Selected Cell
Cell:
Value:
Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
A
B
C
D
E
F
G
H
I
J
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
A
B
C
D
E
F
G
H
I
J
K
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
A
B
C
D
E
F
G
H
I
J
K
L
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
A
B
C
D
E
F
G
H
I
J
K
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
2.14561373192789 | 29.9089670181275 | 30.9873044490814 | 61.5114212036133 | 59.4152331352234 | 2.14561373192789 | 179.9089670181275 | 55.9873044490814 | 61.5114212036133 | 134.4152331352234 | |
2.15281377800818 | 29.8725664615631 | 30.936074256897 | 59.0801000595093 | 56.7487537860871 | 2.15281377800818 | 179.8725664615631 | 55.936074256897 | 59.0801000595093 | 131.7487537860871 | |
2.16001382408848 | 29.8375129699707 | 30.8822095394135 | 56.3880324363709 | 53.9259612560273 | 2.16001382408848 | 179.8375129699707 | 55.882209539413495 | 56.3880324363709 | 128.9259612560273 | |
2.16721387016877 | 29.8037111759186 | 30.828595161438 | 53.5800158977509 | 51.0901033878327 | 2.16721387016877 | 179.8037111759186 | 55.828595161438 | 53.5800158977509 | 126.0901033878327 | |
2.17441391624907 | 29.7713696956635 | 30.7774424552918 | 50.7918357849121 | 48.3665227890015 | 2.17441391624907 | 179.7713696956635 | 55.777442455291805 | 50.7918357849121 | 123.3665227890015 | |
2.18161396232936 | 29.7409415245056 | 30.730152130127 | 48.141747713089 | 45.8572864532471 | 2.18161396232936 | 179.74094152450562 | 55.730152130126996 | 48.141747713089 | 120.8572864532471 | |
2.18881400840966 | 29.7127664089203 | 30.6873679161072 | 45.726090669632 | 43.6383068561554 | 2.18881400840966 | 179.7127664089203 | 55.6873679161072 | 45.726090669632 | 118.6383068561554 | |
2.19601405448995 | 29.6869337558747 | 30.6491613388062 | 43.609619140625 | 41.7512357234955 | 2.19601405448995 | 179.6869337558747 | 55.649161338806195 | 43.609619140625 | 116.7512357234955 | |
2.20321410057025 | 29.6635210514069 | 30.615109205246 | 41.8163478374481 | 40.1974022388458 | 2.20321410057025 | 179.6635210514069 | 55.615109205246 | 41.8163478374481 | 115.1974022388458 | |
2.21041414665054 | 29.6426773071289 | 30.584317445755 | 40.3325080871582 | 38.9440655708313 | 2.21041414665054 | 179.6426773071289 | 55.584317445755005 | 40.3325080871582 | 113.9440655708313 | |
2.21761419273084 | 29.6244084835053 | 30.5556833744049 | 39.1219854354859 | 37.9418730735779 | 2.21761419273084 | 179.6244084835053 | 55.5556833744049 | 39.1219854354859 | 112.94187307357791 | |
2.22481423881113 | 29.6083867549896 | 30.5282890796662 | 38.1436586380005 | 37.1421933174134 | 2.22481423881113 | 179.6083867549896 | 55.5282890796662 | 38.1436586380005 | 112.1421933174134 | |
2.23201428489143 | 29.5938789844513 | 30.5015563964844 | 37.3606681823731 | 36.5052402019501 | 2.23201428489143 | 179.5938789844513 | 55.5015563964844 | 37.3606681823731 | 111.5052402019501 | |
2.23921433097172 | 29.580157995224 | 30.4753124713898 | 36.7406308650971 | 35.9992444515228 | 2.23921433097172 | 179.580157995224 | 55.4753124713898 | 36.7406308650971 | 110.9992444515228 | |
2.24641437705202 | 29.5669496059418 | 30.4495215415955 | 36.25328540802 | 35.5974555015564 | 2.24641437705202 | 179.5669496059418 | 55.4495215415955 | 36.25328540802 | 110.5974555015564 | |
2.25361442313231 | 29.5543670654297 | 30.4240882396698 | 35.870486497879 | 35.27752161026 | 2.25361442313231 | 179.5543670654297 | 55.4240882396698 | 35.870486497879 | 110.27752161026001 | |
2.2608144692126 | 29.5424520969391 | 30.3988635540009 | 35.5679035186768 | 35.0225150585175 | 2.2608144692126 | 179.5424520969391 | 55.3988635540009 | 35.5679035186768 | 110.0225150585175 | |
2.2680145152929 | 29.5310258865357 | 30.3738296031952 | 35.3263139724732 | 34.8213076591492 | 2.2680145152929 | 179.5310258865357 | 55.373829603195205 | 35.3263139724732 | 109.8213076591492 | |
2.27521456137319 | 29.5197188854218 | 30.3492605686188 | 35.1315259933472 | 34.6678137779236 | 2.27521456137319 | 179.5197188854218 | 55.3492605686188 | 35.1315259933472 | 109.6678137779236 | |
2.28241460745349 | 29.5083522796631 | 30.3256928920746 | 34.9736392498017 | 34.5600426197052 | 2.28241460745349 | 179.5083522796631 | 55.3256928920746 | 34.9736392498017 | 109.5600426197052 | |
2.28961465353378 | 29.4969022274018 | 30.3037464618683 | 34.8463892936707 | 34.4998002052307 | 2.28961465353378 | 179.4969022274018 | 55.3037464618683 | 34.8463892936707 | 109.4998002052307 | |
2.29681469961408 | 29.48517203331 | 30.2838802337647 | 34.7465455532074 | 34.4929873943329 | 2.29681469961408 | 179.48517203331 | 55.283880233764705 | 34.7465455532074 | 109.4929873943329 | |
2.30401474569437 | 29.4725835323334 | 30.2663326263428 | 34.6734941005707 | 34.5499038696289 | 2.30401474569437 | 179.4725835323334 | 55.2663326263428 | 34.6734941005707 | 109.5499038696289 | |
2.31121479177467 | 29.4584572315216 | 30.2511811256409 | 34.6290946006775 | 34.6852540969849 | 2.31121479177467 | 179.4584572315216 | 55.2511811256409 | 34.6290946006775 | 109.68525409698489 | |
2.31841483785496 | 29.4425368309021 | 30.2383244037628 | 34.6176862716675 | 34.9177539348603 | 2.31841483785496 | 179.4425368309021 | 55.2383244037628 | 34.6176862716675 | 109.9177539348603 | |
2.32561488393526 | 29.4250786304474 | 30.2275776863098 | 34.6461832523346 | 35.2692544460297 | 2.32561488393526 | 179.4250786304474 | 55.2275776863098 | 34.6461832523346 | 110.26925444602969 | |
2.33281493001555 | 29.4065117835999 | 30.2186369895935 | 34.7237944602967 | 35.7630372047425 | 2.33281493001555 | 179.4065117835999 | 55.2186369895935 | 34.7237944602967 | 110.7630372047425 | |
2.34001497609585 | 29.3870568275452 | 30.2109718322754 | 34.8613202571869 | 36.4210546016693 | 2.34001497609585 | 179.3870568275452 | 55.210971832275405 | 34.8613202571869 | 111.4210546016693 | |
2.34721502217614 | 29.3667137622833 | 30.2038550376892 | 35.0701451301575 | 37.2600913047791 | 2.34721502217614 | 179.3667137622833 | 55.203855037689195 | 35.0701451301575 | 112.26009130477911 | |
2.35441506825644 | 29.3457686901093 | 30.196487903595 | 35.3608191013336 | 38.287228345871 | 2.35441506825644 | 179.3457686901093 | 55.196487903595 | 35.3608191013336 | 113.287228345871 | |
2.36161511433673 | 29.3248116970062 | 30.1881611347199 | 35.7413053512573 | 39.4954681396485 | 2.36161511433673 | 179.3248116970062 | 55.1881611347199 | 35.7413053512573 | 114.4954681396485 | |
2.36881516041703 | 29.304450750351 | 30.178451538086 | 36.2152874469757 | 40.8605635166168 | 2.36881516041703 | 179.304450750351 | 55.178451538086 | 36.2152874469757 | 115.86056351661679 | |
2.37601520649732 | 29.2849779129029 | 30.1671743392945 | 36.780446767807 | 42.3397779464722 | 2.37601520649732 | 179.2849779129029 | 55.167174339294505 | 36.780446767807 | 117.3397779464722 | |
2.38321525257762 | 29.2665362358093 | 30.1542103290558 | 37.4271631240845 | 43.8732743263245 | 2.38321525257762 | 179.2665362358093 | 55.1542103290558 | 37.4271631240845 | 118.87327432632449 | |
2.39041529865791 | 29.2495548725128 | 30.1396191120148 | 38.1378412246704 | 45.3883290290833 | 2.39041529865791 | 179.2495548725128 | 55.1396191120148 | 38.1378412246704 | 120.38832902908331 | |
2.39761534473821 | 29.2349874973297 | 30.1238775253296 | 38.887083530426 | 46.8057870864868 | 2.39761534473821 | 179.2349874973297 | 55.123877525329604 | 38.887083530426 | 121.8057870864868 | |
2.4048153908185 | 29.2237639427185 | 30.1080167293549 | 39.6431565284729 | 48.0481564998627 | 2.4048153908185 | 179.2237639427185 | 55.1080167293549 | 39.6431565284729 | 123.0481564998627 | |
2.4120154368988 | 29.2163193225861 | 30.0934791564942 | 40.3707027435303 | 49.047863483429 | 2.4120154368988 | 179.2163193225861 | 55.0934791564942 | 40.3707027435303 | 124.047863483429 | |
2.41921548297909 | 29.2125463485718 | 30.0815582275391 | 41.0341262817383 | 49.7544825077057 | 2.41921548297909 | 179.2125463485718 | 55.081558227539105 | 41.0341262817383 | 124.7544825077057 | |
2.42641552905939 | 29.2120695114136 | 30.0729811191559 | 41.6012585163117 | 50.1400232315064 | 2.42641552905939 | 179.2120695114136 | 55.0729811191559 | 41.6012585163117 | 125.1400232315064 | |
2.43361557513968 | 29.2145371437073 | 30.0677299499512 | 42.0465469360352 | 50.201416015625 | 2.43361557513968 | 179.2145371437073 | 55.0677299499512 | 42.0465469360352 | 125.201416015625 | |
2.44081562121998 | 29.2192995548249 | 30.0651371479035 | 42.3537075519562 | 49.9598920345307 | 2.44081562121998 | 179.2192995548249 | 55.0651371479035 | 42.3537075519562 | 124.9598920345307 | |
2.44801566730027 | 29.2250990867615 | 30.0640523433686 | 42.5173342227936 | 49.4573652744293 | 2.44801566730027 | 179.2250990867615 | 55.0640523433686 | 42.5173342227936 | 124.45736527442929 | |
2.45521571338057 | 29.230409860611 | 30.0629317760468 | 42.5432562828064 | 48.7502932548523 | 2.45521571338057 | 179.230409860611 | 55.062931776046796 | 42.5432562828064 | 123.7502932548523 | |
2.46241575946086 | 29.2340278625488 | 30.0600647926331 | 42.4472868442536 | 47.9022026062012 | 2.46241575946086 | 179.2340278625488 | 55.0600647926331 | 42.4472868442536 | 122.9022026062012 | |
2.46961580554116 | 29.2355358600617 | 30.0540089607239 | 42.2528028488159 | 46.9762265682221 | 2.46961580554116 | 179.2355358600617 | 55.0540089607239 | 42.2528028488159 | 121.9762265682221 | |
2.47681585162145 | 29.2351841926575 | 30.0438642501831 | 41.9875800609589 | 46.0287392139435 | 2.47681585162145 | 179.2351841926575 | 55.0438642501831 | 41.9875800609589 | 121.02873921394351 | |
2.48401589770175 | 29.2334854602814 | 30.0295770168305 | 41.6803658008576 | 45.1046705245972 | 2.48401589770175 | 179.2334854602814 | 55.0295770168305 | 41.6803658008576 | 120.1046705245972 | |
2.49121594378204 | 29.2309522628784 | 30.0119400024414 | 41.3577198982239 | 44.2350804805756 | 2.49121594378204 | 179.2309522628784 | 55.0119400024414 | 41.3577198982239 | 119.23508048057559 | |
2.49841598986234 | 29.2281091213227 | 29.9924671649933 | 41.0412788391113 | 43.4367179870606 | 2.49841598986234 | 179.2281091213227 | 54.9924671649933 | 41.0412788391113 | 118.4367179870606 | |
2.50561603594263 | 29.2255580425263 | 29.9730360507965 | 40.7457351684571 | 42.7134811878205 | 2.50561603594263 | 179.2255580425263 | 54.9730360507965 | 40.7457351684571 | 117.71348118782049 | |
2.51281608202293 | 29.2237877845764 | 29.955267906189 | 40.4778838157654 | 42.0592665672302 | 2.51281608202293 | 179.2237877845764 | 54.955267906189 | 40.4778838157654 | 117.0592665672302 | |
2.52001612810322 | 29.2231023311615 | 29.9400806427002 | 40.2369141578675 | 41.4616346359253 | 2.52001612810322 | 179.2231023311615 | 54.940080642700195 | 40.2369141578675 | 116.4616346359253 | |
2.52721617418352 | 29.223495721817 | 29.9277126789093 | 40.015858411789 | 40.905624628067 | 2.52721617418352 | 179.223495721817 | 54.9277126789093 | 40.015858411789 | 115.905624628067 | |
2.53441622026381 | 29.2246818542481 | 29.9181401729584 | 39.8038327693939 | 40.3772830963135 | 2.53441622026381 | 179.2246818542481 | 54.9181401729584 | 39.8038327693939 | 115.3772830963135 | |
2.54161626634411 | 29.2262434959412 | 29.9113512039185 | 39.5887732505799 | 39.8661613464356 | 2.54161626634411 | 179.2262434959412 | 54.9113512039185 | 39.5887732505799 | 114.8661613464356 | |
2.5488163124244 | 29.2279481887818 | 29.9073219299317 | 39.3599927425385 | 39.3665432929993 | 2.5488163124244 | 179.2279481887818 | 54.9073219299317 | 39.3599927425385 | 114.3665432929993 | |
2.5560163585047 | 29.2298913002014 | 29.905766248703 | 39.11013007164 | 38.8775408267975 | 2.5560163585047 | 179.2298913002014 | 54.905766248703 | 39.11013007164 | 113.8775408267975 | |
2.56321640458499 | 29.232519865036 | 29.906165599823 | 38.8365030288696 | 38.4023129940033 | 2.56321640458499 | 179.232519865036 | 54.906165599823 | 38.8365030288696 | 113.4023129940033 | |
2.57041645066529 | 29.23623919487 | 29.9081146717072 | 38.5415732860565 | 37.9469573497772 | 2.57041645066529 | 179.23623919487 | 54.908114671707196 | 38.5415732860565 | 112.9469573497772 | |
2.57761649674558 | 29.2410671710968 | 29.9115836620331 | 38.2327675819397 | 37.519371509552 | 2.57761649674558 | 179.2410671710968 | 54.911583662033095 | 38.2327675819397 | 112.519371509552 | |
2.58481654282588 | 29.2467713356018 | 29.9166977405548 | 37.9213988780976 | 37.1279537677765 | 2.58481654282588 | 179.2467713356018 | 54.916697740554795 | 37.9213988780976 | 112.1279537677765 | |
2.59201658890617 | 29.2530834674835 | 29.9233496189118 | 37.62087225914 | 36.7802381515503 | 2.59201658890617 | 179.2530834674835 | 54.9233496189118 | 37.62087225914 | 111.7802381515503 | |
2.59921663498647 | 29.2597115039826 | 29.9309730529785 | 37.3445928096771 | 36.4817976951599 | 2.59921663498647 | 179.2597115039826 | 54.9309730529785 | 37.3445928096771 | 111.4817976951599 | |
2.60641668106676 | 29.2660772800446 | 29.9387514591217 | 37.1043682098389 | 36.2356543540955 | 2.60641668106676 | 179.2660772800446 | 54.938751459121704 | 37.1043682098389 | 111.2356543540955 | |
2.61361672714706 | 29.2711317539215 | 29.9460768699646 | 36.9094729423523 | 36.0424995422363 | 2.61361672714706 | 179.2711317539215 | 54.9460768699646 | 36.9094729423523 | 111.0424995422363 | |
2.62081677322735 | 29.2737007141113 | 29.9527823925019 | 36.7663085460663 | 35.9010338783264 | 2.62081677322735 | 179.2737007141113 | 54.9527823925019 | 36.7663085460663 | 110.9010338783264 | |
2.62801681930765 | 29.2731463909149 | 29.9590647220612 | 36.6780638694763 | 35.8081817626953 | 2.62801681930765 | 179.2731463909149 | 54.9590647220612 | 36.6780638694763 | 110.8081817626953 | |
2.63521686538794 | 29.2696714401245 | 29.9652338027954 | 36.6443872451782 | 35.7588410377503 | 2.63521686538794 | 179.2696714401245 | 54.965233802795396 | 36.6443872451782 | 110.7588410377503 | |
2.64241691146824 | 29.2640388011933 | 29.9715638160706 | 36.6611540317536 | 35.7455909252167 | 2.64241691146824 | 179.2640388011933 | 54.9715638160706 | 36.6611540317536 | 110.7455909252167 | |
2.64961695754853 | 29.2571187019348 | 29.9782633781433 | 36.7205917835236 | 35.7588648796082 | 2.64961695754853 | 179.25711870193481 | 54.978263378143296 | 36.7205917835236 | 110.7588648796082 | |
2.65681700362883 | 29.2498230934143 | 29.9855172634125 | 36.8119597434998 | 35.7878088951111 | 2.65681700362883 | 179.2498230934143 | 54.985517263412504 | 36.8119597434998 | 110.7878088951111 | |
2.66401704970912 | 29.2431592941284 | 29.9935162067414 | 36.9223594665528 | 35.8210921287537 | 2.66401704970912 | 179.2431592941284 | 54.993516206741404 | 36.9223594665528 | 110.82109212875369 | |
2.67121709578942 | 29.238224029541 | 30.0022661685944 | 37.0375037193299 | 35.8477711677551 | 2.67121709578942 | 179.23822402954102 | 55.0022661685944 | 37.0375037193299 | 110.8477711677551 | |
2.67841714186971 | 29.2358636856079 | 30.0115525722504 | 37.1425449848175 | 35.8578503131867 | 2.67841714186971 | 179.2358636856079 | 55.0115525722504 | 37.1425449848175 | 110.8578503131867 | |
2.68561718795 | 29.2363345623017 | 30.02108335495 | 37.2231602668762 | 35.8429789543152 | 2.68561718795 | 179.2363345623017 | 55.02108335495 | 37.2231602668762 | 110.8429789543152 | |
2.6928172340303 | 29.2394280433655 | 30.0305962562561 | 37.2669458389282 | 35.7973337173462 | 2.6928172340303 | 179.2394280433655 | 55.0305962562561 | 37.2669458389282 | 110.79733371734619 | |
2.70001728011059 | 29.2447745800019 | 30.0398707389832 | 37.2649729251862 | 35.7184588909149 | 2.70001728011059 | 179.2447745800019 | 55.0398707389832 | 37.2649729251862 | 110.7184588909149 | |
2.70721732619089 | 29.2520642280579 | 30.0485253334046 | 37.2127234935761 | 35.6073617935181 | 2.70721732619089 | 179.2520642280579 | 55.0485253334046 | 37.2127234935761 | 110.6073617935181 | |
2.71441737227118 | 29.2609512805939 | 30.0558865070343 | 37.1101319789887 | 35.4678928852082 | 2.71441737227118 | 179.2609512805939 | 55.0558865070343 | 37.1101319789887 | 110.4678928852082 | |
2.72161741835148 | 29.2708456516266 | 30.0611317157746 | 36.9609355926514 | 35.3057146072388 | 2.72161741835148 | 179.2708456516266 | 55.0611317157746 | 36.9609355926514 | 110.3057146072388 | |
2.72881746443177 | 29.2809426784516 | 30.0635695457459 | 36.7718279361725 | 35.127204656601 | 2.72881746443177 | 179.2809426784516 | 55.0635695457459 | 36.7718279361725 | 110.12720465660101 | |
2.73601751051207 | 29.2904615402222 | 30.0627291202545 | 36.5516662597656 | 34.938782453537 | 2.73601751051207 | 179.2904615402222 | 55.0627291202545 | 36.5516662597656 | 109.938782453537 | |
2.74321755659236 | 29.2988300323487 | 30.0582110881806 | 36.3105475902558 | 34.7465515136719 | 2.74321755659236 | 179.2988300323487 | 55.0582110881806 | 36.3105475902558 | 109.7465515136719 | |
2.75041760267266 | 29.3055236339569 | 30.0495803356171 | 36.0586404800415 | 34.5558881759644 | 2.75041760267266 | 179.3055236339569 | 55.0495803356171 | 36.0586404800415 | 109.5558881759644 | |
2.75761764875295 | 29.3098151683808 | 30.0367653369904 | 35.8049631118775 | 34.3709826469422 | 2.75761764875295 | 179.3098151683808 | 55.0367653369904 | 35.8049631118775 | 109.3709826469422 | |
2.76481769483325 | 29.3108999729157 | 30.0204873085022 | 35.5565965175629 | 34.1946661472321 | 2.76481769483325 | 179.3108999729157 | 55.0204873085022 | 35.5565965175629 | 109.1946661472321 | |
2.77201774091354 | 29.3084561824799 | 30.0022423267365 | 35.3186547756195 | 34.0284526348114 | 2.77201774091354 | 179.30845618247992 | 55.0022423267365 | 35.3186547756195 | 109.0284526348114 | |
2.77921778699384 | 29.3029844760895 | 29.9838662147522 | 35.0944995880127 | 33.8730156421662 | 2.77921778699384 | 179.3029844760895 | 54.9838662147522 | 35.0944995880127 | 108.8730156421662 | |
2.78641783307413 | 29.2956233024597 | 29.966801404953 | 34.885984659195 | 33.7286233901978 | 2.78641783307413 | 179.2956233024597 | 54.966801404953 | 34.885984659195 | 108.7286233901978 | |
2.79361787915443 | 29.2874753475189 | 29.9520015716553 | 34.6935749053955 | 33.5951328277588 | 2.79361787915443 | 179.2874753475189 | 54.9520015716553 | 34.6935749053955 | 108.5951328277588 | |
2.80081792523472 | 29.279088973999 | 29.9401819705963 | 34.5165491104126 | 33.4717512130738 | 2.80081792523472 | 179.279088973999 | 54.9401819705963 | 34.5165491104126 | 108.4717512130738 | |
2.80801797131502 | 29.2707026004791 | 29.9318671226502 | 34.3535006046295 | 33.3570539951325 | 2.80801797131502 | 179.2707026004791 | 54.9318671226502 | 34.3535006046295 | 108.3570539951325 | |
2.81521801739531 | 29.2625904083252 | 29.9270272254944 | 34.2027604579926 | 33.2492828369141 | 2.81521801739531 | 179.2625904083252 | 54.9270272254944 | 34.2027604579926 | 108.24928283691409 | |
2.82241806347561 | 29.2550265789032 | 29.924738407135 | 34.0625762939453 | 33.146995306015 | 2.82241806347561 | 179.2550265789032 | 54.924738407134996 | 34.0625762939453 | 108.146995306015 | |
2.8296181095559 | 29.2478621006012 | 29.9233138561249 | 33.931177854538 | 33.0495178699494 | 2.8296181095559 | 179.2478621006012 | 54.9233138561249 | 33.931177854538 | 108.0495178699494 | |
2.8368181556362 | 29.240483045578 | 29.9208998680115 | 33.8068962097168 | 32.9568862915039 | 2.8368181556362 | 179.240483045578 | 54.9208998680115 | 33.8068962097168 | 107.9568862915039 | |
2.84401820171649 | 29.2322516441345 | 29.9159646034241 | 33.6882770061493 | 32.8694462776184 | 2.84401820171649 | 179.2322516441345 | 54.9159646034241 | 33.6882770061493 | 107.86944627761841 | |
2.85121824779679 | 29.2231798171997 | 29.9074590206146 | 33.5742712020874 | 32.7876210212708 | 2.85121824779679 | 179.2231798171997 | 54.907459020614596 | 33.5742712020874 | 107.78762102127081 | |
2.85841829387708 | 29.2140543460846 | 29.8949778079987 | 33.4643185138703 | 32.711786031723 | 2.85841829387708 | 179.2140543460846 | 54.8949778079987 | 33.4643185138703 | 107.711786031723 |