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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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16.5457058925177 | 27.8325080871582 | 26.9292294979096 | 28.9313554763794 | 29.3641209602356 | 16.5457058925177 | 177.8325080871582 | 51.9292294979096 | 28.9313554763794 | 104.3641209602356 | |
16.552905938598 | 27.8724610805512 | 26.9403040409088 | 28.9229154586792 | 29.3611705303192 | 16.552905938598 | 177.8724610805512 | 51.9403040409088 | 28.9229154586792 | 104.3611705303192 | |
16.5601059846783 | 27.9162883758545 | 26.9510567188263 | 28.9139211177826 | 29.3590486049652 | 16.5601059846783 | 177.9162883758545 | 51.9510567188263 | 28.9139211177826 | 104.3590486049652 | |
16.5673060307586 | 27.9641211032868 | 26.9604206085205 | 28.9050757884979 | 29.357522726059 | 16.5673060307586 | 177.9641211032868 | 51.9604206085205 | 28.9050757884979 | 104.357522726059 | |
16.5745060768389 | 28.0161321163178 | 26.9676983356476 | 28.8971304893494 | 29.3563425540924 | 16.5745060768389 | 178.0161321163178 | 51.9676983356476 | 28.8971304893494 | 104.35634255409241 | |
16.5817061229192 | 28.0727446079254 | 26.9729435443878 | 28.8906753063202 | 29.355138540268 | 16.5817061229192 | 178.07274460792542 | 51.9729435443878 | 28.8906753063202 | 104.355138540268 | |
16.5889061689995 | 28.1347453594208 | 26.9769489765167 | 28.8860619068146 | 29.3536245822907 | 16.5889061689995 | 178.1347453594208 | 51.976948976516695 | 28.8860619068146 | 104.3536245822907 | |
16.5961062150798 | 28.2031655311585 | 26.9810497760773 | 28.8834035396576 | 29.3520033359528 | 16.5961062150798 | 178.2031655311585 | 51.9810497760773 | 28.8834035396576 | 104.3520033359528 | |
16.6033062611601 | 28.2791316509247 | 26.9865691661835 | 28.8826048374176 | 29.3510496616364 | 16.6033062611601 | 178.2791316509247 | 51.9865691661835 | 28.8826048374176 | 104.3510496616364 | |
16.6105063072404 | 28.3638238906861 | 26.9944429397583 | 28.8833796977997 | 29.3517351150513 | 16.6105063072404 | 178.3638238906861 | 51.9944429397583 | 28.8833796977997 | 104.3517351150513 | |
16.6177063533207 | 28.4585654735565 | 27.0052075386048 | 28.8853466510773 | 29.3548822402954 | 16.6177063533207 | 178.4585654735565 | 52.0052075386048 | 28.8853466510773 | 104.3548822402954 | |
16.624906399401 | 28.5647392272949 | 27.019077539444 | 28.8881063461304 | 29.3610036373139 | 16.624906399401 | 178.5647392272949 | 52.019077539444 | 28.8881063461304 | 104.3610036373139 | |
16.6321064454813 | 28.6835074424744 | 27.0360350608826 | 28.891259431839 | 29.3703854084015 | 16.6321064454813 | 178.6835074424744 | 52.0360350608826 | 28.891259431839 | 104.3703854084015 | |
16.6393064915616 | 28.8155496120453 | 27.0557284355164 | 28.8944721221924 | 29.383134841919 | 16.6393064915616 | 178.8155496120453 | 52.0557284355164 | 28.8944721221924 | 104.383134841919 | |
16.6465065376419 | 28.9612174034119 | 27.0775139331818 | 28.8974940776825 | 29.3991446495056 | 16.6465065376419 | 178.9612174034119 | 52.077513933181805 | 28.8974940776825 | 104.3991446495056 | |
16.6537065837222 | 29.120934009552 | 27.1005630493164 | 28.9002478122711 | 29.4180810451508 | 16.6537065837222 | 179.120934009552 | 52.1005630493164 | 28.9002478122711 | 104.4180810451508 | |
16.6609066298025 | 29.2956113815308 | 27.1243095397949 | 28.9028525352478 | 29.4393241405487 | 16.6609066298025 | 179.2956113815308 | 52.1243095397949 | 28.9028525352478 | 104.4393241405487 | |
16.6681066758827 | 29.4867992401123 | 27.1487832069397 | 28.9054811000824 | 29.4621586799622 | 16.6681066758827 | 179.4867992401123 | 52.1487832069397 | 28.9054811000824 | 104.4621586799622 | |
16.675306721963 | 29.6966850757599 | 27.174574136734 | 28.9082765579224 | 29.4859826564789 | 16.675306721963 | 179.6966850757599 | 52.174574136734 | 28.9082765579224 | 104.4859826564789 | |
16.6825067680433 | 29.9282968044281 | 27.2026538848877 | 28.9115428924561 | 29.5106291770935 | 16.6825067680433 | 179.9282968044281 | 52.202653884887695 | 28.9115428924561 | 104.5106291770935 | |
16.6897068141236 | 30.1859378814697 | 27.2339880466461 | 28.9158761501312 | 29.5365571975708 | 16.6897068141236 | 180.1859378814697 | 52.233988046646104 | 28.9158761501312 | 104.5365571975708 | |
16.6969068602039 | 30.4755508899689 | 27.2694170475006 | 28.9221942424774 | 29.5647621154785 | 16.6969068602039 | 180.4755508899689 | 52.269417047500596 | 28.9221942424774 | 104.5647621154785 | |
16.7041069062842 | 30.8050930500031 | 27.3096680641175 | 28.931474685669 | 29.5964300632477 | 16.7041069062842 | 180.8050930500031 | 52.3096680641175 | 28.931474685669 | 104.5964300632477 | |
16.7113069523645 | 31.1851024627686 | 27.3554503917694 | 28.9444208145142 | 29.6326637268067 | 16.7113069523645 | 181.1851024627686 | 52.355450391769395 | 28.9444208145142 | 104.6326637268067 | |
16.7185069984448 | 31.6295087337494 | 27.4072706699372 | 28.9613366127014 | 29.6745300292969 | 16.7185069984448 | 181.6295087337494 | 52.407270669937205 | 28.9613366127014 | 104.6745300292969 | |
16.7257070445251 | 32.1564137935639 | 27.465307712555 | 28.9820551872254 | 29.7232866287232 | 16.7257070445251 | 182.1564137935639 | 52.465307712555 | 28.9820551872254 | 104.7232866287232 | |
16.7329070906054 | 32.7885746955872 | 27.5294601917267 | 29.0059685707092 | 29.7805190086365 | 16.7329070906054 | 182.78857469558722 | 52.5294601917267 | 29.0059685707092 | 104.7805190086365 | |
16.7401071366857 | 33.5534691810608 | 27.5996625423432 | 29.0319085121155 | 29.848051071167 | 16.7401071366857 | 183.5534691810608 | 52.5996625423432 | 29.0319085121155 | 104.84805107116699 | |
16.747307182766 | 34.483277797699 | 27.6763021945954 | 29.0582597255707 | 29.927808046341 | 16.747307182766 | 184.483277797699 | 52.6763021945954 | 29.0582597255707 | 104.927808046341 | |
16.7545072288463 | 35.6151223182678 | 27.7603209018707 | 29.0834724903107 | 30.02188205719 | 16.7545072288463 | 185.6151223182678 | 52.7603209018707 | 29.0834724903107 | 105.02188205719 | |
16.7617072749266 | 36.9909942150116 | 27.8529107570648 | 29.1065990924835 | 30.1327526569367 | 16.7617072749266 | 186.9909942150116 | 52.852910757064805 | 29.1065990924835 | 105.1327526569367 | |
16.7689073210069 | 38.657283782959 | 27.9552042484284 | 29.1275560855866 | 30.2633464336395 | 16.7689073210069 | 188.65728378295898 | 52.9552042484284 | 29.1275560855866 | 105.2633464336395 | |
16.7761073670872 | 40.6635522842407 | 28.0681490898133 | 29.1470646858216 | 30.4167866706848 | 16.7761073670872 | 190.6635522842407 | 53.0681490898133 | 29.1470646858216 | 105.4167866706848 | |
16.7833074131675 | 43.0610716342926 | 28.1925916671753 | 29.166454076767 | 30.5960059165955 | 16.7833074131675 | 193.0610716342926 | 53.1925916671753 | 29.166454076767 | 105.5960059165955 | |
16.7905074592478 | 45.901083946228 | 28.3293843269348 | 29.1875898838043 | 30.8034658432007 | 16.7905074592478 | 195.901083946228 | 53.3293843269348 | 29.1875898838043 | 105.8034658432007 | |
16.7977075053281 | 49.2326855659485 | 28.4791946411133 | 29.2129158973694 | 31.0409665107727 | 16.7977075053281 | 199.2326855659485 | 53.479194641113295 | 29.2129158973694 | 106.0409665107727 | |
16.8049075514084 | 53.1001269817353 | 28.6424279212952 | 29.2451322078705 | 31.3096523284912 | 16.8049075514084 | 203.1001269817353 | 53.6424279212952 | 29.2451322078705 | 106.3096523284912 | |
16.8121075974886 | 57.5394749641419 | 28.8193166255951 | 29.2867541313172 | 31.6099345684052 | 16.8121075974886 | 207.5394749641419 | 53.8193166255951 | 29.2867541313172 | 106.60993456840521 | |
16.8193076435689 | 62.5749588012696 | 29.0102660655976 | 29.3397188186646 | 31.9413900375366 | 16.8193076435689 | 212.5749588012696 | 54.010266065597605 | 29.3397188186646 | 106.94139003753659 | |
16.8265076896492 | 68.2156026363373 | 29.2160570621491 | 29.4054031372071 | 32.3028385639191 | 16.8265076896492 | 218.21560263633728 | 54.216057062149105 | 29.4054031372071 | 107.3028385639191 | |
16.8337077357295 | 74.4522690773011 | 29.4377744197846 | 29.484885931015 | 32.6923727989197 | 16.8337077357295 | 224.45226907730108 | 54.4377744197846 | 29.484885931015 | 107.6923727989197 | |
16.8409077818098 | 81.2556862831116 | 29.6765148639679 | 29.5792520046234 | 33.1075668334961 | 16.8409077818098 | 231.2556862831116 | 54.676514863967896 | 29.5792520046234 | 108.1075668334961 | |
16.8481078278901 | 88.5759770870209 | 29.9330830574036 | 29.6895921230316 | 33.5455417633057 | 16.8481078278901 | 238.5759770870209 | 54.9330830574036 | 29.6895921230316 | 108.54554176330569 | |
16.8553078739704 | 96.3432729244232 | 30.2078545093537 | 29.8169612884522 | 34.0027749538422 | 16.8553078739704 | 246.34327292442322 | 55.2078545093537 | 29.8169612884522 | 109.00277495384219 | |
16.8625079200507 | 104.469394683838 | 30.5008828639984 | 29.9623310565949 | 34.4748198986054 | 16.8625079200507 | 254.469394683838 | 55.5008828639984 | 29.9623310565949 | 109.4748198986054 | |
16.869707966131 | 112.850052118302 | 30.8119535446167 | 30.1266074180603 | 34.9561750888825 | 16.869707966131 | 262.850052118302 | 55.8119535446167 | 30.1266074180603 | 109.9561750888825 | |
16.8769080122113 | 121.36754989624 | 31.1407685279846 | 30.3105354309082 | 35.4404807090759 | 16.8769080122113 | 271.36754989624 | 56.140768527984605 | 30.3105354309082 | 110.4404807090759 | |
16.8841080582916 | 129.89491224289 | 31.4869105815888 | 30.5145621299744 | 35.920512676239 | 16.8841080582916 | 279.89491224289 | 56.4869105815888 | 30.5145621299744 | 110.920512676239 | |
16.8913081043719 | 138.300722837448 | 31.8499445915222 | 30.7386875152588 | 36.3882780075073 | 16.8913081043719 | 288.300722837448 | 56.8499445915222 | 30.7386875152588 | 111.3882780075073 | |
16.8985081504522 | 146.453642845154 | 32.2295308113098 | 30.9822380542755 | 36.8349969387055 | 16.8985081504522 | 296.45364284515404 | 57.2295308113098 | 30.9822380542755 | 111.8349969387055 | |
16.9057081965325 | 154.225701093674 | 32.6255679130554 | 31.2437474727631 | 37.2514486312866 | 16.9057081965325 | 304.225701093674 | 57.6255679130554 | 31.2437474727631 | 112.25144863128659 | |
16.9129082426128 | 161.494219303131 | 33.0382525920868 | 31.5208077430725 | 37.6285016536713 | 16.9129082426128 | 311.494219303131 | 58.0382525920868 | 31.5208077430725 | 112.6285016536713 | |
16.9201082886931 | 168.143373727799 | 33.4678173065186 | 31.8100988864899 | 37.9576086997986 | 16.9201082886931 | 318.14337372779903 | 58.4678173065186 | 31.8100988864899 | 112.9576086997986 | |
16.9273083347734 | 174.067062139511 | 33.914053440094 | 32.107424736023 | 38.2310867309571 | 16.9273083347734 | 324.067062139511 | 58.914053440094 | 32.107424736023 | 113.2310867309571 | |
16.9345083808537 | 179.172283411026 | 34.3757808208466 | 32.4077785015106 | 38.4422838687897 | 16.9345083808537 | 329.172283411026 | 59.3757808208466 | 32.4077785015106 | 113.4422838687897 | |
16.941708426934 | 183.381599187851 | 34.8507583141327 | 32.7054798603058 | 38.5858774185181 | 16.941708426934 | 333.381599187851 | 59.8507583141327 | 32.7054798603058 | 113.5858774185181 | |
16.9489084730143 | 186.633962392807 | 35.3357017040253 | 32.9943537712097 | 38.6582493782044 | 16.9489084730143 | 336.633962392807 | 60.3357017040253 | 32.9943537712097 | 113.6582493782044 | |
16.9561085190945 | 188.884270191193 | 35.8265459537506 | 33.2680761814118 | 38.6577069759369 | 16.9561085190945 | 338.88427019119297 | 60.8265459537506 | 33.2680761814118 | 113.65770697593689 | |
16.9633085651748 | 190.10369181633 | 36.3184988498688 | 33.5206389427185 | 38.5845124721527 | 16.9633085651748 | 340.10369181633 | 61.3184988498688 | 33.5206389427185 | 113.58451247215271 | |
16.9705086112551 | 190.281593799591 | 36.8060767650604 | 33.7466180324555 | 38.44074010849 | 16.9705086112551 | 340.281593799591 | 61.8060767650604 | 33.7466180324555 | 113.44074010848999 | |
16.9777086573354 | 189.427506923676 | 37.2832655906677 | 33.9413464069367 | 38.2301986217499 | 16.9777086573354 | 339.427506923676 | 62.2832655906677 | 33.9413464069367 | 113.2301986217499 | |
16.9849087034157 | 187.570828199387 | 37.7439141273499 | 34.1009736061096 | 37.9584491252899 | 16.9849087034157 | 337.570828199387 | 62.7439141273499 | 34.1009736061096 | 112.9584491252899 | |
16.992108749496 | 184.758293628693 | 38.182145357132 | 34.2224001884461 | 37.6325190067291 | 16.992108749496 | 334.75829362869297 | 63.182145357132 | 34.2224001884461 | 112.6325190067291 | |
16.9993087955763 | 181.050848960877 | 38.592541217804 | 34.3032777309418 | 37.2605502605438 | 16.9993087955763 | 331.05084896087703 | 63.592541217804 | 34.3032777309418 | 112.2605502605438 | |
17.0065088416566 | 176.521903276444 | 38.9700710773468 | 34.3420803546906 | 36.8512928485871 | 17.0065088416566 | 326.521903276444 | 63.9700710773468 | 34.3420803546906 | 111.8512928485871 | |
17.0137088877369 | 171.256881952286 | 39.3094718456269 | 34.3380749225617 | 36.4136934280396 | 17.0137088877369 | 321.256881952286 | 64.3094718456269 | 34.3380749225617 | 111.41369342803961 | |
17.0209089338172 | 165.351855754853 | 39.6049499511719 | 34.2914998531342 | 35.9566569328308 | 17.0209089338172 | 315.351855754853 | 64.6049499511719 | 34.2914998531342 | 110.95665693283081 | |
17.0281089798975 | 158.910065889359 | 39.8503363132477 | 34.2037677764893 | 35.4887545108795 | 17.0281089798975 | 308.910065889359 | 64.85033631324771 | 34.2037677764893 | 110.4887545108795 | |
17.0353090259778 | 152.037835121155 | 40.0397479534149 | 34.0774595737457 | 35.0179314613342 | 17.0353090259778 | 302.037835121155 | 65.0397479534149 | 34.0774595737457 | 110.0179314613342 | |
17.0425090720581 | 144.841504096985 | 40.1683211326599 | 33.91632437706 | 34.5512628555298 | 17.0425090720581 | 294.841504096985 | 65.1683211326599 | 33.91632437706 | 109.5512628555298 | |
17.0497091181384 | 137.426149845124 | 40.2323722839356 | 33.7250590324402 | 34.0949594974518 | 17.0497091181384 | 287.426149845124 | 65.2323722839356 | 33.7250590324402 | 109.09495949745181 | |
17.0569091642187 | 129.894691705704 | 40.2292847633362 | 33.5090041160584 | 33.6544632911682 | 17.0569091642187 | 279.89469170570396 | 65.22928476333621 | 33.5090041160584 | 108.6544632911682 | |
17.064109210299 | 122.346311807633 | 40.1577115058899 | 33.273708820343 | 33.2345426082611 | 17.064109210299 | 272.346311807633 | 65.15771150588989 | 33.273708820343 | 108.23454260826111 | |
17.0713092563793 | 114.873969554901 | 40.0181710720062 | 33.024549484253 | 32.8393042087555 | 17.0713092563793 | 264.873969554901 | 65.0181710720062 | 33.024549484253 | 107.8393042087555 | |
17.0785093024596 | 107.562160491944 | 39.813494682312 | 32.7663362026215 | 32.4719965457916 | 17.0785093024596 | 257.562160491944 | 64.813494682312 | 32.7663362026215 | 107.4719965457916 | |
17.0857093485399 | 100.485414266587 | 39.548534154892 | 32.5031876564026 | 32.1349918842316 | 17.0857093485399 | 250.485414266587 | 64.548534154892 | 32.5031876564026 | 107.1349918842316 | |
17.0929093946201 | 93.707424402237 | 39.2291069030762 | 32.2386741638184 | 31.8297624588013 | 17.0929093946201 | 243.707424402237 | 64.2291069030762 | 32.2386741638184 | 106.8297624588013 | |
17.1001094407004 | 87.2803092002869 | 38.8609766960144 | 31.9760143756867 | 31.5569818019867 | 17.1001094407004 | 237.2803092002869 | 63.8609766960144 | 31.9760143756867 | 106.5569818019867 | |
17.1073094867807 | 81.2441527843476 | 38.449776172638 | 31.7182898521424 | 31.3164532184601 | 17.1073094867807 | 231.2441527843476 | 63.449776172638 | 31.7182898521424 | 106.3164532184601 | |
17.114509532861 | 75.6267070770264 | 38.0013465881348 | 31.4685046672821 | 31.1069428920746 | 17.114509532861 | 225.6267070770264 | 63.0013465881348 | 31.4685046672821 | 106.1069428920746 | |
17.1217095789413 | 70.443481206894 | 37.5219106674195 | 31.2293946743012 | 30.9262871742249 | 17.1217095789413 | 220.443481206894 | 62.5219106674195 | 31.2293946743012 | 105.9262871742249 | |
17.1289096250216 | 65.6986594200135 | 37.0177388191223 | 31.0033023357392 | 30.7717204093933 | 17.1289096250216 | 215.69865942001348 | 62.0177388191223 | 31.0033023357392 | 105.7717204093933 | |
17.1361096711019 | 61.386638879776 | 36.4946603775025 | 30.7920932769776 | 30.640321969986 | 17.1361096711019 | 211.386638879776 | 61.4946603775025 | 30.7920932769776 | 105.640321969986 | |
17.1433097171822 | 57.494193315506 | 35.9579682350159 | 30.5969476699829 | 30.52938580513 | 17.1433097171822 | 207.494193315506 | 60.9579682350159 | 30.5969476699829 | 105.52938580513 | |
17.1505097632625 | 54.0027916431427 | 35.4128241539002 | 30.418336391449 | 30.4365277290344 | 17.1505097632625 | 204.0027916431427 | 60.4128241539002 | 30.418336391449 | 105.4365277290344 | |
17.1577098093428 | 50.8901536464691 | 34.864491224289 | 30.256062746048 | 30.3596615791321 | 17.1577098093428 | 200.89015364646912 | 59.864491224289 | 30.256062746048 | 105.35966157913211 | |
17.1649098554231 | 48.1313228607178 | 34.3181967735291 | 30.1095902919769 | 30.2970230579376 | 17.1649098554231 | 198.1313228607178 | 59.3181967735291 | 30.1095902919769 | 105.2970230579376 | |
17.1721099015034 | 45.6995308399201 | 33.7789356708527 | 29.9784600734711 | 30.2471935749054 | 17.1721099015034 | 195.6995308399201 | 58.7789356708527 | 29.9784600734711 | 105.2471935749054 | |
17.1793099475837 | 43.5669660568238 | 33.2513153553009 | 29.8625111579895 | 30.208969116211 | 17.1793099475837 | 193.5669660568238 | 58.2513153553009 | 29.8625111579895 | 105.208969116211 | |
17.186509993664 | 41.7056858539582 | 32.7396810054779 | 29.7619640827179 | 30.1811158657074 | 17.186509993664 | 191.7056858539582 | 57.7396810054779 | 29.7619640827179 | 105.1811158657074 | |
17.1937100397443 | 40.0881111621857 | 32.2482168674469 | 29.6772003173828 | 30.1620960235596 | 17.1937100397443 | 190.0881111621857 | 57.2482168674469 | 29.6772003173828 | 105.1620960235596 | |
17.2009100858246 | 38.6873066425324 | 31.7806839942932 | 29.6086311340332 | 30.1500797271729 | 17.2009100858246 | 188.6873066425324 | 56.7806839942932 | 29.6086311340332 | 105.15007972717291 | |
17.2081101319049 | 37.4771118164063 | 31.3401877880097 | 29.5563936233521 | 30.1430761814118 | 17.2081101319049 | 187.4771118164063 | 56.3401877880097 | 29.5563936233521 | 105.1430761814118 | |
17.2153101779852 | 36.4326000213623 | 30.9291064739228 | 29.5201063156128 | 30.1391541957855 | 17.2153101779852 | 186.4326000213623 | 55.9291064739228 | 29.5201063156128 | 105.1391541957855 | |
17.2225102240655 | 35.5306565761566 | 30.5491626262665 | 29.4987380504608 | 30.1366090774536 | 17.2225102240655 | 185.53065657615662 | 55.5491626262665 | 29.4987380504608 | 105.1366090774536 | |
17.2297102701458 | 34.7505331039429 | 30.2013695240021 | 29.4906198978424 | 30.1339685916901 | 17.2297102701458 | 184.7505331039429 | 55.201369524002104 | 29.4906198978424 | 105.13396859169009 | |
17.236910316226 | 34.0741753578186 | 29.8858761787415 | 29.4937789440155 | 30.1300883293152 | 17.236910316226 | 184.0741753578186 | 54.8858761787415 | 29.4937789440155 | 105.1300883293152 | |
17.2441103623063 | 33.4861516952515 | 29.6017706394196 | 29.5061409473419 | 30.1242113113404 | 17.2441103623063 | 183.4861516952515 | 54.6017706394196 | 29.5061409473419 | 105.1242113113404 | |
17.2513104083866 | 32.9735457897186 | 29.34730052948 | 29.5257449150086 | 30.1159381866455 | 17.2513104083866 | 182.9735457897186 | 54.347300529479995 | 29.5257449150086 | 105.11593818664551 | |
17.2585104544669 | 32.5256049633026 | 29.120421409607 | 29.550713300705 | 30.1051080226898 | 17.2585104544669 | 182.5256049633026 | 54.120421409607005 | 29.550713300705 | 105.1051080226898 |