Oxygen concentration and consumption flux in permeabilised HepG2 cells

Selected Cell

Cell:

Value:

AY1_20210129_lWT_rKO_PCoAMalCar AY2_20210129_lWT_rKO_PCoAMalCar AZ1_20210219_lWT_rKO_PCoAMalCar AZ2_20210219_lWT_rKO_PCoAMalCar

100

Time [min]	Event Name	Chamber	Event Text	1A: O2 concentration [µM]	1A: O2 slope neg. [pmol/(s*mL)]	1B: O2 concentration [µM]	1B: O2 slope neg. [pmol/(s*mL)]
0				188.1336		188.1712
0.03				188.1054		188.1521
0.07				188.0941		188.1426
0.1				188.0997		188.1235
0.13				188.0997	3.6739	188.1235	6.1967
0.17				188.0884	1.4131	188.1139	4.7667
0.2				188.0658	3.3913	188.0949	5.2434
0.23				188.0545	6.2174	188.0853	5.2434
0.27				188.0149	10.174	188.0758	6.1967
0.3				187.9697	7.3479	188.0567	5.9107
0.33				187.9188	9.4957	188.0377	6.1014
0.37				187.8849	12.2088	188.0186	6.5781
0.4				187.8227	15.6566	187.99	7.4361
0.43				187.738	19.6131	187.9519	8.9614
0.47				187.6475	15.4305	187.9233	8.0081
0.5				187.6249	17.7479	187.8851	8.9614
0.53				187.6249	19.5566	187.8661	9.8194
0.57				187.6249	20.6871	187.847	10.5821
0.6				187.5967	21.2523	187.8089	11.5355
0.63				187.5571	17.2392	187.7803	10.2008
0.67				187.5175	17.974	187.7517	10.7728
0.7				187.4836	18.6523	187.704	11.5355
0.73				187.4554	19.2175	187.6754	12.2981
0.77				187.4101	19.5566	187.6659	12.8702
0.8				187.348	17.5784	187.6277	11.6308
0.83				187.2971	18.2001	187.6087	12.0121
0.87				187.218	19.0479	187.561	12.5841
0.9	digi	Both		187.1275	20.0088	187.5038	13.2515
0.93				187.0427	20.9697	187.4561	13.9188
0.97				186.958	19.6697	187.4085	12.9655
1				186.8845	20.7436	187.3417	13.6328
1.03				186.7884	21.874	187.2941	14.3955
1.07				186.698	23.1175	187.2655	14.9675
1.1				186.5962	24.361	187.2178	15.7302
1.13				186.568	22.7784	187.1701	14.6815
1.17				186.534	23.7393	187.1987	15.0628
1.2				186.4888	24.6436	187.1701	15.5395
1.23				186.3701	25.6045	187.1415	15.8255
1.27				186.2345	26.6219	187.0843	16.3022
1.3				186.1271	25.2088	187.0271	15.2535
1.33				186.0875	26.1697	186.9604	15.7302
1.37				186.0762	27.0175	186.9127	16.2069
1.4				186.0367	27.8088	186.8269	16.7789
1.43				185.9858	28.4871	186.7602	17.3509
1.47				185.9236	29.0523	186.703	17.8275
1.5				185.8727	29.5045	186.6458	18.3996
1.53				185.8275	29.9002	186.5791	18.9716
1.57				185.7427	30.2958	186.4742	19.5436
1.6				185.6862	30.6349	186.3884	20.2109
1.63				185.6297	30.9176	186.3217	20.8782
1.67				185.5449	31.2567	186.2454	21.4503
1.7				185.4601	31.5958	186.1691	22.1176
1.73				185.3584	31.9915	186.0738	22.8803
1.77				185.2284	32.6132	185.9785	23.6429
1.8				185.0758	33.4045	185.8736	24.501
1.83				184.9514	34.2524	185.7878	25.359
1.87				184.8158	35.0437	185.702	26.3123
1.9				184.6801	35.8915	185.6162	27.1703
1.93				184.5671	36.6828	185.5495	28.0283
1.97				184.4484	37.4741	185.4637	28.8863
2				184.341	38.2654	185.3397	29.8397
2.03				184.2449	39.0002	185.2634	30.6977
2.07				184.1658	39.622	185.1776	31.651
2.1				184.064	40.1872	185.0823	32.509
2.13				183.9849	40.7524	184.987	33.3671
2.17				183.8945	41.2046	184.9107	34.2251
2.2				183.7701	41.7698	184.8058	35.0831
2.23				183.6401	42.3915	184.7105	35.9411
2.27				183.4988	43.1263	184.5961	36.7991
2.3				183.3688	43.9176	184.5008	37.7524
2.33				183.3179	44.6524	184.415	38.6105
2.37				183.3575	45.1046	184.3101	39.5638
2.4				183.3688	45.4437	184.2243	40.4218
2.43				183.3236	45.6698	184.1385	41.1845
2.47				183.2388	45.8959	184.0527	41.9472
2.5				183.1314	46.0089	183.9383	42.6145
2.53				183.0127	46.122	183.8144	43.2818
2.57				182.9053	46.2916	183.7095	43.7585
2.6				182.8149	46.6307	183.6237	44.2352
2.63				182.7019	47.0263	183.5474	44.7119
2.67				182.5605	47.422	183.4616	45.0932
2.7				182.4532	47.7611	183.3568	45.3792
2.73				182.374	47.9307	183.2614	45.7605
2.77				182.2553	48.1003	183.147	46.0465
2.8				182.1592	48.1568	183.0422	46.3325
2.83				182.0971	48.1568	182.9278	46.6185
2.87				182.001	47.9872	182.8229	46.9046
2.9				181.8992	47.8742	182.7085	47.1906
2.93				181.7636	47.7046	182.5941	47.5719
2.97				181.6336	47.535	182.4892	47.8579
3				181.5149	47.3655	182.3748	48.1439
3.03				181.3905	47.2524	182.2699	48.3346
3.07				181.2605	47.1394	182.1841	48.6206
3.1				181.1305	47.1394	182.0983	48.8112
3.13				181.0175	47.2524	182.0125	49.0019
3.17				180.9101	47.422	181.9077	49.2879
3.2				180.814	47.6481	181.7742	49.4786
3.23				180.7236	47.9307	181.6789	49.6693
3.27				180.6388	48.2133	181.6026	49.8599

100

Time [min]	Event Name	Chamber	Event Text	1A: O2 concentration [µM]	1A: O2 slope neg. [pmol/(s*mL)]	1B: O2 concentration [µM]	1B: O2 slope neg. [pmol/(s*mL)]
0				190.2695		193.029
0.03				190.0227		192.5794
0.07				189.7758		192.0945
0.1				189.5341		191.5832
0.13				189.2925	122.131	191.1248	240.2283
0.17				189.0456	121.8683	190.6752	238.906
0.2				188.8145	120.5551	190.2256	232.2942
0.23				188.5833	118.9792	189.7495	228.3271
0.27				188.3207	120.2924	189.3087	227.8863
0.3				188.0686	121.5006	188.8944	231.4126
0.33				187.8269	121.6057	188.5065	227.2692
0.37				187.58	121.9209	188.0745	222.5969
0.4				187.3331	122.3936	187.6602	218.0127
0.43				187.081	122.9714	187.2723	214.1338
0.47				186.8394	122.3936	186.8844	220.3048
0.5				186.603	122.3936	186.4877	216.7785
0.53				186.3666	122.3411	186.0645	213.2522
0.57				186.1302	122.236	185.6767	209.9022
0.6				185.8781	122.236	185.3064	206.9931
0.63				185.6312	122.0784	184.9538	212.547
0.67				185.3843	122.0784	184.5835	209.3733
0.7				185.1322	122.1835	184.1868	206.2878
0.73				184.8643	122.4461	183.8342	203.2023
0.77				184.6069	122.7613	183.4992	200.2931
0.8				184.3705	122.6037	183.1465	205.4062
0.83				184.1289	122.7613	182.8027	202.497
0.87				183.8767	122.9189	182.4589	199.4997
0.9				183.6036	123.1816	182.1063	196.5905
0.93				183.3514	123.4442	181.7801	193.9458
0.97				183.1098	123.2341	181.4539	198.7944
1	digi	Both		182.8681	123.3917	181.1454	195.9734
1.03				182.6213	123.4967	180.8016	193.2405
1.07				182.3586	123.7069	180.4489	190.5958
1.1				182.1117	123.917	180.1492	188.1274
1.13				181.8701	123.6018	179.8935	192.6234
1.17				181.6179	123.7069	179.7349	189.5379
1.2				181.3816	123.8119	179.5233	186.3643
1.23				181.2082	123.7594	179.2412	183.1025
1.27				181.0349	123.4967	178.9414	180.1933
1.3				180.8353	123.024	178.6241	184.6011
1.33				180.6041	122.7088	178.3155	181.7801
1.37				180.352	122.4987	177.9893	179.1354
1.4				180.0736	122.2886	177.6808	176.4907
1.43				179.8162	122.131	177.3899	174.1104
1.47				179.564	122.0259	177.1078	171.7302
1.5				179.3172	121.9209	176.7992	169.5263
1.53				179.065	121.7633	176.4819	167.4105
1.57				178.7971	121.6582	176.1909	165.471
1.6				178.5345	121.6057	175.9177	163.6197
1.63				178.2771	121.6057	175.6356	161.8566
1.67				178.0249	121.6582	175.3358	160.0934
1.7				177.7623	121.6582	175.0361	158.5066
1.73				177.5049	121.7633	174.7363	157.0079
1.77				177.2527	121.8158	174.4542	155.5093
1.8				177.0006	121.9209	174.1633	154.1869
1.83				176.7695	121.9734	173.8724	152.9527
1.87				176.5226	122.0259	173.5815	151.8067
1.9				176.2652	122.0259	173.2906	150.8369
1.93				176.0131	122.0784	172.9908	149.9554
1.97				175.7557	122.1835	172.6999	149.0738
2				175.4878	122.2886	172.4443	148.2804
2.03				175.2094	122.4987	172.1886	147.5751
2.07				174.9572	122.7088	171.9329	146.8699
2.1				174.6998	122.9714	171.6597	146.2528
2.13				174.4319	123.2341	171.3952	145.6357
2.17				174.1588	123.5493	171.1219	145.0186
2.2				173.8961	123.8644	170.8486	144.5778
2.23				173.6387	124.2847	170.5753	144.137
2.27				173.3761	124.7049	170.302	143.7844
2.3				173.0924	125.1777	170.0464	143.4317
2.33				172.8245	125.703	169.7819	143.1673
2.37				172.5724	126.1757	169.5086	142.9028
2.4				172.315	126.701	169.2353	142.7265
2.43				172.0628	127.2263	168.9621	142.6383
2.47				171.7897	127.7516	168.724	142.462
2.5				171.5165	128.3294	168.486	141.9331
2.53				171.2486	128.9073	168.2303	141.2278
2.57				170.9807	129.3275	167.9659	140.5225
2.6				170.7128	129.6427	167.719	139.7291
2.63				170.4449	129.8528	167.481	138.9357
2.67				170.2033	129.9579	167.2254	138.2305
2.7				169.9669	130.0629	166.9873	137.437
2.73				169.7148	130.1154	166.7581	136.7318
2.77				169.4469	130.2205	166.5113	135.9384
2.8				169.1947	130.3256	166.2733	135.145
2.83				168.9373	130.3781	166.0264	134.3515
2.87				168.6904	130.3781	165.7972	133.5581
2.9				168.4488	130.3781	165.5768	132.7647
2.93				168.1914	130.3781	165.3564	131.8831
2.97				167.955	130.3256	165.1272	131.0016
3				167.7187	130.2205	164.9068	130.12
3.03				167.4875	130.1154	164.6952	129.1503
3.07				167.2354	129.9579	164.4925	128.2687
3.1				166.9832	129.8003	164.2721	127.299
3.13				166.7626	129.5902	164.0605	126.3292
3.17				166.5525	129.2224	163.8489	125.3595
3.2				166.3319	128.8022	163.6197	124.3898
3.23				166.1165	128.2769	163.3729	123.5082
3.27				165.8906	127.6991	163.1613	122.7148

100

Time [min]	Event Name	Chamber	Event Text	1A: O2 concentration [µM]	1A: O2 slope neg. [pmol/(s*mL)]	1B: O2 concentration [µM]	1B: O2 slope neg. [pmol/(s*mL)]
0				193.8814		192.913
0.03				193.7479		192.9517
0.07				193.6265		192.9857
0.1				193.4929		192.9808
0.13				193.3411	66.7803	192.976	-7.7559
0.17				193.1833	70.7264	192.976	-1.939
0.2				193.0436	73.7619	192.9808	0.7271
0.23				192.8858	75.5832	192.9857	-0.7271
0.27				192.734	75.5832	192.9663	0.4847
0.3				192.5762	72.912	192.9469	-1.2119
0.33				192.4062	74.9154	192.9469	1.0664
0.37				192.2423	76.9795	192.942	2.4237
0.4				192.0784	78.3758	192.942	2.5691
0.43				191.9205	79.165	192.9566	2.2298
0.47				191.7505	76.6759	192.9614	0.2908
0.5				191.5563	78.4365	192.942	1.2119
0.53				191.3802	80.0757	192.9081	2.0359
0.57				191.192	81.6541	192.8742	2.763
0.6				191.0038	83.1111	192.8451	3.684
0.63				190.8217	80.7435	192.8015	2.8115
0.67				190.6335	82.3219	192.7481	4.4112
0.7				190.4392	83.9003	192.7045	5.8654
0.73				190.2449	85.3574	192.6657	7.1257
0.77				190.0325	86.8144	192.6367	8.386
0.8				189.826	84.5681	192.593	6.7864
0.83				189.6318	86.0859	192.5252	8.2891
0.87				189.4193	87.6643	192.4331	9.8887
0.9				189.2068	89.1214	192.3894	11.343
0.93				189.0004	90.5177	192.3604	12.7003
0.97				188.7818	88.2107	192.3022	10.7613
1				188.5633	89.7284	192.2537	12.167
1.03				188.3326	91.2462	192.1907	13.4758
1.07				188.108	92.7032	192.1374	14.6877
1.1				187.8894	94.0995	192.0744	15.948
1.13				187.6648	91.8533	191.9774	13.9606
1.17	digi	Both		187.4341	93.3103	191.9095	15.3663
1.2				187.1852	94.7673	191.8368	16.6751
1.23				186.9545	96.1636	191.7496	17.9839
1.27				186.7056	97.6207	191.6914	19.2443
1.3				186.4688	95.3744	191.5896	17.2568
1.33				186.2321	96.7707	191.5702	18.5172
1.37				185.9649	98.2278	191.5121	19.6321
1.4				185.7525	99.6241	191.3957	20.7955
1.43				185.5157	100.899	191.2794	22.0558
1.47				185.285	102.1739	191.1533	23.3646
1.5				185.0543	103.4488	191.0419	24.6734
1.53				184.8175	104.6022	190.9352	26.0307
1.57				184.5565	105.8164	190.8577	27.3395
1.6				184.3015	106.9699	190.751	28.6483
1.63				184.0526	108.1234	190.6589	29.9571
1.67				183.7915	109.2162	190.5474	31.2659
1.7				183.5366	110.3696	190.4262	32.5747
1.73				183.2573	111.4624	190.3196	33.8835
1.77				182.9902	112.5552	190.2178	35.0469
1.8				182.717	113.6479	190.116	36.1618
1.83				182.4438	114.7407	189.9851	37.2767
1.87				182.1767	115.8335	189.8542	38.4401
1.9				181.9035	116.8655	189.7524	39.5065
1.93				181.6485	117.8976	189.6409	40.6214
1.97				181.3874	118.8689	189.5246	41.6879
2				181.1082	119.8403	189.4083	42.7543
2.03				180.8289	120.8116	189.2871	43.8207
2.07				180.5254	121.783	189.1417	44.9356
2.1				180.2461	122.8151	188.9914	46.0021
2.13				179.9729	123.7864	188.8363	47.117
2.17				179.6937	124.697	188.7005	48.2803
2.2				179.4083	125.6077	188.5648	49.4922
2.23				179.1048	126.579	188.4436	50.6556
2.27				178.8073	127.5504	188.3273	51.6735
2.3				178.5159	128.461	188.1964	52.74
2.33				178.2184	129.4324	188.051	53.7095
2.37				177.9209	130.4037	187.9201	54.679
2.4				177.6235	131.3751	187.7747	55.6
2.43				177.3321	132.2857	187.639	56.521
2.47				177.0285	133.1964	187.5129	57.3935
2.5				176.731	134.1677	187.3675	58.266
2.53				176.4214	135.0783	187.2124	59.1386
2.57				176.1239	136.0497	187.0767	60.0111
2.6				175.8265	137.021	186.9603	60.7382
2.63				175.5351	137.9317	186.8101	61.5138
2.67				175.2315	138.7816	186.6452	62.144
2.7				174.9219	139.753	186.4901	62.7257
2.73				174.6123	140.6636	186.3495	63.3558
2.77				174.3087	141.5135	186.2187	63.9375
2.8				173.9991	142.3028	186.0635	64.5677
2.83				173.6652	143.092	185.9133	65.2463
2.87				173.3435	143.8812	185.7485	65.925
2.9				173.0278	144.6704	185.5691	66.5551
2.93				172.7242	145.3989	185.3946	67.2823
2.97				172.3903	146.1882	185.2395	67.9124
3				172.0443	146.9774	185.0844	68.5911
3.03				171.7225	147.7666	184.9341	69.2212
3.07				171.3947	148.5558	184.7887	69.8029
3.1				171.0729	149.345	184.6287	70.3846
3.13				170.7451	150.1343	184.4833	70.8693
3.17				170.4233	150.9235	184.3282	71.4026
3.2				170.1076	151.652	184.1779	71.8873
3.23				169.7737	152.4412	184.0228	72.3236
3.27				169.4338	153.1697	183.8725	72.7598

100

Time [min]	Event Name	Chamber	Event Text	1A: O2 concentration [µM]	1A: O2 slope neg. [pmol/(s*mL)]	1B: O2 concentration [µM]	1B: O2 slope neg. [pmol/(s*mL)]
0				196.264		196.1098
0.03				195.8262		195.7933
0.07				195.378		195.4862
0.1				194.9299		195.1696
0.13				194.5024	220.9786	194.8436	157.8121
0.17				194.0594	220.4635	194.5459	156.8671
0.2				193.6267	218.6606	194.253	154.5046
0.23				193.194	217.3729	193.9364	152.8509
0.27				192.751	218.4031	193.6104	153.7959
0.3				192.308	219.3818	193.3033	155.5441
0.33				191.8547	219.6393	192.9725	156.0166
0.37				191.4066	219.9999	192.6559	156.5836
0.4				190.9791	220.1544	192.3961	155.4969
0.43				190.5412	220.515	192.1078	154.1266
0.47				190.1137	219.9484	191.7913	154.5991
0.5				189.6759	219.7423	191.5078	153.7959
0.53				189.2329	219.5878	191.2385	152.6619
0.57				188.8105	219.3303	190.9313	151.6224
0.6				188.3881	219.0212	190.6101	150.9609
0.63				187.9657	218.9697	190.3124	152.2839
0.67				187.5433	218.5061	190.0053	151.8114
0.7				187.1107	217.991	189.6698	151.6697
0.73				186.6986	217.4759	189.3627	151.4807
0.77				186.2762	216.9608	189.0697	151.3389
0.8				185.8384	217.4244	188.7673	152.2367
0.83	digi	Both		185.4211	216.9608	188.4649	152.0004
0.87				185.0039	216.4457	188.1673	151.8114
0.9				184.5918	215.8791	187.8885	151.5279
0.93				184.1849	215.3125	187.5956	151.1972
0.97				183.7676	215.9306	187.3026	151.7642
1				183.3607	215.3125	187.0569	151.2444
1.03				182.9538	214.6943	186.7876	150.7247
1.07				182.5778	213.9732	186.5088	150.1577
1.1				182.1966	213.2005	186.2064	149.638
1.13				181.8154	213.7672	185.904	150.2522
1.17				181.4033	212.9945	185.6016	149.827
1.2				180.9964	212.2219	185.3276	149.4017
1.23				180.6049	211.4492	185.0441	148.9765
1.27				180.2083	210.6765	184.7559	148.5512
1.3				179.8323	211.5522	184.4818	149.1655
1.33				179.4562	210.7281	184.1889	148.7402
1.37				179.0648	209.9039	183.9101	148.3622
1.4				178.6733	209.0797	183.6408	147.8898
1.43				178.2921	208.2556	183.3857	147.4173
1.47				177.9315	207.3284	183.1258	146.9448
1.5				177.5555	206.4012	182.8754	146.3778
1.53				177.1898	205.4225	182.5966	145.8108
1.57				176.8138	204.4438	182.3131	145.2911
1.6				176.448	203.4651	182.0249	144.8186
1.63				176.0823	202.4349	181.7556	144.3461
1.67				175.7115	201.4562	181.5146	143.8263
1.7				175.3354	200.4775	181.2689	143.3538
1.73				174.9749	199.4988	181.0138	142.7396
1.77				174.6246	198.5202	180.787	142.0309
1.8				174.2589	197.4899	180.5365	141.3221
1.83				173.8983	196.5113	180.3145	140.5661
1.87				173.5532	195.5326	180.0829	139.6212
1.9				173.1875	194.5539	179.8467	138.7234
1.93				172.8269	193.6267	179.6152	137.7784
1.97				172.4766	192.648	179.4215	136.739
2				172.1315	191.6693	179.2136	135.6522
2.03				171.7864	190.6906	179.0104	134.5655
2.07				171.4516	189.7119	178.8072	133.4315
2.1				171.1064	188.7332	178.5993	132.2975
2.13				170.7562	187.806	178.4009	131.0691
2.17				170.4265	186.8789	178.2072	129.8406
2.2				170.0968	185.9002	178.0182	128.5649
2.23				169.7517	185.0245	177.8292	127.1946
2.27				169.4272	184.0973	177.626	125.8244
2.3				169.113	183.1701	177.4464	124.4069
2.33				168.7885	182.2429	177.2669	122.895
2.37				168.464	181.3158	177.0968	121.2885
2.4				168.1446	180.3371	176.922	119.5875
2.43				167.8304	179.3069	176.733	117.9338
2.47				167.511	178.2767	176.5298	116.3746
2.5				167.202	177.2465	176.3692	114.7681
2.53				166.9032	176.2163	176.2274	113.1144
2.57				166.6045	175.1345	176.0573	111.4607
2.6				166.2954	174.1043	175.9061	109.807
2.63				166.0018	173.0226	175.7502	108.106
2.67				165.7082	171.8894	175.5848	106.4523
2.7				165.4249	170.7047	175.4336	104.7986
2.73				165.1261	169.623	175.2777	103.1921
2.77				164.848	168.4382	175.1171	101.5384
2.8				164.575	167.202	174.9706	99.8846
2.83				164.3071	165.9657	174.8336	98.2309
2.87				164.0238	164.678	174.6965	96.5772
2.9				163.7559	163.3902	174.5217	95.0652
2.93				163.4984	161.9994	174.3658	93.6478
2.97				163.2357	160.6087	174.2146	92.3248
3				162.9678	159.2179	174.0729	91.0018
3.03				162.7	157.8786	173.9264	89.7261
3.07				162.4424	156.4879	173.8035	88.4976
3.1				162.1952	155.0456	173.676	87.2219
3.13				161.9428	153.6033	173.52	86.0879
3.17				161.6955	152.1095	173.3641	85.0012
3.2				161.4586	150.6157	173.2271	83.9144
3.23				161.2216	149.1219	173.0854	82.9222
3.27				160.9898	147.6281	172.9483	82.0245