Testing thiamine metabolites in Col and prr7prr9

Selected Cell

Cell:

Value:

Read.Me Sheet1 Results-std curve Repeat for thiamin Graph-stats Graph-stats (2) Chromatogram

Testing thiamine vitamers in plant samples from study L&H3
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites

Analysis Protocol	From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight.

Sheets of this workbook	Contents
Sheet1	Raw data and sampling plan
Results-std curve	Data for individual replicates and standard curve
Repeat for thiamin
Graph-stats	Summary statistics, means, t-test statistics except thiamine, and final charts
Graph-stats (2)	Summary statistics, means, t-test statistics only for thiamine
Chromatogram	raw data traces

Credit
Experimental design, plant growth and sampling	Yin Hoon Chew, Andrew Millar group University of Edinburgh
Analytical design, execution and data analysis	Michael Moulin, Teresa Fitzpatrick group University of Geneva
File Curation	Andrew Millar




numbering	Col				prr7,prr9					replicat	Col				prr7,prr9
27 EN	1	2	3	4	5	6	7	8		27 EN	1	2	3	4	1	2	3	4
28 ED	9	10	11	12	13	14	15	16		28 ED	1	2	3	4	1	2	3	4
28 EN	17	18	19	20	21	22	23	24		28 EN	1	2	3	4	1	2	3	4

mass tube	Col				prr7,prr9
27 EN	0.9438	0.9547	0.9455	0.9373	0.9437	0.9561	0.9486	0.9465
28 ED	0.9427	0.9391	0.9409	0.9434	0.9522	0.9437	0.9438	0.9442
28 EN	0.944	0.9421	0.9439	0.9465	0.9562	0.9436	0.9506	0.9442

mass tube+sample	Col				prr7,prr9
27 EN	1.0289	1.0105	0.992	0.9987	1.0115	1.0188	1.0039	1.0221
28 ED	1.0234	1.036	1.0222	1.0111	1.0581	0.9944	0.9984	1.0461
28 EN	1.0562	1.0187	1.0434	1.0493	1.0576	1.0668	1.0598	1.0178

mass sample	Col				prr7,prr9
27 EN	0.08509999999999995	0.05579999999999996	0.046499999999999986	0.06140000000000001	0.06780000000000008	0.06269999999999998	0.055300000000000016	0.0756
28 ED	0.0807000000000001	0.09689999999999999	0.08130000000000004	0.0677000000000001	0.1059	0.05069999999999997	0.05459999999999998	0.10189999999999999
28 EN	0.11220000000000008	0.07659999999999989	0.09950000000000014	0.10279999999999989	0.10140000000000005	0.12319999999999998	0.10920000000000007	0.0736

mass sample	Col				prr7,prr9
27 EN	300	200	200	200	200	200	200	300
28 ED	300	300	300	200	400	200	200	400
28 EN	300	300	300	300	40	400	400	300


	Extraxtion volume	Mass			Sample analysis order		TDP	TMP	THIA	Extraction volume	Mass
1	300	85.09999999999995			0.03125	pmol	98.93505859375	100.81101989746094	217.923583984375
2	200	55.79999999999996			0.0625	pmol	252.35	237.66	289.42
3	200	46.499999999999986			0.125	pmol	309.66	318.61	576.96
4	200	61.40000000000001			0.25	pmol	502.94	515.39	955.81
5	200	67.80000000000008			0.5	pmol	850.95	859.87	1269.7
6	200	62.699999999999974			1	pmol	1621.6	1673.7	2243.1
7	200	55.30000000000002			2	pmol	3490.9	3645	4747
8	300	75.6			4	pmol	6814.1	7077	9161
9	300	80.7000000000001			1	Col I	2180.39501953125	301.9862060546875	83.60588073730469	300	85.09999999999995
10	300	96.89999999999999			9	Col I	1917.8831787109375	375.14276123046875	132.87686157226562	300	80.7000000000001
11	300	81.30000000000004			17	Col I	2178.0859375	806.1314697265625	72.3534927368164	300	112.20000000000007
12	200	67.70000000000009			5	prr7,9 I	2514.615478515625	299.6667175292969	70.3182601928711	200	67.80000000000008
13	400	105.89999999999999			13	prr7,9 I	1365.0078125	1249.138671875	116.79225158691406	400	105.89999999999999
14	200	50.69999999999997			21	prr7,9 I	1654.855712890625	1042.51416015625	116.10993957519531	400	101.40000000000005
15	200	54.59999999999998			Standard	1	1645.3131103515625	1719.94091796875	2256.296630859375
16	400	101.89999999999999			2	Col II	2157.2509765625	176.75811767578125	54.220611572265625	200	55.79999999999996
17	300	112.20000000000007			10	Col II	1865.593994140625	616.8743286132812	59.84711456298828	300	96.89999999999999
18	200	76.5999999999999			18	Col II	2377.778564453125	465.9914245605469	59.2650146484375	200	76.5999999999999
19	300	99.50000000000014			6	prr7,9 II	2416.207275390625	603.93408203125	58.665550231933594	200	62.699999999999974
20	300	102.7999999999999			14	prr7,9 II	2289.3037109375	526.7593383789062	127.31964874267578	200	50.69999999999997
21	400	101.40000000000005			22	prr7,9 II	1362.8040771484375	1686.056884765625	129.24539184570312	400	123.19999999999997
22	400	123.19999999999997			Standard	0.5	808.2450561523438	831.448486328125	1122.8255615234375
23	400	109.20000000000007			3	Col III	2016.7115478515625	173.78077697753906	65.31547546386719	200	46.499999999999986
24	300	73.6			11	Col III	1981.958740234375	174.57398986816406	21.63164520263672	300	81.30000000000004
					19	Col III	2064.210693359375	473.5988464355469	64.27493286132812	300	99.50000000000014
					7	prr7,9 III	2498.947509765625	391.9966735839844	105.10193634033203	200	55.30000000000002
	Derivatization				15	prr7,9 III	2427.84375	588.3184204101562	63.29181671142578	200	54.59999999999998
	25 µL				23	prr7,9 III	1696.642333984375	1158.29	123.79141235351562	400	109.20000000000007
	injection	injection			Standard	1	1606.5908203125	1679.7308349609375	2153.149169921875
	20 µL				4	Col IV	2237.2646484375	125.17689514160156	43.60393142700195	200	61.40000000000001
					12	Col IV	2196.937255859375	269.585	42.28960418701172	200	67.70000000000009
					20	Col IV	2176.550048828125	417.441	73.04740905761719	300	102.7999999999999
					8	prr7,9 IV	2290.056396484375	659.33	345.0472412109375	300	75.6
					16	prr7,9 IV	1518.505859375	1229.6	95.14126586914062	400	101.89999999999999
					24	prr7,9 IV	2028.2176513671875	424.313	69.16194915771484	300	73.6
					Standard	0.25	447.85986328125	458.4064636230469	788.3421020507812
					Standard	0.03125	98.93505859375	100.81101989746094	217.923583984375



Sample analysis order		TDP	TMP	THIA	Extraction volume	Mass
1	Col I	2180.39501953125	301.9862060546875	83.60588073730469	3.0E-4	0.08509999999999995
2	Col II	2157.2509765625	176.75811767578125	54.220611572265625	1.9999999999999998E-4	0.05579999999999996
3	Col III	2016.7115478515625	173.78077697753906	65.31547546386719	1.9999999999999998E-4	0.046499999999999986
4	Col IV	2237.2646484375	125.17689514160156	43.60393142700195	1.9999999999999998E-4	0.06140000000000002
5	prr7,9 I	2514.615478515625	299.6667175292969	70.3182601928711	1.9999999999999998E-4	0.06780000000000008
6	prr7,9 II	2416.207275390625	603.93408203125	58.665550231933594	1.9999999999999998E-4	0.06269999999999998
7	prr7,9 III	2498.947509765625	391.9966735839844	105.10193634033203	1.9999999999999998E-4	0.05530000000000002				pmol	TDP	TMP	Thia
8	prr7,9 IV	2290.056396484375	659.33	345.0472412109375	3.0E-4	0.0756				3.125E-14	98.93505859375	100.81101989746094	217.923583984375
9	Col I	1917.8831787109375	375.14276123046875	132.87686157226562	3.0E-4	0.0807000000000001				6.25E-14	252.35	237.66	289.42
10	Col II	1865.593994140625	616.8743286132812	59.84711456298828	3.0E-4	0.0969				1.25E-13	309.66	318.61	576.96
11	Col III	1981.958740234375	174.57398986816406	21.63164520263672	3.0E-4	0.08130000000000004				2.5E-13	502.94	515.39	955.81
12	Col IV	2196.937255859375	269.585	42.28960418701172	1.9999999999999998E-4	0.0677000000000001				5.0E-13	850.95	859.87	1269.7
13	prr7,9 I	1365.0078125	1249.138671875	116.79225158691406	3.9999999999999996E-4	0.1059				1.0E-12	1621.6	1673.7	2243.1
14	prr7,9 II	2289.3037109375	526.7593383789062	127.31964874267578	1.9999999999999998E-4	0.05069999999999997				2.0E-12	3490.9	3645	4747
15	prr7,9 III	2427.84375	588.3184204101562	63.29181671142578	1.9999999999999998E-4	0.05459999999999998				4.0E-12	6814.1	7077	9161
16	prr7,9 IV	1518.505859375	1229.6	95.14126586914062	3.9999999999999996E-4	0.10189999999999999				Slope	1.685774878178111E15	1.7555770182597208E15	2.2371156417932975E15
17	Col I	2178.0859375	806.1314697265625	72.3534927368164	3.0E-4	0.11220000000000008				b	63.49	54.786	204.24
18	Col II	2377.778564453125	465.9914245605469	59.2650146484375	1.9999999999999998E-4	0.07659999999999989
19	Col III	2064.210693359375	473.5988464355469	64.27493286132812	3.0E-4	0.09950000000000014
20	Col IV	2176.550048828125	417.441	73.04740905761719	3.0E-4	0.1027999999999999
21	prr7,9 I	1654.855712890625	1042.51416015625	116.10993957519531	3.9999999999999996E-4	0.10140000000000005
22	prr7,9 II	1362.8040771484375	1686.056884765625	129.24539184570312	3.9999999999999996E-4	0.12319999999999998
23	prr7,9 III	1696.642333984375	1158.29	123.79141235351562	3.9999999999999996E-4	0.10920000000000007
24	prr7,9 IV	2028.2176513671875	424.313	69.16194915771484	3.0E-4	0.0736


Sample analysis order	pmol injected	TDP	TMP	THIA			Sample analysis order	pmol/µL	TDP	TMP	THIA
1	Col I	1.2557459758915614E-12	1.4080852248779928E-13	4.180294036865234E-14		27 EN	1	Col I	2.511491951783123E-7	2.8161704497559855E-8	8.360588073730468E-9
2	Col II	1.2420169523615854E-12	6.947693915285502E-14	2.7110305786132812E-14			2	Col II	2.4840339047231705E-7	1.3895387830571003E-8	5.422061157226562E-9
3	Col III	1.1586491014520826E-12	6.778100632434624E-14	3.265773773193359E-14			3	Col III	2.3172982029041653E-7	1.3556201264869248E-8	6.531547546386718E-9
4	Col IV	1.289480983835037E-12	4.009558931876374E-14	2.1801965713500978E-14			4	Col IV	2.5789619676700736E-7	8.019117863752749E-9	4.360393142700195E-9
5	prr7,9 I	1.4540052234997483E-12	1.3948731099934524E-13	3.515913009643555E-14			5	prr7,9 I	2.908010446999496E-7	2.7897462199869047E-8	7.0318260192871094E-9
6	prr7,9 II	1.3956295741773702E-12	3.1280204532160763E-13	2.93327751159668E-14			6	prr7,9 II	2.79125914835474E-7	6.256040906432153E-8	5.8665550231933595E-9
7	prr7,9 III	1.4447109998446106E-12	1.9207968096908483E-13	5.2550968170166016E-14			7	prr7,9 III	2.889421999689221E-7	3.8415936193816965E-8	1.0510193634033203E-8
8	prr7,9 IV	1.3207969968627843E-12	3.443562963698831E-13	1.7252362060546875E-13			8	prr7,9 IV	2.6415939937255685E-7	6.887125927397661E-8	3.450472412109375E-8
9	Col I	1.1000242100624132E-12	1.824794685157328E-13	6.643843078613281E-14		28 EN	9	Col I	2.2000484201248262E-7	3.6495893703146554E-8	1.328768615722656E-8
10	Col II	1.0690063171947585E-12	3.2017298174162225E-13	2.992355728149414E-14			10	Col II	2.138012634389517E-7	6.403459634832445E-8	5.9847114562988275E-9
11	Col III	1.1380337701483285E-12	6.823283092809465E-14	1.081582260131836E-14			11	Col III	2.2760675402966568E-7	1.364656618561893E-8	2.1631645202636718E-9
12	Col IV	1.2655588142143173E-12	1.2235236492952458E-13	2.114480209350586E-14			12	Col IV	2.5311176284286343E-7	2.4470472985904917E-8	4.228960418701171E-9
13	prr7,9 I	7.72059086505433E-13	6.803191540174895E-13	5.839612579345703E-14			13	prr7,9 I	1.5441181730108656E-7	1.360638308034979E-7	1.1679225158691405E-8
14	prr7,9 II	1.3203505045365442E-12	2.6884228573849006E-13	6.365982437133789E-14			14	prr7,9 II	2.640701009073088E-7	5.376845714769801E-8	1.2731964874267577E-8
15	prr7,9 III	1.4025323194727273E-12	3.039071569409353E-13	3.164590835571289E-14			15	prr7,9 III	2.8050646389454546E-7	6.078143138818706E-8	6.329181671142578E-9
16	prr7,9 IV	8.63113976966781E-13	6.691896668621105E-13	4.757063293457031E-14			16	prr7,9 IV	1.726227953933562E-7	1.338379333724221E-7	9.514126586914061E-9
17	Col I	1.254376230701299E-12	4.2797636441570696E-13	3.6176746368408206E-14		28 ED	17	Col I	2.5087524614025976E-7	8.559527288314139E-8	7.235349273681641E-9
18	Col II	1.3728337006387685E-12	2.3422807446418336E-13	2.963250732421875E-14			18	Col II	2.745667401277537E-7	4.684561489283667E-8	5.92650146484375E-9
19	Col III	1.1868255478582284E-12	2.3856136306153646E-13	3.213746643066406E-14			19	Col III	2.3736510957164565E-7	4.771227261230729E-8	6.427493286132813E-9
20	Col IV	1.2534651430514847E-12	2.065731074330734E-13	3.652370452880859E-14			20	Col IV	2.5069302861029693E-7	4.1314621486614676E-8	7.304740905761718E-9
21	prr7,9 I	9.439965759903138E-13	5.626230862462367E-13	5.805496978759765E-14			21	prr7,9 I	1.8879931519806277E-7	1.1252461724924733E-7	1.161099395751953E-8
22	prr7,9 II	7.707518328619666E-13	9.291935744195838E-13	6.462269592285156E-14			22	prr7,9 II	1.541503665723933E-7	1.8583871488391673E-7	1.2924539184570312E-8
23	prr7,9 III	9.687843585315452E-13	6.285705432017378E-13	6.189570617675781E-14			23	prr7,9 III	1.9375687170630903E-7	1.2571410864034754E-7	1.2379141235351562E-8
24	prr7,9 IV	1.16547451074283E-12	2.1048748995718058E-13	3.458097457885742E-14			24	prr7,9 IV	2.3309490214856598E-7	4.209749799143611E-8	6.9161949157714844E-9

Sample analysis order	nmol/gFW	TDP	TMP	THIA
1	Col I	885.3673155522177	99.27745416296075	29.473283456159127			Mean		TDP	TMP	THIA
2	Col II	890.3347328756889	49.80425745724377	19.43391095780131			27 EN	Col	903.1105514312983	58.377214678324826	22.800776756334205
3	Col III	996.6873990985658	58.30624199943764	28.09267761886761			28 ED	Col	766.8519602323545	114.14230855984623	22.100128563367562
4	Col IV	840.0527581987208	26.12090509365715	14.203234992508774			28 EN	Col	708.7355014572627	153.90018407206077	18.87911546319028
5	prr7,9 I	857.8201908553074	82.29339881967259	20.742849614416226			27 EN	prr7,9	960.3561214730375	173.5208146716876	53.59775060445553
6	prr7,9 II	890.3537953284659	199.554733857485	18.71309417286558			28 ED	prr7,9	832.5114124761428	368.51248577692866	38.71740225546809
7	prr7,9 III	1044.9989148966433	138.93647809698714	38.01155021350162			28 EN	prr7,9	726.2764745957589	419.8352113851851	40.32534466004294
8	prr7,9 IV	1048.2515848117334	273.2986479126056	136.92350841703868
9	Col I	817.8618662174064	135.6724672979424	49.39660281496856			STDEV		TDP	TMP	THIA
10	Col II	661.9234162196647	198.24952429821812	18.528518440553643			27 EN	Col	33.180008622075476	15.239203092020402	3.626063867843288
11	Col III	839.8773211426773	50.356332788261696	7.982156901341957			28 ED	Col	40.11419122603355	33.36385488473636	9.351753009264181
12	Col IV	747.7452373496694	72.29090985496272	12.4932360966061			28 EN	Col	13.156048540266871	25.54300793249725	1.2250361762140936
13	prr7,9 I	583.2363259719983	513.9332608252989	44.114164905349966			27 EN	prr7,9	50.252659703550506	40.97956675158741	28.11063756404265
14	prr7,9 II	1041.6966505219289	212.10436744654058	50.22471350795891			28 ED	prr7,9	118.28920963749397	87.31786360652637	5.807422031023975
15	prr7,9 III	1027.4962047419249	222.64260581753507	23.18381564521091			28 EN	prr7,9	92.05967843469897	90.15789119686292	4.134412699440083
16	prr7,9 IV	677.6164686687191	525.36970901834	37.346914963352546
17	Col I	670.7894281825121	228.86436599770408	19.345853672945548			Fold vs Col		TDP	TMP	THIA
18	Col II	716.8844389758591	122.31231042516119	15.473894164082918			27 EN	Col
19	Col III	715.6736972009405	143.85609832856449	19.379376742108956			28 ED	Col
20	Col IV	731.5944414697387	120.56796153681334	21.31733727362371			28 EN	Col
21	prr7,9 I	744.7704741540934	443.88409171300697	45.802737505007975			27 EN	prr7,9	1.0633871124094536	2.9724065395691963	2.3506984510764846
22	prr7,9 II	500.4882031571212	603.3724509218076	41.96278956029323			28 ED	prr7,9	1.0856220700327788	3.2285354171167215	1.7519084626342294
23	prr7,9 III	709.7321307923403	460.4912404408331	45.34483968993242			28 EN	prr7,9	1.0247496747410416	2.7279708202857345	2.135976377636316
24	prr7,9 IV	950.1150902794808	171.59306246509283	28.191011884938113



Sample analysis order		THIA	Extraction volume	Mass
1	Col I	285.237	3.0E-4	0.08509999999999995
2	Col II	121.45	1.9999999999999998E-4	0.05579999999999996
3	Col III	160.685	1.9999999999999998E-4	0.046499999999999986
4	Col IV	150.074	1.9999999999999998E-4	0.06140000000000002
5	prr7,9 I	272.37	1.9999999999999998E-4	0.06780000000000008
6	prr7,9 II	242.808	1.9999999999999998E-4	0.06269999999999998
7	prr7,9 III	164.77	1.9999999999999998E-4	0.05530000000000002				pmol	Thia
8	prr7,9 IV	855.881	3.0E-4	0.0756				6.25E-14	291.3258
9	Col I	203.8	3.0E-4	0.0807000000000001				1.25E-13	464.7634
10	Col II	156.916	3.0E-4	0.0969				2.5E-13	711.93347
11	Col III	135.305	3.0E-4	0.08130000000000004				5.0E-13	964.34528
12	Col IV	162.112	1.9999999999999998E-4	0.0677000000000001				1.0E-12	1763.112
13	prr7,9 I	330.51	3.9999999999999996E-4	0.1059				2.0E-12	4222.5888
14	prr7,9 II	166.392	1.9999999999999998E-4	0.05069999999999997				Slope	1.9906732241935485E15
15	prr7,9 III	288.228	1.9999999999999998E-4	0.05459999999999998				b	256.55
16	prr7,9 IV	273.176	3.9999999999999996E-4	0.10189999999999999
17	Col I	324.4	3.0E-4	0.11220000000000008
18	Col II	187.35	1.9999999999999998E-4	0.07659999999999989
19	Col III	164.641	3.0E-4	0.09950000000000014
20	Col IV	206.925	3.0E-4	0.1027999999999999
21	prr7,9 I	307.41	3.9999999999999996E-4	0.10140000000000005
22	prr7,9 II	445.663	3.9999999999999996E-4	0.12319999999999998
23	prr7,9 III	166.888	3.9999999999999996E-4	0.10920000000000007
24	prr7,9 IV	184.335	3.0E-4	0.0736


Sample analysis order	pmol injected	THIA			Sample analysis order	pmol/µL	THIA
1	Col I	1.4261850000000002E-13		27 EN	1	Col I	1.140948E-8
2	Col II	6.0725E-14			2	Col II	4.8579999999999996E-9
3	Col III	8.03425E-14			3	Col III	6.4274E-9
4	Col IV	7.503700000000001E-14			4	Col IV	6.0029600000000005E-9
5	prr7,9 I	1.36185E-13			5	prr7,9 I	1.0894799999999998E-8
6	prr7,9 II	1.21404E-13			6	prr7,9 II	9.71232E-9
7	prr7,9 III	8.238500000000001E-14			7	prr7,9 III	6.5908E-9
8	prr7,9 IV	4.279405E-13			8	prr7,9 IV	3.423524E-8
9	Col I	1.019E-13		28 EN	9	Col I	8.151999999999999E-9
10	Col II	7.8458E-14			10	Col II	6.276639999999999E-9
11	Col III	6.76525E-14			11	Col III	5.4122E-9
12	Col IV	8.1056E-14			12	Col IV	6.4844799999999995E-9
13	prr7,9 I	1.6525499999999999E-13			13	prr7,9 I	1.3220399999999999E-8
14	prr7,9 II	8.3196E-14			14	prr7,9 II	6.65568E-9
15	prr7,9 III	1.4411400000000002E-13			15	prr7,9 III	1.152912E-8
16	prr7,9 IV	1.3658799999999998E-13			16	prr7,9 IV	1.0927039999999999E-8
17	Col I	1.6219999999999998E-13		28 ED	17	Col I	1.2975999999999998E-8
18	Col II	9.3675E-14			18	Col II	7.493999999999999E-9
19	Col III	8.23205E-14			19	Col III	6.58564E-9
20	Col IV	1.034625E-13			20	Col IV	8.277E-9
21	prr7,9 I	1.5370500000000003E-13			21	prr7,9 I	1.2296400000000002E-8
22	prr7,9 II	2.228315E-13			22	prr7,9 II	1.782652E-8
23	prr7,9 III	8.344400000000001E-14			23	prr7,9 III	6.6755200000000005E-9
24	prr7,9 IV	9.216750000000001E-14			24	prr7,9 IV	7.3734E-9

Sample analysis order	nmol/gFW	THIA
1	Col I	40.22143360752058					THIA
2	Col II	17.412186379928322			27 EN	Col	26.207991701355937
3	Col III	27.644731182795706			28 ED	Col	22.216221609963032
4	Col IV	19.55361563517915			28 EN	Col	24.568159266491413
5	prr7,9 I	32.138053097345086			27 EN	prr7,9	55.70224879793622
6	prr7,9 II	30.980287081339725			28 ED	prr7,9	40.32873922161233
7	prr7,9 III	23.83652802893308			28 EN	prr7,9	40.22297358543073
8	prr7,9 IV	135.854126984127
9	Col I	30.3048327137546					THIA
10	Col II	19.432321981424142			27 EN	Col	5.16468526370469
11	Col III	19.971217712177108			28 ED	Col	2.701505439732336
12	Col IV	19.15651403249628			28 EN	Col	3.534897659186522
13	prr7,9 I	49.935410764872515			27 EN	prr7,9	26.780270173884016
14	prr7,9 II	26.2551479289941			28 ED	prr7,9	5.004580445280961
15	prr7,9 III	42.2312087912088			28 EN	prr7,9	7.812557334225081
16	prr7,9 IV	42.893189401373895
17	Col I	34.695187165775366
18	Col II	19.566579634464773
19	Col III	19.856201005025095
20	Col IV	24.15466926070041
21	prr7,9 I	48.50650887573963
22	prr7,9 II	57.8783116883117
23	prr7,9 III	24.452454212454196
24	prr7,9 IV	30.054619565217386

	Sample analysis order	pmol/µL	TDP	TMP	THIA
27 EN	1	Col I	885.3673155522177	99.27745416296075	29.473283456159127
	2	Col II	890.3347328756889	49.80425745724377	19.43391095780131			TDP	TMP	Thia			TDP	TMP	Thia
	3	Col III	996.6873990985658	58.30624199943764	28.09267761886761	27EN	Col vs prr7.9	0.3784950101359991	0.03887304751915922	0.31894335633099896	27EN	Col vs prr7.9	n.s	*	n.s
	4	Col IV	840.0527581987208	26.12090509365715	14.203234992508774	28ED	Col vs prr7.9	0.6179695046170415	0.034584532576066675	0.18190213222393758	28ED	Col vs prr7.9	n.s	*	n.s
	5	prr7,9 I	857.8201908553074	82.29339881967259	20.742849614416226	28EN	Col vs prr7.9	0.856606669838744	0.029646233508172554	0.002517983320163621	28EN	Col vs prr7.9	n.s	*	**
	6	prr7,9 II	890.3537953284659	199.554733857485	18.71309417286558	Col	27 EN vs 28 EN	0.0397230310306063	0.17923948406921422	0.9465796126527969	Col	27 EN vs 28 EN	*	n.s	n.s
	7	prr7,9 III	1044.9989148966433	138.93647809698714	38.01155021350162	prr7.9	27 EN vs 28 EN	0.3582725251207178	0.08970590900428527	0.6227325689414196	prr7.9	27 EN vs 28 EN	n.s	n.s	n.s
	8	prr7,9 IV	1048.2515848117334	273.2986479126056	136.92350841703868	Col	28 EN vs 28 ED	0.21778917569917583	0.3805817643529822	0.7443616614354294	Col	28 EN vs 28 ED	n.s	n.s	n.s
28 EN	9	Col I	817.8618662174064	135.6724672979424	49.39660281496856	prr7.9	28 EN vs 28 ED	0.5050727905942308	0.6967992204231616	0.8290329831982349	prr7.9	28 EN vs 28 ED	n.s	n.s	n.s
	10	Col II	661.9234162196647	198.24952429821812	18.528518440553643	Col	27 EN vs 28 ED	0.0015937499093712326	0.018331131421305904	0.3450625364371603	Col	27 EN vs 28 ED	**	*	n.s
	11	Col III	839.8773211426773	50.356332788261696	7.982156901341957	prr7.9	27 EN vs 28 ED	0.06709678940629217	0.047343595565421566	0.6568931221269751	prr7.10	27 EN vs 28 ED	n.s	*	n.s
	12	Col IV	747.7452373496694	72.29090985496272	12.4932360966061
	13	prr7,9 I	583.2363259719983	513.9332608252989	44.114164905349966
	14	prr7,9 II	1041.6966505219289	212.10436744654058	50.22471350795891
	15	prr7,9 III	1027.4962047419249	222.64260581753507	23.18381564521091
	16	prr7,9 IV	677.6164686687191	525.36970901834	37.346914963352546
28 ED	17	Col I	670.7894281825121	228.86436599770408	19.345853672945548
	18	Col II	716.8844389758591	122.31231042516119	15.473894164082918
	19	Col III	715.6736972009405	143.85609832856449	19.379376742108956
	20	Col IV	731.5944414697387	120.56796153681334	21.31733727362371
	21	prr7,9 I	744.7704741540934	443.88409171300697	45.802737505007975
	22	prr7,9 II	500.4882031571212	603.3724509218076	41.96278956029323
	23	prr7,9 III	709.7321307923403	460.4912404408331	45.34483968993242
	24	prr7,9 IV	950.1150902794808	171.59306246509283	28.191011884938113

	Sample analysis order	pmol/µL	THIA
27 EN	1	Col I	100.55358401880146
	2	Col II	43.530465949820815			Thia			Thia
	3	Col III	69.11182795698926	27EN	Col vs prr7.9	0.3210491931812907	27EN	Col vs prr7.9	n.s
	4	Col IV	48.884039087947876	28ED	Col vs prr7.9	0.018960465831258648	28ED	Col vs prr7.9	*
	5	prr7,9 I	80.34513274336271	28EN	Col vs prr7.9	0.11769982614260949	28EN	Col vs prr7.9	n.s
	6	prr7,9 II	77.4507177033493	Col	27 EN vs 28 EN	0.5189961799445153	Col	27 EN vs 28 EN	n.s
	7	prr7,9 III	59.5913200723327	prr7.9	27 EN vs 28 EN	0.5930067610455964	prr7.9	27 EN vs 28 EN	n.s
	8	prr7,9 IV	339.6353174603173	Col	28 EN vs 28 ED	0.6160276879003956	Col	28 EN vs 28 ED	n.s
28 EN	9	Col I	75.76208178438651	prr7.9	28 EN vs 28 ED	0.9912742373838004	prr7.9	28 EN vs 28 ED	n.s
	10	Col II	48.58080495356035	Col	27 EN vs 28 ED	0.802073365028804	Col	27 EN vs 28 ED	n.s
	11	Col III	49.92804428044277	prr7.9	27 EN vs 28 ED	0.5990358884307454	prr7.10	27 EN vs 28 ED	n.s
	12	Col IV	47.89128508124068
	13	prr7,9 I	124.83852691218128
	14	prr7,9 II	65.63786982248523
	15	prr7,9 III	105.578021978022
	16	prr7,9 IV	107.23297350343472
28 ED	17	Col I	86.73796791443841
	18	Col II	48.91644908616194
	19	Col III	49.640502512562726
	20	Col IV	60.386673151751026
	21	prr7,9 I	121.26627218934907
	22	prr7,9 II	144.6957792207792
	23	prr7,9 III	61.13113553113548
	24	prr7,9 IV	75.13654891304348

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3400

	42.7257239818573		48.8215506076813
	42.780214548111		48.9199101924896
	42.8366243839264		49.0114510059357
	42.8974747657776		49.0946054458618
	42.9648697376251		49.1681456565857
	43.0399298667908		49.2315649986267
	43.122673034668		49.2851257324219
	43.212378025055		49.3297874927521
	43.3080613613129		49.3672013282776
	43.4087097644806		49.3998348712921
	43.5133457183838		49.4306385517121
	43.6213076114655		49.4625568389893
	43.732625246048		49.4981169700623
	43.8481092453003		49.5391488075257
	43.9688742160797		49.5867490768433
	44.0954923629761		49.641352891922
	44.227546453476		49.7028291225434
	44.3636178970337		49.7709691524506
	44.5016443729401		49.8458921909332
	44.6391105651856		49.9278426170349
	44.7730541229248		50.0166475772858
	44.9003040790558		50.1112520694733
	45.0180590152741		50.2097725868225
	45.124351978302		50.3100752830506
	45.2181935310364		50.4100203514099
	45.2994763851166		50.5072593688965
	45.3687906265259		50.5991458892823
	45.4275608062744		50.6832718849182
	45.4780817031861		50.7584512233734
	45.52321434021		50.825321674347
	45.565927028656		50.8858323097229
	45.6088244915009		50.9422540664673
	45.6539273262024		50.996345281601
	45.7025706768036		51.0492682456971
	45.7552313804627		51.10182762146
	45.8114564418793		51.1543333530426
	45.8700001239777		51.2063145637513
	45.9292054176331		51.2564480304718
	45.9875524044037		51.3030350208283
	46.0438787937165		51.3447403907776
	46.0974395275116		51.3812124729157
	46.1479187011719		51.4128863811493
	46.195387840271		51.4406144618988
	46.2402224540711		51.4653861522675
	46.2829947471619		51.4881134033203
	46.3242113590241		51.5095055103302
	46.3643014431		51.5301644802094
	46.4037775993347		51.5505909919739
	46.4434325695038		51.5714526176453
	46.4844465255738		51.5938043594361
	46.5281069278717		51.6189575195313
	46.5755462646485		51.6481578350067
	46.6276288032532		51.6825675964356
	46.6849982738495		51.7232239246369
	46.7479705810547		51.7707705497742
	46.8163192272186		51.8250524997711
	46.888929605484		51.8846988677979
	46.9641208648682		51.9474983215332
	47.0403671264649		52.0115435123444
	47.1169590950012		52.0759224891663
	47.1941947937012		52.1406948566437
	47.2733914852143		52.2064626216889
	47.3571002483368		52.2741973400116
	47.4499821662903		52.3456037044525
	47.5597858428955		52.4235427379608
	47.6979970932007		52.5118291378022
	47.8801906108856		52.6148617267609
	48.1262028217316		52.7380824089051
	48.4599530696869		52.8892159461975
	48.9084005355835		53.0799090862274
	49.4988262653351		53.3266603946686
	50.2545833587647		53.6506295204163
	51.1897444725037		54.0766537189484
	52.3036718368531		54.6314120292664
	53.5765409469605		55.340588092804
	54.9671292304993		56.2245726585388
	56.4136862754822		57.2929978370667
	57.8384637832642		58.539354801178
	59.155410528183		59.9372804164887
	60.2800190448761		61.4396095275879
	61.1397802829743		62.9806756973267
	61.6834580898285		64.4814372062683
	61.8874073028565		65.8570528030396
	61.757642030716		67.0263051986695
	61.3272368907929		67.9218351840973
	60.6500685214997		68.4990465641022
	59.7922503948212		68.7415361404419
	58.8231801986695		68.661904335022
	57.8076660633087		68.2983040809632
	56.8002581596375		67.7079677581787
	55.842262506485		66.9591009616852
	54.9613475799561		66.1219537258148
	54.1731655597687		65.2604460716248
	53.4841060638428		64.4261121749878
	52.894276380539		63.6551380157471
	52.4000883102417		62.9688143730164
	51.9958317279816		62.3760581016541
	51.6745209693909		61.8767619132996
	51.4282405376435		61.4653110504151
	51.2485325336457		61.1338078975678