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Metadata | Values | ||||||||
Asset Title | Maximal specific growth rates of the three lactic acid bacteria and their ldh deletions. | ||||||||
Uploader | Martijn Bekker | ||||||||
Uploader SEEK ID | 53 | ||||||||
Project | SysmoLab | ||||||||
ASSAY | |||||||||
Assay SEEK ID | 107 | ||||||||
Assay Title | Maximal specific growth rates of the three lactic acid bacteria and their ldh deletions | ||||||||
Assay_type | cell_growth_optimisation | ||||||||
Technology_type | chemostat_measurements | ||||||||
Description | |||||||||
Experimentalist | Martijn Bekker | ||||||||
Date | 2008-01-20T0:0:0+0000 | ||||||||
SOP | SOP 2 | ||||||||
Publication (optional) | PMID: 21097579 | ||||||||
Experimental_conditions | |||||||||
Item | temperature | Buffer | Buffer | ||||||
Value | MES buffer | MOPS | |||||||
Unit | ÂșC | mM | mM | ||||||
Start_value (optional) | 37 | 100 | 100 | ||||||
End_value (optional) | |||||||||
Comments | |||||||||
Culture growth | Chemostat | ||||||||
FACTORS_STUDIED | |||||||||
Item | pH | Medium | Medium | ||||||
Value | TH-Broth | CDM-LAB | |||||||
Unit | |||||||||
Start_value (optional) | 6.5 | ||||||||
End_value (optional) | 7.5 | ||||||||
SD (optional) | |||||||||
Comments |
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SAMPLE Name | Treatments (Factors Studied) | Culture_start_date | Date_at_sampling | Age_at_sampling_hours | |||||||||
Organism | NCBI_ID | Strain | Genotype | Phenotype | organism/cell part | pH | Medium | Buffer | date | date | age | Description | |
lacThy00_6.5 | Lactococus Lactis | 1358 | NZ9000 | wild-type | whole cell | 6.5 | TH-Broth | MES Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | ||
lacThy10_6.5 | Lactococus Lactis | 1358 | NZ9010 | wild-type | whole cell | 6.5 | TH-Broth | MES Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | ||
lacThy20_6.5 | Lactococus Lactis | 1358 | NZ9020 | wild-type | 6.5 | TH-Broth | MES Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
efaecThy_6.5 | Enterococcus_faecalis | 1351 | V538 | wild-type | 6.5 | TH-Broth | MES Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
efaecThyLDH_6.5 | Enterococcus_faecalis | 1351 | V538 | ÎLDH | 6.5 | TH-Broth | MES Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
spyThy_6.5 | Streptococcus_pyogenes | 1314 | M49 | wild-type | 6.5 | TH-Broth | MES Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
spyThyLDH_6.5 | Streptococcus_pyogenes | 1314 | M49 | ÎLDH | 6.5 | TH-Broth | MES Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
lacThy00_7.5 | Lactococus Lactis | 1358 | NZ9000 | wild-type | 7.5 | TH-Broth | MOPS Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
lacThy10_7.5 | Lactococus Lactis | 1358 | NZ9010 | ÎLDH | 7.5 | TH-Broth | MOPS Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
lacThy20_7.5 | Lactococus Lactis | 1358 | NZ9020 | ÎLDH,ÎLDHB | 7.5 | TH-Broth | MOPS Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
efaecThy_7.5 | Enterococcus_faecalis | 1351 | V538 | wild-type | 7.5 | TH-Broth | MOPS Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
efaecThyLDH_7.5 | Enterococcus_faecalis | 1351 | V538 | ÎLDH | 7.5 | TH-Broth | MOPS Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
spyThy_7.5 | Streptococcus_pyogenes | 1314 | M49 | wild-type | 7.5 | TH-Broth | MOPS Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
spyThyLDH_7.5 | Streptococcus_pyogenes | 1314 | M49 | ÎLDH | 7.5 | TH-Broth | MOPS Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
lacLAB00_6.5 | Lactococus Lactis | 1358 | NZ9000 | wild-type | 6.5 | CDM-LAB | MES Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
lacLAB10_6.5 | Lactococus Lactis | 1358 | NZ9010 | ÎLDH | 6.5 | CDM-LAB | MES Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
lacLAB20_6.5 | Lactococus Lactis | 1358 | NZ9020 | ÎLDH,ÎLDHB | 6.5 | CDM-LAB | MES Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
efaecLAB_6.5 | Enterococcus_faecalis | 1351 | V538 | wild-type | 6.5 | CDM-LAB | MES Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
efaecLABLDH_6.5 | Enterococcus_faecalis | 1351 | V538 | ÎLDH | 6.5 | CDM-LAB | MES Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
spyLAB_6.5 | Streptococcus_pyogenes | 1314 | M49 | wild-type | 6.5 | CDM-LAB | MES Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
spyLABLDH_6.5 | Streptococcus_pyogenes | 1314 | M49 | ÎLDH | 6.5 | CDM-LAB | MES Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
lacLAB00_7.5 | Lactococus Lactis | 1358 | NZ9000 | wild-type | 7.5 | CDM-LAB | MOPS Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
lacLAB10_7.5 | Lactococus Lactis | 1358 | NZ9010 | ÎLDH | 7.5 | CDM-LAB | MOPS Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
lacLAB20_7.5 | Lactococus Lactis | 1358 | NZ9020 | ÎLDH,ÎLDHB | 7.5 | CDM-LAB | MOPS Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
efaecLAB_7.5 | Enterococcus_faecalis | 1351 | V538 | wild-type | 7.5 | CDM-LAB | MOPS Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
efaecLABLDH_7.5 | Enterococcus_faecalis | 1351 | V538 | ÎLDH | 7.5 | CDM-LAB | MOPS Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
spyLAB_7.5 | Streptococcus_pyogenes | 1314 | M49 | wild-type | 7.5 | CDM-LAB | MOPS Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
spyLABLDH_7.5 | Streptococcus_pyogenes | 1314 | M49 | ÎLDH | 7.5 | CDM-LAB | MOPS Buffer | 2008-01-20T0:0:0+0000 | 2008-01-21T0:0:0+0000 | 24-30 | |||
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Description | Sample | Average growth rate | Standard deviation growth rate | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Unit | h-1 | h-1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
lacThy00_6.5 | 0.7616656405268358 | 0.04733460476558496 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
lacThy10_6.5 | 0.5925951984609136 | 0.045069395066397025 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
lacThy20_6.5 | 0.5267834552736047 | 0.01080787976946251 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
efaecThy_6.5 | 1.1363816559192 | 0.04629692782069011 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
efaecThyLDH_6.5 | 0.9938290430988728 | 0.045933141243477096 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
spyThy_6.5 | 0.8573058224267166 | 0.12611794505686882 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
spyThyLDH_6.5 | 0.6947060298545794 | 0.0027000061796486957 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
lacThy00_7.5 | 0.8126033809120298 | 0.08920987978896305 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
lacThy10_7.5 | 0.7158271818452908 | 0.003617768827372566 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
lacThy20_7.5 | 0.6245106461878255 | 0.011757803268492737 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
efaecThy_7.5 | 1.1551523976729103 | 0.020507201796878382 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
efaecThyLDH_7.5 | 1.014884818641817 | 0.019117245676181692 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
spyThy_7.5 | 0.5715161054776975 | 0.06154978001923539 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
spyThyLDH_7.5 | 0.5320568648118863 | 0.025567169500818564 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
lacLAB00_6.5 | 0.4341181034145592 | 0.0628958825196739 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
lacLAB10_6.5 | 0.453013082636004 | 0.05783184642651589 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
lacLAB20_6.5 | 0.39524834498409084 | 0.04234219929546903 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
efaecLAB_6.5 | 0.741232453232023 | 0.00760034495550198 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
efaecLABLDH_6.5 | 0.6319038544259189 | 0.013675240367050433 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
spyLAB_6.5 | 0.18562373365161408 | 0.029570062384048405 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
spyLABLDH_6.5 | 0.08427998074638439 | 0.02812029541988217 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
lacLAB00_7.5 | 0.49254941416803383 | 0.012094832994393888 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
lacLAB10_7.5 | 0.49129116233349307 | 0.06352355316553986 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
lacLAB20_7.5 | 0.43655490697672183 | 0.02355670691180035 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
efaecLAB_7.5 | 0.7943544825313951 | 0.01346601537569013 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
efaecLABLDH_7.5 | 0.8904150975176465 | 0.07200102496794547 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
spyLAB_7.5 | 0.3580834175696603 | 0.1815410883188106 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
spyLABLDH_7.5 | 0.2586346439375801 | 0.20176806506362935 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||