Fibroblasts proteomics

Selected Cell

Cell:

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Remarks combined_data_mFAO_processed combined_data_mFAO dataset1 dataset2 SCAD_peaks ETF_peaks CPT2_peaks

Remarks:
1. Data from two experiments were used: one from 2017-09-06 and one from 2019-04-16. The data from those are presented in two separate sheets named "dataset1" and "dataset2", respectively.
2. We try to optimize the ratios between the endogenous protein and the standards for each individual experiment, since we know that there might be a slight bias in the total protein determinations and mitochondrial content of each sample preparation. This choice is always a balance between the high and low abundant protein targets. Therefore the concentrations might be slightly different between the individual experiments but in this way we did try to choose the most optimal conditions for each experiment. We do not expect that these differences will have a significant effect on the quantification as we have shown previously with the assay development of the targeted proteomics panels that the amount of standard can vary much wider than the differences described within this manuscript, without affecting the quantification of the endogenous proteins significantly (this has been shown previous in supplementary Figure S2 from PMID: 27447838).
3. A colour code is used to indicate whether the sample could be quantified or not:
endogenously below limit of detection
endogenously near limit of detection (LOD)
endogenously detected
4. For measurements indicated as "endogenous near limit of detection", comments are given in the final column of the sheet.
5. The data in "combined_data_mFAO_processed" were the values used to make the personalised models. These pertained to the enzymes of the mitochondrial fatty acid oxidation (mFAO).
6. Two peptides of both ETFb and HADHb were quantified. Their concentrations were assumed equal to the average of the two peptides.
7. The concentration of the ETFb subunit - being the only ETF subunit that was quantifiable - was assumed for the whole ETF protein
8. The concentration of MTP was assumed equal to the subunit (HADHa and HADHb) with the lowest concentration, since both are necessary for a functional enzyme
9. A representative selection of peaks from the mass spectrometry is included in the sheets titled "SCAD_peaks", "ETF_peaks", and "CPT2_peaks"

Gene human	Concatamer	Description	dataset2_19	dataset2_23	dataset1_1	dataset2_1	dataset2_1r	dataset2_24	dataset1_2	dataset2_2r	dataset2_20	dataset1_3	dataset2_7	dataset2_13	dataset1_4	dataset2_3r	dataset2_9	dataset1_5	dataset2_27	dataset2_29	dataset1_6	dataset2_30	dataset2_32	dataset1_7	dataset2_4	dataset2_10	dataset1_8	dataset2_14	dataset2_25	dataset1_9	dataset2_5	dataset2_11	dataset2_15	dataset1_10	dataset2_21	dataset2_28	dataset2_12	dataset2_16	dataset1_104	dataset2_6	dataset2_31	dataset1_105	dataset2_8	dataset2_17	dataset1_106	dataset2_22	dataset2_26
			fmol/μg total protein
			MCADD patient 1			MCADD patient 2				MCADD patient 3			MCADD patient 4			MCADD patient 5			MCADD patient 6			MCADD patient 7			MCADD patient 8			MCADD patient 9			MCADD patient 10				Control C103		Control C104			Control C105			Control C106.K			Control C106.W
Endogenously above LOD
Etf	3	Connection between beta-oxidation and electron transport chain	0.4966591248415799	0.6270332968477024	0.6308369053173759	0.7077270072170817	0.7179851748434269	1.1386455197378516	1.1571348789932097	0.8147007857569635	0.3603844119067416	1.3536346926328342	1.4294967515192	1.2713941398228799	1.960606270882245	0.8869822991367886	0.53439377913665	1.9839214772741665	1.462869465575877	1.11658839298262	2.4435888762757965	1.2621898654758432	1.1881957311842883	0.7091290244427582	1.1431900954288805	1.2917895148145146	1.2873412644389788	1.0176430934656215	0.5196216160158049	1.9316165868746675	0.6680422959181432	1.111330416938918	0.5423271642146774	1.8020124258213963	0.38677481804840996	0.9082965368088851	0.8680118679109794	0.6010957836728077	1.3530399203085157	0.9930791015787472	2.036451542101254	1.5935379913204015	1.0984046139833215	0.5820926031463212	2.397554770220318	0.8418049762378237	0.47967381178174534
Acaa2	3	Fatty acid beta-oxidation	1.6734211678043451	2.581585482220506		1.6195646432048891	1.492337675939352	4.109044827983872		1.604865519390731	0.726879094966025		3.431482911182991	3.649979558937737		2.80171604521171	1.1097439963564582		4.108065532909626	3.000038122850278		4.756795888838512	3.6020979919128924		3.467239812002254	3.36818748281985		2.7913380008213737	0.9423002938861778		2.136927186838676	4.595540312554574	1.7804866299400977		1.5291722941853738	3.4463960446224084	1.9258062654501273	1.204167236976341		3.50875767654409	5.8612836068409715		3.190010391032954	1.9517249897405917		2.573998334104268	1.076481818741697
Acadm	3	Fatty acid beta-oxidation	0.15625949284655646	0.15802436520241678	0.0953148455645683	0.11139408048007547	0.11389707568477161	0.13820799577032067	0.06597836149502259	0.12016673027468881	0.16374933738763112	0.03686137143206077	0.18235826158247284	0.3178048623935429	0.09923015453797653	0.30240504589542894	0.21540207571434258	0.07220243228547285	0.2066987415720299	0.21943619174813564	0.3018963942847722	0.27385826313498673	0.067968278382826	0.05265495779890368	0.3510356777929107	0.1756682571420841	0.0752160919128832	0.4493282596364032	0.08426652169333804	0.12296416199773995	0.1441169837746733	0.35108856073883676	0.3360297399388219	0.10304198425706636	0.5710372665598672	1.7757545211460672	0.9213698138239449	0.9946921397761206	0.4338412787614099	1.4174277888357687	3.714958953492663	0.4935671239592106	1.1576331314524826	0.5380793345929047	1.1855317621615975	1.381376135464805	1.0305584964836934
Acadvl	3	Fatty acid beta-oxidation	0.4219459547370374	0.7325752326212825	1.012536681504813	0.5338552021666313	0.7309089140227764	1.5016649865312324	1.793910411404304	0.8060130152394104	0.32869247987163724	1.7443243104649815	1.6816168016114235	1.3615895110532812	2.967420966673717	1.1056073426619792	0.636645512002039	4.151407373879249	1.1135297971319702	0.6958409124189666	2.888092816181492	1.2119972384400644	0.7165722561897185	0.999713133324143	1.4378107343511226	1.421787064402906	1.8009189111004884	0.7921100583637138	0.40935261716507515	3.5739454660094885	0.8515640919354575	1.5966844451533704	0.4645197336520584	3.4738905291708346	0.43206787148209064	0.9557053920897121	0.6565955873156317	0.575207648692797	2.0537424794699675	0.9134108691785101	1.6600588806938252	2.6069944982277127	0.9781753181414268	0.5186884947096202	3.9968360174491284	0.624747057474579	0.4231318468312688
Mtp	3	Fatty acid beta-oxidation	0.951289011304325	1.411063509168786	0.38911937953092984	0.6802246778825113	0.6745386560941389	2.4519031782257166	0.5984968740030568	0.9277281400508649	0.5747809532575537	0.5118894668456103	1.7212816480133453	2.331025212630771	1.3800724775789983	0.9539672032565779	0.6830023102930132	1.3713802382859237	1.4272796689801002	1.1403277063974422	1.5669564624637233	2.0602751768606256	1.1584427410400828	0.6493228489024728	1.8666602370609242	2.7828412911075366	0.8168394756714017	1.0778530737709748	0.5630057803468209	1.8006987592435073	0.954213384198593	2.7915347902345373	0.8769898731552831	2.5939384977788222	0.6893496009133244	1.76781243885266	0.735823352280452	0.498305058019105	0.5851482738332198	1.5235982108681065	3.5988773607112465	0.7859061814886414	1.3622466009448966	0.6954558160912034	1.8588503222755504	1.2220347266709268	0.5532215080471481
Issues with quantification (see comments)
Acads	3	Fatty acid beta-oxidation	0.17521633437958264	0.14291189228054374		0.15941692131387786	0.10988861464341607	0.16362788787718935		0.15226393035636934	0.0999914640455695		0.23281865545441965	0.18741673870937234		0.246752307316136	0.18777371804211526		0.18298757018723352	0.1879736875089546		0.2843754458546575	0.12209647661656604		0.26105607593130886	0.3058432695036521		0.18368217397010023	0.11872287557374761		0.33152028952521406	0.4503150327188889	0.25926065242940893	0.46314242587824117	0.15286173070636574	0.3076768446973494	0.17601741754084146	0.12057800368890335		0.2894206268910302	0.25279833917640687		0.3204435025555802	0.18465048164224895	0.46357469960837083	0.12332096136294494	0.1270971651804887

Gene human	Concatamer	Description	Peptide	dataset2_19	dataset2_23	dataset1_1	dataset2_1	dataset2_1r	dataset2_24	dataset1_2	dataset2_2r	dataset2_20	dataset1_3	dataset2_7	dataset2_13	dataset1_4	dataset2_3r	dataset2_9	dataset1_5	dataset2_27	dataset2_29	dataset1_6	dataset2_30	dataset2_32	dataset1_7	dataset2_4	dataset2_10	dataset1_8	dataset2_14	dataset2_25	dataset1_9	dataset2_5	dataset2_11	dataset2_15	dataset1_10	dataset2_21	dataset2_28	dataset2_12	dataset2_16	dataset1_104	dataset2_6	dataset2_31	dataset1_105	dataset2_8	dataset2_17	dataset1_106	dataset2_22	dataset2_26
				fmol/μg total protein
				MCADD patient 1			MCADD patient 2				MCADD patient 3			MCADD patient 4			MCADD patient 5			MCADD patient 6			MCADD patient 7			MCADD patient 8			MCADD patient 9			MCADD patient 10				Control C103		Control C104			Control C105			Control C106.K			Control C106.W
Endogenously above LOD
Etfb_1	3	Connection between beta-oxidation and electron transport chain	Etfb_H_LGPLQVAR	0.647907282572531	0.8696759871450938	0.8955141951471414	0.9678694200372395	1.0146432230359117	1.462685935344285	1.7382078709575242	1.1173492377354155	0.4779580935140407	1.7846751859703924	2.027295533598395	1.7900679373426092	2.5440892806183353	1.2865700405620593	0.7844665623281833	2.605909973044891	1.9290863911666267	1.4681465471705784	3.064491225973723	1.687447076289019	1.6313402724645563	0.9531869667006094	1.619617852150213	1.830337831618817	1.6360180349474622	1.4350632809274289	0.7047600381415432	2.486999566309476	0.8706665968445897	1.6152555890895934	0.7797606598807695	2.1978982655348185	0.4875314454959646	1.1973560083409263	1.2376105046156805	0.79434718124113	1.8182704999350332	1.321475879840337	2.528578242387712	1.9951695786255406	1.5107833309924696	0.8155008072013562	3.034259048314396	1.1204252132827406	0.6204208102697532
Etfb_2	3	Connection between beta-oxidation and electron transport chain	Etfb_H_LSVISVEDPPQR	0.34541096711062885	0.38439060655031093	0.36615961548761033	0.44758459439692394	0.4213271266509421	0.8146051041314182	0.5760618870288953	0.5120523337785113	0.24281073029944245	0.9225941992952758	0.8316979694400054	0.7527203423031507	1.3771232611461546	0.48739455771151796	0.2843209959451166	1.3619329815034418	0.9966525399851273	0.7650302387946615	1.82268652657787	0.8369326546626676	0.7450511899040205	0.46507108218490684	0.6667623387075479	0.753241198010212	0.9386644939304956	0.6002229060038143	0.3344831938900666	1.3762336074398587	0.46541799499169684	0.6074052447882425	0.30489366854858546	1.406126586107974	0.28601819060085526	0.6192370652768439	0.49841323120627823	0.4078443861044853	0.8878093406819981	0.6646823233171575	1.5443248418147957	1.1919064040152625	0.686025896974173	0.34868439909128607	1.7608504921262405	0.5631847391929068	0.3389268132937375
Acaa2	3	Fatty acid beta-oxidation	Acaa2_H_DFTATDLSEFAAK	1.6734211678043451	2.581585482220506		1.6195646432048891	1.492337675939352	4.109044827983872		1.604865519390731	0.726879094966025		3.431482911182991	3.649979558937737		2.80171604521171	1.1097439963564582		4.108065532909626	3.000038122850278		4.756795888838512	3.6020979919128924		3.467239812002254	3.36818748281985		2.7913380008213737	0.9423002938861778		2.136927186838676	4.595540312554574	1.7804866299400977		1.5291722941853738	3.4463960446224084	1.9258062654501273	1.204167236976341		3.50875767654409	5.8612836068409715		3.190010391032954	1.9517249897405917		2.573998334104268	1.076481818741697
Acadm	3	Fatty acid beta-oxidation	Acadm_MH_ANWYFLLAR	0.15625949284655646	0.15802436520241678	0.0953148455645683	0.11139408048007547	0.11389707568477161	0.13820799577032067	0.06597836149502259	0.12016673027468881	0.16374933738763112	0.03686137143206077	0.18235826158247284	0.3178048623935429	0.09923015453797653	0.30240504589542894	0.21540207571434258	0.07220243228547285	0.2066987415720299	0.21943619174813564	0.3018963942847722	0.27385826313498673	0.067968278382826	0.05265495779890368	0.3510356777929107	0.1756682571420841	0.0752160919128832	0.4493282596364032	0.08426652169333804	0.12296416199773995	0.1441169837746733	0.35108856073883676	0.3360297399388219	0.10304198425706636	0.5710372665598672	1.7757545211460672	0.9213698138239449	0.9946921397761206	0.4338412787614099	1.4174277888357687	3.714958953492663	0.4935671239592106	1.1576331314524826	0.5380793345929047	1.1855317621615975	1.381376135464805	1.0305584964836934
Acadvl	3	Fatty acid beta-oxidation	Acadvl_MH_FFEEVNDPAK	0.4219459547370374	0.7325752326212825	1.012536681504813	0.5338552021666313	0.7309089140227764	1.5016649865312324	1.793910411404304	0.8060130152394104	0.32869247987163724	1.7443243104649815	1.6816168016114235	1.3615895110532812	2.967420966673717	1.1056073426619792	0.636645512002039	4.151407373879249	1.1135297971319702	0.6958409124189666	2.888092816181492	1.2119972384400644	0.7165722561897185	0.999713133324143	1.4378107343511226	1.421787064402906	1.8009189111004884	0.7921100583637138	0.40935261716507515	3.5739454660094885	0.8515640919354575	1.5966844451533704	0.4645197336520584	3.4738905291708346	0.43206787148209064	0.9557053920897121	0.6565955873156317	0.575207648692797	2.0537424794699675	0.9134108691785101	1.6600588806938252	2.6069944982277127	0.9781753181414268	0.5186884947096202	3.9968360174491284	0.624747057474579	0.4231318468312688
Hadha	3	Fatty acid beta-oxidation	Hadha_MH_DGPGFYTTR	1.0152444421098408	1.4021553934752786	0.7630262390442815	0.9052849847708502	0.8226113797258731	2.7500626477661365	0.6673644846790914	1.1471905876624948	0.6892900074594346	0.8227094621376665	1.7822870800286241	2.727234995074236	1.1671277123655837	1.0945842154203396	0.9260114816100499	1.5858707338483837	1.4108044872635117	1.1556639361801821	1.4435023166357603	2.0657888431116547	1.0336572007168263	1.1098694896584331	1.9253445628278028	2.985600273128842	0.7044577258504491	1.2350656536269924	0.7025582434098747	2.010570694822984	0.9744559142172937	2.770361432719466	0.9744175141000214	2.494365377145849	0.7262240191689713	1.8134801349348	0.9126094716662672	0.6373402514908555	0.7559369956571084	1.6227234637344974	2.724459393612542	0.8059486927220973	1.5080443300781832	0.7889781367501423	1.8124006575026024	1.244931175504215	0.46809682830108157
Hadhb_1	3	Fatty acid beta-oxidation	Hadhb_MH_DQLLLGPTYATPK	1.343393418614823	1.4831216899801503	1.3294563250724691	1.1686036510716113	1.2318794702679368	3.263311475025505	1.381997982667866	1.4931998282158492	0.8634796604104553	1.7649293210302446	2.4210519787472196	3.336801889411483	2.5422556635243723	1.5420177683930922	1.1259504320714027	2.786930850999267	1.8150856522922114	1.4114463658055598	3.346547876612276	2.662359990261362	1.9361802548530858	1.9455318161422297	2.677357899056783	3.3812957042421647	1.6312441158514805	1.5527971576784507	0.869025900335419	3.5568409363535927	1.5662948947499435	3.721041962418683	1.2701077016506452	5.910819682910989	0.951168108624666	2.499034149860536	1.1773998685337341	0.7007761864871549	1.426036009147415	2.474723396722039	5.264878983127289	1.8131290926650792	1.8687704522183226	0.9657490322936035	3.9159496038098935	1.4317861182004172	0.7937440189864007
Hadhb_2	3	Fatty acid beta-oxidation	Hadhb_MH_LAAAFAVSR	0.951289011304325	1.411063509168786	0.38911937953092984	0.6802246778825113	0.6745386560941389	2.4519031782257166	0.5984968740030568	0.9277281400508649	0.5747809532575537	0.5118894668456103	1.7212816480133453	2.331025212630771	1.3800724775789983	0.9539672032565779	0.6830023102930132	1.3713802382859237	1.4272796689801002	1.1403277063974422	1.5669564624637233	2.0602751768606256	1.1584427410400828	0.6493228489024728	1.8666602370609242	2.7828412911075366	0.8168394756714017	1.0778530737709748	0.5630057803468209	1.8006987592435073	0.954213384198593	2.7915347902345373	0.8769898731552831	2.5939384977788222	0.6893496009133244	1.76781243885266	0.735823352280452	0.498305058019105	0.5851482738332198	1.5235982108681065	3.5988773607112465	0.7859061814886414	1.3622466009448966	0.6954558160912034	1.8588503222755504	1.2220347266709268	0.5532215080471481
Issues with quantification (see comments)
Etfa	3	Connection between beta-oxidation and electron transport chain	Etfa_MH_TIYAGNALCTVK	0.291026447306261	0.16755686721635468	0.0835639847910982	0.1295431647195271	0.3203400115330546	0.34671267562386193	0.1829546205513756	0.38501131155971974	0.17413618509433773	0.2339499036608863	0.34144287645359495	0.47540796426440907	0.3012367571707299	0.40983091369522123	0.4735477740585926	0.2905859181679522	0.4578312194605262	0.3660720148530706	0.6872611973590768	0.5345611809095914	0.4213450639604077	0.14673749715611106	0.372174289283442	0.5297856037845631	0.2605875662415156	0.4309103176984936	0.16945764069280952	0.3102431687825536	0.3834254912268498	0.4710721458157517	0.2596432707966252	0.33101694118861036	0.2343833240218093	0.3436032487407781	0.29537472973107143	0.255559631491099	0.19302067830616113	0.2972571907244413	0.6685440843914702	0.23633393777929038	0.3198650176401871	0.26886707866301096	0.3258917949185266	0.31466389277744905	0.13628949727215622
Acads	3	Fatty acid beta-oxidation	Acads_MH_ITEIYEGTSEIQR	0.17521633437958264	0.14291189228054374	0.12097720131829617	0.15941692131387786	0.10988861464341607	0.16362788787718935	0.17443726656225278	0.15226393035636934	0.0999914640455695	0.1039104747414965	0.23281865545441965	0.18741673870937234	0.18938026745547726	0.246752307316136	0.18777371804211526	0.2735916387447427	0.18298757018723352	0.1879736875089546	0.24674189395625656	0.2843754458546575	0.12209647661656604	0.20846490988467065	0.26105607593130886	0.3058432695036521	0.3213867729878224	0.18368217397010023	0.11872287557374761	0.25197916577429	0.33152028952521406	0.4503150327188889	0.25926065242940893	0.46314242587824117	0.15286173070636574	0.3076768446973494	0.17601741754084146	0.12057800368890335	0.1649837122286186	0.2894206268910302	0.25279833917640687	0.2370955872405927	0.3204435025555802	0.18465048164224895	0.46357469960837083	0.12332096136294494	0.1270971651804887
Cpt2	3	Fatty acid beta-oxidation	Cpt2_MH_SEYNDQLTR	0.14793625257764823	0.15016914754660332	0.17642832131801667	0.19201415478111478	0.13835446423566158	0.25293820815232126	0.1533978703533935	0.1082202864532791	0.09796929976881885	0.09487999923750023	0.12912475328190146	0.17595533272216257	0.21267359466729358	0.19435779757184876	0.18547586430890814	0.33811084019810994	0.1684144573331197	0.1930102057152198	0.2766185976311226	0.2427991633820617	0.1838736322521763	0.16939526247826986	0.237507046165218	0.24940764377794072	0.14255305293490383	0.18090364221110436	0.1009835423803463	0.3451553160901835	0.13382363094602673	0.21170773589765607	0.1310083690155365	0.36528903261333895	0.1067403240391485	0.17580580206820914	0.16621087648515712	0.17404012645852937	0.22448787409728482	0.2848039063701984	0.24613804570359926	0.2421625237964631	0.20189295040057295	0.14983220368440403	0.483589223023315	0.15933867165828075	0.09366417242038404

Gene human	Concatamer	Description	dataset1_1 conc	dataset1_2 conc	dataset1_3 conc	dataset1_4 conc	dataset1_5 conc	dataset1_6 conc	dataset1_7 conc	dataset1_8 conc	dataset1_9 conc	dataset1_10 conc	dataset1_104 conc	dataset1_105 conc	dataset1_106 conc
			fmol/μg total protein
			MCADD patient 1	MCADD patient 2	MCADD patient 3	MCADD patient 4	MCADD patient 5	MCADD patient 6	MCADD patient 7	MCADD patient 8	MCADD patient 9	MCADD patient 10	Control C104	Control C105	Control C106
Endogenously above LOD
Gpx4	1	Antioxidant enzyme	0.688190018781767	1.0665621676171448	1.1366531696417401	1.386030319304213	2.8080625715481347	1.322334048501443	0.6545900096599938	1.2343533630630301	1.469867057125041	1.4791654166136416	1.6204204278965908	1.545164795610723	2.0109732865272463
Prdx6	1	Antioxidant enzyme	2.1830216647405845	5.416594989036829	6.876125065005954	9.782828925400397	11.31682826380407	17.324281038174963	3.144090975315782	10.510862758918213	13.411043701820825	6.885359742459085	8.200575439722806	6.9164662953243505	8.387242789614646
Sod2	1	Antioxidant enzyme	0.9722202653578688	1.6537719150076922	1.9820752247852969	2.6307199601629847	2.6346425418097086	1.679900358703724	1.3865121978154893	1.0384892268054258	3.8917011135021413	15.581670797883032	2.596959085178724	3.3476203297541662	2.9328999360629826
Ndufs1	1	Complex I (NADH dehydrogenase)	0.18373511866454117	0.2263180633423616	0.1339553703043958	0.3584533185389322	0.46682076542942597	0.542711919157506	0.4298148193571288	0.2909518620295445	0.5253945518643732	0.7110834785160787	0.20656393285147528	0.25771189561285934	0.51077942482687
Sdha	1	Complex II (Succinate dehydrogenase)	0.7521126331259226	0.8204812319713407	0.44994848202437787	1.6817224360510876	1.3191162506190968	2.4992753099296343	0.9954039844656166	1.0603450391065123	1.5311125322189354	1.9038557308758495	0.6156718134387661	1.228753127397375	1.2204962625972688
Uqcrc2	1	Complex III (coenzyme Q : cytochrome c oxidoreductase)	1.3315229897317498	0.9842002181177835	0.30686320478093265	0.7005404813871342	2.339967195188628	2.906443117030533	1.2590455113784305	0.8181240496485072	1.5999505301749997	1.8203526287959337	0.9353408672309328	0.7558251921800524	1.0005678999712742
Cox5a	1	Complex IV (Cytochrome c oxidase)	0.9637374372829685	0.9596252178278131	1.1784655685611276	2.252446651479775	3.825724618548375	4.409568715750599	1.1248251008568246	2.6593704984677267	3.535128982079546	2.0166420721894416	2.1447578902233992	3.153181053388445	2.641412985203406
Atp5b	1	Complex V (ATP synthase)	5.11006740141987	2.793579553751674	3.5751398944200896	4.319110964466017	6.151322035571361	11.649351268955325	4.797791642668777	2.7193780593217634	5.9966288129553345	8.51079302449491	3.6399276434054704	5.602154193675255	5.714811937358288
Cycs	1	Electron transport chain and oxidative phosphorylation	0.7172807348008651	1.6722769541084948	1.049424749950521	4.15905515207029	3.699084037080836	8.457681369599849	0.8169778550102185	2.938794584132534	4.49407430657848	2.8495785035258647	2.1020659032339455	3.6844386820251556	3.1713691720793697
Slc25a3	1	Substrate transport	4.419135049009293	3.1190445022979847	1.4232208160714803	2.5818023198691864	4.632506231015196	3.9151362873038744	3.9523413059796932	2.1301373242107986	4.323885044217607	7.82547669981304	2.5625977341684787	3.282084854078415	2.225679353276072
Slc25a4	1	Substrate transport	0.11545154338246824	0.14592811282544071	0.052148676435722366	0.0878544806173116	0.17337756215647437	0.15382202899206773	0.2725234690618201	0.14905086410106597	0.186214513141639	0.6924971849044764	0.14128245573013884	0.15580886244050765	0.07172783923093907
Slc25a5	1	Substrate transport	0.9495233520940918	0.8573939882494941	0.22296006267635188	0.5948997273083098	1.1436884496456188	2.210909572254812	0.8105750528352083	0.687154116672365	0.9945036193770458	1.5425820203419023	0.5652254576123699	0.6613763176181185	0.6222040791206065
Dlat	2	Connection between glycolysis and TCA cycle	0.45935238649039084	0.21421315976271785	0.2512354057559722	0.3746453319652176	0.6031023505689844	0.7852566747322334	0.36613166102033423	0.31084575771961037	0.6460523713926087	0.6942629255662012	0.45701485436756933	0.5658207815242553	0.47484183958134274
Dld	2	Connection between glycolysis and TCA cycle	0.6975866690992009	0.5880399084557876	0.9099627311522048	1.4779433993640994	1.94375257319808	2.2710827363711723	0.8859544988956114	1.0703709806923547	1.8738413345960516	2.2046251566187145	0.9287269726399238	1.7602273556096644	1.7853564807290219
Pdha1	2	Connection between glycolysis and TCA cycle	0.47126275069970713	0.5190221400600082	0.47599612051401435	0.7306572651489663	0.9254793264564426	1.5019029099678	0.5439221981935549	0.5793975774215074	1.231385555196955	1.1055831331039614	0.5472055803790836	0.8310432445917679	1.0035382751899566
Pdk1	2	Connection between glycolysis and TCA cycle	0.2495687550935864	0.408004359786363	0.427843931476636	0.6837130561705242	0.6713432818092077	0.48977764060984086	0.18510232970467397	0.5688083030403301	0.7150772310626657	0.708383050642186	0.36015698404036006	0.39484137096322114	0.5403525617330968
Slc25a1	2	Substrate transport	0.2935145718016661	0.40446573018469395	0.17790548098106584	0.2419412263710292	0.4280251828842846	0.5665210243361384	0.40782611022873466	0.25634882055099834	0.5081347153600487	0.9106095614336225	0.3298769364505959	0.33885369809745486	0.3337647203785117
Aco2	2	TCA cycle	1.1154233616438995	1.2763120833957098	2.328022391266346	2.998807522537887	2.656224671475452	4.355496287137658	1.3339271479186219	1.8546445628049022	3.454382138353976	3.7619586741874027	2.961836739580313	2.3641640597461073	3.5396028341269874
Cs	2	TCA cycle	3.3386862215561663	8.2317144413356	4.830019955788233	14.937222869519005	11.536953978372267	11.308140445738253	4.631849600369031	10.072622488119682	13.621820333602512	11.274019050749454	6.71245747030345	12.699629207858484	17.421186973216955
Dlst	2	TCA cycle	1.5075466658298187	0.7445824756169583	0.6239936853927497	1.0326489269222583	1.4203941419304111	3.185093067149223	1.5485810917704608	0.7813024986287959	1.6778733471199563	2.6027057836922345	1.0851849519241161	1.7601777235438387	1.6467091295116774
Fh (Fh1)	2	TCA cycle	1.0526993112557788	1.5018668719584285	1.5560841771948173	3.9309586281274815	4.058478051963109	4.425035163659502	1.1396180855809956	2.150609342781459	3.2655410870873425	4.554619847726361	1.800577129224469	2.874771652986756	4.921159227819483
Idh2	2	TCA cycle	0.9557399597352256	1.021191345019046	1.805905738021236	3.9231741697681755	4.463280097405782	5.23602870634149	1.8375573208631815	1.2513388762099054	4.674927741166636	5.246686464202148	3.2261624106617113	3.5681086079144335	5.787930511113651
Idh3a	2	TCA cycle	0.46610794296179037	0.87689127819918	0.6920022615249053	1.152979867248524	1.2424444764805447	1.5528431382876338	0.310056487996205	0.6979266203497976	1.370463983261358	1.6324666899700597	0.5547220844924898	1.1344152012274282	1.7445052958728342
Mdh2	2	TCA cycle	2.864833969703803	6.822107882344501	5.761090434272565	13.460435596577245	13.729074146952662	23.136557929375016	3.941201629767768	9.535669860644866	13.649572923128513	16.776164776847448	7.420450429790847	13.703631329768125	17.353795477321135
Ogdh/Ogdhl	2	TCA cycle	0.8148563484159137	0.5495873555242835	0.3879743239618944	0.8309791998305519	0.6966648187151119	1.5213240098284422	0.8893806511988799	0.6114098793484456	0.8715527329358863	1.2025263095939838	0.609995647032684	0.8191180975119265	0.9064334224637697
Sucla2	2	TCA cycle	0.5792181933705091	0.8248612904797477	1.1234643716790642	1.4226410769220075	1.9822991405547357	1.9363950824343783	0.7249034145507142	1.0854736281980975	1.7909422970799667	1.975110376710583	0.9770259702165017	1.5970281690951516	2.6088153526364986
Suclg1	2	TCA cycle	0.2740528558666088	0.4551336919275767	0.3815787576825922	0.8351063531936197	0.8853299117783944	1.0656980763572628	0.22767822018363984	0.6060633523808481	0.7723160071646318	1.037702479431104	0.4556941871251678	0.7597452984132164	1.2323456850515675
Suclg2	2	TCA cycle	1.2708306766764015	1.3035286904386847	1.6387673593017336	1.8636873251011528	2.488683959525143	2.3828357406351834	1.0577189141332568	1.1074838919087533	1.99204350520858	3.1058647045631864	2.1270060348245026	2.34622708275743	3.594284053586139
Etfa	3	Connection between beta-oxidation and electron transport chain	0.0835639847910982	0.1829546205513756	0.2339499036608863	0.3012367571707299	0.2905859181679522	0.6872611973590768	0.14673749715611106	0.2605875662415156	0.3102431687825536	0.33101694118861036	0.19302067830616113	0.23633393777929038	0.3258917949185266
Etfb	3	Connection between beta-oxidation and electron transport chain	0.6308369053173759	1.1571348789932097	1.3536346926328342	1.960606270882245	1.9839214772741665	2.4435888762757965	0.7091290244427582	1.2873412644389788	1.9316165868746675	1.8020124258213963	1.3530399203085157	1.5935379913204015	2.397554770220318
Etfdh	3	Connection between beta-oxidation and electron transport chain	0.15730733237407116	0.12581802117023663	0.2233890838163541	0.23729793912492428	0.2711711315247131	0.28276117863979555	0.23177903293249963	0.19199738789710755	0.238869562856068	0.5380077434338447	0.17355775677499954	0.22129643921167735	0.21146684960388407
Acadl	3	Fatty acid beta-oxidation	1.4558826352356002	2.2658124165738247	1.7058163108810587	2.165466472051361	2.6606356453964715	4.385115963173894	1.045711108824926	1.782688508066098	2.1323436060906014	2.343691134465744	2.123878795171647	2.4712957648321447	3.376265200172583
Acadm	3	Fatty acid beta-oxidation	0.0953148455645683	0.06597836149502259	0.03686137143206077	0.09923015453797653	0.07220243228547285	0.3018963942847722	0.05265495779890368	0.0752160919128832	0.12296416199773995	0.10304198425706636	0.4338412787614099	0.4935671239592106	1.1855317621615975
Acads	3	Fatty acid beta-oxidation	0.12097720131829617	0.17443726656225278	0.1039104747414965	0.18938026745547726	0.2735916387447427	0.24674189395625656	0.20846490988467065	0.3213867729878224	0.25197916577429	0.46314242587824117	0.1649837122286186	0.2370955872405927	0.46357469960837083
Acadvl	3	Fatty acid beta-oxidation	1.012536681504813	1.793910411404304	1.7443243104649815	2.967420966673717	4.151407373879249	2.888092816181492	0.999713133324143	1.8009189111004884	3.5739454660094885	3.4738905291708346	2.0537424794699675	2.6069944982277127	3.9968360174491284
Cpt2	3	Fatty acid beta-oxidation	0.17642832131801667	0.1533978703533935	0.09487999923750023	0.21267359466729358	0.33811084019810994	0.2766185976311226	0.16939526247826986	0.14255305293490383	0.3451553160901835	0.36528903261333895	0.22448787409728482	0.2421625237964631	0.483589223023315
Decr1	3	Fatty acid beta-oxidation	0.4496049112908558	1.0783840705569885	0.811435363233498	1.4453462943456663	1.6436045758191944	1.5167246151200962	0.4474507133463459	0.8348758918814667	1.574738403113376	1.6447668958126993	1.1669424404353794	1.2759615191046234	2.37888496275987
Hadh	3	Fatty acid beta-oxidation	0.1226289196243261	0.22281062694233197	0.32153413037494466	0.3153084221789219	0.2836911749417932	0.24489192168115076	0.14242857736654443	0.15756160686249784	0.29334827083686293	0.2591617559844835	0.2838712074564744	0.26204861190392387	0.2710171467788706
Hadha	3	Fatty acid beta-oxidation	0.7630262390442815	0.6673644846790914	0.8227094621376665	1.1671277123655837	1.5858707338483837	1.4435023166357603	1.1098694896584331	0.7044577258504491	2.010570694822984	2.494365377145849	0.7559369956571084	0.8059486927220973	1.8124006575026024
Hadhb	3	Fatty acid beta-oxidation	0.8592878523016995	0.9902474283354614	1.1384093939379274	1.9611640705516853	2.079155544642595	2.4567521695379995	1.2974273325223513	1.2240417957614411	2.67876984779855	4.252379090344906	1.0055921414903173	1.2995176370768604	2.887399963042722
Issues with quantification (see comments)
Slc25a11	2	Substrate transport	0.22420959189346537	0.2814067999290009	0.18341293685256224	0.24519373367494263	0.23301242636547426	0.3438542515289285	0.2162803651936758	0.22935042922695237	0.329378312007238	0.5330366655557496	0.18986586996900706	0.3669641170957431	0.15698337373254584	endogenously near LOD for subset of the measurements

Gene human	Concatamer	Description	dataset2_19	dataset2_23	dataset2_1	dataset2_1r	dataset2_24	dataset2_2r	dataset2_20	dataset2_7	dataset2_13	dataset2_3r	dataset2_9	dataset2_27	dataset2_29	dataset2_30	dataset2_32	dataset2_4	dataset2_10	dataset2_14	dataset2_25	dataset2_5	dataset2_11	dataset2_15	dataset2_21	dataset2_28	dataset2_12	dataset2_16	dataset2_6	dataset2_31	dataset2_8	dataset2_17	dataset2_22	dataset2_26
			fmol/μg total protein
			MCADD patient 1	MCADD patient 1	MCADD patient 2	MCADD patient 2	MCADD patient 2	MCADD patient 3	MCADD patient 3	MCADD patient 4	MCADD patient 4	MCADD patient 5	MCADD patient 5	MCADD patient 6	MCADD patient 6	MCADD patient 7	MCADD patient 7	MCADD patient 8	MCADD patient 8	MCADD patient 9	MCADD patient 9	MCADD patient 10	MCADD patient 10	MCADD patient 10	Control C103	Control C103	Control C104	Control C104	Control C105	Control C105	Control C106.K	Control C106.K	Control C106.W	Control C106.W
Endogenously above LOD
Gpx4	1	Antioxidant enzyme	0.5710907338311658	0.41304994539405976	1.450425885692631	1.4164567380296242	0.9232469718662237	0.7253993637105494	0.4684084227144274	0.8613226070296295	0.6668082877675658	1.1999671493839552	1.1668839229024626	0.4640994917544622	0.41473296167977924	0.5265731092076406	1.0696203602277559	0.8523529046781034	0.9900296235079612	0.6783770048008128	0.5207319607981942	0.8851550654687599	0.8674651796726938	1.0844625746309249	0.7099416292012997	0.7102315225342195	0.902095282217082	0.6488858283164224	0.524041342861204	0.7006028107948986	0.8390018169307463	0.5185065518387286	0.36354642833053047	0.43682899622315424
Gpx4	1	Antioxidant enzyme	0.5810614783875999	0.3868732035460544	0.9116574834204619	1.0370673900875198	0.6824064676927947	0.557577823640102	0.3668409797851675	0.550139776998556	0.5207394844955613	0.7900325555357623	0.7333084042677904	0.36366230472672445	0.45905714796505925	0.40353160202149824	1.0656607281110095	0.5339354613334295	0.488171777844322	0.44345999762948923	0.464663613990926	0.5740662787278065	0.5780976945811047	0.6961593450647812	0.3405348426788789	0.5213249168993499	0.7003848341463562	0.5193236505898173	0.336582550953678	0.40516971942413255	0.5768895706985016	0.3831527554161503	0.32501190837930927	0.3675178496645882
Prdx6	1	Antioxidant enzyme	5.30856386769387	3.535061626944782	15.820311841628934	17.699013158086434	9.519647703346365	16.276495662962706	10.884507598854684	11.195736490667212	12.823878816326218	9.247809821800354	9.767141958036325	8.567957846090259	7.807692223389513	8.790060902465978	11.978008944682179	13.176285649773389	10.502244561230967	10.768369097307444	8.830026483717281	7.139222582287112	5.830893752102459	8.056711787786814	2.8070345462226762	4.373183900845494	9.251657506881411	7.6519949123269795	4.7013034042672155	7.37412325437923	6.4722427162444385	4.472702454610784	4.664123853110077	7.827434736246576
Sod2	1	Antioxidant enzyme	12.012051483960855	15.964132673749088	15.433085900335865	16.395571116256182	65.39961755243597	10.83939703175342	3.234619634331937	20.550638366615786	12.21496385657668		21.49451465281334	25.254445134185413	66.34031213290822	28.382024643142287	60.958564521393285		19.948529132110394	11.839107685885061	15.570696330023026		40.860739652256356	29.17752444264878	11.252588181401743	19.415570305400816	13.101288991494483	8.095360887671378	18.551596039999254	59.882880713918865	6.861159220638922	11.397467368010911	46.60864828412206	44.82588597842836
Ndufs1	1	Complex I (NADH dehydrogenase)	0.21157473035439137	0.19699647496257666	0.22654334763610734	0.21120769555208677	0.32412947918008983	0.2737746591683243	0.2077267611527073	0.29923047980793355	0.2209685264182061	0.2808413290358759	0.29581427532913823	0.4575768484502105	0.25040636126428173	0.3178853198984713	0.18396272255117543	0.3292244698449259	0.27545200672637293	0.3223234799502383	0.22013442013218287	0.23537554787185308	0.423005401437816	0.18011740373733623	0.1550811236271496	0.35725991897311965	0.2079160246533128	0.21316433169571838	0.4591678118859289	0.4220273141644189	0.3079710691066346	0.27860216500109064	0.21057103737719213	0.09002391288369177
Sdha	1	Complex II (Succinate dehydrogenase)	0.2870030759867514	0.5208661743788653	0.39295026978404035	0.3859858372716647	0.7025860063906392	0.5677750802283897	0.241896150176827	0.800180687668103	0.7974383225930352	0.7038702694634704	0.4706505592341222	0.9613214301433888	0.6821512960577485	0.4439293633797502	0.4820762401938417	0.9333919725028456	1.035149710174014	0.6337845467101357	0.4087003283293117	0.4276223777425691	0.8748876151251147	0.46899154270533755	0.27397970247942904	0.5860978599439474	0.5216223017127036	0.41102696960397855	1.4538126016987383	1.2469045202204763	0.7751416023305592	0.4289829023286797	0.5117880314117129	0.38737141344617304
Sdhb	1	Complex II (Succinate dehydrogenase)	0.5412725397547054	0.6616654353256973	0.37426995271907143	0.39663747537594357	1.1495859354157256	0.9804871689201664	0.20364997887814057	1.0534506319788006	1.9042288793593412	0.6758513292336051	0.5110913415152114	0.947062119907856	1.052202131294972	0.7674117324028584	0.9026026678651712	1.0865932553776372	1.3502148533945708	0.7191737288135593	0.6435858844054212	0.543302113941852	1.455259314744481	1.0843700738431703	0.38003822610657845	0.5392539283226773	1.0838860797675591	0.6509006281639602	2.933439420164217	2.9263075093583573	0.9396787682164113	0.8012638110374101	0.5463343763465361	0.2328442342440704
Atp5b	1	Complex V (ATP synthase)	2.782794738117188	2.8021944721834613	3.1209709742877405	3.1553110180394373	6.122458389586872	3.1128576822224243	1.9301966807267497	3.805475241690516	5.225484501196454	3.883751986200045	3.2882977287527346	5.113356714926468	3.18235943585005	4.317981860876703	3.1690617554569966	4.478863135504693	5.852174792431397	3.540685222814843	2.371909722063683	2.5041817232845447	5.669369538296405	2.2902637076211554	1.8690058922711539	4.377973188164095	3.131040709401576	2.528566875970686	8.933892606612249	9.089347580157623	4.504257117677325	3.0942043741100864	2.0997151594299073	1.1182994727696087
Atp5b	1	Complex V (ATP synthase)	2.6440713275916634	2.897610059197498	2.306661643637507	2.6612634803249993	6.372157241129867	3.050108176029326	2.070999063587304	4.023191032961722	4.813649818217626	3.1094909321631974	2.922777541507871	5.277833452507127	3.499402101959115	4.862959817940967	3.0662196737951386	4.492908920292039	6.114204094843333	3.72275938958541	2.031816704089156	2.392385748531064	4.9955253458240625	2.0749671095866393	2.237934298325428	4.065996087325359	2.7787093004316286	2.519604504923641	7.797117452089641	9.634073856339588	4.4394897503159365	2.8432609960004527	2.330602239890294	1.627969116818359
Cycs	1	Electron transport chain and oxidative phosphorylation	2.661927080344852	1.6188924705093992	4.7867607624139135	5.366199596080595	3.8686213211025224	2.644479492403305	1.4639037089914546	3.385134126677686	4.3209540647571405	3.8009826844174435	2.0824920826446056	4.345362050848203	3.513696965561202	4.215353938185444	5.355296003473065	3.2619488150684974	4.1001139182904405	3.5229723555320023	2.6808599635492922	2.2863584414032236	2.739355032889098	2.910265127107126	1.4821008007536507	2.551945323201802	2.684943854540365	2.4215134394795417	4.147778348847492	6.356562995306865	3.48618572347386	1.8235003305348925	2.8489630074322663	2.4459495954456583
Cycs	1	Electron transport chain and oxidative phosphorylation	3.2560877591180892	1.6562363913777922	3.0188765425936213	5.42486028428202	4.66687608284555	2.6222759071061814	1.307120857608788	4.2495321815570755	3.1625424278302647	7.624693174371973	4.884557351651177	5.670176599460826	4.458451184809715	4.042273732163195	6.318221086725593	3.4842459553158704	4.597383599944872	4.651645726242849	3.636056261593946	3.100950678414889	4.17179173115114	2.2498440225979452	2.002997784484095	2.276896114671693	7.533656896180532	2.486848586966837	3.156369366621644	10.456596018043097	3.890775388465594	1.6773149499121165	3.51263703452496	4.704636906807375
Slc25a3	1	Substrate transport	1.626013896560747	2.7448778776182703	1.2421092421092423	2.2900089789493525	3.8515791696560684	4.90981605516402	0.7019705608050164	2.8157772816368456	5.5714282976488905	3.342848117529072	4.760102508447786	6.002989826426802	2.4782407054264755	3.9681739164790018	3.823529949351773	4.298112499926451	3.687979463851409	3.917025666004086	3.5528624281361787	3.0405547856779074	6.874802086992083	4.295470656816278	1.0323584687265859	3.506327070728725	2.989597737479094	2.017843460001627	13.572207476475727	7.270883943044436	2.833026078407557	3.590217500934896	2.5064657878217202	2.090811569893312
Slc25a4/Slc25a6	1	Substrate transport	0.24931150604131663	0.3651803108084956	0.35114988848534245	0.2611129743675325	0.4897072685414001	0.6307702517051997	0.1751515736954773	0.4634785435770682	0.34175682654764566	0.6407473217059007	1.0329219470998943	0.45917397348965844	0.3087929204571218	0.22392374449785488	0.4428516891489015	0.5238345623783165	0.3952502742413312	0.31364455147924947	0.21007323803720512	0.3088704032910259	0.44805233445640347	0.31130996739603867	0.10721356329319609	0.27078308656774197	0.42307484319492283	0.47324242252427645	2.2198895597493546	0.6779522112003399	0.32778885467714164	0.5985983895833994	0.3637809107471781	0.3956222023609564
Slc25a5/Slc25a6	1	Substrate transport	0.7063683939431793	0.8792857618277646	0.6544899758125698	0.7012991324945838	1.8041128368926282	1.6263435991112316	0.22834305275075611	0.9315526346502385	1.1169793871213252	1.596008500743178	2.046948780333267	2.1011657235305803	1.4755611318938655	1.3840034506817667	2.3025664571848696	1.420184854792042	1.08417133138174	1.1181587789209457	1.0270679661092852	0.8116296934850097	1.5791975970821714	1.4303250239849443	0.3543222351387896	1.1808257966548314	1.159115071495006	1.4490106234397682	6.1509080100400055	3.9849805147133504	0.8238284545778649	1.9283285148038896	1.1683262146971252	1.4773048968900169
Slc25a5	1	Substrate transport	0.20482112785690795	0.20119662624700355	0.4349670979296253	0.34571009212609044	0.3958094205403292	0.5048115634064155	0.14084660617050518	0.35874965760105076	0.189749838988689	0.5723854328459189	0.7200141645720572	0.5119517635325304	0.30412010898468933	0.2792485832158513	0.31961956220047005	0.46286691580784584	0.34436415499395406	0.32731147416721235	0.3102915359550753	0.35605749931452074	0.47796248776570943	0.3313933151318331	0.15528093672607504	0.2039233401171683	0.5285767417853917	0.45790346346304533	1.6517655470534287	0.609921255382133	0.41550195843610715	0.46674723023918124	0.2859982823812949	0.2549324715982378
Pdha1	2	Connection between glycolysis and TCA cycle	0.5135253601200755	0.5188854847655096	0.3833361219246486	0.5363590704934235	1.182351122878224	0.5202503564075618	0.3636823585504151	0.8017240467379744	0.9951400688222702	0.4927052313645438	0.5016170986673915	1.1273909102617963	0.608169679477382	0.6241932821174996	0.5093926349167106	0.7886020289920807	1.1535206592200653	0.794216502529573	0.47421201800168267	0.37345125137436547	0.8440319543065647	0.2972170605248052	0.28757409505433157	0.5262457310610152	0.6194212341150154	0.46360259011939464	1.5014782521495602	1.6613007830925306	0.801953725829488	0.4039896427680655	0.47417667020666954	0.25334183091388335
Pdk1	2	Connection between glycolysis and TCA cycle	0.2616027805352698	0.41869676315803284	0.2789531332200426	0.22821762792346947	0.48959009795818065	0.46004467085070994	0.23866121874487697	0.5697350501464187	0.6989478119452572	0.4520854966106653	0.2469307581151325	0.7981345689585678	0.6639030278155066	0.7736252267600023	0.4446831575456978	0.6676268652318651	0.5835519140266711	0.46329849625777814	0.15376959484747582	0.36714560869356416	1.134468810700074	0.27094064439267523	0.2857521919428603	0.7501202972138105	0.406446595161578	0.28527645715733585	0.4316313266781924	1.2903430983136472	0.34869854105403614	0.20468315297095335	0.39006431030583255	0.23501898135642993
Pdk1	2	Connection between glycolysis and TCA cycle	0.23572997918045843	0.5134536435559991	0.24540650659164975	0.26432206573419165	0.6571835360472605	0.5809794355266301	0.34403129675441546	0.7356902470713441	0.8980916498716424	0.6296895197860316	0.38466454148244805	1.0196889691748303	0.803186393089806	0.9903697181486678	0.6292476103421378	0.9549537275560459	0.8218124312664742	0.6029379632731003	0.29583489811441566	0.4394093231528991	1.5137720462501805	0.3290554254402678	0.301469049517024	0.9776630409368731	0.4066638942013715	0.3485731565805777	0.6155941492062288	1.668070973530545	0.4405431739328913	0.2171243284133182	0.5844477399483282	0.2667090136066768
Aco2	2	TCA cycle	1.12158901195639	1.604441884443332	1.1976400518667183	1.2705506846436383	2.8507856637166253	1.5679903977196392	0.7013069402885629	2.856650289928767	2.179244357014795	1.9576295215407458	0.9705121035303474	4.217100349578336	2.9058264548445965	3.0337906374270016	1.6267414224513308	2.6211034428395705	3.016224222643347	2.541569951198688	1.1053474424375604	1.4418288530948096	2.325490932410851	1.0682120733508522	0.887435840828661	2.056393530362818	1.648070415932683	1.1087855878849806	2.43941818913886	4.220060838671932	2.6176759177056086	1.4479826989085254	2.071002309227741	1.1242779860243046
Cs	2	TCA cycle	1.8268510548765329	2.515068799738758	2.2386194747472965	2.2482022380388105	4.3241869601915495	2.5468479323938986	1.0997813248121413	5.488505013672205	4.6355746732216305	3.6365961214606397	2.2510944966628887	7.129020024720305	5.088199196831491	5.211594442143129	4.165331493789843	5.316683731042138	5.525826823581764	4.449343511211497	1.9210722762749757	2.038648019869759	3.8048824615810566	2.055570107733323	1.2091182355163017	3.46373225888235	3.4658542893833735	2.1083577208566937	4.583977785142644	8.884351601802136	5.35118291009762	2.4111080297595757	3.8908493606517043	1.635956497900104
Dlst	2	TCA cycle	0.9296205109350791	0.7037379354819598	0.8611341397730642	0.7443780048940992	1.3663465351552178	0.6211540960755978	0.5313751766811607	0.6778259089996265	0.7033194323914574	0.8052371060459123	1.0266161771457967	1.2277722755025604	0.7724381405429268	0.8672172882481266	0.7799529808254175	0.7887526215156869	1.1154609062490424	0.6455549322993651	0.8505749746853648	0.6375916519587419	1.099946342888887	0.7186902567722019	0.5630419091024248	1.2469892384786003	0.9429424186870996	0.8179203540044061	2.6453035081794916	1.6519858353187968	0.9884510201029386	0.7560022216625731	0.6433631206504857	0.4563388214227862
Fh (Fh1)	2	TCA cycle	2.701455313763279	2.2651152549197247	2.6971901030816996	2.607130527739643	3.5875627063205986	2.40415874551542	1.647457560396876	5.5810914284723125	4.481149557536236	3.469744847118969	3.0008924662979295	5.166169206222374	4.2115004597338155	4.7057274807639615	3.5559860073423017	3.4999032259479437	4.39075159894226	4.379394565629384	2.740860304697212	2.979168334328844	4.857556960180538	2.99515975508299	1.8583888896483738	3.8599261310161785	3.0814361692274095	2.9388675143357634	4.248473441771924	8.830819332971487	5.0904940791947855	2.51917461896888	3.9947932790722493	2.8116500215587896
Idh2	2	TCA cycle	0.7448268890573668	0.7922007866452312	0.7129987129987131	0.9095306033190282	0.9644912685197046	1.4125204537310196	0.5320964704987804	3.0264180716575853	2.0493708886619553	1.877031249369273	0.9059522013847727	2.971454345486959	2.4373832020173487	3.571233180082738	1.904070393229489	1.4847937518770475	1.5268454166089358	2.067354502915686	1.1187408461092672	1.614821581931343	2.5649200227657656	1.046357662629694	0.7741465096003731	1.5352766486545208	1.5197783395553732	0.889154448361405	2.4713249221281823	3.054075344757317	3.0632690830365252	1.2430500502649957	1.9623716739995811	0.813495444517136
Idh3a	2	TCA cycle	0.666587489539173	0.6850858025316408	0.706708146256169	0.78692992681432	1.359788007365846	0.7172191184361572	0.3322645434875975	1.2366274393946857	1.4351152145159418	1.1530097508767854	0.6904614853101654	1.631444294973707	1.153401196736906	1.5139174783091698	1.057663971713032	1.1074208489497368	1.5680835352383817	1.1362672184403273	0.5982494729639917	0.7323186781251299	1.662275910550727	0.5779885009100826	0.5206284592236802	1.3692426643673556	0.7389727104747886	0.4264029224647444	1.3608441543006191	2.967415009190466	1.223348228992108	0.6579042952385101	1.152420367678326	0.5984431009150111
Mdh2	2	TCA cycle	3.2632958044896148	4.008512726988743	5.288281946739004	4.806630161199496	7.175740002962708	5.142657741764171	2.362340760171857	8.766240056606573	7.7574437385631265	8.209276094077492	4.543948595858168	12.152222527582357	8.78457201528306	8.65650240194766	9.377367036349675	8.927442291915979	8.98791889326109	8.065570653868937	3.8307831005926958	5.112394669450211	7.894531510017809	5.003649405031427	3.372453730187368	6.762750622192161	7.506189085110711	4.777932362583072	8.393887296564927	18.73403415619213	8.165888802167872	4.206937567857108	6.828287540201182	4.376750129590625
Ogdh/Ogdhl	2	TCA cycle	0.474927780500536	0.47855498124345697	0.41822479886155367	0.4193985594174469	0.8054769425231283	0.36349092366426333	0.3549198020762346	0.6404489323399064	0.5895989328018886	0.2625076021891062	0.377703095588901	0.7539291536670608	0.44969843411428967	0.5435308443057999	0.2872860373705501	0.40398745425067073	0.6583792984762522	0.3044181933803021	0.3482701624698773	0.23885168453853456	0.4387138572314244	0.25399522997652935	0.25109597018963653	0.5151913810792639	0.4186796610439456	0.3723171508672094	1.080119744894951	0.6124733042861983	0.6286429647443775	0.4322643290834146	0.32055776461882085	0.2144598187464314
Sucla2	2	TCA cycle	0.5959553361954927	0.5793883167237371	0.8737664863091459	0.6581500969165057	1.2003316896164995	0.6791177376932426	0.4241816629640599	0.5725789910371513	0.5904927927222259	0.6959491420314307	0.36481876761177867	1.4013122694657534	0.8513366181939491	1.4550905854660123	1.0304215080302372	0.8934025483482892	1.0330283672161311	0.9966036132340469	0.7165293765452805	0.6787289237248523	0.9804134858099266	0.5206581806806575	0.3886437902833811	0.7883275007281997	0.582615772751459	0.4742625066884321	0.7249631545612905	1.3365774849873988	0.9806763785572403	0.6289658043699309	0.6978213689150242	0.5882835557102478
Sucla2	2	TCA cycle	0.6280990042465701	0.7439859721519351	0.8595926711542062	0.8438515635133881	1.5879083465688102	0.7985308606445578	0.3479796718288953	1.3010058753632021	1.2570946179963178	1.2500423037972868	0.6360546540207307	1.434692822188587	1.1546529608339244	1.4842664094781692	1.1219873255789616	1.3159035695420054	1.5429665031548996	1.3552708558269937	0.7159604996042443	0.7027141721551642	1.1900942289246437	0.7846484433454012	0.55564295124961	1.0129930164581205	0.8932419319996201	0.6839676697491442	1.015835089631681	2.147469439566473	1.292330156996669	0.7063993569189743	0.8046924839007816	0.770777567829165
Suclg1	2	TCA cycle	0.37403914199294935	0.5371578141848412	0.31422014223877354	0.43871363838058264	0.7437098264497851	0.6434561131333274	0.27091662605681305	0.7293544183056635	0.8358400160520303	0.5410572764861955	0.6641451493531799	0.9833397228879106	0.4683517928669595	0.8447699199610249	0.7549587516474934	0.7616004867129619	0.7208988973087465	0.7871176617017333	0.3360608718012963	0.2967081048377744	0.5596239609199573	0.18699189288585957	0.22980413754313606	0.6013996772362915	0.3885292328942215	0.3649590212766039	0.52418936720012	1.6513637631629723	0.7868867261093411	0.3485949403875088	0.5284648349417034	0.26181509086537025
Suclg2	2	TCA cycle	0.2700751455089882	0.40955467427441733	0.3614137095793792	0.31181527794752345	0.6264307090035545	0.4339574281824296	0.15783207270785896	0.6503696931650884	0.7239179521230348	0.6273364415719522	0.3498328291157869	0.7686119138109818	0.6304626958561432	0.7002385747102372	0.535410777556534	0.6903240711458266	0.7513361738662852	0.6825321420086589	0.2859605656864943	0.36346799671857466	0.7488243430594472	0.3040920183105026	0.19450547986101324	0.48272733586403976	0.521190257109954	0.3567044127384876	0.5867610872458652	0.9012041238284441	0.7266710095593506	0.5008566801294781	0.4485123319579611	0.18408368080475412
Etfb	3	Connection between beta-oxidation and electron transport chain	0.647907282572531	0.8696759871450938	0.9678694200372395	1.0146432230359117	1.462685935344285	1.1173492377354155	0.4779580935140407	2.027295533598395	1.7900679373426092	1.2865700405620593	0.7844665623281833	1.9290863911666267	1.4681465471705784	1.687447076289019	1.6313402724645563	1.619617852150213	1.830337831618817	1.4350632809274289	0.7047600381415432	0.8706665968445897	1.6152555890895934	0.7797606598807695	0.4875314454959646	1.1973560083409263	1.2376105046156805	0.79434718124113	1.321475879840337	2.528578242387712	1.5107833309924696	0.8155008072013562	1.1204252132827406	0.6204208102697532
Etfb	3	Connection between beta-oxidation and electron transport chain	0.34541096711062885	0.38439060655031093	0.44758459439692394	0.4213271266509421	0.8146051041314182	0.5120523337785113	0.24281073029944245	0.8316979694400054	0.7527203423031507	0.48739455771151796	0.2843209959451166	0.9966525399851273	0.7650302387946615	0.8369326546626676	0.7450511899040205	0.6667623387075479	0.753241198010212	0.6002229060038143	0.3344831938900666	0.46541799499169684	0.6074052447882425	0.30489366854858546	0.28601819060085526	0.6192370652768439	0.49841323120627823	0.4078443861044853	0.6646823233171575	1.5443248418147957	0.686025896974173	0.34868439909128607	0.5631847391929068	0.3389268132937375
Acaa2	3	Fatty acid beta-oxidation	1.6734211678043451	2.581585482220506	1.6195646432048891	1.492337675939352	4.109044827983872	1.604865519390731	0.726879094966025	3.431482911182991	3.649979558937737	2.80171604521171	1.1097439963564582	4.108065532909626	3.000038122850278	4.756795888838512	3.6020979919128924	3.467239812002254	3.36818748281985	2.7913380008213737	0.9423002938861778	2.136927186838676	4.595540312554574	1.7804866299400977	1.5291722941853738	3.4463960446224084	1.9258062654501273	1.204167236976341	3.50875767654409	5.8612836068409715	3.190010391032954	1.9517249897405917	2.573998334104268	1.076481818741697
Acadm	3	Fatty acid beta-oxidation	0.15625949284655646	0.15802436520241678	0.11139408048007547	0.11389707568477161	0.13820799577032067	0.12016673027468881	0.16374933738763112	0.18235826158247284	0.3178048623935429	0.30240504589542894	0.21540207571434258	0.2066987415720299	0.21943619174813564	0.27385826313498673	0.067968278382826	0.3510356777929107	0.1756682571420841	0.4493282596364032	0.08426652169333804	0.1441169837746733	0.35108856073883676	0.3360297399388219	0.5710372665598672	1.7757545211460672	0.9213698138239449	0.9946921397761206	1.4174277888357687	3.714958953492663	1.1576331314524826	0.5380793345929047	1.381376135464805	1.0305584964836934
Acadvl	3	Fatty acid beta-oxidation	0.4219459547370374	0.7325752326212825	0.5338552021666313	0.7309089140227764	1.5016649865312324	0.8060130152394104	0.32869247987163724	1.6816168016114235	1.3615895110532812	1.1056073426619792	0.636645512002039	1.1135297971319702	0.6958409124189666	1.2119972384400644	0.7165722561897185	1.4378107343511226	1.421787064402906	0.7921100583637138	0.40935261716507515	0.8515640919354575	1.5966844451533704	0.4645197336520584	0.43206787148209064	0.9557053920897121	0.6565955873156317	0.575207648692797	0.9134108691785101	1.6600588806938252	0.9781753181414268	0.5186884947096202	0.624747057474579	0.4231318468312688
Decr1	3	Fatty acid beta-oxidation	0.19892878029052244	0.26563575744934015	0.31129716111431843	0.2695724470403266	0.44202272662575304	0.31687269094592707	0.14702101768025005	0.5714463150208372	0.5535442364840008	0.4646935083052999	0.26662006257156556	0.5161087584590802	0.38374638100791253	0.45256452171344785	0.7606839473673974	0.48123604095130335	0.5544780026396723	0.43290292168464206	0.2737494861531184	0.3115012917470433	0.5399901342493592	0.2533586228814619	0.17159874090534347	0.3527167977053527	0.3748717474811986	0.20849935100733813	0.47758197184616985	0.8704274427252343	0.48579345037765964	0.27655863519133883	0.3664758311660526	0.17826252803968923
Decr1	3	Fatty acid beta-oxidation	0.41786321564179646	0.4753284434370494	0.6473282566465541	0.5821516589883902	0.7825123192010007	0.6329818476987019	0.32672911968821744	1.1935099965675027	1.0618723241989985	1.0782638546737968	0.5946719736334248	1.058410531407787	0.9136342406678956	0.9545536168951637	0.9257036383490387	1.1451424229470752	1.2007420271346338	1.0849007574528389	0.5210736941420746	0.710479322350478	1.2339196396329026	0.7144597732132536	0.4057479192645387	0.6893436779526675	0.8210204751344261	0.5496948273948291	0.9656290718232093	2.06587959392871	0.9889150707463836	0.49196492892916854	0.7299599795224402	0.512834020166739
Hadha	3	Fatty acid beta-oxidation	1.0152444421098408	1.4021553934752786	0.9052849847708502	0.8226113797258731	2.7500626477661365	1.1471905876624948	0.6892900074594346	1.7822870800286241	2.727234995074236	1.0945842154203396	0.9260114816100499	1.4108044872635117	1.1556639361801821	2.0657888431116547	1.0336572007168263	1.9253445628278028	2.985600273128842	1.2350656536269924	0.7025582434098747	0.9744559142172937	2.770361432719466	0.9744175141000214	0.7262240191689713	1.8134801349348	0.9126094716662672	0.6373402514908555	1.6227234637344974	2.724459393612542	1.5080443300781832	0.7889781367501423	1.244931175504215	0.46809682830108157
Hadhb	3	Fatty acid beta-oxidation	1.343393418614823	1.4831216899801503	1.1686036510716113	1.2318794702679368	3.263311475025505	1.4931998282158492	0.8634796604104553	2.4210519787472196	3.336801889411483	1.5420177683930922	1.1259504320714027	1.8150856522922114	1.4114463658055598	2.662359990261362	1.9361802548530858	2.677357899056783	3.3812957042421647	1.5527971576784507	0.869025900335419	1.5662948947499435	3.721041962418683	1.2701077016506452	0.951168108624666	2.499034149860536	1.1773998685337341	0.7007761864871549	2.474723396722039	5.264878983127289	1.8687704522183226	0.9657490322936035	1.4317861182004172	0.7937440189864007
Hadhb	3	Fatty acid beta-oxidation	0.951289011304325	1.411063509168786	0.6802246778825113	0.6745386560941389	2.4519031782257166	0.9277281400508649	0.5747809532575537	1.7212816480133453	2.331025212630771	0.9539672032565779	0.6830023102930132	1.4272796689801002	1.1403277063974422	2.0602751768606256	1.1584427410400828	1.8666602370609242	2.7828412911075366	1.0778530737709748	0.5630057803468209	0.954213384198593	2.7915347902345373	0.8769898731552831	0.6893496009133244	1.76781243885266	0.735823352280452	0.498305058019105	1.5235982108681065	3.5988773607112465	1.3622466009448966	0.6954558160912034	1.2220347266709268	0.5532215080471481
Issues with quantification (see comments)																																			Comment
Gsr	1	Antioxidant enzyme	8.992780326046853	4.690517767212849	10.032671425373202	9.087516159655006	7.480598853624643	7.300004747661777	6.843969566735942	6.741425515776459	4.745964135614243	17.673720617644168	15.956386380308235	9.325681067650626	9.693940520984782	13.280900381858379	18.21527614099475	4.340352383787743	7.366322169244209	7.627678748423238	8.199613152804643	12.390594317157682	11.520835866486514	14.078728384782004	8.803766256116836	12.647864626830778	6.47841704300807	6.39923729401704	7.093861614963219	12.654628160162948	8.972573397883675	3.5094687751462033	9.171334147354754	9.68682751893156	risk M-oxidation, affects accurate quantification
Dld	2	Connection between glycolysis and TCA cycle	0.6082492531842396	0.6800572683791011			1.1567245426642845	1.0244378572523907	0.6299383420507852		0.9727896956321103			1.9999973999974	1.230014066209944	1.5457717618235614	1.2578171243188374			1.257137093821394	0.9316986581743106			0.7656433987213704	0.5837852121320329	0.9812932287125836	0.5780208402146847	0.6940963449034223		3.0789651436280194		0.7986508703524734	1.277859112349014	0.7224601655420365	possibly interfered
Slc25a1	2	Substrate transport	0.17901214206682542	0.1994108075653843	0.32198532292963367	0.3129972347753795	0.37918136143555803	0.441138216666996	0.13127893309326633	0.28026078324309656	0.6176460970407579	0.58892535066414	0.5224692559346026	0.3665547848649562	0.24191967402299505	0.27357915238901104	0.4201450096139182	0.3249962121899535	0.535965787046673	0.45724019412795736	0.19514243434852843	0.22212995987686826	0.6315074730546314	0.395353297061077	0.17692653812746192	0.25600834122460986	0.5687572885388362	0.36035401666061917	1.1459637473798514	0.4976121231641737	0.2695096019082609	0.4074445369034548	0.21370444064580363	0.30899231829977314	endogenous above LOD only in subset of the samples
Etfa	3	Connection between beta-oxidation and electron transport chain	0.291026447306261	0.16755686721635468	0.1295431647195271	0.3203400115330546	0.34671267562386193	0.38501131155971974	0.17413618509433773	0.34144287645359495	0.47540796426440907	0.40983091369522123	0.4735477740585926	0.4578312194605262	0.3660720148530706	0.5345611809095914	0.4213450639604077	0.372174289283442	0.5297856037845631	0.4309103176984936	0.16945764069280952	0.3834254912268498	0.4710721458157517	0.2596432707966252	0.2343833240218093	0.3436032487407781	0.29537472973107143	0.255559631491099	0.2972571907244413	0.6685440843914702	0.3198650176401871	0.26886707866301096	0.31466389277744905	0.13628949727215622	endogenous near LOD, not sure if present; would have to repeat measurements with less standards for accurate quantification
Acads	3	Fatty acid beta-oxidation	0.17521633437958264	0.14291189228054374	0.15941692131387786	0.10988861464341607	0.16362788787718935	0.15226393035636934	0.0999914640455695	0.23281865545441965	0.18741673870937234	0.246752307316136	0.18777371804211526	0.18298757018723352	0.1879736875089546	0.2843754458546575	0.12209647661656604	0.26105607593130886	0.3058432695036521	0.18368217397010023	0.11872287557374761	0.33152028952521406	0.4503150327188889	0.25926065242940893	0.15286173070636574	0.3076768446973494	0.17601741754084146	0.12057800368890335	0.2894206268910302	0.25279833917640687	0.3204435025555802	0.18465048164224895	0.12332096136294494	0.1270971651804887	endogenous near LOD, not sure if present; would have to repeat measurements with less standards for accurate quantification
Cpt2	3	Fatty acid beta-oxidation	0.14793625257764823	0.15016914754660332	0.19201415478111478	0.13835446423566158	0.25293820815232126	0.1082202864532791	0.09796929976881885	0.12912475328190146	0.17595533272216257	0.19435779757184876	0.18547586430890814	0.1684144573331197	0.1930102057152198	0.2427991633820617	0.1838736322521763	0.237507046165218	0.24940764377794072	0.18090364221110436	0.1009835423803463	0.13382363094602673	0.21170773589765607	0.1310083690155365	0.1067403240391485	0.17580580206820914	0.16621087648515712	0.17404012645852937	0.2848039063701984	0.24613804570359926	0.20189295040057295	0.14983220368440403	0.15933867165828075	0.09366417242038404	endogenous near LOD, not sure if present; would have to repeat measurements with less standards for accurate quantification