Kinetic data for PFK-1 and PFK-2

Selected Cell

Cell:

Value:

PFK1-Kinetics PFK1-Effectors PFK1-kin-with 3-PG PFK1-more 3PG PFK1-ADP Sat 2 mM 3-PG PFK1-3-PG Sat 0.4 mM F6P PFK2-F6P PFK2-ADP PFK2-ATP_inhibitin

Note	PFK-1 [µg]	FBPA [U]	TPI [U]	GPDH [U]	NADH [mM]	F6P [mM]	ADP [mM]	MgCl2 [mM]	Buffer [mM]	spez. A1 [U/mg]	spez. A2 [U/mg]	spez. A3 [U/mg]	Mean [U/mg]	SD
Buffer: HEPES/NaOH, pH7.7 at 30°C	2	0.5	5	0.5	0.2	3	1	10	100	7.851688102893891	8.032556270096464	7.463826366559487	7.782690246516615	0.2905752121788597
30°C	2	0.5	5	0.5	0.2	3	0.75	10	100	6.682073954983923	7.088022508038585	6.57556270096463	6.781886387995712	0.2704175563907945
PFK: phosphofructokinase	2	0.5	5	0.5	0.2	3	0.5	10	100	6.340434083601286	6.953376205787782	6.461012861736335	6.584941050375135	0.32472012293988145
FBPA: fructose 1,6-bisphosphate aldolase	2	0.5	5	0.5	0.2	3	0.25	10	100	5.046221864951768	5.389871382636656	6.189710610932476	5.541934619506967	0.5867146231787138
TPI: triose phosphate isomerase	2	0.5	5	0.5	0.2	3	0.15	10	100	4.320739549839228	4.604099678456592	4.411173633440515	4.445337620578779	0.14473639533786872
GPDH: glycerol 3-phosphate dehydrogenase	2	0.5	5	0.5	0.2	3	0.1	10	100	3.703778135048231	3.5811897106109325	3.6495176848874604	3.644828510182208	0.06142859042064289
	2	0.5	5	0.5	0.2	3	0.05	10	100	2.2628617363344055	2.5462218649517685	2.4678456591639875	2.4256430868167205	0.1463182608462008
	2	0.5	5	0.5	0.2	3	0.025	10	100	1.786575562700965	2.1824758842443734	1.8488745980707395	1.939308681672026	0.21288026859515868
	2	0.5	5	0.5	0.2	3	0.01	10	100	0.4863344051446945	0.4742765273311897	0.5546623794212219	0.505091103965702	0.04335125854063088
	2	0.5	5	0.5	0.2	3	1.0E-7	10	100	0.012057877813504824	0.004019292604501608	0.02009646302250804	0.012057877813504822	0.008038585209003217








Note	PFK-1 [µg]	FBPA [U]	TPI [U]	GPDH [U]	NADH [mM]	F6P [mM]	ADP [mM]	MgCl2 [mM]	Buffer [mM]	spez. A1 [U/mg]	spez. A2 [U/mg]	spez. A3 [U/mg]	Mean [U/mg]	SD
Buffer: HEPES/NaOH, pH7.7 at 30°C	2.32	0.5	5	0.5	0.2	1	3	10	100	6.9869858077392175	7.5898796984144585	7.304024836456369	7.293630114203348	0.30158132989530445
30°C	2.32	0.5	5	0.5	0.2	0.75	3	10	100	6.945406918727131	7.57255516132609	7.479002661048897	7.332321580367373	0.33832711112348873
PFK: phosphofructokinase	2.32	0.5	5	0.5	0.2	0.5	3	10	100	7.103060206231289	6.754837010755073	7.893059097460917	7.250318771482426	0.5832248223850894
FBPA: fructose 1,6-bisphosphate aldolase	2.32	0.5	5	0.5	0.2	0.25	3	10	100	6.184859740547733	6.595451269542079	6.238565805521676	6.339625605203829	0.22317294471849952
TPI: triose phosphate isomerase	2.32	0.5	5	0.5	0.2	0.15	3	10	100	5.277053997117197	4.897646634881916	5.233742654396274	5.136147762131795	0.20768018412133088
GPDH: glycerol 3-phosphate dehydrogenase	2.32	0.5	5	0.5	0.2	0.1	3	10	100	3.918810289389068	3.8599068632886135	3.9759812617806847	3.9182328048194552	0.05803935399920589
	2.32	0.5	5	0.5	0.2	0.05	3	10	100	2.6610488967734782	2.8083074620246147	2.853351258454374	2.774235872417489	0.1005768586245355
	2.32	0.5	5	0.5	0.2	0.025	3	10	100	1.261226300033263	0.8107883357356692	1.488177735890897	1.1867307905532765	0.34478440446194347
	2.32	0.5	5	0.5	0.2	0.01	3	10	100	0.6271482425989579	0.6028938906752412	0.6011614369664043	0.6104011900802012	0.014529217976643211
	2.32	0.5	5	0.5	0.2	1.0E-7	3	10	100	0.010394722253021401	0.012127175961858298	0.0051973611265107	0.0092397531137968	0.0036063899813347715

Note	Effector	PFK-1 [µg]	FBPA [U]	TPI [U]	GPDH [U]	NADH [mM]	F6P [mM]	ADP [mM]	Effector [mM]	MgCl2 [mM]	Buffer [mM]	spez. A1 [U/mg]	spez. A2 [U/mg]	spez. A3 [U/mg]	Mean [U/mg]	SD	relative activity in % for A1	relative activity in % for A2	relative activity in % for A3	Mean relative activity in %	Mean stabw
Buffer: HEPES/NaOH, pH7.7 at 30°C	No effector	1.86	0.5	5	0.5	0.2	0.08	0.4	0	10	100	2.7162638730422155	2.8934584932406735	2.6363102029526675	2.7486775230785185	0.13160278998189254	100	100	100	100	0
30°C	Citrate	1.86	0.5	5	0.5	0.2	0.08	0.4	1	10	100	2.5044946928050345	3.1657331535456215	2.973412163330222	2.8812133365602928	0.34012430556189127	92.20365950676215	109.4100074682599	112.78688524590166	104.80018407364123	11.038801984557653
PFK: phosphofructokinase	PEP	1.86	0.5	5	0.5	0.2	0.08	0.4	1	10	100	2.405092832693704	2.3424264426235175	2.2905646025654325	2.346027959294218	0.057348993590346294	88.54415274463008	80.95593726661687	86.88524590163935	85.46177863762877	3.9893543116614936
FBPA: fructose 1,6-bisphosphate aldolase	ATP	1.86	0.5	5	0.5	0.2	0.08	0.4	1	10	100	2.36619645265014	2.8545621131971095	2.6838502230059125	2.6348695962843873	0.24783982921615827	87.11217183770883	98.65571321882001	101.80327868852459	95.85705458168447	7.7350838877759704
TPI: triose phosphate isomerase	3-PG	1.86	0.5	5	0.5	0.2	0.08	0.4	1	10	100	0.09291913010406944	0.09724095010890986	0.11668914013069184	0.10228307344789038	0.012661777126081169	3.4208432776451874	3.3607169529499625	4.426229508196721	3.7359299129306236	0.5985724201370649
GPDH: glycerol 3-phosphate dehydrogenase	sn-Glycerol 3P	1.86	0.5	5	0.5	0.2	0.08	0.4	1	10	100	2.623344742938146	2.718424783044636	2.7205856930470564	2.6874517396766127	0.055528800240316596	96.57915672235481	93.95070948469008	103.19672131147541	97.90886250617343	4.7642703301664255
	AMP	1.86	0.5	5	0.5	0.2	0.08	0.4	1	10	100	1.6682225218684092	1.8886353421152717	1.903761712132213	1.8202065253719646	0.13183912453516564	61.41607000795545	65.27259148618373	72.21311475409836	66.30059208274584	5.47143790355443
	2-PG	1.86	0.5	5	0.5	0.2	0.08	0.4	1	10	100	2.3618746326453	2.7551602530857795	2.742194793071259	2.6197432262674463	0.22341482598709672	86.95306284805093	95.22031366691562	104.01639344262297	95.39658998586317	8.533030982621638
	D,L-Malate	1.86	0.5	5	0.5	0.2	0.08	0.4	1	10	100	2.294886422570273	2.6968156830204335	2.4375064827300075	2.4764028627735715	0.20376819890962689	84.48687350835323	93.20388349514563	92.45901639344262	90.04992446564715	4.832117415262855
	Na2HPO4	1.86	0.5	5	0.5	0.2	0.08	0.4	1	10	100	2.5390692528437575	2.718424783044636	2.6449538429623485	2.6341492929502475	0.09016460103129473	93.47653142402545	93.95070948469008	100.327868852459	95.91836992039151	3.8260909336073268



















Note	Effector	PFK-1 [µg]	FBPA [U]	TPI [U]	GPDH [U]	NADH [mM]	F6P [mM]	ADP [mM]	3-PG [mM]	MgCl2 [mM]	Buffer [mM]	spez. A1 [U/mg]	spez. A2 [U/mg]	spez. A3 [U/mg]	Mean [U/mg]	SD	relative activity in % for A1	relative activity in % for A2	relative activity in % for A3	Mean relative activity in %	Mean stabw
Buffer: HEPES/NaOH, pH7.7 at 30°C	3-PG	1.89	0.5	5	0.5	0.2	0.1	0.15	1.0E-7	10	100	1.4652341822759831	1.4907535003998025	1.799111927729291	1.585033203468359	0.18583617526000515	100	100	100	100	0
30°C	3-PG	1.89	0.5	5	0.5	0.2	0.1	0.15	0.025	10	100	1.3227513227513228	0.9846203576107114	0.8272512291804895	1.0448743031808412	0.25318568117046514	90.27576197387516	66.0485021398003	45.98108747044917	67.43511719470821	22.1798686498362
PFK: phosphofructokinase	3-PG	1.89	0.5	5	0.5	0.2	0.1	0.15	0.1	10	100	0.5061331427890914	0.5869443168478539	0.49124687388352983	0.5281081111734917	0.051494413037717575	34.542815674891145	39.372325249643374	27.30496453900709	33.74003515451387	6.073601983425911
FBPA: fructose 1,6-bisphosphate aldolase	3-PG	1.89	0.5	5	0.5	0.2	0.1	0.15	0.15	10	100	0.19777471545960293	0.38491638170094766	0.29772537811123023	0.29347215842392693	0.09364330311975376	13.497822931785194	25.820256776034245	16.548463356973997	18.62218102159781	6.417618146625997
TPI: triose phosphate isomerase	3-PG	1.89	0.5	5	0.5	0.2	0.1	0.15	0.25	10	100	0.10420388233893056	0.11909015124449208	0.09357083312067237	0.10562162223469834	0.012818595445103068	7.111756168359941	7.988587731811698	5.200945626477543	6.767096508883061	1.4254226873145375
GPDH: glycerol 3-phosphate dehydrogenase	3-PG	1.89	0.5	5	0.5	0.2	0.1	0.15	0.5	10	100	0.0574184657785944	0.05103863624763947	0.053165246091291106	0.053874116039174996	0.003248450194057287	3.9187227866473147	3.4236804564907284	2.9550827423167845	3.432495328484942	0.4818804937279998
	3-PG	1.89	0.5	5	0.5	0.2	0.1	0.15	1	10	100	0.036152367342077954	0.03189914765477467	0.0382789771857296	0.03544349739419408	0.0032484501940572862	2.4673439767779386	2.139800285306705	2.127659574468085	2.244934612184243	0.19270779254909579





















Note	Effector	PFK-1 [µg]	FBPA [U]	TPI [U]	GPDH [U]	NADH [mM]	F6P [mM]	ADP [mM]	ATP [mM]	MgCl2 [mM]	Buffer [mM]	spez. A1 [U/mg]	spez. A2 [U/mg]	spez. A3 [U/mg]	Mean [U/mg]	SD	relative activity in % for A1	relative activity in % for A2	relative activity in % for A3	Mean relative activity in %	Mean stabw
Buffer: HEPES/NaOH, pH7.7 at 30°C	AMP	2.1	0.5	5	0.5	0.2	0.1	0.15	0	10	100	2.1742459041494415	3.3455826060327674	2.6737865564232126	2.731205022201807	0.587775533221658	100	100	100	100	0
30°C	AMP	2.1	0.5	5	0.5	0.2	0.1	0.15	0.025	10	100	1.5828357066299188	2.3866942275302407	2.361812892359516	2.1104476088398916	0.45709463979048087	72.79929577464787	71.33867276887872	88.33214030064423	77.49003628139027	9.417896238783243
PFK: phosphofructokinase	AMP	2.1	0.5	5	0.5	0.2	0.1	0.15	0.1	10	100	1.8948093706936153	2.0039044556729446	1.9311743990200585	1.9432960751288728	0.05554849831837959	87.14788732394366	59.897025171624705	72.22619899785255	73.09037049780697	13.645968866195316
FBPA: fructose 1,6-bisphosphate aldolase	AMP	2.1	0.5	5	0.5	0.2	0.1	0.15	0.15	10	100	1.8393048537743075	2.2182667279130306	1.862272240085745	1.9732812739243608	0.21247418598036938	84.59507042253522	66.30434782608697	69.64924838940587	73.51622221267601	9.739237556782816
TPI: triose phosphate isomerase	AMP	2.1	0.5	5	0.5	0.2	0.1	0.15	0.25	10	100	1.7876282345735721	2.3560710457816567	2.3024804777216357	2.1487265860256213	0.31386621642395707	82.21830985915491	70.42334096109839	86.11309949892629	79.58491677305986	8.169651161662642
GPDH: glycerol 3-phosphate dehydrogenase	AMP	2.1	0.5	5	0.5	0.2	0.1	0.15	0.5	10	100	1.6383402235492268	1.7761445414178534	1.542642780584903	1.6523758485173277	0.11738192856741608	75.35211267605632	53.08924485125858	57.69506084466715	62.04547279066068	11.751739616796558
	AMP	2.1	0.5	5	0.5	0.2	0.1	0.15	1	10	100	1.2230133210840608	1.3723013321084063	1.255550451691931	1.2836217016281328	0.0785030181817031	56.25	41.01830663615561	46.957766642806014	48.075357759653876	7.677100892321821

Note	PFK-1 [µg]	FBPA [U]	TPI [U]	GPDH [U]	NADH [mM]	F6P [mM]	ADP [mM]	3-PG [mM]	MgCl2 [mM]	Buffer [mM]	spez. A1 [U/mg]	spez. A2 [U/mg]	spez. A3 [U/mg]	Mean [U/mg]	SD
Buffer: HEPES/NaOH, pH7.7 at 30°C	2.49	0.5	5	0.5	0.2	3	1	1	10	100	7.515592918296983	7.554333087978926	7.528506308190964	7.532810771488958	0.019725528889215267
30°C	2.49	0.5	5	0.5	0.2	3	0.75	1	10	100	7.4235850153023675	7.415514146618628	7.078151835638374	7.305750332519789	0.19714738539310514
PFK: phosphofructokinase	2.49	0.5	5	0.5	0.2	3	0.5	1	10	100	6.897364377122639	6.979687237696769	6.58260049845685	6.819884037758752	0.20957545624668242
FBPA: fructose 1,6-bisphosphate aldolase	2.49	0.5	5	0.5	0.2	3	0.25	1	10	100	5.8158679735017245	6.1887421066904285	5.773899456346287	5.926169845512813	0.2283604242486006
TPI: triose phosphate isomerase	2.49	0.5	5	0.5	0.2	3	0.15	1	10	100	3.8142925399346588	3.5221270935833373	4.253347796330015	3.863255809949337	0.36806110958539756
GPDH: glycerol 3-phosphate dehydrogenase	2.49	0.5	5	0.5	0.2	3	0.1	1	10	100	1.4608272317566084	2.125866811296634	1.0298428440449903	1.5388456290327444	0.552161468170915
3-PG: 3-phosphoglycerate	2.49	0.5	5	0.5	0.2	3	0.05	1	10	100	0.2098425857771924	0.2663386665633596	0.3551182220844794	0.2770998248083438	0.07323322006577328
	2.49	0.5	5	0.5	0.2	3	0.025	1	10	100	0.19208667467296844	0.25503945040612613	0.2211418019344258	0.22275597567117345	0.03150741437767689
	2.49	0.5	5	0.5	0.2	3	0.01	1	10	100	0.03874016968194321	0.035511822208447945	0.114606335309082	0.06295277573315772	0.04476240855338411
	2.49	0.5	5	0.5	0.2	3	1.0E-7	1	10	100	0.014527563630728704	0.016141737367476337	0.011299216157233435	0.013989505718479492	0.002465691111151918








Note	PFK-1 [µg]	FBPA [U]	TPI [U]	GPDH [U]	NADH [mM]	F6P [mM]	ADP [mM]	3-PG [mM]	MgCl2 [mM]	Buffer [mM]	spez. A1 [U/mg]	spez. A2 [U/mg]	spez. A3 [U/mg]	Mean [U/mg]	SD
Buffer: HEPES/NaOH, pH7.7 at 30°C	2.6	0.5	5	0.5	0.2	1	3	1	10	100	7.836074697007175	8.029309918377441	7.8128864704427405	7.892757028609119	0.11882525927116926
30°C	2.6	0.5	5	0.5	0.2	0.75	3	1	10	100	7.04612911204551	7.655206529804601	8.03394756369033	7.578427735180147	0.49836488723673145
PFK: phosphofructokinase	2.6	0.5	5	0.5	0.2	0.5	3	1	10	100	7.06622557506802	7.226997279248083	7.28883255008657	7.1940184681342245	0.11490938960150313
FBPA: fructose 1,6-bisphosphate aldolase	2.6	0.5	5	0.5	0.2	0.25	3	1	10	100	5.775414296314617	6.186618847390552	6.157247093742271	6.039760079149147	0.22940072941229725
TPI: triose phosphate isomerase	2.6	0.5	5	0.5	0.2	0.15	3	1	10	100	4.976193420727183	4.801508780608459	4.824697007172891	4.867466402836178	0.0948714750460017
GPDH: glycerol 3-phosphate dehydrogenase	2.6	0.5	5	0.5	0.2	0.1	3	1	10	100	3.255627009646303	3.506059856542172	3.2448058372495674	3.3354975678126806	0.1478103352120267
3-PG: 3-phosphoglycerate	2.6	0.5	5	0.5	0.2	0.05	3	1	10	100	1.0233737323769476	1.1563195646796935	1.9447192678703933	1.3748041883090114	0.498017115183062
	2.6	0.5	5	0.5	0.2	0.025	3	1	10	100	0.36791986148899336	0.40656690576304727	0.4946821667078902	0.42305631131997695	0.0649699613499799
	2.6	0.5	5	0.5	0.2	0.01	3	1	10	100	0.08347761563195648	0.08038585209003216	0.11439525105119962	0.09275290625772942	0.018806463425359306
	2.6	0.5	5	0.5	0.2	1.0E-7	3	1	10	100	0.03246351719020529	0.010821172396735098	0.00927529062577294	0.017519993404237776	0.012964532963773931





Note	PFK-1 [µg]	FBPA [U]	TPI [U]	GPDH [U]	NADH [mM]	F6P [mM]	ADP [mM]	3-PG [mM]	MgCl2 [mM]	Buffer [mM]	spez. A1 [U/mg]	spez. A2 [U/mg]	spez. A3 [U/mg]	Mean [U/mg]	SD
Buffer: HEPES/NaOH, pH7.7 at 30°C	0.22	0.5	5	0.5	0.2	1	3	2	10	100	8.206664717918738	9.030619701841568	9.220622624963463	8.819302348241257	0.5389980772360039
30°C	0.22	0.5	5	0.5	0.2	0.75	3	2	10	100	7.528865828705058	7.653098509207834	8.564747149956155	7.915570495956349	0.5656245979446207
PFK: phosphofructokinase	0.22	0.5	5	0.5	0.2	0.5	3	2	10	100	7.335209003215434	6.4052908506284725	7.282227418883368	7.007575757575758	0.5222663055587249
FBPA: fructose 1,6-bisphosphate aldolase	0.22	0.5	5	0.5	0.2	0.25	3	2	10	100	2.8043700672318037	3.1661064016369487	3.7964045600701555	3.2556270096463025	0.5020394040202775
TPI: triose phosphate isomerase	0.22	0.5	5	0.5	0.2	0.15	3	2	10	100	1.757527038877521	1.6314674071908801	1.1418444899152296	1.510279645327877	0.3252400736274645
GPDH: glycerol 3-phosphate dehydrogenase	0.22	0.5	5	0.5	0.2	0.1	3	2	10	100	1.0797281496638411	1.0121309558608595	0.984726688102894	1.0255285978758648	0.04889725997794463
3-PG: 3-phosphoglycerate	0.22	0.5	5	0.5	0.2	0.05	3	2	10	100	0.49327681964337916	0.3452937737503654	0.21558023969599532	0.35138361102991333	0.13894841566994184
	0.22	0.5	5	0.5	0.2	0.025	3	2	10	100	0.17904121601870795	0.15894475299619992	0.12240572931891261	0.1534638994446068	0.028712791664538394
	0.22	0.5	5	0.5	0.2	0.01	3	2	10	100	0.062116340251388485	0.07490499853843906	0.07125109617071032	0.06942414498684595	0.006587166171192615
	0.22	0.5	5	0.5	0.2	1.0E-7	3	2	10	100	0.10961707103186204	0.014615609470914939	0.018269511838643674	0.047500730780473545	0.053825342964981863

Note	Effector	PFK-1 [µg]	FBPA [U]	TPI [U]	GPDH [U]	NADH [mM]	F6P [mM]	ADP [mM]	3-PG [mM]	MgCl2 [mM]	Buffer [mM]	spez. A1 [U/mg]	spez. A2 [U/mg]	spez. A3 [U/mg]	Mean [U/mg]	SD	relative activity in % for A1	relative activity in % for A2	relative activity in % for A3	Mean relative activity in %	Mean stabw
Buffer: HEPES/NaOH, pH7.7 at 30°C	3-PG	0.219	0.5	5	0.5	0.2	0.08	0.4	1.0E-8	10	100	2.308799130805033	2.529034342010601	2.4170814429811043	2.418304971932246	0.1101227035220757	100	100	100	100	0
30°C	3-PG	0.219	0.5	5	0.5	0.2	0.08	0.4	0.025	10	100	2.406069682420825	2.413410856127678	2.273928555697485	2.364469698081996	0.07849679647009657	104.21303656597773	95.42815674891146	94.07744874715262	97.9062140206806	5.503463544887433
PFK: phosphofructokinase	3-PG	0.219	0.5	5	0.5	0.2	0.08	0.4	0.1	10	100	1.462728861090311	1.8040934384589409	1.894022816367881	1.720281705305711	0.2275344455799024	63.35453100158982	71.33526850507982	78.35990888382686	71.01656946349884	7.5077638586006845
FBPA: fructose 1,6-bisphosphate aldolase	3-PG	0.219	0.5	5	0.5	0.2	0.08	0.4	0.15	10	100	1.0864937086141333	1.6370817366280521	1.5159523704649902	1.4131759385690585	0.289325146440831	47.05882352941177	64.73149492017416	62.71829916476841	58.169539204784776	9.674670092650754
TPI: triose phosphate isomerase	3-PG	0.219	0.5	5	0.5	0.2	0.08	0.4	0.25	10	100	0.46799982381183103	0.5120468660529446	0.5505880280139189	0.5102115726262315	0.0413246790042348	20.27027027027027	20.246734397677795	22.779043280182233	21.098682649376766	1.4552825744491624
GPDH: glycerol 3-phosphate dehydrogenase	3-PG	0.219	0.5	5	0.5	0.2	0.08	0.4	0.5	10	100	0.16701170183088873	0.1982116900850108	0.20371757036514998	0.1896469874270165	0.019795095224032354	7.233704292527822	7.837445573294629	8.428246013667424	7.8331319598299585	0.5972825431331749
	3-PG	0.219	0.5	5	0.5	0.2	0.1	0.4	1	10	100	0.038541161960974325	0.040376455387687395	0.053223509374678825	0.04404704224111352	0.007999858578786713	1.6693163751987286	1.5965166908563138	2.2019741837509486	1.822602416601997	0.33055582250563637

Note	PFK-1 [µg]	FBPA [U]	TPI [U]	GPDH [U]	NADH [mM]	F6P [mM]	ADP [mM]	3-PG [mM]	MgCl2 [mM]	Buffer [mM]	spez. A1 [U/mg]	spez. A2 [U/mg]	spez. A3 [U/mg]	Mean [U/mg]	SD
Buffer: HEPES/NaOH, pH7.7 at 30°C	2.49	0.5	5	0.5	0.2	3	1	2	10	100	7.057167577060655	7.2314983406294	6.966773847802786	7.085146588497612	0.13456181882807258
30°C	2.49	0.5	5	0.5	0.2	3	0.75	2	10	100	6.256537403633828	6.774687173129819	6.789214736760547	6.606813104508066	0.3034346099136755
PFK: phosphofructokinase	2.49	0.5	5	0.5	0.2	3	0.5	2	10	100	5.628623820038999	5.065277185914075	6.380828781363396	5.691576595772157	0.6600312788434314
FBPA: fructose 1,6-bisphosphate aldolase	2.49	0.5	5	0.5	0.2	3	0.25	2	10	100	2.485827554591356	2.288898358708145	2.2872841849713965	2.3540033660902995	0.11416594892823655
TPI: triose phosphate isomerase	2.49	0.5	5	0.5	0.2	3	0.15	2	10	100	0.9491341572076085	0.8877955552111986	0.9749609369955708	0.937296883138126	0.04477210893459093
GPDH: glycerol 3-phosphate dehydrogenase	2.49	0.5	5	0.5	0.2	3	0.1	2	10	100	0.6069293250171103	0.5972442825966245	0.5778741977556529	0.5940159351231292	0.014794146666911522
3-PG: 3-phosphoglycerate	2.49	0.5	5	0.5	0.2	3	0.05	2	10	100	0.37771665439894636	0.23889771303864982	0.21307093325068766	0.27656176689609463	0.08854935961281672
	2.49	0.5	5	0.5	0.2	3	0.025	2	10	100	0.07748033936388642	0.06133860199641008	0.07909451310063403	0.07263781815364351	0.009818635523279938
	2.49	0.5	5	0.5	0.2	3	0.01	2	10	100	0.033897648471700306	0.032283474734952675	0.033897648471700306	0.0333595905594511	9.319436414300692E-4
	2.49	0.5	5	0.5	0.2	3	1.0E-6	2	10	100	0.02259843231446687	0.014527563630728704	0.0016141737367476338	0.012913389893981069	0.010584845046265449

Note	Effector	PFK-1 [µg]	FBPA [U]	TPI [U]	GPDH [U]	NADH [mM]	F6P [mM]	ADP [mM]	3-PG [mM]	MgCl2 [mM]	Buffer [mM]	spez. A1 [U/mg]	spez. A2 [U/mg]	spez. A3 [U/mg]	Mean [U/mg]	SD	relative activity in % for A1	relative activity in % for A2	relative activity in % for A3	Mean relative activity in %	Mean stabw
Buffer: HEPES/NaOH, pH7.7 at 30°C	3-PG	2.2	0.5	5	0.5	0.2	0.4	0.4	1.0E-7	10	100	5.774992692195265	5.75672318035662	6.257307804735458	5.929674559095781	0.2838857190877998	100	100	100	100	0
30°C	3-PG	2.2	0.5	5	0.5	0.2	0.4	0.4	0.025	10	100	5.290850628471208	6.200672318035663	5.824320374159602	5.771947773555492	0.45716631948450437	91.61657703258463	107.71183751190101	93.0802919708029	97.46956883842951	8.900205954907587
PFK: phosphofructokinase	3-PG	2.2	0.5	5	0.5	0.2	0.4	0.4	0.1	10	100	5.616047939199066	5.04969307220111	5.000365390236773	5.222035467212316	0.34211500422054797	97.24770642201837	87.7181847032688	79.91240875912409	88.29276662813709	8.681920556914578
FBPA: fructose 1,6-bisphosphate aldolase	3-PG	2.2	0.5	5	0.5	0.2	0.4	0.4	0.15	10	100	4.174583455130079	4.349970768781058	5.21229172756504	4.578948650492059	0.5554572597460858	72.28725086997785	75.56331323389401	83.2992700729927	77.04994472562153	5.654528859775931
TPI: triose phosphate isomerase	3-PG	2.2	0.5	5	0.5	0.2	0.4	0.4	0.25	10	100	3.0016807950891558	3.209953230049693	3.8219818766442564	3.344538633927702	0.42638994981456735	51.97722239797533	55.76007616629642	61.080291970802925	56.27253017835823	4.573119913996742
GPDH: glycerol 3-phosphate dehydrogenase	3-PG	2.2	0.5	5	0.5	0.2	0.4	0.4	0.5	10	100	1.6643525285004384	1.856182402806197	2.0461853259280915	1.8555734190782422	0.19091712716187223	28.819993672888323	32.24373214852429	32.7007299270073	31.25481858280664	2.1209646387560657
	3-PG	2.2	0.5	5	0.5	0.2	0.4	0.4	1	10	100	0.1954837766734873	0.22106109324758844	0.19913767904121604	0.20522751632076394	0.013833451100556058	3.385004745333755	3.840050777530943	3.182481751824818	3.469179091563172	0.33676883215996684

F6P mM	ADP mM	ATP mM	Mg2+ mM	Specific activity 1 U/mg	Specific activity 2 U/mg	Specific activity 3 U/mg
0	3	0	5	0	1.0E-4	1.0E-4
0.005	3	0	5	0.566677567037279	1.1110856769130146	1.0018312622629166
0.01	3	0	5	1.3252943100065397	1.821451929365598	1.6877697841726615
0.025	3	0	5	3.5920045781556564	4.12558862001308	4.061478090255067
0.05	3	0	5	5.767315238718115	6.156507521255722	6.390058862001307
0.1	3	0	5	9.377599738391103	9.034401569653365	9.305297580117722
0.2	3	0	5	13.061003924133416	13.169097449313275	13.123086984957483
0.3	3	0	5	13.609401569653365	14.353041203400911	14.494964028776973
0.4	3	0	5	15.016955526487894	14.735546108567686	14.901111837802482
0.6	3	0	5	15.345994113799863	14.671795291039892	14.684499672988876
0.8	3	0	5	14.203499018966644	14.006965336821448	14.467887508175275
1	3	0	5	12.110448005232175	11.356752779594505	11.227730542838453



F6P mM	ADP mM	ATP mM	Mg2+ mM	Specific activity 1 U/mg	Specific activity 2 U/mg	Specific activity 3 U/mg
0	3	1	5	0	1.0E-4	1.0E-4
0.005	3	1	5	0.8122956180510136	0.165892740353172	0.2346631785480706
0.01	3	1	5	2.3285807717462386	0.4976782210595159	0.505428384565075
0.025	3	1	5	3.7726618705035966	2.2351863963374745	2.1300196206671025
0.05	3	1	5	6.985742315238716	4.426716808371484	4.4586003924133415
0.1	3	1	5	9.684368868541528	6.8889143230869845	7.61752779594506
0.2	3	1	5	13.926357096141269	10.643328973185085	10.668149117069976
0.3	3	1	5	15.027468933943751	12.965827338129493	12.56350555918901
0.4	3	1	5	15.578024852844992	14.441399607586655	13.511183780248528
0.6	3	1	5	15.496795291039895	15.340712884238062	15.198953564421188
0.8	3	1	5	15.523871811641593	15.384368868541527	15.388489208633091
1	3	1	5	13.72779594506213	14.677141922825372	13.484107259646825



F6P mM	ADP mM	ATP mM	Mg2+ mM	Specific activity 1 U/mg	Specific activity 2 U/mg	Specific activity 3 U/mg
0	3	2	5	0	1.0E-4	1.0E-4
0.005	3	2	5	0.026193590582079818	0.21661216481360357	0.017462393721386534
0.01	3	2	5	0.4714846304774362	0.297841726618705	0.2968606932635709
0.025	3	2	5	0.8381948986265533	0.44224983649444066	0.4540222367560495
0.05	3	2	5	1.1612491824722038	1.3899280575539568	1.4057226945716152
0.1	3	2	5	4.033812949640287	3.4477436232831913	4.007619359058207
0.2	3	2	5	7.098463047743623	6.3449313276651385	7.089731850882928
0.3	3	2	5	9.455886200130802	8.222236756049703	9.490810987573576
0.4	3	2	5	10.870340091563111	9.467756703727924	10.486167429692607
0.6	3	2	5	12.180019620667101	11.047220405493784	12.267331589274033
0.8	3	2	5	12.782472204054931	11.669980379332895	12.354643557880967
1	3	2	5	12.703891432308698	11.787311968606932	12.668966644865925

ADP mM	F6P mM	ATP mM	Mg2+ mM	Specific activity 1 U/mg	Specific activity 2 U/mg	Specific activity 3 U/mg
0	0.8	0	5	0	1.0E-4	1.0E-4
0.05	0.8	0	5	2.8003597122302155	4.310856769130149	3.0379659908436882
0.1	0.8	0	5	5.634663178548069	5.846811641595814	6.432341399607586
0.2	0.8	0	5	7.94283845650752	8.621713538260298	8.333191628515367
0.35	0.8	0	5	9.801258992805753	10.92140287769784	10.25949967298888
0.5	0.8	0	5	12.796795291039896	12.067004578155654	12.134892086330932
1	0.8	0	5	14.774018966644864	14.341236102027462	14.086657946370174
2	0.8	0	5	15.469865925441463	15.715958142576845	15.104970568999343
3	0.8	0	5	15.588669064748196	15.987508175277952	14.918279921517328
4	0.8	0	5	15.334090909090907	15.60564094179202	14.87585022890778
5	0.8	0	5	15.045568999345972	15.478351863963372	14.84190647482014
6	0.8	0	5	15.682014388489204	15.045568999345972	14.392151733158926



ADP mM	F6P mM	ATP mM	Mg2+ mM	Specific activity 1 U/mg	Specific activity 2 U/mg	Specific activity 3 U/mg
0	0.8	1	5	0	1.0E-4	1.0E-4
0.05	0.8	1	5	0.025261608894702414	0.39576520601700443	0.1272890778286461
0.1	0.8	1	5	0.08420536298234138	0.7746893394375406	0.41581098757357754
0.2	0.8	1	5	1.2967625899280573	1.2041366906474817	1.0777141922825373
0.35	0.8	1	5	3.418737737083059	3.065075212557226	2.9615925441465003
0.5	0.8	1	5	5.65860039241334	4.858649444081097	5.337655330281228
1	0.8	1	5	10.03727926749509	9.422580117724001	10.098266841072595
2	0.8	1	5	15.392740353172002	14.00335186396337	15.622612818835837
3	0.8	1	5	16.942119032047085	15.52746893394375	17.048250490516672
4	0.8	1	5	17.093688685415298	15.982177894048391	17.16705362982341
5	0.8	1	5	16.487410071942442	15.148544800523213	17.28585676913015
6	0.8	1	5	16.54635382603008	15.359058207979068	16.742756703727924



ADP mM	F6P mM	ATP mM	Mg2+ mM	Specific activity 1 U/mg	Specific activity 2 U/mg	Specific activity 3 U/mg
0	0.8	2	5	0	1.0E-4	1.0E-4
0.05	0.8	2	5	0.12728907782864615	0.07578482668410723	0.44126880313930666
0.1	0.8	2	5	0.18669064748201433	0.15156965336821446	0.526128188358404
0.2	0.8	2	5	0.2545781556572923	0.2189339437540876	0.4242969260954871
0.35	0.8	2	5	0.6873610202746891	0.6483812949640286	0.7128188358404184
0.5	0.8	2	5	1.5104970568999345	1.524117069980379	1.5189829954218441
1	0.8	2	5	5.64314911706998	5.751226291693915	5.846811641595814
2	0.8	2	5	11.22689666448659	11.300359712230211	11.88031393067364
3	0.8	2	5	13.721762589928055	14.399117069980374	13.747220405493783
4	0.8	2	5	14.757047089601045	15.418001962066706	14.485497056899934
5	0.8	2	5	15.13042838456507	15.67061805101373	14.95222367560497
6	0.8	2	5	14.723103335513404	15.232750163505555	15.071026814911704




























µg	F6P mM	ADP mM		NADH mM	1 MHEPES µL	H2O µL	Specific activity 1 U/mg
	0.8	0.5		0.7			16.287410071942443
	0.8	0.5		0.7			6.489372138652711
	0.8	0.5		0.7			1.4075539568345319
	0.8	1		0.7			18.39874100719424
	0.8	1		0.7			12.256687377370827
	0.8	1		0.7			7.065189666448657

F6P mM	ADP mM	ATP mM	Specific activity 1 U/mg	Specific activity 2 U/mg	Specific activity 3 U/mg
0.25	0.5	0	9.296272073250488	8.637410071942444	9.052076520601698
0.25	0.5	0.05	8.42079790712884	8.303466317854804	10.003597122302153
0.25	0.5	0.1	8.168083714846302	7.617527795945059	8.487900588620011
0.25	0.5	0.25	6.083191628515368	5.830477436232829	6.450130804447349
0.25	0.5	0.5	3.348463047743622	3.8566056245912352	3.578727926749509
0.25	0.5	1	1.1281883584041856	1.2293982995421842	1.591481360366252
0.25	0.5	2	0.31589274035317194	0.4126062786134727	0.2189339437540876
0.25	0.5	3	0.17148463047743623	0.27787769784172656	0.12630804447351204



F6P mM	ADP mM	ATP mM	Specific activity 1 U/mg	Specific activity 2 U/mg	Specific activity 3 U/mg
0.07	0.2	0	2.7703564421190316	2.8124591236102017	2.19775997383911
0.07	0.2	0.05	1.9788260300850224	2.0377697841726614	1.6925277959450615
0.07	0.2	0.1	1.5241170699803788	1.4651733158927398	1.1620340091563113
0.07	0.2	0.25	0.5810170045781555	0.6399607586657945	0.49681164159581426
0.07	0.2	0.5	1.1536134728580771	0.2189339437540876	0.34524198822759955
0.07	0.2	1	0.5473348593852191	0.15156965336821446	0.1684107259646828
0.07	0.2	2	0.23577501635055584	0.12630804447351204	0.12630804447351204
0.07	0.2	3	0.4041857423152386	0.22735448005232164	0.10946697187704378



F6P mM	ADP mM	ATP mM	Specific activity 1 U/mg	Specific activity 2 U/mg	Specific activity 3 U/mg
0.05	0.5	0	3.7724002616088934	3.839764551994767	4.058698495748854
0.05	0.5	0.05	3.258747547416611	2.997710922171353	3.250327011118377
0.05	0.5	0.1	2.719833224329627	2.593525179856114	2.728253760627861
0.05	0.5	0.25	2.113554610856768	1.3893884892086326	1.4988554610856766
0.05	0.5	0.5	0.5810170045781555	0.5810170045781555	0.8083714846304773
0.05	0.5	1	0.1936723348593852	0.4294473512099411	0.3284009156311314
0.05	0.5	2	0.3873446697187703	0.49681164159581426	-0.04210268149117067
0.05	0.5	3	0.3368214519293655	0.4041857423152386	0.3199803793328972




F6P mM	ADP mM	ATP mM	Specific activity 1 U/mg	Specific activity 2 U/mg	Specific activity 3 U/mg
0.8	5	0	13.329708960104638	14.59581425768476	14.660153695225633
0.8	5	0.05	13.801258992805751	13.95088293001962	14.239126880313927
0.8	5	0.1	12.647645519947675	13.891481360366251	14.988554610856765
0.8	5	0.25	12.35292674950948	13.781164159581424	14.197024198822758
0.8	5	0.5	12.521337475474162	12.907112491824721	14.3064911706998
0.8	5	1	9.616252452583387	11.328727926749508	11.889797253106602
0.8	5	2	9.372056899934595	11.685137344669718	12.16767495094833
0.8	5	3	7.696370176586003	9.012066710268147	8.302648790058859