Selected Cell
Cell:
Value:
Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
A
B
C
D
E
F
G
H
I
J
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
A
B
C
D
E
F
G
H
I
J
K
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
A
B
C
D
E
F
G
H
I
J
K
L
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
A
B
C
D
E
F
G
H
I
J
K
801
802
803
804
805
806
807
808
809
810
811
812
813
814
815
816
817
818
819
820
821
822
823
824
825
826
827
828
829
830
831
832
833
834
835
836
837
838
839
840
841
842
843
844
845
846
847
848
849
850
851
852
853
854
855
856
857
858
859
860
861
862
863
864
865
866
867
868
869
870
871
872
873
874
875
876
877
878
879
880
881
882
883
884
885
886
887
888
889
890
891
892
893
894
895
896
897
898
899
900
5.74563677207534 | 29.026073217392 | 29.7260403633118 | 36.6275012493134 | 35.1687490940094 | 5.74563677207534 | 179.026073217392 | 54.726040363311796 | 36.6275012493134 | 110.1687490940094 | |
5.75283681815564 | 29.030579328537 | 29.7423481941223 | 36.519867181778 | 35.0535869598389 | 5.75283681815564 | 179.030579328537 | 54.7423481941223 | 36.519867181778 | 110.0535869598389 | |
5.76003686423593 | 29.0396511554718 | 29.7599375247956 | 36.4041447639466 | 34.9352359771729 | 5.76003686423593 | 179.0396511554718 | 54.7599375247956 | 36.4041447639466 | 109.93523597717291 | |
5.76723691031623 | 29.0529310703278 | 29.7778308391571 | 36.2840652465821 | 34.8179399967194 | 5.76723691031623 | 179.05293107032782 | 54.777830839157104 | 36.2840652465821 | 109.8179399967194 | |
5.77443695639652 | 29.0693461894989 | 29.7951221466065 | 36.1638247966767 | 34.7056984901428 | 5.77443695639652 | 179.0693461894989 | 54.7951221466065 | 36.1638247966767 | 109.7056984901428 | |
5.78163700247682 | 29.0871918201447 | 29.8110365867615 | 36.0475718975067 | 34.6018433570862 | 5.78163700247682 | 179.0871918201447 | 54.8110365867615 | 36.0475718975067 | 109.60184335708621 | |
5.78883704855711 | 29.1043639183045 | 29.8251271247864 | 35.9388768672943 | 34.5086932182312 | 5.78883704855711 | 179.1043639183045 | 54.8251271247864 | 35.9388768672943 | 109.5086932182312 | |
5.79603709463741 | 29.1188418865204 | 29.8373341560364 | 35.840505361557 | 34.4274520874024 | 5.79603709463741 | 179.11884188652039 | 54.8373341560364 | 35.840505361557 | 109.4274520874024 | |
5.8032371407177 | 29.1293442249298 | 29.8480391502381 | 35.7544422149658 | 34.3585371971131 | 5.8032371407177 | 179.1293442249298 | 54.8480391502381 | 35.7544422149658 | 109.3585371971131 | |
5.810437186798 | 29.1356921195984 | 29.8578977584839 | 35.6819450855255 | 34.3019723892212 | 5.810437186798 | 179.1356921195984 | 54.8578977584839 | 35.6819450855255 | 109.30197238922119 | |
5.81763723287829 | 29.13858294487 | 29.8675000667572 | 35.6234788894654 | 34.2576980590821 | 5.81763723287829 | 179.13858294487 | 54.8675000667572 | 35.6234788894654 | 109.2576980590821 | |
5.82483727895859 | 29.1391730308533 | 29.8771381378174 | 35.5784058570862 | 34.2255711555481 | 5.82483727895859 | 179.1391730308533 | 54.8771381378174 | 35.5784058570862 | 109.2255711555481 | |
5.83203732503888 | 29.1385293006897 | 29.8868894577027 | 35.5450451374054 | 34.2051982879639 | 5.83203732503888 | 179.1385293006897 | 54.8868894577027 | 35.5450451374054 | 109.2051982879639 | |
5.83923737111918 | 29.1374564170838 | 29.896628856659 | 35.521012544632 | 34.1958165168762 | 5.83923737111918 | 179.1374564170838 | 54.896628856659 | 35.521012544632 | 109.19581651687619 | |
5.84643741719947 | 29.1364014148712 | 29.9060940742493 | 35.5037152767182 | 34.196412563324 | 5.84643741719947 | 179.13640141487122 | 54.906094074249296 | 35.5037152767182 | 109.196412563324 | |
5.85363746327977 | 29.1354715824127 | 29.9148976802826 | 35.490882396698 | 34.2056930065155 | 5.85363746327977 | 179.1354715824127 | 54.9148976802826 | 35.490882396698 | 109.2056930065155 | |
5.86083750936006 | 29.1344523429871 | 29.9226343631745 | 35.4807138442993 | 34.2219233512879 | 5.86083750936006 | 179.1344523429871 | 54.922634363174495 | 35.4807138442993 | 109.2219233512879 | |
5.86803755544036 | 29.1330099105835 | 29.9292981624603 | 35.4717671871186 | 34.242731332779 | 5.86803755544036 | 179.1330099105835 | 54.9292981624603 | 35.4717671871186 | 109.242731332779 | |
5.87523760152065 | 29.1310369968415 | 29.9355447292328 | 35.462898015976 | 34.2649817466736 | 5.87523760152065 | 179.1310369968415 | 54.9355447292328 | 35.462898015976 | 109.2649817466736 | |
5.88243764760095 | 29.1289210319519 | 29.9424707889557 | 35.453075170517 | 34.2852532863617 | 5.88243764760095 | 179.1289210319519 | 54.9424707889557 | 35.453075170517 | 109.2852532863617 | |
5.88963769368124 | 29.1275560855866 | 29.9510896205902 | 35.44140458107 | 34.30055975914 | 5.88963769368124 | 179.1275560855866 | 54.951089620590196 | 35.44140458107 | 109.30055975914 | |
5.89683773976154 | 29.1279971599579 | 29.9619913101196 | 35.4270875453949 | 34.3088746070862 | 5.89683773976154 | 179.12799715995789 | 54.9619913101196 | 35.4270875453949 | 109.30887460708621 | |
5.90403778584183 | 29.1310608386994 | 29.975301027298 | 35.4093730449677 | 34.3093097209931 | 5.90403778584183 | 179.1310608386994 | 54.975301027298 | 35.4093730449677 | 109.3093097209931 | |
5.91123783192213 | 29.1370511054993 | 29.9908220767975 | 35.3876113891602 | 34.3018174171448 | 5.91123783192213 | 179.1370511054993 | 54.9908220767975 | 35.3876113891602 | 109.3018174171448 | |
5.91843787800242 | 29.1457831859589 | 30.007928609848 | 35.361385345459 | 34.2868387699127 | 5.91843787800242 | 179.1457831859589 | 55.007928609848 | 35.361385345459 | 109.28683876991269 | |
5.92563792408272 | 29.1567325592041 | 30.0254881381989 | 35.3306949138642 | 34.2651426792145 | 5.92563792408272 | 179.1567325592041 | 55.025488138198895 | 35.3306949138642 | 109.2651426792145 | |
5.93283797016301 | 29.1690349578858 | 30.0421357154846 | 35.2961897850037 | 34.2377483844757 | 5.93283797016301 | 179.1690349578858 | 55.042135715484605 | 35.2961897850037 | 109.23774838447571 | |
5.94003801624331 | 29.1815578937531 | 30.0568699836731 | 35.2591753005982 | 34.2058539390564 | 5.94003801624331 | 179.1815578937531 | 55.056869983673096 | 35.2591753005982 | 109.2058539390564 | |
5.9472380623236 | 29.1930437088013 | 30.069500207901 | 35.2214872837067 | 34.1707229614258 | 5.9472380623236 | 179.1930437088013 | 55.069500207901 | 35.2214872837067 | 109.17072296142581 | |
5.9544381084039 | 29.2024850845337 | 30.08052110672 | 35.1851105690003 | 34.133529663086 | 5.9544381084039 | 179.2024850845337 | 55.08052110672 | 35.1851105690003 | 109.133529663086 | |
5.96163815448419 | 29.2094767093659 | 30.0906717777252 | 35.1518154144287 | 34.0954124927521 | 5.96163815448419 | 179.2094767093659 | 55.0906717777252 | 35.1518154144287 | 109.0954124927521 | |
5.96883820056449 | 29.2142450809479 | 30.1005125045777 | 35.1228296756745 | 34.0576887130738 | 5.96883820056449 | 179.2142450809479 | 55.1005125045777 | 35.1228296756745 | 109.0576887130738 | |
5.97603824664478 | 29.2172610759735 | 30.1103293895722 | 35.0988566875458 | 34.0220630168915 | 5.97603824664478 | 179.2172610759735 | 55.1103293895722 | 35.0988566875458 | 109.02206301689151 | |
5.98323829272508 | 29.2189240455628 | 30.1200926303864 | 35.0801408290863 | 33.9906871318817 | 5.98323829272508 | 179.2189240455628 | 55.120092630386395 | 35.0801408290863 | 108.9906871318817 | |
5.99043833880537 | 29.2194068431854 | 30.1295161247254 | 35.0665986537934 | 33.9658498764038 | 5.99043833880537 | 179.2194068431854 | 55.1295161247254 | 35.0665986537934 | 108.96584987640381 | |
5.99763838488567 | 29.2187571525574 | 30.1380217075348 | 35.0576162338257 | 33.9494585990906 | 5.99763838488567 | 179.2187571525574 | 55.138021707534804 | 35.0576162338257 | 108.9494585990906 | |
6.00483843096596 | 29.2169868946076 | 30.1448464393616 | 35.0519716739655 | 33.942449092865 | 6.00483843096596 | 179.2169868946076 | 55.1448464393616 | 35.0519716739655 | 108.94244909286499 | |
6.01203847704626 | 29.2141079902649 | 30.1492035388947 | 35.0479304790497 | 33.9444518089295 | 6.01203847704626 | 179.2141079902649 | 55.149203538894696 | 35.0479304790497 | 108.9444518089295 | |
6.01923852312655 | 29.2100548744202 | 30.1504373550415 | 35.0436449050904 | 33.953607082367 | 6.01923852312655 | 179.2100548744202 | 55.150437355041504 | 35.0436449050904 | 108.953607082367 | |
6.02643856920685 | 29.2047023773194 | 30.1480889320374 | 35.037362575531 | 33.9668035507202 | 6.02643856920685 | 179.2047023773194 | 55.148088932037396 | 35.037362575531 | 108.9668035507202 | |
6.03363861528714 | 29.1980624198914 | 30.1420867443085 | 35.0273132324219 | 33.9800119400025 | 6.03363861528714 | 179.19806241989141 | 55.1420867443085 | 35.0273132324219 | 108.9800119400025 | |
6.04083866136743 | 29.1904509067536 | 30.1327109336853 | 35.0114107131958 | 33.9888215065003 | 6.04083866136743 | 179.1904509067536 | 55.1327109336853 | 35.0114107131958 | 108.9888215065003 | |
6.04803870744773 | 29.1825354099274 | 30.1205217838288 | 34.9872767925263 | 33.9890241622925 | 6.04803870744773 | 179.1825354099274 | 55.1205217838288 | 34.9872767925263 | 108.98902416229251 | |
6.05523875352803 | 29.1751623153687 | 30.1062226295471 | 34.9527359008789 | 33.9771866798401 | 6.05523875352803 | 179.1751623153687 | 55.106222629547105 | 34.9527359008789 | 108.9771866798401 | |
6.06243879960832 | 29.1690230369568 | 30.0905525684357 | 34.9062740802765 | 33.9511156082154 | 6.06243879960832 | 179.1690230369568 | 55.0905525684357 | 34.9062740802765 | 108.9511156082154 | |
6.06963884568862 | 29.1644275188446 | 30.0742864608765 | 34.8469734191895 | 33.9101612567902 | 6.06963884568862 | 179.1644275188446 | 55.0742864608765 | 34.8469734191895 | 108.91016125679019 | |
6.07683889176891 | 29.161274433136 | 30.0583004951477 | 34.7742438316345 | 33.8551938533783 | 6.07683889176891 | 179.161274433136 | 55.058300495147705 | 34.7742438316345 | 108.8551938533783 | |
6.0840389378492 | 29.159277677536 | 30.0435483455658 | 34.6876442432404 | 33.7882697582245 | 6.0840389378492 | 179.159277677536 | 55.043548345565796 | 34.6876442432404 | 108.7882697582245 | |
6.0912389839295 | 29.1580080986023 | 30.030906200409 | 34.5871865749359 | 33.7120890617371 | 6.0912389839295 | 179.1580080986023 | 55.030906200409 | 34.5871865749359 | 108.7120890617371 | |
6.09843903000979 | 29.1568338871002 | 30.0211787223816 | 34.473866224289 | 33.6294531822205 | 6.09843903000979 | 179.1568338871002 | 55.0211787223816 | 34.473866224289 | 108.6294531822205 | |
6.10563907609009 | 29.1548848152161 | 30.0148606300354 | 34.3499064445496 | 33.5427403450012 | 6.10563907609009 | 179.1548848152161 | 55.0148606300354 | 34.3499064445496 | 108.54274034500119 | |
6.11283912217038 | 29.1512131690979 | 30.0118803977967 | 34.2183530330658 | 33.4537148475647 | 6.11283912217038 | 179.1512131690979 | 55.0118803977967 | 34.2183530330658 | 108.4537148475647 | |
6.12003916825068 | 29.1451990604401 | 30.011647939682 | 34.0826451778412 | 33.363550901413 | 6.12003916825068 | 179.1451990604401 | 55.011647939682 | 34.0826451778412 | 108.36355090141299 | |
6.12723921433097 | 29.1369140148163 | 30.0132989883423 | 33.9462697505951 | 33.2730770111084 | 6.12723921433097 | 179.13691401481628 | 55.0132989883423 | 33.9462697505951 | 108.2730770111084 | |
6.13443926041127 | 29.1270852088928 | 30.0160109996796 | 33.8126599788666 | 33.18310379982 | 6.13443926041127 | 179.1270852088928 | 55.0160109996796 | 33.8126599788666 | 108.18310379982 | |
6.14163930649156 | 29.1167438030243 | 30.0190567970276 | 33.6852371692658 | 33.0945789813996 | 6.14163930649156 | 179.1167438030243 | 55.0190567970276 | 33.6852371692658 | 108.0945789813996 | |
6.14883935257186 | 29.1067779064179 | 30.0215303897858 | 33.5669994354248 | 33.0085635185242 | 6.14883935257186 | 179.1067779064179 | 55.021530389785795 | 33.5669994354248 | 108.0085635185242 | |
6.15603939865215 | 29.0979027748108 | 30.0221860408783 | 33.4599614143372 | 32.9260647296906 | 6.15603939865215 | 179.0979027748108 | 55.022186040878296 | 33.4599614143372 | 107.92606472969061 | |
6.16323944473245 | 29.0907144546509 | 30.0198972225189 | 33.3647668361664 | 32.8479886054993 | 6.16323944473245 | 179.0907144546509 | 55.0198972225189 | 33.3647668361664 | 107.8479886054993 | |
6.17043949081274 | 29.0857970714569 | 30.0141394138336 | 33.2807242870331 | 32.7751159667969 | 6.17043949081274 | 179.0857970714569 | 55.014139413833604 | 33.2807242870331 | 107.7751159667969 | |
6.17763953689304 | 29.0834426879883 | 30.0051271915436 | 33.2064211368561 | 32.7079296112061 | 6.17763953689304 | 179.0834426879883 | 55.0051271915436 | 33.2064211368561 | 107.7079296112061 | |
6.18483958297333 | 29.0832579135895 | 29.9934804439545 | 33.1403732299805 | 32.6462686061859 | 6.18483958297333 | 179.0832579135895 | 54.993480443954496 | 33.1403732299805 | 107.6462686061859 | |
6.19203962905363 | 29.0841519832611 | 29.9797773361206 | 33.081328868866 | 32.5891017913819 | 6.19203962905363 | 179.0841519832611 | 54.9797773361206 | 33.081328868866 | 107.58910179138189 | |
6.19923967513392 | 29.0847420692444 | 29.9645304679871 | 33.0281674861908 | 32.534807920456 | 6.19923967513392 | 179.08474206924438 | 54.9645304679871 | 33.0281674861908 | 107.53480792045599 | |
6.20643972121422 | 29.0839612483978 | 29.9485802650452 | 32.9798460006714 | 32.481724023819 | 6.20643972121422 | 179.0839612483978 | 54.9485802650452 | 32.9798460006714 | 107.481724023819 | |
6.21363976729451 | 29.0813028812409 | 29.9331188201905 | 32.9354882240296 | 32.4287414550781 | 6.21363976729451 | 179.0813028812409 | 54.9331188201905 | 32.9354882240296 | 107.4287414550781 | |
6.22083981337481 | 29.0766954421997 | 29.9194693565369 | 32.8948080539704 | 32.3754847049713 | 6.22083981337481 | 179.0766954421997 | 54.9194693565369 | 32.8948080539704 | 107.3754847049713 | |
6.2280398594551 | 29.0703117847443 | 29.9086451530457 | 32.8583180904389 | 32.3222994804383 | 6.2280398594551 | 179.0703117847443 | 54.9086451530457 | 32.8583180904389 | 107.3222994804383 | |
6.2352399055354 | 29.0626287460327 | 29.9011409282685 | 32.8271508216858 | 32.2701334953308 | 6.2352399055354 | 179.06262874603271 | 54.901140928268504 | 32.8271508216858 | 107.27013349533081 | |
6.24243995161569 | 29.0545523166657 | 29.8970699310303 | 32.8026473522186 | 32.220607995987 | 6.24243995161569 | 179.0545523166657 | 54.8970699310303 | 32.8026473522186 | 107.220607995987 | |
6.24963999769599 | 29.047304391861 | 29.8963606357575 | 32.7861547470093 | 32.1757912635803 | 6.24963999769599 | 179.047304391861 | 54.8963606357575 | 32.7861547470093 | 107.1757912635803 | |
6.25684004377628 | 29.0420293807984 | 29.8987329006195 | 32.7790856361389 | 32.1376502513886 | 6.25684004377628 | 179.0420293807984 | 54.8987329006195 | 32.7790856361389 | 107.1376502513886 | |
6.26404008985658 | 29.0394246578217 | 29.9036383628845 | 32.7831327915192 | 32.1074664592743 | 6.26404008985658 | 179.0394246578217 | 54.9036383628845 | 32.7831327915192 | 107.10746645927429 | |
6.27124013593687 | 29.0395677089691 | 29.9102127552033 | 32.8001856803894 | 32.0855617523194 | 6.27124013593687 | 179.0395677089691 | 54.910212755203304 | 32.8001856803894 | 107.08556175231939 | |
6.27844018201717 | 29.0420353412628 | 29.9172401428223 | 32.8319430351258 | 32.0715427398682 | 6.27844018201717 | 179.0420353412628 | 54.9172401428223 | 32.8319430351258 | 107.07154273986819 | |
6.28564022809746 | 29.0459871292114 | 29.9233675003052 | 32.8795075416565 | 32.0644199848175 | 6.28564022809746 | 179.0459871292114 | 54.923367500305204 | 32.8795075416565 | 107.0644199848175 | |
6.29284027417776 | 29.0503442287445 | 29.9274027347565 | 32.9432785511017 | 32.0627331733704 | 6.29284027417776 | 179.0503442287445 | 54.9274027347565 | 32.9432785511017 | 107.06273317337039 | |
6.30004032025805 | 29.0540099143982 | 29.9285411834717 | 33.02321434021 | 32.064425945282 | 6.30004032025805 | 179.0540099143982 | 54.9285411834717 | 33.02321434021 | 107.064425945282 | |
6.30724036633835 | 29.0561139583588 | 29.9264967441559 | 33.119010925293 | 32.0672571659088 | 6.30724036633835 | 179.0561139583588 | 54.9264967441559 | 33.119010925293 | 107.0672571659088 | |
6.31444041241864 | 29.0561199188233 | 29.9215137958527 | 33.2299768924713 | 32.06946849823 | 6.31444041241864 | 179.0561199188233 | 54.921513795852704 | 33.2299768924713 | 107.06946849823001 | |
6.32164045849894 | 29.0538012981415 | 29.9141705036164 | 33.3547949790955 | 32.0703208446503 | 6.32164045849894 | 179.0538012981415 | 54.914170503616404 | 33.3547949790955 | 107.0703208446503 | |
6.32884050457923 | 29.0490865707398 | 29.9052655696869 | 33.491313457489 | 32.0700287818909 | 6.32884050457923 | 179.0490865707398 | 54.905265569686904 | 33.491313457489 | 107.0700287818909 | |
6.33604055065953 | 29.0420949459076 | 29.8956394195557 | 33.6365699768067 | 32.0692479610443 | 6.33604055065953 | 179.0420949459076 | 54.8956394195557 | 33.6365699768067 | 107.0692479610443 | |
6.34324059673982 | 29.0332078933716 | 29.8860490322113 | 33.7870180606842 | 32.0686101913452 | 6.34324059673982 | 179.0332078933716 | 54.886049032211304 | 33.7870180606842 | 107.0686101913452 | |
6.35044064282012 | 29.0230333805084 | 29.876983165741 | 33.9386343955994 | 32.0686519145966 | 6.35044064282012 | 179.0230333805084 | 54.876983165740995 | 33.9386343955994 | 107.0686519145966 | |
6.35764068890041 | 29.0121793746948 | 29.8686742782593 | 34.0870320796967 | 32.0699989795685 | 6.35764068890041 | 179.0121793746948 | 54.8686742782593 | 34.0870320796967 | 107.06999897956851 | |
6.36484073498071 | 29.0011465549469 | 29.8611283302307 | 34.2276990413666 | 32.0733845233917 | 6.36484073498071 | 179.0011465549469 | 54.8611283302307 | 34.2276990413666 | 107.0733845233917 | |
6.372040781061 | 28.9901852607727 | 29.8542082309723 | 34.3564510345459 | 32.0792436599732 | 6.372040781061 | 178.9901852607727 | 54.854208230972304 | 34.3564510345459 | 107.0792436599732 | |
6.3792408271413 | 28.9793848991394 | 29.847663640976 | 34.4699501991272 | 32.0873975753784 | 6.3792408271413 | 178.9793848991394 | 54.847663640975995 | 34.4699501991272 | 107.08739757537839 | |
6.38644087322159 | 28.9687752723694 | 29.8412024974823 | 34.5660865306854 | 32.0969760417938 | 6.38644087322159 | 178.96877527236938 | 54.8412024974823 | 34.5660865306854 | 107.0969760417938 | |
6.39364091930189 | 28.9582848548889 | 29.8346221446991 | 34.6440017223358 | 32.1067154407501 | 6.39364091930189 | 178.9582848548889 | 54.8346221446991 | 34.6440017223358 | 107.1067154407501 | |
6.40084096538218 | 28.9478003978729 | 29.8279225826264 | 34.7038865089417 | 32.1154236793518 | 6.40084096538218 | 178.9478003978729 | 54.8279225826264 | 34.7038865089417 | 107.1154236793518 | |
6.40804101146248 | 28.9371967315674 | 29.8212051391602 | 34.7466826438904 | 32.1222960948944 | 6.40804101146248 | 178.9371967315674 | 54.8212051391602 | 34.7466826438904 | 107.12229609489441 | |
6.41524105754277 | 28.926545381546 | 29.8144638538361 | 34.773987531662 | 32.1268677711487 | 6.41524105754277 | 178.926545381546 | 54.8144638538361 | 34.773987531662 | 107.12686777114871 | |
6.42244110362307 | 28.9162516593933 | 29.8074662685394 | 34.7878873348236 | 32.1288108825684 | 6.42244110362307 | 178.9162516593933 | 54.8074662685394 | 34.7878873348236 | 107.1288108825684 | |
6.42964114970336 | 28.9071559906006 | 29.7999501228333 | 34.7907364368439 | 32.1277618408203 | 6.42964114970336 | 178.9071559906006 | 54.7999501228333 | 34.7907364368439 | 107.1277618408203 | |
6.43684119578366 | 28.9003908634186 | 29.7918260097504 | 34.7846865653992 | 32.1233034133911 | 6.43684119578366 | 178.9003908634186 | 54.7918260097504 | 34.7846865653992 | 107.1233034133911 | |
6.44404124186395 | 28.8970530033112 | 29.7832369804383 | 34.7713649272919 | 32.1153104305268 | 6.44404124186395 | 178.8970530033112 | 54.7832369804383 | 34.7713649272919 | 107.11531043052679 | |
6.45124128794425 | 28.8977682590485 | 29.7744691371918 | 34.7517967224121 | 32.104229927063 | 6.45124128794425 | 178.8977682590485 | 54.7744691371918 | 34.7517967224121 | 107.104229927063 | |
6.45844133402454 | 28.9024233818054 | 29.7657489776612 | 34.7267329692841 | 32.0909976959229 | 6.45844133402454 | 178.9024233818054 | 54.765748977661204 | 34.7267329692841 | 107.09099769592291 |