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InvestigationInformation
StudyInformation
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| #Template info | |||||||||
| #TemplateVersion | LEI-MIHCSME_userTemplate_v3 | version 3 is updated version including recommendations by users, plus ISA and REMBI compliant. | |||||||
| #DateTemplateGenerated | Jan-20-2023 | ||||||||
| #CreatedBy | RH | ||||||||
| Annotation_groups | Key | Value | Comments_User | Description_Examples_Values | |||||
| DataOwner | First Name | Lukas | First Name | ||||||
| DataOwner | Middle Name(s) | Surya | Middle Name(s) if any | ||||||
| DataOwner | Last Name | Wijaya | Last Name | ||||||
| DataOwner | User name | wijayals | Institutional user name | ||||||
| DataOwner | Institute | LACDR | Institute level, eg. Universiteit Leiden, Faculty of Science, Institute of Biology | ||||||
| DataOwner | E-Mail Address | l.s.wijaya@lacdr.leidenuniv.nl | Institution email address | ||||||
| DataOwner | ORCID investigator | https://orcid.org/0000-0003-0646-9267 | orcid id as url, eg. https://orcid.org/0000-0002-3704-3675 | ||||||
| DataCollaborator | ORCID Data Collaborator | ORCID is of collaborator with experimental, collection or analysis part of this investigation | |||||||
| DataCollaborator | ORCID Data Collaborator | ORCID is of collaborator with experimental, collection or analysis part of this investigation | |||||||
| InvestigationInformation | Project ID | SysBioTop_TransQST | EU/NWO/consortium ID – Examples: EuTOX | ||||||
| InvestigationInformation | Investigation Title | Integration of temporal single cell cellular stress response activity with Logic-ODE modeling for drug-induced liver injury | High level concept to link related studies. Could be the main hypothesis/question you want to investigate as part of your research. Could be a chapter/thesis of your work. | ||||||
| InvestigationInformation | Investigation internal ID | iLW101 | Corresponding internal ID for your investigation. | ||||||
| InvestigationInformation | Investigation description | Integration of temporal single cell cellular stress response activity with Logic-ODE modeling for drug-induced liver injury. | Short description for your investigation. | ||||||
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| # comments: | |||||||||
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| Annotation_groups | Key | Value | Comments_User | Description_Examples_Values | |||||
| Study | Study Title | Temporal single cell cellular stress response activity toward toxicants inducing adaptive stress responses | Manuscript/chapter/publication/paragraph title describing purpose or intention for one or multiple assays (screens/experiments) | ||||||
| Study | Study internal ID | sLW101 | Study ID, linked to ELN or lab journal | ||||||
| Study | Study Description | Time lapse imaging (for 60 to 72 hours) of 13 reporter cell lines directly after exposure to toxicants. This study contains 13 assays, one assay for each reporter cell line, exposed to multiple compounds inducing adaptive stress responses. The expression of GFP-tagged proteins representing the investigated stress responses were followed using confocal microscopy study for 60-72 hours. The expression of the protein was quantified based on the GFP signals captured as images using high content image analysis pipeline. | Description of study with additional unstructured information about this study possibly not covered elsewhere in this template. | ||||||
| Study | Study Key Words | [HepG2, DILI, Reporter cell line, GFP] | List of key words associated with your study (EFO-terms). Examples: FDA approved drugs, Kinase inhibitors, DNA damage response, Unfolded protein response, Oxidative stress response, Inflammatory signaling, chemical similarity | ||||||
| Biosample | Biosample Taxon | NCBITAXON:9606 | NCBI-taxon id, human = NCBITAXON:9606; | ||||||
| Biosample | Biosample description | human liver carcinoma | Description of biosample genotype; Additional unstructured information about organisms used in this experiment. | ||||||
| Biosample | Biosample Organism | Human | Which organism is your cell lines or tissue from. Examples: Human or mouse | ||||||
| Biosample | Number of cell lines used | 13 | In case multiple cell lines are used indicate here | ||||||
| Library | Library File Name | SysBioTop_Compound_Library.xlsx | Library file info | ||||||
| Library | Library File Format | .xlsx | Library file info | ||||||
| Library | Library Type | All chemicals were purchased from Sigma-Aldrich – The Netherlands; except for cisplatin (Ebewe – The Netherlands) and nefazodone (Sequoia Research Products – Pangbourne, United Kingdom). All compounds were dissolved in DMSO; except for mitomycin-C (DMSO-PBS), N-acetylcysteine (PBS) and for cisplatin which was already manufactured as a solution. TNFα was purchased from R&D System (Abingdon, United Kingdom). All compounds in DMSO were maintained as 500-fold stock such that the final exposure did not exceed 0.2% v/v DMSO. | Library file info | ||||||
| Library | Library Manufacturer | Library file info | |||||||
| Library | Library Version | Library file info | |||||||
| Library | Library Experimental Conditions | Any experimental conditions some cells were grown under as part of the study enter the information here e.g. different environmental stress conditions, or mutant background compared to wild type. | |||||||
| Library | Quality Control Description | A brief description of the kind of quality control measures that were taken. | |||||||
| Protocols | HCS library protocol | SysBioTop_Screenin_Protocol.docx | Url/doi protocols.io or ELN associated url. At least SOP/protocol filename | ||||||
| Protocols | growth protocol | SysBioTop_Screenin_Protocol.docx | Url/doi protocols.io or ELN associated url. At least SOP/protocol filename | ||||||
| Protocols | treatment protocol | SysBioTop_Screenin_Protocol.docx | Url/doi protocols.io or ELN associated url. At least SOP/protocol filename | ||||||
| Protocols | HCS data analysis protocol | SysBioTop_Screenin_Protocol.docx | Url/doi protocols.io or ELN associated url. At least SOP/protocol filename | ||||||
| Plate | Plate type | 384 imaging plate uclear | Example: uclear | ||||||
| Plate | Plate type Manufacturer | Greiner Bio-One | Example: Greiner Bio-One | ||||||
| Plate | Plate type Catalog number | 781081 | Example:781081 | ||||||
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| Annotation_groups | Key | Value | Comments_User | Description_Examples_Values | |||||
| Assay | Assay Title | HepG2 A20 GFP | Screen name | ||||||
| Assay | Assay internal ID | aLW113 | Experimental ID, e.g. aMV-010, linked to ELN or labjournal | ||||||
| Assay | Assay Description | The screened was done in 384 well plate without randomization. Each plate contained only one cell line. | Description of screen plus additional unstructured information about screen not covered elsewhere. Stages of HCS: either primary screen, secondary screen or validation screen. EFO terms | ||||||
| Assay | Assay number of biological replicates | 3 | Total number of biol. Repl. | ||||||
| Assay | Number of plates | 3 | Total number of plates, n-plates | ||||||
| Assay | Assay Technology Type | confocal microscopy | Imaging method, Fbbi terms. | ||||||
| Assay | Assay Type | high content screen of cells in treated with a compound library | List of options: sub types of HCS e.g. Gene Knock down, RNAi, compount, EFO terms | ||||||
| Assay | Assay External URL | https://www.ebi.ac.uk/biostudies/eu-toxrisk/studies/S-TOXR1741 | ELN or any other external url link to this screen. | ||||||
| Assay | Assay data URL | T:\CellObservatory\LACDR\DDS\DDS_2\Bob van de Water_SysBioTop_TQ | OMERO url link to this screen. | ||||||
| AssayComponent | Imaging protocol | The plates were imaged under the confocal microscope. The plates were imaged once every 1.5 hours. The laser were used at 406 and 488 for nuclear staining and GFP expression respectively. The imaging was performed between 60-72 hours | Url to protocols.io or protocols in ELN, at least protocol file name or free text description of imaging protocol | ||||||
| AssayComponent | Sample preparation protocol | Live cells | Sample preparation method (Formaldahude (PFA) fixed tissue, Live cells, unfixed tissue). FBbi terms | ||||||
| Biosample | Cell lines storage location | Freezer -80 - tower D4 | mother stock | Storage location according to Database used or at least location e.g. Example -150 liquid N2 tank 4th floor GE4.xx | |||||
| Biosample | Cell lines clone number | clone 21 | Different clone numbers used in this study. For P53:Clone 21, for MDM2:Clone 22, for NRF2:Clone 112, for P21:Clone 4, for HMOX1:Clone 11, for ICAM1:Clone 9, for BIP:Clone 2, for XBP1:Clone 12, for ATF4:Clone 50, for CHOP:Clone 7, for BTG2:Clone 7, for SRXN1:Clone 20, and for A20:Clone 21. | Storage location DB info | |||||
| Biosample | Cell lines Passage number | 15-25 | Passage number of your cells. In case different passage number is used for the replicates, please indicate it in Comment_users. | ||||||
| ImageData | Image number of pixelsX | 512 | Indicate number of pixels in x in images | ||||||
| ImageData | Image number of pixelsY | 512 | Indicate number of pixels in y in images | ||||||
| ImageData | Image number of z-stacks | 1 | Indicate number z stacks in image, single image is z=1 | ||||||
| ImageData | Image number of channels | 2 | Indicate number of channels in your image, single channel image c=1, including your TD channel | ||||||
| ImageData | Image number of timepoints | 40 | Indicate number of time point(s) in your image | ||||||
| ImageData | Image sites per well | 2 | Number of fields, numeric value | ||||||
| ImageAcquisition | Microscope id | Nikon2 | Url to micrometa app file link or other url describing your microscope. At least Microscope name. | ||||||
| Specimen | Channel Transmission id | NA | Channel id is dependent on different machines, first or last. If No transmission is acquired state NA | ||||||
| Specimen | Channel 1 visualization method | Hoechst 33258 | Visualization method (example: Hoechst staining ) | ||||||
| Specimen | Channel 1 entity | DNA | Entity visualized (example: DNA ) | ||||||
| Specimen | Channel 1 label | Hoechst 33258 | Label used for entity (example:Nuclei) Best practice to use similar label as your CP-pipeline | ||||||
| Specimen | Channel 1 id | 1 | sequential id of channel order in your image | ||||||
| Specimen | Channel 2 visualization method | EGFP | Visualization method (example: GFP ) | ||||||
| Specimen | Channel 2 entity | TNFAIP3 | Entity visualized (example: MAP1LC3B ) | ||||||
| Specimen | Channel 2 label | A20 | Label used for entity (example:GFP-LC3) Best practice to use similar label as your CP-pipeline | ||||||
| Specimen | Channel 2 id | 2 | sequential id of channel order in your image | ||||||
| Specimen | Channel 3 visualization method | Visualization method (example: PI staining ) | |||||||
| Specimen | Channel 3 entity | Entity visualized (example: Death cell DNA ) | |||||||
| Specimen | Channel 3 label | Label used for entity (example:PI) Best practice to use similar label as your CP-pipeline | |||||||
| Specimen | Channel 3 id | sequential id of channel order in your image | |||||||
| Specimen | Channel 4 visualization method | Visualization method (example: Apoptotic marker Annexin V staining ) | |||||||
| Specimen | Channel 4 entity | Entity visualized (example: Apoptotic cells ) | |||||||
| Specimen | Channel 4 label | Label used for entity (example:Annexin-V) Best practice to use similar label as your CP-pipeline | |||||||
| Specimen | Channel 4 id | sequential id of channel order in your image |
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| #List of each well and plate containing entity, dose and other characteristics required for your study. | |||||||||
| #Examples of other characteristics might be but not limited to, library.cas, geneIDs, control.binary(0,1), cloneID, PassageNumber | |||||||||
| #Please, do not insert any empty row without '#' above the table below. For additional info or comments please use a Comment line indicated by a '#'. | |||||||||
| # | |||||||||
| Plate | Well | Treatment | Dose | DoseUnit | CellLine | TimeTreatment | ReplID | FileNames | Remarks |
| 20180824_SBP-TQ_A20_rep1 | B02 | VPA | 12137.72 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B03 | VPA | 9710.17 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B04 | VPA | 7282.63 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B05 | VPA | 4855.09 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B06 | VPA | 2427.54 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B07 | VPA | 1213.77 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B08 | VPA | 606.89 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B09 | VPA | 121.38 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B10 | BUS | 1.62 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B11 | BUS | 1.3 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B12 | BUS | 0.97 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B13 | BUS | 0.65 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B14 | BUS | 0.32 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B15 | BUS | 0.16 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B16 | BUS | 0.08 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B17 | BUS | 0.02 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B18 | CDDP | 50 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B19 | ETO | 100 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B20 | THAP | 120 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B21 | THAP | 1.2 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B22 | THAP | 0.005 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | B23 | DMEM | 100 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C02 | DIC | 1009.98 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C03 | DIC | 807.98 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C04 | DIC | 605.99 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C05 | DIC | 403.99 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C06 | DIC | 202 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C07 | DIC | 101 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C08 | DIC | 50.5 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C09 | DIC | 10.1 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C10 | MEL | 258.31 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C11 | MEL | 206.65 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C12 | MEL | 154.99 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C13 | MEL | 103.32 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C14 | MEL | 51.66 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C15 | MEL | 25.83 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C16 | MEL | 12.92 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C17 | MEL | 2.58 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C18 | BFA | 100 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C19 | BFA | 0.19 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C20 | BFA | 0.019 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C21 | TUN | 100 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C22 | TUN | 19.2 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | C23 | TUN | 1.2 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D02 | PCM | 6947.32 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D03 | PCM | 5557.86 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D04 | PCM | 4168.39 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D05 | PCM | 2778.93 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D06 | PCM | 1389.46 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D07 | PCM | 694.73 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D08 | PCM | 347.37 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D09 | PCM | 69.47 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D10 | NAC A. | 10000 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D11 | NAC A. | 8000 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D12 | NAC A. | 6000 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D13 | NAC A. | 4000 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D14 | NAC A. | 2000 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D15 | NAC A. | 1000 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D16 | NAC A. | 500 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D17 | NAC A. | 100 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D18 | CDDP | 25 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D19 | ETO | 50 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D20 | THAP | 96 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D21 | THAP | 1 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D22 | THAP | 0.001 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | D23 | DMEM | 100 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E02 | CIP | 328.94 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E03 | CIP | 263.15 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E04 | CIP | 197.37 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E05 | CIP | 131.58 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E06 | CIP | 65.79 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E07 | CIP | 32.89 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E08 | CIP | 16.45 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E09 | CIP | 3.29 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E10 | ASC | 6995.23 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E11 | ASC | 5596.18 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E12 | ASC | 4197.14 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E13 | ASC | 2798.09 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E14 | ASC | 1399.05 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E15 | ASC | 699.52 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E16 | ASC | 349.76 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E17 | ASC | 69.95 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E18 | BFA | 10 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E19 | BFA | 0.14 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E20 | BFA | 0.014 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E21 | TUN | 87.5 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E22 | TUN | 14.4 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | E23 | TUN | 1 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | F02 | NIT | 599.29 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | F03 | NIT | 479.43 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | F04 | NIT | 359.57 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | F05 | NIT | 239.72 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | F06 | NIT | 119.86 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | F07 | NIT | 59.93 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 | |
| 20180824_SBP-TQ_A20_rep1 | F08 | NIT | 29.96 | uM | 20180824_SBP-TQ_A20_rep1 | 8-72 (h) | 1 | 20180824_SBP-TQ_A20_rep1_GFP.nd2 |
