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Organism_Sample
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Data_flux
Results_MDA-mb-231_IAA
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| Metadata | Values (examples) | ||||||||
| Asset Title | MDA-mb-231_Iodoacetic titration in intact cells: GAPDH and flux inhibition data | ||||||||
| Uploader | Theresa Kouril | ||||||||
| Uploader SEEK ID | |||||||||
| Project | Project | Glucose metabolism in cancer cells | |||||||
| ASSAY | |||||||||
| Assay SEEK ID | |||||||||
| Assay Title | GAPDH and flux inhibition in intact cells | ||||||||
| Assay_type | enzymaticAssay | extracellular metabolite concentration | protein concentration | ||||||
| Technology_type | plate reader based assays | plate reader based assays | Bradford | ||||||
| Description | |||||||||
| Experimentalist | Craig October | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | gas_flow | growth_medium | ||||||
| Compound (if concentration) | CO2 | DMEM | |||||||
| Unit | °C | % | |||||||
| Start_value (optional) | 37 | 5 | low glucose | ||||||
| End_value (optional) | |||||||||
| Comments | |||||||||
| Culture growth | Batch | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | FactorsStudied | FactorsStudied | |||||||
| Compound (if concentration) | Iodoacetic acid | ||||||||
| Unit | mM | ||||||||
| Start_value (optional) | 0 | ||||||||
| End_value (optional) | 1.2 | ||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
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| Sample Name | chatacteristics[Treatments (Factors Studied)] | experimental details | comment [replica type] | comment[date_experiment] | |||||
| organism | chatacteristics [cell line] | specific_concentration | volume | date | |||||
| 1 | Homo sapiens | MDA-mb-231 | 0 -0.4 mM IAA | 10 ml LG DMEM, 100 mm dish | biological | 2021-10-11T0:0:0+0000 | |||
| 2 | Homo sapiens | MDA-mb-231 | 0 -0.4 mM IAA | 10 ml LG DMEM, 100 mm dish | biological | 2021-10-13T0:0:0+0000 | |||
| 3 | Homo sapiens | MDA-mb-231 | 0 -0.4 mM IAA | 10 ml LG DMEM, 100 mm dish | biological | 2021-12-10T0:0:0+0000 | |||
| 4 | Homo sapiens | MDA-mb-231 | 0 -0.4 mM IAA | 10 ml LG DMEM, 100 mm dish | biological | 2022-03-13T0:0:0+0000 | |||
| 5 | Homo sapiens | MDA-mb-231 | 0 -0.4 mM IAA | 10 ml LG DMEM, 100 mm dish | biological | 2022-03-14T0:0:0+0000 | |||
| 6 | Homo sapiens | MDA-mb-231 | 0, 0.1 - 1.2 mM IAA | 10 ml LG DMEM, 100 mm dish | biological | 2022-11-16T0:0:0+0000 | |||
| 7 | Homo sapiens | MDA-mb-231 | 0, 0.01, 0.025, 0.05, 0.6 - 1.2 mM IAA | 10 ml LG DMEM, 100 mm dish | biological | 2022-11-16T0:0:0+0000 | |||
| 8 | Homo sapiens | MDA-mb-231 | 0, 0.2 - 0.8 mM IAA | 10 ml LG DMEM, 100 mm dish | biological | 2022-10-19T0:0:0+0000 | |||
| Note: this sheet allows you to describe your samples in as much detail as required. Sample names can be used in the data matrix file to relate different conditions to different experimental measurements | |||||||||
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| [IAA] | sample | sample | sample | sample | sample | sample | sample | sample | |
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | ||
| uM | mg/ml | mg/ml | mg/ml | mg/ml | mg/ml | mg/ml | mg/ml | mg/ml | |
| 0 | 3.8625837603128144 | 4.636339768407569 | 7.690300400816865 | 6.167144798641388 | 4.428908541047367 | 8.72832436006808 | 10.570428728731335 | 5.484970513311271 | |
| 5 | 4.018079241518391 | 4.334736105583975 | 7.661921094119668 | 0 | 4.2999526845765645 | ||||
| 10 | 3.447839700473546 | 3.536716436946988 | 7.954125498643775 | 5.324378536441103 | 4.164811444027832 | 11.223211868216469 | |||
| 25 | 3.9580987912021293 | 4.42844347005594 | 7.53785436701369 | 5.484545150587725 | 3.845706338968304 | 10.768061923666004 | |||
| 50 | 4.102207517079407 | 4.839157294681257 | 8.943939207428558 | 5.525341520787339 | 3.894042468413163 | 9.855528658745186 | |||
| 100 | 4.22759945654218 | 5.3214290466127245 | 8.340506056371721 | 5.089644897170394 | 4.248072714824942 | 9.155175063873044 | |||
| 200 | 3.1966270417364075 | 4.020926396940334 | 6.882353291927547 | 4.769833027165231 | 3.5254790242710143 | 10.58385934155524 | 5.1679933711815655 | ||
| 400 | 1.900970375849849 | 2.497376268258997 | 8.144588717974651 | 5.341004045781581 | 3.458364714371062 | 10.729425614401563 | 5.546693331413742 | ||
| 600 | 10.637289036275247 | 9.908911769853916 | 5.0357285171262856 | ||||||
| 800 | 9.512645528237405 | 9.684942769043424 | 4.582287543778783 | ||||||
| 1000 | 10.31569334945769 | 9.424754233128011 | |||||||
| 1200 | 7.449566526670317 | 8.752739248830201 | |||||||
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| Enzymatic Assay | |||||||||||||||||||||||||||||
| Enzyme | [IAA] | sample | sample | sample | sample | sample | sample | sample | sample | ||||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | ||||||||||||||||||||||
| (μM) | activity (nmol/min/mg) | activity (nmol/min/mg) | activity (nmol/min/mg) | activity (nmol/min/mg) | activity (nmol/min/mg) | activity (nmol/min/mg) | activity (nmol/min/mg) | activity (nmol/min/mg) | |||||||||||||||||||||
| GAPDH | 0 | 346.3584595886388 | 432.6973666750887 | 335.324317136722 | 305.30143077479346 | 329.08992220879895 | 468.9876171591804 | 400.7091878796967 | 619.5518467425306 | ||||||||||||||||||||
| 5 | 254.92092698916423 | 317.0883913862301 | 247.08266384708074 | 0 | 278.57275676431874 | ||||||||||||||||||||||||
| 10 | 225.671457983813 | 192.6261328014401 | 189.23747148285884 | 139.18719131305212 | 170.11089775372366 | 189.4007408782592 | |||||||||||||||||||||||
| 25 | 132.6548479135049 | 130.35718772452495 | 89.24113923844165 | 70.63999411379379 | 126.5639309366787 | 122.97923194409995 | |||||||||||||||||||||||
| 50 | 72.3419105685041 | 127.30880515588613 | 80.28730085560194 | 46.793871034861695 | 100.19101612571946 | 89.0062041053042 | |||||||||||||||||||||||
| 100 | 57.709285009741386 | 81.57476611091477 | 64.81368461854957 | 55.74732671514093 | 62.46057304519668 | 126.26021051059774 | |||||||||||||||||||||||
| 200 | 90.38445414780713 | 91.10456210686992 | 47.26294923981339 | 49.12291371639922 | 43.46837622871367 | 62.22389612798108 | 51.46210489362864 | ||||||||||||||||||||||
| 400 | 22.040609656506746 | 48.20859167064742 | 40.66007655626939 | 17.683546404889956 | 37.00259533161603 | 27.587535966111574 | 31.234557069546234 | ||||||||||||||||||||||
| 600 | 37.71268434139314 | 68.35704792186591 | 33.74619841624764 | ||||||||||||||||||||||||||
| 800 | 37.957620040938586 | 31.315449771448574 | 24.272068008389486 | ||||||||||||||||||||||||||
| 1000 | 37.92202339160686 | 50.76919811232191 | |||||||||||||||||||||||||||
| 1200 | 21.41865049460599 | 41.82831699393319 | |||||||||||||||||||||||||||
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| Flux | |||||||||
| Metabolite | [IAA] | sample | sample | sample | sample | sample | sample | sample | sample |
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | ||
| (μM) | flux (nmol/min/mg) | flux (nmol/min/mg) | flux (nmol/min/mg) | flux (nmol/min/mg) | flux (nmol/min/mg) | flux (nmol/min/mg) | flux (nmol/min/mg) | flux (nmol/min/mg) | |
| Lactate | 0 | 54.08768994756163 | 55.33202604929837 | 26.211990540415158 | 38.06172656994264 | 57.24188811831377 | 56.67738324925587 | 61.85580637905123 | 96.84403012155806 |
| 5 | 50.01015592935731 | 51.896794768371194 | 23.510503351052545 | - | 53.105383980829515 | ||||
| 10 | 55.88548454424428 | 55.39994471545555 | 24.769377767055296 | 40.55290700343153 | 60.469320421021806 | 51.2673428785143 | |||
| 25 | 42.81976950845919 | 42.017425699158636 | 22.566883243926597 | 37.06523817734018 | 57.472367075475894 | 43.659260904755186 | |||
| 50 | 43.50787279862477 | 47.590681412562084 | 21.82087305312292 | 36.82659471720714 | 55.53611993585343 | 42.38674304934079 | |||
| 100 | 35.04396784747123 | 35.32783866858946 | 19.750934115850995 | 34.17840600624648 | 47.235845162520064 | 32.6580861163067 | |||
| 200 | 40.95519398539867 | 44.243323396557976 | 17.154119061879275 | 32.79461910833437 | 34.91764829121013 | 23.647427521022323 | 54.25603785769206 | ||
| 400 | 38.71267323454869 | 13.24271336837289 | 9.859101874389328 | 19.9622213073745 | 23.606644380862605 | 6.554964237417245 | 56.72494275508909 | ||
| 600 | 5.138784618408757 | 6.224531327463668 | 33.78030452114699 | ||||||
| 800 | 1.0147833895987732 | 3.825441012257311 | 22.254195033088134 | ||||||
| 1000 | 2.547199946735394 | 3.3915645489863633 | |||||||
| 1200 | 3.3480640733358893 | 2.3530217991215094 | |||||||
| Glucose | 0 | 31.04509891614327 | 28.670703864611614 | 27.245120798201814 | 22.281203762093973 | 29.3294389415643 | 24.98605112289166 | 29.234988066255426 | 38.6884585895004 |
| 5 | 28.771699116863356 | 32.218585249646395 | 26.43059771631066 | - | 28.174565046370773 | ||||
| 10 | 24.285855684530425 | 32.1050600103864 | 24.266592917374055 | 29.647689232907656 | 28.42849802117598 | 23.142914695116453 | |||
| 25 | 29.842521262961025 | 26.769704515054244 | 23.373951970669502 | 23.215302240326288 | 27.784007532664212 | 16.628641575366224 | |||
| 50 | 23.307788999263252 | 25.74479007157602 | 20.811152814296573 | 28.83111621687926 | 25.266320813487788 | 17.132396260942702 | |||
| 100 | 20.804339038925754 | 19.606950461067154 | 20.03765075895749 | 22.440465325165796 | 16.883590763347655 | 12.899375419949875 | |||
| 200 | 18.163565134391224 | 21.667443339324322 | 15.569378727151362 | 12.390448196945893 | 24.03309458447375 | 9.346880755633421 | 7.33845693872681 | ||
| 400 | 10.39557099445224 | 8.894955063696234 | 10.34167253614692 | 9.990580398140446 | 19.881586016308166 | 4.292858931955612 | 4.54241555149335 | ||
| 600 | 0.008724591881847899 | 3.1381580569409313 | 0.0949268858500103 | ||||||
| 800 | 1.5946596122266532 | 1.0815382966591092 | -6.01928653394853 | ||||||
| 1000 | 2.067935845259059 | 4.503635417422539 | |||||||
| 1200 | 2.769538295958014 | 5.228937331381129 | |||||||
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| Sample | [IAA] (μM) | Relative Flux | Relative Activity | ||||||
| Lactate | Glucose | GAPDH | |||||||
| 1 | 0 | 1 | 1 | 1 | |||||
| 5 | 0.9246125315731266 | 0.9267710563454588 | 0.7360031780136896 | ||||||
| 10 | 1.0332385169051521 | 0.7822766405135183 | 0.6515546299975965 | ||||||
| 25 | 0.7916731062090697 | 0.9612635264448485 | 0.38299872355090075 | ||||||
| 50 | 0.8043951006376115 | 0.7507719354420654 | 0.20886428082173236 | ||||||
| 100 | 0.6479102339450361 | 0.6701327992260839 | 0.1666172238965413 | ||||||
| 200 | 0.7571999104621588 | 0.5850702934931309 | 0.2609563925626487 | ||||||
| 400 | 0.7157390761572713 | 0.3348538531808834 | 0.06363525719188098 | ||||||
| 2 | 0 | 1 | 1 | 1 | |||||
| 5 | 0.9379160401994581 | 1.123745876689618 | 0.732817936524053 | ||||||
| 10 | 1.0012274747737715 | 1.1197862515685857 | 0.4451751908767278 | ||||||
| 25 | 0.7593690074121446 | 0.9336954070421765 | 0.3012664225951017 | ||||||
| 50 | 0.860092875871939 | 0.8979476120693687 | 0.2942213541398369 | ||||||
| 100 | 0.6384699999438648 | 0.6838670774758379 | 0.18852614412180846 | ||||||
| 200 | 0.7995970246442656 | 0.7557346147357251 | 0.21055030403103908 | ||||||
| 400 | 0.23933179957253367 | 0.310245437492566 | 0.11141410922162401 | ||||||
| 3 | 0 | 1 | 1 | 1 | |||||
| 5 | 0.8969369691631277 | 0.9701038917050824 | 0.7368468411622459 | ||||||
| 10 | 0.9449636313909255 | 0.8906766498526832 | 0.5643416293179265 | ||||||
| 25 | 0.8609374098900149 | 0.8579133175365546 | 0.26613381337940756 | ||||||
| 50 | 0.8324767636200021 | 0.7638487995131266 | 0.23943178813025465 | ||||||
| 100 | 0.7535076012406561 | 0.7354583195784539 | 0.19328656260894744 | ||||||
| 200 | 0.6544378625282221 | 0.5714556687955278 | 0.1409469782668426 | ||||||
| 400 | 0.3761294610261665 | 0.37957888360067293 | 0.12125597363012307 | ||||||
| 4 | 0 | 1 | 1 | 1 | |||||
| 10 | 1.0654510622084121 | 1.3306143397577987 | 0.4559008811711859 | ||||||
| 25 | 0.9738191489875976 | 1.0419231603555215 | 0.23137786788133857 | ||||||
| 50 | 0.9675492426633404 | 1.2939658253980137 | 0.15327105056831308 | ||||||
| 100 | 0.8979730844169634 | 1.00714779886995 | 0.18259765954475043 | ||||||
| 200 | 0.8616166964488756 | 0.5560942007103411 | 0.1608997166889627 | ||||||
| 400 | 0.5244696735102572 | 0.44838602549548867 | 0.05792159689528045 | ||||||
| 5 | 0 | 1 | 1 | 1 | |||||
| 5 | 0.9277364134297165 | 0.9606240713470692 | 0.8464943408007846 | ||||||
| 10 | 1.0563823523088063 | 0.9692820267655529 | 0.5169131178857331 | ||||||
| 25 | 1.004026403823119 | 0.947307842745332 | 0.3845876837771325 | ||||||
| 50 | 0.970200700247087 | 0.8614662170602093 | 0.3044487520409418 | ||||||
| 100 | 0.8251971888992878 | 0.575653383516349 | 0.1897978905764458 | ||||||
| 200 | 0.6100016865103843 | 0.819418831446352 | 0.1320866222124363 | ||||||
| 400 | 0.41240156739885697 | 0.6778713379386511 | 0.11243916277733612 | ||||||
| 6 | 0 | 1 | 1 | 1 | |||||
| 100 | 0.5762101960967909 | 0.5162630684018636 | 0.26921864435440607 | ||||||
| 200 | 0.41722863980903346 | 0.374083952268472 | 0.1326770555369727 | ||||||
| 400 | 0.11565396744923483 | 0.17181021966382606 | 0.05882359140571511 | ||||||
| 600 | 0.09066728779289975 | 3.491785011939967E-4 | 0.08041296392819892 | ||||||
| 800 | 0.01790455612842882 | 0.06382199429527527 | 0.08093522867588906 | ||||||
| 1000 | 0.04494208802007169 | 0.0827636121885807 | 0.0808593276328139 | ||||||
| 1200 | 0.059072312118076606 | 0.11084337746434117 | 0.0456699701888637 | ||||||
| 7 | 0 | 1 | 1 | 1 | |||||
| 10 | 0.8288202172056246 | 0.7916170392362601 | 0.42656836404219933 | ||||||
| 25 | 0.7058231629414391 | 0.5687924871965272 | 0.30690394845905433 | ||||||
| 50 | 0.6852508362690418 | 0.5860237131657268 | 0.22212169522807693 | ||||||
| 600 | 0.10062970142721697 | 0.10734254619255895 | 0.17059016860474002 | ||||||
| 800 | 0.061844493446825015 | 0.03699465497328114 | 0.07815006672831841 | ||||||
| 1000 | 0.054830172744057666 | 0.1540495042178888 | 0.12669836292239983 | ||||||
| 1200 | 0.03804043527784984 | 0.17885888372968592 | 0.10438571976665316 | ||||||
| 8 | 0 | 1 | 1 | 1 | |||||
| 200 | 0.5602414293332298 | 0.18968077835797734 | 0.08306343555298115 | ||||||
| 400 | 0.5857350492734376 | 0.11741009378766279 | 0.05041475904522078 | ||||||
| 600 | 0.348811428838165 | 0.002453622845438778 | 0.054468723793945316 | ||||||
| 800 | 0.22979418561118117 | -0.1555835190493243 | 0.039176814879992305 |
