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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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5.02563216404585 | 28.8811564445496 | 29.7423899173737 | 32.3647081851959 | 31.9038331508637 | 5.02563216404585 | 178.8811564445496 | 54.7423899173737 | 32.3647081851959 | 106.9038331508637 | |
5.03283221012615 | 28.8733303546906 | 29.7387719154358 | 32.3502719402313 | 31.9048225879669 | 5.03283221012615 | 178.8733303546906 | 54.738771915435805 | 32.3502719402313 | 106.9048225879669 | |
5.04003225620644 | 28.8650691509247 | 29.7317802906037 | 32.33482837677 | 31.9038569927216 | 5.04003225620644 | 178.8650691509247 | 54.7317802906037 | 32.33482837677 | 106.9038569927216 | |
5.04723230228674 | 28.8581430912018 | 29.7222435474396 | 32.3193430900574 | 31.9011211395264 | 5.04723230228674 | 178.8581430912018 | 54.722243547439604 | 32.3193430900574 | 106.9011211395264 | |
5.05443234836703 | 28.8542747497559 | 29.7114551067353 | 32.3047578334809 | 31.897646188736 | 5.05443234836703 | 178.8542747497559 | 54.7114551067353 | 32.3047578334809 | 106.897646188736 | |
5.06163239444733 | 28.8547992706299 | 29.7008574008942 | 32.2922348976135 | 31.894999742508 | 5.06163239444733 | 178.8547992706299 | 54.7008574008942 | 32.2922348976135 | 106.89499974250799 | |
5.06883244052762 | 28.8603246212006 | 29.6918690204621 | 32.2831451892853 | 31.8950831890106 | 5.06883244052762 | 178.8603246212006 | 54.6918690204621 | 32.2831451892853 | 106.89508318901059 | |
5.07603248660792 | 28.8703680038452 | 29.6857535839081 | 32.278847694397 | 31.9001138210297 | 5.07603248660792 | 178.87036800384521 | 54.685753583908095 | 32.278847694397 | 106.90011382102969 | |
5.08323253268821 | 28.8833975791931 | 29.6834647655487 | 32.280570268631 | 31.9127321243286 | 5.08323253268821 | 178.88339757919312 | 54.6834647655487 | 32.280570268631 | 106.9127321243286 | |
5.09043257876851 | 28.8972496986389 | 29.6854555606842 | 32.2895050048828 | 31.935715675354 | 5.09043257876851 | 178.8972496986389 | 54.685455560684204 | 32.2895050048828 | 106.935715675354 | |
5.0976326248488 | 28.9099574089051 | 29.6915888786316 | 32.3071360588074 | 31.9713950157166 | 5.0976326248488 | 178.9099574089051 | 54.6915888786316 | 32.3071360588074 | 106.9713950157166 | |
5.1048326709291 | 28.9203882217407 | 29.7011077404022 | 32.3353528976441 | 32.0210039615631 | 5.1048326709291 | 178.9203882217407 | 54.7011077404022 | 32.3353528976441 | 107.02100396156311 | |
5.11203271700939 | 28.9282619953156 | 29.7129333019257 | 32.3760569095612 | 32.0845246315003 | 5.11203271700939 | 178.9282619953156 | 54.7129333019257 | 32.3760569095612 | 107.0845246315003 | |
5.11923276308969 | 28.9338290691376 | 29.7261655330658 | 32.4306011199951 | 32.1608185768128 | 5.11923276308969 | 178.9338290691376 | 54.726165533065796 | 32.4306011199951 | 107.1608185768128 | |
5.12643280916998 | 28.9376795291901 | 29.7403335571289 | 32.4993193149567 | 32.2479009628296 | 5.12643280916998 | 178.9376795291901 | 54.7403335571289 | 32.4993193149567 | 107.24790096282959 | |
5.13363285525028 | 28.9406597614289 | 29.7553360462189 | 32.5813412666321 | 32.3430597782135 | 5.13363285525028 | 178.9406597614289 | 54.7553360462189 | 32.5813412666321 | 107.3430597782135 | |
5.14083290133057 | 28.9437651634217 | 29.7712326049805 | 32.6746344566345 | 32.442831993103 | 5.14083290133057 | 178.9437651634217 | 54.7712326049805 | 32.6746344566345 | 107.442831993103 | |
5.14803294741087 | 28.9478361606598 | 29.7880411148071 | 32.7759861946106 | 32.5433194637299 | 5.14803294741087 | 178.9478361606598 | 54.7880411148071 | 32.7759861946106 | 107.5433194637299 | |
5.15523299349116 | 28.9531111717224 | 29.8056960105896 | 32.881098985672 | 32.6406240463257 | 5.15523299349116 | 178.9531111717224 | 54.8056960105896 | 32.881098985672 | 107.6406240463257 | |
5.16243303957145 | 28.9592266082764 | 29.8241198062897 | 32.9847574234009 | 32.7313423156738 | 5.16243303957145 | 178.9592266082764 | 54.8241198062897 | 32.9847574234009 | 107.7313423156738 | |
5.16963308565175 | 28.9655923843384 | 29.8429310321808 | 33.0815017223358 | 32.8129231929779 | 5.16963308565175 | 178.9655923843384 | 54.8429310321808 | 33.0815017223358 | 107.8129231929779 | |
5.17683313173205 | 28.9719462394715 | 29.8614621162415 | 33.1663310527802 | 32.8836083412171 | 5.17683313173205 | 178.9719462394715 | 54.8614621162415 | 33.1663310527802 | 107.8836083412171 | |
5.18403317781234 | 28.978443145752 | 29.8788905143738 | 33.2353115081787 | 32.942271232605 | 5.18403317781234 | 178.978443145752 | 54.8788905143738 | 33.2353115081787 | 107.94227123260501 | |
5.19123322389264 | 28.9854466915131 | 29.8945963382721 | 33.2859933376312 | 32.9883396625519 | 5.19123322389264 | 178.9854466915131 | 54.894596338272095 | 33.2859933376312 | 107.98833966255191 | |
5.19843326997293 | 28.9932012557984 | 29.908436536789 | 33.3176076412201 | 33.021754026413 | 5.19843326997293 | 178.9932012557984 | 54.908436536789 | 33.3176076412201 | 108.02175402641299 | |
5.20563331605322 | 29.0017306804657 | 29.9207925796509 | 33.3310842514038 | 33.0431163311005 | 5.20563331605322 | 179.0017306804657 | 54.9207925796509 | 33.3310842514038 | 108.04311633110049 | |
5.21283336213352 | 29.0109395980835 | 29.9323081970215 | 33.3289921283722 | 33.0538332462311 | 5.21283336213352 | 179.0109395980835 | 54.9323081970215 | 33.3289921283722 | 108.05383324623111 | |
5.22003340821382 | 29.0205657482147 | 29.943722486496 | 33.3152413368225 | 33.0560743808747 | 5.22003340821382 | 179.0205657482147 | 54.943722486496 | 33.3152413368225 | 108.05607438087469 | |
5.22723345429411 | 29.0300488471985 | 29.9556255340576 | 33.2945764064789 | 33.0524921417237 | 5.22723345429411 | 179.0300488471985 | 54.9556255340576 | 33.2945764064789 | 108.05249214172369 | |
5.23443350037441 | 29.0385961532593 | 29.9681127071381 | 33.2719683647156 | 33.0459475517273 | 5.23443350037441 | 179.0385961532593 | 54.968112707138104 | 33.2719683647156 | 108.0459475517273 | |
5.2416335464547 | 29.0454149246216 | 29.9807071685791 | 33.2520544528961 | 33.0392181873322 | 5.2416335464547 | 179.0454149246216 | 54.9807071685791 | 33.2520544528961 | 108.03921818733221 | |
5.24883359253499 | 29.0500640869141 | 29.9924731254578 | 33.2386195659638 | 33.0348014831543 | 5.24883359253499 | 179.0500640869141 | 54.9924731254578 | 33.2386195659638 | 108.0348014831543 | |
5.25603363861529 | 29.0525674819946 | 30.0024092197418 | 33.234190940857 | 33.0347776412964 | 5.25603363861529 | 179.0525674819946 | 55.0024092197418 | 33.234190940857 | 108.0347776412964 | |
5.26323368469558 | 29.0533602237702 | 30.0097405910492 | 33.239871263504 | 33.0405116081238 | 5.26323368469558 | 179.0533602237702 | 55.0097405910492 | 33.239871263504 | 108.04051160812381 | |
5.27043373077588 | 29.0530979633332 | 30.0140380859375 | 33.2553803920746 | 33.0524027347565 | 5.27043373077588 | 179.0530979633332 | 55.0140380859375 | 33.2553803920746 | 108.0524027347565 | |
5.27763377685618 | 29.0526747703552 | 30.0152003765106 | 33.279424905777 | 33.0699145793915 | 5.27763377685618 | 179.0526747703552 | 55.0152003765106 | 33.279424905777 | 108.06991457939151 | |
5.28483382293647 | 29.0531158447266 | 30.0135314464569 | 33.3100378513336 | 33.0918908119202 | 5.28483382293647 | 179.0531158447266 | 55.013531446456895 | 33.3100378513336 | 108.0918908119202 | |
5.29203386901676 | 29.0552616119385 | 30.0098121166229 | 33.3450436592102 | 33.117014169693 | 5.29203386901676 | 179.0552616119385 | 55.009812116622896 | 33.3450436592102 | 108.117014169693 | |
5.29923391509706 | 29.059499502182 | 30.0051093101502 | 33.3824396133423 | 33.1441938877106 | 5.29923391509706 | 179.059499502182 | 55.0051093101502 | 33.3824396133423 | 108.1441938877106 | |
5.30643396117735 | 29.065614938736 | 30.0001919269562 | 33.4207653999329 | 33.1726729869843 | 5.30643396117735 | 179.065614938736 | 55.000191926956205 | 33.4207653999329 | 108.17267298698431 | |
5.31363400725765 | 29.0729939937592 | 29.9952626228333 | 33.459347486496 | 33.2020401954651 | 5.31363400725765 | 179.0729939937592 | 54.9952626228333 | 33.459347486496 | 108.2020401954651 | |
5.32083405333794 | 29.0809333324433 | 29.9901127815247 | 33.4981203079224 | 33.2323014736176 | 5.32083405333794 | 179.0809333324433 | 54.9901127815247 | 33.4981203079224 | 108.23230147361761 | |
5.32803409941824 | 29.0888488292694 | 29.9844443798065 | 33.5373759269715 | 33.263909816742 | 5.32803409941824 | 179.0888488292694 | 54.984444379806504 | 33.5373759269715 | 108.263909816742 | |
5.33523414549853 | 29.0962636470795 | 29.9781918525696 | 33.577424287796 | 33.2976996898651 | 5.33523414549853 | 179.0962636470795 | 54.9781918525696 | 33.577424287796 | 108.2976996898651 | |
5.34243419157883 | 29.1026890277863 | 29.97145652771 | 33.61856341362 | 33.3347201347351 | 5.34243419157883 | 179.1026890277863 | 54.971456527710004 | 33.61856341362 | 108.33472013473511 | |
5.34963423765912 | 29.107677936554 | 29.9644410610199 | 33.6612164974213 | 33.3759784698487 | 5.34963423765912 | 179.107677936554 | 54.9644410610199 | 33.6612164974213 | 108.37597846984869 | |
5.35683428373942 | 29.1109263896942 | 29.9576163291931 | 33.7058663368225 | 33.422315120697 | 5.35683428373942 | 179.1109263896942 | 54.9576163291931 | 33.7058663368225 | 108.422315120697 | |
5.36403432981971 | 29.1124880313874 | 29.951947927475 | 33.7527990341187 | 33.4744215011597 | 5.36403432981971 | 179.1124880313874 | 54.951947927475004 | 33.7527990341187 | 108.4744215011597 | |
5.37123437590001 | 29.1128754615784 | 29.9486517906189 | 33.8019967079163 | 33.5327386856079 | 5.37123437590001 | 179.1128754615784 | 54.9486517906189 | 33.8019967079163 | 108.53273868560791 | |
5.3784344219803 | 29.1129112243653 | 29.9488008022309 | 33.8531792163849 | 33.5973203182221 | 5.3784344219803 | 179.1129112243653 | 54.9488008022309 | 33.8531792163849 | 108.5973203182221 | |
5.3856344680606 | 29.1134536266327 | 29.9530744552613 | 33.9061498641968 | 33.6676716804505 | 5.3856344680606 | 179.1134536266327 | 54.9530744552613 | 33.9061498641968 | 108.6676716804505 | |
5.39283451414089 | 29.1151583194733 | 29.9616873264313 | 33.9610397815705 | 33.742880821228 | 5.39283451414089 | 179.1151583194733 | 54.9616873264313 | 33.9610397815705 | 108.742880821228 | |
5.40003456022119 | 29.1185855865479 | 29.9745440483093 | 34.0181469917298 | 33.8219165802002 | 5.40003456022119 | 179.1185855865479 | 54.9745440483093 | 34.0181469917298 | 108.8219165802002 | |
5.40723460630148 | 29.1243195533753 | 29.9910604953766 | 34.0777337551117 | 33.9039146900177 | 5.40723460630148 | 179.1243195533753 | 54.9910604953766 | 34.0777337551117 | 108.9039146900177 | |
5.41443465238178 | 29.1328847408295 | 30.0098180770874 | 34.1398775577545 | 33.9881479740143 | 5.41443465238178 | 179.1328847408295 | 55.0098180770874 | 34.1398775577545 | 108.98814797401431 | |
5.42163469846207 | 29.1442513465882 | 30.0287365913391 | 34.2045903205872 | 34.073805809021 | 5.42163469846207 | 179.1442513465882 | 55.0287365913391 | 34.2045903205872 | 109.073805809021 | |
5.42883474454237 | 29.1574358940125 | 30.045634508133 | 34.2720568180084 | 34.1599404811859 | 5.42883474454237 | 179.1574358940125 | 55.045634508133 | 34.2720568180084 | 109.1599404811859 | |
5.43603479062266 | 29.1705489158631 | 30.0589144229889 | 34.3426465988159 | 34.2455625534058 | 5.43603479062266 | 179.1705489158631 | 55.0589144229889 | 34.3426465988159 | 109.24556255340579 | |
5.44323483670296 | 29.1814625263214 | 30.0677001476288 | 34.4165682792664 | 34.3298435211182 | 5.44323483670296 | 179.1814625263214 | 55.0677001476288 | 34.4165682792664 | 109.32984352111819 | |
5.45043488278325 | 29.1884779930115 | 30.0715386867523 | 34.493625164032 | 34.4119787216187 | 5.45043488278325 | 179.1884779930115 | 55.071538686752305 | 34.493625164032 | 109.41197872161871 | |
5.45763492886355 | 29.1904628276825 | 30.0702273845673 | 34.5731198787689 | 34.490978717804 | 5.45763492886355 | 179.1904628276825 | 55.0702273845673 | 34.5731198787689 | 109.490978717804 | |
5.46483497494384 | 29.186886548996 | 30.0640285015106 | 34.6540033817291 | 34.5656037330628 | 5.46483497494384 | 179.186886548996 | 55.0640285015106 | 34.6540033817291 | 109.5656037330628 | |
5.47203502102414 | 29.1777849197388 | 30.0540685653687 | 34.7351908683777 | 34.6345841884613 | 5.47203502102414 | 179.1777849197388 | 55.054068565368695 | 34.7351908683777 | 109.6345841884613 | |
5.47923506710443 | 29.1639804840088 | 30.0421178340912 | 34.8156213760376 | 34.6971094608307 | 5.47923506710443 | 179.1639804840088 | 55.0421178340912 | 34.8156213760376 | 109.6971094608307 | |
5.48643511318473 | 29.1471540927887 | 30.0298273563385 | 34.8942756652832 | 34.7532451152802 | 5.48643511318473 | 179.1471540927887 | 55.0298273563385 | 34.8942756652832 | 109.75324511528021 | |
5.49363515926502 | 29.1293859481812 | 30.018013715744 | 34.9703192710877 | 34.803831577301 | 5.49363515926502 | 179.1293859481812 | 55.018013715744004 | 34.9703192710877 | 109.803831577301 | |
5.50083520534532 | 29.1124701499939 | 30.006742477417 | 35.0435197353363 | 34.8501980304718 | 5.50083520534532 | 179.1124701499939 | 55.006742477417 | 35.0435197353363 | 109.8501980304718 | |
5.50803525142561 | 29.097580909729 | 29.9959421157837 | 35.1143777370453 | 34.8937213420868 | 5.50803525142561 | 179.097580909729 | 54.9959421157837 | 35.1143777370453 | 109.89372134208679 | |
5.51523529750591 | 29.0853023529053 | 29.9858748912811 | 35.1841151714325 | 34.9355399608612 | 5.51523529750591 | 179.0853023529053 | 54.9858748912811 | 35.1841151714325 | 109.9355399608612 | |
5.5224353435862 | 29.0758490562439 | 29.9769103527069 | 35.2541744709015 | 34.9765300750733 | 5.5224353435862 | 179.0758490562439 | 54.976910352706895 | 35.2541744709015 | 109.9765300750733 | |
5.5296353896665 | 29.0691256523133 | 29.9690902233124 | 35.3259801864624 | 35.0173592567444 | 5.5296353896665 | 179.0691256523133 | 54.9690902233124 | 35.3259801864624 | 110.0173592567444 | |
5.53683543574679 | 29.064691066742 | 29.9619436264038 | 35.4008495807648 | 35.0583374500275 | 5.53683543574679 | 179.064691066742 | 54.9619436264038 | 35.4008495807648 | 110.0583374500275 | |
5.54403548182709 | 29.061895608902 | 29.954993724823 | 35.4800283908844 | 35.099333524704 | 5.54403548182709 | 179.061895608902 | 54.954993724823 | 35.4800283908844 | 110.09933352470401 | |
5.55123552790738 | 29.0602028369904 | 29.9481093883515 | 35.5644941329956 | 35.1397752761841 | 5.55123552790738 | 179.0602028369904 | 54.9481093883515 | 35.5644941329956 | 110.13977527618411 | |
5.55843557398768 | 29.0594458580017 | 29.9412548542023 | 35.6547772884369 | 35.1790070533753 | 5.55843557398768 | 179.0594458580017 | 54.9412548542023 | 35.6547772884369 | 110.1790070533753 | |
5.56563562006797 | 29.059648513794 | 29.9338936805725 | 35.750824213028 | 35.2167546749115 | 5.56563562006797 | 179.059648513794 | 54.933893680572496 | 35.750824213028 | 110.2167546749115 | |
5.57283566614827 | 29.0608704090119 | 29.9247860908509 | 35.8522057533264 | 35.2532804012299 | 5.57283566614827 | 179.0608704090119 | 54.9247860908509 | 35.8522057533264 | 110.2532804012299 | |
5.58003571222856 | 29.0629744529724 | 29.9124896526337 | 35.9583616256714 | 35.2891623973847 | 5.58003571222856 | 179.0629744529724 | 54.912489652633695 | 35.9583616256714 | 110.2891623973847 | |
5.58723575830886 | 29.0655672550202 | 29.8961818218231 | 36.068457365036 | 35.3249371051789 | 5.58723575830886 | 179.0655672550202 | 54.8961818218231 | 36.068457365036 | 110.32493710517889 | |
5.59443580438915 | 29.0681064128876 | 29.8759758472443 | 36.1811816692353 | 35.3608131408692 | 5.59443580438915 | 179.0681064128876 | 54.875975847244305 | 36.1811816692353 | 110.3608131408692 | |
5.60163585046945 | 29.0699541568756 | 29.8526227474213 | 36.2945675849915 | 35.3965878486634 | 5.60163585046945 | 179.0699541568756 | 54.8526227474213 | 36.2945675849915 | 110.3965878486634 | |
5.60883589654974 | 29.0704727172852 | 29.8271954059601 | 36.4060878753662 | 35.4318261146546 | 5.60883589654974 | 179.0704727172852 | 54.8271954059601 | 36.4060878753662 | 110.4318261146546 | |
5.61603594263004 | 29.0690779685974 | 29.8011958599091 | 36.5130305290222 | 35.4658722877503 | 5.61603594263004 | 179.0690779685974 | 54.8011958599091 | 36.5130305290222 | 110.4658722877503 | |
5.62323598871033 | 29.0653169155121 | 29.7765791416168 | 36.6127252578736 | 35.4980707168579 | 5.62323598871033 | 179.06531691551208 | 54.7765791416168 | 36.6127252578736 | 110.49807071685791 | |
5.63043603479063 | 29.0589153766632 | 29.7554135322571 | 36.702710390091 | 35.5278909206391 | 5.63043603479063 | 179.0589153766632 | 54.7554135322571 | 36.702710390091 | 110.5278909206391 | |
5.63763608087092 | 29.0500402450562 | 29.739260673523 | 36.7810666561127 | 35.5549573898316 | 5.63763608087092 | 179.0500402450562 | 54.739260673523 | 36.7810666561127 | 110.5549573898316 | |
5.64483612695122 | 29.0395736694336 | 29.7285616397858 | 36.8467032909394 | 35.5790495872498 | 5.64483612695122 | 179.0395736694336 | 54.728561639785795 | 36.8467032909394 | 110.5790495872498 | |
5.65203617303151 | 29.0291249752045 | 29.7225654125214 | 36.8995606899262 | 35.6000900268555 | 5.65203617303151 | 179.0291249752045 | 54.722565412521405 | 36.8995606899262 | 110.6000900268555 | |
5.65923621911181 | 29.0204703807831 | 29.7198355197907 | 36.9405150413513 | 35.6180608272553 | 5.65923621911181 | 179.0204703807831 | 54.7198355197907 | 36.9405150413513 | 110.6180608272553 | |
5.6664362651921 | 29.0149450302124 | 29.7186255455017 | 36.9708716869354 | 35.6327712535858 | 5.6664362651921 | 179.0149450302124 | 54.718625545501695 | 36.9708716869354 | 110.6327712535858 | |
5.67363631127239 | 29.0129542350769 | 29.7172844409943 | 36.9918644428253 | 35.6436014175415 | 5.67363631127239 | 179.0129542350769 | 54.717284440994305 | 36.9918644428253 | 110.6436014175415 | |
5.68083635735269 | 29.014140367508 | 29.71470952034 | 37.004429101944 | 35.6492400169373 | 5.68083635735269 | 179.014140367508 | 54.71470952034 | 37.004429101944 | 110.6492400169373 | |
5.68803640343298 | 29.0177047252655 | 29.7105252742768 | 37.0089888572693 | 35.6476843357086 | 5.68803640343298 | 179.0177047252655 | 54.710525274276804 | 37.0089888572693 | 110.64768433570859 | |
5.69523644951328 | 29.0225982666016 | 29.7051250934601 | 37.0051860809326 | 35.6366157531738 | 5.69523644951328 | 179.0225982666016 | 54.7051250934601 | 37.0051860809326 | 110.6366157531738 | |
5.70243649559358 | 29.0275394916535 | 29.6995460987091 | 36.9918465614319 | 35.6137454509735 | 5.70243649559358 | 179.0275394916535 | 54.6995460987091 | 36.9918465614319 | 110.6137454509735 | |
5.70963654167387 | 29.03111577034 | 29.6951711177826 | 36.9671165943146 | 35.5770349502564 | 5.70963654167387 | 179.03111577034 | 54.6951711177826 | 36.9671165943146 | 110.5770349502564 | |
5.71683658775416 | 29.0322422981262 | 29.6933770179749 | 36.9289517402649 | 35.5250179767609 | 5.71683658775416 | 179.0322422981262 | 54.693377017974896 | 36.9289517402649 | 110.52501797676089 | |
5.72403663383446 | 29.0307998657227 | 29.6953320503235 | 36.875855922699 | 35.4570806026459 | 5.72403663383446 | 179.0307998657227 | 54.6953320503235 | 36.875855922699 | 110.4570806026459 | |
5.73123667991475 | 29.0279269218445 | 29.7016382217407 | 36.8073761463165 | 35.3736996650696 | 5.73123667991475 | 179.0279269218445 | 54.7016382217407 | 36.8073761463165 | 110.37369966506961 | |
5.73843672599505 | 29.0256679058075 | 29.7121107578278 | 36.7240846157074 | 35.2766513824463 | 5.73843672599505 | 179.0256679058075 | 54.7121107578278 | 36.7240846157074 | 110.2766513824463 |