HSD11B1 inhibition in human adipose tissue

Selected Cell

Cell:

Value:

Subcutaneous Omental

	Adipose tissue incubated with either 100 nM cortisone or 100 nM 11KA4 with or without inhibitor (AZD)
	NB: Concentrations normalised to tissue mass so all data presented as nMol/L/mg tissue

	Control		AZD			Control				AZD
	Cortisone	Cortisone	Cortisone	Cortisol		11KA4	11OHA4	11KT	11OHT	11KA4	11OHA4	11KT	11OHT
	0.07277822247329019	0.24001502119264678	0.3731463407938981	0.01839633735802051		0.006264683694363619	0.043207	0.047513	0.224392	0.0023592644978783594	0.002178	0.241021	0.036775
	0.013578879736430804	0.2212731634763451	0.30993856399320846	0.023951252367392557		0.0010105509964830012	0.049143	0.016164	0.167873	0.0015587061028273451	0.003105	0.188261	0.051107
	0.21807029529335217	0.1633179579484479	0.38014767861286003	0.008201835950247075		0.044726405622489945	0.053347	0.114618	0.154176	0.015023245756297973	0.002666	0.281935	0.021618
	0.08119314056174595	0.23072201851498514	0.4197983951625217	0.015741674523889155		0.11041200324412002	0.099068	0.06255	0.193092	0.0336538973131152	0.003573	0.303929	0.05218
	0.05688906533930477	0.3317623143730931	0.3066689610920552	0.009987150370896215		0.0027064708386547462	0.115858	0.012608	0.187352	0.009402013025458848	0.004264	0.210021	0.028437

Ave	0.08850192068082477	0.23741809510110362	0.35793998793090875	0.015255650114089104	Ave	0.033024022879222265	0.07212460000000001	0.050690599999999995	0.185377	0.012399425339115543	0.0031572	0.2450334	0.0380234
SD	0.07697637842072418	0.06062982417924332	0.04869222764263338	0.006388016484809914	SD	0.04686662950372445	0.032998245670035224	0.04147235679099996	0.026765110180980132	0.013095299885179825	8.063390725991146E-4	0.048206567735112615	0.013547920774052386


	qPCR data (Arbitrary units)
	AKR1C3	HSD11B1	HSD11B1/AKR1C3
	0.396060965	0.074604242	0.18836555124789944
	0.435460663	0.085642831	0.1966717967358627
	0.087780048	0.009212501	0.10494982869000026
	0.444776906	0.043030603	0.09674648665324363
	0.206700637	0.051134073	0.2473822710086762
	0.312248894	0.027127765	0.08687865840767398
	0.243243065	0.035992861	0.1479707592074619
	0.18658672	0.02305702	0.12357267441112636
	0.292713367	0.02809487	0.09598082345176946
	0.318578212	0.046281012	0.14527362593145574

Ave	0.29241494770000004	0.0424177778	0.14337924757451695
SD	0.11466695922636307	0.023459863412923703	0.05290224212758273

	Adipose tissue incubated with either 100 nM cortisone or 100 nM 11KA4 with or without inhibitor (AZD)
	NB: Concentrations normalised to tissue mass so all data presented as nMol/L/mg tissue

	Control		AZD			Control				AZD
	Cortisone	Cortisone	Cortisone	Cortisol		11KA4	11OHA4	11KT	11OHT	11KA4	11OHA4	11KT	11OHT
	0.05623196965200824	0.1707827863071292	0.19939205450664496	0.006623602683301466		0.006264683694363619	0.033015161344886884	0.03630607888352894	0.0495953260397229	0.0023592644978783594	0.00176475547270905	0.19531271954063906	0.029800677436164675
	0.1270592655700169	0.15234839019975116	0.23623901868400177	0.006951642794597449		0.0010105509964830012	0.03857829398383578	0.012689145439159027	0.07989331949734878	0.0015587061028273451	0.002460578138734519	0.14920735389103204	0.04050531333221835
	0.2366773043368463	0.13124028212378658	0.3210406059754278	0.013967769233952496		0.044726405622489945	0.04388913268969918	0.0942973051144977	0.13427357548968938	0.015023245756297973	0.002208221543907735	0.2334861330990498	0.017902717790258964
	0.28684749496546735	0.16977350960074256	0.3589120156842761	0.006718697295614346		0.11041200324412002	0.08292325292857025	0.052356552424227765	0.053177509786157484	0.0336538973131152	0.0030549198479939543	0.2598677082287279	0.04461529172092243
	0.11021005823460721	0.18014998822300016	0.32182161135280046	0.007993692077278628		0.0027064708386547462	0.0904643796571177	0.009844967141153484	0.13955607909909304	0.009402013025458848	0.0036372588394043923	0.1791667156787785	0.024259138076096094

Ave	0.1634052185517892	0.16085899129088194	0.2874810612406302	0.008451080816948878	Ave	0.033024022879222265	0.05777404412082197	0.041098809800513385	0.09129916198240232	0.012399425339115543	0.00262514676854993	0.20340812608764552	0.031416627671132104
SD	0.09518882920351261	0.019363568695579023	0.0666957976755263	0.0031317806008045565	SD	0.04686662950372445	0.026811427629909717	0.03450977634090541	0.04329576713491079	0.013095299885179825	7.331879708855572E-4	0.04382714972994841	0.011104904912678935


	qPCR data (Arbitrary units)
	AKR1C3	HSD11B1	HSD11B1/AKR1C3
	0.031207515	0.010198656	0.3268012848828239
	0.207224923	0.057840285	0.2791183809488012
	0.280051291	0.049344447	0.17619789154980187
	0.174831897	0.047256135	0.2702946991417704
	0.32857841	0.076232066	0.2320057060352809
	0.138271639	0.015750013	0.11390631595825663
	0.132371513	0.029334909	0.2216104381914861
	0.058510498	0.012091865	0.2066614609911541
	0.232168228	0.04564469	0.19660179342024353
	0.182059701	0.039941995	0.21938954519100304

Ave	0.17652756149999999	0.0383635061	0.22425875163106218
SD	0.09211558568433623	0.021460256460710973	0.05896955416122213