCC; Higher Phagocytosis; Higher FcRN binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1389705/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187489/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-45 | Fc Variant SDIEGA | IgG1 | 24c5 | SDIEGA | G236A/S239D/I332E | Higher ADCC; Higher Phagocytosis | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187489/; https://pubmed.ncbi.nlm.nih.gov/18723496/ | ||
| AB-220920ALT-46 | Fc Variant SDIEGA LS | IgG1 | 24c5 | SDIEGA LS | G236A/S239D/I332E/M428L/N434S | Higher ADCC; Higher Phagocytosis; Higher FcRN binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187489/; https://pubmed.ncbi.nlm.nih.gov/18723496/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-47 | Fc Variant SDIESA | IgG1 | 24c5 | SDIESA | S239D/S298A/I332E | Higher ADCC; Higher Phagocytosis | https://fairdomhub.org/sops/724 | Eddie Irvine | |||
| AB-220920ALT-48 | Fc Variant SDIESA LS | IgG1 | 24c5 | SDIESA LS | S239D/S298A/I332E/M428L/N434S | Higher ADCC; Higher Phagocytosis; Higher FcRN binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-49 | Fc Variant SDIEALGA | IgG1 | 24c5 | SDIEALGA | G236A/S239D/A330L/I332E | Higher ADCC; Higher Phagocytosis | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3341029/ | ||
| AB-220920ALT-50 | Fc Variant SDIEALGA LS | IgG1 | 24c5 | SDIEALGA LS | G236A/S239D/A330L/I332E/M428L/N434S | Higher ADCC; Higher Phagocytosis; Higher FcRN binding | https://fairdomhub.org/sops/724 | Eddie Irvine | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3341029/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855492/ | ||
| AB-220920ALT-51 | Fc Variant K326W | IgG1 | 24c5 | K326W | K326W | Higher Complement; Higher ADCC | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/10754313/; https://pubmed.ncbi.nlm.nih.gov/11160318/ | ||
| AB-220920ALT-52 | Fc Variant E333A | IgG1 | 24c5 | E333A | E333A | Higher Complement; Higher ADCC | https://fairdomhub.org/sops/724 | Eddie Irvine | https://pubmed.ncbi.nlm.nih.gov/10754313/; https://pubmed.ncbi.nlm.nih.gov/11160318/ | ||
| AB-220921ALT-2 | Fc Variant IgG1 Isotype | IgG1 | 24c5 | IgG1 Isotype | https://fairdomhub.org/sops/724 | Eddie Irvine |
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| UID | Name | Species | Strain | TaxonomyID | Phenotype | Morphology | GramStaining | Scientist | Notes |
| BAC-240502ALT-1 | H37Rv Mtb Live/dead Reporter | Mycobacterium tuberculosis | H37Rv | 83332 | Constitutive mCherry; Inducible GFP | Bacillus | Positive | Eddie Irvine | https://doi.org/10.1016/j.chom.2012.06.010 |
| BAC-240502ALT-2 | H37Rv Mtb | Mycobacterium tuberculosis | H37Rv | 83332 | N/A | Bacillus | Positive | Eddie Irvine | https://doi.org/10.1099/00207713-22-2-99 |
| BAC-240502ALT-3 | H37Rv Mtb-Lux | Mycobacterium tuberculosis | H37Rv | 83332 | Luminescent | Bacillus | Positive | Eddie Irvine | https://doi.org/10.1371/journal.pone.0010777; https://doi.org/10.1371/journal.pone.0010777 |
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| UID | Name | Scientist | Age | Sex | DiseaseReference | DiseaseStage | Ethnicity | Race | SubjectLocation | Notes | ||||||||||||||||||||||||||||
| PAT-240502ALT-1 | Multiple Anonymous Blood Donors | Eddie Irvine | NA | NA | HIV | Healthy | NA | NA | United States of America | Samples from multiple independent and anonymous donors were used to generate all the downstream data. Given the lack of patient metadata, we make no distinction between donors and have linked everything to a single phantom donor. | ||||||||||||||||||||||||||||
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| UID | Name | Parent | Scientist | Type | Notes | ||||||||||||
| PAV-240502ALT-1 | Multiple Anonymous Donor Visits | PAT-240502ALT-1 | Eddie Irvine | Whole Blood | Samples from multiple independent and anonymous donors were used to generate all the downstream data. Given the lack of patient metadata, we make no distinction between donors and have linked everything to a single phantom donor. | ||||||||||||
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| UID | Name | Scientist | Parent | Type | Notes | ||||
| TIS-240502ALT-1 | Anonymous Blood Sample - BSRA/ABFP | Eddie Irvine | PAV-240502ALT-1 | Whole Blood | Samples from multiple independent and anonymous donors were used to generate all the downstream data. Given the lack of patient metadata, we make no distinction between donors and have linked everything to a single phantom donor. | ||||
| TIS-240502ALT-2 | WBA Supernatant | Eddie Irvine | D.BSRA-240502ALT-2; D.BSRA-240502ALT-3 | Whole Blood | "At the final timepoint of the whole-blood restriction assay (120hr), the assay plates were centrifuged at 800xg for 5min, then 110uL of supernatant was collected from each well and transferred into a separate 96-well plate which was stored at -20C until further use." | ||||
| TIS-240606ALT-1 | Anonymous Blood Sample - SEHFST LS | Eddie Irvine | PAV-240502ALT-1; AB-220920ALT-33; BAC-240502ALT-2 | Whole Blood | Samples from multiple independent and anonymous donors were used to generate all the downstream data. Given the lack of patient metadata, we make no distinction between donors and have linked everything to a single phantom donor. | ||||
| TIS-240606ALT-2 | Anonymous Blood Sample - BAC Control | Eddie Irvine | PAV-240502ALT-1; BAC-240502ALT-2 | Whole Blood | Samples from multiple independent and anonymous donors were used to generate all the downstream data. Given the lack of patient metadata, we make no distinction between donors and have linked everything to a single phantom donor. | ||||
| TIS-240606ALT-3 | Anonymous Blood Sample - Control | Eddie Irvine | PAV-240502ALT-1 | Whole Blood | Samples from multiple independent and anonymous donors were used to generate all the downstream data. Given the lack of patient metadata, we make no distinction between donors and have linked everything to a single phantom donor. | ||||
| TIS-240606ALT-4 | Anonymous Blood Sample - IgG1 | Eddie Irvine | PAV-240502ALT-1; AB-220921ALT-1; BAC-240502ALT-2 | Whole Blood | Samples from multiple independent and anonymous donors were used to generate all the downstream data. Given the lack of patient metadata, we make no distinction between donors and have linked everything to a single phantom donor. | ||||
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| UID | Name | Protocol | Scientist | Parent | Type | LibraryType | Barcode | BarcodeType | Barcode2 | Barcode2Type | NumPrepCycles | Notes | Lab |
| DNA-240604ALT-1 | EI-DNA-SEHFST LS | https://fairdomhub.org/sops/733 | Eddie Irvine | TIS-240606ALT-1 | Illumina Library | mRNA | LMO anchor | MULTI-seq | LMO co-anchor | MULTI-seq | 150 | sequenced on Illumina NextSeq500 | Alter |
| DNA-240606ALT-1 | EI-DNA-BAC Control | https://fairdomhub.org/sops/733 | Eddie Irvine | TIS-240606ALT-2 | Illumina Library | mRNA | LMO anchor | MULTI-seq | LMO co-anchor | MULTI-seq | 150 | sequenced on Illumina NextSeq500 | Alter |
| DNA-240606ALT-2 | EI-DNA-Control | https://fairdomhub.org/sops/733 | Eddie Irvine | TIS-240606ALT-3 | Illumina Library | mRNA | LMO anchor | MULTI-seq | LMO co-anchor | MULTI-seq | 150 | sequenced on Illumina NextSeq500 | Alter |
| DNA-240606ALT-3 | EI-DNA-IgG1 | https://fairdomhub.org/sops/733 | Eddie Irvine | TIS-240606ALT-4 | Illumina Library | mRNA | LMO anchor | MULTI-seq | LMO co-anchor | MULTI-seq | 150 | sequenced on Illumina NextSeq500 | Alter |
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| UID | Name | File_PrimaryData | Link_PrimaryData | Scientist | DataType | LibraryPrep | Barcodes | BarcodesDataType | Genes | GenesDataType | Metadata | MetadataDataType | ReferenceGenome | ReferenceGenomeSource | Pipeline | Protocol | Parent | Lab | Repository | Accession | Checksum_PrimaryData | Checksum_Barcodes | Checksum_Genes | Checksum_Metadata | Checksum_PrimaryType |
| A.SCXP-240604ALT-1 | EI-A.SCXP-1-raw | Lane1_raw_feature_bc_matrix.h5.txt | https://singlecell.broadinstitute.org/single_cell/study/SCP2670/fc-engineered-antibodies-leverage-neutrophils-to-drive-control-of-mycobacterium-tuberculosis#study-download | Eddie Irvine | H5 | 10x, Single Cell 3' v3 | Lane1_raw_barcodes.tsv | TSV | Lane1_raw_features.tsv | TSV | Lane1_metrics_summary.csv | CSV | GRCh38-2020-A | /mnt/enterprise/references/refdata-gex-GRCh38-2020-A | cellranger-6.1.2 | https://fairdomhub.org/sops/733 | DNA-240604ALT-1; DNA-240606ALT-1; DNA-240606ALT-2; DNA-240606ALT-3 | Alter | Single Cell Portal | SCP2670 | f07375d3e6b36784aa2e2f3ebf1ecd75 | f52df2543db1dd83829b0d4825f8b743 | 283dc7ac5095a6dfbf8267dc1359757d | 5bdecc12045a72a9ff70aa7632ab867c | MD5 |
| A.SCXP-240604ALT-2 | EI-A.SCXP-1-filtered | Lane1_filtered_feature_bc_matrix.h5.txt | https://singlecell.broadinstitute.org/single_cell/study/SCP2670/fc-engineered-antibodies-leverage-neutrophils-to-drive-control-of-mycobacterium-tuberculosis#study-download | Eddie Irvine | H5 | 10x, Single Cell 3' v3 | Lane1_filtered_barcodes.tsv | TSV | Lane1_filtered_features.tsv | TSV | Lane1_metrics_summary.csv | CSV | GRCh38-2020-A | /mnt/enterprise/references/refdata-gex-GRCh38-2020-A | cellranger-6.1.2 | https://fairdomhub.org/sops/733 | DNA-240604ALT-1; DNA-240606ALT-1; DNA-240606ALT-2; DNA-240606ALT-3 | Alter | Single Cell Portal | SCP2670 | bc89684f9bc5162fc5a313c3ecde9c46 | 4f4f3f297a644adcf8d146bb7340715f | 283dc7ac5095a6dfbf8267dc1359757d | 5bdecc12045a72a9ff70aa7632ab867c | MD5 |
| A.SCXP-240604ALT-3 | EI-A.SCXP-2-raw | Lane2_raw_feature_bc_matrix.h5.txt | https://singlecell.broadinstitute.org/single_cell/study/SCP2670/fc-engineered-antibodies-leverage-neutrophils-to-drive-control-of-mycobacterium-tuberculosis#study-download | Eddie Irvine | H5 | 10x, Single Cell 3' v3 | Lane2_raw_barcodes.tsv | TSV | Lane2_raw_features.tsv | TSV | Lane2_metrics_summary.csv | CSV | GRCh38-2020-A | /mnt/enterprise/references/refdata-gex-GRCh38-2020-A | cellranger-6.1.2 | https://fairdomhub.org/sops/733 | DNA-240604ALT-1; DNA-240606ALT-1; DNA-240606ALT-2; DNA-240606ALT-3 | Alter | Single Cell Portal | SCP2670 | 711d59b7fdfdc766e7c5d9388bf195f0 | cfa898666d3cfc8cec500602998332cc | 283dc7ac5095a6dfbf8267dc1359757d | 862c2a0b7db319f4b53ce7a5cd519885 | MD5 |
| A.SCXP-240604ALT-4 | EI-A.SCXP-2-filtered | Lane2_filtered_feature_bc_matrix.h5.txt | https://singlecell.broadinstitute.org/single_cell/study/SCP2670/fc-engineered-antibodies-leverage-neutrophils-to-drive-control-of-mycobacterium-tuberculosis#study-download | Eddie Irvine | H5 | 10x, Single Cell 3' v3 | Lane2_filtered_barcodes.tsv | TSV | Lane2_filtered_features.tsv | TSV | Lane2_metrics_summary.csv | CSV | GRCh38-2020-A | /mnt/enterprise/references/refdata-gex-GRCh38-2020-A | cellranger-6.1.2 | https://fairdomhub.org/sops/733 | DNA-240604ALT-1; DNA-240606ALT-1; DNA-240606ALT-2; DNA-240606ALT-3 | Alter | Single Cell Portal | SCP2670 | 63f85c9c5da3497ba7d2c34b45256585 | b7efb4df1174e657180040d483ec0f61 | 283dc7ac5095a6dfbf8267dc1359757d | 862c2a0b7db319f4b53ce7a5cd519885 | MD5 |
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| UID | Name | File_PrimaryData | Link_PrimaryData | DataType | Protocol | Parent | Scientist | Lab | Checksum_PrimaryData | Software | Checksum_PrimaryType |
| A.SCCL-240604ALT-1 | EI-SCCL | scObject_FcEngineering_EI.rds | https://singlecell.broadinstitute.org/single_cell/study/SCP2670/fc-engineered-antibodies-leverage-neutrophils-to-drive-control-of-mycobacterium-tuberculosis#study-download | RDS | https://fairdomhub.org/sops/733 | A.SCXP-240604ALT-1; A.SCXP-240604ALT-2; A.SCXP-240604ALT-3; A.SCXP-240604ALT-4 | Eddie Irvine | Alter | eff39973b86938f029de991e0657fed1 | Seurat FindClusters | MD5 |
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| UID | Name | File_PrimaryData | Link_PrimaryData | Checksum_PrimaryData | Checksum_PrimaryType | Parent | Scientist | SampleCreationDate | Protocol | Type | LightEmission_Plasmid | LightEmission_Reporter | BacterialStrain | MultiplicityOfInfection | InfectedCells | Cells_Vendor | Cells_Catalog# | NumberInfectedCells | ExcitationWavelength | EmissionWavelength | ReadingIntervals | Analyzer | Analyzer_Vendor | Dilution | |||||||||||||||||||||||||||||||
| D.BSRA-240502ALT-1 | BSRA-Macrophage | MRA_MBL-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | ea17dee57c0ec75bd0a7890fe1c1d0e9 | MD5 | BAC-220920ALT-1; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2018-04-15T0:0:0+0000 | https://fairdomhub.org/sops/729 | Macrophage Restriction Assay | mCherry for constitutive expression, GFP under anhydrotetracycline inducible promoter | GFP, mCherry | H37Rv Mtb Live/dead Reporter | 1.0x10^6 bacteria per mL of blood | Human monocyte-derived macrophages (MDMs) | N/A | N/A | N/A | N/A | N/A | Fixed and analyzed 3 days post-infection, with additional 16hr post-anhydrotetracycline addition | Columbus Image Data Storage and Analysis System | Revvity | Anhydrotetracycline added at 200 ng/mL to induce GFP expression; monoclonal antibody at 50μg/mL in R10 media without phenol | |||||||||||||||||||||||||||||||
| D.BSRA-240502ALT-2 | BSRA-WBA-1 | WBA_180801-BSRA-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | 7ec236e5c5759de59b3d158cba4acf1f | MD5 | BAC-240502ALT-3; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2018-08-01T0:0:0+0000 | https://fairdomhub.org/sops/729 | Whole Blood Restriction Assay | https://www.addgene.org/26161/ | Photorhabdus luminescens | H37Rv Mtb-Lux | 1.0x10^6 bacteria per mL of blood | Human whole blood cells | N/A | N/A | N/A | N/A | N/A | Immediately after infection, then every 24 hours up to 120 hours | Tecan Spark 10M | Tecan | Antibody treatments at 25ug/mL in 300µL total volume; Rifampin at 0.25ug/mL | |||||||||||||||||||||||||||||||
| D.BSRA-240502ALT-3 | BSRA-WBA-2 | WBA_180919-BSRA-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | fc1fb6a448d4081b51af26b2b612b0e4 | MD5 | BAC-240502ALT-3; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2018-09-19T0:0:0+0000 | https://fairdomhub.org/sops/729 | Whole Blood Restriction Assay | https://www.addgene.org/26161/ | Photorhabdus luminescens | H37Rv Mtb-Lux | 1.0x10^6 bacteria per mL of blood | Human whole blood cells | N/A | N/A | N/A | N/A | N/A | Immediately after infection, then every 24 hours up to 120 hours | Tecan Spark 10M | Tecan | Antibody treatments at 25ug/mL in 300µL total volume; Rifampin at 0.25ug/mL | |||||||||||||||||||||||||||||||
| D.BSRA-240502ALT-4 | BSRA-Monocyte-Depletion | Monocyte_Depletion-BSRA-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | ec3fabbf91fab5fd1f62c4a295bd3aec | MD5 | BAC-240502ALT-3; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2021-09-23T0:0:0+0000 | https://fairdomhub.org/sops/729 | Blood Immune Cell Depletion (Monocyte) before Whole Blood Restriction | https://www.addgene.org/26161/ | Photorhabdus luminescens | H37Rv Mtb-Lux | 1.0x10^6 bacteria per mL of blood | Human whole blood cells | N/A | N/A | N/A | N/A | N/A | Immediately after infection, then every 24 hours up to 120 hours | Tecan Spark 10M | Tecan | 100uL of microbeads per mL of blood, with microbeads buffer-exchanged into 2mM EDTA-PBS | |||||||||||||||||||||||||||||||
| D.BSRA-240502ALT-5 | BSRA-Neutrophil-Depletion | Neutrophil_Depletion-BSRA-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | 0e3342ec975c1c5f4f1e004f1a7ff553 | MD5 | BAC-240502ALT-3; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2021-09-23T0:0:0+0000 | https://fairdomhub.org/sops/729 | Blood Immune Cell Depletion (Neutrophil) before Whole Blood Restriction | https://www.addgene.org/26161/ | Photorhabdus luminescens | H37Rv Mtb-Lux | 1.0x10^6 bacteria per mL of blood | Human whole blood cells | N/A | N/A | N/A | N/A | N/A | Immediately after infection, then every 24 hours up to 120 hours | Tecan Spark 10M | Tecan | 100uL of microbeads per mL of blood, with microbeads buffer-exchanged into 2mM EDTA-PBS | |||||||||||||||||||||||||||||||
| D.BSRA-240502ALT-6 | BSRA-Complement-Inhibition | 231016_Complement_Inhibition-BSRA-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | 44e66cde81ec19bfb8b4c805b811c8ed | MD5 | BAC-240502ALT-3; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2023-10-16T0:0:0+0000 | https://fairdomhub.org/sops/729 | Complement Inhibition before Whole Blood Restriction | https://www.addgene.org/26161/ | Photorhabdus luminescens | H37Rv Mtb-Lux | 1.0x10^6 bacteria per mL of blood | Human whole blood cells | N/A | N/A | N/A | N/A | N/A | Immediately after infection, then every 24 hours up to 120 hours | Tecan Spark 10M | Tecan | Cobra venom factor at 50ug/mL | |||||||||||||||||||||||||||||||
| D.BSRA-240502ALT-7 | BSRA-Cytokine-Blockade | 231016_Cytokine_Blockade-BSRA-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2542 | 05144ee484c2f320a5082077a54c9b7b | MD5 | BAC-240502ALT-3; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2023-09-26T0:0:0+0000 | https://fairdomhub.org/sops/729 | Blood Cytokine Blockade before Whole Blood Restriction | https://www.addgene.org/26161/ | Photorhabdus luminescens | H37Rv Mtb-Lux | 1.0x10^6 bacteria per mL of blood | Human whole blood cells | N/A | N/A | N/A | N/A | N/A | Immediately after infection, then every 24 hours up to 120 hours | Tecan Spark 10M | Tecan | Anti-IL-1B and Anti-IFNy at 1ug/mL | |||||||||||||||||||||||||||||||
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| UID | Name | File_PrimaryData | Link_PrimaryData | Checksum_PrimaryData | Checksum_PrimaryType | Parent | Scientist | SampleCreationDate | Protocol | Type | Procedure | PurificationKit | PurificationKit_Vendor | PurificationKit_Catalog# | Filter | Filter_Vendor | Filter_Catalog# | Analyte | AnalyteVolume | AnalyteVolumeUnits | DigestiveEnzyme | DigestiveEnzyme_Vendor | DigestiveEnzyme_Catalog# | DigestiveTemperature | DigestiveTemperatureUnits | DomainSelectionTarget | DomainBeadSelector | Bead_Vendor | Bead_Catalog# | SelectionTemperature | SelectionTemperatureUnits | IsolationTarget | IsolationKit | IsolationKit_Vendor | IsolationKit_Catalog# | CaptureKit | CaptureKit_Vendor | CaptureKit_Catalog# | Label | LabellingKit | LabellingKit_Vendor | LabellingKit_Catalog# | AnalysisMethod | Ladder | Ladder_Vendor | Ladder_Catalog# | Analyzer | AnalyzerSoftware |
| D.GLYN-240506ALT-1 | Irvine Fc-engineering GLYN | Glycans-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | 9e0a7cdf2d1fb2b8e01cd6aef40f5d6e | MD5 | AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2018-02-22T0:0:0+0000 | https://fairdomhub.org/sops/731 | Total IgG | Purification from cell supernatant | Melon Gel IgG Purification Kit | ThermoFisher | 45206 | 0.2 uM; 300 kDa | Fisher; Amicon | 09-719C; UFC5010 | Whole IgG | 20 | ug | IdeS | Promega | V7511 | 37 | Celsius | Fc | Protein G Beads | Millipore | LSKMAGG02 | RT | N/A | Glycans | Glycan Assure APTS Kit | Life Technologies | A28676 | Glycan Assure APTS Kit | Life Technologies | A28676 | APTS | Glycan Assure APTS Kit | Life Technologies | A28676 | Capillary Electrophoresis | LIZ 600 DNA ladder in Hi-Di formamide | ThermoFisher | 4366589 | Applied Biosystems 3500/3500xL Genetic Analyzer | GlycanAssure Data Acquisition Software v1.0 |
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| UID | Name | File_PrimaryData | Link_PrimaryData | Checksum_PrimaryData | Checksum_PrimaryType | Parent | Scientist | SampleCreationDate | Protocol | Type | Analyte | AnalyteVolume | AnalyteVolumeUnits | Analyte_Vendor | Analyte_Catalog# | AnalyteProcedure | Plate | Plate_Vendor | Plate_Catalog# | PlateWash | PlateBlocker | PlateBlockerProcedure | NegativeControl | NegativeControl_Vendor | NegativeControl_Catalog# | Detector | Detector_Vendor | Detector_Catalog# | ReactionStopper | Instrument | Absorbance | AbsorbanceUnits |
| D.ELSA-230208ALT-1 | Irvine Fc-engineering ELSA | Glucan-ELISA-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | b459aa49b9ae0c6e015eb188982176d8 | MD5 | CHM-220929ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2029-02-13T0:0:0+0000 | https://fairdomhub.org/sops/730 | Indirect ELISA | Bovine Liver Glycogen | 50 | uL | Millipore Sigma | G0885 | 100ug/mL overnight at 4C | NUNC MaxiSorp flat bottom | ThermoFisher | 63494 | PBS | 5% (BSA) Bovine Serum Albumin-PBS | 2 hours at RT on orbital shaker | Human IgG1 Isotype Control Antibody | Immune Technology | IT-001-37G12 | TMB | ThermoFisher | 34029 | 2N H2SO4 | Tecan Infinite M1000 Pro | 450 | nm |
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| UID | Name | File_PrimaryData | Link_PrimaryData | Checksum_PrimaryData | Checksum_PrimaryType | Parent | Scientist | SampleCreationDate | Protocol | ELISAplate | ELISAplate_Vendor | ELISAplate_Catalog# | Antigen | Blocking_solution | NKC_Name | AntiCD107a_Name | AntiCD107a_Ab_Vendor | AntiCD107a_Ab_Fluorophore | AntiCD107a_Ab_Catalog# | CD3_Name | CD3_Ab_Vendor | CD3_Ab_Fluorophore | CD3_Ab_Catalog# | CD56_Name | CD56_Ab_Vendor | CD56_Ab_Fluorophore | CD56_Ab_Catalog# | CD16_Name | CD16_Ab_Vendor | CD16_Ab_Fluorophore | CD16_Ab_Catalog# | MIP-1B_Name | MIP-1B_Ab_Vendor | MIP-1B_Ab_Fluorophore | MIP-1B_Ab_Catalog# | INFg_Name | INFg_Ab_Vendor | INFg_Ab_Fluorophore | INFg_Ab_Catalog# | Fixed | Fixative | Analyzer | Analyzer_Vendor | Software | TNFa_Name | TNFa_Ab_Vendor | TNFa_Ab_Fluorophore | TNFa_Ab_Catalog# | ||
| D.ADNKA-240502ALT-1 | Irvine Fc-engineering ADNKA | ADNKA-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | e6f703eec913dc0284821c19d7ae9238 | MD5 | CHM-220929ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2; TIS-240502ALT-1 | Eddie Irvine | 2017-11-17T0:0:0+0000 | https://fairdomhub.org/sops/727 | NUNC MaxiSorp flat bottom | ThermoFisher | 442404 | Bovine liver glycogen | 5% BSA-PBS | NK cells enriched from healthy donor blood using RosetteSep Kit (Stemcell, 15065) | BD Pharmingen PE-Cy5 Mouse Anti-Human IgM | BD Biosciences | PE-Cy5 | 551079 | Anti-human CD3 | BD Biosciences | BD Pharmingen Alexa Fluor 700 Mouse Anti-Human CD3 | 557943 | BD Pharmingen PE-Cy7 Mouse Anti-Human CD56 (NCAM-1) | BD Biosciences | PE-Cy7 | 557747 | BD Pharmingen APC-Cy7 Mouse Anti-Human CD16 | BD Biosciences | APC-Cy7 | 557758 | BD Pharmingen PE Mouse Anti-Human MIP-1B | BD Biosciences | PE | 550078 | BD Pharmingen FITC Mouse Anti-Human IFN-g | BD Biosciences | FITC | 554551 | Yes | Perm A and Perm B (Invitrogen) | BD LSRII | BD Biosciences | FlowJo 10.3 | NA | NA | NA | NA | ||
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| UID | Name | File_PrimaryData | Link_PrimaryData | Checksum_PrimaryData | Checksum_PrimaryType | Parent | Scientist | SampleCreationDate | Protocol | Bead_Fluorophore | Bead_Vendor | Bead_Catalog# | Bead_Coating | PhagocyticCells | PhagocyticCells_Vendor | PhagocyticCells_Catalog# | Analyzer | Analyzer_Vendor | Fixed | Fixative | Software |
| D.ADCP-240502ALT-1 | Irvine Fc-engineering ADCP Replicate 1 | ADCP-FcEngineered-Replicate-1-EI.xlsx | https://immport.org/shared/study/SDY2541 | 97e9efe60715c2488cf14adc42e2d047 | MD5 | AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2; BAC-240502ALT-2 | Eddie Irvine | 2017-12-06T0:0:0+0000 | https://fairdomhub.org/sops/726 | FluoSpheres NeutrAvidin-Labeled Microspheres, Red fluorescent (580/605) | ThermoFisher | F8775 | H37Rv whole-cell lysate (Vendor: BEI, Product#: NR-14822) | THP-1 monocytes | ATCC | TIB-202 | BD LSRII | BD BioSciences | Yes | 4% PFA | FlowJo 10.3 |
| D.ADCP-240502ALT-2 | Irvine Fc-engineering ADCP Replicate 2 | ADCP-FcEngineered-Replicate-2-EI.xlsx | https://immport.org/shared/study/SDY2541 | 4b75ae4a061db420e534d4c9e40cbd55 | MD5 | AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2; BAC-240502ALT-2 | Eddie Irvine | 2018-02-14T0:0:0+0000 | https://fairdomhub.org/sops/726 | FluoSpheres NeutrAvidin-Labeled Microspheres, Red fluorescent (580/605) | ThermoFisher | F8775 | H37Rv whole-cell lysate (Vendor: BEI, Product#: NR-14822) | THP-1 monocytes | ATCC | TIB-202 | BD LSRII | BD BioSciences | Yes | 4% PFA | FlowJo 10.3 |
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| UID | Name | File_PrimaryData | Link_PrimaryData | Checksum_PrimaryData | Checksum_PrimaryType | Parent | Scientist | SampleCreationDate | Protocol | Bead_Fluorophore | Bead_Vendor | Bead_Catalog# | Bead_coating | Leukocytes | Analyzer | Analyzer_Vendor | Fixed | Fixative | Staining_Antibody | Staining_Antibody_Vendor | Staining_Antibody_Fluorophore |
| D.ADNP-240502ALT-1 | Irvine Fc-engineering ADNP Replicate 1 | ADNP-FcEngineered-Replicate-1-EI.xlsx | https://immport.org/shared/study/SDY2541 | e15acf9207d29b96cb742485718ac080 | MD5 | AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2; TIS-240502ALT-1; BAC-240502ALT-2 | Eddie Irvine | 2017-12-05T0:0:0+0000 | https://fairdomhub.org/sops/728 | FluoSpheres NeutrAvidin-Labeled Microspheres, Red fluorescent | ThermoFisher | F8775 | H37Rv whole-cell lysate | Neutrophils from healthy blood donors | BD LSRII | BD BioSciences | Yes | 4% PFA | CD66b antibody | BioLegend | Pacific Blue |
| D.ADNP-240502ALT-2 | Irvine Fc-engineering ADNP Replicate 2 | ADNP-FcEngineered-Replicate-2-EI.xlsx | https://immport.org/shared/study/SDY2541 | 2e0eb81952c7bb5241e7c5fa19d38b3c | MD5 | AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2; TIS-240502ALT-1; BAC-240502ALT-2 | Eddie Irvine | 2018-02-02T0:0:0+0000 | https://fairdomhub.org/sops/728 | FluoSpheres NeutrAvidin-Labeled Microspheres, Red fluorescent | ThermoFisher | F8775 | H37Rv whole-cell lysate | Neutrophils from healthy blood donors | BD LSRII | BD BioSciences | Yes | 4% PFA | CD66b antibody | BioLegend | Pacific Blue |
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| UID | Name | File_PrimaryData | Link_PrimaryData | Checksum_PrimaryData | Checksum_PrimaryType | Parent | Scientist | SampleCreationDate | Protocol | Bead_Fluorophore | Bead_Vendor | Bead_Catalog# | Bead_coating | Complement | Complement_Vendor | Complement_Catalog# | CD3Ab_Name | C3Ab_Vendor | C3Ab_Catalog# | C3Ab_Fluorophore | Analyzer | Analyzer_Vendor |
| D.ADCD-240502ALT-1 | Irvine Fc-engineering ADCD Replicate 1 | ADCD-FcEngineered-Replicate-1-EI.xlsx | https://immport.org/shared/study/SDY2541 | 1672cf74fccc5269c29d0097f2fa17f5 | MD5 | AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2; BAC-240502ALT-2 | Eddie Irvine | 2017-12-05T0:0:0+0000 | https://fairdomhub.org/sops/725 | FluoSpheres NeutrAvidin-Labeled Microspheres, Red fluorescent (580/605) | ThermoFisher | F8775 | H37Rv whole-cell lysate (Vendor: BEI, Product#: NR-14822) | Guinea pig | Cederlane | CL4501 | FITC IgG Goat Anti-Guinea Pig Complement C3 | MP Biomedicals | MP0855385 | Fluorescein Isothiocyanate | BD LSRII | BD Biosciences |
| D.ADCD-240502ALT-2 | Irvine Fc-engineering ADCD Replicate 2 | ADCD-FcEngineered-Replicate-2-EI.xlsx | https://immport.org/shared/study/SDY2541 | da11b8abe39fb5fecea2088e1f9d3e20 | MD5 | AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2; BAC-240502ALT-2 | Eddie Irvine | 2018-02-14T0:0:0+0000 | https://fairdomhub.org/sops/725 | FluoSpheres NeutrAvidin-Labeled Microspheres, Red fluorescent (580/605) | ThermoFisher | F8775 | H37Rv whole-cell lysate (Vendor: BEI, Product#: NR-14822) | Guinea pig | Cederlane | CL4501 | FITC IgG Goat Anti-Guinea Pig Complement C3 | MP Biomedicals | MP0855385 | Fluorescein Isothiocyanate | BD LSRII | BD Biosciences |
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| UID | Name | File_PrimaryData | Link_PrimaryData | Checksum_PrimaryData | Checksum_PrimaryType | Parent | Scientist | SampleCreationDate | Protocol | Panel | Analyte | Vendor | Product# | Lot# | BeadFluorescence | MeasurementWavelength | MeasurementWavelengthUnits | Instrument | Software | LuminexDesign | Notes |
| D.LMX-240502ALT-1 | Irvine Fc-engineering LMX | CytokineLuminex-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | 2a46281a5ec58cc247d058d864138532 | MD5 | TIS-240502ALT-2 | Eddie Irvine | 2021-12-15T0:0:0+0000 | https://fairdomhub.org/sops/732 | Human Cytokine Magnetic 10-Plex Panel (ThermoFisher, LHC0001M) | GM-CSF; IFN-y; IL-1B; IL-2; IL-4; IL-5; IL-6; IL-8; IL-10; TNF-a | ThermoFisher | LHC0001M | NA | Streptavidin R-Phycoerythrin | 565 | nm | FlexMap 3D | N/A | magnetic bead-based multiplex panel measuring median fluorescence intensity | The assay was conducted at the final timepoint of the whole-blood restriction assay (120hr) to measure cytokine levels in the supernatant. Supernatants were collected, stored at -20°C, thawed, and filtered before analysis. |
