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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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| Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
| Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
| Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
| Sheets of this workbook | Contents | ||||||||
| Sheet1 | Raw data and sampling plan | ||||||||
| Results-std curve | Data for individual replicates and standard curve | ||||||||
| Repeat for thiamin | |||||||||
| Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
| Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
| Chromatogram | raw data traces | ||||||||
| Credit | |||||||||
| Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
| Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
| File Curation | Andrew Millar | ||||||||
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| numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
| 27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
| 28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
| 28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
| mass tube | Col | prr7,prr9 | ||||||||||||||||
| 27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
| 28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
| 28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
| mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
| 27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
| 28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
| 28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
| mass sample | Col | prr7,prr9 | ||||||||||||||||
| 27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
| 28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
| 28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
| mass sample | Col | prr7,prr9 | ||||||||||||||||
| 27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
| 28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
| 28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
| Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
| 1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
| 2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
| 3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
| 4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
| 5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
| 6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
| 7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
| 8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
| 9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
| 10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
| 11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
| 12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
| 13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
| 14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
| 15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
| 16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
| 17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
| 18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
| 19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
| 20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
| 21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
| 22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
| 23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
| 24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
| 19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
| 7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
| Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
| 25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
| injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
| 20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
| 12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
| 20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
| 8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
| 16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
| 24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
| Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
| Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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| Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
| 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
| 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
| 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
| 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
| 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
| 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
| 7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
| 8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
| 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
| 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
| 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
| 12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
| 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
| 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
| 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
| 16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
| 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
| 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
| 19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
| 20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
| 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
| 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
| 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
| 24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
| Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
| 1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
| 2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
| 3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
| 4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
| 5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
| 6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
| 7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
| 8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
| 9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
| 10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
| 11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
| 12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
| 13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
| 14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
| 15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
| 16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
| 17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
| 18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
| 19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
| 20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
| 21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
| 22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
| 23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
| 24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
| Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
| 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
| 2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
| 3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
| 4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
| 5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
| 6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
| 7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
| 8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
| 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
| 10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
| 11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
| 12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
| 13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
| 14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
| 15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
| 16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
| 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
| 18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
| 19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
| 20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
| 21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
| 22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
| 23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
| 24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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| Sample analysis order | THIA | Extraction volume | Mass | |||||||
| 1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
| 2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
| 3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
| 4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
| 5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
| 6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
| 7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
| 8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
| 9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
| 10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
| 11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
| 12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
| 13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
| 14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
| 15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
| 16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
| 17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
| 18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
| 19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
| 20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
| 21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
| 22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
| 23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
| 24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
| Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
| 1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
| 2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
| 3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
| 4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
| 5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
| 6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
| 7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
| 8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
| 9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
| 10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
| 11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
| 12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
| 13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
| 14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
| 15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
| 16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
| 17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
| 18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
| 19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
| 20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
| 21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
| 22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
| 23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
| 24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
| Sample analysis order | nmol/gFW | THIA | ||||||||
| 1 | Col I | 40.22143360752058 | THIA | |||||||
| 2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
| 3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
| 4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
| 5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
| 6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
| 7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
| 8 | prr7,9 IV | 135.854126984127 | ||||||||
| 9 | Col I | 30.3048327137546 | THIA | |||||||
| 10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
| 11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
| 12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
| 13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
| 14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
| 15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
| 16 | prr7,9 IV | 42.893189401373895 | ||||||||
| 17 | Col I | 34.695187165775366 | ||||||||
| 18 | Col II | 19.566579634464773 | ||||||||
| 19 | Col III | 19.856201005025095 | ||||||||
| 20 | Col IV | 24.15466926070041 | ||||||||
| 21 | prr7,9 I | 48.50650887573963 | ||||||||
| 22 | prr7,9 II | 57.8783116883117 | ||||||||
| 23 | prr7,9 III | 24.452454212454196 | ||||||||
| 24 | prr7,9 IV | 30.054619565217386 |
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| Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
| 27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
| 2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
| 3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
| 4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
| 5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
| 6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
| 7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
| 8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
| 28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
| 10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
| 11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
| 12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
| 13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
| 14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
| 15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
| 16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
| 28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
| 18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
| 19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
| 20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
| 21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
| 22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
| 23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
| 24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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| Sample analysis order | pmol/µL | THIA | |||||||||
| 27 EN | 1 | Col I | 100.55358401880146 | ||||||||
| 2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
| 3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
| 4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
| 5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
| 6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
| 7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
| 8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
| 28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
| 10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
| 11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
| 12 | Col IV | 47.89128508124068 | |||||||||
| 13 | prr7,9 I | 124.83852691218128 | |||||||||
| 14 | prr7,9 II | 65.63786982248523 | |||||||||
| 15 | prr7,9 III | 105.578021978022 | |||||||||
| 16 | prr7,9 IV | 107.23297350343472 | |||||||||
| 28 ED | 17 | Col I | 86.73796791443841 | ||||||||
| 18 | Col II | 48.91644908616194 | |||||||||
| 19 | Col III | 49.640502512562726 | |||||||||
| 20 | Col IV | 60.386673151751026 | |||||||||
| 21 | prr7,9 I | 121.26627218934907 | |||||||||
| 22 | prr7,9 II | 144.6957792207792 | |||||||||
| 23 | prr7,9 III | 61.13113553113548 | |||||||||
| 24 | prr7,9 IV | 75.13654891304348 | |||||||||
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| 150 | 25 | 0 | 75 | |||||||
| 1 pmol | 0.03125 pmol | Col 28 ED | prr79 28 ED | 1 pmol | 0.03125 pmol | Col 28 ED | prr79 28 ED | |||
| 0 | 29.0700614452362 | 29.8625469207764 | 29.0982663631439 | 29.0909588336945 | 0 | 179.0700614452362 | 54.862546920776396 | 29.0982663631439 | 104.0909588336945 | |
| 0.007200046080295 | 29.0654122829437 | 29.8606932163239 | 29.099839925766 | 29.0948450565338 | 0.007200046080295 | 179.0654122829437 | 54.860693216323895 | 29.099839925766 | 104.0948450565338 | |
| 0.01440009216059 | 29.0626466274262 | 29.856824874878 | 29.1010975837708 | 29.0978014469147 | 0.01440009216059 | 179.0626466274262 | 54.856824874878 | 29.1010975837708 | 104.0978014469147 | |
| 0.021600138240885 | 29.0624678134918 | 29.8503398895264 | 29.1018486022949 | 29.098653793335 | 0.021600138240885 | 179.0624678134918 | 54.850339889526396 | 29.1018486022949 | 104.098653793335 | |
| 0.02880018432118 | 29.0654182434082 | 29.8407554626465 | 29.1020452976227 | 29.0966987609863 | 0.02880018432118 | 179.0654182434082 | 54.8407554626465 | 29.1020452976227 | 104.0966987609863 | |
| 0.036000230401475 | 29.0717840194702 | 29.8280656337738 | 29.1017651557923 | 29.0915429592133 | 0.036000230401475 | 179.07178401947021 | 54.828065633773804 | 29.1017651557923 | 104.0915429592133 | |
| 0.04320027648177 | 29.0813684463501 | 29.8129379749298 | 29.1012763977051 | 29.0832281112671 | 0.04320027648177 | 179.0813684463501 | 54.812937974929795 | 29.1012763977051 | 104.0832281112671 | |
| 0.050400322562064 | 29.0933310985565 | 29.7964811325073 | 29.1010797023773 | 29.0725767612457 | 0.050400322562064 | 179.0933310985565 | 54.796481132507296 | 29.1010797023773 | 104.0725767612457 | |
| 0.057600368642359 | 29.1063785552979 | 29.7798037528992 | 29.101836681366 | 29.0613353252411 | 0.057600368642359 | 179.1063785552979 | 54.7798037528992 | 29.101836681366 | 104.0613353252411 | |
| 0.064800414722654 | 29.1190922260285 | 29.7638416290283 | 29.1039526462555 | 29.0516257286072 | 0.064800414722654 | 179.1190922260285 | 54.7638416290283 | 29.1039526462555 | 104.0516257286072 | |
| 0.072000460802949 | 29.1305005550385 | 29.7497153282166 | 29.1071236133576 | 29.0450870990753 | 0.072000460802949 | 179.1305005550385 | 54.749715328216595 | 29.1071236133576 | 104.0450870990753 | |
| 0.079200506883244 | 29.1402459144592 | 29.7389686107636 | 29.1105210781098 | 29.0424406528473 | 0.079200506883244 | 179.1402459144592 | 54.7389686107636 | 29.1105210781098 | 104.0424406528473 | |
| 0.086400552963539 | 29.1483938694 | 29.7332525253296 | 29.1133582592011 | 29.0437042713165 | 0.086400552963539 | 179.1483938694 | 54.733252525329604 | 29.1133582592011 | 104.0437042713165 | |
| 0.093600599043834 | 29.1551053524018 | 29.7335267066956 | 29.1153073310852 | 29.0484488010407 | 0.093600599043834 | 179.1551053524018 | 54.7335267066956 | 29.1153073310852 | 104.0484488010407 | |
| 0.100800645124129 | 29.1602671146393 | 29.739373922348 | 29.1164040565491 | 29.055917263031 | 0.100800645124129 | 179.1602671146393 | 54.739373922348 | 29.1164040565491 | 104.055917263031 | |
| 0.108000691204424 | 29.1635513305664 | 29.7494530677796 | 29.1167259216309 | 29.0649712085724 | 0.108000691204424 | 179.1635513305664 | 54.7494530677796 | 29.1167259216309 | 104.0649712085724 | |
| 0.115200737284719 | 29.1646480560303 | 29.7622799873352 | 29.1162848472595 | 29.0742099285126 | 0.115200737284719 | 179.1646480560303 | 54.762279987335205 | 29.1162848472595 | 104.0742099285126 | |
| 0.122400783365014 | 29.1634142398834 | 29.7766268253327 | 29.1153609752655 | 29.082453250885 | 0.122400783365014 | 179.1634142398834 | 54.7766268253327 | 29.1153609752655 | 104.082453250885 | |
| 0.129600829445309 | 29.1597723960877 | 29.7911167144776 | 29.1146576404572 | 29.0890872478485 | 0.129600829445309 | 179.1597723960877 | 54.791116714477596 | 29.1146576404572 | 104.0890872478485 | |
| 0.136800875525604 | 29.1536509990692 | 29.8038005828858 | 29.1149854660034 | 29.0939688682556 | 0.136800875525604 | 179.1536509990692 | 54.8038005828858 | 29.1149854660034 | 104.0939688682556 | |
| 0.144000921605899 | 29.1450798511505 | 29.8126041889191 | 29.1167914867401 | 29.0971636772156 | 0.144000921605899 | 179.1450798511505 | 54.812604188919096 | 29.1167914867401 | 104.0971636772156 | |
| 0.151200967686193 | 29.1346251964569 | 29.8164367675781 | 29.119884967804 | 29.0988445281983 | 0.151200967686193 | 179.1346251964569 | 54.8164367675781 | 29.119884967804 | 104.0988445281983 | |
| 0.158401013766488 | 29.1237592697144 | 29.8158645629883 | 29.1238367557526 | 29.0994048118592 | 0.158401013766488 | 179.1237592697144 | 54.815864562988295 | 29.1238367557526 | 104.0994048118592 | |
| 0.165601059846783 | 29.1146874427796 | 29.8125207424164 | 29.1281640529633 | 29.0995359420777 | 0.165601059846783 | 179.1146874427796 | 54.812520742416396 | 29.1281640529633 | 104.0995359420777 | |
| 0.172801105927078 | 29.1096270084381 | 29.8080682754517 | 29.1324138641358 | 29.1000545024872 | 0.172801105927078 | 179.1096270084381 | 54.8080682754517 | 29.1324138641358 | 104.1000545024872 | |
| 0.180001152007373 | 29.1101217269898 | 29.8035740852356 | 29.1361033916474 | 29.1014671325684 | 0.180001152007373 | 179.1101217269898 | 54.803574085235596 | 29.1361033916474 | 104.1014671325684 | |
| 0.187201198087668 | 29.1164815425873 | 29.7997176647186 | 29.1387796401978 | 29.1038155555725 | 0.187201198087668 | 179.1164815425873 | 54.7997176647186 | 29.1387796401978 | 104.1038155555725 | |
| 0.194401244167963 | 29.1278243064881 | 29.7971546649933 | 29.1402220726013 | 29.1068196296692 | 0.194401244167963 | 179.1278243064881 | 54.7971546649933 | 29.1402220726013 | 104.1068196296692 | |
| 0.201601290248258 | 29.1423380374909 | 29.7964036464691 | 29.1406273841858 | 29.1102766990662 | 0.201601290248258 | 179.1423380374909 | 54.7964036464691 | 29.1406273841858 | 104.1102766990662 | |
| 0.208801336328553 | 29.1576743125916 | 29.7972917556763 | 29.140442609787 | 29.114180803299 | 0.208801336328553 | 179.1576743125916 | 54.7972917556763 | 29.140442609787 | 104.114180803299 | |
| 0.216001382408848 | 29.1713654994965 | 29.7987520694733 | 29.14018034935 | 29.1186451911926 | 0.216001382408848 | 179.1713654994965 | 54.798752069473295 | 29.14018034935 | 104.1186451911926 | |
| 0.223201428489143 | 29.1812956333161 | 29.799234867096 | 29.1406214237213 | 29.1237533092499 | 0.223201428489143 | 179.1812956333161 | 54.799234867096004 | 29.1406214237213 | 104.1237533092499 | |
| 0.230401474569437 | 29.1860640048981 | 29.7974586486817 | 29.1426777839661 | 29.129558801651 | 0.230401474569437 | 179.1860640048981 | 54.7974586486817 | 29.1426777839661 | 104.129558801651 | |
| 0.237601520649732 | 29.1853904724121 | 29.792982339859 | 29.1470348834992 | 29.1362524032593 | 0.237601520649732 | 179.1853904724121 | 54.792982339859 | 29.1470348834992 | 104.1362524032593 | |
| 0.244801566730027 | 29.1802704334259 | 29.7862946987152 | 29.1536629199982 | 29.1442573070526 | 0.244801566730027 | 179.1802704334259 | 54.786294698715196 | 29.1536629199982 | 104.1442573070526 | |
| 0.252001612810322 | 29.1725873947144 | 29.7784566879273 | 29.1615545749665 | 29.1538834571839 | 0.252001612810322 | 179.1725873947144 | 54.7784566879273 | 29.1615545749665 | 104.1538834571839 | |
| 0.259201658890617 | 29.164457321167 | 29.7706246376038 | 29.1690647602082 | 29.1648805141449 | 0.259201658890617 | 179.164457321167 | 54.7706246376038 | 29.1690647602082 | 104.1648805141449 | |
| 0.266401704970912 | 29.1574716567993 | 29.7638416290283 | 29.1746020317078 | 29.1761815547943 | 0.266401704970912 | 179.1574716567993 | 54.7638416290283 | 29.1746020317078 | 104.1761815547943 | |
| 0.273601751051207 | 29.1523098945618 | 29.7588586807251 | 29.1769087314606 | 29.1862547397614 | 0.273601751051207 | 179.1523098945618 | 54.7588586807251 | 29.1769087314606 | 104.1862547397614 | |
| 0.280801797131502 | 29.1488349437714 | 29.7561466693878 | 29.1751086711884 | 29.1938185691834 | 0.280801797131502 | 179.1488349437714 | 54.7561466693878 | 29.1751086711884 | 104.1938185691834 | |
| 0.288001843211797 | 29.1463971138001 | 29.7557890415192 | 29.1687726974488 | 29.1984140872956 | 0.288001843211797 | 179.1463971138001 | 54.7557890415192 | 29.1687726974488 | 104.1984140872956 | |
| 0.295201889292092 | 29.1440784931183 | 29.7573864459992 | 29.158091545105 | 29.2003273963928 | 0.295201889292092 | 179.1440784931183 | 54.7573864459992 | 29.158091545105 | 104.2003273963928 | |
| 0.302401935372387 | 29.1409194469452 | 29.7601521015167 | 29.1442155838013 | 29.2002260684967 | 0.302401935372387 | 179.1409194469452 | 54.760152101516695 | 29.1442155838013 | 104.2002260684967 | |
| 0.309601981452682 | 29.1364133358002 | 29.7632217407227 | 29.1291773319245 | 29.1988134384155 | 0.309601981452682 | 179.1364133358002 | 54.7632217407227 | 29.1291773319245 | 104.1988134384155 | |
| 0.316802027532976 | 29.1308104991913 | 29.7661602497101 | 29.1153073310852 | 29.1967511177063 | 0.316802027532976 | 179.13081049919128 | 54.7661602497101 | 29.1153073310852 | 104.1967511177063 | |
| 0.324002073613271 | 29.1252136230469 | 29.7692179679871 | 29.104483127594 | 29.1946232318878 | 0.324002073613271 | 179.1252136230469 | 54.7692179679871 | 29.104483127594 | 104.1946232318878 | |
| 0.331202119693566 | 29.1211068630219 | 29.7733426094055 | 29.0977597236634 | 29.1927933692932 | 0.331202119693566 | 179.1211068630219 | 54.7733426094055 | 29.0977597236634 | 104.1927933692932 | |
| 0.338402165773861 | 29.119622707367 | 29.7798275947571 | 29.0952980518341 | 29.1909396648407 | 0.338402165773861 | 179.119622707367 | 54.7798275947571 | 29.0952980518341 | 104.1909396648407 | |
| 0.345602211854156 | 29.1212439537049 | 29.789799451828 | 29.0966153144837 | 29.1880428791046 | 0.345602211854156 | 179.1212439537049 | 54.789799451828 | 29.0966153144837 | 104.1880428791046 | |
| 0.352802257934451 | 29.1258871555329 | 29.8037707805634 | 29.1008472442627 | 29.1828870773316 | 0.352802257934451 | 179.1258871555329 | 54.8037707805634 | 29.1008472442627 | 104.1828870773316 | |
| 0.360002304014746 | 29.1329741477967 | 29.8214614391327 | 29.1068017482758 | 29.1747868061066 | 0.360002304014746 | 179.1329741477967 | 54.821461439132705 | 29.1068017482758 | 104.1747868061066 | |
| 0.367202350095041 | 29.1413962841034 | 29.8418819904328 | 29.1131734848023 | 29.1641354560852 | 0.367202350095041 | 179.1413962841034 | 54.841881990432796 | 29.1131734848023 | 104.1641354560852 | |
| 0.374402396175336 | 29.1494488716126 | 29.8634886741638 | 29.1187644004822 | 29.152262210846 | 0.374402396175336 | 179.1494488716126 | 54.863488674163804 | 29.1187644004822 | 104.152262210846 | |
| 0.381602442255631 | 29.1552603244782 | 29.8842966556549 | 29.1226446628571 | 29.1408658027649 | 0.381602442255631 | 179.1552603244782 | 54.8842966556549 | 29.1226446628571 | 104.14086580276489 | |
| 0.388802488335926 | 29.1575849056244 | 29.9019575119019 | 29.1242599487305 | 29.131543636322 | 0.388802488335926 | 179.1575849056244 | 54.9019575119019 | 29.1242599487305 | 104.131543636322 | |
| 0.39600253441622 | 29.1564643383026 | 29.9140751361847 | 29.1233777999878 | 29.1255652904511 | 0.39600253441622 | 179.1564643383026 | 54.9140751361847 | 29.1233777999878 | 104.1255652904511 | |
| 0.403202580496515 | 29.1529357433319 | 29.918920993805 | 29.1200160980225 | 29.1238367557526 | 0.403202580496515 | 179.1529357433319 | 54.918920993805 | 29.1200160980225 | 104.12383675575259 | |
| 0.41040262657681 | 29.1484355926514 | 29.9160659313202 | 29.1143774986267 | 29.1266679763794 | 0.41040262657681 | 179.1484355926514 | 54.916065931320205 | 29.1143774986267 | 104.1266679763794 | |
| 0.417602672657105 | 29.1441857814789 | 29.9064218997956 | 29.1068494319916 | 29.1336119174957 | 0.417602672657105 | 179.1441857814789 | 54.9064218997956 | 29.1068494319916 | 104.1336119174957 | |
| 0.4248027187374 | 29.1411399841309 | 29.8918187618256 | 29.097980260849 | 29.1433572769165 | 0.4248027187374 | 179.1411399841309 | 54.891818761825604 | 29.097980260849 | 104.1433572769165 | |
| 0.432002764817695 | 29.1401028633118 | 29.8742532730103 | 29.0884613990784 | 29.1541934013367 | 0.432002764817695 | 179.1401028633118 | 54.8742532730103 | 29.0884613990784 | 104.1541934013367 | |
| 0.43920281089799 | 29.1416466236115 | 29.8556625843048 | 29.0793597698212 | 29.1647493839264 | 0.43920281089799 | 179.1416466236115 | 54.855662584304795 | 29.0793597698212 | 104.16474938392639 | |
| 0.446402856978285 | 29.145610332489 | 29.8380374908447 | 29.0719866752625 | 29.1743695735932 | 0.446402856978285 | 179.145610332489 | 54.8380374908447 | 29.0719866752625 | 104.1743695735932 | |
| 0.45360290305858 | 29.1510462760926 | 29.8234701156616 | 29.067611694336 | 29.1830599308014 | 0.45360290305858 | 179.1510462760926 | 54.8234701156616 | 29.067611694336 | 104.18305993080139 | |
| 0.460802949138875 | 29.1565895080567 | 29.8136532306671 | 29.0671050548554 | 29.1910827159882 | 0.460802949138875 | 179.1565895080567 | 54.8136532306671 | 29.0671050548554 | 104.1910827159882 | |
| 0.46800299521917 | 29.1610717773438 | 29.8092484474182 | 29.0706992149353 | 29.1984558105469 | 0.46800299521917 | 179.1610717773438 | 54.8092484474182 | 29.0706992149353 | 104.1984558105469 | |
| 0.475203041299465 | 29.1640043258667 | 29.8096716403961 | 29.0779829025269 | 29.2050302028656 | 0.475203041299465 | 179.1640043258667 | 54.809671640396104 | 29.0779829025269 | 104.2050302028656 | |
| 0.482403087379759 | 29.1653275489807 | 29.8136293888092 | 29.088169336319 | 29.2105853557587 | 0.482403087379759 | 179.1653275489807 | 54.813629388809204 | 29.088169336319 | 104.2105853557587 | |
| 0.489603133460054 | 29.1650950908661 | 29.8198640346527 | 29.1001260280609 | 29.2149722576142 | 0.489603133460054 | 179.1650950908661 | 54.819864034652696 | 29.1001260280609 | 104.21497225761419 | |
| 0.496803179540349 | 29.1634202003479 | 29.8272907733917 | 29.1124224662781 | 29.2179703712464 | 0.496803179540349 | 179.1634202003479 | 54.827290773391695 | 29.1124224662781 | 104.2179703712464 | |
| 0.504003225620644 | 29.1606724262238 | 29.8347234725952 | 29.1236281394959 | 29.2191922664643 | 0.504003225620644 | 179.1606724262238 | 54.8347234725952 | 29.1236281394959 | 104.2191922664643 | |
| 0.511203271700939 | 29.1575610637665 | 29.8406779766083 | 29.1325807571411 | 29.218053817749 | 0.511203271700939 | 179.1575610637665 | 54.840677976608305 | 29.1325807571411 | 104.218053817749 | |
| 0.518403317781234 | 29.1550636291504 | 29.8437535762787 | 29.138731956482 | 29.2142450809479 | 0.518403317781234 | 179.1550636291504 | 54.8437535762787 | 29.138731956482 | 104.2142450809479 | |
| 0.525603363861529 | 29.1539669036865 | 29.8433184623718 | 29.1421353816986 | 29.207992553711 | 0.525603363861529 | 179.1539669036865 | 54.8433184623718 | 29.1421353816986 | 104.207992553711 | |
| 0.532803409941824 | 29.1545867919922 | 29.8397243022919 | 29.1432321071625 | 29.2000532150269 | 0.532803409941824 | 179.1545867919922 | 54.8397243022919 | 29.1432321071625 | 104.2000532150269 | |
| 0.540003456022119 | 29.1569113731384 | 29.8340082168579 | 29.1428625583649 | 29.1913866996765 | 0.540003456022119 | 179.1569113731384 | 54.834008216857896 | 29.1428625583649 | 104.1913866996765 | |
| 0.547203502102414 | 29.1607618331909 | 29.8273921012879 | 29.1421890258789 | 29.1826963424683 | 0.547203502102414 | 179.1607618331909 | 54.8273921012879 | 29.1421890258789 | 104.1826963424683 | |
| 0.554403548182709 | 29.1659116744995 | 29.8211395740509 | 29.1424512863159 | 29.1742742061615 | 0.554403548182709 | 179.1659116744995 | 54.8211395740509 | 29.1424512863159 | 104.1742742061615 | |
| 0.561603594263004 | 29.1718780994415 | 29.8165142536164 | 29.144424200058 | 29.1662871837616 | 0.561603594263004 | 179.1718780994415 | 54.816514253616404 | 29.144424200058 | 104.1662871837616 | |
| 0.568803640343299 | 29.1778802871704 | 29.8146724700928 | 29.1480004787445 | 29.1589260101319 | 0.568803640343299 | 179.1778802871704 | 54.8146724700928 | 29.1480004787445 | 104.15892601013189 | |
| 0.576003686423593 | 29.1829407215119 | 29.8162162303925 | 29.1523039340973 | 29.1525542736054 | 0.576003686423593 | 179.1829407215119 | 54.8162162303925 | 29.1523039340973 | 104.1525542736054 | |
| 0.583203732503888 | 29.1862785816193 | 29.8208713531494 | 29.1562855243683 | 29.1476666927338 | 0.583203732503888 | 179.1862785816193 | 54.8208713531494 | 29.1562855243683 | 104.1476666927338 | |
| 0.590403778584183 | 29.1875898838043 | 29.8275709152222 | 29.1592717170716 | 29.1447281837464 | 0.590403778584183 | 179.1875898838043 | 54.827570915222196 | 29.1592717170716 | 104.1447281837464 | |
| 0.597603824664478 | 29.1870772838593 | 29.8348963260651 | 29.1608035564423 | 29.1441321372986 | 0.597603824664478 | 179.1870772838593 | 54.8348963260651 | 29.1608035564423 | 104.1441321372986 | |
| 0.604803870744773 | 29.1852474212647 | 29.8414826393128 | 29.1604697704315 | 29.1461408138275 | 0.604803870744773 | 179.1852474212647 | 54.8414826393128 | 29.1604697704315 | 104.1461408138275 | |
| 0.612003916825068 | 29.1827321052551 | 29.8463881015778 | 29.1579782962799 | 29.1508495807648 | 0.612003916825068 | 179.1827321052551 | 54.8463881015778 | 29.1579782962799 | 104.1508495807648 | |
| 0.619203962905363 | 29.1800379753113 | 29.84938621521 | 29.1535675525665 | 29.1581630706787 | 0.619203962905363 | 179.1800379753113 | 54.849386215210004 | 29.1535675525665 | 104.1581630706787 | |
| 0.626404008985658 | 29.1773617267609 | 29.8509895801544 | 29.1483283042908 | 29.1678130626679 | 0.626404008985658 | 179.1773617267609 | 54.850989580154405 | 29.1483283042908 | 104.1678130626679 | |
| 0.633604055065953 | 29.1746079921723 | 29.8522412776947 | 29.1438758373261 | 29.179322719574 | 0.633604055065953 | 179.1746079921723 | 54.8522412776947 | 29.1438758373261 | 104.179322719574 | |
| 0.640804101146248 | 29.1715204715729 | 29.8542380332947 | 29.1415572166443 | 29.1918873786926 | 0.640804101146248 | 179.1715204715729 | 54.8542380332947 | 29.1415572166443 | 104.1918873786926 | |
| 0.648004147226543 | 29.1678249835968 | 29.857748746872 | 29.1418433189392 | 29.2043745517731 | 0.648004147226543 | 179.1678249835968 | 54.857748746872005 | 29.1418433189392 | 104.2043745517731 | |
| 0.655204193306837 | 29.1633129119873 | 29.8630475997925 | 29.1444301605225 | 29.2155802249909 | 0.655204193306837 | 179.1633129119873 | 54.863047599792495 | 29.1444301605225 | 104.2155802249909 | |
| 0.662404239387132 | 29.1579484939575 | 29.8699498176575 | 29.1486501693726 | 29.2247295379639 | 0.662404239387132 | 179.1579484939575 | 54.8699498176575 | 29.1486501693726 | 104.2247295379639 | |
| 0.669604285467427 | 29.1520059108734 | 29.877644777298 | 29.1537821292877 | 29.231745004654 | 0.669604285467427 | 179.1520059108734 | 54.877644777298 | 29.1537821292877 | 104.231745004654 | |
| 0.676804331547722 | 29.1462659835816 | 29.8847436904907 | 29.1591227054596 | 29.2369663715363 | 0.676804331547722 | 179.1462659835816 | 54.8847436904907 | 29.1591227054596 | 104.2369663715363 | |
| 0.684004377628017 | 29.1419208049774 | 29.8895657062531 | 29.1637480258942 | 29.2407095432282 | 0.684004377628017 | 179.1419208049774 | 54.8895657062531 | 29.1637480258942 | 104.2407095432282 | |
| 0.691204423708312 | 29.1401445865631 | 29.8907458782196 | 29.1665196418762 | 29.2429447174072 | 0.691204423708312 | 179.1401445865631 | 54.890745878219604 | 29.1665196418762 | 104.2429447174072 | |
| 0.698404469788607 | 29.1417419910431 | 29.8876941204071 | 29.1665494441986 | 29.2436063289643 | 0.698404469788607 | 179.1417419910431 | 54.887694120407104 | 29.1665494441986 | 104.2436063289643 |
