Testing thiamine metabolites in Col and prr7prr9

Selected Cell

Cell:

Value:

Read.Me Sheet1 Results-std curve Repeat for thiamin Graph-stats Graph-stats (2) Chromatogram

Testing thiamine vitamers in plant samples from study L&H3
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites

Analysis Protocol	From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight.

Sheets of this workbook	Contents
Sheet1	Raw data and sampling plan
Results-std curve	Data for individual replicates and standard curve
Repeat for thiamin
Graph-stats	Summary statistics, means, t-test statistics except thiamine, and final charts
Graph-stats (2)	Summary statistics, means, t-test statistics only for thiamine
Chromatogram	raw data traces

Credit
Experimental design, plant growth and sampling	Yin Hoon Chew, Andrew Millar group University of Edinburgh
Analytical design, execution and data analysis	Michael Moulin, Teresa Fitzpatrick group University of Geneva
File Curation	Andrew Millar




numbering	Col				prr7,prr9					replicat	Col				prr7,prr9
27 EN	1	2	3	4	5	6	7	8		27 EN	1	2	3	4	1	2	3	4
28 ED	9	10	11	12	13	14	15	16		28 ED	1	2	3	4	1	2	3	4
28 EN	17	18	19	20	21	22	23	24		28 EN	1	2	3	4	1	2	3	4

mass tube	Col				prr7,prr9
27 EN	0.9438	0.9547	0.9455	0.9373	0.9437	0.9561	0.9486	0.9465
28 ED	0.9427	0.9391	0.9409	0.9434	0.9522	0.9437	0.9438	0.9442
28 EN	0.944	0.9421	0.9439	0.9465	0.9562	0.9436	0.9506	0.9442

mass tube+sample	Col				prr7,prr9
27 EN	1.0289	1.0105	0.992	0.9987	1.0115	1.0188	1.0039	1.0221
28 ED	1.0234	1.036	1.0222	1.0111	1.0581	0.9944	0.9984	1.0461
28 EN	1.0562	1.0187	1.0434	1.0493	1.0576	1.0668	1.0598	1.0178

mass sample	Col				prr7,prr9
27 EN	0.08509999999999995	0.05579999999999996	0.046499999999999986	0.06140000000000001	0.06780000000000008	0.06269999999999998	0.055300000000000016	0.0756
28 ED	0.0807000000000001	0.09689999999999999	0.08130000000000004	0.0677000000000001	0.1059	0.05069999999999997	0.05459999999999998	0.10189999999999999
28 EN	0.11220000000000008	0.07659999999999989	0.09950000000000014	0.10279999999999989	0.10140000000000005	0.12319999999999998	0.10920000000000007	0.0736

mass sample	Col				prr7,prr9
27 EN	300	200	200	200	200	200	200	300
28 ED	300	300	300	200	400	200	200	400
28 EN	300	300	300	300	40	400	400	300


	Extraxtion volume	Mass			Sample analysis order		TDP	TMP	THIA	Extraction volume	Mass
1	300	85.09999999999995			0.03125	pmol	98.93505859375	100.81101989746094	217.923583984375
2	200	55.79999999999996			0.0625	pmol	252.35	237.66	289.42
3	200	46.499999999999986			0.125	pmol	309.66	318.61	576.96
4	200	61.40000000000001			0.25	pmol	502.94	515.39	955.81
5	200	67.80000000000008			0.5	pmol	850.95	859.87	1269.7
6	200	62.699999999999974			1	pmol	1621.6	1673.7	2243.1
7	200	55.30000000000002			2	pmol	3490.9	3645	4747
8	300	75.6			4	pmol	6814.1	7077	9161
9	300	80.7000000000001			1	Col I	2180.39501953125	301.9862060546875	83.60588073730469	300	85.09999999999995
10	300	96.89999999999999			9	Col I	1917.8831787109375	375.14276123046875	132.87686157226562	300	80.7000000000001
11	300	81.30000000000004			17	Col I	2178.0859375	806.1314697265625	72.3534927368164	300	112.20000000000007
12	200	67.70000000000009			5	prr7,9 I	2514.615478515625	299.6667175292969	70.3182601928711	200	67.80000000000008
13	400	105.89999999999999			13	prr7,9 I	1365.0078125	1249.138671875	116.79225158691406	400	105.89999999999999
14	200	50.69999999999997			21	prr7,9 I	1654.855712890625	1042.51416015625	116.10993957519531	400	101.40000000000005
15	200	54.59999999999998			Standard	1	1645.3131103515625	1719.94091796875	2256.296630859375
16	400	101.89999999999999			2	Col II	2157.2509765625	176.75811767578125	54.220611572265625	200	55.79999999999996
17	300	112.20000000000007			10	Col II	1865.593994140625	616.8743286132812	59.84711456298828	300	96.89999999999999
18	200	76.5999999999999			18	Col II	2377.778564453125	465.9914245605469	59.2650146484375	200	76.5999999999999
19	300	99.50000000000014			6	prr7,9 II	2416.207275390625	603.93408203125	58.665550231933594	200	62.699999999999974
20	300	102.7999999999999			14	prr7,9 II	2289.3037109375	526.7593383789062	127.31964874267578	200	50.69999999999997
21	400	101.40000000000005			22	prr7,9 II	1362.8040771484375	1686.056884765625	129.24539184570312	400	123.19999999999997
22	400	123.19999999999997			Standard	0.5	808.2450561523438	831.448486328125	1122.8255615234375
23	400	109.20000000000007			3	Col III	2016.7115478515625	173.78077697753906	65.31547546386719	200	46.499999999999986
24	300	73.6			11	Col III	1981.958740234375	174.57398986816406	21.63164520263672	300	81.30000000000004
					19	Col III	2064.210693359375	473.5988464355469	64.27493286132812	300	99.50000000000014
					7	prr7,9 III	2498.947509765625	391.9966735839844	105.10193634033203	200	55.30000000000002
	Derivatization				15	prr7,9 III	2427.84375	588.3184204101562	63.29181671142578	200	54.59999999999998
	25 µL				23	prr7,9 III	1696.642333984375	1158.29	123.79141235351562	400	109.20000000000007
	injection	injection			Standard	1	1606.5908203125	1679.7308349609375	2153.149169921875
	20 µL				4	Col IV	2237.2646484375	125.17689514160156	43.60393142700195	200	61.40000000000001
					12	Col IV	2196.937255859375	269.585	42.28960418701172	200	67.70000000000009
					20	Col IV	2176.550048828125	417.441	73.04740905761719	300	102.7999999999999
					8	prr7,9 IV	2290.056396484375	659.33	345.0472412109375	300	75.6
					16	prr7,9 IV	1518.505859375	1229.6	95.14126586914062	400	101.89999999999999
					24	prr7,9 IV	2028.2176513671875	424.313	69.16194915771484	300	73.6
					Standard	0.25	447.85986328125	458.4064636230469	788.3421020507812
					Standard	0.03125	98.93505859375	100.81101989746094	217.923583984375



Sample analysis order		TDP	TMP	THIA	Extraction volume	Mass
1	Col I	2180.39501953125	301.9862060546875	83.60588073730469	3.0E-4	0.08509999999999995
2	Col II	2157.2509765625	176.75811767578125	54.220611572265625	1.9999999999999998E-4	0.05579999999999996
3	Col III	2016.7115478515625	173.78077697753906	65.31547546386719	1.9999999999999998E-4	0.046499999999999986
4	Col IV	2237.2646484375	125.17689514160156	43.60393142700195	1.9999999999999998E-4	0.06140000000000002
5	prr7,9 I	2514.615478515625	299.6667175292969	70.3182601928711	1.9999999999999998E-4	0.06780000000000008
6	prr7,9 II	2416.207275390625	603.93408203125	58.665550231933594	1.9999999999999998E-4	0.06269999999999998
7	prr7,9 III	2498.947509765625	391.9966735839844	105.10193634033203	1.9999999999999998E-4	0.05530000000000002				pmol	TDP	TMP	Thia
8	prr7,9 IV	2290.056396484375	659.33	345.0472412109375	3.0E-4	0.0756				3.125E-14	98.93505859375	100.81101989746094	217.923583984375
9	Col I	1917.8831787109375	375.14276123046875	132.87686157226562	3.0E-4	0.0807000000000001				6.25E-14	252.35	237.66	289.42
10	Col II	1865.593994140625	616.8743286132812	59.84711456298828	3.0E-4	0.0969				1.25E-13	309.66	318.61	576.96
11	Col III	1981.958740234375	174.57398986816406	21.63164520263672	3.0E-4	0.08130000000000004				2.5E-13	502.94	515.39	955.81
12	Col IV	2196.937255859375	269.585	42.28960418701172	1.9999999999999998E-4	0.0677000000000001				5.0E-13	850.95	859.87	1269.7
13	prr7,9 I	1365.0078125	1249.138671875	116.79225158691406	3.9999999999999996E-4	0.1059				1.0E-12	1621.6	1673.7	2243.1
14	prr7,9 II	2289.3037109375	526.7593383789062	127.31964874267578	1.9999999999999998E-4	0.05069999999999997				2.0E-12	3490.9	3645	4747
15	prr7,9 III	2427.84375	588.3184204101562	63.29181671142578	1.9999999999999998E-4	0.05459999999999998				4.0E-12	6814.1	7077	9161
16	prr7,9 IV	1518.505859375	1229.6	95.14126586914062	3.9999999999999996E-4	0.10189999999999999				Slope	1.685774878178111E15	1.7555770182597208E15	2.2371156417932975E15
17	Col I	2178.0859375	806.1314697265625	72.3534927368164	3.0E-4	0.11220000000000008				b	63.49	54.786	204.24
18	Col II	2377.778564453125	465.9914245605469	59.2650146484375	1.9999999999999998E-4	0.07659999999999989
19	Col III	2064.210693359375	473.5988464355469	64.27493286132812	3.0E-4	0.09950000000000014
20	Col IV	2176.550048828125	417.441	73.04740905761719	3.0E-4	0.1027999999999999
21	prr7,9 I	1654.855712890625	1042.51416015625	116.10993957519531	3.9999999999999996E-4	0.10140000000000005
22	prr7,9 II	1362.8040771484375	1686.056884765625	129.24539184570312	3.9999999999999996E-4	0.12319999999999998
23	prr7,9 III	1696.642333984375	1158.29	123.79141235351562	3.9999999999999996E-4	0.10920000000000007
24	prr7,9 IV	2028.2176513671875	424.313	69.16194915771484	3.0E-4	0.0736


Sample analysis order	pmol injected	TDP	TMP	THIA			Sample analysis order	pmol/µL	TDP	TMP	THIA
1	Col I	1.2557459758915614E-12	1.4080852248779928E-13	4.180294036865234E-14		27 EN	1	Col I	2.511491951783123E-7	2.8161704497559855E-8	8.360588073730468E-9
2	Col II	1.2420169523615854E-12	6.947693915285502E-14	2.7110305786132812E-14			2	Col II	2.4840339047231705E-7	1.3895387830571003E-8	5.422061157226562E-9
3	Col III	1.1586491014520826E-12	6.778100632434624E-14	3.265773773193359E-14			3	Col III	2.3172982029041653E-7	1.3556201264869248E-8	6.531547546386718E-9
4	Col IV	1.289480983835037E-12	4.009558931876374E-14	2.1801965713500978E-14			4	Col IV	2.5789619676700736E-7	8.019117863752749E-9	4.360393142700195E-9
5	prr7,9 I	1.4540052234997483E-12	1.3948731099934524E-13	3.515913009643555E-14			5	prr7,9 I	2.908010446999496E-7	2.7897462199869047E-8	7.0318260192871094E-9
6	prr7,9 II	1.3956295741773702E-12	3.1280204532160763E-13	2.93327751159668E-14			6	prr7,9 II	2.79125914835474E-7	6.256040906432153E-8	5.8665550231933595E-9
7	prr7,9 III	1.4447109998446106E-12	1.9207968096908483E-13	5.2550968170166016E-14			7	prr7,9 III	2.889421999689221E-7	3.8415936193816965E-8	1.0510193634033203E-8
8	prr7,9 IV	1.3207969968627843E-12	3.443562963698831E-13	1.7252362060546875E-13			8	prr7,9 IV	2.6415939937255685E-7	6.887125927397661E-8	3.450472412109375E-8
9	Col I	1.1000242100624132E-12	1.824794685157328E-13	6.643843078613281E-14		28 EN	9	Col I	2.2000484201248262E-7	3.6495893703146554E-8	1.328768615722656E-8
10	Col II	1.0690063171947585E-12	3.2017298174162225E-13	2.992355728149414E-14			10	Col II	2.138012634389517E-7	6.403459634832445E-8	5.9847114562988275E-9
11	Col III	1.1380337701483285E-12	6.823283092809465E-14	1.081582260131836E-14			11	Col III	2.2760675402966568E-7	1.364656618561893E-8	2.1631645202636718E-9
12	Col IV	1.2655588142143173E-12	1.2235236492952458E-13	2.114480209350586E-14			12	Col IV	2.5311176284286343E-7	2.4470472985904917E-8	4.228960418701171E-9
13	prr7,9 I	7.72059086505433E-13	6.803191540174895E-13	5.839612579345703E-14			13	prr7,9 I	1.5441181730108656E-7	1.360638308034979E-7	1.1679225158691405E-8
14	prr7,9 II	1.3203505045365442E-12	2.6884228573849006E-13	6.365982437133789E-14			14	prr7,9 II	2.640701009073088E-7	5.376845714769801E-8	1.2731964874267577E-8
15	prr7,9 III	1.4025323194727273E-12	3.039071569409353E-13	3.164590835571289E-14			15	prr7,9 III	2.8050646389454546E-7	6.078143138818706E-8	6.329181671142578E-9
16	prr7,9 IV	8.63113976966781E-13	6.691896668621105E-13	4.757063293457031E-14			16	prr7,9 IV	1.726227953933562E-7	1.338379333724221E-7	9.514126586914061E-9
17	Col I	1.254376230701299E-12	4.2797636441570696E-13	3.6176746368408206E-14		28 ED	17	Col I	2.5087524614025976E-7	8.559527288314139E-8	7.235349273681641E-9
18	Col II	1.3728337006387685E-12	2.3422807446418336E-13	2.963250732421875E-14			18	Col II	2.745667401277537E-7	4.684561489283667E-8	5.92650146484375E-9
19	Col III	1.1868255478582284E-12	2.3856136306153646E-13	3.213746643066406E-14			19	Col III	2.3736510957164565E-7	4.771227261230729E-8	6.427493286132813E-9
20	Col IV	1.2534651430514847E-12	2.065731074330734E-13	3.652370452880859E-14			20	Col IV	2.5069302861029693E-7	4.1314621486614676E-8	7.304740905761718E-9
21	prr7,9 I	9.439965759903138E-13	5.626230862462367E-13	5.805496978759765E-14			21	prr7,9 I	1.8879931519806277E-7	1.1252461724924733E-7	1.161099395751953E-8
22	prr7,9 II	7.707518328619666E-13	9.291935744195838E-13	6.462269592285156E-14			22	prr7,9 II	1.541503665723933E-7	1.8583871488391673E-7	1.2924539184570312E-8
23	prr7,9 III	9.687843585315452E-13	6.285705432017378E-13	6.189570617675781E-14			23	prr7,9 III	1.9375687170630903E-7	1.2571410864034754E-7	1.2379141235351562E-8
24	prr7,9 IV	1.16547451074283E-12	2.1048748995718058E-13	3.458097457885742E-14			24	prr7,9 IV	2.3309490214856598E-7	4.209749799143611E-8	6.9161949157714844E-9

Sample analysis order	nmol/gFW	TDP	TMP	THIA
1	Col I	885.3673155522177	99.27745416296075	29.473283456159127			Mean		TDP	TMP	THIA
2	Col II	890.3347328756889	49.80425745724377	19.43391095780131			27 EN	Col	903.1105514312983	58.377214678324826	22.800776756334205
3	Col III	996.6873990985658	58.30624199943764	28.09267761886761			28 ED	Col	766.8519602323545	114.14230855984623	22.100128563367562
4	Col IV	840.0527581987208	26.12090509365715	14.203234992508774			28 EN	Col	708.7355014572627	153.90018407206077	18.87911546319028
5	prr7,9 I	857.8201908553074	82.29339881967259	20.742849614416226			27 EN	prr7,9	960.3561214730375	173.5208146716876	53.59775060445553
6	prr7,9 II	890.3537953284659	199.554733857485	18.71309417286558			28 ED	prr7,9	832.5114124761428	368.51248577692866	38.71740225546809
7	prr7,9 III	1044.9989148966433	138.93647809698714	38.01155021350162			28 EN	prr7,9	726.2764745957589	419.8352113851851	40.32534466004294
8	prr7,9 IV	1048.2515848117334	273.2986479126056	136.92350841703868
9	Col I	817.8618662174064	135.6724672979424	49.39660281496856			STDEV		TDP	TMP	THIA
10	Col II	661.9234162196647	198.24952429821812	18.528518440553643			27 EN	Col	33.180008622075476	15.239203092020402	3.626063867843288
11	Col III	839.8773211426773	50.356332788261696	7.982156901341957			28 ED	Col	40.11419122603355	33.36385488473636	9.351753009264181
12	Col IV	747.7452373496694	72.29090985496272	12.4932360966061			28 EN	Col	13.156048540266871	25.54300793249725	1.2250361762140936
13	prr7,9 I	583.2363259719983	513.9332608252989	44.114164905349966			27 EN	prr7,9	50.252659703550506	40.97956675158741	28.11063756404265
14	prr7,9 II	1041.6966505219289	212.10436744654058	50.22471350795891			28 ED	prr7,9	118.28920963749397	87.31786360652637	5.807422031023975
15	prr7,9 III	1027.4962047419249	222.64260581753507	23.18381564521091			28 EN	prr7,9	92.05967843469897	90.15789119686292	4.134412699440083
16	prr7,9 IV	677.6164686687191	525.36970901834	37.346914963352546
17	Col I	670.7894281825121	228.86436599770408	19.345853672945548			Fold vs Col		TDP	TMP	THIA
18	Col II	716.8844389758591	122.31231042516119	15.473894164082918			27 EN	Col
19	Col III	715.6736972009405	143.85609832856449	19.379376742108956			28 ED	Col
20	Col IV	731.5944414697387	120.56796153681334	21.31733727362371			28 EN	Col
21	prr7,9 I	744.7704741540934	443.88409171300697	45.802737505007975			27 EN	prr7,9	1.0633871124094536	2.9724065395691963	2.3506984510764846
22	prr7,9 II	500.4882031571212	603.3724509218076	41.96278956029323			28 ED	prr7,9	1.0856220700327788	3.2285354171167215	1.7519084626342294
23	prr7,9 III	709.7321307923403	460.4912404408331	45.34483968993242			28 EN	prr7,9	1.0247496747410416	2.7279708202857345	2.135976377636316
24	prr7,9 IV	950.1150902794808	171.59306246509283	28.191011884938113



Sample analysis order		THIA	Extraction volume	Mass
1	Col I	285.237	3.0E-4	0.08509999999999995
2	Col II	121.45	1.9999999999999998E-4	0.05579999999999996
3	Col III	160.685	1.9999999999999998E-4	0.046499999999999986
4	Col IV	150.074	1.9999999999999998E-4	0.06140000000000002
5	prr7,9 I	272.37	1.9999999999999998E-4	0.06780000000000008
6	prr7,9 II	242.808	1.9999999999999998E-4	0.06269999999999998
7	prr7,9 III	164.77	1.9999999999999998E-4	0.05530000000000002				pmol	Thia
8	prr7,9 IV	855.881	3.0E-4	0.0756				6.25E-14	291.3258
9	Col I	203.8	3.0E-4	0.0807000000000001				1.25E-13	464.7634
10	Col II	156.916	3.0E-4	0.0969				2.5E-13	711.93347
11	Col III	135.305	3.0E-4	0.08130000000000004				5.0E-13	964.34528
12	Col IV	162.112	1.9999999999999998E-4	0.0677000000000001				1.0E-12	1763.112
13	prr7,9 I	330.51	3.9999999999999996E-4	0.1059				2.0E-12	4222.5888
14	prr7,9 II	166.392	1.9999999999999998E-4	0.05069999999999997				Slope	1.9906732241935485E15
15	prr7,9 III	288.228	1.9999999999999998E-4	0.05459999999999998				b	256.55
16	prr7,9 IV	273.176	3.9999999999999996E-4	0.10189999999999999
17	Col I	324.4	3.0E-4	0.11220000000000008
18	Col II	187.35	1.9999999999999998E-4	0.07659999999999989
19	Col III	164.641	3.0E-4	0.09950000000000014
20	Col IV	206.925	3.0E-4	0.1027999999999999
21	prr7,9 I	307.41	3.9999999999999996E-4	0.10140000000000005
22	prr7,9 II	445.663	3.9999999999999996E-4	0.12319999999999998
23	prr7,9 III	166.888	3.9999999999999996E-4	0.10920000000000007
24	prr7,9 IV	184.335	3.0E-4	0.0736


Sample analysis order	pmol injected	THIA			Sample analysis order	pmol/µL	THIA
1	Col I	1.4261850000000002E-13		27 EN	1	Col I	1.140948E-8
2	Col II	6.0725E-14			2	Col II	4.8579999999999996E-9
3	Col III	8.03425E-14			3	Col III	6.4274E-9
4	Col IV	7.503700000000001E-14			4	Col IV	6.0029600000000005E-9
5	prr7,9 I	1.36185E-13			5	prr7,9 I	1.0894799999999998E-8
6	prr7,9 II	1.21404E-13			6	prr7,9 II	9.71232E-9
7	prr7,9 III	8.238500000000001E-14			7	prr7,9 III	6.5908E-9
8	prr7,9 IV	4.279405E-13			8	prr7,9 IV	3.423524E-8
9	Col I	1.019E-13		28 EN	9	Col I	8.151999999999999E-9
10	Col II	7.8458E-14			10	Col II	6.276639999999999E-9
11	Col III	6.76525E-14			11	Col III	5.4122E-9
12	Col IV	8.1056E-14			12	Col IV	6.4844799999999995E-9
13	prr7,9 I	1.6525499999999999E-13			13	prr7,9 I	1.3220399999999999E-8
14	prr7,9 II	8.3196E-14			14	prr7,9 II	6.65568E-9
15	prr7,9 III	1.4411400000000002E-13			15	prr7,9 III	1.152912E-8
16	prr7,9 IV	1.3658799999999998E-13			16	prr7,9 IV	1.0927039999999999E-8
17	Col I	1.6219999999999998E-13		28 ED	17	Col I	1.2975999999999998E-8
18	Col II	9.3675E-14			18	Col II	7.493999999999999E-9
19	Col III	8.23205E-14			19	Col III	6.58564E-9
20	Col IV	1.034625E-13			20	Col IV	8.277E-9
21	prr7,9 I	1.5370500000000003E-13			21	prr7,9 I	1.2296400000000002E-8
22	prr7,9 II	2.228315E-13			22	prr7,9 II	1.782652E-8
23	prr7,9 III	8.344400000000001E-14			23	prr7,9 III	6.6755200000000005E-9
24	prr7,9 IV	9.216750000000001E-14			24	prr7,9 IV	7.3734E-9

Sample analysis order	nmol/gFW	THIA
1	Col I	40.22143360752058					THIA
2	Col II	17.412186379928322			27 EN	Col	26.207991701355937
3	Col III	27.644731182795706			28 ED	Col	22.216221609963032
4	Col IV	19.55361563517915			28 EN	Col	24.568159266491413
5	prr7,9 I	32.138053097345086			27 EN	prr7,9	55.70224879793622
6	prr7,9 II	30.980287081339725			28 ED	prr7,9	40.32873922161233
7	prr7,9 III	23.83652802893308			28 EN	prr7,9	40.22297358543073
8	prr7,9 IV	135.854126984127
9	Col I	30.3048327137546					THIA
10	Col II	19.432321981424142			27 EN	Col	5.16468526370469
11	Col III	19.971217712177108			28 ED	Col	2.701505439732336
12	Col IV	19.15651403249628			28 EN	Col	3.534897659186522
13	prr7,9 I	49.935410764872515			27 EN	prr7,9	26.780270173884016
14	prr7,9 II	26.2551479289941			28 ED	prr7,9	5.004580445280961
15	prr7,9 III	42.2312087912088			28 EN	prr7,9	7.812557334225081
16	prr7,9 IV	42.893189401373895
17	Col I	34.695187165775366
18	Col II	19.566579634464773
19	Col III	19.856201005025095
20	Col IV	24.15466926070041
21	prr7,9 I	48.50650887573963
22	prr7,9 II	57.8783116883117
23	prr7,9 III	24.452454212454196
24	prr7,9 IV	30.054619565217386

	Sample analysis order	pmol/µL	TDP	TMP	THIA
27 EN	1	Col I	885.3673155522177	99.27745416296075	29.473283456159127
	2	Col II	890.3347328756889	49.80425745724377	19.43391095780131			TDP	TMP	Thia			TDP	TMP	Thia
	3	Col III	996.6873990985658	58.30624199943764	28.09267761886761	27EN	Col vs prr7.9	0.3784950101359991	0.03887304751915922	0.31894335633099896	27EN	Col vs prr7.9	n.s	*	n.s
	4	Col IV	840.0527581987208	26.12090509365715	14.203234992508774	28ED	Col vs prr7.9	0.6179695046170415	0.034584532576066675	0.18190213222393758	28ED	Col vs prr7.9	n.s	*	n.s
	5	prr7,9 I	857.8201908553074	82.29339881967259	20.742849614416226	28EN	Col vs prr7.9	0.856606669838744	0.029646233508172554	0.002517983320163621	28EN	Col vs prr7.9	n.s	*	**
	6	prr7,9 II	890.3537953284659	199.554733857485	18.71309417286558	Col	27 EN vs 28 EN	0.0397230310306063	0.17923948406921422	0.9465796126527969	Col	27 EN vs 28 EN	*	n.s	n.s
	7	prr7,9 III	1044.9989148966433	138.93647809698714	38.01155021350162	prr7.9	27 EN vs 28 EN	0.3582725251207178	0.08970590900428527	0.6227325689414196	prr7.9	27 EN vs 28 EN	n.s	n.s	n.s
	8	prr7,9 IV	1048.2515848117334	273.2986479126056	136.92350841703868	Col	28 EN vs 28 ED	0.21778917569917583	0.3805817643529822	0.7443616614354294	Col	28 EN vs 28 ED	n.s	n.s	n.s
28 EN	9	Col I	817.8618662174064	135.6724672979424	49.39660281496856	prr7.9	28 EN vs 28 ED	0.5050727905942308	0.6967992204231616	0.8290329831982349	prr7.9	28 EN vs 28 ED	n.s	n.s	n.s
	10	Col II	661.9234162196647	198.24952429821812	18.528518440553643	Col	27 EN vs 28 ED	0.0015937499093712326	0.018331131421305904	0.3450625364371603	Col	27 EN vs 28 ED	**	*	n.s
	11	Col III	839.8773211426773	50.356332788261696	7.982156901341957	prr7.9	27 EN vs 28 ED	0.06709678940629217	0.047343595565421566	0.6568931221269751	prr7.10	27 EN vs 28 ED	n.s	*	n.s
	12	Col IV	747.7452373496694	72.29090985496272	12.4932360966061
	13	prr7,9 I	583.2363259719983	513.9332608252989	44.114164905349966
	14	prr7,9 II	1041.6966505219289	212.10436744654058	50.22471350795891
	15	prr7,9 III	1027.4962047419249	222.64260581753507	23.18381564521091
	16	prr7,9 IV	677.6164686687191	525.36970901834	37.346914963352546
28 ED	17	Col I	670.7894281825121	228.86436599770408	19.345853672945548
	18	Col II	716.8844389758591	122.31231042516119	15.473894164082918
	19	Col III	715.6736972009405	143.85609832856449	19.379376742108956
	20	Col IV	731.5944414697387	120.56796153681334	21.31733727362371
	21	prr7,9 I	744.7704741540934	443.88409171300697	45.802737505007975
	22	prr7,9 II	500.4882031571212	603.3724509218076	41.96278956029323
	23	prr7,9 III	709.7321307923403	460.4912404408331	45.34483968993242
	24	prr7,9 IV	950.1150902794808	171.59306246509283	28.191011884938113

	Sample analysis order	pmol/µL	THIA
27 EN	1	Col I	100.55358401880146
	2	Col II	43.530465949820815			Thia			Thia
	3	Col III	69.11182795698926	27EN	Col vs prr7.9	0.3210491931812907	27EN	Col vs prr7.9	n.s
	4	Col IV	48.884039087947876	28ED	Col vs prr7.9	0.018960465831258648	28ED	Col vs prr7.9	*
	5	prr7,9 I	80.34513274336271	28EN	Col vs prr7.9	0.11769982614260949	28EN	Col vs prr7.9	n.s
	6	prr7,9 II	77.4507177033493	Col	27 EN vs 28 EN	0.5189961799445153	Col	27 EN vs 28 EN	n.s
	7	prr7,9 III	59.5913200723327	prr7.9	27 EN vs 28 EN	0.5930067610455964	prr7.9	27 EN vs 28 EN	n.s
	8	prr7,9 IV	339.6353174603173	Col	28 EN vs 28 ED	0.6160276879003956	Col	28 EN vs 28 ED	n.s
28 EN	9	Col I	75.76208178438651	prr7.9	28 EN vs 28 ED	0.9912742373838004	prr7.9	28 EN vs 28 ED	n.s
	10	Col II	48.58080495356035	Col	27 EN vs 28 ED	0.802073365028804	Col	27 EN vs 28 ED	n.s
	11	Col III	49.92804428044277	prr7.9	27 EN vs 28 ED	0.5990358884307454	prr7.10	27 EN vs 28 ED	n.s
	12	Col IV	47.89128508124068
	13	prr7,9 I	124.83852691218128
	14	prr7,9 II	65.63786982248523
	15	prr7,9 III	105.578021978022
	16	prr7,9 IV	107.23297350343472
28 ED	17	Col I	86.73796791443841
	18	Col II	48.91644908616194
	19	Col III	49.640502512562726
	20	Col IV	60.386673151751026
	21	prr7,9 I	121.26627218934907
	22	prr7,9 II	144.6957792207792
	23	prr7,9 III	61.13113553113548
	24	prr7,9 IV	75.13654891304348

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4.30562755601636	29.4526338577271	30.1046192646027	46.2309122085571	45.2649772167206	4.30562755601636	179.4526338577271	55.104619264602704	46.2309122085571	120.26497721672061
4.31282760209666	29.4230699539185	30.1054894924164	46.5334296226502	45.0608968734741	4.31282760209666	179.4230699539185	55.105489492416396	46.5334296226502	120.06089687347409
4.32002764817695	29.3954968452454	30.1023364067078	46.7453360557556	44.7829842567444	4.32002764817695	179.3954968452454	55.1023364067078	46.7453360557556	119.7829842567444
4.32722769425725	29.3715596199036	30.0962090492249	46.8644320964813	44.4414019584656	4.32722769425725	179.3715596199036	55.096209049224896	46.8644320964813	119.4414019584656
4.33442774033754	29.3518483638764	30.0885498523712	46.8922734260559	44.0473198890686	4.33442774033754	179.3518483638764	55.0885498523712	46.8922734260559	119.0473198890686
4.34162778641784	29.3362319469452	30.0807654857636	46.8338012695313	43.6123847961426	4.34162778641784	179.3362319469452	55.0807654857636	46.8338012695313	118.6123847961426
4.34882783249813	29.3241500854492	30.0739586353302	46.6966331005097	43.1483328342438	4.34882783249813	179.3241500854492	55.0739586353302	46.6966331005097	118.1483328342438
4.35602787857842	29.3148815631867	30.0688326358795	46.4898347854614	42.6666259765625	4.35602787857842	179.3148815631867	55.0688326358795	46.4898347854614	117.6666259765625
4.36322792465872	29.3076932430268	30.0658404827118	46.222847700119	42.1777546405792	4.36322792465872	179.3076932430268	55.0658404827118	46.222847700119	117.1777546405792
4.37042797073902	29.3020129203797	30.0653576850891	45.9048211574555	41.6906118392945	4.37042797073902	179.3020129203797	55.0653576850891	45.9048211574555	116.69061183929449
4.37762801681931	29.2976379394531	30.0675332546234	45.544445514679	41.2124335765839	4.37762801681931	179.2976379394531	55.0675332546234	45.544445514679	116.21243357658389
4.3848280628996	29.2947709560394	30.0721645355225	45.1499164104462	40.7489180564881	4.3848280628996	179.2947709560394	55.072164535522504	45.1499164104462	115.7489180564881
4.3920281089799	29.2936980724335	30.0786793231964	44.7286903858185	40.3042793273926	4.3920281089799	179.2936980724335	55.0786793231964	44.7286903858185	115.3042793273926
4.39922815506019	29.2942941188812	30.0861656665802	44.2874372005463	39.8810923099518	4.39922815506019	179.2942941188812	55.0861656665802	44.2874372005463	114.88109230995181
4.40642820114049	29.2957901954651	30.0936460494995	43.831992149353	39.4802927970886	4.40642820114049	179.2957901954651	55.0936460494995	43.831992149353	114.4802927970886
4.41362824722078	29.2968153953552	30.1002085208893	43.3675229549408	39.1015112400055	4.41362824722078	179.2968153953552	55.100208520889296	43.3675229549408	114.1015112400055
4.42082829330108	29.2958378791809	30.1051676273346	42.8984761238098	38.7437582015991	4.42082829330108	179.2958378791809	55.105167627334595	42.8984761238098	113.74375820159909
4.42802833938137	29.2917609214783	30.1082849502564	42.4285113811493	38.406103849411	4.42802833938137	179.2917609214783	55.108284950256404	42.4285113811493	113.406103849411
4.43522838546167	29.2841374874115	30.1097691059113	41.9606804847717	38.0877852439881	4.43522838546167	179.2841374874115	55.1097691059113	41.9606804847717	113.0877852439881
4.44242843154196	29.2733311653137	30.110228061676	41.4978981018067	37.7882063388825	4.44242843154196	179.2733311653137	55.110228061676	41.4978981018067	112.7882063388825
4.44962847762226	29.2604207992554	30.1104187965393	41.0432755947113	37.5069916248322	4.44962847762226	179.2604207992554	55.1104187965393	41.0432755947113	112.50699162483221
4.45682852370255	29.2469680309296	30.1109910011292	40.6000673770905	37.2440040111542	4.45682852370255	179.2469680309296	55.1109910011292	40.6000673770905	112.2440040111542
4.46402856978285	29.2347967624665	30.1121711730957	40.1714265346527	36.9994282722473	4.46402856978285	179.2347967624665	55.1121711730957	40.1714265346527	111.9994282722473
4.47122861586314	29.2257726192475	30.1136553287506	39.7602498531342	36.7736995220185	4.47122861586314	179.2257726192475	55.113655328750596	39.7602498531342	111.77369952201849
4.47842866194344	29.2213499546051	30.1145613193512	39.3691956996918	36.5673303604126	4.47842866194344	179.2213499546051	55.114561319351196	39.3691956996918	111.5673303604126
4.48562870802373	29.2221903800965	30.1135540008545	39.000678062439	36.380797624588	4.48562870802373	179.2221903800965	55.1135540008545	39.000678062439	111.380797624588
4.49282875410403	29.227751493454	30.1092088222504	38.6567354202271	36.2144529819489	4.49282875410403	179.227751493454	55.1092088222504	38.6567354202271	111.21445298194891
4.50002880018432	29.2363703250885	30.1004409790039	38.338851928711	36.0683977603913	4.50002880018432	179.2363703250885	55.1004409790039	38.338851928711	111.06839776039129
4.50722884626462	29.2457342147827	30.0869524478913	38.0479097366333	35.942417383194	4.50722884626462	179.24573421478271	55.0869524478913	38.0479097366333	110.942417383194
4.51442889234491	29.2536556720734	30.0694346427918	37.7842903137207	35.8359575271607	4.51442889234491	179.2536556720734	55.069434642791805	37.7842903137207	110.8359575271607
4.52162893842521	29.2586028575897	30.049341917038	37.5479698181153	35.7480704784394	4.52162893842521	179.2586028575897	55.049341917038	37.5479698181153	110.7480704784394
4.5288289845055	29.2597472667694	30.0283908843994	37.338650226593	35.6772243976593	4.5288289845055	179.2597472667694	55.0283908843994	37.338650226593	110.6772243976593
4.5360290305858	29.256796836853	30.0082385540009	37.1559023857117	35.6210231781006	4.5360290305858	179.256796836853	55.0082385540009	37.1559023857117	110.6210231781006
4.54322907666609	29.2498409748078	29.9903690814972	36.9991540908814	35.5760335922241	4.54322907666609	179.2498409748078	54.9903690814972	36.9991540908814	110.57603359222409
4.55042912274639	29.2394936084748	29.9761295318604	36.8673801422119	35.5379104614258	4.55042912274639	179.2394936084748	54.9761295318604	36.8673801422119	110.53791046142581
4.55762916882668	29.2269587516785	29.9664556980133	36.7586374282837	35.5015695095062	4.55762916882668	179.2269587516785	54.9664556980133	36.7586374282837	110.5015695095062
4.56482921490698	29.2137980461121	29.9615979194641	36.6697490215302	35.4612648487091	4.56482921490698	179.2137980461121	54.9615979194641	36.6697490215302	110.4612648487091
4.57202926098727	29.2013823986054	29.9610257148743	36.5961730480194	35.4106307029724	4.57202926098727	179.2013823986054	54.961025714874296	36.5961730480194	110.4106307029724
4.57922930706757	29.1904628276825	29.9638748168946	36.5321516990662	35.3428602218628	4.57922930706757	179.1904628276825	54.9638748168946	36.5321516990662	110.3428602218628
4.58642935314786	29.181170463562	29.9694359302521	36.4705324172974	35.251384973526	4.58642935314786	179.181170463562	54.969435930252104	36.4705324172974	110.251384973526
4.59362939922816	29.1734755039215	29.9772143363953	36.402827501297	35.1306736469269	4.59362939922816	179.1734755039215	54.9772143363953	36.402827501297	110.13067364692691
4.60082944530845	29.167515039444	29.9866020679474	36.3197445869446	34.9768400192261	4.60082944530845	179.167515039444	54.9866020679474	36.3197445869446	109.9768400192261
4.60802949138875	29.1635990142822	29.9965739250183	36.2121760845185	34.788066148758	4.60802949138875	179.1635990142822	54.996573925018296	36.2121760845185	109.78806614875799
4.61522953746904	29.1620194911957	30.0058126449585	36.072313785553	34.5650434494019	4.61522953746904	179.1620194911957	55.005812644958496	36.072313785553	109.5650434494019
4.62242958354934	29.1629314422608	30.0131261348725	35.8946621417999	34.3113899230957	4.62242958354934	179.1629314422608	55.0131261348725	35.8946621417999	109.3113899230957
4.62962962962963	29.1664779186249	30.0176322460175	35.6767058372498	34.0339064598084	4.62962962962963	179.1664779186249	55.0176322460175	35.6767058372498	109.0339064598084
4.63682967570993	29.1728794574738	30.018812417984	35.4193866252899	33.7419867515564	4.63682967570993	179.1728794574738	55.018812417984	35.4193866252899	108.7419867515564
4.64402972179022	29.1822910308838	30.0164699554444	35.127329826355	33.4465026855469	4.64402972179022	179.1822910308838	55.0164699554444	35.127329826355	108.4465026855469
4.65122976787052	29.1945815086365	30.010849237442	34.8084688186646	33.1583917140961	4.65122976787052	179.1945815086365	55.010849237442	34.8084688186646	108.1583917140961
4.65842981395081	29.2092263698578	30.0026834011078	34.4731032848358	32.8874886035919	4.65842981395081	179.2092263698578	55.0026834011078	34.4731032848358	107.88748860359189
4.66562986003111	29.2253613471985	29.9932539463043	34.1326415538788	32.6415598392487	4.66562986003111	179.2253613471985	54.9932539463043	34.1326415538788	107.6415598392487
4.6728299061114	29.2420744895935	29.9841463565827	33.798223733902	32.4254810810089	4.6728299061114	179.2420744895935	54.9841463565827	33.798223733902	107.4254810810089
4.6800299521917	29.2585790157318	29.9769818782807	33.4796309471131	32.2407424449921	4.6800299521917	179.2585790157318	54.9769818782807	33.4796309471131	107.2407424449921
4.68722999827199	29.2742311954499	29.9731314182282	33.1844806671143	32.085645198822	4.68722999827199	179.2742311954499	54.9731314182282	33.1844806671143	107.085645198822
4.69443004435229	29.2887806892395	29.9732863903046	32.9178035259247	31.9561958312988	4.69443004435229	179.2887806892395	54.9732863903046	32.9178035259247	106.9561958312988
4.70163009043258	29.3025076389313	29.977285861969	32.6819956302643	31.847494840622	4.70163009043258	179.3025076389313	54.977285861969	32.6819956302643	106.847494840622
4.70883013651288	29.3161749839783	29.9842655658722	32.4770987033844	31.7550122737885	4.70883013651288	179.3161749839783	54.9842655658722	32.4770987033844	106.7550122737885
4.71603018259317	29.3308556079865	29.9931645393372	32.301414012909	31.6754162311554	4.71603018259317	179.3308556079865	54.9931645393372	32.301414012909	106.6754162311554
4.72323022867347	29.3475508689881	30.0029754638672	32.1522414684296	31.6068291664124	4.72323022867347	179.3475508689881	55.0029754638672	32.1522414684296	106.6068291664124
4.73043027475376	29.3668687343598	30.012845993042	32.026731967926	31.5487623214722	4.73043027475376	179.3668687343598	55.012845993042	32.026731967926	106.5487623214722
4.73763032083405	29.3888688087464	30.0219178199768	31.9223761558533	31.5017700195313	4.73763032083405	179.3888688087464	55.0219178199768	31.9223761558533	106.5017700195313
4.74483036691435	29.4129610061646	30.0295472145081	31.8370699882508	31.4667761325836	4.74483036691435	179.4129610061646	55.0295472145081	31.8370699882508	106.4667761325836
4.75203041299465	29.4379234313965	30.0358712673187	31.7689120769501	31.4443469047547	4.75203041299465	179.43792343139648	55.0358712673187	31.7689120769501	106.4443469047547
4.75923045907494	29.4621825218201	30.0419867038727	31.7160546779633	31.4340829849243	4.75923045907494	179.4621825218201	55.041986703872695	31.7160546779633	106.4340829849243
4.76643050515524	29.4839441776276	30.0495207309723	31.6767811775208	31.4343333244324	4.76643050515524	179.4839441776276	55.049520730972304	31.6767811775208	106.4343333244324
4.77363055123553	29.5014917850495	30.0599217414856	31.6495180130005	31.4424932003021	4.77363055123553	179.5014917850495	55.059921741485596	31.6495180130005	106.4424932003021
4.78083059731582	29.5134484767914	30.0740778446198	31.6327154636383	31.4555525779724	4.78083059731582	179.5134484767914	55.0740778446198	31.6327154636383	106.4555525779724
4.78803064339612	29.5190274715424	30.092465877533	31.624585390091	31.4706444740296	4.78803064339612	179.51902747154242	55.092465877533	31.624585390091	106.4706444740296
4.79523068947642	29.5181095600128	30.1153957843781	31.6230416297913	31.4854264259339	4.79523068947642	179.5181095600128	55.1153957843781	31.6230416297913	106.4854264259339
4.80243073555671	29.5112192630768	30.1427841186524	31.6261470317841	31.4984679222107	4.80243073555671	179.5112192630768	55.1427841186524	31.6261470317841	106.4984679222107
4.809630781637	29.4991374015808	30.1737010478974	31.6325604915619	31.5093338489533	4.809630781637	179.4991374015808	55.173701047897396	31.6325604915619	106.5093338489533
4.8168308277173	29.4826626777649	30.2063286304474	31.641685962677	31.5185010433197	4.8168308277173	179.4826626777649	55.2063286304474	31.641685962677	106.5185010433197
4.82403087379759	29.4623851776123	30.2383661270142	31.6534161567688	31.5271019935608	4.82403087379759	179.4623851776123	55.2383661270142	31.6534161567688	106.5271019935608
4.83123091987789	29.4386744499207	30.2675247192383	31.6677629947663	31.5363824367523	4.83123091987789	179.4386744499207	55.267524719238295	31.6677629947663	106.5363824367523
4.83843096595818	29.4116973876953	30.2917301654816	31.6846609115601	31.5472900867462	4.83843096595818	179.4116973876953	55.291730165481596	31.6846609115601	106.5472900867462
4.84563101203848	29.381388425827	30.3091287612915	31.7040503025055	31.5603792667389	4.84563101203848	179.381388425827	55.309128761291504	31.7040503025055	106.56037926673889
4.85283105811877	29.3475508689881	30.3181648254395	31.7258000373841	31.5757632255554	4.85283105811877	179.3475508689881	55.318164825439496	31.7258000373841	106.57576322555539
4.86003110419907	29.3100833892822	30.3177773952484	31.7496478557587	31.59299492836	4.86003110419907	179.3100833892822	55.3177773952484	31.7496478557587	106.59299492836
4.86723115027936	29.2692482471466	30.3074955940247	31.775176525116	31.6112399101258	4.86723115027936	179.2692482471466	55.3074955940247	31.775176525116	106.6112399101258
4.87443119635966	29.225903749466	30.2873730659485	31.8019986152649	31.6294968128205	4.87443119635966	179.225903749466	55.2873730659485	31.8019986152649	106.62949681282049
4.88163124243995	29.1814267635346	30.2578151226044	31.829982995987	31.6469788551331	4.88163124243995	179.1814267635346	55.2578151226044	31.829982995987	106.6469788551331
4.88883128852025	29.1375875473023	30.2195250988007	31.8593859672547	31.6635549068451	4.88883128852025	179.1375875473023	55.2195250988007	31.8593859672547	106.66355490684509
4.89603133460054	29.0962159633637	30.1735043525696	31.8907976150513	31.6796362400055	4.89603133460054	179.0962159633637	55.1735043525696	31.8907976150513	106.6796362400055
4.90323138068084	29.0589272975922	30.1211416721344	31.9249868392945	31.6959083080292	4.90323138068084	179.0589272975922	55.1211416721344	31.9249868392945	106.6959083080292
4.91043142676113	29.0268063545227	30.0643086433411	31.9627344608307	31.7131102085114	4.91043142676113	179.0268063545227	55.0643086433411	31.9627344608307	106.7131102085114
4.91763147284143	29.0002107620239	30.0052881240845	32.0045053958893	31.7318975925446	4.91763147284143	179.0002107620239	55.0052881240845	32.0045053958893	106.7318975925446
4.92483151892172	28.9787769317627	29.9466907978058	32.0500671863556	31.7525804042816	4.92483151892172	178.9787769317627	54.9466907978058	32.0500671863556	106.7525804042816
4.93203156500202	28.9615273475647	29.8911273479462	32.0984184741974	31.7749738693238	4.93203156500202	178.9615273475647	54.891127347946195	32.0984184741974	106.7749738693238
4.93923161108231	28.9472699165344	29.8409044742584	32.1479320526123	31.7981660366059	4.93923161108231	178.9472699165344	54.8409044742584	32.1479320526123	106.79816603660589
4.94643165716261	28.9349615573883	29.7979235649109	32.1966946125031	31.820696592331	4.94643165716261	178.9349615573883	54.7979235649109	32.1966946125031	106.820696592331
4.9536317032429	28.9239466190338	29.7636270523071	32.2427749633789	31.8410038948059	4.9536317032429	178.9239466190338	54.7636270523071	32.2427749633789	106.84100389480591
4.9608317493232	28.9140403270722	29.7388136386872	32.284277677536	31.8579137325287	4.9608317493232	178.9140403270722	54.738813638687205	32.284277677536	106.8579137325287
4.96803179540349	28.9054811000824	29.7234177589417	32.3196113109589	31.8709075450897	4.96803179540349	178.9054811000824	54.7234177589417	32.3196113109589	106.8709075450897
4.97523184148379	28.8987338542938	29.7163307666779	32.3477506637573	31.8801522254944	4.97523184148379	178.8987338542938	54.7163307666779	32.3477506637573	106.8801522254944
4.98243188756408	28.8942277431488	29.7157466411591	32.3684215545655	31.8863272666931	4.98243188756408	178.8942277431488	54.7157466411591	32.3684215545655	106.8863272666931
4.98963193364438	28.8920044898987	29.7195911407471	32.3819696903229	31.8903684616089	4.98963193364438	178.8920044898987	54.7195911407471	32.3819696903229	106.8903684616089
4.99683197972467	28.8914978504181	29.7259032726288	32.3890089988709	31.8932592868805	4.99683197972467	178.8914978504181	54.7259032726288	32.3890089988709	106.8932592868805
5.00403202580497	28.8914680480957	29.7328591346741	32.3900938034058	31.8958282470703	5.00403202580497	178.8914680480957	54.7328591346741	32.3900938034058	106.8958282470703
5.01123207188526	28.8903951644898	29.7387957572937	32.3858082294464	31.8985879421234	5.01123207188526	178.8903951644898	54.7387957572937	32.3858082294464	106.8985879421234
5.01843211796556	28.8870692253113	29.742294549942	32.3769748210907	31.9014608860016	5.01843211796556	178.8870692253113	54.742294549942	32.3769748210907	106.9014608860016