Selected Cell
Cell:
Value:
InvestigationInformation
StudyInformation
AssayInformation
AssayConditions
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| #Template info | |||||||||
| #TemplateVersion | LEI-MIHCSME_userTemplate_v3 | version 3 is updated version including recommendations by users, plus ISA and REMBI compliant. | |||||||
| #DateTemplateGenerated | Jan-20-2023 | ||||||||
| #CreatedBy | RH | ||||||||
| Annotation_groups | Key | Value | Comments_User | Description_Examples_Values | |||||
| DataOwner | First Name | Heriche | First Name | ||||||
| DataOwner | Middle Name(s) | Middle Name(s) if any | |||||||
| DataOwner | Last Name | Jean-Karim | Last Name | ||||||
| DataOwner | User name | NA | Institutional user name | ||||||
| DataOwner | Institute | EMBL | Institute level, eg. Universiteit Leiden, Faculty of Science, Institute of Biology | ||||||
| DataOwner | E-Mail Address | heriche@embl.de | Institution email address | ||||||
| DataOwner | ORCID investigator | https://orcid.org/0000-0001-6867-9425 | orcid id as url, eg. https://orcid.org/0000-0002-3704-3675 | ||||||
| DataCollaborator | ORCID Data Collaborator | ORCID is of collaborator with experimental, collection or analysis part of this investigation | |||||||
| DataCollaborator | ORCID Data Collaborator | ORCID is of collaborator with experimental, collection or analysis part of this investigation | |||||||
| InvestigationInformation | Project ID | EU/NWO/consortium ID – Examples: EuTOX | |||||||
| InvestigationInformation | Investigation Title | heriche-condensation | High level concept to link related studies. Could be the main hypothesis/question you want to investigate as part of your research. Could be a chapter/thesis of your work. | ||||||
| InvestigationInformation | Investigation internal ID | NA | Corresponding internal ID for your investigation. | ||||||
| InvestigationInformation | Investigation description | ----- | Short description for your investigation. | ||||||
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| Annotation_groups | Key | Value | Comments_User | Description_Examples_Values | |||||
| Study | Study Title | Focused mitotic chromsome condensaton screen using HeLa cells | Manuscript/chapter/publication/paragraph title describing purpose or intention for one or multiple assays (screens/experiments) | ||||||
| Study | Study internal ID | NA | Study ID, linked to ELN or lab journal | ||||||
| Study | Study Description | The advent of genome-wide RNA interference (RNAi) based screens puts us in the position to identify genes for all functions human cells carry out. However, for many functions, assay complexity and cost make genome-scale knockdown experiments impossible. Methods to predict genes required for cell functions are therefore needed to focus RNAi screens from the whole genome on the most likely candidates. Although different bioinformatics tools for gene function prediction exist, they lack experimental validation and are therefore rarely used by experimentalists. To address this, we developed an effective computational gene selection strategy that represents public data about genes as graphs and then analyzes these graphs using kernels on graph nodes to predict functional relationships. To demonstrate its performance, we predicted human genes required for a poorly understood cellular function 'mitotic chromosome condensation' and experimentally validated the top 100 candidates with a focused RNAi screen by automated microscopy. Quantitative analysis of the images demonstrated that the candidates were indeed strongly enriched in condensation genes, including the discovery of several new factors. By combining bioinformatics prediction with experimental validation, our study shows that kernels on graph nodes are powerful tools to integrate public biological data and predict genes involved in cellular functions of interest. | Description of study with additional unstructured information about this study possibly not covered elsewhere in this template. | ||||||
| Study | Study Key Words | high content screen | List of key words associated with your study (EFO-terms). Examples: FDA approved drugs, Kinase inhibitors, DNA damage response, Unfolded protein response, Oxidative stress response, Inflammatory signaling, chemical similarity | ||||||
| Biosample | Biosample Taxon | NCBITAXON:9606 | NCBI-taxon id, human = NCBITAXON:9606; | ||||||
| Biosample | Biosample description | HeLa cells | Description of biosample genotype; Additional unstructured information about organisms used in this experiment. | ||||||
| Biosample | Biosample Organism | Homo sapiens | Which organism is your cell lines or tissue from. Examples: Human or mouse | ||||||
| Biosample | Number of cell lines used | 1 | In case multiple cell lines are used indicate here | ||||||
| Library | Library File Name | idr0002-screenA-library.txt | Library file info | ||||||
| Library | Library File Format | tab-delimited text | Library file info | ||||||
| Library | Library Type | siRNA librar | Library file info | ||||||
| Library | Library Manufacturer | Ambion | Library file info | ||||||
| Library | Library Version | NA | Library file info | ||||||
| Library | Library Experimental Conditions | NA | Any experimental conditions some cells were grown under as part of the study enter the information here e.g. different environmental stress conditions, or mutant background compared to wild type. | ||||||
| Library | Quality Control Description | NA | A brief description of the kind of quality control measures that were taken. | ||||||
| Protocols | HCS library protocol | NA | Url/doi protocols.io or ELN associated url. At least SOP/protocol filename | ||||||
| Protocols | growth protocol | growth protocol | We seeded 4000 HeLa cells stably expressing HIST1H2BJ-mCherry and LMNA-eGFP in each well and incubated them for 17 h at 37 C and 5% CO2. After 17 h of incubation, the medium was replaced by preheated CO2-independent imaging medium (Invitrogen; containing 10% heat-inactivated fetal calf serum, 2 mM glutamine, 100 U/ml penicillin, and 100 _g/ml streptomycin). Gas exchange was prevented by sealing the plates with Baysilone paste (Bayer, Leverkusen, Germany). Plates were then kept at least 1 h in the preheated incubation chamber of the microscope before imaging. A parameter-free gene function prediction method using kernels on graph nodes was used to select candidate genes for a focused RNAi screen. Ensembl 61 (February 2011) was used for the inference of siRNA target genes. We prepared siRNA-coated 96-well plates as described previously (Neumann et al., 2010) Images were acquired with an automated epifluorescence microscope (IX-81; Olympus-Europe, Hamburg, Germany) with a 20_ objective and a time interval of 8.5 min for 44 h. Four independent replicates were acquired for each siRNA treatment. Images were processed using the CellCognition software (Held et al., 2010). Only the H2B-mCherry channel was used. After segmentation, a training set was assembled by manually assigning _1800 nuclei from negative control wells to one of the following 10 classes based on the morphological aspect of the nuclei: interphase, early prophase, late prophase, prometaphase, metaphase, early anaphase, late anaphase, apoptosis, artifact, and out of focus. Early prophase was defined as the first visually detectable changes in the texture of chromatin after interphase, and late prophase was defined as the first appearance of clearly condensed chromatin before prometaphase. This late-prophase definition corresponds to events preceding complete disassembly of the nuclear envelope as judged by the LMNA-eGFP marker. All nuclei were then automatically assigned by a multiclass support vector machine to one of these classes. Mitoses were detected as any transition from interphase to one or more of prophase, prometaphase, or metaphase followed by at least one of either metaphase or anaphase. Mitotic nuclei were then tracked for 22 frames (eight before the detected transition and 14 after). Mitoses with too-dark nuclei or for which the first eight frames before the transition were not classified as interphase (e.g., out-of-focus nuclei) were discarded. Wells with fewer than five valid mitoses were discarded. Lack of condensin function, which results in absence of condensation in prophase, translated in our screen as a shorter prophase because the prophase class definition is based on the visual appearance of condensation before nuclear envelope breakdown. In negative control cells (i.e., treated with nontargeting siRNAs), the mode of duration of prophase was two frames (17 min). To get an overview of the screen, we derived a short prophase score for each gene as the median of the difference in the fraction of shorter prophase between all replicates involving the gene and corresponding negative controls (i.e., nontargeting siRNA). Similarly, we defined a long-prophase score by looking at the fraction of mitoses with prophase lasting >17 min. Then for each well, we tested whether the fraction of mitoses with prophase less (respectively more) than two frames was significantly different from the corresponding plate's pooled negative controls (Fisher's exact test with p < 0.1). A siRNA was then considered to affect mitotic chromosome condensation if it produced the same significant change in prophase duration in at least two replicates. A combined p value over replicates was calculated using the QFAST algorithm (Bailey and Gribskov, 1998), and siRNAs with combined p > 0.05 were not considered as hits. | Url/doi protocols.io or ELN associated url. At least SOP/protocol filename | |||||
| Protocols | treatment protocol | HCS library protocol | Url/doi protocols.io or ELN associated url. At least SOP/protocol filename | ||||||
| Protocols | HCS data analysis protocol | [HCS image acquistion and feature extraction protocol, HCS data analysis protocol] | Url/doi protocols.io or ELN associated url. At least SOP/protocol filename | ||||||
| Plate | Plate type | Example: uclear | |||||||
| Plate | Plate type Manufacturer | Example: Greiner Bio-One | |||||||
| Plate | Plate type Catalog number | Example:781081 | |||||||
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| Annotation_groups | Key | Value | Comments_User | Description_Examples_Values | |||||
| Assay | Assay Title | screenA | Screen name | ||||||
| Assay | Assay internal ID | NA | Experimental ID, e.g. aMV-010, linked to ELN or labjournal | ||||||
| Assay | Assay Description | Screen of 100 candidate genes predicted to be involved in mitotic chromosome condensation. Knock down of 22 genes resulted in signficantly shorter prophases and knock down of 18 genes caused signficant delays in prophase. | Description of screen plus additional unstructured information about screen not covered elsewhere. Stages of HCS: either primary screen, secondary screen or validation screen. EFO terms | ||||||
| Assay | Assay number of biological replicates | 4 | Total number of biol. Repl. | ||||||
| Assay | Number of plates | 12 | Total number of plates, n-plates | ||||||
| Assay | Assay Technology Type | fluorescence microscopy | Imaging method, Fbbi terms. | ||||||
| Assay | Assay Type | high content screen of cells treated with library of siRNAs | List of options: sub types of HCS e.g. Gene Knock down, RNAi, compount, EFO terms | ||||||
| Assay | Assay External URL | http://idr.openmicroscopy.org/webclient/?show=screen-102 | ELN or any other external url link to this screen. | ||||||
| Assay | Assay data URL | http://idr.openmicroscopy.org/webclient/?show=screen-102 | OMERO url link to this screen. | ||||||
| AssayComponent | Imaging protocol | HCS image acquistion and feature extraction protocol | Url to protocols.io or protocols in ELN, at least protocol file name or free text description of imaging protocol | ||||||
| AssayComponent | Sample preparation protocol | HCS image acquistion and feature extraction protocol | Sample preparation method (Formaldahude (PFA) fixed tissue, Live cells, unfixed tissue). FBbi terms | ||||||
| Biosample | Cell lines storage location | NA | Storage location according to Database used or at least location e.g. Example -150 liquid N2 tank 4th floor GE4.xx | ||||||
| Biosample | Cell lines clone number | NA | Storage location DB info | ||||||
| Biosample | Cell lines Passage number | NA | Passage number of your cells. In case different passage number is used for the replicates, please indicate it in Comment_users. | ||||||
| ImageData | Image number of pixelsX | 1344 | Indicate number of pixels in x in images | ||||||
| ImageData | Image number of pixelsY | 1024 | Indicate number of pixels in y in images | ||||||
| ImageData | Image number of z-stacks | 1 | Indicate number z stacks in image, single image is z=1 | ||||||
| ImageData | Image number of channels | 2 | Indicate number of channels in your image, single channel image c=1, including your TD channel | ||||||
| ImageData | Image number of timepoints | 330 | Images were taken in each well every 8.5 mins for 44 hours. The image folders and file names include information about the wells and time points. | Indicate number of time point(s) in your image | |||||
| ImageData | Image sites per well | 1 | Number of fields, numeric value | ||||||
| ImageAcquisition | Microscope id | Olympus IX-81 | Url to micrometa app file link or other url describing your microscope. At least Microscope name. | ||||||
| Specimen | Channel Transmission id | NA | Channel id is dependent on different machines, first or last. If No transmission is acquired state NA | ||||||
| Specimen | Channel 1 visualization method | mCherryFP | Visualization method (example: Hoechst staining ) | ||||||
| Specimen | Channel 1 entity | H2BC11 | HIST1H2BJ | Entity visualized (example: DNA ) | |||||
| Specimen | Channel 1 label | H2B-mCherry | Label used for entity (example:Nuclei) Best practice to use similar label as your CP-pipeline | ||||||
| Specimen | Channel 1 id | 1 | sequential id of channel order in your image | ||||||
| Specimen | Channel 2 visualization method | EGFP | Visualization method (example: GFP ) | ||||||
| Specimen | Channel 2 entity | LMNA | Entity visualized (example: MAP1LC3B ) | ||||||
| Specimen | Channel 2 label | LMNA-eGFP | Label used for entity (example:GFP-LC3) Best practice to use similar label as your CP-pipeline | ||||||
| Specimen | Channel 2 id | 2 | sequential id of channel order in your image | ||||||
| Specimen | Channel 3 visualization method | Visualization method (example: PI staining ) | |||||||
| Specimen | Channel 3 entity | Entity visualized (example: Death cell DNA ) | |||||||
| Specimen | Channel 3 label | Label used for entity (example:PI) Best practice to use similar label as your CP-pipeline | |||||||
| Specimen | Channel 3 id | sequential id of channel order in your image | |||||||
| Specimen | Channel 4 visualization method | Visualization method (example: Apoptotic marker Annexin V staining ) | |||||||
| Specimen | Channel 4 entity | Entity visualized (example: Apoptotic cells ) | |||||||
| Specimen | Channel 4 label | Label used for entity (example:Annexin-V) Best practice to use similar label as your CP-pipeline | |||||||
| Specimen | Channel 4 id | sequential id of channel order in your image |
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| #List of each well and plate containing entity, dose and other characteristics required for your study. | ||||||||||||||||||||
| #Examples of other characteristics might be but not limited to, library.cas, geneIDs, control.binary(0,1), cloneID, PassageNumber | ||||||||||||||||||||
| #Please, do not insert any empty row without '#' above the table below. For additional info or comments please use a Comment line indicated by a '#'. | ||||||||||||||||||||
| # | ||||||||||||||||||||
| Plate | Well | Treatment | Dose | DoseUnit | CellLine | TimeTreatment | ReplID | remarks | Sense Sequence | Antisense Sequence | Reagent Design Gene Annotation Build | Gene Identifier | Gene Symbol | Gene Symbol Synonyms | Gene Annotation Comments | Analysis Gene Annotation Build | Control Type | Control Comments | Quality Control | Channels |
| plate1_1_013 | A1 | s2748 | 50 | nmol | HeLa | 440 | 1 | CGAAAUGACUAUUACCUGATT | UCAGGUAAUAGUCAUUUCGGA | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000117399 | CDC20 | GRCh37, Ensembl release 61, Feb 2011 | positive control | early mitotic phenotype | fail | H2B- mCherry/Cy3:chromatin | |||
| plate1_1_013 | A2 | s20068 | 50 | nmol | HeLa | 440 | 1 | CGGAAGCAGUUCCAACUUUtt | AAAGUUGGAACUGCUUCCGtt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000105127 | AKAP8 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | A3 | s5681 | 50 | nmol | HeLa | 440 | 1 | CUAUUAGCCUUGGUUCUCAtt | UGAGAACCAAGGCUAAUAGga | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000164404 | GDF9 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | A4 | s15534 | 50 | nmol | HeLa | 440 | 1 | GCAGGUUUCUCAUCGAUUUtt | AAAUCGAUGAGAAACCUGCca | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000083168 | MYST3 | KATE6A | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | ||||
| plate1_1_013 | A5 | s10143 | 50 | nmol | HeLa | 440 | 1 | GGUGUUGAGUUUCUGCAAAtt | UUUGCAGAAACUCAACACCag | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000131165 | CHMP1A | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | A6 | s465 | 50 | nmol | HeLa | 440 | 1 | GAAUCUUUACAGGACUAUAtt | UAUAGUCCUGUAAAGAUUCca | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000170312 | CDK1 | CDC2 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | ||||
| plate1_1_013 | A7 | s15532 | 50 | nmol | HeLa | 440 | 1 | GAAGGAUAUUUAGAGCGGAtt | UCCGCUCUAAAUAUCCUUCtt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000083168 | MYST3 | KATE6A | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | ||||
| plate1_1_013 | A8 | s34513 | 50 | nmol | HeLa | 440 | 1 | GGUGGUCUAUAAACGUGAAtt | UUCACGUUUAUAGACCACCat | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000109805 | NCAPG | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | A9 | s19235 | 50 | nmol | HeLa | 440 | 1 | GCAUGUGCAUUUUGCUAGAtt | UCUAGCAAAAUGCACAUGCtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000010292 | NCAPD2 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | A10 | s21758 | 50 | nmol | HeLa | 440 | 1 | CGUCGAUGCUGUGCAUUGAtt | UCAAUGCACAGCAUCGACGgt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000112941 | PAPD7 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | A11 | s9131 | 50 | nmol | HeLa | 440 | 1 | ACAGCCCACUGGUCCUCAAtt | UUGAGGACCAGUGGGCUGUga | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000136997 | MYC | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | A12 | s31393 | 50 | nmol | HeLa | 440 | 1 | GCAGUGUCCUGAGUACAGAtt | UCUGUACUCAGGACACUGCcg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000072609 | CHFR | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | B1 | s1709 | 50 | nmol | HeLa | 440 | 1 | GCAAUUGUCAUAAAACCAAtt | UUGGUUUUAUGACAAUUGCtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000149311 | ATM | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | B2 | s152 | 50 | nmol | HeLa | 440 | 1 | GCAGAAGUGGGAGCGAAAAtt | UUUUCGCUCCCACUUCUGCtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000150687 | PRSS23 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | B3 | s4221 | 50 | nmol | HeLa | 440 | 1 | GCUCGUCUCCUAUCGAAAAtt | UUUUCGAUAGGAGACGAGCtt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000088305 | DNMT3B | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | B4 | s11211 | 50 | nmol | HeLa | 440 | 1 | AAGAAAAAGUCGCCCGAAAtt | UUUCGGGCGACUUUUUCUUaa | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000122304 | PRM2 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | B5 | s7424 | 50 | nmol | HeLa | 440 | 1 | AGUCCUUUAUUAAGCGCAAtt | UUGCGCUUAAUAAAGGACUtc | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000149503 | INCENP | GRCh37, Ensembl release 61, Feb 2011 | positive control | late mitotic phenotype | fail | H2B- mCherry/Cy3:chromatin | |||
| plate1_1_013 | B6 | s19398 | 50 | nmol | HeLa | 440 | 1 | CCAGACUUCCUAGUCCUUUtt | AAAGGACUAGGAAGUCUGGga | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000100918 | REC8 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | B7 | s2908 | 50 | nmol | HeLa | 440 | 1 | GAGUUACUCUCUUCCCAAAtt | UUUGGGAAGAGAGUAACUCgg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000115163 | CENPA | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | B8 | s23885 | 50 | nmol | HeLa | 440 | 1 | ACCUGGUUCUUGCAAAAGAtt | UCUUUUGCAAGAACCAGGUca | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000094916 | CBX5 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | B9 | s16187 | 50 | nmol | HeLa | 440 | 1 | GGAUCGAGGACAUGAGGAAtt | UUCCUCAUGUCCUCGAUCCtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000073009 | IKBKG | GRCh37, Ensembl release 61, Feb 2011 | fail | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | B10 | s11209 | 50 | nmol | HeLa | 440 | 1 | GUCUCACUAUAGGCGCAGAtt | UCUGCGCCUAUAGUGAGACtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000122304 | PRM2 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | B11 | s444246 | 50 | nmol | HeLa | 440 | 1 | UACGACCGGUCUAUCGUAGtt | CUACGAUAGACCGGUCGUAtt | GRCh37, Ensembl release 61, Feb 2011 | GRCh37, Ensembl release 61, Feb 2011 | negative control | non-targetting siRNA | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | B12 | s25158 | 50 | nmol | HeLa | 440 | 1 | CAAGCUCUCUAGUGACGAUtt | AUCGUCACUAGAGAGCUUGtt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000204104 | TRAF3IP1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | C1 | s444246 | 50 | nmol | HeLa | 440 | 1 | UACGACCGGUCUAUCGUAGtt | CUACGAUAGACCGGUCGUAtt | GRCh37, Ensembl release 61, Feb 2011 | GRCh37, Ensembl release 61, Feb 2011 | negative control | non-targetting siRNA | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | C2 | s11769 | 50 | nmol | HeLa | 440 | 1 | GGAUAUUAAGGACAGGAAAtt | UUUCCUGUCCUUAAUAUCCac | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000132341 | RAN | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | C3 | s25865 | 50 | nmol | HeLa | 440 | 1 | GCGUGGAAAAUAUUCGUGAtt | UCACGAAUAUUUUCCACGCca | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000077935 | SMC1B | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | C4 | s195332 | 50 | nmol | HeLa | 440 | 1 | CCACGAAUCCUCCAUGAAAtt | UUUCAUGGAGGAUUCGUGGcc | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000178257 | PRM3 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | C5 | s27674 | 50 | nmol | HeLa | 440 | 1 | GAAUUAACUUCUACAAGAAtt | UUCUUGUAGAAGUUAAUUCtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000137804 | NUSAP1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | C6 | s20794 | 50 | nmol | HeLa | 440 | 1 | CUAUCACUCUGGAACUGGAtt | UCCAGUUCCAGAGUGAUAGct | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000136824 | SMC2 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | C7 | s29693 | 50 | nmol | HeLa | 440 | 1 | GGAGGAGUCUGGAGACUAAtt | UUAGUCUCCAGACUCCUCCtt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000146918 | NCAPG2 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | C8 | s15751 | 50 | nmol | HeLa | 440 | 1 | GCUCGUAACUUCCUCGUUUtt | AAACGAGGAAGUUACGAGCtt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000072501 | SMC1A | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | C9 | s12890 | 50 | nmol | HeLa | 440 | 1 | AACAAUCUGUGACUAUUAAtt | UUAAUAGUCACAGAUUGUUtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000113558 | SKP1A | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | C10 | s196071 | 50 | nmol | HeLa | 440 | 1 | AGAGAAGUGUGGAGACGGAtt | UCCGUCUCCACACUUCUCUgg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000187166 | H1FNT | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | C11 | s26185 | 50 | nmol | HeLa | 440 | 1 | GCAGCUCAUCGACAUCAAAtt | UUUGAUGUCGAUGAGCUGCtc | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000110107 | PRPF19 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | C12 | s25157 | 50 | nmol | HeLa | 440 | 1 | GAAGCUAAUAUUAACUCAAtt | UUGAGUUAAUAUUAGCUUCtt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000204104 | TRAF3IP1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | D1 | s37878 | 50 | nmol | HeLa | 440 | 1 | CGACCAAAGAUGUGAGAAUtt | AUUCUCACAUCUUUGGUCGct | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000085415 | SEH1L | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | D2 | s51519 | 50 | nmol | HeLa | 440 | 1 | AGCUCAAGGUCAAGAGGCAtt | UGCCUCUUGACCUUGAGCUtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000188662 | HILS1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | D3 | s51520 | 50 | nmol | HeLa | 440 | 1 | CGAAAUGCCUAUCACUUCAtt | UGAAGUGAUAGGCAUUUCGgg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000188662 | HILS1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | D4 | s25212 | 50 | nmol | HeLa | 440 | 1 | GCAGGUCCUCUAUACCGCAtt | UGCGGUAUAGAGGACCUGCat | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000164880 | INTS1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | D5 | s2380 | 50 | nmol | HeLa | 440 | 1 | GCAUUUCACAAGACCUCCAtt | UGGAGGUCUUGUGAAAUGCtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000042493 | CAPG | GRCh37, Ensembl release 61, Feb 2011 | fail | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | D6 | s11338 | 50 | nmol | HeLa | 440 | 1 | GGAUGUUAGUAAACUCUCUtt | AGAGAGUUUACUAACAUCCat | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000041357 | PSMA4 | GRCh37, Ensembl release 61, Feb 2011 | fail | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | D7 | s195331 | 50 | nmol | HeLa | 440 | 1 | GCCUGUGUGAGUCAGGAUAtt | UAUCCUGACUCACACAGGCca | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000178257 | PRM3 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | D8 | s11746 | 50 | nmol | HeLa | 440 | 1 | UGAAGUGGAUUUAACUAAAtt | UUUAGUUAAAUCCACUUCAag | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000002016 | RAD52 | GRCh37, Ensembl release 61, Feb 2011 | fail | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | D9 | s43430 | 50 | nmol | HeLa | 440 | 1 | GGAAGUAAACAGUCCACUAtt | UAGUGGACUGUUUACUUCCcg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000137413 | TAF8 | GRCh37, Ensembl release 61, Feb 2011 | fail | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | D10 | s224705 | 50 | nmol | HeLa | 440 | 1 | AAGGGACAAUGAUAGUAGAtt | UCUACUAUCAUUGUCCCUUtc | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000162367 | TAL1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | D11 | s39570 | 50 | nmol | HeLa | 440 | 1 | CAGAAUAUCCUGAGAAAGAtt | UCUUUCUCAGGAUAUUCUGtt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000042286 | AIFM2 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | D12 | s14496 | 50 | nmol | HeLa | 440 | 1 | GCGGCUCUCUGUUGACUAUtt | AUAGUCAACAGAGAGCCGCtc | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000127824 | TUBA4A | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | E1 | s27673 | 50 | nmol | HeLa | 440 | 1 | GGUUUUCAGCUGCUACUAAtt | UUAGUAGCAGCUGAAAACCtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000137804 | NUSAP1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | E2 | s31701 | 50 | nmol | HeLa | 440 | 1 | CAAACUCCAUAAUACGGUAtt | UACCGUAUUAUGGAGUUUGga | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000116539 | ASH1L | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | E3 | s34567 | 50 | nmol | HeLa | 440 | 1 | CAAUUACCUUGAAGAAGUUtt | AACUUCUUCAAGGUAAUUGct | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000181191 | PJA1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | E4 | s11747 | 50 | nmol | HeLa | 440 | 1 | GACUACCUGAGAUCACUAAtt | UUAGUGAUCUCAGGUAGUCtt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000002016 | RAD52 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | E5 | s8180 | 50 | nmol | HeLa | 440 | 1 | GCUCGCAUCUAAACACCUUtt | AAGGUGUUUAGAUGCGAGCtt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000174405 | LIG4 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | E6 | s444246 | 50 | nmol | HeLa | 440 | 1 | UACGACCGGUCUAUCGUAGtt | CUACGAUAGACCGGUCGUAtt | GRCh37, Ensembl release 61, Feb 2011 | GRCh37, Ensembl release 61, Feb 2011 | negative control | non-targetting siRNA | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | E7 | s2083 | 50 | nmol | HeLa | 440 | 1 | CAGUUGAAAUUAAACGGAAtt | UUCCGUUUAAUUUCAACUGta | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000139618 | BRCA2 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | E8 | s19892 | 50 | nmol | HeLa | 440 | 1 | AGCUUAAGGCGGAUGAAGAtt | UCUUCAUCCGCCUUAAGCUgg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000102898 | NUTF2 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | E9 | s14308 | 50 | nmol | HeLa | 440 | 1 | GAGUCACAAUUGAUCCGGAtt | UCCGGAUCAAUUGUGACUCta | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000131747 | TOP2A | Q71UN1_HUMAN | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | ||||
| plate1_1_013 | E10 | s20867 | 50 | nmol | HeLa | 440 | 1 | GAGCUUCUGUUGAACGGCAtt | UGCCGUUCAACAGAAGCUCct | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000186141 | POLR3C | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | E11 | s11738 | 50 | nmol | HeLa | 440 | 1 | AAGAUAUGCUGGUACAUCUtt | AGAUGUACCAGCAUAUCUUgg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000108384 | RAD51C | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | E12 | s223193 | 50 | nmol | HeLa | 440 | 1 | GGCUUUUAGCCUGAAGAAAtt | UUUCUUCAGGCUAAAAGCCaa | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000138738 | PRDM5 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | F1 | s43431 | 50 | nmol | HeLa | 440 | 1 | CGUCUGAACUGGAGAUGCAtt | UGCAUCUCCAGUUCAGACGga | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000137413 | TAF8 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | F2 | s2744 | 50 | nmol | HeLa | 440 | 1 | GCAUUGAACAAAGCUAAAAtt | UUUUAGCUUUGUUCAAUGCcc | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000094804 | CDC6 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | F3 | s21308 | 50 | nmol | HeLa | 440 | 1 | CCAGAUUGAUCGACCAGCAtt | UGCUGGUCGAUCAAUCUGGat | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000183207 | RUVBL2 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | F4 | s21583 | 50 | nmol | HeLa | 440 | 1 | GCCUUGAAGCAUUACGACAtt | UGUCGUAAUGCUUCAAGGCtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000168439 | STIP1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | F5 | s14700 | 50 | nmol | HeLa | 440 | 1 | GCAGAACUGUAGUCUGGAAtt | UUCCAGACUACAGUUCUGCac | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000010256 | UQCRC1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | F6 | s6323 | 50 | nmol | HeLa | 440 | 1 | GGCUCACGGAAUUUGAAGAtt | UCUUCAAAUUCCGUGAGCCtc | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000185024 | BRF1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | F7 | s10773 | 50 | nmol | HeLa | 440 | 1 | CUUCAUGCAUUUUACGAAAtt | UUUCGUAAAAUGCAUGAAGca | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000101868 | POLA1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | F8 | s21759 | 50 | nmol | HeLa | 440 | 1 | CACUGAUCAUGACAACCAAtt | UUGGUUGUCAUGAUCAGUGtt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000112941 | PAPD7 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | F9 | s21582 | 50 | nmol | HeLa | 440 | 1 | CGCCUUAUCCUGGAACAGAtt | UCUGUUCCAGGAUAAGGCGca | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000168439 | STIP1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | F10 | s11207 | 50 | nmol | HeLa | 440 | 1 | GGAACAAUGCCGCCUGUCAtt | UGACAGGCGGCAUUGUUCCtt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000175646 | PRM1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | F11 | s224915 | 50 | nmol | HeLa | 440 | 1 | GCAAUCAUUUGAAACUCCAtt | UGGAGUUUCAAAUGAUUGCtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000196954 | CASP4 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | F12 | s41425 | 50 | nmol | HeLa | 440 | 1 | CCGAGAAACAGAAACGUAAtt | UUACGUUUCUGUUUCUCGGgt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000146670 | CDCA5 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | G1 | s20350 | 50 | nmol | HeLa | 440 | 1 | GGAGGAUACUUUAGAACAAtt | UUGUUCUAAAGUAUCCUCCaa | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000080986 | NDC80 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | G2 | s31700 | 50 | nmol | HeLa | 440 | 1 | GGAUUGAUAAAGAGGUAAAtt | UUUACCUCUUUAUCAAUCCct | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000116539 | ASH1L | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | G3 | s7424 | 50 | nmol | HeLa | 440 | 1 | AGUCCUUUAUUAAGCGCAAtt | UUGCGCUUAAUAAAGGACUtc | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000149503 | INCENP | GRCh37, Ensembl release 61, Feb 2011 | positive control | late mitotic phenotype | fail | H2B- mCherry/Cy3:chromatin | |||
| plate1_1_013 | G4 | s2906 | 50 | nmol | HeLa | 440 | 1 | GACUUUAGUUUGUGAGUUAtt | UAACUCACAAACUAAAGUCaa | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000115163 | CENPA | GRCh37, Ensembl release 61, Feb 2011 | fail | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | G5 | s22911 | 50 | nmol | HeLa | 440 | 1 | CGGUACUACCAGCUCGUUUtt | AAACGAGCUGGUAGUACCGgg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000083642 | PDS5B | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | G6 | s23883 | 50 | nmol | HeLa | 440 | 1 | GGAGCACAAUACUUGGGAAtt | UUCCCAAGUAUUGUGCUCCtc | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000094916 | CBX5 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | G7 | s2748 | 50 | nmol | HeLa | 440 | 1 | CGAAAUGACUAUUACCUGATT | UCAGGUAAUAGUCAUUUCGGA | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000117399 | CDC20 | GRCh37, Ensembl release 61, Feb 2011 | positive control | early mitotic phenotype | fail | H2B- mCherry/Cy3:chromatin | |||
| plate1_1_013 | G8 | s34602 | 50 | nmol | HeLa | 440 | 1 | GGAUCGAGAGUGUAAUUAAtt | UUAAUUACACUCUCGAUCCtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000121274 | PAPD5 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | G9 | s14304 | 50 | nmol | HeLa | 440 | 1 | GGAUGAUCUUUUUGAUAGAtt | UCUAUCAAAAAGAUCAUCCtc | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000198900 | TOP1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | G10 | s444246 | 50 | nmol | HeLa | 440 | 1 | UACGACCGGUCUAUCGUAGtt | CUACGAUAGACCGGUCGUAtt | GRCh37, Ensembl release 61, Feb 2011 | GRCh37, Ensembl release 61, Feb 2011 | negative control | non-targetting siRNA | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | G11 | s16186 | 50 | nmol | HeLa | 440 | 1 | AAACAGGAGGUGAUCGAUAtt | UAUCGAUCACCUCCUGUUUgg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000073009 | IKBKG | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | G12 | s22007 | 50 | nmol | HeLa | 440 | 1 | CCUUCAUUGAGGACCUGAAtt | UUCAGGUCCUCAAUGAAGGtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000184489 | PTP4A3 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | H1 | s41424 | 50 | nmol | HeLa | 440 | 1 | GCAGGGAGCUUACUAAGGAtt | UCCUUAGUAAGCUCCCUGCca | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000146670 | CDCA5 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | H2 | s19030 | 50 | nmol | HeLa | 440 | 1 | GCACUGUAGCAUACCUGUAtt | UACAGGUAUGCUACAGUGCag | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000101003 | GINS1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | H3 | s20866 | 50 | nmol | HeLa | 440 | 1 | GGACUAUGCUGAAGUAUCAtt | UGAUACUUCAGCAUAGUCCat | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000186141 | POLR3C | GRCh37, Ensembl release 61, Feb 2011 | fail | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | H4 | s21307 | 50 | nmol | HeLa | 440 | 1 | GGAGAUCCGUGAUGUAACAtt | UGUUACAUCACGGAUCUCCgg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000183207 | RUVBL2 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | H5 | s37699 | 50 | nmol | HeLa | 440 | 1 | GUACUUGAGUGACUCACUUtt | AAGUGAGUCACUCAAGUACag | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000101447 | FAM83D | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | H6 | s12836 | 50 | nmol | HeLa | 440 | 1 | CGCCCAUUCUUCAAUGUCAtt | UGACAUUGAAGAAUGGGCGgt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000196470 | SIAH1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | H7 | s6412 | 50 | nmol | HeLa | 440 | 1 | GCACCUAGAUACCAGCACAtt | UGUGCUGGUAUCUAGGUGCtt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000188486 | H2AFX | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | H8 | s15754 | 50 | nmol | HeLa | 440 | 1 | CAGAUGAAAUACUACUUCUtt | AGAAGUAGUAUUUCAUCUGgt | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000102030 | NAA10 | ARD1A | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | ||||
| plate1_1_013 | H9 | s17997 | 50 | nmol | HeLa | 440 | 1 | GGAGCUAUUCUUGAAGUUUtt | AAACUUCAAGAAUAGCUCCtg | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000183291 | SEP15_HUMAN | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | H10 | s791 | 50 | nmol | HeLa | 440 | 1 | GGUAGACUGUCAUCGUGAAtt | UUCACGAUGACAGUCUACCaa | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000113522 | RAD50 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin | |||||
| plate1_1_013 | H11 | s524 | 50 | nmol | HeLa | 440 | 1 | CCAGUACCAAAGUUGAUAAtt | UUAUCAACUUUGGUACUGGta | GRCh37, Ensembl release 61, Feb 2011 | ENSG00000139687 | RB1 | GRCh37, Ensembl release 61, Feb 2011 | pass | H2B- mCherry/Cy3:chromatin |
