Testing thiamine metabolites in Col and prr7prr9

Selected Cell

Cell:

Value:

Read.Me Sheet1 Results-std curve Repeat for thiamin Graph-stats Graph-stats (2) Chromatogram

Testing thiamine vitamers in plant samples from study L&H3
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites

Analysis Protocol	From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight.

Sheets of this workbook	Contents
Sheet1	Raw data and sampling plan
Results-std curve	Data for individual replicates and standard curve
Repeat for thiamin
Graph-stats	Summary statistics, means, t-test statistics except thiamine, and final charts
Graph-stats (2)	Summary statistics, means, t-test statistics only for thiamine
Chromatogram	raw data traces

Credit
Experimental design, plant growth and sampling	Yin Hoon Chew, Andrew Millar group University of Edinburgh
Analytical design, execution and data analysis	Michael Moulin, Teresa Fitzpatrick group University of Geneva
File Curation	Andrew Millar




numbering	Col				prr7,prr9					replicat	Col				prr7,prr9
27 EN	1	2	3	4	5	6	7	8		27 EN	1	2	3	4	1	2	3	4
28 ED	9	10	11	12	13	14	15	16		28 ED	1	2	3	4	1	2	3	4
28 EN	17	18	19	20	21	22	23	24		28 EN	1	2	3	4	1	2	3	4

mass tube	Col				prr7,prr9
27 EN	0.9438	0.9547	0.9455	0.9373	0.9437	0.9561	0.9486	0.9465
28 ED	0.9427	0.9391	0.9409	0.9434	0.9522	0.9437	0.9438	0.9442
28 EN	0.944	0.9421	0.9439	0.9465	0.9562	0.9436	0.9506	0.9442

mass tube+sample	Col				prr7,prr9
27 EN	1.0289	1.0105	0.992	0.9987	1.0115	1.0188	1.0039	1.0221
28 ED	1.0234	1.036	1.0222	1.0111	1.0581	0.9944	0.9984	1.0461
28 EN	1.0562	1.0187	1.0434	1.0493	1.0576	1.0668	1.0598	1.0178

mass sample	Col				prr7,prr9
27 EN	0.08509999999999995	0.05579999999999996	0.046499999999999986	0.06140000000000001	0.06780000000000008	0.06269999999999998	0.055300000000000016	0.0756
28 ED	0.0807000000000001	0.09689999999999999	0.08130000000000004	0.0677000000000001	0.1059	0.05069999999999997	0.05459999999999998	0.10189999999999999
28 EN	0.11220000000000008	0.07659999999999989	0.09950000000000014	0.10279999999999989	0.10140000000000005	0.12319999999999998	0.10920000000000007	0.0736

mass sample	Col				prr7,prr9
27 EN	300	200	200	200	200	200	200	300
28 ED	300	300	300	200	400	200	200	400
28 EN	300	300	300	300	40	400	400	300


	Extraxtion volume	Mass			Sample analysis order		TDP	TMP	THIA	Extraction volume	Mass
1	300	85.09999999999995			0.03125	pmol	98.93505859375	100.81101989746094	217.923583984375
2	200	55.79999999999996			0.0625	pmol	252.35	237.66	289.42
3	200	46.499999999999986			0.125	pmol	309.66	318.61	576.96
4	200	61.40000000000001			0.25	pmol	502.94	515.39	955.81
5	200	67.80000000000008			0.5	pmol	850.95	859.87	1269.7
6	200	62.699999999999974			1	pmol	1621.6	1673.7	2243.1
7	200	55.30000000000002			2	pmol	3490.9	3645	4747
8	300	75.6			4	pmol	6814.1	7077	9161
9	300	80.7000000000001			1	Col I	2180.39501953125	301.9862060546875	83.60588073730469	300	85.09999999999995
10	300	96.89999999999999			9	Col I	1917.8831787109375	375.14276123046875	132.87686157226562	300	80.7000000000001
11	300	81.30000000000004			17	Col I	2178.0859375	806.1314697265625	72.3534927368164	300	112.20000000000007
12	200	67.70000000000009			5	prr7,9 I	2514.615478515625	299.6667175292969	70.3182601928711	200	67.80000000000008
13	400	105.89999999999999			13	prr7,9 I	1365.0078125	1249.138671875	116.79225158691406	400	105.89999999999999
14	200	50.69999999999997			21	prr7,9 I	1654.855712890625	1042.51416015625	116.10993957519531	400	101.40000000000005
15	200	54.59999999999998			Standard	1	1645.3131103515625	1719.94091796875	2256.296630859375
16	400	101.89999999999999			2	Col II	2157.2509765625	176.75811767578125	54.220611572265625	200	55.79999999999996
17	300	112.20000000000007			10	Col II	1865.593994140625	616.8743286132812	59.84711456298828	300	96.89999999999999
18	200	76.5999999999999			18	Col II	2377.778564453125	465.9914245605469	59.2650146484375	200	76.5999999999999
19	300	99.50000000000014			6	prr7,9 II	2416.207275390625	603.93408203125	58.665550231933594	200	62.699999999999974
20	300	102.7999999999999			14	prr7,9 II	2289.3037109375	526.7593383789062	127.31964874267578	200	50.69999999999997
21	400	101.40000000000005			22	prr7,9 II	1362.8040771484375	1686.056884765625	129.24539184570312	400	123.19999999999997
22	400	123.19999999999997			Standard	0.5	808.2450561523438	831.448486328125	1122.8255615234375
23	400	109.20000000000007			3	Col III	2016.7115478515625	173.78077697753906	65.31547546386719	200	46.499999999999986
24	300	73.6			11	Col III	1981.958740234375	174.57398986816406	21.63164520263672	300	81.30000000000004
					19	Col III	2064.210693359375	473.5988464355469	64.27493286132812	300	99.50000000000014
					7	prr7,9 III	2498.947509765625	391.9966735839844	105.10193634033203	200	55.30000000000002
	Derivatization				15	prr7,9 III	2427.84375	588.3184204101562	63.29181671142578	200	54.59999999999998
	25 µL				23	prr7,9 III	1696.642333984375	1158.29	123.79141235351562	400	109.20000000000007
	injection	injection			Standard	1	1606.5908203125	1679.7308349609375	2153.149169921875
	20 µL				4	Col IV	2237.2646484375	125.17689514160156	43.60393142700195	200	61.40000000000001
					12	Col IV	2196.937255859375	269.585	42.28960418701172	200	67.70000000000009
					20	Col IV	2176.550048828125	417.441	73.04740905761719	300	102.7999999999999
					8	prr7,9 IV	2290.056396484375	659.33	345.0472412109375	300	75.6
					16	prr7,9 IV	1518.505859375	1229.6	95.14126586914062	400	101.89999999999999
					24	prr7,9 IV	2028.2176513671875	424.313	69.16194915771484	300	73.6
					Standard	0.25	447.85986328125	458.4064636230469	788.3421020507812
					Standard	0.03125	98.93505859375	100.81101989746094	217.923583984375



Sample analysis order		TDP	TMP	THIA	Extraction volume	Mass
1	Col I	2180.39501953125	301.9862060546875	83.60588073730469	3.0E-4	0.08509999999999995
2	Col II	2157.2509765625	176.75811767578125	54.220611572265625	1.9999999999999998E-4	0.05579999999999996
3	Col III	2016.7115478515625	173.78077697753906	65.31547546386719	1.9999999999999998E-4	0.046499999999999986
4	Col IV	2237.2646484375	125.17689514160156	43.60393142700195	1.9999999999999998E-4	0.06140000000000002
5	prr7,9 I	2514.615478515625	299.6667175292969	70.3182601928711	1.9999999999999998E-4	0.06780000000000008
6	prr7,9 II	2416.207275390625	603.93408203125	58.665550231933594	1.9999999999999998E-4	0.06269999999999998
7	prr7,9 III	2498.947509765625	391.9966735839844	105.10193634033203	1.9999999999999998E-4	0.05530000000000002				pmol	TDP	TMP	Thia
8	prr7,9 IV	2290.056396484375	659.33	345.0472412109375	3.0E-4	0.0756				3.125E-14	98.93505859375	100.81101989746094	217.923583984375
9	Col I	1917.8831787109375	375.14276123046875	132.87686157226562	3.0E-4	0.0807000000000001				6.25E-14	252.35	237.66	289.42
10	Col II	1865.593994140625	616.8743286132812	59.84711456298828	3.0E-4	0.0969				1.25E-13	309.66	318.61	576.96
11	Col III	1981.958740234375	174.57398986816406	21.63164520263672	3.0E-4	0.08130000000000004				2.5E-13	502.94	515.39	955.81
12	Col IV	2196.937255859375	269.585	42.28960418701172	1.9999999999999998E-4	0.0677000000000001				5.0E-13	850.95	859.87	1269.7
13	prr7,9 I	1365.0078125	1249.138671875	116.79225158691406	3.9999999999999996E-4	0.1059				1.0E-12	1621.6	1673.7	2243.1
14	prr7,9 II	2289.3037109375	526.7593383789062	127.31964874267578	1.9999999999999998E-4	0.05069999999999997				2.0E-12	3490.9	3645	4747
15	prr7,9 III	2427.84375	588.3184204101562	63.29181671142578	1.9999999999999998E-4	0.05459999999999998				4.0E-12	6814.1	7077	9161
16	prr7,9 IV	1518.505859375	1229.6	95.14126586914062	3.9999999999999996E-4	0.10189999999999999				Slope	1.685774878178111E15	1.7555770182597208E15	2.2371156417932975E15
17	Col I	2178.0859375	806.1314697265625	72.3534927368164	3.0E-4	0.11220000000000008				b	63.49	54.786	204.24
18	Col II	2377.778564453125	465.9914245605469	59.2650146484375	1.9999999999999998E-4	0.07659999999999989
19	Col III	2064.210693359375	473.5988464355469	64.27493286132812	3.0E-4	0.09950000000000014
20	Col IV	2176.550048828125	417.441	73.04740905761719	3.0E-4	0.1027999999999999
21	prr7,9 I	1654.855712890625	1042.51416015625	116.10993957519531	3.9999999999999996E-4	0.10140000000000005
22	prr7,9 II	1362.8040771484375	1686.056884765625	129.24539184570312	3.9999999999999996E-4	0.12319999999999998
23	prr7,9 III	1696.642333984375	1158.29	123.79141235351562	3.9999999999999996E-4	0.10920000000000007
24	prr7,9 IV	2028.2176513671875	424.313	69.16194915771484	3.0E-4	0.0736


Sample analysis order	pmol injected	TDP	TMP	THIA			Sample analysis order	pmol/µL	TDP	TMP	THIA
1	Col I	1.2557459758915614E-12	1.4080852248779928E-13	4.180294036865234E-14		27 EN	1	Col I	2.511491951783123E-7	2.8161704497559855E-8	8.360588073730468E-9
2	Col II	1.2420169523615854E-12	6.947693915285502E-14	2.7110305786132812E-14			2	Col II	2.4840339047231705E-7	1.3895387830571003E-8	5.422061157226562E-9
3	Col III	1.1586491014520826E-12	6.778100632434624E-14	3.265773773193359E-14			3	Col III	2.3172982029041653E-7	1.3556201264869248E-8	6.531547546386718E-9
4	Col IV	1.289480983835037E-12	4.009558931876374E-14	2.1801965713500978E-14			4	Col IV	2.5789619676700736E-7	8.019117863752749E-9	4.360393142700195E-9
5	prr7,9 I	1.4540052234997483E-12	1.3948731099934524E-13	3.515913009643555E-14			5	prr7,9 I	2.908010446999496E-7	2.7897462199869047E-8	7.0318260192871094E-9
6	prr7,9 II	1.3956295741773702E-12	3.1280204532160763E-13	2.93327751159668E-14			6	prr7,9 II	2.79125914835474E-7	6.256040906432153E-8	5.8665550231933595E-9
7	prr7,9 III	1.4447109998446106E-12	1.9207968096908483E-13	5.2550968170166016E-14			7	prr7,9 III	2.889421999689221E-7	3.8415936193816965E-8	1.0510193634033203E-8
8	prr7,9 IV	1.3207969968627843E-12	3.443562963698831E-13	1.7252362060546875E-13			8	prr7,9 IV	2.6415939937255685E-7	6.887125927397661E-8	3.450472412109375E-8
9	Col I	1.1000242100624132E-12	1.824794685157328E-13	6.643843078613281E-14		28 EN	9	Col I	2.2000484201248262E-7	3.6495893703146554E-8	1.328768615722656E-8
10	Col II	1.0690063171947585E-12	3.2017298174162225E-13	2.992355728149414E-14			10	Col II	2.138012634389517E-7	6.403459634832445E-8	5.9847114562988275E-9
11	Col III	1.1380337701483285E-12	6.823283092809465E-14	1.081582260131836E-14			11	Col III	2.2760675402966568E-7	1.364656618561893E-8	2.1631645202636718E-9
12	Col IV	1.2655588142143173E-12	1.2235236492952458E-13	2.114480209350586E-14			12	Col IV	2.5311176284286343E-7	2.4470472985904917E-8	4.228960418701171E-9
13	prr7,9 I	7.72059086505433E-13	6.803191540174895E-13	5.839612579345703E-14			13	prr7,9 I	1.5441181730108656E-7	1.360638308034979E-7	1.1679225158691405E-8
14	prr7,9 II	1.3203505045365442E-12	2.6884228573849006E-13	6.365982437133789E-14			14	prr7,9 II	2.640701009073088E-7	5.376845714769801E-8	1.2731964874267577E-8
15	prr7,9 III	1.4025323194727273E-12	3.039071569409353E-13	3.164590835571289E-14			15	prr7,9 III	2.8050646389454546E-7	6.078143138818706E-8	6.329181671142578E-9
16	prr7,9 IV	8.63113976966781E-13	6.691896668621105E-13	4.757063293457031E-14			16	prr7,9 IV	1.726227953933562E-7	1.338379333724221E-7	9.514126586914061E-9
17	Col I	1.254376230701299E-12	4.2797636441570696E-13	3.6176746368408206E-14		28 ED	17	Col I	2.5087524614025976E-7	8.559527288314139E-8	7.235349273681641E-9
18	Col II	1.3728337006387685E-12	2.3422807446418336E-13	2.963250732421875E-14			18	Col II	2.745667401277537E-7	4.684561489283667E-8	5.92650146484375E-9
19	Col III	1.1868255478582284E-12	2.3856136306153646E-13	3.213746643066406E-14			19	Col III	2.3736510957164565E-7	4.771227261230729E-8	6.427493286132813E-9
20	Col IV	1.2534651430514847E-12	2.065731074330734E-13	3.652370452880859E-14			20	Col IV	2.5069302861029693E-7	4.1314621486614676E-8	7.304740905761718E-9
21	prr7,9 I	9.439965759903138E-13	5.626230862462367E-13	5.805496978759765E-14			21	prr7,9 I	1.8879931519806277E-7	1.1252461724924733E-7	1.161099395751953E-8
22	prr7,9 II	7.707518328619666E-13	9.291935744195838E-13	6.462269592285156E-14			22	prr7,9 II	1.541503665723933E-7	1.8583871488391673E-7	1.2924539184570312E-8
23	prr7,9 III	9.687843585315452E-13	6.285705432017378E-13	6.189570617675781E-14			23	prr7,9 III	1.9375687170630903E-7	1.2571410864034754E-7	1.2379141235351562E-8
24	prr7,9 IV	1.16547451074283E-12	2.1048748995718058E-13	3.458097457885742E-14			24	prr7,9 IV	2.3309490214856598E-7	4.209749799143611E-8	6.9161949157714844E-9

Sample analysis order	nmol/gFW	TDP	TMP	THIA
1	Col I	885.3673155522177	99.27745416296075	29.473283456159127			Mean		TDP	TMP	THIA
2	Col II	890.3347328756889	49.80425745724377	19.43391095780131			27 EN	Col	903.1105514312983	58.377214678324826	22.800776756334205
3	Col III	996.6873990985658	58.30624199943764	28.09267761886761			28 ED	Col	766.8519602323545	114.14230855984623	22.100128563367562
4	Col IV	840.0527581987208	26.12090509365715	14.203234992508774			28 EN	Col	708.7355014572627	153.90018407206077	18.87911546319028
5	prr7,9 I	857.8201908553074	82.29339881967259	20.742849614416226			27 EN	prr7,9	960.3561214730375	173.5208146716876	53.59775060445553
6	prr7,9 II	890.3537953284659	199.554733857485	18.71309417286558			28 ED	prr7,9	832.5114124761428	368.51248577692866	38.71740225546809
7	prr7,9 III	1044.9989148966433	138.93647809698714	38.01155021350162			28 EN	prr7,9	726.2764745957589	419.8352113851851	40.32534466004294
8	prr7,9 IV	1048.2515848117334	273.2986479126056	136.92350841703868
9	Col I	817.8618662174064	135.6724672979424	49.39660281496856			STDEV		TDP	TMP	THIA
10	Col II	661.9234162196647	198.24952429821812	18.528518440553643			27 EN	Col	33.180008622075476	15.239203092020402	3.626063867843288
11	Col III	839.8773211426773	50.356332788261696	7.982156901341957			28 ED	Col	40.11419122603355	33.36385488473636	9.351753009264181
12	Col IV	747.7452373496694	72.29090985496272	12.4932360966061			28 EN	Col	13.156048540266871	25.54300793249725	1.2250361762140936
13	prr7,9 I	583.2363259719983	513.9332608252989	44.114164905349966			27 EN	prr7,9	50.252659703550506	40.97956675158741	28.11063756404265
14	prr7,9 II	1041.6966505219289	212.10436744654058	50.22471350795891			28 ED	prr7,9	118.28920963749397	87.31786360652637	5.807422031023975
15	prr7,9 III	1027.4962047419249	222.64260581753507	23.18381564521091			28 EN	prr7,9	92.05967843469897	90.15789119686292	4.134412699440083
16	prr7,9 IV	677.6164686687191	525.36970901834	37.346914963352546
17	Col I	670.7894281825121	228.86436599770408	19.345853672945548			Fold vs Col		TDP	TMP	THIA
18	Col II	716.8844389758591	122.31231042516119	15.473894164082918			27 EN	Col
19	Col III	715.6736972009405	143.85609832856449	19.379376742108956			28 ED	Col
20	Col IV	731.5944414697387	120.56796153681334	21.31733727362371			28 EN	Col
21	prr7,9 I	744.7704741540934	443.88409171300697	45.802737505007975			27 EN	prr7,9	1.0633871124094536	2.9724065395691963	2.3506984510764846
22	prr7,9 II	500.4882031571212	603.3724509218076	41.96278956029323			28 ED	prr7,9	1.0856220700327788	3.2285354171167215	1.7519084626342294
23	prr7,9 III	709.7321307923403	460.4912404408331	45.34483968993242			28 EN	prr7,9	1.0247496747410416	2.7279708202857345	2.135976377636316
24	prr7,9 IV	950.1150902794808	171.59306246509283	28.191011884938113



Sample analysis order		THIA	Extraction volume	Mass
1	Col I	285.237	3.0E-4	0.08509999999999995
2	Col II	121.45	1.9999999999999998E-4	0.05579999999999996
3	Col III	160.685	1.9999999999999998E-4	0.046499999999999986
4	Col IV	150.074	1.9999999999999998E-4	0.06140000000000002
5	prr7,9 I	272.37	1.9999999999999998E-4	0.06780000000000008
6	prr7,9 II	242.808	1.9999999999999998E-4	0.06269999999999998
7	prr7,9 III	164.77	1.9999999999999998E-4	0.05530000000000002				pmol	Thia
8	prr7,9 IV	855.881	3.0E-4	0.0756				6.25E-14	291.3258
9	Col I	203.8	3.0E-4	0.0807000000000001				1.25E-13	464.7634
10	Col II	156.916	3.0E-4	0.0969				2.5E-13	711.93347
11	Col III	135.305	3.0E-4	0.08130000000000004				5.0E-13	964.34528
12	Col IV	162.112	1.9999999999999998E-4	0.0677000000000001				1.0E-12	1763.112
13	prr7,9 I	330.51	3.9999999999999996E-4	0.1059				2.0E-12	4222.5888
14	prr7,9 II	166.392	1.9999999999999998E-4	0.05069999999999997				Slope	1.9906732241935485E15
15	prr7,9 III	288.228	1.9999999999999998E-4	0.05459999999999998				b	256.55
16	prr7,9 IV	273.176	3.9999999999999996E-4	0.10189999999999999
17	Col I	324.4	3.0E-4	0.11220000000000008
18	Col II	187.35	1.9999999999999998E-4	0.07659999999999989
19	Col III	164.641	3.0E-4	0.09950000000000014
20	Col IV	206.925	3.0E-4	0.1027999999999999
21	prr7,9 I	307.41	3.9999999999999996E-4	0.10140000000000005
22	prr7,9 II	445.663	3.9999999999999996E-4	0.12319999999999998
23	prr7,9 III	166.888	3.9999999999999996E-4	0.10920000000000007
24	prr7,9 IV	184.335	3.0E-4	0.0736


Sample analysis order	pmol injected	THIA			Sample analysis order	pmol/µL	THIA
1	Col I	1.4261850000000002E-13		27 EN	1	Col I	1.140948E-8
2	Col II	6.0725E-14			2	Col II	4.8579999999999996E-9
3	Col III	8.03425E-14			3	Col III	6.4274E-9
4	Col IV	7.503700000000001E-14			4	Col IV	6.0029600000000005E-9
5	prr7,9 I	1.36185E-13			5	prr7,9 I	1.0894799999999998E-8
6	prr7,9 II	1.21404E-13			6	prr7,9 II	9.71232E-9
7	prr7,9 III	8.238500000000001E-14			7	prr7,9 III	6.5908E-9
8	prr7,9 IV	4.279405E-13			8	prr7,9 IV	3.423524E-8
9	Col I	1.019E-13		28 EN	9	Col I	8.151999999999999E-9
10	Col II	7.8458E-14			10	Col II	6.276639999999999E-9
11	Col III	6.76525E-14			11	Col III	5.4122E-9
12	Col IV	8.1056E-14			12	Col IV	6.4844799999999995E-9
13	prr7,9 I	1.6525499999999999E-13			13	prr7,9 I	1.3220399999999999E-8
14	prr7,9 II	8.3196E-14			14	prr7,9 II	6.65568E-9
15	prr7,9 III	1.4411400000000002E-13			15	prr7,9 III	1.152912E-8
16	prr7,9 IV	1.3658799999999998E-13			16	prr7,9 IV	1.0927039999999999E-8
17	Col I	1.6219999999999998E-13		28 ED	17	Col I	1.2975999999999998E-8
18	Col II	9.3675E-14			18	Col II	7.493999999999999E-9
19	Col III	8.23205E-14			19	Col III	6.58564E-9
20	Col IV	1.034625E-13			20	Col IV	8.277E-9
21	prr7,9 I	1.5370500000000003E-13			21	prr7,9 I	1.2296400000000002E-8
22	prr7,9 II	2.228315E-13			22	prr7,9 II	1.782652E-8
23	prr7,9 III	8.344400000000001E-14			23	prr7,9 III	6.6755200000000005E-9
24	prr7,9 IV	9.216750000000001E-14			24	prr7,9 IV	7.3734E-9

Sample analysis order	nmol/gFW	THIA
1	Col I	40.22143360752058					THIA
2	Col II	17.412186379928322			27 EN	Col	26.207991701355937
3	Col III	27.644731182795706			28 ED	Col	22.216221609963032
4	Col IV	19.55361563517915			28 EN	Col	24.568159266491413
5	prr7,9 I	32.138053097345086			27 EN	prr7,9	55.70224879793622
6	prr7,9 II	30.980287081339725			28 ED	prr7,9	40.32873922161233
7	prr7,9 III	23.83652802893308			28 EN	prr7,9	40.22297358543073
8	prr7,9 IV	135.854126984127
9	Col I	30.3048327137546					THIA
10	Col II	19.432321981424142			27 EN	Col	5.16468526370469
11	Col III	19.971217712177108			28 ED	Col	2.701505439732336
12	Col IV	19.15651403249628			28 EN	Col	3.534897659186522
13	prr7,9 I	49.935410764872515			27 EN	prr7,9	26.780270173884016
14	prr7,9 II	26.2551479289941			28 ED	prr7,9	5.004580445280961
15	prr7,9 III	42.2312087912088			28 EN	prr7,9	7.812557334225081
16	prr7,9 IV	42.893189401373895
17	Col I	34.695187165775366
18	Col II	19.566579634464773
19	Col III	19.856201005025095
20	Col IV	24.15466926070041
21	prr7,9 I	48.50650887573963
22	prr7,9 II	57.8783116883117
23	prr7,9 III	24.452454212454196
24	prr7,9 IV	30.054619565217386

	Sample analysis order	pmol/µL	TDP	TMP	THIA
27 EN	1	Col I	885.3673155522177	99.27745416296075	29.473283456159127
	2	Col II	890.3347328756889	49.80425745724377	19.43391095780131			TDP	TMP	Thia			TDP	TMP	Thia
	3	Col III	996.6873990985658	58.30624199943764	28.09267761886761	27EN	Col vs prr7.9	0.3784950101359991	0.03887304751915922	0.31894335633099896	27EN	Col vs prr7.9	n.s	*	n.s
	4	Col IV	840.0527581987208	26.12090509365715	14.203234992508774	28ED	Col vs prr7.9	0.6179695046170415	0.034584532576066675	0.18190213222393758	28ED	Col vs prr7.9	n.s	*	n.s
	5	prr7,9 I	857.8201908553074	82.29339881967259	20.742849614416226	28EN	Col vs prr7.9	0.856606669838744	0.029646233508172554	0.002517983320163621	28EN	Col vs prr7.9	n.s	*	**
	6	prr7,9 II	890.3537953284659	199.554733857485	18.71309417286558	Col	27 EN vs 28 EN	0.0397230310306063	0.17923948406921422	0.9465796126527969	Col	27 EN vs 28 EN	*	n.s	n.s
	7	prr7,9 III	1044.9989148966433	138.93647809698714	38.01155021350162	prr7.9	27 EN vs 28 EN	0.3582725251207178	0.08970590900428527	0.6227325689414196	prr7.9	27 EN vs 28 EN	n.s	n.s	n.s
	8	prr7,9 IV	1048.2515848117334	273.2986479126056	136.92350841703868	Col	28 EN vs 28 ED	0.21778917569917583	0.3805817643529822	0.7443616614354294	Col	28 EN vs 28 ED	n.s	n.s	n.s
28 EN	9	Col I	817.8618662174064	135.6724672979424	49.39660281496856	prr7.9	28 EN vs 28 ED	0.5050727905942308	0.6967992204231616	0.8290329831982349	prr7.9	28 EN vs 28 ED	n.s	n.s	n.s
	10	Col II	661.9234162196647	198.24952429821812	18.528518440553643	Col	27 EN vs 28 ED	0.0015937499093712326	0.018331131421305904	0.3450625364371603	Col	27 EN vs 28 ED	**	*	n.s
	11	Col III	839.8773211426773	50.356332788261696	7.982156901341957	prr7.9	27 EN vs 28 ED	0.06709678940629217	0.047343595565421566	0.6568931221269751	prr7.10	27 EN vs 28 ED	n.s	*	n.s
	12	Col IV	747.7452373496694	72.29090985496272	12.4932360966061
	13	prr7,9 I	583.2363259719983	513.9332608252989	44.114164905349966
	14	prr7,9 II	1041.6966505219289	212.10436744654058	50.22471350795891
	15	prr7,9 III	1027.4962047419249	222.64260581753507	23.18381564521091
	16	prr7,9 IV	677.6164686687191	525.36970901834	37.346914963352546
28 ED	17	Col I	670.7894281825121	228.86436599770408	19.345853672945548
	18	Col II	716.8844389758591	122.31231042516119	15.473894164082918
	19	Col III	715.6736972009405	143.85609832856449	19.379376742108956
	20	Col IV	731.5944414697387	120.56796153681334	21.31733727362371
	21	prr7,9 I	744.7704741540934	443.88409171300697	45.802737505007975
	22	prr7,9 II	500.4882031571212	603.3724509218076	41.96278956029323
	23	prr7,9 III	709.7321307923403	460.4912404408331	45.34483968993242
	24	prr7,9 IV	950.1150902794808	171.59306246509283	28.191011884938113

	Sample analysis order	pmol/µL	THIA
27 EN	1	Col I	100.55358401880146
	2	Col II	43.530465949820815			Thia			Thia
	3	Col III	69.11182795698926	27EN	Col vs prr7.9	0.3210491931812907	27EN	Col vs prr7.9	n.s
	4	Col IV	48.884039087947876	28ED	Col vs prr7.9	0.018960465831258648	28ED	Col vs prr7.9	*
	5	prr7,9 I	80.34513274336271	28EN	Col vs prr7.9	0.11769982614260949	28EN	Col vs prr7.9	n.s
	6	prr7,9 II	77.4507177033493	Col	27 EN vs 28 EN	0.5189961799445153	Col	27 EN vs 28 EN	n.s
	7	prr7,9 III	59.5913200723327	prr7.9	27 EN vs 28 EN	0.5930067610455964	prr7.9	27 EN vs 28 EN	n.s
	8	prr7,9 IV	339.6353174603173	Col	28 EN vs 28 ED	0.6160276879003956	Col	28 EN vs 28 ED	n.s
28 EN	9	Col I	75.76208178438651	prr7.9	28 EN vs 28 ED	0.9912742373838004	prr7.9	28 EN vs 28 ED	n.s
	10	Col II	48.58080495356035	Col	27 EN vs 28 ED	0.802073365028804	Col	27 EN vs 28 ED	n.s
	11	Col III	49.92804428044277	prr7.9	27 EN vs 28 ED	0.5990358884307454	prr7.10	27 EN vs 28 ED	n.s
	12	Col IV	47.89128508124068
	13	prr7,9 I	124.83852691218128
	14	prr7,9 II	65.63786982248523
	15	prr7,9 III	105.578021978022
	16	prr7,9 IV	107.23297350343472
28 ED	17	Col I	86.73796791443841
	18	Col II	48.91644908616194
	19	Col III	49.640502512562726
	20	Col IV	60.386673151751026
	21	prr7,9 I	121.26627218934907
	22	prr7,9 II	144.6957792207792
	23	prr7,9 III	61.13113553113548
	24	prr7,9 IV	75.13654891304348

2501

2502

2503

2504

2505

2506

2507

2508

2509

2510

2511

2512

2513

2514

2515

2516

2517

2518

2519

2520

2521

2522

2523

2524

2525

2526

2527

2528

2529

2530

2531

2532

2533

2534

2535

2536

2537

2538

2539

2540

2541

2542

2543

2544

2545

2546

2547

2548

2549

2550

2551

2552

2553

2554

2555

2556

2557

2558

2559

2560

2561

2562

2563

2564

2565

2566

2567

2568

2569

2570

2571

2572

2573

2574

2575

2576

2577

2578

2579

2580

2581

2582

2583

2584

2585

2586

2587

2588

2589

2590

2591

2592

2593

2594

2595

2596

2597

2598

2599

2600

17.9857151085767	28.4603118896485	27.4725198745728	29.8607170581818	30.6104898452759	17.9857151085767	178.4603118896485	52.4725198745728	29.8607170581818	105.61048984527591
17.992915154657	28.4753441810608	27.4708926677704	29.8444867134094	30.5884182453156	17.992915154657	178.4753441810608	52.4708926677704	29.8444867134094	105.58841824531561
18.0001152007373	28.4898579120636	27.4695873260498	29.8316478729248	30.5677115917206	18.0001152007373	178.4898579120636	52.469587326049805	29.8316478729248	105.5677115917206
18.0073152468176	28.5034537315369	27.4681389331818	29.82137799263	30.5483639240265	18.0073152468176	178.5034537315369	52.468138933181805	29.82137799263	105.5483639240265
18.0145152928979	28.5161256790161	27.4660170078278	29.8128962516785	30.5303514003754	18.0145152928979	178.5161256790161	52.4660170078278	29.8128962516785	105.5303514003754
18.0217153389782	28.5282194614411	27.4627327919007	29.8053562641144	30.5138647556305	18.0217153389782	178.5282194614411	52.4627327919007	29.8053562641144	105.5138647556305
18.0289153850585	28.5403251647949	27.4582624435425	29.7978281974793	30.4994583129883	18.0289153850585	178.5403251647949	52.458262443542495	29.7978281974793	105.4994583129883
18.0361154311388	28.5532712936402	27.4533152580261	29.7896206378937	30.4880142211914	18.0361154311388	178.5532712936402	52.4533152580261	29.7896206378937	105.4880142211914
18.0433154772191	28.5678803920746	27.4492740631104	29.7804772853852	30.4807364940644	18.0433154772191	178.56788039207458	52.4492740631104	29.7804772853852	105.4807364940644
18.0505155232994	28.5845160484314	27.4477243423462	29.770576953888	30.4789543151856	18.0505155232994	178.5845160484314	52.4477243423462	29.770576953888	105.4789543151856
18.0577155693797	28.6028206348419	27.4500131607056	29.7602415084839	30.4838716983795	18.0577155693797	178.6028206348419	52.450013160705595	29.7602415084839	105.4838716983795
18.06491561546	28.6218404769898	27.4569869041443	29.7496974468231	30.4967343807221	18.06491561546	178.6218404769898	52.4569869041443	29.7496974468231	105.4967343807221
18.0721156615403	28.6403834819794	27.468866109848	29.7390341758728	30.5189371109009	18.0721156615403	178.6403834819794	52.468866109848	29.7390341758728	105.51893711090091
18.0793157076205	28.6572992801666	27.4850904941559	29.7283709049225	30.552089214325	18.0793157076205	178.6572992801666	52.4850904941559	29.7283709049225	105.55208921432501
18.0865157537008	28.6715090274811	27.5041520595551	29.7180831432343	30.597722530365	18.0865157537008	178.6715090274811	52.504152059555096	29.7180831432343	105.597722530365
18.0937157997811	28.6820113658905	27.5237500667572	29.7088921070099	30.6569159030915	18.0937157997811	178.6820113658905	52.5237500667572	29.7088921070099	105.6569159030915
18.1009158458614	28.6880791187287	27.5413155555725	29.701828956604	30.7302951812744	18.1009158458614	178.6880791187287	52.541315555572496	29.701828956604	105.7302951812744
18.1081158919417	28.689593076706	27.5546908378601	29.69810962677	30.8183372020722	18.1081158919417	178.689593076706	52.5546908378601	29.69810962677	105.8183372020722
18.115315938022	28.6870837211609	27.5626182556153	29.6990633010864	30.9215307235718	18.115315938022	178.6870837211609	52.5626182556153	29.6990633010864	105.9215307235718
18.1225159841023	28.6814272403717	27.5648057460785	29.7060668468476	31.0400784015656	18.1225159841023	178.6814272403717	52.5648057460785	29.7060668468476	106.04007840156561
18.1297160301826	28.6734104156494	27.5618672370911	29.7207176685333	31.173574924469	18.1297160301826	178.6734104156494	52.5618672370911	29.7207176685333	106.173574924469
18.1369160762629	28.6635398864746	27.5552690029144	29.7447085380554	31.3209891319275	18.1369160762629	178.6635398864746	52.5552690029144	29.7447085380554	106.3209891319275
18.1441161223432	28.6522686481476	27.5471091270447	29.7795593738556	31.4811706542969	18.1441161223432	178.6522686481476	52.5471091270447	29.7795593738556	106.4811706542969
18.1513161684235	28.6401629447937	27.539736032486	29.8262178897858	31.653106212616	18.1513161684235	178.6401629447937	52.539736032486005	29.8262178897858	106.653106212616
18.1585162145038	28.6278367042542	27.5350987911225	29.8850417137146	31.8357884883881	18.1585162145038	178.6278367042542	52.5350987911225	29.8850417137146	106.8357884883881
18.1657162605841	28.6156952381134	27.5342404842377	29.9561142921448	32.0276916027069	18.1657162605841	178.6156952381134	52.5342404842377	29.9561142921448	107.02769160270691
18.1729163066644	28.60386967659	27.5371491909027	30.0396382808686	32.2264969348908	18.1729163066644	178.60386967659	52.537149190902696	30.0396382808686	107.2264969348908
18.1801163527447	28.5923540592194	27.5429964065552	30.1360487937927	32.4289858341217	18.1801163527447	178.5923540592194	52.542996406555204	30.1360487937927	107.4289858341217
18.187316398825	28.5812854766846	27.5504112243653	30.2457392215729	32.6310276985169	18.187316398825	178.5812854766846	52.5504112243653	30.2457392215729	107.6310276985169
18.1945164449053	28.5710215568543	27.5577068328858	30.3687810897827	32.8274250030518	18.1945164449053	178.5710215568543	52.5577068328858	30.3687810897827	107.8274250030518
18.2017164909856	28.5618305206299	27.5632202625275	30.5049240589142	33.0118179321289	18.2017164909856	178.5618305206299	52.5632202625275	30.5049240589142	108.0118179321289
18.2089165370659	28.5537123680115	27.5656878948212	30.6536197662354	33.1770122051239	18.2089165370659	178.5537123680115	52.565687894821195	30.6536197662354	108.1770122051239
18.2161165831462	28.5463333129883	27.5646686553955	30.8139383792877	33.3154857158661	18.2161165831462	178.5463333129883	52.5646686553955	30.8139383792877	108.3154857158661
18.2233166292264	28.5392463207245	27.5606215000153	30.9841811656952	33.4200918674469	18.2233166292264	178.5392463207245	52.5606215000153	30.9841811656952	108.4200918674469
18.2305166753067	28.5319745540619	27.5545597076416	31.1614155769348	33.4845840930939	18.2305166753067	178.5319745540619	52.5545597076416	31.1614155769348	108.4845840930939
18.237716721387	28.5241186618805	27.5476455688477	31.3413143157959	33.5042715072632	18.237716721387	178.5241186618805	52.5476455688477	31.3413143157959	108.5042715072632
18.2449167674673	28.5152971744538	27.5408864021301	31.5183401107788	33.4765911102295	18.2449167674673	178.5152971744538	52.5408864021301	31.5183401107788	108.47659111022949
18.2521168135476	28.5053312778473	27.5349497795105	31.6860496997833	33.40163230896	18.2521168135476	178.5053312778473	52.5349497795105	31.6860496997833	108.40163230895999
18.2593168596279	28.4943521022797	27.5300741195679	31.83753490448	33.2821190357208	18.2593168596279	178.4943521022797	52.5300741195679	31.83753490448	108.2821190357208
18.2665169057082	28.4828364849091	27.5260031223297	31.9658577442169	33.1231236457825	18.2665169057082	178.48283648490911	52.5260031223297	31.9658577442169	108.1231236457825
18.2737169517885	28.4714818000794	27.5220036506653	32.0645391941071	32.9316735267639	18.2737169517885	178.4714818000794	52.5220036506653	32.0645391941071	107.9316735267639
18.2809169978688	28.4610569477082	27.5172591209412	32.1281850337982	32.7163994312287	18.2809169978688	178.4610569477082	52.517259120941205	32.1281850337982	107.7163994312287
18.2881170439491	28.4523725509644	27.5112211704254	32.1530520915985	32.4871420860291	18.2881170439491	178.4523725509644	52.5112211704254	32.1530520915985	107.48714208602911
18.2953170900294	28.4460186958313	27.5038480758667	32.1374535560608	32.2542786598206	18.2953170900294	178.4460186958313	52.5038480758667	32.1374535560608	107.2542786598206
18.3025171361097	28.4421741962433	27.4956524372101	32.0821583271027	32.0278286933899	18.3025171361097	178.4421741962433	52.4956524372101	32.0821583271027	107.02782869338989
18.30971718219	28.4404575824738	27.4874985218048	31.9903790950775	31.8166196346283	18.30971718219	178.4404575824738	52.487498521804795	31.9903790950775	106.8166196346283
18.3169172282703	28.4399688243866	27.4803817272186	31.8673968315125	31.6279828548432	18.3169172282703	178.4399688243866	52.4803817272186	31.8673968315125	106.6279828548432
18.3241172743506	28.4397721290589	27.475243806839	31.7200481891632	31.4676344394684	18.3241172743506	178.4397721290589	52.475243806839	31.7200481891632	106.4676344394684
18.3313173204309	28.4392416477204	27.4726688861847	31.5561532974243	31.3396334648133	18.3313173204309	178.4392416477204	52.4726688861847	31.5561532974243	106.3396334648133
18.3385173665112	28.4383356571198	27.4726986885071	31.3841998577118	31.2463343143463	18.3385173665112	178.4383356571198	52.4726986885071	31.3841998577118	106.2463343143463
18.3457174125915	28.4375786781311	27.4748265743256	31.2129735946655	31.188303232193	18.3457174125915	178.4375786781311	52.474826574325604	31.2129735946655	106.188303232193
18.3529174586718	28.4378290176392	27.4781584739685	31.0510277748108	31.1648488044739	18.3529174586718	178.4378290176392	52.4781584739685	31.0510277748108	106.1648488044739
18.3601175047521	28.4399569034577	27.4817049503327	30.905944108963	31.1746120452881	18.3601175047521	178.4399569034577	52.4817049503327	30.905944108963	106.1746120452881
18.3673175508323	28.4445345401764	27.4846076965332	30.7839572429657	31.2161207199097	18.3673175508323	178.4445345401764	52.4846076965332	30.7839572429657	106.2161207199097
18.3745175969126	28.4517645835877	27.4863183498383	30.6900382041931	31.2879502773285	18.3745175969126	178.4517645835877	52.4863183498383	30.6900382041931	106.28795027732849
18.3817176429929	28.4613609313965	27.4866819381714	30.6281685829163	31.3887357711792	18.3817176429929	178.46136093139648	52.4866819381714	30.6281685829163	106.3887357711792
18.3889176890732	28.4725785255432	27.4859786033631	30.6014001369477	31.5173804759979	18.3889176890732	178.4725785255432	52.4859786033631	30.6014001369477	106.5173804759979
18.3961177351535	28.4841477870941	27.4846732616425	30.6116759777069	31.6732227802277	18.3961177351535	178.4841477870941	52.4846732616425	30.6116759777069	106.6732227802277
18.4033177812338	28.4943640232086	27.4832010269165	30.6597590446472	31.8559110164643	18.4033177812338	178.4943640232086	52.483201026916504	30.6597590446472	106.8559110164643
18.4105178273141	28.5017192363739	27.4819135665894	30.7455241680145	32.0650100708008	18.4105178273141	178.5017192363739	52.4819135665894	30.7455241680145	107.06501007080081
18.4177178733944	28.5055577754975	27.4812579154968	30.8685004711151	32.2995245456696	18.4177178733944	178.5055577754975	52.4812579154968	30.8685004711151	107.2995245456696
18.4249179194747	28.5063207149506	27.4818480014801	31.0282707214356	32.5578570365906	18.4249179194747	178.5063207149506	52.4818480014801	31.0282707214356	107.5578570365906
18.432117965555	28.5052418708801	27.484267950058	31.224536895752	32.8380584716797	18.432117965555	178.5052418708801	52.484267950058	31.224536895752	107.8380584716797
18.4393180116353	28.5037577152252	27.4886250495911	31.4569413661957	33.1377983093262	18.4393180116353	178.5037577152252	52.4886250495911	31.4569413661957	108.1377983093262
18.4465180577156	28.5031378269196	27.494478225708	31.725162267685	33.4540724754334	18.4465180577156	178.5031378269196	52.494478225708	31.725162267685	108.4540724754334
18.4537181037959	28.504490852356	27.5011539459229	32.0290386676789	33.7829291820526	18.4537181037959	178.504490852356	52.5011539459229	32.0290386676789	108.7829291820526
18.4609181498762	28.5087525844574	27.5082588195801	32.3686480522156	34.1196298599243	18.4609181498762	178.5087525844574	52.5082588195801	32.3686480522156	109.1196298599243
18.4681181959565	28.5164058208466	27.5157153606415	32.7443420886994	34.4591081142426	18.4681181959565	178.51640582084661	52.5157153606415	32.7443420886994	109.45910811424261
18.4753182420368	28.527170419693	27.5234997272492	33.1563949584961	34.7961246967316	18.4753182420368	178.527170419693	52.5234997272492	33.1563949584961	109.7961246967316
18.4825182881171	28.5400152206421	27.5312960147858	33.6045801639557	35.1251423358917	18.4825182881171	178.5400152206421	52.531296014785795	33.6045801639557	110.1251423358917
18.4897183341974	28.5534739494324	27.5387227535248	34.0877830982208	35.4400932788849	18.4897183341974	178.5534739494324	52.5387227535248	34.0877830982208	110.4400932788849
18.4969183802777	28.5661399364472	27.5458097457886	34.6038520336151	35.7345700263977	18.4969183802777	178.5661399364472	52.5458097457886	34.6038520336151	110.7345700263977
18.5041184263579	28.5769283771515	27.5531232357025	35.1493120193482	36.0022962093353	18.5041184263579	178.5769283771515	52.5531232357025	35.1493120193482	111.0022962093353
18.5113184724382	28.5851418972016	27.5613486766815	35.7192814350128	36.2374305725098	18.5113184724382	178.5851418972016	52.561348676681504	35.7192814350128	111.2374305725098
18.5185185185185	28.5904109477997	27.570790052414	36.3073348999024	36.4346385002136	18.5185185185185	178.5904109477997	52.570790052414	36.3073348999024	111.4346385002136
18.5257185645988	28.592711687088	27.5812447071076	36.9053304195404	36.5890860557556	18.5257185645988	178.592711687088	52.5812447071076	36.9053304195404	111.5890860557556
18.5329186106791	28.5924077033997	27.5923609733582	37.5037729740143	36.696720123291	18.5329186106791	178.59240770339972	52.5923609733582	37.5037729740143	111.696720123291
18.5401186567594	28.5902321338654	27.6040017604828	38.0921840667725	36.7548763751984	18.5401186567594	178.5902321338654	52.6040017604828	38.0921840667725	111.75487637519839
18.5473187028397	28.5872399806977	27.6162326335907	38.6595904827118	36.7625534534455	18.5473187028397	178.5872399806977	52.6162326335907	38.6595904827118	111.76255345344549
18.55451874892	28.5846054553986	27.6290714740753	39.1950249671936	36.7205023765564	18.55451874892	178.5846054553986	52.6290714740753	39.1950249671936	111.7205023765564
18.5617187950003	28.5833775997162	27.6424050331116	39.6879971027374	36.6310298442841	18.5617187950003	178.5833775997162	52.6424050331116	39.6879971027374	111.6310298442841
18.5689188410806	28.5843193531037	27.6560544967652	40.1287794113159	36.49782538414	18.5689188410806	178.5843193531037	52.6560544967652	40.1287794113159	111.49782538414
18.5761188871609	28.5879194736481	27.6698589324951	40.5086934566498	36.3258957862854	18.5761188871609	178.5879194736481	52.6698589324951	40.5086934566498	111.3258957862854
18.5833189332412	28.5945594310761	27.6835143566132	40.8201992511749	36.1211836338043	18.5833189332412	178.5945594310761	52.6835143566132	40.8201992511749	111.12118363380429
18.5905189793215	28.6045014858246	27.6964068412781	41.0569846630097	35.8900010585785	18.5905189793215	178.60450148582458	52.696406841278105	41.0569846630097	110.89000105857849
18.5977190254018	28.6178410053253	27.707701921463	41.2141084671021	35.6384515762329	18.5977190254018	178.6178410053253	52.707701921463	41.2141084671021	110.63845157623291
18.6049190714821	28.6343514919281	27.7165472507477	41.2881910800934	35.3722035884857	18.6049190714821	178.6343514919281	52.716547250747695	41.2881910800934	110.37220358848569
18.6121191175624	28.6533951759339	27.7222275733948	41.2774026393891	35.0964069366455	18.6121191175624	178.6533951759339	52.722227573394804	41.2774026393891	110.09640693664551
18.6193191636427	28.6740422248841	27.7242422103882	41.1815762519837	34.8157584667206	18.6193191636427	178.6740422248841	52.7242422103882	41.1815762519837	109.81575846672061
18.626519209723	28.6950886249542	27.722430229187	41.0022616386414	34.5342874526978	18.626519209723	178.6950886249542	52.722430229187	41.0022616386414	109.5342874526978
18.6337192558033	28.7150859832764	27.7170360088349	40.7428026199341	34.2552363872528	18.6337192558033	178.7150859832764	52.717036008834896	40.7428026199341	109.25523638725281
18.6409193018836	28.7324547767639	27.7088046073914	40.4085278511048	33.9811503887177	18.6409193018836	178.7324547767639	52.7088046073914	40.4085278511048	108.98115038871771
18.6481193479638	28.7458240985871	27.6986658573151	40.0067567825318	33.7141394615174	18.6481193479638	178.7458240985871	52.6986658573151	40.0067567825318	108.71413946151739
18.6553193940441	28.7543118000031	27.687418460846	39.5465314388275	33.4559738636017	18.6553193940441	178.7543118000031	52.687418460846004	39.5465314388275	108.4559738636017
18.6625194401244	28.7576079368592	27.6756346225739	39.0383005142212	33.2078933715821	18.6625194401244	178.7576079368592	52.675634622573895	39.0383005142212	108.2078933715821
18.6697194862047	28.7560641765595	27.6637852191925	38.4933650493622	32.9704344272614	18.6697194862047	178.7560641765595	52.663785219192505	38.4933650493622	107.97043442726141
18.676919532285	28.7505745887757	27.6524007320404	37.9232704639435	32.7434718608856	18.676919532285	178.7505745887757	52.652400732040405	37.9232704639435	107.74347186088559
18.6841195783653	28.7425100803375	27.6421010494232	37.3393058776856	32.5265765190125	18.6841195783653	178.7425100803375	52.642101049423204	37.3393058776856	107.52657651901251
18.6913196244456	28.7334263324738	27.6334285736084	36.7520689964295	32.3193192481995	18.6913196244456	178.7334263324738	52.6334285736084	36.7520689964295	107.31931924819949
18.6985196705259	28.7246227264405	27.6266872882843	36.1710667610169	32.1213006973267	18.6985196705259	178.7246227264405	52.6266872882843	36.1710667610169	107.1213006973267