Characterization of flux distribution and general physiology of E. faecalis in continuous cultures

Selected Cell

Cell:

Value:

Metadata Environmental parameters Measurements environ parameters Medium composition (calculated) Organic acids concentrations Calculated fluxes

Item	Value
Experimentator	Maria/ Ibrahim
Raw data
Reference	SYSMO-LAB

Description	Growth in glucose-limited anaerobic chemostat conditions
Aim	Effect of dilution rate of pyruvate branching
Strain information	E. faecalis V583
Strain	Enterococus faecalis
Vessel type	Sartorius fermentor
Stirring rate	250	RPM
Temperature	37	degree celcius
Dilution rate	Variable	/h
Volume	750	ml
Gas flow		ml
pH	Variable
SOP's used
Jermtype	1

	experiment run	measurementnumber	Age	Date	Chemostat	Medium flow	Volume	Temperature	pH Chemostat	Dilution rate	%O2 input	Airflow	N2 flow	Total flow	Comments
Unit	1	1	hours	Date	name	ml/hour	ml	Celcius	1	1/hour	volume%	ml/min	ml/min	ml/min	1
	1	1	96	2009-04-29T0:0:0+0000	Kjell	37.5	750	37	6.4	0.05	0	0	10 on scale
	1	1	120	2009-04-30T0:0:0+0000	Kjell	37.5	750	37	6.4	0.05	0	0	10 on scale
	2	2	96	2009-06-15T0:0:0+0000	Kjell	37.5	750	37	6.65	0.05	0	0	10 on scale
	2	2	120	2009-06-16T0:0:0+0000	Kjell	37.5	750	37	6.42	0.05	0	0	10 on scale
	3	2	96	2009-08-28T0:0:0+0000	Kjell	37.5	750	37	6.4	0.05	0	0	10 on scale
	4	2	96	2009-09-08T0:0:0+0000	Kjell	37.5	750	37	6.4	0.05	0	0	10 on scale
	4	2	120	2009-09-09T0:0:0+0000	Kjell	37.5	750	37	6.4	0.05	0	0	10 on scale
	5	2	96	2009-06-10T0:0:0+0000	Kjell	37.5	750	37	7.43	0.05	0	0	10 on scale
	5	2	120	2009-06-11T0:0:0+0000	Kjell	37.5	750	37	7.45	0.05	0	0	10 on scale


	6	2	72	2008-12-04T0:0:0+0000	Kjell	112	750	37	6.4	0.15	0	0	10 on scale
	7	2	72	2009-01-13T0:0:0+0000	Kjell	112	750	37	6.4	0.15	0	0	10 on scale
	7	2	96	2009-12-14T0:0:0+0000	Kjell	112	750	37	6.4	0.15	0	0	10 on scale
	8	2	72	2009-05-10T0:0:0+0000	Kjell	112	750	37	6.4	0.15	0	0	10 on scale
	8	2	96	2009-05-11T0:0:0+0000	Kjell	112	750	37	6.4	0.15	0	0	10 on scale
	9	2	72	2009-06-19T0:0:0+0000	Kjell	112	750	37	6.45	0.15	0	0	10 on scale
	9	2	96	2009-06-20T0:0:0+0000	Kjell	112	750	37	6.4	0.15	0	0	10 on scale
	10	2	72	2009-08-22T0:0:0+0000	Kjell	112	750	37	6.4	0.15	0	0	10 on scale
	11	2	72	2009-06-22T0:0:0+0000	Kjell	112	750	37	7.4	0.15	0	0	10 on scale
	11	2	84	2009-06-22T0:0:0+0000	Kjell	112	750	37	7.42	0.15	0	0	10 on scale


	12	2		2009-05-01T0:0:0+0000	Kjell	300	750	37	6.4	0.4	0	0	10 on scale
	12	2		2009-05-02T0:0:0+0000	Kjell	300	750	37	6.4	0.4	0	0	10 on scale
	13	2		2009-02-09T0:0:0+0000	Kjell	300	750	37	6.4	0.4	0	0	10 on scale
	13	2		2009-02-10T0:0:0+0000	Kjell	300	750	37	6.4	0.4	0	0	10 on scale
	14	2		2009-06-24T0:0:0+0000	Kjell	300	750	37	6.4	0.4	0	0	10 on scale
	14	2		2009-06-24T0:0:0+0000	Kjell	300	750	37	6.39	0.4	0	0	10 on scale
	15	2		2009-08-29T0:0:0+0000	Kjell	300	750	37	6.4	0.4	0	0	10 on scale
	16	2		2009-06-22T0:0:0+0000	Kjell	300	750	37	7.39	0.4	0	0	10 on scale
	16	2		2009-06-23T0:0:0+0000	Kjell	300	750	37	7.4	0.4	0	0	10 on scale
	17	2		2009-06-24T0:0:0+0000	Kjell	300	750	37	6.4	0.4	0	0	10 on scale
	17	2		2009-06-24T0:0:0+0000	Kjell	300	750	37	6.39	0.4	0	0	10 on scale
	18	2		2009-08-29T0:0:0+0000	Kjell	300	750	37	6.4	0.4	0	0	10 on scale
	19	2		2009-09-25T0:0:0+0000	Kjell	300	750	37	6.4	0.4	0	0	10 on scale
	19	2		2009-09-25T0:0:0+0000	Kjell	300	750	37	6.4	0.4	0	0	10 on scale


	20	2		2008-11-29T0:0:0+0000	Kjell	37.5	0.05	37	7.4	0.05	0	0	10 on scale
	20	2		2008-11-30T0:0:0+0000	Kjell	37.5	0.05	37	7.4	0.05	0	0	10 on scale
	21	2		2009-05-07T0:0:0+0000	Kjell	37.5	0.05	37	7.4	0.05	0	0	10 on scale
	21	2		2009-05-08T0:0:0+0000	Kjell	37.5	0.05	37	7.4	0.05	0	0	10 on scale
	22	2		2009-06-10T0:0:0+0000	Kjell	37.5	0.05	37	7.43	0.05	0	0	10 on scale
	22	2		2009-06-11T0:0:0+0000	Kjell	37.5	0.05	37	7.45	0.05	0	0	10 on scale


	23	2		2008-08-21T0:0:0+0000	Kjell	112	0.15	37	7.3	0.15	0	0	10 on scale
	23	2		2008-08-22T0:0:0+0000	Kjell	112	0.15	37	7.3	0.15	0	0	10 on scale
	24	2		2008-09-12T0:0:0+0000	Kjell	112	0.15	37	7.3	0.15	0	0	10 on scale
	25	2		2009-06-22T0:0:0+0000	Kjell	112	0.15	37	7.4	0.15	0	0	10 on scale
	25	2		2009-06-22T0:0:0+0000	Kjell	112	0.15	37	7.42	0.15	0	0	10 on scale



	26	2		2009-02-12T0:0:0+0000	Kjell	300	0.4	37	7.4	0.4	0	0	10 on scale
	26	2		2009-02-13T0:0:0+0000	Kjell	300	0.4	37	7.4	0.4	0	0	10 on scale
	27	2		2009-06-23T0:0:0+0000	Kjell	300	0.4	37	7.39	0.4	0	0	10 on scale
	27	2		2009-06-24T0:0:0+0000	Kjell	300	0.4	37	7.4	0.4	0	0	10 on scale




	28	2		2009-02-18T0:0:0+0000	Kjell	37.5	0.05	37	6.4	0.05	0	0	10 on scale	a
	28	2		2009-02-19T0:0:0+0000	Kjell	37.5	0.05	37	6.4	0.05	0	0	10 on scale	a
	29	2		2009-03-31T0:0:0+0000	Kjell	37.5	0.05	37	6.4	0.05	0	0	10 on scale	a
	29	2		2009-04-01T0:0:0+0000	Kjell	37.5	0.05	37	6.4	0.05	0	0	10 on scale	a
	30	2		2009-03-29T0:0:0+0000	Kjell	300	0.4	37	6.4	0.4	0	0	10 on scale	a
	30	2		2009-03-30T0:0:0+0000	Kjell	300	0.4		6.4	0.4	0	0	10 on scale	a

	31	2		2009-02-24T0:0:0+0000	Kjell	37.5	0.05	37	7.4	0.05	0	0	10 on scale	a
	31	2		2009-02-25T0:0:0+0000	Kjell	37.5	0.05	37	7.4	0.05	0	0	10 on scale	a

Identifier	experiment run	measurementnumber	O2	O2	CO2	pH	RPM	OD	Dry weight	standard deviation dryweight
Unit	1	1	volume%	volume%	volume%	1	rpm	optical density (600 nm)	gram/liter	gram/liter
Description			Inputgas	Outputgas	Outputgas		stirring rate		Dryweight measured as described in SOP X
	1	2	?	?	?	6.4	250	2.7	1.4
	1	2	?	?	?	6.4	250	2.6	1.4
	2	2	?	?	?	6.4	250		1.14
	2	2	?	?	?	6.4	250		1.32
	3	2	?	?	?	6.4	250	2.4	1.46
	4	2	?	?	?	6.4	250	2.4	1.34
	4	2	?	?	?	6.4	250	2.4	1.38
	5	2	?	?	?	7.43	250	2.4	1.37
	5	2	?	?	?	7.45	250	2.4	1.61


	6	2	?	?	?	6.4	250	2.9	1.46
	7	2	?	?	?	6.4	250	2.5	1.57
	7	2	?	?	?	6.4	250	2.4	1.8
	8	2	?	?	?	6.4	250	2.4	1.45
	8	2	?	?	?	6.4	250	2.5	1.46
	9	2	?	?	?	6.45	250	2.7	1.58
	9	2	?	?	?	6.4	250	2.7	1.73
	10	2	?	?	?	6.4	250	2.7	1.71
	11	2	?	?	?	7.4	250	3	2.14
	11	2	?	?	?	7.42	250	3	2.18


	12	2	?	?	?	6.4	250	2.6	1.68
	12	2	?	?	?	6.4	250	2.6	1.66
	13	2	?	?	?	6.4	250	3	2.06
	13	2	?	?	?	6.4	250	3	2.01
	14	2	?	?	?	6.4	250	3	2.2
	14	2	?	?	?	6.39	250	3.1	2.25
	15	2	?	?	?	6.4	250	2.9	1.7
	16	2	?	?	?	7.39	250	2.7	2.1
	16	2	?	?	?	7.4	250	2.8	2.05
	17	2	?	?	?	6.4	250	3	2.2
	17	2	?	?	?	6.39	250	3.1	2.25
	18	2	?	?	?	6.4	250	2.9	1.7
	19	2	?	?	?	6.4	250	2.7	1.6
	19	2	?	?	?	6.4	250	2.8	1.71
	Average
	St. Dev.
	20	2	?	?	?		250	2.9	1.63
	20	2	?	?	?		250	3	1.73
	21	2	?	?	?		250	2.9	1.975
	21	2	?	?	?		250	3	2.025
	22	2	?	?	?		250	2.4	1.37
	22	2	?	?	?		250	2.4	1.61
	Average								1.7233333333333334
	St. Dev.								0.24526856028987296
	23	2	?	?	?		250	2.7	2.03
	23	2	?	?	?		250	2.7	2.03
	24	2	?	?	?		250	2.7	1.75
	25	2	?	?	?		250	3	2.14
	25	2	?	?	?		250	3	2.18
	Average								2.026
	St. Dev.								0.1680178561939196
	26	2	?	?	?		250		2.2
	26	2	?	?	?		250		2.5
	27	2	?	?	?		250	2.7	2.1
	27	2	?	?	?		250	2.8	2.05
	Average								2.2125000000000004
	St. Dev.								0.2015564437074602

	28	2	?	?	?		250	4	2.7
	28	2	?	?	?		250	4.4	2.65
	29	2	?	?	?		250	3.8	2.22
	29	2	?	?	?		250	3.4	2.195
	30	2	?	?	?		250	3	1.49
	30	2	?	?	?		250	3	1.71
	Average
	St. Dev.
	31	2	?	?	?		250	4.9	2.75
	31	2	?	?	?		250	4.9	2.8

Identifier

Experiment

measurementnumber

Glucose

Ethanol

Acetate

Lactate

Formiate

Succinate

Pyruvate

Acetoin

2,3 Butanediol

Unit

61.05

12.2

Identifier	Experiment	measurementnumber	Glucose	Ethanol	Acetate	Lactate	Formiate	Succinate	Pyruvate	Acetoin	2,3 Butanediol	Phosphate
Unit	1	1	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM
	1	2	0	36.3965	52.816649999999996	28.7476	68.18605	0	0.5358499999999999	0	0	36.01675
	1	2	0	31.7835	51.388	30.16435	66.3036	0	0.7362	0	0	35.12265
	2	2	0	14.46	36.57715	46.481049999999996	32.3784	0	0	0	0	34.45125
	2	2	0	23.808950000000003	43.0162	38.3166	49.7411	0	0	0	0	33.9932
	3	2	0	12.0189	52.617149999999995	16.9373	56.31375	0	0.6221	0	0	33.189899999999994
	4	2	0	12.805	27.605	9.86	29.895	0	0.76	0	0	19.075
	4	2	0	12.74	27.65	9.87	29.765	0	0.77	0	0	19.200000000000003
	5	2	0	29.74375	46.5727	15.38275	63.784949999999995	0	0	0	0	33.217
	5	2	0	33.40475	58.8123	14.8157	71.34545	0	0	0	0	34.0078
	6	2	0	22.5	37.1254	43.169250000000005	44.17355	0	0	0	0	34.9532
	7	2	0	28.57275	38.2931	43.4388	46.042500000000004	0	0	0	0	32.17
	7	2	0	32.24135	44.595	37.1472	56.17425	0	0.28995	0	0	31.01
	8	2	0	21.30105	25.3667	53.0481	38.800250000000005	0	0	0	0	35.97965000000001
	8	2	0	21.558349999999997	25.32465	53.137550000000005	38.88375	0	0.1254	0	0	35.9005
	9	2	0
	9	2	0
	10	2	0	17.8645	36.95075	38.3445	35.1359	0	0.59915	0	0	33.63315
	11	2	0	25.5697	39.56415	11.01265	56.72465	0	1.99505	0	0	28.59705
	11	2	0	29.46575	43.52855	12.1509	60.9166	0	2.05465	0	0	66.01644999999999

	12	2	0	11.074300000000001	25.632550000000002	68.281	19.9252	0	0.49115	0	0	37.266450000000006
	12	2	0	9.8742	25.2536	65.94555	19.94215	0	0.66855	0	0	36.54235
	13	2	0	13.9353	31.4328	59.73435	29.518549999999998	0	0.1489	0	0.4144	34.9142
	13	2	0	17.3673	34.84015	59.095600000000005	35.0616	0	0.56045	0	0.22815	36.282399999999996
	14	2	3.9741	7.34295	22.2106	84.286	15.35125	0	0	0	0	33.2382
	14	2	4.7578499999999995	7.25925	23.88895	111.0941	14.77355	0	0	0	0	38.0927
	15	2	0	27.22185	31.2571	49.46245	25.70295	0	0.98255	0	0	34.6868
	16	2	0	23.89875	42.815	17.80245	50.02265	0	1.24635	0	0	48.2198
	16	2	0	19.67755	36.26865	16.4579	40.6055	0	0	0	0	28.9909
	17	2	3.9741	7.34295	22.2106	84.286	15.35125	0	0	0	0	33.238200000000006	Note. Not steady state, since residual glucose.
	17	2	4.757849999999999	7.25925	23.88895	111.0941	14.77355	0	0	0	0	38.0927
	18	2	0	27.22185	31.2571	49.462450000000004	25.70295	0	0.98255	0	0	34.6868
	19	2	0	5.625	19.665	35.345	15.815	0	0.41	0	0	23.135
	19	2	0	5.895	19.51	35.504999999999995	15.62	0	0.38	0	0	23.1

	20	2	0	43.11965	62.448750000000004	4.128	85.99445	0	0.1475	0	0	32.3522
	20	2	0	18.3539	53.35495	7.8252500000000005	73.46795	0	0.1255	0.9716	0
	21	2	0	35.051500000000004	56.07985	11.733350000000002	76.90035	0	0.15639999999999998	0	0	35.3481
	21	2	0	39.782349999999994	60.84	6.7533	84.2443	0	0.1347	0	0	35.512249999999995
	22	2	0	29.74375	46.5727	15.382750000000001	63.784949999999995	0	0	0	0	33.217
	22	2	0	33.40475	58.8123	14.8157	71.34545	0	0	0	0	34.0078

											0	0
	23	2	0	33.615	60.505	6.715	73.8	0	0	0	0	0
	23	2	0	37.215	62.085	9.655000000000001	78.555	0	0	0	0	0
	24	2	0	35.349999999999994	53.92	13.625	70.545	0	0	0	0	0
	25	2	0	25.569699999999997	39.56415	11.01265	56.72465	0	1.99505	0	0	28.59705
	25	2	0	29.46575	43.52855	12.1509	60.9166	0	2.05465	0	0	66.01644999999999



	26	2	0	26.027549999999998	43.206100000000006	36.9628	53.2111	0	0.47635000000000005	0	0.3651	32.551100000000005
	26	2	0	27.779200000000003	46.05685	38.61895	57.67885	0	0.2517	0	0	32.37595
	27	2	0	23.89875	42.815	17.80245	50.02265	0	1.24635	0	0	48.2198
	27	2	0	19.67755	36.26865	16.4579	40.605500000000006	0	0	0	0	28.9909

	28	2	0	8.66965	94.35294999999999	2.9002499999999998	8.6917	0.7926	0	1.4055499999999999	1.11765	0
	28	2	0	3.72395	97.08135	2.2123	8.21145	0.81015	0	1.3473000000000002	1.0285	0.81015		34.513
	29	2	0	7.0099	49.37075	49.70805	21.5004	0	0.6327499999999999	0	0	32.85955		33.41075
	29	2	0	4.41775	32.62395	63.5631	9.399650000000001	0	1.3886	1.6913	0	34.88665
	30	2	0	7.01	49.370000000000005	49.71	21.494999999999997	0	0.635	0	0	32.855000000000004
	30	2	0	4.415	32.620000000000005	63.565	9.4	0	1.385	0		34.89

	31	2	0	5.8652	88.58160000000001	4.4937000000000005	25.164450000000002	0.9235	0	1.34295	0.7169	32.291650000000004
	31	2	0	7.0377	86.57589999999999	5.67655	23.6679	0.9333	0	1.5341	0.9929	35.20865

Identifier	experiment run	measurementnumber	Glucose	Ethanol	Acetate	Lactate	Formate	CO2	O2	Succinate	Pyruvate	Acetoin	2,3-butanediol	Carbon balance	ATP (calculated)
Unit	1	1	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	%	mmol/gram/hour
	1	2	2.178571428571429	1.2998750000000003	1.8863089285714287	1.0267000000000002	2.4352160714285715	0	0	0	0.019137499999999998	0	0
	1	2	2.178571428571429	1.1351250000000002	1.8352857142857144	1.0772982142857144	2.3679857142857146	0	0	0	0.026292857142857146	0	0
	2	2	2.6754385964912286	0.634210526315789	1.6042609649122814	2.0386425438596483	1.420105263157895	0	0	0	0	0	0
	2	2	2.3106060606060606	0.901854166666667	1.629401515151515	1.451386363636364	1.8841325757575762	0	0	0	0	0	0
	3	2	1.46	2.089041095890411	0.411606164383562	1.8019571917808221	0.580044520547945	1.9285530821917811	0	0	0	0.0213047945205479	0
	4	2	2.2761194029850746	0.47779850746268654	1.030037313432836	0.367910447761194	1.1154850746268656	0	0	0	0.028358208955223882	0	0
	4	2	2.2101449275362324	0.46159420289855074	1.0018115942028987	0.35760869565217396	1.0784420289855075	0	0	0	0.027898550724637688	0	0
	5	2	2.2761194029850746	0.47779850746268654	1.030037313432836	0.367910447761194	1.1154850746268656	0	0	0	0.028358208955223882	0	0
	5	2	2.2101449275362324	0.46159420289855074	1.0018115942028987	0.35760869565217396	1.0784420289855075	0	0	0	0.027898550724637688	0	0


	6	2	6.267123287671232	2.3116438356164375	3.8142534246575335	4.43519691780822	4.538378424657536	0	0	0	0	0	0
	7	2	5.83	2.729880573248407	3.658576433121019	4.15020382165605	4.398964968152867	0	0	0	0	0	0
	7	2	5.083333333333333	2.6867791666666663	3.7162499999999996	3.0955999999999997	4.681187499999999	0	0	0	0	0	0
	8	2	6.267123287671233	2.1884640410958904	2.606167808219178	5.4501472602739724	3.986327054794521	0	0	0	0	0	0
	8	2	6.267123287671233	2.2148989726027395	2.6018476027397255	5.459337328767124	3.994905821917808	0	0	0	0.012883561643835617	0	0
	9	2	5.791139240506329	1.858770569620253	2.514588607594937	2.793151898734177	3.6498797468354427	0	0	0	0	0	0
	9	2	5.289017341040463	1.5442543352601157	2.1001127167630056	2.7074956647398842	3.0263106936416193	0	0	0	0.012883561643835617	0	0
	10	2	5.350877192982456	1.567061403508772	3.241293859649123	3.363552631578947	3.08209649122807	0	0	0	0.0525570175438596	0	0
	11	2	4.275700934579439	1.7922686915887853	2.773188084112149	0.771914719626168	3.9760268691588765	0	0	0	0.139839953271028	0	0
	11	2	4.197247706422019	2.0274598623853213	2.99508371559633	0.836071100917431	4.191509174311926	0	0	0	0.141375	0	0


	12	2	14.523809523809526	2.636738095238096	6.102988095238096	16.257380952380956	4.744095238095238	0	0	0	0.11694047619047619	0	0
	12	2	14.698795180722893	2.3793253012048194	6.085204819277108	15.890493975903615	4.8053373493975915	0	0	0	0.16109638554216868	0	0
	13	2	11.844660194174757	2.705883495145631	6.103456310679612	11.598902912621359	5.731757281553398	0	0	0	0.0289126213592233	0	0
	13	2	12.139303482587067	3.4561791044776125	6.933363184079603	11.760318407960202	6.977432835820896	0	0	0	0.11153233830845773	0	0
	14	2	10.434343434343434	1.335081818181818	4.038290909090909	15.324727272727273	2.7911363636363635	0	0	0		0	0
	14	2	10.074074074074074	1.2905333333333333	4.246924444444445	19.750062222222223	2.6264088888888892	0	0	0		0	0
	15	2	14.787878787878787	6.599236363636365	7.577478787878789	11.990896969696971	6.231018181818183	0	0	0	0.238193939393939	0	0
	16	2	11.619047619047622	4.552142857142856	8.155238095238097	3.390942857142857	9.52812380952381	0	0	0	0.2374	0	0
	16	2	11.902439024390253	3.8395219512195133	7.076809756097562	3.21129756097561	7.9230243902439055	0	0	0	0	0	0
	17	2	10.434343434343429	1.335081818181818	4.038290909090909	15.324727272727271	2.7911363636363635	0	0	0		0	0
	17	2	10.074074074074069	1.290533333333333	4.246924444444445	19.75006222222222	2.6264088888888883	0	0	0		0	0
	18	2	14.78787878787879	6.599236363636364	7.577478787878789	11.990896969696973	6.2310181818181825	0	0	0	0.23819393939393943	0	0
	19	2	15.25	1.40625	4.91625	8.836250000000001	3.95375	0	0	0	0.1025	0	0
	19	2	14.26900584795322	1.3789473684210534	4.563742690058479	8.305263157894736	3.653801169590644	0	0	0	0.0888888888888889	0	0


	20	2	1.8711656441717794	1.3226886503067485	1.9156058282208592	0.12662576687116567	2.6378665644171786	0	0	0	0	0	0
	20	2	1.7630057803468209	0.5304595375722544	1.5420505780346823	0.2261632947976879	2.123351156069364	0	0	0	0	0	0
	21	2	1.5404040404040404	0.885138888888889	1.4161578282828284	0.29629671717171724	1.9419280303030306	0	0	0	0	0	0
	21	2	1.5024630541871924	0.9798608374384237	1.498522167487685	0.16633743842364537	2.0749827586206897	0	0	0	0	0	0
	22	2	2.2262773722627736	1.085538321167883	1.6997335766423352	0.561414233576642	2.327917883211679	0	0	0	0	0	0
	22	2	1.8944099378881991	1.037414596273292	1.826468944099379	0.460114906832298	2.215697204968944	0	0	0	0	0	0
	Average		1.7996209715434677	0.9735168052745818	1.6497564871279613	0.30615872627885937	2.220290599598481	0	0	0	0	0	0
	St dev.		0.2656976439715548	0.26180273886777367	0.19669346190946096	0.1715438625361542	0.24247546770449707	0	0	0	0	0	0
	23	2	4.507389162561577	2.483866995073892	4.4708128078817735	0.49618226600985227	5.45320197044335	0	0	0	0	0	0
	23	2	4.507389162561577	2.7498768472906407	4.5875615763546795	0.713423645320197	5.804556650246306	0	0	0	0	0	0
	24	2	5.228571428571429	3.03	4.621714285714285	1.1678571428571427	6.046714285714286	0	0	0	0	0	0
	25	2	4.275700934579439	1.7922686915887847	2.773188084112149	0.7719147196261682	3.9760268691588774	0	0	0	0.13983995327102802	0	0
	25	2	4.197247706422019	2.027459862385321	2.99508371559633	0.8360711009174311	4.191509174311926	0	0	0	0.141375	0	0
	Average		4.543259678939209	2.4166944792677274	3.8896720939318437	0.7970897749461583	5.09440178997495	0	0	0	0	0	0
	St dev.		0.40728907083390137	0.5082020883221159	0.9229687075508458	0.24349903412196683	0.9494648655350025	0	0	0	0	0	0

	26	2	11.090909090909092	4.732281818181818	7.855654545454547	6.720509090909091	9.674745454545455	0	0	0	0.08660909090909093	0	0
	26	2	9.760000000000002	4.444672000000001	7.369096000000001	6.179032	9.228615999999999	0	0	0	0.040271999999999995	0	0
	27	2	11.619047619047622	4.552142857142856	8.155238095238097	3.390942857142857	9.52812380952381	0	0	0	0.2374	0	0
	27	2	11.902439024390253	3.8395219512195133	7.076809756097562	3.21129756097561	7.9230243902439055	0	0	0	0	0	0
	Average		11.093098933586742	4.3921546566360465	7.614199599197551	4.87544537725689	9.088627413578292	0	0	0	0	0	0
	St dev.		0.9502299659312216	0.3870588623506782	0.4830068195089483	1.8327332845679334	0.798940684559012	0	0	0	0	0	0


	28	2	1.12962962962963	0.1605490740740741	1.7472768518518518	0.05370833333333332	0.1609574074074074	0	0	0.014677777777777776	0	0.0260287037037037	0.02069722222222222	In presence of Oxygen
	28	2	1.1509433962264153	0.07026320754716982	1.8317235849056606	0.041741509433962265	0.15493301886792454	0	0	0.015285849056603774	0	0.025420754716981134	0.01940566037735849	In presence of Oxygen
	29	2	1.3863636363636365	0.1593159090909091	1.1220625	1.129728409090909	0.48864545454545455	0	0	0	0.014380681818181817	0.03843863636363636	0	In presence of Oxygen
	29	2	1.3863636363636365	0.10040340909090908	0.7414534090909091	1.4446159090909092	0.2136284090909091	0	0	0	0.03155909090909091	0.03843863636363636	0	In presence of Oxygen
	30	2	16.3758389261745	1.8818791946308726	13.253691275167789	13.344966442953021	5.770469798657717	0	0	0	0.17046979865771814	0	0	In presence of Oxygen
	30	2	14.269005847953219	1.0327485380116959	7.630409356725147	14.869005847953218	2.1988304093567255	0	0	0	0.32397660818713453	0	0	In presence of Oxygen

	31	2	1.1090909090909091	0.10664000000000001	1.6105745454545457	0.08170363636363637	0.45753545454545463	0	0	0.016790909090909092	0	0.024417272727272732	0.013034545454545455	In presence of Oxygen
	31	2	1.0892857142857144	0.1256732142857143	1.5459982142857143	0.10136696428571429	0.42264107142857144	0	0	0.016666071428571433	0	0.027394642857142864	0.017730357142857145	In presence of Oxygen