Selected Cell
Cell:
Value:
Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
A
B
C
D
E
F
G
H
I
J
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
A
B
C
D
E
F
G
H
I
J
K
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
A
B
C
D
E
F
G
H
I
J
K
L
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
A
B
C
D
E
F
G
H
I
J
K
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
2.86561833995738 | 29.2059004306793 | 29.8789441585541 | 33.3583116531372 | 32.6422035694123 | 2.86561833995738 | 179.2059004306793 | 54.8789441585541 | 33.3583116531372 | 107.6422035694123 | |
2.87281838603767 | 29.1995108127594 | 29.8609137535095 | 33.2566142082215 | 32.5788855552674 | 2.87281838603767 | 179.1995108127594 | 54.8609137535095 | 33.2566142082215 | 107.57888555526739 | |
2.88001843211797 | 29.1953504085541 | 29.8433721065521 | 33.1600248813629 | 32.5213968753815 | 2.88001843211797 | 179.1953504085541 | 54.843372106552096 | 33.1600248813629 | 107.5213968753815 | |
2.88721847819826 | 29.1935324668884 | 29.8290610313416 | 33.0694317817688 | 32.4687838554383 | 2.88721847819826 | 179.1935324668884 | 54.829061031341595 | 33.0694317817688 | 107.4687838554383 | |
2.89441852427856 | 29.193788766861 | 29.8202931880951 | 32.9855084419251 | 32.4197471141815 | 2.89441852427856 | 179.193788766861 | 54.8202931880951 | 32.9855084419251 | 107.41974711418149 | |
2.90161857035885 | 29.1953206062317 | 29.8184871673584 | 32.908445596695 | 32.372796535492 | 2.90161857035885 | 179.1953206062317 | 54.8184871673584 | 32.908445596695 | 107.372796535492 | |
2.90881861643915 | 29.1969537734986 | 29.8239529132843 | 32.8378260135651 | 32.3266625404358 | 2.90881861643915 | 179.1969537734986 | 54.8239529132843 | 32.8378260135651 | 107.32666254043579 | |
2.91601866251944 | 29.197633266449 | 29.8357367515564 | 32.7726483345032 | 32.2804689407349 | 2.91601866251944 | 179.197633266449 | 54.8357367515564 | 32.7726483345032 | 107.28046894073489 | |
2.92321870859974 | 29.1967391967774 | 29.851633310318 | 32.7112972736359 | 32.2340071201325 | 2.92321870859974 | 179.1967391967774 | 54.851633310318 | 32.7112972736359 | 107.2340071201325 | |
2.93041875468003 | 29.1941046714783 | 29.8685491085053 | 32.6517343521118 | 32.1876227855683 | 2.93041875468003 | 179.1941046714783 | 54.8685491085053 | 32.6517343521118 | 107.1876227855683 | |
2.93761880076033 | 29.1897535324097 | 29.8832833766937 | 32.5920104980469 | 32.1417272090912 | 2.93761880076033 | 179.1897535324097 | 54.8832833766937 | 32.5920104980469 | 107.1417272090912 | |
2.94481884684062 | 29.1838586330414 | 29.8934757709503 | 32.5307607650757 | 32.0965826511383 | 2.94481884684062 | 179.1838586330414 | 54.8934757709503 | 32.5307607650757 | 107.0965826511383 | |
2.95201889292092 | 29.1768074035645 | 29.8980951309204 | 32.4674665927887 | 32.0521950721741 | 2.95201889292092 | 179.1768074035645 | 54.898095130920396 | 32.4674665927887 | 107.0521950721741 | |
2.95921893900121 | 29.1692852973938 | 29.8975944519043 | 32.4022829532624 | 32.0086002349854 | 2.95921893900121 | 179.1692852973938 | 54.8975944519043 | 32.4022829532624 | 107.00860023498541 | |
2.96641898508151 | 29.1619598865509 | 29.8935651779175 | 32.3356926441193 | 31.9661259651184 | 2.96641898508151 | 179.1619598865509 | 54.893565177917495 | 32.3356926441193 | 106.96612596511841 | |
2.9736190311618 | 29.1551947593689 | 29.8883020877838 | 32.2684526443482 | 31.9253265857697 | 2.9736190311618 | 179.1551947593689 | 54.8883020877838 | 32.2684526443482 | 106.9253265857697 | |
2.9808190772421 | 29.1489124298096 | 29.8843383789063 | 32.2018086910248 | 31.8865418434143 | 2.9808190772421 | 179.1489124298096 | 54.8843383789063 | 32.2018086910248 | 106.8865418434143 | |
2.98801912332239 | 29.1428446769715 | 29.8838078975678 | 32.1374177932739 | 31.8498015403748 | 2.98801912332239 | 179.1428446769715 | 54.8838078975678 | 32.1374177932739 | 106.8498015403748 | |
2.99521916940269 | 29.1369020938874 | 29.8877596855164 | 32.0771396160126 | 31.814980506897 | 2.99521916940269 | 179.1369020938874 | 54.8877596855164 | 32.0771396160126 | 106.814980506897 | |
3.00241921548298 | 29.1313529014588 | 29.895830154419 | 32.0225238800049 | 31.7821323871613 | 3.00241921548298 | 179.1313529014588 | 54.895830154419 | 32.0225238800049 | 106.7821323871613 | |
3.00961926156328 | 29.1265666484833 | 29.9062609672547 | 31.9745838642121 | 31.751811504364 | 3.00961926156328 | 179.1265666484833 | 54.906260967254696 | 31.9745838642121 | 106.751811504364 | |
3.01681930764357 | 29.1226267814636 | 29.9164533615113 | 31.9339036941529 | 31.724888086319 | 3.01681930764357 | 179.1226267814636 | 54.9164533615113 | 31.9339036941529 | 106.724888086319 | |
3.02401935372387 | 29.1192412376404 | 29.9236297607422 | 31.9007396697998 | 31.7020833492279 | 3.02401935372387 | 179.1192412376404 | 54.9236297607422 | 31.9007396697998 | 106.7020833492279 | |
3.03121939980416 | 29.1159212589264 | 29.9253463745117 | 31.8749845027924 | 31.6837549209595 | 3.03121939980416 | 179.1159212589264 | 54.925346374511705 | 31.8749845027924 | 106.6837549209595 | |
3.03841944588446 | 29.1124224662781 | 29.9198806285858 | 31.8559288978577 | 31.6701054573059 | 3.03841944588446 | 179.1124224662781 | 54.9198806285858 | 31.8559288978577 | 106.67010545730591 | |
3.04561949196475 | 29.1089057922363 | 29.906690120697 | 31.8422377109528 | 31.6616177558899 | 3.04561949196475 | 179.1089057922363 | 54.906690120697 | 31.8422377109528 | 106.66161775588989 | |
3.05281953804505 | 29.1056036949158 | 29.8867821693421 | 31.8322122097016 | 31.6594362258911 | 3.05281953804505 | 179.1056036949158 | 54.8867821693421 | 31.8322122097016 | 106.6594362258911 | |
3.06001958412534 | 29.1025698184967 | 29.8624992370606 | 31.8245589733124 | 31.665176153183 | 3.06001958412534 | 179.1025698184967 | 54.862499237060604 | 31.8245589733124 | 106.665176153183 | |
3.06721963020564 | 29.0996015071869 | 29.8370599746704 | 31.8190574645996 | 31.6803514957428 | 3.06721963020564 | 179.0996015071869 | 54.837059974670396 | 31.8190574645996 | 106.6803514957428 | |
3.07441967628593 | 29.0965259075165 | 29.8137545585633 | 31.8168520927429 | 31.7060291767121 | 3.07441967628593 | 179.0965259075165 | 54.8137545585633 | 31.8168520927429 | 106.7060291767121 | |
3.08161972236623 | 29.0934205055237 | 29.7954201698303 | 31.820285320282 | 31.7429661750794 | 3.08161972236623 | 179.0934205055237 | 54.7954201698303 | 31.820285320282 | 106.7429661750794 | |
3.08881976844652 | 29.0905773639679 | 29.7842502593994 | 31.8324148654938 | 31.7918777465821 | 3.08881976844652 | 179.0905773639679 | 54.7842502593994 | 31.8324148654938 | 106.7918777465821 | |
3.09601981452681 | 29.0882349014282 | 29.7815501689911 | 31.8564891815186 | 31.8533003330231 | 3.09601981452681 | 179.0882349014282 | 54.7815501689911 | 31.8564891815186 | 106.8533003330231 | |
3.10321986060711 | 29.0865302085877 | 29.7873020172119 | 31.8955779075623 | 31.9270253181458 | 3.10321986060711 | 179.0865302085877 | 54.7873020172119 | 31.8955779075623 | 106.92702531814581 | |
3.1104199066874 | 29.0855705738068 | 29.8000514507294 | 31.9518387317658 | 32.0111930370331 | 3.1104199066874 | 179.0855705738068 | 54.8000514507294 | 31.9518387317658 | 107.01119303703311 | |
3.1176199527677 | 29.0856420993805 | 29.817259311676 | 32.0256590843201 | 32.1020603179932 | 3.1176199527677 | 179.0856420993805 | 54.817259311676 | 32.0256590843201 | 107.10206031799319 | |
3.124819998848 | 29.0872216224671 | 29.8359215259552 | 32.1150839328766 | 32.1945011615753 | 3.124819998848 | 179.0872216224671 | 54.8359215259552 | 32.1150839328766 | 107.1945011615753 | |
3.13202004492829 | 29.0908694267273 | 29.8532545566559 | 32.2158336639405 | 32.2829127311707 | 3.13202004492829 | 179.0908694267273 | 54.8532545566559 | 32.2158336639405 | 107.2829127311707 | |
3.13922009100858 | 29.096919298172 | 29.8668622970581 | 32.3219895362854 | 32.3619782924652 | 3.13922009100858 | 179.096919298172 | 54.8668622970581 | 32.3219895362854 | 107.36197829246521 | |
3.14642013708888 | 29.1052281856537 | 29.8749208450318 | 32.4268817901612 | 32.4270486831665 | 3.14642013708888 | 179.1052281856537 | 54.874920845031795 | 32.4268817901612 | 107.4270486831665 | |
3.15362018316917 | 29.115092754364 | 29.8764646053314 | 32.5237572193146 | 32.4743628501892 | 3.15362018316917 | 179.115092754364 | 54.8764646053314 | 32.5237572193146 | 107.47436285018921 | |
3.16082022924947 | 29.1253387928009 | 29.8717081546784 | 32.6064229011536 | 32.5015187263489 | 3.16082022924947 | 179.1253387928009 | 54.8717081546784 | 32.6064229011536 | 107.5015187263489 | |
3.16802027532976 | 29.1346609592438 | 29.8619687557221 | 32.6700389385224 | 32.50812292099 | 3.16802027532976 | 179.1346609592438 | 54.8619687557221 | 32.6700389385224 | 107.50812292098999 | |
3.17522032141006 | 29.1419625282288 | 29.8491775989533 | 32.7118217945099 | 32.4959516525269 | 3.17522032141006 | 179.1419625282288 | 54.849177598953304 | 32.7118217945099 | 107.4959516525269 | |
3.18242036749035 | 29.1464924812317 | 29.835307598114 | 32.7312469482422 | 32.4683845043183 | 3.18242036749035 | 179.1464924812317 | 54.835307598114 | 32.7312469482422 | 107.4683845043183 | |
3.18962041357065 | 29.1479825973511 | 29.8220574855805 | 32.7296078205109 | 32.4294626712799 | 3.18962041357065 | 179.1479825973511 | 54.8220574855805 | 32.7296078205109 | 107.42946267127991 | |
3.19682045965094 | 29.1465997695923 | 29.8108518123627 | 32.7093780040741 | 32.3829352855683 | 3.19682045965094 | 179.14659976959229 | 54.8108518123627 | 32.7093780040741 | 107.3829352855683 | |
3.20402050573124 | 29.1429340839386 | 29.8026263713837 | 32.6737821102143 | 32.3320746421814 | 3.20402050573124 | 179.1429340839386 | 54.802626371383695 | 32.6737821102143 | 107.3320746421814 | |
3.21122055181153 | 29.1379034519196 | 29.7975659370423 | 32.6266050338745 | 32.2797238826752 | 3.21122055181153 | 179.1379034519196 | 54.7975659370423 | 32.6266050338745 | 107.2797238826752 | |
3.21842059789183 | 29.1325390338898 | 29.7950446605683 | 32.571816444397 | 32.2282731533051 | 3.21842059789183 | 179.1325390338898 | 54.7950446605683 | 32.571816444397 | 107.22827315330511 | |
3.22562064397212 | 29.1277170181275 | 29.7939598560333 | 32.5129270553589 | 32.1794211864472 | 3.22562064397212 | 179.1277170181275 | 54.7939598560333 | 32.5129270553589 | 107.1794211864472 | |
3.23282069005242 | 29.1238427162171 | 29.7932803630829 | 32.4524700641632 | 32.1339786052704 | 3.23282069005242 | 179.1238427162171 | 54.7932803630829 | 32.4524700641632 | 107.1339786052704 | |
3.24002073613271 | 29.1207075119019 | 29.792320728302 | 32.3922157287598 | 32.0920288562775 | 3.24002073613271 | 179.1207075119019 | 54.792320728302 | 32.3922157287598 | 107.0920288562775 | |
3.24722078221301 | 29.1176080703736 | 29.7907054424286 | 32.3334515094757 | 32.0534825325012 | 3.24722078221301 | 179.1176080703736 | 54.7907054424286 | 32.3334515094757 | 107.05348253250119 | |
3.2544208282933 | 29.1137218475342 | 29.788339138031 | 32.2773337364197 | 32.0182681083679 | 3.2544208282933 | 179.1137218475342 | 54.788339138031 | 32.2773337364197 | 107.01826810836789 | |
3.2616208743736 | 29.1084945201874 | 29.7855198383332 | 32.2246491909027 | 31.9862186908722 | 3.2616208743736 | 179.1084945201874 | 54.7855198383332 | 32.2246491909027 | 106.98621869087219 | |
3.26882092045389 | 29.1018605232239 | 29.7828912734986 | 32.1756064891815 | 31.9566488265991 | 3.26882092045389 | 179.1018605232239 | 54.7828912734986 | 32.1756064891815 | 106.95664882659909 | |
3.27602096653419 | 29.0941774845124 | 29.7811806201935 | 32.1298897266388 | 31.9282829761505 | 3.27602096653419 | 179.0941774845124 | 54.781180620193496 | 32.1298897266388 | 106.9282829761505 | |
3.28322101261448 | 29.0862023830414 | 29.7807991504669 | 32.0871412754059 | 31.8997323513031 | 3.28322101261448 | 179.0862023830414 | 54.780799150466905 | 32.0871412754059 | 106.8997323513031 | |
3.29042105869478 | 29.0789604187012 | 29.7817409038544 | 32.0470690727234 | 31.8700969219208 | 3.29042105869478 | 179.0789604187012 | 54.7817409038544 | 32.0470690727234 | 106.8700969219208 | |
3.29762110477507 | 29.0737330913544 | 29.7837257385254 | 32.0093333721161 | 31.8392455577851 | 3.29762110477507 | 179.0737330913544 | 54.783725738525405 | 32.0093333721161 | 106.8392455577851 | |
3.30482115085537 | 29.0718019008637 | 29.7863841056824 | 31.97340965271 | 31.8075358867645 | 3.30482115085537 | 179.0718019008637 | 54.7863841056824 | 31.97340965271 | 106.8075358867645 | |
3.31202119693566 | 29.0740668773651 | 29.789263010025 | 31.9387078285217 | 31.7755103111267 | 3.31202119693566 | 179.0740668773651 | 54.789263010024996 | 31.9387078285217 | 106.7755103111267 | |
3.31922124301596 | 29.0806829929352 | 29.7918736934662 | 31.9050908088684 | 31.7439436912537 | 3.31922124301596 | 179.0806829929352 | 54.7918736934662 | 31.9050908088684 | 106.7439436912537 | |
3.32642128909625 | 29.0909826755524 | 29.7939658164978 | 31.8730413913727 | 31.7140460014343 | 3.32642128909625 | 179.0909826755524 | 54.7939658164978 | 31.8730413913727 | 106.7140460014343 | |
3.33362133517655 | 29.1037023067475 | 29.7958195209503 | 31.8433344364166 | 31.6875040531159 | 3.33362133517655 | 179.1037023067475 | 54.7958195209503 | 31.8433344364166 | 106.6875040531159 | |
3.34082138125684 | 29.1172742843628 | 29.7981560230255 | 31.8164706230164 | 31.6660106182099 | 3.34082138125684 | 179.1172742843628 | 54.7981560230255 | 31.8164706230164 | 106.6660106182099 | |
3.34802142733714 | 29.1301369667053 | 29.8017144203186 | 31.7925989627838 | 31.6505789756775 | 3.34802142733714 | 179.1301369667053 | 54.8017144203186 | 31.7925989627838 | 106.6505789756775 | |
3.35522147341743 | 29.1410028934479 | 29.8068821430207 | 31.7717671394348 | 31.6411375999451 | 3.35522147341743 | 179.1410028934479 | 54.8068821430207 | 31.7717671394348 | 106.6411375999451 | |
3.36242151949773 | 29.1490554809571 | 29.8136830329895 | 31.7542850971222 | 31.6368043422699 | 3.36242151949773 | 179.1490554809571 | 54.8136830329895 | 31.7542850971222 | 106.6368043422699 | |
3.36962156557802 | 29.1542291641236 | 29.821902513504 | 31.7405045032501 | 31.6362023353577 | 3.36962156557802 | 179.1542291641236 | 54.821902513504 | 31.7405045032501 | 106.6362023353577 | |
3.37682161165832 | 29.1571438312531 | 29.8310935497284 | 31.7303895950318 | 31.637716293335 | 3.37682161165832 | 179.1571438312531 | 54.8310935497284 | 31.7303895950318 | 106.637716293335 | |
3.38402165773861 | 29.1588604450226 | 29.8404633998871 | 31.7234039306641 | 31.6395819187165 | 3.38402165773861 | 179.1588604450226 | 54.8404633998871 | 31.7234039306641 | 106.6395819187165 | |
3.39122170381891 | 29.1605532169342 | 29.8489212989807 | 31.7189574241638 | 31.6399157047272 | 3.39122170381891 | 179.1605532169342 | 54.8489212989807 | 31.7189574241638 | 106.6399157047272 | |
3.3984217498992 | 29.1632175445557 | 29.855227470398 | 31.7168056964874 | 31.6370666027069 | 3.3984217498992 | 179.1632175445557 | 54.855227470398 | 31.7168056964874 | 106.6370666027069 | |
3.4056217959795 | 29.167515039444 | 29.8583567142487 | 31.7170202732086 | 31.6300928592682 | 3.4056217959795 | 179.167515039444 | 54.8583567142487 | 31.7170202732086 | 106.6300928592682 | |
3.41282184205979 | 29.1736721992493 | 29.8576951026917 | 31.7194938659668 | 31.6191852092743 | 3.41282184205979 | 179.1736721992493 | 54.8576951026917 | 31.7194938659668 | 106.61918520927429 | |
3.42002188814009 | 29.1812539100647 | 29.853218793869 | 31.7236065864563 | 31.6055655479431 | 3.42002188814009 | 179.1812539100647 | 54.853218793869004 | 31.7236065864563 | 106.6055655479431 | |
3.42722193422038 | 29.1891872882843 | 29.8455357551575 | 31.7283630371094 | 31.5911412239075 | 3.42722193422038 | 179.1891872882843 | 54.8455357551575 | 31.7283630371094 | 106.5911412239075 | |
3.43442198030068 | 29.196035861969 | 29.8358380794525 | 31.7329108715058 | 31.5779209136963 | 3.43442198030068 | 179.196035861969 | 54.8358380794525 | 31.7329108715058 | 106.5779209136963 | |
3.44162202638097 | 29.2005062103272 | 29.8256874084473 | 31.7367851734162 | 31.5676689147949 | 3.44162202638097 | 179.2005062103272 | 54.8256874084473 | 31.7367851734162 | 106.5676689147949 | |
3.44882207246127 | 29.2017996311188 | 29.8169434070587 | 31.7396819591522 | 31.5618455410004 | 3.44882207246127 | 179.2017996311188 | 54.8169434070587 | 31.7396819591522 | 106.5618455410004 | |
3.45602211854156 | 29.1996896266937 | 29.8115015029907 | 31.7414164543152 | 31.5615057945252 | 3.45602211854156 | 179.1996896266937 | 54.8115015029907 | 31.7414164543152 | 106.5615057945252 | |
3.46322216462186 | 29.1943728923798 | 29.8110365867615 | 31.7418873310089 | 31.5669596195221 | 3.46322216462186 | 179.1943728923798 | 54.8110365867615 | 31.7418873310089 | 106.5669596195221 | |
3.47042221070215 | 29.186362028122 | 29.8165738582611 | 31.7414700984955 | 31.5777182579041 | 3.47042221070215 | 179.186362028122 | 54.8165738582611 | 31.7414700984955 | 106.5777182579041 | |
3.47762225678245 | 29.1765868663788 | 29.8280119895935 | 31.7410171031952 | 31.5925776958466 | 3.47762225678245 | 179.17658686637878 | 54.8280119895935 | 31.7410171031952 | 106.5925776958466 | |
3.48482230286274 | 29.1663646697998 | 29.8441708087921 | 31.7417204380036 | 31.6101372241974 | 3.48482230286274 | 179.1663646697998 | 54.8441708087921 | 31.7417204380036 | 106.6101372241974 | |
3.49202234894304 | 29.1571855545044 | 29.8632085323334 | 31.7447721958161 | 31.6291391849518 | 3.49202234894304 | 179.1571855545044 | 54.8632085323334 | 31.7447721958161 | 106.6291391849518 | |
3.49922239502333 | 29.1503608226776 | 29.8831224441529 | 31.7512631416321 | 31.6485166549683 | 3.49922239502333 | 179.1503608226776 | 54.8831224441529 | 31.7512631416321 | 106.6485166549683 | |
3.50642244110363 | 29.1467308998108 | 29.9020648002625 | 31.7622125148773 | 31.667298078537 | 3.50642244110363 | 179.1467308998108 | 54.9020648002625 | 31.7622125148773 | 106.667298078537 | |
3.51362248718392 | 29.146671295166 | 29.918521642685 | 31.7785203456879 | 31.6848278045655 | 3.51362248718392 | 179.14667129516602 | 54.918521642685 | 31.7785203456879 | 106.6848278045655 | |
3.52082253326422 | 29.1502594947815 | 29.9314737319946 | 31.8007230758667 | 31.700873374939 | 3.52082253326422 | 179.1502594947815 | 54.9314737319946 | 31.8007230758667 | 106.700873374939 | |
3.52802257934451 | 29.157167673111 | 29.9407362937927 | 31.8287968635559 | 31.7155838012696 | 3.52802257934451 | 179.157167673111 | 54.940736293792696 | 31.8287968635559 | 106.7155838012696 | |
3.5352226254248 | 29.1665494441986 | 29.9470663070679 | 31.8621814250946 | 31.7291617393494 | 3.5352226254248 | 179.1665494441986 | 54.9470663070679 | 31.8621814250946 | 106.7291617393494 | |
3.5424226715051 | 29.1769981384278 | 29.9517452716828 | 31.9000124931336 | 31.7416071891785 | 3.5424226715051 | 179.1769981384278 | 54.951745271682796 | 31.9000124931336 | 106.7416071891785 | |
3.5496227175854 | 29.1869044303894 | 29.9560129642487 | 31.9411158561707 | 31.7528069019318 | 3.5496227175854 | 179.1869044303894 | 54.9560129642487 | 31.9411158561707 | 106.7528069019318 | |
3.55682276366569 | 29.1948437690735 | 29.9605429172516 | 31.9839060306549 | 31.7627608776093 | 3.55682276366569 | 179.1948437690735 | 54.9605429172516 | 31.9839060306549 | 106.7627608776093 | |
3.56402280974598 | 29.1998863220215 | 29.9653291702271 | 32.0264220237732 | 31.7716002464295 | 3.56402280974598 | 179.19988632202148 | 54.9653291702271 | 32.0264220237732 | 106.7716002464295 | |
3.57122285582628 | 29.20161485672 | 29.9699425697327 | 32.0666432380676 | 31.7793309688568 | 3.57122285582628 | 179.20161485672 | 54.9699425697327 | 32.0666432380676 | 106.7793309688568 | |
3.57842290190657 | 29.2002081871033 | 29.9737572669983 | 32.1031212806702 | 31.785786151886 | 3.57842290190657 | 179.2002081871033 | 54.973757266998305 | 32.1031212806702 | 106.785786151886 |