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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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17.2657105005472 | 32.1334481239319 | 28.9189338684082 | 29.5792043209076 | 30.091655254364 | 17.2657105005472 | 182.13344812393188 | 53.9189338684082 | 29.5792043209076 | 105.091655254364 | |
17.2729105466275 | 31.7894995212555 | 28.7406027317047 | 29.6092808246613 | 30.075603723526 | 17.2729105466275 | 181.7894995212555 | 53.7406027317047 | 29.6092808246613 | 105.075603723526 | |
17.2801105927078 | 31.4870178699494 | 28.5829722881317 | 29.6389520168305 | 30.057293176651 | 17.2801105927078 | 181.4870178699494 | 53.5829722881317 | 29.6389520168305 | 105.057293176651 | |
17.2873106387881 | 31.2198042869568 | 28.4435033798218 | 29.6662449836731 | 30.0376534461975 | 17.2873106387881 | 181.2198042869568 | 53.4435033798218 | 29.6662449836731 | 105.0376534461975 | |
17.2945106848684 | 30.9821128845215 | 28.3198773860932 | 29.6894133090973 | 30.0182163715363 | 17.2945106848684 | 180.98211288452148 | 53.3198773860932 | 29.6894133090973 | 105.0182163715363 | |
17.3017107309487 | 30.7687342166901 | 28.2103359699249 | 29.7071576118469 | 30.0008237361908 | 17.3017107309487 | 180.7687342166901 | 53.2103359699249 | 29.7071576118469 | 105.0008237361908 | |
17.308910777029 | 30.575156211853 | 28.1135976314545 | 29.7187089920044 | 29.9873590469361 | 17.308910777029 | 180.575156211853 | 53.113597631454496 | 29.7187089920044 | 104.98735904693609 | |
17.3161108231093 | 30.3977072238922 | 28.0286431312561 | 29.7239005565644 | 29.979532957077 | 17.3161108231093 | 180.3977072238922 | 53.0286431312561 | 29.7239005565644 | 104.979532957077 | |
17.3233108691896 | 30.2338004112244 | 27.9545187950134 | 29.7232508659363 | 29.9787938594818 | 17.3233108691896 | 180.2338004112244 | 52.9545187950134 | 29.7232508659363 | 104.9787938594818 | |
17.3305109152699 | 30.0820052623749 | 27.890408039093 | 29.7180056571961 | 29.9861907958985 | 17.3305109152699 | 180.0820052623749 | 52.890408039093 | 29.7180056571961 | 104.9861907958985 | |
17.3377109613502 | 29.941987991333 | 27.8356909751892 | 29.7098219394684 | 30.0017774105072 | 17.3377109613502 | 179.941987991333 | 52.835690975189195 | 29.7098219394684 | 105.0017774105072 | |
17.3449110074305 | 29.8141300678253 | 27.7898728847504 | 29.7004401683808 | 30.0242960453034 | 17.3449110074305 | 179.8141300678253 | 52.7898728847504 | 29.7004401683808 | 105.0242960453034 | |
17.3521110535108 | 29.6990990638733 | 27.7524292469025 | 29.6911478042603 | 30.0512671470642 | 17.3521110535108 | 179.6990990638733 | 52.7524292469025 | 29.6911478042603 | 105.0512671470642 | |
17.3593110995911 | 29.5974791049957 | 27.722716331482 | 29.6827435493469 | 30.0796508789063 | 17.3593110995911 | 179.5974791049957 | 52.722716331482005 | 29.6827435493469 | 105.0796508789063 | |
17.3665111456714 | 29.509449005127 | 27.7001023292542 | 29.6756982803345 | 30.1065981388092 | 17.3665111456714 | 179.509449005127 | 52.7001023292542 | 29.6756982803345 | 105.1065981388092 | |
17.3737111917517 | 29.4346690177918 | 27.6841342449188 | 29.6703815460205 | 30.1298201084137 | 17.3737111917517 | 179.4346690177918 | 52.684134244918795 | 29.6703815460205 | 105.1298201084137 | |
17.3809112378319 | 29.3722867965698 | 27.6745915412903 | 29.6671211719513 | 30.1475167274475 | 17.3809112378319 | 179.3722867965698 | 52.6745915412903 | 29.6671211719513 | 105.1475167274475 | |
17.3881112839122 | 29.3208539485932 | 27.671366930008 | 29.666018486023 | 30.1583409309387 | 17.3881112839122 | 179.3208539485932 | 52.671366930008 | 29.666018486023 | 105.15834093093869 | |
17.3953113299925 | 29.2785227298737 | 27.6743769645691 | 29.6668887138367 | 30.1616609096527 | 17.3953113299925 | 179.2785227298737 | 52.6743769645691 | 29.6668887138367 | 105.1616609096527 | |
17.4025113760728 | 29.2430579662323 | 27.6834428310394 | 29.669326543808 | 30.1577508449555 | 17.4025113760728 | 179.2430579662323 | 52.6834428310394 | 29.669326543808 | 105.1577508449555 | |
17.4097114221531 | 29.2120575904846 | 27.6982307434082 | 29.6729505062103 | 30.1477253437042 | 17.4097114221531 | 179.2120575904846 | 52.6982307434082 | 29.6729505062103 | 105.1477253437042 | |
17.4169114682334 | 29.1832447052002 | 27.7180671691895 | 29.6774029731751 | 30.1330029964447 | 17.4169114682334 | 179.1832447052002 | 52.718067169189496 | 29.6774029731751 | 105.1330029964447 | |
17.4241115143137 | 29.1547656059265 | 27.7418494224549 | 29.6822130680084 | 30.114895105362 | 17.4241115143137 | 179.1547656059265 | 52.741849422454905 | 29.6822130680084 | 105.114895105362 | |
17.431311560394 | 29.1252732276917 | 27.7679800987244 | 29.6866476535797 | 30.0945222377777 | 17.431311560394 | 179.1252732276917 | 52.7679800987244 | 29.6866476535797 | 105.0945222377777 | |
17.4385116064743 | 29.0939271450043 | 27.7944803237915 | 29.6897470951081 | 30.0730884075165 | 17.4385116064743 | 179.0939271450043 | 52.794480323791504 | 29.6897470951081 | 105.07308840751651 | |
17.4457116525546 | 29.060310125351 | 27.8192400932312 | 29.6905517578125 | 30.0519108772278 | 17.4457116525546 | 179.060310125351 | 52.8192400932312 | 29.6905517578125 | 105.0519108772278 | |
17.4529116986349 | 29.0244102478028 | 27.8402209281922 | 29.6883940696717 | 30.0322473049164 | 17.4529116986349 | 179.0244102478028 | 52.840220928192196 | 29.6883940696717 | 105.0322473049164 | |
17.4601117447152 | 28.9867341518402 | 27.8558254241944 | 29.6828866004944 | 30.015116930008 | 17.4601117447152 | 178.9867341518402 | 52.8558254241944 | 29.6828866004944 | 105.01511693000799 | |
17.4673117907955 | 28.9482891559601 | 27.8651177883148 | 29.6738803386688 | 30.0012171268463 | 17.4673117907955 | 178.9482891559601 | 52.865117788314805 | 29.6738803386688 | 105.0012171268463 | |
17.4745118368758 | 28.9102971553803 | 27.867740392685 | 29.661238193512 | 29.9909710884094 | 17.4745118368758 | 178.9102971553803 | 52.867740392685 | 29.661238193512 | 104.9909710884094 | |
17.4817118829561 | 28.8739502429962 | 27.863746881485 | 29.6449661254883 | 29.9845337867737 | 17.4817118829561 | 178.87395024299622 | 52.863746881485 | 29.6449661254883 | 104.9845337867737 | |
17.4889119290364 | 28.8401961326599 | 27.8533220291138 | 29.6253979206085 | 29.9816250801087 | 17.4889119290364 | 178.8401961326599 | 52.8533220291138 | 29.6253979206085 | 104.9816250801087 | |
17.4961119751167 | 28.8097023963928 | 27.836811542511 | 29.6034336090088 | 29.9814939498902 | 17.4961119751167 | 178.8097023963928 | 52.836811542511 | 29.6034336090088 | 104.9814939498902 | |
17.503312021197 | 28.7828147411347 | 27.8149008750916 | 29.5804738998413 | 29.9830198287964 | 17.503312021197 | 178.7828147411347 | 52.814900875091595 | 29.5804738998413 | 104.9830198287964 | |
17.5105120672772 | 28.7594735622406 | 27.788507938385 | 29.55801486969 | 29.9849212169647 | 17.5105120672772 | 178.7594735622406 | 52.788507938384996 | 29.55801486969 | 104.9849212169647 | |
17.5177121133575 | 28.7391185760498 | 27.7584195137024 | 29.5373022556305 | 29.9858868122101 | 17.5177121133575 | 178.7391185760498 | 52.7584195137024 | 29.5373022556305 | 104.9858868122101 | |
17.5249121594378 | 28.7208437919617 | 27.7249693870545 | 29.5191049575806 | 29.9847185611725 | 17.5249121594378 | 178.7208437919617 | 52.7249693870545 | 29.5191049575806 | 104.9847185611725 | |
17.5321122055181 | 28.7035584449768 | 27.6882112026215 | 29.5038282871247 | 29.9805223941803 | 17.5321122055181 | 178.7035584449768 | 52.6882112026215 | 29.5038282871247 | 104.9805223941803 | |
17.5393122515984 | 28.6861002445221 | 27.6485085487366 | 29.491651058197 | 29.9729764461518 | 17.5393122515984 | 178.6861002445221 | 52.6485085487366 | 29.491651058197 | 104.9729764461518 | |
17.5465122976787 | 28.6673605442047 | 27.6068985462189 | 29.4825375080109 | 29.9624681472779 | 17.5465122976787 | 178.6673605442047 | 52.6068985462189 | 29.4825375080109 | 104.9624681472779 | |
17.553712343759 | 28.6463916301728 | 27.5649845600128 | 29.4761002063751 | 29.9501955509186 | 17.553712343759 | 178.6463916301728 | 52.5649845600128 | 29.4761002063751 | 104.95019555091861 | |
17.5609123898393 | 28.6227107048035 | 27.5243222713471 | 29.4717252254486 | 29.9380600452423 | 17.5609123898393 | 178.6227107048035 | 52.5243222713471 | 29.4717252254486 | 104.9380600452423 | |
17.5681124359196 | 28.5964906215668 | 27.4861514568329 | 29.4689357280731 | 29.9286127090454 | 17.5681124359196 | 178.5964906215668 | 52.4861514568329 | 29.4689357280731 | 104.9286127090454 | |
17.5753124819999 | 28.5684049129486 | 27.4514079093933 | 29.4677019119263 | 29.9248278141022 | 17.5753124819999 | 178.5684049129486 | 52.451407909393296 | 29.4677019119263 | 104.9248278141022 | |
17.5825125280802 | 28.5393178462982 | 27.4209201335907 | 29.4685065746308 | 29.9297690391541 | 17.5825125280802 | 178.5393178462982 | 52.4209201335907 | 29.4685065746308 | 104.9297690391541 | |
17.5897125741605 | 28.5100758075714 | 27.3954212665558 | 29.4720232486725 | 29.9461841583252 | 17.5897125741605 | 178.5100758075714 | 52.3954212665558 | 29.4720232486725 | 104.9461841583252 | |
17.5969126202408 | 28.481525182724 | 27.3752272129059 | 29.4786393642426 | 29.9761176109314 | 17.5969126202408 | 178.481525182724 | 52.3752272129059 | 29.4786393642426 | 104.9761176109314 | |
17.6041126663211 | 28.4546077251435 | 27.3599743843079 | 29.488217830658 | 30.0206542015076 | 17.6041126663211 | 178.4546077251435 | 52.359974384307904 | 29.488217830658 | 105.0206542015076 | |
17.6113127124014 | 28.4302651882172 | 27.3487687110901 | 29.5003890991211 | 30.0796210765839 | 17.6113127124014 | 178.4302651882172 | 52.3487687110901 | 29.5003890991211 | 105.0796210765839 | |
17.6185127584817 | 28.4091472625733 | 27.340704202652 | 29.5147895812988 | 30.1516652107239 | 17.6185127584817 | 178.4091472625733 | 52.340704202652 | 29.5147895812988 | 105.1516652107239 | |
17.625712804562 | 28.391569852829 | 27.3352265357971 | 29.5313656330109 | 30.2346825599671 | 17.625712804562 | 178.391569852829 | 52.335226535797105 | 29.5313656330109 | 105.2346825599671 | |
17.6329128506423 | 28.3776700496674 | 27.332079410553 | 29.5503973960877 | 30.3264677524567 | 17.6329128506423 | 178.37767004966742 | 52.332079410553 | 29.5503973960877 | 105.3264677524567 | |
17.6401128967226 | 28.3677220344544 | 27.3310840129853 | 29.5723199844361 | 30.4253220558167 | 17.6401128967226 | 178.3677220344544 | 52.3310840129853 | 29.5723199844361 | 105.42532205581671 | |
17.6473129428029 | 28.3622860908509 | 27.3318529129029 | 29.5976340770722 | 30.5302083492279 | 17.6473129428029 | 178.3622860908509 | 52.3318529129029 | 29.5976340770722 | 105.5302083492279 | |
17.6545129888831 | 28.3619046211243 | 27.334076166153 | 29.6270906925202 | 30.6407272815705 | 17.6545129888831 | 178.3619046211243 | 52.334076166153 | 29.6270906925202 | 105.64072728157049 | |
17.6617130349634 | 28.3667445182801 | 27.3378193378449 | 29.661762714386 | 30.757200717926 | 17.6617130349634 | 178.3667445182801 | 52.3378193378449 | 29.661762714386 | 105.757200717926 | |
17.6689130810437 | 28.3766031265259 | 27.343612909317 | 29.7029435634613 | 30.8807969093323 | 17.6689130810437 | 178.3766031265259 | 52.343612909317 | 29.7029435634613 | 105.8807969093323 | |
17.676113127124 | 28.3909976482392 | 27.3521065711975 | 29.7519028186798 | 31.0130417346955 | 17.676113127124 | 178.3909976482392 | 52.352106571197496 | 29.7519028186798 | 106.01304173469549 | |
17.6833131732043 | 28.4092962741852 | 27.3636221885681 | 29.8095285892487 | 31.1548113822937 | 17.6833131732043 | 178.4092962741852 | 52.3636221885681 | 29.8095285892487 | 106.1548113822937 | |
17.6905132192846 | 28.430724143982 | 27.3781955242157 | 29.8764824867249 | 31.3053250312805 | 17.6905132192846 | 178.430724143982 | 52.3781955242157 | 29.8764824867249 | 106.3053250312805 | |
17.6977132653649 | 28.45419049263 | 27.3958683013916 | 29.9536526203156 | 31.46191239357 | 17.6977132653649 | 178.45419049263 | 52.3958683013916 | 29.9536526203156 | 106.46191239357 | |
17.7049133114452 | 28.4784257411957 | 27.4168312549591 | 30.0422847270966 | 31.6206216812134 | 17.7049133114452 | 178.4784257411957 | 52.4168312549591 | 30.0422847270966 | 106.62062168121341 | |
17.7121133575255 | 28.5022914409638 | 27.4412989616394 | 30.1436483860016 | 31.7767679691315 | 17.7121133575255 | 178.5022914409638 | 52.441298961639404 | 30.1436483860016 | 106.7767679691315 | |
17.7193134036058 | 28.5249829292298 | 27.4691104888916 | 30.2584052085877 | 31.9251298904419 | 17.7193134036058 | 178.5249829292298 | 52.4691104888916 | 30.2584052085877 | 106.9251298904419 | |
17.7265134496861 | 28.5459101200104 | 27.4996340274811 | 30.3861320018769 | 32.0599436759949 | 17.7265134496861 | 178.5459101200104 | 52.4996340274811 | 30.3861320018769 | 107.0599436759949 | |
17.7337134957664 | 28.5646140575409 | 27.5319755077362 | 30.5252075195313 | 32.1755528450012 | 17.7337134957664 | 178.5646140575409 | 52.5319755077362 | 30.5252075195313 | 107.17555284500119 | |
17.7409135418467 | 28.5806238651276 | 27.565348148346 | 30.6729674339295 | 32.2673320770264 | 17.7409135418467 | 178.5806238651276 | 52.565348148346004 | 30.6729674339295 | 107.2673320770264 | |
17.748113587927 | 28.5935401916504 | 27.599036693573 | 30.8257341384888 | 32.3323249816895 | 17.748113587927 | 178.5935401916504 | 52.599036693573 | 30.8257341384888 | 107.33232498168951 | |
17.7553136340073 | 28.6032021045685 | 27.632337808609 | 30.9789955615998 | 32.3691546916962 | 17.7553136340073 | 178.6032021045685 | 52.632337808609 | 30.9789955615998 | 107.3691546916962 | |
17.7625136800876 | 28.6095976829529 | 27.6645243167877 | 31.1277747154236 | 32.3775947093964 | 17.7625136800876 | 178.6095976829529 | 52.6645243167877 | 31.1277747154236 | 107.37759470939639 | |
17.7697137261679 | 28.6126494407654 | 27.6950538158417 | 31.2671542167664 | 32.3582768440247 | 17.7697137261679 | 178.6126494407654 | 52.6950538158417 | 31.2671542167664 | 107.3582768440247 | |
17.7769137722482 | 28.6122143268585 | 27.7238070964813 | 31.3927173614502 | 32.3128402233124 | 17.7769137722482 | 178.6122143268585 | 52.723807096481295 | 31.3927173614502 | 107.3128402233124 | |
17.7841138183285 | 28.6082267761231 | 27.7509272098541 | 31.5008223056793 | 32.2439074516297 | 17.7841138183285 | 178.6082267761231 | 52.7509272098541 | 31.5008223056793 | 107.2439074516297 | |
17.7913138644088 | 28.6008715629578 | 27.7765572071076 | 31.5886080265045 | 32.154792547226 | 17.7913138644088 | 178.6008715629578 | 52.7765572071076 | 31.5886080265045 | 107.15479254722601 | |
17.798513910489 | 28.590589761734 | 27.8006255626679 | 31.6539525985718 | 32.0492148399353 | 17.798513910489 | 178.590589761734 | 52.8006255626679 | 31.6539525985718 | 107.0492148399353 | |
17.8057139565693 | 28.5779476165772 | 27.8228521347046 | 31.6954135894776 | 31.9312334060669 | 17.8057139565693 | 178.5779476165772 | 52.822852134704604 | 31.6954135894776 | 106.9312334060669 | |
17.8129140026496 | 28.5634756088257 | 27.8427183628082 | 31.7120552062988 | 31.8052411079407 | 17.8129140026496 | 178.5634756088257 | 52.8427183628082 | 31.7120552062988 | 106.8052411079407 | |
17.8201140487299 | 28.547751903534 | 27.8594374656677 | 31.703394651413 | 31.6756844520569 | 17.8201140487299 | 178.547751903534 | 52.859437465667696 | 31.703394651413 | 106.6756844520569 | |
17.8273140948102 | 28.5314381122589 | 27.8717935085297 | 31.6694557666779 | 31.5467476844788 | 17.8273140948102 | 178.5314381122589 | 52.8717935085297 | 31.6694557666779 | 106.5467476844788 | |
17.8345141408905 | 28.515237569809 | 27.8784573078156 | 31.6110134124756 | 31.4221262931824 | 17.8345141408905 | 178.515237569809 | 52.8784573078156 | 31.6110134124756 | 106.4221262931824 | |
17.8417141869708 | 28.4997224807739 | 27.8784453868866 | 31.529837846756 | 31.3050746917725 | 17.8417141869708 | 178.4997224807739 | 52.8784453868866 | 31.529837846756 | 106.3050746917725 | |
17.8489142330511 | 28.4852147102356 | 27.8714179992676 | 31.4288437366486 | 31.1983704566956 | 17.8489142330511 | 178.4852147102356 | 52.8714179992676 | 31.4288437366486 | 106.1983704566956 | |
17.8561142791314 | 28.4719049930573 | 27.8575658798218 | 31.3119649887085 | 31.1041057109833 | 17.8561142791314 | 178.4719049930573 | 52.8575658798218 | 31.3119649887085 | 106.1041057109833 | |
17.8633143252117 | 28.4600198268891 | 27.8373122215271 | 31.1837911605835 | 31.0234308242798 | 17.8633143252117 | 178.4600198268891 | 52.8373122215271 | 31.1837911605835 | 106.0234308242798 | |
17.870514371292 | 28.4498155117035 | 27.8112769126892 | 31.0491800308228 | 30.9565722942353 | 17.870514371292 | 178.4498155117035 | 52.811276912689195 | 31.0491800308228 | 105.9565722942353 | |
17.8777144173723 | 28.4413874149323 | 27.7805387973786 | 30.9129416942597 | 30.9030532836914 | 17.8777144173723 | 178.4413874149323 | 52.7805387973786 | 30.9129416942597 | 105.9030532836914 | |
17.8849144634526 | 28.4345507621765 | 27.746844291687 | 30.7796120643616 | 30.861908197403 | 17.8849144634526 | 178.4345507621765 | 52.746844291687 | 30.7796120643616 | 105.861908197403 | |
17.8921145095329 | 28.4289002418518 | 27.7121961116791 | 30.6531012058258 | 30.8316111564636 | 17.8921145095329 | 178.4289002418518 | 52.7121961116791 | 30.6531012058258 | 105.8316111564636 | |
17.8993145556132 | 28.4240603446961 | 27.6781857013703 | 30.536299943924 | 30.8101534843445 | 17.8993145556132 | 178.4240603446961 | 52.678185701370296 | 30.536299943924 | 105.8101534843445 | |
17.9065146016935 | 28.4197628498078 | 27.6456892490387 | 30.4307818412781 | 30.795168876648 | 17.9065146016935 | 178.4197628498078 | 52.645689249038696 | 30.4307818412781 | 105.795168876648 | |
17.9137146477738 | 28.415858745575 | 27.6150941848755 | 30.3368866443634 | 30.7842195034027 | 17.9137146477738 | 178.415858745575 | 52.6150941848755 | 30.3368866443634 | 105.7842195034027 | |
17.9209146938541 | 28.4123241901398 | 27.5868773460388 | 30.2541732788086 | 30.7750165462494 | 17.9209146938541 | 178.4123241901398 | 52.586877346038804 | 30.2541732788086 | 105.7750165462494 | |
17.9281147399344 | 28.4093320369721 | 27.5617420673371 | 30.1817893981934 | 30.7655274868012 | 17.9281147399344 | 178.4093320369721 | 52.5617420673371 | 30.1817893981934 | 105.7655274868012 | |
17.9353147860147 | 28.4073352813721 | 27.5401592254639 | 30.1187038421631 | 30.754154920578 | 17.9353147860147 | 178.4073352813721 | 52.540159225463896 | 30.1187038421631 | 105.754154920578 | |
17.9425148320949 | 28.4069955348969 | 27.5221168994904 | 30.0637423992157 | 30.7399332523346 | 17.9425148320949 | 178.4069955348969 | 52.5221168994904 | 30.0637423992157 | 105.7399332523346 | |
17.9497148781752 | 28.4089684486389 | 27.5072157382965 | 30.0157248973847 | 30.7226538658142 | 17.9497148781752 | 178.4089684486389 | 52.5072157382965 | 30.0157248973847 | 105.7226538658142 | |
17.9569149242555 | 28.4137547016144 | 27.4951219558716 | 29.9737811088562 | 30.7026565074921 | 17.9569149242555 | 178.4137547016144 | 52.495121955871596 | 29.9737811088562 | 105.7026565074921 | |
17.9641149703358 | 28.4215986728668 | 27.4857938289643 | 29.9374461174011 | 30.6806147098541 | 17.9641149703358 | 178.4215986728668 | 52.485793828964304 | 29.9374461174011 | 105.6806147098541 | |
17.9713150164161 | 28.4323990345001 | 27.47922539711 | 29.9066245555878 | 30.6573212146759 | 17.9713150164161 | 178.4323990345001 | 52.47922539711 | 29.9066245555878 | 105.6573212146759 | |
17.9785150624964 | 28.4456312656403 | 27.4750471115113 | 29.8811793327332 | 30.6336641311646 | 17.9785150624964 | 178.44563126564032 | 52.4750471115113 | 29.8811793327332 | 105.63366413116461 |