Selected Cell
Cell:
Value:
InvestigationInformation
StudyInformation
AssayInformation
AssayConditions
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| #Template info | |||||||||
| #TemplateVersion | LEI-MIHCSME_userTemplate_v3 | version 3 is updated version including recommendations by users, plus ISA and REMBI compliant. | |||||||
| #DateTemplateGenerated | Jan-20-2023 | ||||||||
| #CreatedBy | RH | ||||||||
| Annotation_groups | Key | Value | Comments_User | Description_Examples_Values | |||||
| DataOwner | First Name | Esmee | First Name | ||||||
| DataOwner | Middle Name(s) | Middle Name(s) if any | |||||||
| DataOwner | Last Name | Koedoot | Last Name | ||||||
| DataOwner | User name | koedoote | Institutional user name | ||||||
| DataOwner | Institute | Leiden University, Faculty of Science, Leiden Academic Centre for Drug Research | Institute level, eg. Universiteit Leiden, Faculty of Science, Institute of Biology | ||||||
| DataOwner | E-Mail Address | e.koedoot@lacdr.leidenuniv.nl | Institution email address | ||||||
| DataOwner | ORCID investigator | https://orcid.org/0000-0002-9041-0075 | orcid id as url, eg. https://orcid.org/0000-0002-3704-3675 | ||||||
| DataCollaborator | ORCID Data Collaborator | ORCID is of collaborator with experimental, collection or analysis part of this investigation | |||||||
| DataCollaborator | ORCID Data Collaborator | ORCID is of collaborator with experimental, collection or analysis part of this investigation | |||||||
| InvestigationInformation | Project ID | NA | EU/NWO/consortium ID – Examples: EuTOX | ||||||
| InvestigationInformation | Investigation Title | RNA splicing in breast cancer progression | High level concept to link related studies. Could be the main hypothesis/question you want to investigate as part of your research. Could be a chapter/thesis of your work. | ||||||
| InvestigationInformation | Investigation internal ID | NA | Corresponding internal ID for your investigation. | ||||||
| InvestigationInformation | Investigation description | investigating the signaling landscape controlling breast cancer cell migration | Short description for your investigation. | ||||||
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| # comments: | |||||||||
| # | |||||||||
| # | |||||||||
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| Annotation_groups | Key | Value | Comments_User | Description_Examples_Values | |||||
| Study | Study Title | Identification of metastasis driver genes controlling breast cancer cell migration using siRNA screening | Manuscript/chapter/publication/paragraph title describing purpose or intention for one or multiple assays (screens/experiments) | ||||||
| Study | Study internal ID | NA | Study ID, linked to ELN or lab journal | ||||||
| Study | Study Description | Triple-negative breast cancer (TNBC) is an aggressive and highly metastatic breast cancer subtype. Enhanced TNBC cell motility is a prerequisite of TNBC cell dissemination. Here | Description of study with additional unstructured information about this study possibly not covered elsewhere in this template. | ||||||
| Study | Study Key Words | [RNAi screening, systems microscopy] | List of key words associated with your study (EFO-terms). Examples: FDA approved drugs, Kinase inhibitors, DNA damage response, Unfolded protein response, Oxidative stress response, Inflammatory signaling, chemical similarity | ||||||
| Biosample | Biosample Taxon | NCBITAXON:9606 | NCBI-taxon id, human = NCBITAXON:9606; | ||||||
| Biosample | Biosample description | TNBC cell lines (Hs578T and MDA-MB-231) | Description of biosample genotype; Additional unstructured information about organisms used in this experiment. | ||||||
| Biosample | Biosample Organism | Homo sapiens | Which organism is your cell lines or tissue from. Examples: Human or mouse | ||||||
| Biosample | Number of cell lines used | 2 | In case multiple cell lines are used indicate here | ||||||
| Library | Library File Name | idr0022-screenA-annotation | Library file info | ||||||
| Library | Library File Format | tab-delimited text | Library file info | ||||||
| Library | Library Type | siRNA library | Library file info | ||||||
| Library | Library Manufacturer | Dharmacon | Library file info | ||||||
| Library | Library Version | NA | Library file info | ||||||
| Library | Library Experimental Conditions | NA | Any experimental conditions some cells were grown under as part of the study enter the information here e.g. different environmental stress conditions, or mutant background compared to wild type. | ||||||
| Library | Quality Control Description | NA | A brief description of the kind of quality control measures that were taken. | ||||||
| Protocols | HCS library protocol | NA | Url/doi protocols.io or ELN associated url. At least SOP/protocol filename | ||||||
| Protocols | growth protocol | Transfection | Reverse transfections were performed at 50nM concentration using 0.3 µl INTERFERin (Polyplus) per well. Transfection reagent and siRNA were incubated for 20 minutes | Url/doi protocols.io or ELN associated url. At least SOP/protocol filename | |||||
| Protocols | treatment protocol | Phagokinetic track assay | Url/doi protocols.io or ELN associated url. At least SOP/protocol filename | ||||||
| Protocols | HCS data analysis protocol | Data analysis | Url/doi protocols.io or ELN associated url. At least SOP/protocol filename | ||||||
| Plate | Plate type | SensoPlate | Example: uclear | ||||||
| Plate | Plate type Manufacturer | Greiner Bio-One | Example: Greiner Bio-One | ||||||
| Plate | Plate type Catalog number | NA | Example:781081 | ||||||
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| Annotation_groups | Key | Value | Comments_User | Description_Examples_Values | |||||
| Assay | Assay Title | ScreenB | Screen name | ||||||
| Assay | Assay internal ID | NA | Experimental ID, e.g. aMV-010, linked to ELN or labjournal | ||||||
| Assay | Assay Description | Validation RNAi screen using smartpool and single siRNAs to validate cell migratory regulators in Hs578T and MDA-MB-231 cells that were identified in the primary screen. | validation screen | Description of screen plus additional unstructured information about screen not covered elsewhere. Stages of HCS: either primary screen, secondary screen or validation screen. EFO terms | |||||
| Assay | Assay number of biological replicates | 4 | single and smartpooled | Total number of biol. Repl. | |||||
| Assay | Number of plates | 152 | Total number of plates, n-plates | ||||||
| Assay | Assay Technology Type | bright-field microscopy | Imaging method, Fbbi terms. | ||||||
| Assay | Assay Type | high content screen of cells treated with library of siRNAs | List of options: sub types of HCS e.g. Gene Knock down, RNAi, compount, EFO terms | ||||||
| Assay | Assay External URL | https://idr.openmicroscopy.org/webclient/?show=screen-2152 | ELN or any other external url link to this screen. | ||||||
| Assay | Assay data URL | https://idr.openmicroscopy.org/webclient/?show=screen-2152 | OMERO url link to this screen. | ||||||
| AssayComponent | Imaging protocol | NA | Migratory tracks were visualized by acquiring whole-well montages (6 x 6 images) on a BD Pathway 855 BioImager using transmitted light and a x10 objective. | Url to protocols.io or protocols in ELN, at least protocol file name or free text description of imaging protocol | |||||
| AssayComponent | Sample preparation protocol | NA | Sample preparation method (Formaldahude (PFA) fixed tissue, Live cells, unfixed tissue). FBbi terms | ||||||
| Biosample | Cell lines storage location | NA | Storage location according to Database used or at least location e.g. Example -150 liquid N2 tank 4th floor GE4.xx | ||||||
| Biosample | Cell lines clone number | NA | Storage location DB info | ||||||
| Biosample | Cell lines Passage number | NA | Passage number of your cells. In case different passage number is used for the replicates, please indicate it in Comment_users. | ||||||
| ImageData | Image number of pixelsX | 672 | Indicate number of pixels in x in images | ||||||
| ImageData | Image number of pixelsY | 512 | Indicate number of pixels in y in images | ||||||
| ImageData | Image number of z-stacks | 1 | Indicate number z stacks in image, single image is z=1 | ||||||
| ImageData | Image number of channels | 1 | Indicate number of channels in your image, single channel image c=1, including your TD channel | ||||||
| ImageData | Image number of timepoints | 1 | Indicate number of time point(s) in your image | ||||||
| ImageData | Image sites per well | 36 | Number of fields, numeric value | ||||||
| ImageAcquisition | Microscope id | BD-pathway | Url to micrometa app file link or other url describing your microscope. At least Microscope name. | ||||||
| Specimen | Channel Transmission id | 1 | only transmission channel is recorded for this study. | Channel id is dependent on different machines, first or last. If No transmission is acquired state NA | |||||
| Specimen | Channel 1 visualization method | Visualization method (example: Hoechst staining ) | |||||||
| Specimen | Channel 1 entity | Entity visualized (example: DNA ) | |||||||
| Specimen | Channel 1 label | Label used for entity (example:Nuclei) Best practice to use similar label as your CP-pipeline | |||||||
| Specimen | Channel 1 id | sequential id of channel order in your image | |||||||
| Specimen | Channel 2 visualization method | Visualization method (example: GFP ) | |||||||
| Specimen | Channel 2 entity | Entity visualized (example: MAP1LC3B ) | |||||||
| Specimen | Channel 2 label | Label used for entity (example:GFP-LC3) Best practice to use similar label as your CP-pipeline | |||||||
| Specimen | Channel 2 id | sequential id of channel order in your image | |||||||
| Specimen | Channel 3 visualization method | Visualization method (example: PI staining ) | |||||||
| Specimen | Channel 3 entity | Entity visualized (example: Death cell DNA ) | |||||||
| Specimen | Channel 3 label | Label used for entity (example:PI) Best practice to use similar label as your CP-pipeline | |||||||
| Specimen | Channel 3 id | sequential id of channel order in your image | |||||||
| Specimen | Channel 4 visualization method | Visualization method (example: Apoptotic marker Annexin V staining ) | |||||||
| Specimen | Channel 4 entity | Entity visualized (example: Apoptotic cells ) | |||||||
| Specimen | Channel 4 label | Label used for entity (example:Annexin-V) Best practice to use similar label as your CP-pipeline | |||||||
| Specimen | Channel 4 id | sequential id of channel order in your image |
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| MDA231_plate_91d | C12 | siRNA | 50 | nmol | MDAMB231 | 72 | 30 | MDA231_plate_91d | 91d | C12 | 36 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | DNM2 | 1785 | NM_004945 | 56549118 | Positive | M-004007-01 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||||
| MDA231_plate_91d | C2 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | C2 | 26 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | MTMR3 | 8897 | NM_153051 | 148276975 | D-008039-05 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | C3 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | C3 | 27 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | PRPF19 | 27339 | NM_014502 | 34222313 | D-004668-01 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | C4 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | C4 | 28 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | ACVR1 | 90 | NM_001105 | 10862690 | D-004924-02 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | C5 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | C5 | 29 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | FLJ23356 | 84197 | NM_032237 | 26024341 | D-005321-02 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | C6 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | C6 | 30 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | MTMR8 | 55613 | NM_017677 | 21361758 | D-008042-01 | D-008042-02 | D-008042-03 | D-008042-05 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||
| MDA231_plate_91d | C7 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | C7 | 31 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | FBXO30 | 84085 | NM_032145 | 54112383 | D-021407-02 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | C8 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | C8 | 32 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | IGF1R | 3480 | NM_000875 | 119220593 | D-003012-17 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | C9 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | C9 | 33 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | PPP1R3D | 5509 | NM_006242 | 37622904 | D-021439-03 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | D1 | siRNA | 50 | nmol | MDAMB231 | 72 | 30 | MDA231_plate_91d | 91d | D1 | 37 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | ILK | 3611 | NM_001014794 | 62420872 | M-004499-00 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | D10 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | D10 | 46 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | FLJ23356 | 84197 | NM_032237 | 26024341 | D-005321-01 | D-005321-02 | D-005321-03 | D-005321-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||
| MDA231_plate_91d | D11 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | D11 | 47 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | USP18 | 11274 | NM_017414 | 156071510 | D-004236-04 | D-004236-19 | D-004236-20 | D-004236-21 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||
| MDA231_plate_91d | D12 | siRNA | 50 | nmol | MDAMB231 | 72 | 46 | MDA231_plate_91d | 91d | D12 | 48 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | mock | Negative | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||||||
| MDA231_plate_91d | D2 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | D2 | 38 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | IGF1R | 3480 | NM_000875 | 119220593 | D-003012-08 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | D3 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | D3 | 39 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | HIPK1 | 204851 | NM_152696 | 38201637 | D-004809-22 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | D4 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | D4 | 40 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | USP18 | 11274 | NM_017414 | 156071510 | D-004236-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | D5 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | D5 | 41 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | MYLK | 4638 | NM_053031 | 47132571 | D-005351-05 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | D6 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | D6 | 42 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | PDK2 | 5164 | NM_002611 | 31543388 | D-005020-03 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | D7 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | D7 | 43 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | NME2 | 4831 | NM_001018139 | 66392226 | D-005102-05 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | D8 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | D8 | 44 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | SENP1 | 29843 | NM_014554 | 7657549 | D-006357-02 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | D9 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | D9 | 45 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | HIPK1 | 204851 | NM_152696 | 38201637 | D-004809-21 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | E1 | siRNA | 50 | nmol | MDAMB231 | 72 | 47 | MDA231_plate_91d | 91d | E1 | 49 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | mock | Negative | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||||||
| MDA231_plate_91d | E10 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | E10 | 58 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | PPP1R3D | 5509 | NM_006242 | 37622904 | D-021439-01 | D-021439-03 | D-021439-04 | D-021439-05 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||
| MDA231_plate_91d | E11 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | E11 | 59 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | UBE3A | 7337 | NM_000462 | 19718765 | D-005137-01 | D-005137-02 | D-005137-03 | D-005137-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||
| MDA231_plate_91d | E12 | siRNA | 50 | nmol | MDAMB231 | 72 | 31 | MDA231_plate_91d | 91d | E12 | 60 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | ILK | 3611 | NM_001014794 | 62420872 | M-004499-00 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | E2 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | E2 | 50 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | PPP1R3D | 5509 | NM_006242 | 37622904 | D-021439-01 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | E3 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | E3 | 51 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | PPP1R3D | 5509 | NM_006242 | 37622904 | D-021439-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | E4 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | E4 | 52 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | UBE3A | 7337 | NM_000462 | 19718765 | D-005137-03 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | E5 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | E5 | 53 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | NME2 | 4831 | NM_001018139 | 66392226 | D-005102-03 | D-005102-04 | D-005102-05 | D-005102-06 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||
| MDA231_plate_91d | E6 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | E6 | 54 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | MTMR8 | 55613 | NM_017677 | 21361758 | D-008042-02 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | E7 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | E7 | 55 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | CUL3 | 8452 | NM_003590 | 45827792 | D-010224-05 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | E8 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | E8 | 56 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | MYLK | 4638 | NM_053031 | 47132571 | D-005351-17 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | E9 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | E9 | 57 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | PRPF19 | 27339 | NM_014502 | 34222313 | D-004668-01 | D-004668-02 | D-004668-04 | D-004668-17 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||
| MDA231_plate_91d | F1 | siRNA | 50 | nmol | MDAMB231 | 72 | 31 | MDA231_plate_91d | 91d | F1 | 61 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | DNM2 | 1785 | NM_004945 | 56549118 | Positive | M-004007-01 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||||
| MDA231_plate_91d | F10 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | F10 | 70 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | UBE3A | 7337 | NM_000462 | 19718765 | D-005137-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | F11 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | F11 | 71 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | MTMR8 | 55613 | NM_017677 | 21361758 | D-008042-01 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | F12 | siRNA | 50 | nmol | MDAMB231 | 72 | 32 | MDA231_plate_91d | 91d | F12 | 72 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | DNM2 | 1785 | NM_004945 | 56549118 | Positive | M-004007-01 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||||
| MDA231_plate_91d | F2 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | F2 | 62 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | SENP1 | 29843 | NM_014554 | 7657549 | D-006357-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | F3 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | F3 | 63 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | PRPF19 | 27339 | NM_014502 | 34222313 | D-004668-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | F4 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | F4 | 64 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | FBXO30 | 84085 | NM_032145 | 54112383 | D-021407-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | F5 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | F5 | 65 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | MTMR8 | 55613 | NM_017677 | 21361758 | D-008042-05 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | F6 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | F6 | 66 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | HIPK1 | 204851 | NM_152696 | 38201637 | D-004809-06 | D-004809-21 | D-004809-22 | D-004809-23 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||
| MDA231_plate_91d | F7 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | F7 | 67 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | ACVR1 | 90 | NM_001105 | 10862690 | D-004924-03 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | F8 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | F8 | 68 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | USP18 | 11274 | NM_017414 | 156071510 | D-004236-21 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | F9 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | F9 | 69 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | FBXO30 | 84085 | NM_032145 | 54112383 | D-021407-03 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | G1 | siRNA | 50 | nmol | MDAMB231 | 72 | 48 | MDA231_plate_91d | 91d | G1 | 73 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | mock | Negative | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||||||
| MDA231_plate_91d | G10 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | G10 | 82 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | FLJ23356 | 84197 | NM_032237 | 26024341 | D-005321-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | G11 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | G11 | 83 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | SENP1 | 29843 | NM_014554 | 7657549 | D-006357-03 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | G12 | siRNA | 50 | nmol | MDAMB231 | 72 | 49 | MDA231_plate_91d | 91d | G12 | 84 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | mock | Negative | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||||||
| MDA231_plate_91d | G2 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | G2 | 74 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | MYLK | 4638 | NM_053031 | 47132571 | D-005351-07 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | G3 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | G3 | 75 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | ACVR1 | 90 | NM_001105 | 10862690 | D-004924-18 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | G4 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | G4 | 76 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | USP18 | 11274 | NM_017414 | 156071510 | D-004236-20 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | G5 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | G5 | 77 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | FLJ23356 | 84197 | NM_032237 | 26024341 | D-005321-03 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | G6 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | G6 | 78 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | PDK2 | 5164 | NM_002611 | 31543388 | D-005020-01 | D-005020-02 | D-005020-03 | D-005020-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||
| MDA231_plate_91d | G7 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | G7 | 79 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | UBE3A | 7337 | NM_000462 | 19718765 | D-005137-01 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | G8 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | G8 | 80 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | IGF1R | 3480 | NM_000875 | 119220593 | D-003012-18 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | G9 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | G9 | 81 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | PRPF19 | 27339 | NM_014502 | 34222313 | D-004668-02 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | H1 | siRNA | 50 | nmol | MDAMB231 | 72 | 32 | MDA231_plate_91d | 91d | H1 | 85 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | ILK | 3611 | NM_001014794 | 62420872 | M-004499-00 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | H10 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | H10 | 94 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | MYLK | 4638 | NM_053031 | 47132571 | D-005351-05 | D-005351-06 | D-005351-07 | D-005351-17 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||
| MDA231_plate_91d | H11 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | H11 | 95 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | ACVR1 | 90 | NM_001105 | 10862690 | D-004924-01 | D-004924-02 | D-004924-03 | D-004924-18 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||
| MDA231_plate_91d | H12 | siRNA | 50 | nmol | MDAMB231 | 72 | 16 | MDA231_plate_91d | 91d | H12 | 96 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | GFP | P-002048-01 | D-001300-01 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||||||||
| MDA231_plate_91d | H2 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | H2 | 86 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | USP18 | 11274 | NM_017414 | 156071510 | D-004236-19 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | H3 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | H3 | 87 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | HIPK1 | 204851 | NM_152696 | 38201637 | D-004809-06 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | H4 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | H4 | 88 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | CUL3 | 8452 | NM_003590 | 45827792 | D-010224-03 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | H5 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | H5 | 89 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | IGF1R | 3480 | NM_000875 | 119220593 | D-003012-06 | D-003012-08 | D-003012-17 | D-003012-18 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||
| MDA231_plate_91d | H6 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | H6 | 90 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | SENP1 | 29843 | NM_014554 | 7657549 | D-006357-01 | D-006357-02 | D-006357-03 | D-006357-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | |||||
| MDA231_plate_91d | H7 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | H7 | 91 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | PDK2 | 5164 | NM_002611 | 31543388 | D-005020-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | H8 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | H8 | 92 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | NME2 | 4831 | NM_001018139 | 66392226 | D-005102-06 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_91d | H9 | siRNA | 50 | nmol | MDAMB231 | 72 | 4 | MDA231_plate_91d | 91d | H9 | 93 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | MTMR8 | 55613 | NM_017677 | 21361758 | D-008042-03 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_91d | ||||||||
| MDA231_plate_92a | A1 | siRNA | 50 | nmol | MDAMB231 | 72 | 17 | MDA231_plate_92a | 92a | A1 | 1 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | GFP | P-002048-01 | D-001300-01 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | |||||||||||
| MDA231_plate_92a | A10 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | A10 | 10 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | LTB4R2 | 56413 | NM_019839 | 141803218 | D-005654-05 | D-005654-18 | D-005654-19 | D-005654-20 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | |||||
| MDA231_plate_92a | A11 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | A11 | 11 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | RNF32 | 140545 | NM_030936 | 37595535 | D-007136-01 | D-007136-02 | D-007136-04 | D-007136-05 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | |||||
| MDA231_plate_92a | A12 | siRNA | 50 | nmol | MDAMB231 | 72 | 33 | MDA231_plate_92a | 92a | A12 | 12 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | ILK | 3611 | NM_001014794 | 62420872 | M-004499-00 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | A2 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | A2 | 2 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | RYR2 | 6262 | NM_001035 | 112799846 | D-006293-01 | D-006293-02 | D-006293-04 | D-006293-17 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | |||||
| MDA231_plate_92a | A3 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | A3 | 3 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | TRIM39 | 56658 | NM_172016 | 25777697 | D-007028-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | A4 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | A4 | 4 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | PIP5K2A | 5305 | NM_005028 | 156416001 | D-006778-01 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | A5 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | A5 | 5 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | DTX3 | 196403 | NM_178502 | 31341899 | D-007156-02 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | A6 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | A6 | 6 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | VIPR1 | 7433 | NM_004624 | 15619005 | D-005749-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | A7 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | A7 | 7 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | PRKWNK1 | 65125 | NM_018979 | 148612808 | D-005362-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | A8 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | A8 | 8 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | DZIP3 | 9666 | NM_014648 | 40254857 | D-006910-03 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | A9 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | A9 | 9 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | SSTR1 | 6751 | NM_001049 | 33946330 | D-003711-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | B1 | siRNA | 50 | nmol | MDAMB231 | 72 | 50 | MDA231_plate_92a | 92a | B1 | 13 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | mock | Negative | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||||||
| MDA231_plate_92a | B10 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | B10 | 22 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | RING1 | 6015 | NM_002931 | 51479191 | D-006554-03 | D-006554-04 | D-006554-05 | D-006554-06 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | |||||
| MDA231_plate_92a | B11 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | B11 | 23 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | VIPR1 | 7433 | NM_004624 | 15619005 | D-005749-01 | D-005749-02 | D-005749-03 | D-005749-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | |||||
| MDA231_plate_92a | B12 | siRNA | 50 | nmol | MDAMB231 | 72 | 51 | MDA231_plate_92a | 92a | B12 | 24 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | mock | Negative | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||||||
| MDA231_plate_92a | B2 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | B2 | 14 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | DZIP3 | 9666 | NM_014648 | 40254857 | D-006910-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | B3 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | B3 | 15 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | RING1 | 6015 | NM_002931 | 51479191 | D-006554-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | B4 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | B4 | 16 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | E2F4 | 1874 | NM_001950 | 44829053 | D-003262-23 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | B5 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | B5 | 17 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | BPTF | 2186 | NM_004459 | 119395732 | D-004025-03 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | B6 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | B6 | 18 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | LTB4R2 | 56413 | NM_019839 | 141803218 | D-005654-05 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | B7 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | B7 | 19 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | CEBPD | 1052 | NM_005195 | 125661056 | D-010453-01 | D-010453-02 | D-010453-03 | D-010453-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | |||||
| MDA231_plate_92a | B8 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | B8 | 20 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | E2F4 | 1874 | NM_001950 | 44829053 | D-003262-05 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | B9 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | B9 | 21 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | BGLAP | 632 | NM_000711 | 158517828 | D-008633-01 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | C1 | siRNA | 50 | nmol | MDAMB231 | 72 | 33 | MDA231_plate_92a | 92a | C1 | 25 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | DNM2 | 1785 | NM_004945 | 56549118 | Positive | M-004007-01 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | |||||||
| MDA231_plate_92a | C10 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | C10 | 34 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | RYR2 | 6262 | NM_001035 | 112799846 | D-006293-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | C11 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | C11 | 35 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | PRKWNK1 | 65125 | NM_018979 | 148612808 | D-005362-01 | D-005362-02 | D-005362-03 | D-005362-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | |||||
| MDA231_plate_92a | C12 | siRNA | 50 | nmol | MDAMB231 | 72 | 34 | MDA231_plate_92a | 92a | C12 | 36 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | DNM2 | 1785 | NM_004945 | 56549118 | Positive | M-004007-01 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | |||||||
| MDA231_plate_92a | C2 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | C2 | 26 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | DTX3 | 196403 | NM_178502 | 31341899 | D-007156-03 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | ||||||||
| MDA231_plate_92a | C3 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | C3 | 27 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | smartpool | BGLAP | 632 | NM_000711 | 158517828 | D-008633-01 | D-008633-02 | D-008633-03 | D-008633-04 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a | |||||
| MDA231_plate_92a | C4 | siRNA | 50 | nmol | MDAMB231 | 72 | 1 | MDA231_plate_92a | 92a | C4 | 28 | Homo sapiens | NCBITaxon | NCBITaxon_9606 | MDAMB231 | EFO | EFO_0001209 | single | BPTF | 2186 | NM_004459 | 119395732 | D-004025-01 | ./Validation PKT screen MDA-MB-231/MDA231 hits validation set2/MDA231_plate_92a |
