Characterization of flux distribution and general physiology of L. lactis in continuous cultures

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Value:

Metadata Environmental parameters Measurements environ parameters Medium composition Medium composition (measured) Extracellular metabolites Calculated fluxes Relative flux distributions

100

Item	Value
Experimentator	Martijn
Raw data
Reference	SYSMO-LAB

Description	Growth in glucose-limited chemostat conditions at low growth rates with slow increases in oxygenation
Aim	Effect of aeration of pyruvate branching
Strain information	NZ9000
Strain	Lactococcus lactis
Vessel type	Applikon fermentor
Stirring rate	400	RPM
Temperature	37	degree celcius
Dilution rate	factor studied	/h
Volume	1000	ml
Gas flow	500	ml
pH	6.5
SOP's used
Jermtype	1

Explanations	Data

TOPIC
Titel	Carbon metabolism and its response to different dilutions rate in glucose-limited chemsotat conditions
Comments	some comments

ASSAY
Title	In vivo metabolites concentrations
Assay_type	Catabolic flux distributions
Comments

EXPERIMENT
Experiment_description	chemostat 1
Experiment_type	time series
Experimentalist	Martijn Bekker

Begin_date	39619
User	your name
Group	your group
SOP	SOP 1,4

ORGANISM
Organism	L. lactis
Strain	NZ9000
Genotype	wild-type
Phenotype	wild-type
Culture_starting_date	varying
Date_at_sampling	varying
Age_at_sampling_hours	varying


Experimental_conditions
Item	pH	temperature	buffer
Start_value	6.5	37	1
End_value	6.5	37
Unit	dimensionlesss	ºC
Comments


FACTORS_STUDIED
Item	Dilution rate
Start_value	0.15
End_value
Unit	per hour
Comments	Growth rate dependent flux distribution


PUBLICATION
Title	1
Authors	1
Journal	1
URL	1
Year	1
Volume	1
Pages	1
PubmedID	1
UserID	1
GroupID	1
ProjectID	1
ExperimentID	1

	experiment run	measurementnumber	Age	Date	Chemostat	Medium flow	Volume	Temperature	pH Chemostat	Dilution rate	%O2 input	Airflow	N2 flow	Total flow	Pump	Comments
Unit	1	1	hours	Date	name	ml/hour	ml	Celcius	1	1/hour	volume%	ml/min	ml/min	ml/min	1	1
	1	1		14/02/08	SYSMO3	50	1000	37	6.5	0.05	0	0	500	500		Old chemostat, however looks good
	2	1		25/02/08	SYSMO4	50	1000	37	6.5	0.05	0	0	500	500		Looks good, little filaments
	3	1		28/2/2008	SYSMO4	75	500	37	6.5	0.15	0	0	500	500		Looks good, little filaments
	4	1		2007-03-10T0:0:0+0000	SYSMO3	75	500	37	7.5	0.15	0	0	500	500		Looks good, little filaments
	5	1		21/11/2007	SYSMO3	50	1000	37	7.5	0.05	0	0	500	500		Looks good, little filaments
	6	1		25/2/2008	SYSMO3	75	500	37	6.5	0.15	0	0	500	500		Looks good, no filaments att all
	7	1		21/3/2008	SYSMO3	75	500	37	7.5	0.15	0	0	500	500		Looks good, little filaments
	8	1		28/9/2007	SYSMO4	75	500	37	7.5	0.15	0	0	500	500		Looks good, no filaments att all
	8	2		2007-01-10T0:0:0+0000	SYSMO4	75	500	37	6.5	0.15	0	0	500	500		Looks good, no filaments att all
	9	1		2007-09-04T0:0:0+0000	SYSMO4	50	1000	37	6	0.05	0	0	500	500		Looks good, no filaments att all
	10	1	72	24/8/2009	SA	15	100	37	6.5	0.15	0	0	100	100	26	High arginine, looks good, no filaments at all
	10	2	96	25/8/2009	SA	25	100	37	6.5	0.25	0	0	100	100	92	High arginine, looks good, many filaments of around 4-8 cells
	10	3	120	25/8/2009	SA	40	100	37	6.5	0.4	0	0	100	100	168	High arginine, looks good, many filaments of around 2-4 cells
	11	1	144	2009-09-03T0:0:0+0000	SYSMO3	50	1000	37	6.5	0.05	0	0	100	100	0.8	High arginine, looks good, many filaments of around 10-20 cells
	11	2	168	2009-09-03T0:0:0+0000	SYSMO3	50	1000	37	6.5	0.05	0	0	100	100	0.8	High arginine, looks good, many filaments of around 10-20 cells
	12	1	72	31/8/2009	SA	30	100	37	6.5	0.3	0	0	100	100	112	High arginine, looks good, no filaments at all
	12	2	96	2009-09-01T0:0:0+0000	SA	35	100	37	6.5	0.35	0	0	100	100	133	High arginine, looks good, many filaments of around 4-8 cells
	13	1	96	2009-09-01T0:0:0+0000	SA	15	100	37	6.5	0.15	0	0	100	100	26	Very high arginine, looks good, almost no filaments
	13	2	120	2009-09-01T0:0:0+0000	SA	35	100	37	6.5	0.35	0	0	100	100	133	Culture showed very low density. Stopped pump and set te D=0.15
	13	3	144	2009-09-01T0:0:0+0000	SA	15	100	37	6.5	0.15	0	0	100	100	26
	14	1	144	20/01/10	Sysmo4	50	1000	37	7.5	0.05	0	0	100	100	72	No glucose, culture look OK, many filaments
	15	1	72	2010-01-02T0:0:0+0000	SA1	12	100	37	7.5	0.12	0	0	100	100	17	Culture looked OK
	15	1	72	2010-01-02T0:0:0+0000	SA2	12	100	37	7.5	0.12	0	0	100	100	17	Culture looked OK
	15	1	72	2010-01-02T0:0:0+0000	SA3	12	100	37	7.5	0.12	0	0	100	100	17	Culture looked OK
	16	1	120	2010-01-02T0:0:0+0000	SA2	12	100	37	6.5	0.12	0	0	100	100	17	Culture looked OK
	16	1	120	2010-01-02T0:0:0+0000	SA3	12	100	37	6.5	0.12	0	0	100	100	17	Culture looked OK
	17	1	96	19/2/2010	SYSMO1	150	1000	37	7.5	0.15	0	0	100	100	1.5	OD and dryweight too low, found residual glucose in HPLC sample
	17	2	96	23/2/2010	SYSMO1	50	1000	37	7.5	0.05	0	0	100	100	0.7	Dryweight estimated from OD
	18	1	96	2010-03-04T0:0:0+0000	SA1	35	100	37	6.5	0.35	0	0	100	100	100	Culture looked OK
	18	2	96	2010-03-04T0:0:0+0000	SA2	35	100	37	6.5	0.35	0	0	100	100	100	Culture looked OK
	19	1	120	22/03/2010	SA1	10	100	37	6.5	0.1	0	0	100	100	16	Culture looked OK
	19	2	120	22/03/2010	SA3	10	100	37	6.5	0.1	0	0	100	100	16	Culture looked OK

Identifier	experiment run	measurementnumber	O2	O2	CO2	pH	RPM	OD	standard deviation OD	Dry weight	standard deviation dryweight
Unit	1	1	volume%	volume%	volume%	1	rpm	AU	AU	gram/liter	gram/liter
Description			Inputgas	Outputgas	Outputgas		stirring rate	Optical density (600nm)	Optical density (600nm)	Dryweight measured as described in SOP X
	1	1	0	0	0	6.4	400	3.6		0.97
	2	1	0	0	0		400	3.2		0.94
	3	1	0	0	0		400	3.45		1.05
	4	1	0	0	0		400			0.39
	5	1	0	0	0	7.51	400	1.6		0.45
	6	1	0	0	0	6.54	400	2		1.03
	7	1	0	0	0		400	1.8		0.48
	8	1	0	0	0	7.48	400			0.4
	8	2	0	0	0	6.46	400			0.88
	9	1	0	0	5.7E-4		400	2.5		0.84
	10	1	0	0	0	6.4	slow	3.32		1.33	0.04
	10	2	0	0	0	6.32	slow	4.42	0	1.115	0.09192388155425034
	10	3	0	0	0	6.2	slow	3.465	0.0636396103067849	0.905	0.021213203435597874
	11	1	0	0	0	6.2	400	3.465	0.0636396103067849	0.905	0.021213203435597874
	11	2	0	0	3.3E-4	6.46	400	2.825	0.04949747468303654	0.625	0.04949747468305896
	12	1	0	0	0	6.52	slow	3.985	0.2616295090390198	1.09	0
	12	2	0	0	0		slow			1.285	0.09192388155424792
	13	1	0	0	0	6.38	slow			0.805	0.007071067811872936
	13	2	0	0	0		slow
	13	3	0	0	0		slow
	14	1	0	0	0	7.4	400	1.41	0.028284271247460344	0.3649999999999931	0.03535533905943376
	15	1	0	0	0	7.71	slow	2.1	0.014142135623840079	0.39999999999995595	0.04242640687109435
	15	1	0	0	0	7.6	slow	2.235	0.09192388155425034	0.5150000000000432	0.007071067811849385
	15	1	0	0	0	7.63	slow	1.835	0.09192388155425517	0.4100000000001103	0.11313708498983524
	16	1	0	0	0	6.62	slow	3.41	0.042426406871101545	1.0550000000000281	0.035355339059242215
	16	1	0	0	0	6.55	slow	4	0.282842712474616	1.0899999999999466	0.09899494936613587
	17	1	0	0	0	7.35	400	0.625	0.13435028842544408	0.14499999999992852	0.06363961030676653
	17	2	0	0	0	7.47	400	1.1	0.028284271247460344	0.33
	18	1	0	0	0		slow	4.315	0.06363961030684073	1.3
	18	2	0	0	0		slow	3	0.12727922061358377	1.1
	19	1	0	0	0	6.67	slow	2.065	0.20506096654409806	0.9049999999999336	0.007071067811967142
	19	2	0	0	0	6.57	slow	2.885	0.26162950903902665	1.0550000000000281	0.09192388155428899
	0	0	0	0	0		slow
	0	0	0	0	0		slow
	0	0	0	0	0		slow
	0	0	0	0	0		slow
	0	0	0	0	0		slow
	0	0	0	0	0		slow
	0	0	0	0	0		slow
	0	0	0	0	0		slow
	0	0	0	0	0		slow











						After	Before	ml used	dryweight	OD	average	stdev	average	stdev
						11.1521	11.1409	10	1.1200000000000543	2.7	2.885	0.26162950903902665	1.0550000000000281	0.09192388155428899
						11.3788	11.3689	10	0.990000000000002	3.07

	Unit
Compound	1	Glucose	Ethanol	Acetate	Lactate	Formiate	Succinate	Pyruvate	Acetoin	2,3 Butanediol	Aspartic acid	Serine	Glutamate	Glycine	Histidine	NH3	Arginine	Threonine	Alanine	Proline	Cysteine	Tyrosine	Valine	Methionine	Ornithine	Lysine	Isoleucine	Leucine	Phenylalanine
Concentration	mM	61.1	0	12.2	0	0	0	0	0	0	3.16	3.24	3.4	2.33	0.97		2.37	1.89	3.26	5.86	1.07	1.38	2.77	0.84	0	2.41	1.6	3.62	1.66

Identifier	Experiment	measurementnumber	Glucose	Ethanol	Acetate	Lactate	Formiate	Succinate	Pyruvate	Acetoin	2,3 Butanediol	%O2	CO2	Aspartic acid	Serine	Glutamate	Glycine	Histidine	NH3	Arginine	Threonine	Alanine	Proline	Cysteine	Tyrosine	Valine	Methionine	Ornithine	Lysine	Isoluecine	Leucine	Phenylalanine
Unit			mM	mM	mM	mM	mM	mM	mM	mM	mM	%	%	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	μM	mM	mM	mM	mM
	1	1	61.1	0	12.2	0	0	0	0	0	0	0	0
	2	1	61.1	0	12.2	0	0	0	0	0	0	0	0
	3	1	61.1	0	12.2	0	0	0	0	0	0	0	0
	4	1	61.1	0	12.2	0	0	0	0	0	0	0	0
	5	1	61.1	0	12.2	0	0	0	0	0	0	0	0
	6	1	61.1	0	12.2	0	0	0	0	0	0	0	0
	7	1	61.1	0	12.2	0	0	0	0	0	0	0	0
	8	1	61.1	0	12.2	0	0	0	0	0	0	0	0
	8	2	61.1	0	12.2	0	0	0	0	0	0	0	0
	9	1	61.1	0	12.2	0	0	0	0	0	0	0	5.7E-4
	10	1	57.0622	0	12.1428	0	0	0	0	0	0	0	0	0.2524	0.3518	0.3002	0.2377	0.1195	0.5889	0.2372	0.1629	0.2207	0.5029		0.1275	0.2719	0.0658		0.1892	0.136	0.2945	0.1531
	10	2	57.0622	0	12.1428	0	0	0	0	0	0	0	0	0.2524	0.3518	0.3002	0.2377	0.1195	0.5889	0.2372	0.1629	0.2207	0.5029		0.1275	0.2719	0.0658		0.1892	0.136	0.2945	0.1531
	10	3	57.0622	0	12.1428	0	0	0	0	0	0	0	0	0.2524	0.3518	0.3002	0.2377	0.1195	0.5889	0.2372	0.1629	0.2207	0.5029		0.1275	0.2719	0.0658		0.1892	0.136	0.2945	0.1531
	11	1	60.3	0	12.8	0	0	0	0	0	6.2	0	0
	11	2	60.3	0	12.8	0	0	0	0	0	6.2	0	3.3E-4
	12	1	57.0622	0	12.1428	0	0	0	0	0	0	0	0
	12	2	57.0622	0	12.1428	0	0	0	0	0	0	0	0
	13	1	61.1	0	12.2	0	0	0	0	0		0	0
	13	2	61.1	0	12.2	0	0	0	0	0	0	0	0
	13	3	61.1	0	12.2	0	0	0	0	0	0	0	0
	14	1	61.1	0	12.2	0	0	0	0	0	0	0	0
	15	1	61.1	0	12.2	0	0	0	0	0
	15	1	61.1	0	12.2	0	0	0	0	0
	15	1	61.1	0	12.2	0	0	0	0	0
	16	1	61.1	0	12.2	0	0	0	0	0
	16	1	61.1	0	12.2	0	0	0	0	0
	17	1	61.1	0	12.2	0	0	0	0	0
	17	2	61.1	0	12.2	0	0	0	0	0
	18	1	75.6068	0	12.5312	0	0	0	0	0.4669
	18	2	75.6068	0	12.5312	0	0	0	0	0.4669
	19	1	61.1	0	12.2	0	0	0	0	0
	19	2	61.1	0	12.2	0	0	0	0	0
	0	0	61.1	0	12.2	0	0	0	0	0
	0	0	61.1	0	12.2	0	0	0	0	0
	0	0	61.1	0	12.2	0	0	0	0	0
	0	0	61.1	0	12.2	0	0	0	0	0
	0	0	61.1	0	12.2	0	0	0	0	0
	0	0	61.1	0	12.2	0	0	0	0	0

Identifier	Experiment	measurementnumber	Glucose	Ethanol	Acetate	Lactate	Formiate	Succinate	Pyruvate	Acetoin	2,3 Butanediol	O2	CO2	Aspartic acid	Serine	Glutamate	Glycine	Histidine	NH3	Arginine	Threonine	Alanine	Proline	Cysteine	Tyrosine	Valine	Methionine	Ornithine	Lysine	Isoluecine	Leucine	Phenylalanine
Unit	1	1	mM	mM	mM	mM	mM	mM	mM	mM	mM	%	%	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	μM	mM	mM	mM	mM
	1	1	0	33.57	37.16	67.06	58.16	0.485	0	1.68	0.055
	2	1	0	22.26	31.54	81.52	47.18	0.24	0	0.95	1.55
	3	1	0	12.26	20.25	112.01	22.05	0.46	0.21	0.25	1.35
	4	1	0	4.86	15.54	85.65	8.915	0.275	0.905	0	1.335
	5	1	0	8.56	17.895	98.83000000000001	15.4825	0.36750000000000005	0.5575	0.125	1.3425
	6	1	0	11.51	21.94	81.25	25.29	0.14	0	0	1
	7	1	0	3.765	13.455	90.845	5.965	0.34	0.42	0	1.085
	8	1	0	10.145	19.235	64.43	18	0	0.74	0	0
	8	2	0	11.795	22.86	64.815	21.575	0	0.3	0	0
	9	1	0	11.23	19.72	81.14	21.95	0	0	0.88	1.5	0	5.7E-4
	10	1	0	16.4786	32.5007	25.8519	37.2169	0	0	0	0			0.3588	0.1088	0.31	0.1738	0.083	1.061	0.026	0.1235	0.2065	0.5823		0.0955	0.2096	0.0336	18.118	0.1832	0.0738	0.2329	0.1467
	10	2	0	10.2577	25.2728	50.8934	22.4953	0	0	0	0	0	0	0.2986	0.0598	0.253	0.1694	0.07	0.9389	0.0236	0.0979	0.163	0.5025		0.094	0.1918	0.0297	15.2448	0.1561	0.0793	0.2288	0.1295
	10	3	1.4612	0	13.5244	66.5927	3.983	0	0	0	0	0	0	0.3386	0.074	0.3276	0.2401	0.0855	1.1458	0.1048	0.1197	0.2037	0.6154		0.1204	0.2538	0.0381	13.1896	0.2075	0.1195	0.3056	0.1614
	11	1	0	12.3266	27.6089	46.7862	25.5899	0	0	0	0	0	0	0.5931	0.153	0.551	0.379	0.1464	2.172	0.0358	0.2213	0.4349	0.9929		0.2104	0.4027	0.0599	31.335	0.3528	0.17	0.4536	0.2471
	11	2	0	12.009	27.482	48.1449	25.2147	0	0	0	3.9904	0	3.3E-4
	12	1	0	6.38	20.55	57.92	14.86				5.25	0	0	0.2679	0.0362	0.2311	0.1939	0.0625	0.9023	0.0221	0.0751	0.1539	0.4281		0.0884	0.1666	0.0234	12.6223	0.1454	0.0664	0.187	0.1093
	12	2	0	7.1419	19.7681	58.9592	12.5052	0.6519	0	0	5.4311	0	0	0.2676	0.0252	0.3908	0.238	0.0492	1.3469	0.0205	0.1107	0.2002	0.6539		0.1451	0.2409	0.0387	18.5981	0.1753	0.0886	0.2503	0.164
	13	1	0									0	0
	13	2	0									0	0
	13	3	0									0	0
	14	1	0	0	42.7785	38.7635	52.629	0.2	0	3.0569	22.5483	0	0
	15	1	0	1.2968	20.5855	66.1482	13.3297	0	0	0	0	0	0
	15	1	0	2.6339	20.0303	64.136	13.2755	0	0	0	0	0	0
	15	1	0	0	24.3913	68.053	11.233	0	0	0	0	0	0
	16	1	0	9.0748	34.7441	63.5448	47.852	0	0	2.2375	0	0	0
	16	1	0	8.0345	22.5329	69.7153	13.3095	0	0	0	0	0	0
	17	1	46.0216	0	15.0631	28.461	1.8759	0	0	0.4409	0	0	0
	17	2	0	12	24.3049	72.0273	16.0508	0	0	0.829	0	0	0
	18	1	0	15	27.3369	67.663	21.1793	0	0	2.373	0	0	0
	18	2	0	6	18.4151	76.1756	7.3741	0	0	0	0	0	0
	19	1	0	0	36.3766	54.7955	28.4128	0.2536	0.5137	4.2414	0	0	0
	19	2	0	5.9321	46.843	63.0877	42.9206	0.1652	0.584	5.1828	0	0	0
												0	0

Identifier	experiment run	measurementnumber	pH	Dilution rate	Glucose	Ethanol	Acetate	Lactate	Formate	Succinate	Pyruvate	Acetoin	2,3-butanediol	O2	CO2	ATP (calculated)	PDH	Relative PDH flux	Serine	Glutamate	Glycine	Histidine	NH3	Arginine	Threonine	Alanine	Proline	Cysteine	Tyrosine	Valine	Methionine	Ornithine	Lysine	Isoluecine	Leucine	Phenylalanine	Yield glucose	Yield glucose	Comments
Unit	1	1	1	/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	%	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	g/mol	g/g
	1	1	6.5	0.05	-3.1494845360824746	1.7304123711340207	1.28659793814433	3.456701030927835	2.997938144329897	0.025	0	0.08659793814432991	0.002835051546391753	0	0	7.989175257731959	0.01907216494845354	0.0063215445070903585	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	15.875613747954171	0.08819785415530094	Old chemostat, however looks good
	2	1	6.5	0.05	-3.2500000000000004	1.1840425531914895	1.0287234042553193	4.336170212765957	2.5095744680851064	0.012765957446808512	0	0.050531914893617025	0.08244680851063832	0	0	7.869148936170213	-0.2968085106382974	-0.13413461538461513	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	15.384615384615383	0.08547008547008546	Looks good, little filaments
	3	1	6.5	0.15	-8.728571428571428	1.7514285714285713	1.15	16.001428571428573	3.15	0.06571428571428571	0.03	0.03571428571428571	0.19285714285714287	0	0	20.671428571428574	-0.24857142857142867	-0.08567208271787301	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	17.18494271685761	0.09547190398254227	Looks good, little filaments
	4	1	7.5	0.15	-23.499999999999996	1.869230769230769	1.2846153846153845	32.94230769230769	3.428846153846153	0.10576923076923077	0.3480769230769231	0	0.5134615384615384	0	0	38.96730769230769	-0.2749999999999999	-0.08719512195121949	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	6.382978723404255	0.03546099290780142	Looks good, little filaments
	5	1	7.5	0.05	-6.788888888888889	0.9511111111111112	0.6327777777777778	10.981111111111113	1.7202777777777778	0.04083333333333334	0.06194444444444444	0.01388888888888889	0.14916666666666667	0	0	13.667500000000004	-0.13638888888888867	-0.08611013679410719	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	7.364975450081833	0.04091653027823241	Looks good, little filaments
	6	1	6.5	0.15	-8.898058252427184	1.6762135922330097	1.4184466019417479	11.83252427184466	3.6830097087378637	0.020388349514563107	0	0	0.14563106796116504	0	0	16.677669902912623	-0.5883495145631064	-0.1901176470588234	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	16.857610474631752	0.09365339152573196	Looks good, no filaments att all
	7	1	7.5	0.15	-19.09375	1.1765625	0.39218750000000024	28.3890625	1.8640625	0.10625000000000001	0.13125	0	0.3390625	0	0	31.371875000000003	-0.29531249999999987	-0.18824701195219115	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	7.855973813420621	0.043644298963447896	Looks good, little filaments
	8	1	7.5	0.15	-22.912499999999998	3.804375	2.638125	24.16125	6.749999999999999	0	0.27749999999999997	0	0	0	0	33.519375000000004	-0.3074999999999992	-0.04772991850989511	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	6.546644844517186	0.03637024913620659	Looks good, no filaments att all
	8	2	6.5	0.15	-10.414772727272727	2.0105113636363634	1.8170454545454544	11.048011363636363	3.677556818181818	0	0.05113636363636363	0	0	0	0	16.74375	0.1499999999999999	0.03918949009129368	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	14.402618657937808	0.08001454809965448	Looks good, no filaments att all
	9	1	6	0.05	-3.6369047619047623	0.668452380952381	0.44761904761904764	4.829761904761906	1.306547619047619	0	0	0.05238095238095239	0.0892857142857143	0	0	6.676785714285716	-0.19047619047619047	-0.17066666666666666	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	13.747954173486088	0.07637752318603382	Looks good, no filaments att all
	10	1	6.5	0.15	-6.435586466165413	1.8584887218045112	2.2960037593984963	2.915627819548872	4.197394736842105	0	0	0	0	0	0	9.366124060150376	-0.04290225563909722	-0.01032671399291451	-0.27406015037593984	0.01105263157894734	-0.07206766917293231	-0.041165413533834565	0.5324436090225563	-0.2381954887218045	-0.04443609022556389	-0.016015037593984982	0.08954887218045116	0	-0.03609022556390977	-0.07026315789473679	-0.03631578947368421	0.020433834586466162	-0.006766917293233088	-0.07015037593984962	-0.0694736842105263	-0.007218045112781973	23.307899099579057	0.12948832833099477	High arginine, looks good, no filaments at all
	10	2	6.5	0.25	-12.794215246636771	2.299932735426009	2.9439461883408073	11.411076233183856	5.043789237668162	0	0	0	0	0	0	19.59890134529148	0.20008968609865452	0.03815680892092849	-0.6547085201793722	-0.1058295964125561	-0.15313901345291478	-0.11098654708520175	0.7847533632286995	-0.47892376681614346	-0.14573991031390132	-0.12937219730941704	-8.968609865472354E-4	0	-0.0751121076233184	-0.17959641255605374	-0.08094170403587442	0.03418116591928251	-0.07421524663677134	-0.12713004484304935	-0.14730941704035871	-0.05291479820627805	19.540080824083194	0.10855600457823997	High arginine, looks good, many filaments of around 4-8 cells
	10	3	6.5	0.4	-24.575027624309392	0	0.6106519337016577	29.43323756906077	1.7604419889502765	0	0	0	0	0	0	30.654541436464083	-1.1497900552486189	-1.8828894035900403	-1.2278453038674033	0.12110497237569053	0.010607734806629894	-0.1502762430939226	2.4614364640883974	-0.5851933701657459	-0.19093922651933698	-0.0751381215469614	0.49723756906077327	0	-0.031381215469613304	-0.07999999999999978	-0.12243093922651932	0.05829657458563536	0.0808839779005524	-0.07292817679558017	0.049060773480662984	0.036685082872928064	15.859886229412819	0.08811047905229344	High arginine, looks good, many filaments of around 2-4 cells
	11	1	6.5	0.05	-3.3314917127071824	0.6810276243093923	0.8181712707182319	2.5848729281767957	1.4138066298342542	0	0	0	-0.34254143646408847	0	0	4.217160220994474	0.08539226519337006	0.056958596672255835	0.0845303867403315	0.30441988950276244	0.20939226519337018	0.08088397790055249	1.2000000000000002	0.01977900552486188	0.12226519337016574	0.24027624309392265	0.5485635359116022	0	0.11624309392265195	0.22248618784530386	0.0330939226519337	0.0173121546961326	0.1949171270718232	0.09392265193370165	0.25060773480662984	0.13651933701657457	15.008291873963516	0.08337939929979732	High arginine, looks good, many filaments of around 10-20 cells
	11	2	6.5	0.05	-4.824	0.96072	1.17456	3.851592	2.017176	0	0	0	-0.176768	0	0	6.807896	0.11810399999999976	0.055310778914240646	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	10.364842454394692	0.05758245807997051	High arginine, looks good, many filaments of around 10-20 cells
	12	1	6.5	0.3	-15.705192660550455	1.7559633027522934	2.313908256880734	15.941284403669725	4.089908256880733	0	0	0	1.4449541284403669	0	0	25.21497247706422	-0.02003669724770596	-0.0049231768015579914	0.09963302752293576	0.6360550458715596	0.5336697247706421	0.17201834862385318	2.4833944954128437	0.060825688073394495	0.206697247706422	0.42357798165137617	1.1782568807339449	0	0.24330275229357798	0.45853211009174305	0.06440366972477063	0.03474027522935779	0.40018348623853206	0.18275229357798162	0.5146788990825688	0.30082568807339444	19.101962419955772	0.10612201344419873	High arginine, looks good, no filaments at all
	12	2	6.5	0.35	-15.54223346303502	1.9452645914396887	2.0769299610894945	16.05892607003891	3.40608560311284	0.1775603112840467	0	0	1.4792879377431905	0	0	25.471747081712067	0.616108949416343	0.1531773118803837	0.06863813229571984	1.0644357976653696	0.648249027237354	0.13400778210116732	3.6685992217898833	0.05583657587548639	0.3015175097276265	0.5452918287937742	1.7810505836575876	0	0.3952140077821012	0.656147859922179	0.10540856031128404	0.05065630350194552	0.4774708171206226	0.24132295719844354	0.681750972762646	0.44669260700389113	22.519285972149692	0.1251071442897205	High arginine, looks good, many filaments of around 4-8 cells
	13	1	6.5	0.15	-11.38509316770186	0	-2.273291925465838	0	0	0	0	0	0	0	0	-4.546583850931676	-2.273291925465838	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	13.175122749590836	0.07319512638661575	Very high arginine, looks good, almost no filaments
	13	2	6.5	0.35													0																				0	0	Culture showed very low density. Stopped pump and set te D=0.15
	13	3	6.5	0.15													0																				0	0	0
	14	1	7.5	0.05	-8.369863013698788	0	4.1888356164384355	5.310068493150785	7.209452054794657	0.027397260273973125	0	0.4187534246575422	3.0888082191781407	0	0	20.757657534246967	-3.020616438356221		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	5.973813420621818	0.03318785233678788	No glucose, culture look OK, many filaments
	15	1	7.5	0.12	-18.33000000000202	0.3890400000000428	2.515650000000277	19.844460000002186	3.9989100000004405	0	0	0	0	0	0	25.264800000002783	-1.0942200000001208		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	6.546644844516464	0.03637024913620258	Culture looked OK
	15	1	7.5	0.12	-14.2368932038823	0.6137242718446088	1.8245359223299442	14.944310679610394	3.093320388349255	0	0	0	0	0	0	19.207106796114893	-0.6550601941747018		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	8.428805237316583	0.04682669576286991	Culture looked OK
	15	1	7.5	0.12	-17.882926829263482	0	3.568185365852699	19.917951219506836	3.2877073170722864	0	0	0	0	0	0	27.054321951212234	0.2804780487804126		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	6.71031096563192	0.037279505364621775	Culture looked OK
	16	1	6.5	0.12	-6.94976303317517	1.0322047393364653	2.564257819905145	7.227844549762841	5.442881516587533	0	0	0.25450236966823964	0	0	0	13.897569668246074	-1.8464189573459224		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	17.266775777414537	0.09592653209674742	Culture looked OK
	16	1	6.5	0.12	-6.726605504587486	0.8845321100917863	1.1375669724771202	7.675078899082945	1.4652660550459433	0	0	0	0	0	0	10.834744954128972	0.5568330275229634		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	17.839607201308453	0.09910892889615808	Culture looked OK
	17	1	7.5	0.15	-15.598344827593898	0	2.961827586208358	29.44241379311796	1.9405862068975084	0	0	0.4561034482760869	0	0	0	36.27827586208685	1.0212413793108495																				2.3731587561363097	0.013184215311868388	OD and dryweight too low, found residual glucose in HPLC sample
	17	2	7.5	0.05	-9.257575757575758	1.8181818181818183	1.8340757575757576	10.913227272727273	2.431939393939394	0	0	0.1256060606060606	0	0	0	16.650772727272727	1.220318181818182																				5.400981996726678	0.030005455537370433	Dryweight estimated from OD
	18	1	6.5	0.35	-20.35567692307692	4.038461538461538	3.9861499999999994	18.216961538461536	5.70211923076923	0	0	0.5131807692307693	0	0	0	31.254084615384613	2.3224923076923085																				17.19422062565801	0.09552344792032227	Culture looked OK
	18	2	6.5	0.35	-24.056709090909088	1.9090909090909085	1.8721499999999995	24.237690909090905	2.346304545454545	0	0	-0.14855909090909086	0	0	0	29.59396363636363	1.4349363636363628																				14.548955914018316	0.08082753285565732	Culture looked OK
	19	1	6.5	0.1	-6.751381215470109	0	2.6714475138123515	6.0547513812159135	3.1395359116024406	0.028022099447515866	0.05676243093923069	0.4686629834254487	0	0	0	12.447779005525776	-0.46808839779008915		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0			Culture looked OK
	19	2	6.5	0.1	-5.791469194312642	0.5622843601895585	3.2836966824643676	5.979876777251025	4.068303317535436	0.015658767772511434	0.05535545023696535	0.49126066350709596	0	0	0	14.178748815165498	-0.22232227488151013		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0			Culture looked OK
	0	0	0	0																																			0
	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0			0
	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0			0
	0	0	0	0																																			0
	0	0	0	0																																			0
	0	0	0	0																																			0
	0	0	0	0																																			0
	0	0	0	0																																			0
	0	0	0	0																																			0

Identifier	experiment run	measurementnumber	pH	Dilution rate	Ethanol	Acetate	Lactate	Formate	Succinate	Pyruvate	Acetoin	2,3-butanediol	Dryweight
Unit	1	1	1	/hour	mol/mol	mol/mol	mol/mol	mol/mol	mol/mol	mol/mol	mol/mol	mol/mol	g/l
	1	1	6.5	0.05	0.5494271685761047	0.4085106382978723	1.097545008183306	0.9518821603927986	0.007937806873977086	0	0.027495908346972176	9.00163666121113E-4	0.97
	2	1	6.5	0.05	0.36432078559738135	0.31653027823240587	1.3342062193126019	0.7721767594108019	0.003927986906710311	0	0.015548281505728314	0.025368248772504095	0.94
	3	1	6.5	0.15	0.20065466448445174	0.13175122749590834	1.833224222585925	0.36088379705400986	0.007528641571194763	0.0034369885433715225	0.004091653027823241	0.022094926350245504	1.05
	4	1	7.5	0.15	0.0795417348608838	0.054664484451718495	1.4018003273322426	0.14590834697217675	0.004500818330605566	0.014811783960720136	0	0.021849427168576106	0.39
	5	1	7.5	0.05	0.1400981996726678	0.09320785597381342	1.6175122749590838	0.2533960720130933	0.006014729950900165	0.009124386252045827	0.0020458265139116204	0.0219721767594108	0.45
	6	1	6.5	0.15	0.188379705400982	0.1594108019639935	1.3297872340425532	0.413911620294599	0.002291325695581015	0	0	0.016366612111292964	1.03
	7	1	7.5	0.15	0.061620294599018	0.02054009819967268	1.4868248772504093	0.09762684124386252	0.005564648117839608	0.006873977086743045	0	0.017757774140752865	0.48
	8	1	7.5	0.15	0.1660392798690671	0.115139116202946	1.0545008183306057	0.2945990180032733	0	0.012111292962356792	0	0	0.4
	8	2	6.5	0.15	0.19304418985270047	0.17446808510638298	1.0608019639934534	0.35310965630114566	0	0.004909983633387889	0	0	0.88
	9	1	6	0.05	0.18379705400981997	0.12307692307692307	1.3279869067103112	0.3592471358428805	0	0	0.014402618657937807	0.024549918166939445	0.84
	10	1	6.5	0.15	0.28878311737016804	0.35676682637542895	0.45304772686647204	0.6522163533827998	0	0	0	0	1.33
	10	2	6.5	0.25	0.17976348616071586	0.2300997858477241	0.8918934075447494	0.3942241974547074	0	0	0	0	1.115
	10	3	6.5	0.4	0	0.024848473948310294	1.1976888904875809	0.0716354022409669	0	0	0	0	0.905
	11	1	6.5	0.05	0.20442122719734662	0.24558706467661687	0.7758905472636817	0.42437645107794364	0	0	0	-0.10281923714759537	0.905
	11	2	6.5	0.05	0.1991542288557214	0.24348258706467663	0.7984228855721394	0.4181542288557214	0	0	0	-0.036643449419568826	0.625
	12	1	6.5	0.3	0.11180781673331909	0.14733396188720382	1.0150327186824204	0.2604175794133419	0	0	0	0.09200486486675945	1.09
	12	2	6.5	0.35	0.1251599132175065	0.13363137067971442	1.033244424505189	0.21915033069177142	0.011424375506026757	0	0	0.09517859458625849	1.285
	13	1	6.5	0.15	0	-0.19967266775777415	0	0	0	0	0	0	0.805
	13	2	6.5	0.35									0
	13	3	6.5	0.15									0
	14	1	7.5	0.05	0	0.5004664484451719	0.6344271685761047	0.8613584288052373	0.003273322422258593	0	0.05003109656301146	0.36903927986906715	0.3649999999999931
	15	1	7.5	0.12	0.02122422258592471	0.13724222585924714	1.0826219312602292	0.2181620294599018	0	0	0	0	0.39999999999995595
	15	1	7.5	0.12	0.04310801963993454	0.12815548281505731	1.0496890343698853	0.21727495908346972	0	0	0	0	0.5150000000000432
	15	1	7.5	0.12	0	0.1995302782324059	1.1137970540098199	0.18384615384615385	0	0	0	0	0.4100000000001103
	16	1	6.5	0.12	0.14852373158756135	0.3689705400981997	1.0400130932896892	0.7831751227495909	0	0	0.036620294599018	0	1.0550000000000281
	16	1	6.5	0.12	0.13149754500818328	0.16911456628477908	1.1410032733224222	0.21783142389525367	0	0	0	0	1.0899999999999466
	17	1	7.5	0.15	0	0.18988088921901533	1.8875344864176566	0.12440975169779285	0	0	0.029240502971137516	0	0.14499999999992852
	17	2	7.5	0.05	0.19639934533551556	0.19811620294599017	1.1788428805237317	0.26269721767594106	0	0	0.013567921440261865	0	0.33
	18	1	6.5	0.35	0.19839485337297705	0.19582497870561907	0.894932730918383	0.28012427453615285	0	0	0.025210695334282107	0	1.3
	18	2	6.5	0.35	0.0793579413491908	0.07782236518408396	1.0075231328399032	0.09753223255051133	0	0	-0.006175370469322864	0	1.1
	19	1	6.5	0.1	0	0.39568903436988556	0.8968166939443535	0.4650212765957447	0.004150572831423895	0.008407528641571195	0.06941734860883797	0	0.9049999999999336
	19	2	6.5	0.1	0.09708837970540099	0.5669885433715222	1.032531914893617	0.7024648117839607	0.0027037643207855977	0.00955810147299509	0.08482487725040917	0	1.0550000000000281