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Data_enzyme activity
Data_flux
Result_MCF7_IAA
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| Metadata | Values (examples) | ||||||||
| Asset Title | MCF7_Iodoacetic titration in intact cells: GAPDH and flux inhibition data | ||||||||
| Uploader | Theresa Kouril | ||||||||
| Uploader SEEK ID | 261 | ||||||||
| Project | Project | Glucose metabolism in cancer cells | |||||||
| ASSAY | |||||||||
| Assay SEEK ID | |||||||||
| Assay Title | GAPDH and flux inhibitiom in intact cells | ||||||||
| Assay_type | enzymaticAssay | extracellular metabolite concentration | protein concentration | ||||||
| Technology_type | plate reader based assays | plate reader based assays | Bradford | ||||||
| Description | |||||||||
| Experimentalist | Stephanie Hollocks | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | gas_flow | growth_medium | ||||||
| Compound (if concentration) | CO2 | DMEM | |||||||
| Unit | °C | % | |||||||
| Start_value (optional) | 37 | 5 | low glucose | ||||||
| End_value (optional) | |||||||||
| Comments | |||||||||
| Culture growth | Batch | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | FactorsStudied | FactorsStudied | |||||||
| Compound (if concentration) | Iodoacetic acid | ||||||||
| Unit | mM | ||||||||
| Start_value (optional) | 0 | ||||||||
| End_value (optional) | 0.4 | ||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
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| Sample Name | chatacteristics[Treatments (Factors Studied)] | experimental details | comment [replica type] | comment[date_experiment] | |||||
| organism | chatacteristics [cell line] | specific_concentration | volume | date | |||||
| 1 | Homo sapiens | MCF7 | 0 -0.4 mM IAA | 7 ml LG DMEM, 100 mm dish | biological | 2021-11-04T0:0:0+0000 | |||
| 2 | Homo sapiens | MCF7 | 0 -0.4 mM IAA | 7 ml LG DMEM, 100 mm dish | biological | 2021-11-24T0:0:0+0000 | |||
| 3 | Homo sapiens | MCF7 | 0 -0.4 mM IAA | 7 ml LG DMEM, 100 mm dish | biological | 2021-12-09T0:0:0+0000 | |||
| Note: this sheet allows you to describe your samples in as much detail as required. Sample names can be used in the data matrix file to relate different conditions to different experimental measurements | |||||||||
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| [IAA] | sample | sample | sample | ||||||
| 1 | 2 | 3 | |||||||
| uM | mg/ml | mg/ml | mg/ml | ||||||
| 0 | 2.639 | 2.6568820891948137 | 2.168484296987319 | ||||||
| 5 | 2.522 | 2.1494876413686534 | 2.1141305576818077 | ||||||
| 10 | 2.661 | 2.6426385687674783 | 1.954505428917407 | ||||||
| 25 | 2.642 | 2.5038543980336607 | 1.901159218480338 | ||||||
| 50 | 2.958 | 2.530019381681866 | 1.857212163965615 | ||||||
| 100 | 2.262 | 2.1090654872505885 | 1.7838239648053782 | ||||||
| 200 | 1.91 | 1.966756556835668 | 1.6670658653808004 | ||||||
| 400 | 2.343 | 2.1525022028277325 | 1.7952014484815777 | ||||||
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| Enzymatic Assay | |||||||||||||||||||||||||||||
| Enzyme | [IAA] | sample | sample | sample | |||||||||||||||||||||||||
| 1 | 2 | 3 | |||||||||||||||||||||||||||
| (μM) | activity (nmol/min/mg) | activity (nmol/min/mg) | activity (nmol/min/mg) | ||||||||||||||||||||||||||
| GAPDH | 0 | 667.6827965254314 | 679.3958308880714 | 829.0862433851888 | |||||||||||||||||||||||||
| 5 | 513.7405021598445 | 512.5400060783202 | 509.6561281163754 | ||||||||||||||||||||||||||
| 10 | 342.5111532208985 | 296.97312943220516 | 322.4367961863241 | ||||||||||||||||||||||||||
| 25 | 115.07811737023641 | 131.09477516959777 | 157.2043376557634 | ||||||||||||||||||||||||||
| 50 | 73.68731874845048 | 89.00463224401346 | 109.87241938545837 | ||||||||||||||||||||||||||
| 100 | 57.16894259251295 | 61.080834944319065 | 68.46228233027168 | ||||||||||||||||||||||||||
| 200 | 40.46290726936949 | 28.558205377822333 | 40.49240368991739 | ||||||||||||||||||||||||||
| 400 | 39.36123144497177 | 24.896870251570363 | 31.287317490635417 | ||||||||||||||||||||||||||
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| Flux | |||||||||
| Metabolite | [IAA] | sample | sample | sample | |||||
| 1 | 2 | 3 | |||||||
| (μM) | flux (nmol/min/mg) | flux (nmol/min/mg) | flux (nmol/min/mg) | ||||||
| Lactate | 0 | 50.09639254637456 | 34.217814476008655 | 40.02988021968336 | |||||
| 5 | 52.03207414555557 | 40.133225870023864 | 41.216707115547415 | ||||||
| 10 | 50.030291650693265 | 36.99705378758661 | 40.90975248793345 | ||||||
| 25 | 51.66760929685723 | 39.33300876499409 | 49.13668763699751 | ||||||
| 50 | 39.799850015002754 | 39.94887521543014 | 47.33573347499766 | ||||||
| 100 | 37.13799054812232 | 34.72349598759788 | 41.222303760985795 | ||||||
| 200 | 14.4244108658343 | 16.37334363546836 | 20.007707769271445 | ||||||
| 400 | 4.967933627902417 | 5.986019720673814 | 5.90230881458765 | ||||||
| Glucose | 0 | 31.572175652394424 | 23.235553898003786 | 27.075363230238484 | |||||
| 5 | 34.83566283897878 | 26.929636403056175 | 28.678061743333362 | ||||||
| 10 | 34.21773858798198 | 25.577190403602675 | 26.95694737936 | ||||||
| 25 | 36.25407005210187 | 27.956897196167933 | 33.16397668702258 | ||||||
| 50 | 26.85908830600151 | 25.907951347015118 | 30.5004643154933 | ||||||
| 100 | 29.235232430493106 | 23.65261815551845 | 23.40944743271757 | ||||||
| 200 | 15.907640774917516 | 15.65303757534979 | 15.798715903755753 | ||||||
| 400 | 11.815289533790821 | 10.255690700988122 | 10.42594227099006 | ||||||
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| Sample | [IAA] (μM) | Relative Flux | Relative Activity | ||||||
| Lactate | Glucose | GAPDH | |||||||
| 1 | 0 | 1 | 1 | 1 | |||||
| 5 | 1.0386391414788827 | 1.1033659264573632 | 0.7694379798810296 | ||||||
| 10 | 0.9986805258358652 | 1.0837941282449097 | 0.5129848410102814 | ||||||
| 25 | 1.0313638701435075 | 1.1482917886703312 | 0.17235447426396763 | ||||||
| 50 | 0.7944653894621209 | 0.8507202228226716 | 0.1103627637733269 | ||||||
| 100 | 0.7413306360082402 | 0.9259809254949434 | 0.08562290789880407 | ||||||
| 200 | 0.28793312517426334 | 0.503850002295014 | 0.060601991664208314 | ||||||
| 400 | 0.09916749241581753 | 0.3742310844800698 | 0.058951992847209055 | ||||||
| 2 | 0 | 1 | 1 | 1 | |||||
| 5 | 1.1728751962859205 | 1.1589840518228298 | 0.754405580335037 | ||||||
| 10 | 1.0812219995384738 | 1.1007781659037645 | 0.4371135587394128 | ||||||
| 25 | 1.1494892168689466 | 1.203194781535626 | 0.19295787405441892 | ||||||
| 50 | 1.1674876326025947 | 1.1150132878580064 | 0.13100556140839306 | ||||||
| 100 | 1.0147783112198405 | 1.017949400274486 | 0.0899046361006916 | ||||||
| 200 | 0.4785034896646687 | 0.6736675030025677 | 0.04203470801475557 | ||||||
| 400 | 0.1749386923840746 | 0.4413792219461232 | 0.03664560351956598 | ||||||
| 3 | 0 | 1 | 1 | 1 | |||||
| 5 | 1.029648524785754 | 1.0591939801311674 | 0.6147202805288762 | ||||||
| 10 | 1.0219803872362687 | 0.9956264353733125 | 0.38890621905605754 | ||||||
| 25 | 1.2275002415030003 | 1.2248765198460634 | 0.18961156201783358 | ||||||
| 50 | 1.1825099954139229 | 1.1265024980876184 | 0.13252230423803119 | ||||||
| 100 | 1.029788336481609 | 0.8646032643644566 | 0.08257558592546142 | ||||||
| 200 | 0.4998193264498783 | 0.5835089180303712 | 0.048839796840176065 | ||||||
| 400 | 0.1474475762154638 | 0.3850711874973515 | 0.03773710846158559 | ||||||
