Testing thiamine metabolites in Col and prr7prr9

Selected Cell

Cell:

Value:

Read.Me Sheet1 Results-std curve Repeat for thiamin Graph-stats Graph-stats (2) Chromatogram

Testing thiamine vitamers in plant samples from study L&H3
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites

Analysis Protocol	From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight.

Sheets of this workbook	Contents
Sheet1	Raw data and sampling plan
Results-std curve	Data for individual replicates and standard curve
Repeat for thiamin
Graph-stats	Summary statistics, means, t-test statistics except thiamine, and final charts
Graph-stats (2)	Summary statistics, means, t-test statistics only for thiamine
Chromatogram	raw data traces

Credit
Experimental design, plant growth and sampling	Yin Hoon Chew, Andrew Millar group University of Edinburgh
Analytical design, execution and data analysis	Michael Moulin, Teresa Fitzpatrick group University of Geneva
File Curation	Andrew Millar




numbering	Col				prr7,prr9					replicat	Col				prr7,prr9
27 EN	1	2	3	4	5	6	7	8		27 EN	1	2	3	4	1	2	3	4
28 ED	9	10	11	12	13	14	15	16		28 ED	1	2	3	4	1	2	3	4
28 EN	17	18	19	20	21	22	23	24		28 EN	1	2	3	4	1	2	3	4

mass tube	Col				prr7,prr9
27 EN	0.9438	0.9547	0.9455	0.9373	0.9437	0.9561	0.9486	0.9465
28 ED	0.9427	0.9391	0.9409	0.9434	0.9522	0.9437	0.9438	0.9442
28 EN	0.944	0.9421	0.9439	0.9465	0.9562	0.9436	0.9506	0.9442

mass tube+sample	Col				prr7,prr9
27 EN	1.0289	1.0105	0.992	0.9987	1.0115	1.0188	1.0039	1.0221
28 ED	1.0234	1.036	1.0222	1.0111	1.0581	0.9944	0.9984	1.0461
28 EN	1.0562	1.0187	1.0434	1.0493	1.0576	1.0668	1.0598	1.0178

mass sample	Col				prr7,prr9
27 EN	0.08509999999999995	0.05579999999999996	0.046499999999999986	0.06140000000000001	0.06780000000000008	0.06269999999999998	0.055300000000000016	0.0756
28 ED	0.0807000000000001	0.09689999999999999	0.08130000000000004	0.0677000000000001	0.1059	0.05069999999999997	0.05459999999999998	0.10189999999999999
28 EN	0.11220000000000008	0.07659999999999989	0.09950000000000014	0.10279999999999989	0.10140000000000005	0.12319999999999998	0.10920000000000007	0.0736

mass sample	Col				prr7,prr9
27 EN	300	200	200	200	200	200	200	300
28 ED	300	300	300	200	400	200	200	400
28 EN	300	300	300	300	40	400	400	300


	Extraxtion volume	Mass			Sample analysis order		TDP	TMP	THIA	Extraction volume	Mass
1	300	85.09999999999995			0.03125	pmol	98.93505859375	100.81101989746094	217.923583984375
2	200	55.79999999999996			0.0625	pmol	252.35	237.66	289.42
3	200	46.499999999999986			0.125	pmol	309.66	318.61	576.96
4	200	61.40000000000001			0.25	pmol	502.94	515.39	955.81
5	200	67.80000000000008			0.5	pmol	850.95	859.87	1269.7
6	200	62.699999999999974			1	pmol	1621.6	1673.7	2243.1
7	200	55.30000000000002			2	pmol	3490.9	3645	4747
8	300	75.6			4	pmol	6814.1	7077	9161
9	300	80.7000000000001			1	Col I	2180.39501953125	301.9862060546875	83.60588073730469	300	85.09999999999995
10	300	96.89999999999999			9	Col I	1917.8831787109375	375.14276123046875	132.87686157226562	300	80.7000000000001
11	300	81.30000000000004			17	Col I	2178.0859375	806.1314697265625	72.3534927368164	300	112.20000000000007
12	200	67.70000000000009			5	prr7,9 I	2514.615478515625	299.6667175292969	70.3182601928711	200	67.80000000000008
13	400	105.89999999999999			13	prr7,9 I	1365.0078125	1249.138671875	116.79225158691406	400	105.89999999999999
14	200	50.69999999999997			21	prr7,9 I	1654.855712890625	1042.51416015625	116.10993957519531	400	101.40000000000005
15	200	54.59999999999998			Standard	1	1645.3131103515625	1719.94091796875	2256.296630859375
16	400	101.89999999999999			2	Col II	2157.2509765625	176.75811767578125	54.220611572265625	200	55.79999999999996
17	300	112.20000000000007			10	Col II	1865.593994140625	616.8743286132812	59.84711456298828	300	96.89999999999999
18	200	76.5999999999999			18	Col II	2377.778564453125	465.9914245605469	59.2650146484375	200	76.5999999999999
19	300	99.50000000000014			6	prr7,9 II	2416.207275390625	603.93408203125	58.665550231933594	200	62.699999999999974
20	300	102.7999999999999			14	prr7,9 II	2289.3037109375	526.7593383789062	127.31964874267578	200	50.69999999999997
21	400	101.40000000000005			22	prr7,9 II	1362.8040771484375	1686.056884765625	129.24539184570312	400	123.19999999999997
22	400	123.19999999999997			Standard	0.5	808.2450561523438	831.448486328125	1122.8255615234375
23	400	109.20000000000007			3	Col III	2016.7115478515625	173.78077697753906	65.31547546386719	200	46.499999999999986
24	300	73.6			11	Col III	1981.958740234375	174.57398986816406	21.63164520263672	300	81.30000000000004
					19	Col III	2064.210693359375	473.5988464355469	64.27493286132812	300	99.50000000000014
					7	prr7,9 III	2498.947509765625	391.9966735839844	105.10193634033203	200	55.30000000000002
	Derivatization				15	prr7,9 III	2427.84375	588.3184204101562	63.29181671142578	200	54.59999999999998
	25 µL				23	prr7,9 III	1696.642333984375	1158.29	123.79141235351562	400	109.20000000000007
	injection	injection			Standard	1	1606.5908203125	1679.7308349609375	2153.149169921875
	20 µL				4	Col IV	2237.2646484375	125.17689514160156	43.60393142700195	200	61.40000000000001
					12	Col IV	2196.937255859375	269.585	42.28960418701172	200	67.70000000000009
					20	Col IV	2176.550048828125	417.441	73.04740905761719	300	102.7999999999999
					8	prr7,9 IV	2290.056396484375	659.33	345.0472412109375	300	75.6
					16	prr7,9 IV	1518.505859375	1229.6	95.14126586914062	400	101.89999999999999
					24	prr7,9 IV	2028.2176513671875	424.313	69.16194915771484	300	73.6
					Standard	0.25	447.85986328125	458.4064636230469	788.3421020507812
					Standard	0.03125	98.93505859375	100.81101989746094	217.923583984375



Sample analysis order		TDP	TMP	THIA	Extraction volume	Mass
1	Col I	2180.39501953125	301.9862060546875	83.60588073730469	3.0E-4	0.08509999999999995
2	Col II	2157.2509765625	176.75811767578125	54.220611572265625	1.9999999999999998E-4	0.05579999999999996
3	Col III	2016.7115478515625	173.78077697753906	65.31547546386719	1.9999999999999998E-4	0.046499999999999986
4	Col IV	2237.2646484375	125.17689514160156	43.60393142700195	1.9999999999999998E-4	0.06140000000000002
5	prr7,9 I	2514.615478515625	299.6667175292969	70.3182601928711	1.9999999999999998E-4	0.06780000000000008
6	prr7,9 II	2416.207275390625	603.93408203125	58.665550231933594	1.9999999999999998E-4	0.06269999999999998
7	prr7,9 III	2498.947509765625	391.9966735839844	105.10193634033203	1.9999999999999998E-4	0.05530000000000002				pmol	TDP	TMP	Thia
8	prr7,9 IV	2290.056396484375	659.33	345.0472412109375	3.0E-4	0.0756				3.125E-14	98.93505859375	100.81101989746094	217.923583984375
9	Col I	1917.8831787109375	375.14276123046875	132.87686157226562	3.0E-4	0.0807000000000001				6.25E-14	252.35	237.66	289.42
10	Col II	1865.593994140625	616.8743286132812	59.84711456298828	3.0E-4	0.0969				1.25E-13	309.66	318.61	576.96
11	Col III	1981.958740234375	174.57398986816406	21.63164520263672	3.0E-4	0.08130000000000004				2.5E-13	502.94	515.39	955.81
12	Col IV	2196.937255859375	269.585	42.28960418701172	1.9999999999999998E-4	0.0677000000000001				5.0E-13	850.95	859.87	1269.7
13	prr7,9 I	1365.0078125	1249.138671875	116.79225158691406	3.9999999999999996E-4	0.1059				1.0E-12	1621.6	1673.7	2243.1
14	prr7,9 II	2289.3037109375	526.7593383789062	127.31964874267578	1.9999999999999998E-4	0.05069999999999997				2.0E-12	3490.9	3645	4747
15	prr7,9 III	2427.84375	588.3184204101562	63.29181671142578	1.9999999999999998E-4	0.05459999999999998				4.0E-12	6814.1	7077	9161
16	prr7,9 IV	1518.505859375	1229.6	95.14126586914062	3.9999999999999996E-4	0.10189999999999999				Slope	1.685774878178111E15	1.7555770182597208E15	2.2371156417932975E15
17	Col I	2178.0859375	806.1314697265625	72.3534927368164	3.0E-4	0.11220000000000008				b	63.49	54.786	204.24
18	Col II	2377.778564453125	465.9914245605469	59.2650146484375	1.9999999999999998E-4	0.07659999999999989
19	Col III	2064.210693359375	473.5988464355469	64.27493286132812	3.0E-4	0.09950000000000014
20	Col IV	2176.550048828125	417.441	73.04740905761719	3.0E-4	0.1027999999999999
21	prr7,9 I	1654.855712890625	1042.51416015625	116.10993957519531	3.9999999999999996E-4	0.10140000000000005
22	prr7,9 II	1362.8040771484375	1686.056884765625	129.24539184570312	3.9999999999999996E-4	0.12319999999999998
23	prr7,9 III	1696.642333984375	1158.29	123.79141235351562	3.9999999999999996E-4	0.10920000000000007
24	prr7,9 IV	2028.2176513671875	424.313	69.16194915771484	3.0E-4	0.0736


Sample analysis order	pmol injected	TDP	TMP	THIA			Sample analysis order	pmol/µL	TDP	TMP	THIA
1	Col I	1.2557459758915614E-12	1.4080852248779928E-13	4.180294036865234E-14		27 EN	1	Col I	2.511491951783123E-7	2.8161704497559855E-8	8.360588073730468E-9
2	Col II	1.2420169523615854E-12	6.947693915285502E-14	2.7110305786132812E-14			2	Col II	2.4840339047231705E-7	1.3895387830571003E-8	5.422061157226562E-9
3	Col III	1.1586491014520826E-12	6.778100632434624E-14	3.265773773193359E-14			3	Col III	2.3172982029041653E-7	1.3556201264869248E-8	6.531547546386718E-9
4	Col IV	1.289480983835037E-12	4.009558931876374E-14	2.1801965713500978E-14			4	Col IV	2.5789619676700736E-7	8.019117863752749E-9	4.360393142700195E-9
5	prr7,9 I	1.4540052234997483E-12	1.3948731099934524E-13	3.515913009643555E-14			5	prr7,9 I	2.908010446999496E-7	2.7897462199869047E-8	7.0318260192871094E-9
6	prr7,9 II	1.3956295741773702E-12	3.1280204532160763E-13	2.93327751159668E-14			6	prr7,9 II	2.79125914835474E-7	6.256040906432153E-8	5.8665550231933595E-9
7	prr7,9 III	1.4447109998446106E-12	1.9207968096908483E-13	5.2550968170166016E-14			7	prr7,9 III	2.889421999689221E-7	3.8415936193816965E-8	1.0510193634033203E-8
8	prr7,9 IV	1.3207969968627843E-12	3.443562963698831E-13	1.7252362060546875E-13			8	prr7,9 IV	2.6415939937255685E-7	6.887125927397661E-8	3.450472412109375E-8
9	Col I	1.1000242100624132E-12	1.824794685157328E-13	6.643843078613281E-14		28 EN	9	Col I	2.2000484201248262E-7	3.6495893703146554E-8	1.328768615722656E-8
10	Col II	1.0690063171947585E-12	3.2017298174162225E-13	2.992355728149414E-14			10	Col II	2.138012634389517E-7	6.403459634832445E-8	5.9847114562988275E-9
11	Col III	1.1380337701483285E-12	6.823283092809465E-14	1.081582260131836E-14			11	Col III	2.2760675402966568E-7	1.364656618561893E-8	2.1631645202636718E-9
12	Col IV	1.2655588142143173E-12	1.2235236492952458E-13	2.114480209350586E-14			12	Col IV	2.5311176284286343E-7	2.4470472985904917E-8	4.228960418701171E-9
13	prr7,9 I	7.72059086505433E-13	6.803191540174895E-13	5.839612579345703E-14			13	prr7,9 I	1.5441181730108656E-7	1.360638308034979E-7	1.1679225158691405E-8
14	prr7,9 II	1.3203505045365442E-12	2.6884228573849006E-13	6.365982437133789E-14			14	prr7,9 II	2.640701009073088E-7	5.376845714769801E-8	1.2731964874267577E-8
15	prr7,9 III	1.4025323194727273E-12	3.039071569409353E-13	3.164590835571289E-14			15	prr7,9 III	2.8050646389454546E-7	6.078143138818706E-8	6.329181671142578E-9
16	prr7,9 IV	8.63113976966781E-13	6.691896668621105E-13	4.757063293457031E-14			16	prr7,9 IV	1.726227953933562E-7	1.338379333724221E-7	9.514126586914061E-9
17	Col I	1.254376230701299E-12	4.2797636441570696E-13	3.6176746368408206E-14		28 ED	17	Col I	2.5087524614025976E-7	8.559527288314139E-8	7.235349273681641E-9
18	Col II	1.3728337006387685E-12	2.3422807446418336E-13	2.963250732421875E-14			18	Col II	2.745667401277537E-7	4.684561489283667E-8	5.92650146484375E-9
19	Col III	1.1868255478582284E-12	2.3856136306153646E-13	3.213746643066406E-14			19	Col III	2.3736510957164565E-7	4.771227261230729E-8	6.427493286132813E-9
20	Col IV	1.2534651430514847E-12	2.065731074330734E-13	3.652370452880859E-14			20	Col IV	2.5069302861029693E-7	4.1314621486614676E-8	7.304740905761718E-9
21	prr7,9 I	9.439965759903138E-13	5.626230862462367E-13	5.805496978759765E-14			21	prr7,9 I	1.8879931519806277E-7	1.1252461724924733E-7	1.161099395751953E-8
22	prr7,9 II	7.707518328619666E-13	9.291935744195838E-13	6.462269592285156E-14			22	prr7,9 II	1.541503665723933E-7	1.8583871488391673E-7	1.2924539184570312E-8
23	prr7,9 III	9.687843585315452E-13	6.285705432017378E-13	6.189570617675781E-14			23	prr7,9 III	1.9375687170630903E-7	1.2571410864034754E-7	1.2379141235351562E-8
24	prr7,9 IV	1.16547451074283E-12	2.1048748995718058E-13	3.458097457885742E-14			24	prr7,9 IV	2.3309490214856598E-7	4.209749799143611E-8	6.9161949157714844E-9

Sample analysis order	nmol/gFW	TDP	TMP	THIA
1	Col I	885.3673155522177	99.27745416296075	29.473283456159127			Mean		TDP	TMP	THIA
2	Col II	890.3347328756889	49.80425745724377	19.43391095780131			27 EN	Col	903.1105514312983	58.377214678324826	22.800776756334205
3	Col III	996.6873990985658	58.30624199943764	28.09267761886761			28 ED	Col	766.8519602323545	114.14230855984623	22.100128563367562
4	Col IV	840.0527581987208	26.12090509365715	14.203234992508774			28 EN	Col	708.7355014572627	153.90018407206077	18.87911546319028
5	prr7,9 I	857.8201908553074	82.29339881967259	20.742849614416226			27 EN	prr7,9	960.3561214730375	173.5208146716876	53.59775060445553
6	prr7,9 II	890.3537953284659	199.554733857485	18.71309417286558			28 ED	prr7,9	832.5114124761428	368.51248577692866	38.71740225546809
7	prr7,9 III	1044.9989148966433	138.93647809698714	38.01155021350162			28 EN	prr7,9	726.2764745957589	419.8352113851851	40.32534466004294
8	prr7,9 IV	1048.2515848117334	273.2986479126056	136.92350841703868
9	Col I	817.8618662174064	135.6724672979424	49.39660281496856			STDEV		TDP	TMP	THIA
10	Col II	661.9234162196647	198.24952429821812	18.528518440553643			27 EN	Col	33.180008622075476	15.239203092020402	3.626063867843288
11	Col III	839.8773211426773	50.356332788261696	7.982156901341957			28 ED	Col	40.11419122603355	33.36385488473636	9.351753009264181
12	Col IV	747.7452373496694	72.29090985496272	12.4932360966061			28 EN	Col	13.156048540266871	25.54300793249725	1.2250361762140936
13	prr7,9 I	583.2363259719983	513.9332608252989	44.114164905349966			27 EN	prr7,9	50.252659703550506	40.97956675158741	28.11063756404265
14	prr7,9 II	1041.6966505219289	212.10436744654058	50.22471350795891			28 ED	prr7,9	118.28920963749397	87.31786360652637	5.807422031023975
15	prr7,9 III	1027.4962047419249	222.64260581753507	23.18381564521091			28 EN	prr7,9	92.05967843469897	90.15789119686292	4.134412699440083
16	prr7,9 IV	677.6164686687191	525.36970901834	37.346914963352546
17	Col I	670.7894281825121	228.86436599770408	19.345853672945548			Fold vs Col		TDP	TMP	THIA
18	Col II	716.8844389758591	122.31231042516119	15.473894164082918			27 EN	Col
19	Col III	715.6736972009405	143.85609832856449	19.379376742108956			28 ED	Col
20	Col IV	731.5944414697387	120.56796153681334	21.31733727362371			28 EN	Col
21	prr7,9 I	744.7704741540934	443.88409171300697	45.802737505007975			27 EN	prr7,9	1.0633871124094536	2.9724065395691963	2.3506984510764846
22	prr7,9 II	500.4882031571212	603.3724509218076	41.96278956029323			28 ED	prr7,9	1.0856220700327788	3.2285354171167215	1.7519084626342294
23	prr7,9 III	709.7321307923403	460.4912404408331	45.34483968993242			28 EN	prr7,9	1.0247496747410416	2.7279708202857345	2.135976377636316
24	prr7,9 IV	950.1150902794808	171.59306246509283	28.191011884938113



Sample analysis order		THIA	Extraction volume	Mass
1	Col I	285.237	3.0E-4	0.08509999999999995
2	Col II	121.45	1.9999999999999998E-4	0.05579999999999996
3	Col III	160.685	1.9999999999999998E-4	0.046499999999999986
4	Col IV	150.074	1.9999999999999998E-4	0.06140000000000002
5	prr7,9 I	272.37	1.9999999999999998E-4	0.06780000000000008
6	prr7,9 II	242.808	1.9999999999999998E-4	0.06269999999999998
7	prr7,9 III	164.77	1.9999999999999998E-4	0.05530000000000002				pmol	Thia
8	prr7,9 IV	855.881	3.0E-4	0.0756				6.25E-14	291.3258
9	Col I	203.8	3.0E-4	0.0807000000000001				1.25E-13	464.7634
10	Col II	156.916	3.0E-4	0.0969				2.5E-13	711.93347
11	Col III	135.305	3.0E-4	0.08130000000000004				5.0E-13	964.34528
12	Col IV	162.112	1.9999999999999998E-4	0.0677000000000001				1.0E-12	1763.112
13	prr7,9 I	330.51	3.9999999999999996E-4	0.1059				2.0E-12	4222.5888
14	prr7,9 II	166.392	1.9999999999999998E-4	0.05069999999999997				Slope	1.9906732241935485E15
15	prr7,9 III	288.228	1.9999999999999998E-4	0.05459999999999998				b	256.55
16	prr7,9 IV	273.176	3.9999999999999996E-4	0.10189999999999999
17	Col I	324.4	3.0E-4	0.11220000000000008
18	Col II	187.35	1.9999999999999998E-4	0.07659999999999989
19	Col III	164.641	3.0E-4	0.09950000000000014
20	Col IV	206.925	3.0E-4	0.1027999999999999
21	prr7,9 I	307.41	3.9999999999999996E-4	0.10140000000000005
22	prr7,9 II	445.663	3.9999999999999996E-4	0.12319999999999998
23	prr7,9 III	166.888	3.9999999999999996E-4	0.10920000000000007
24	prr7,9 IV	184.335	3.0E-4	0.0736


Sample analysis order	pmol injected	THIA			Sample analysis order	pmol/µL	THIA
1	Col I	1.4261850000000002E-13		27 EN	1	Col I	1.140948E-8
2	Col II	6.0725E-14			2	Col II	4.8579999999999996E-9
3	Col III	8.03425E-14			3	Col III	6.4274E-9
4	Col IV	7.503700000000001E-14			4	Col IV	6.0029600000000005E-9
5	prr7,9 I	1.36185E-13			5	prr7,9 I	1.0894799999999998E-8
6	prr7,9 II	1.21404E-13			6	prr7,9 II	9.71232E-9
7	prr7,9 III	8.238500000000001E-14			7	prr7,9 III	6.5908E-9
8	prr7,9 IV	4.279405E-13			8	prr7,9 IV	3.423524E-8
9	Col I	1.019E-13		28 EN	9	Col I	8.151999999999999E-9
10	Col II	7.8458E-14			10	Col II	6.276639999999999E-9
11	Col III	6.76525E-14			11	Col III	5.4122E-9
12	Col IV	8.1056E-14			12	Col IV	6.4844799999999995E-9
13	prr7,9 I	1.6525499999999999E-13			13	prr7,9 I	1.3220399999999999E-8
14	prr7,9 II	8.3196E-14			14	prr7,9 II	6.65568E-9
15	prr7,9 III	1.4411400000000002E-13			15	prr7,9 III	1.152912E-8
16	prr7,9 IV	1.3658799999999998E-13			16	prr7,9 IV	1.0927039999999999E-8
17	Col I	1.6219999999999998E-13		28 ED	17	Col I	1.2975999999999998E-8
18	Col II	9.3675E-14			18	Col II	7.493999999999999E-9
19	Col III	8.23205E-14			19	Col III	6.58564E-9
20	Col IV	1.034625E-13			20	Col IV	8.277E-9
21	prr7,9 I	1.5370500000000003E-13			21	prr7,9 I	1.2296400000000002E-8
22	prr7,9 II	2.228315E-13			22	prr7,9 II	1.782652E-8
23	prr7,9 III	8.344400000000001E-14			23	prr7,9 III	6.6755200000000005E-9
24	prr7,9 IV	9.216750000000001E-14			24	prr7,9 IV	7.3734E-9

Sample analysis order	nmol/gFW	THIA
1	Col I	40.22143360752058					THIA
2	Col II	17.412186379928322			27 EN	Col	26.207991701355937
3	Col III	27.644731182795706			28 ED	Col	22.216221609963032
4	Col IV	19.55361563517915			28 EN	Col	24.568159266491413
5	prr7,9 I	32.138053097345086			27 EN	prr7,9	55.70224879793622
6	prr7,9 II	30.980287081339725			28 ED	prr7,9	40.32873922161233
7	prr7,9 III	23.83652802893308			28 EN	prr7,9	40.22297358543073
8	prr7,9 IV	135.854126984127
9	Col I	30.3048327137546					THIA
10	Col II	19.432321981424142			27 EN	Col	5.16468526370469
11	Col III	19.971217712177108			28 ED	Col	2.701505439732336
12	Col IV	19.15651403249628			28 EN	Col	3.534897659186522
13	prr7,9 I	49.935410764872515			27 EN	prr7,9	26.780270173884016
14	prr7,9 II	26.2551479289941			28 ED	prr7,9	5.004580445280961
15	prr7,9 III	42.2312087912088			28 EN	prr7,9	7.812557334225081
16	prr7,9 IV	42.893189401373895
17	Col I	34.695187165775366
18	Col II	19.566579634464773
19	Col III	19.856201005025095
20	Col IV	24.15466926070041
21	prr7,9 I	48.50650887573963
22	prr7,9 II	57.8783116883117
23	prr7,9 III	24.452454212454196
24	prr7,9 IV	30.054619565217386

	Sample analysis order	pmol/µL	TDP	TMP	THIA
27 EN	1	Col I	885.3673155522177	99.27745416296075	29.473283456159127
	2	Col II	890.3347328756889	49.80425745724377	19.43391095780131			TDP	TMP	Thia			TDP	TMP	Thia
	3	Col III	996.6873990985658	58.30624199943764	28.09267761886761	27EN	Col vs prr7.9	0.3784950101359991	0.03887304751915922	0.31894335633099896	27EN	Col vs prr7.9	n.s	*	n.s
	4	Col IV	840.0527581987208	26.12090509365715	14.203234992508774	28ED	Col vs prr7.9	0.6179695046170415	0.034584532576066675	0.18190213222393758	28ED	Col vs prr7.9	n.s	*	n.s
	5	prr7,9 I	857.8201908553074	82.29339881967259	20.742849614416226	28EN	Col vs prr7.9	0.856606669838744	0.029646233508172554	0.002517983320163621	28EN	Col vs prr7.9	n.s	*	**
	6	prr7,9 II	890.3537953284659	199.554733857485	18.71309417286558	Col	27 EN vs 28 EN	0.0397230310306063	0.17923948406921422	0.9465796126527969	Col	27 EN vs 28 EN	*	n.s	n.s
	7	prr7,9 III	1044.9989148966433	138.93647809698714	38.01155021350162	prr7.9	27 EN vs 28 EN	0.3582725251207178	0.08970590900428527	0.6227325689414196	prr7.9	27 EN vs 28 EN	n.s	n.s	n.s
	8	prr7,9 IV	1048.2515848117334	273.2986479126056	136.92350841703868	Col	28 EN vs 28 ED	0.21778917569917583	0.3805817643529822	0.7443616614354294	Col	28 EN vs 28 ED	n.s	n.s	n.s
28 EN	9	Col I	817.8618662174064	135.6724672979424	49.39660281496856	prr7.9	28 EN vs 28 ED	0.5050727905942308	0.6967992204231616	0.8290329831982349	prr7.9	28 EN vs 28 ED	n.s	n.s	n.s
	10	Col II	661.9234162196647	198.24952429821812	18.528518440553643	Col	27 EN vs 28 ED	0.0015937499093712326	0.018331131421305904	0.3450625364371603	Col	27 EN vs 28 ED	**	*	n.s
	11	Col III	839.8773211426773	50.356332788261696	7.982156901341957	prr7.9	27 EN vs 28 ED	0.06709678940629217	0.047343595565421566	0.6568931221269751	prr7.10	27 EN vs 28 ED	n.s	*	n.s
	12	Col IV	747.7452373496694	72.29090985496272	12.4932360966061
	13	prr7,9 I	583.2363259719983	513.9332608252989	44.114164905349966
	14	prr7,9 II	1041.6966505219289	212.10436744654058	50.22471350795891
	15	prr7,9 III	1027.4962047419249	222.64260581753507	23.18381564521091
	16	prr7,9 IV	677.6164686687191	525.36970901834	37.346914963352546
28 ED	17	Col I	670.7894281825121	228.86436599770408	19.345853672945548
	18	Col II	716.8844389758591	122.31231042516119	15.473894164082918
	19	Col III	715.6736972009405	143.85609832856449	19.379376742108956
	20	Col IV	731.5944414697387	120.56796153681334	21.31733727362371
	21	prr7,9 I	744.7704741540934	443.88409171300697	45.802737505007975
	22	prr7,9 II	500.4882031571212	603.3724509218076	41.96278956029323
	23	prr7,9 III	709.7321307923403	460.4912404408331	45.34483968993242
	24	prr7,9 IV	950.1150902794808	171.59306246509283	28.191011884938113

	Sample analysis order	pmol/µL	THIA
27 EN	1	Col I	100.55358401880146
	2	Col II	43.530465949820815			Thia			Thia
	3	Col III	69.11182795698926	27EN	Col vs prr7.9	0.3210491931812907	27EN	Col vs prr7.9	n.s
	4	Col IV	48.884039087947876	28ED	Col vs prr7.9	0.018960465831258648	28ED	Col vs prr7.9	*
	5	prr7,9 I	80.34513274336271	28EN	Col vs prr7.9	0.11769982614260949	28EN	Col vs prr7.9	n.s
	6	prr7,9 II	77.4507177033493	Col	27 EN vs 28 EN	0.5189961799445153	Col	27 EN vs 28 EN	n.s
	7	prr7,9 III	59.5913200723327	prr7.9	27 EN vs 28 EN	0.5930067610455964	prr7.9	27 EN vs 28 EN	n.s
	8	prr7,9 IV	339.6353174603173	Col	28 EN vs 28 ED	0.6160276879003956	Col	28 EN vs 28 ED	n.s
28 EN	9	Col I	75.76208178438651	prr7.9	28 EN vs 28 ED	0.9912742373838004	prr7.9	28 EN vs 28 ED	n.s
	10	Col II	48.58080495356035	Col	27 EN vs 28 ED	0.802073365028804	Col	27 EN vs 28 ED	n.s
	11	Col III	49.92804428044277	prr7.9	27 EN vs 28 ED	0.5990358884307454	prr7.10	27 EN vs 28 ED	n.s
	12	Col IV	47.89128508124068
	13	prr7,9 I	124.83852691218128
	14	prr7,9 II	65.63786982248523
	15	prr7,9 III	105.578021978022
	16	prr7,9 IV	107.23297350343472
28 ED	17	Col I	86.73796791443841
	18	Col II	48.91644908616194
	19	Col III	49.640502512562726
	20	Col IV	60.386673151751026
	21	prr7,9 I	121.26627218934907
	22	prr7,9 II	144.6957792207792
	23	prr7,9 III	61.13113553113548
	24	prr7,9 IV	75.13654891304348

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3600

	39.5611464977265		41.9899046421051
	39.5390212535858		41.9537961483002
	39.5255208015442		41.9229626655579
	39.520388841629		41.89772605896
	39.5224452018738		41.8781757354737
	39.5299077033997		41.8645560741425
	39.5410418510437		41.8574094772339
	39.5545423030853		41.8573915958405
	39.5693421363831		41.8648481369019
	39.5842432975769		41.8794214725495
	39.5978331565857		41.900110244751
	39.6086931228638		41.9254302978516
	39.6155774593353		41.9534206390381
	39.6173119544983		41.9816434383393
	39.6123707294464		42.0073628425598
	39.5988643169403		42.0279383659363
	39.5750284194946		42.041552066803
	39.5399153232575		42.0476019382477
	39.493727684021		42.0464634895325
	39.4376993179321		42.0389413833618
	39.3737316131592		42.025762796402
	39.3042325973511		42.0073747634888
	39.2319619655609		41.9840753078461
	39.1597151756287		41.9559955596924
	39.0898823738098		41.923052072525
	39.0240550041199		41.8849050998688
	38.9629900455475		41.8412506580353
	38.9069318771363		41.7922198772431
	38.8558506965637		41.7385518550873
	38.8095915317536		41.6814088821411
	38.7680053710938		41.6219830513001
	38.7308895587921		41.5612101554871
	38.6979758739472		41.4996981620789
	38.6687815189362		41.4379298686981
	38.642430305481		41.3763999938965
	38.6175811290741		41.3156509399414
	38.5928094387055		41.2563741207123
	38.5669052600861		41.1994397640228
	38.539332151413		41.1458790302277
	38.5105609893799		41.0967886447907
	38.4820461273194		41.0530149936676
	38.4561955928803		41.0148620605469
	38.4362280368805		40.9820377826691
	38.4257853031159		40.9540057182312
	38.42813372612		40.9304559230805
	38.4454846382141		40.9116744995117
	38.4783267974854		40.8986151218415
	38.5252594947815		40.8927142620087
	38.5831713676453		40.8956170082092
	38.6475503444672		40.9089148044586
	38.7127876281738		40.9336805343628
	38.7727737426758		40.96999168396
	38.8215184211731		41.0163521766663
	38.8538122177124		41.0695612430573
	38.8658225536347		41.1248981952667
	38.8553202152252		41.1768615245819
	38.821691274643		41.2199139595032
	38.7658059597016		41.2488698959351
	38.6898756027222		41.2591338157654
	38.5971307754517		41.2468671798706
	38.4915053844452		41.2093758583069
	38.3771181106568		41.1455631256104
	38.2578015327454		41.0560607910156
	38.1369650363922		40.9428536891937
	38.0175173282624		40.8089876174927
	37.9018127918244		40.6584978103638
	37.7913653850556		40.4964864253998
	37.6867353916168		40.3288364410401
	37.5878095626831		40.1615500450134
	37.4943196773529		39.9999439716339
	37.4061763286591		39.8481965065003
	37.3233914375305		39.7092044353485
	37.2458815574646		39.5843148231507
	37.1735155582428		39.4731521606446
	37.1064066886902		39.3738269805908
	37.0448768138886		39.2837047576905
	36.9890213012696		39.2002761363983
	36.9380354881287		39.1216099262238
	36.8899822235108		39.0462458133698
	36.8420958518982		38.9731287956238
	36.7913544178009		38.9018654823303
	36.7349982261658		38.832825422287
	36.6708278656006		38.7667536735535
	36.597490310669		38.7039601802826
	36.5147650241852		38.6437892913819
	36.4237487316132		38.5848104953766
	36.3267302513123		38.5253429412842
	36.226898431778		38.4637773036957
	36.1276686191559		38.3985698223114
	36.0320031642914		38.3282661437988
	35.9419286251068		38.2518112659455
	35.8584403991699		38.1690084934235
	35.7816874980927		38.0806088447571
	35.7112884521485		37.9879474639893
	35.6463432312012		37.8924727439881
	35.5856001377106		37.7954602241516
	35.5277001857758		37.6979887485504
	35.4714870452881		37.6010239124298
	35.4162275791168		37.5053465366364
	35.361522436142		37.4114036560059