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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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15.8257012844882 | 27.741551399231 | 27.028238773346 | 29.4224917888642 | 29.5966565608978 | 15.8257012844882 | 177.741551399231 | 52.028238773346004 | 29.4224917888642 | 104.5966565608978 | |
15.8329013305685 | 27.7578115463257 | 27.0242273807526 | 29.3746709823609 | 29.5481383800507 | 15.8329013305685 | 177.7578115463257 | 52.0242273807526 | 29.3746709823609 | 104.5481383800507 | |
15.8401013766488 | 27.7702510356903 | 27.0212769508362 | 29.3251216411591 | 29.4982790946961 | 15.8401013766488 | 177.7702510356903 | 52.021276950836196 | 29.3251216411591 | 104.4982790946961 | |
15.8473014227291 | 27.7790546417237 | 27.0197033882141 | 29.2758584022522 | 29.4492781162262 | 15.8473014227291 | 177.7790546417237 | 52.0197033882141 | 29.2758584022522 | 104.4492781162262 | |
15.8545014688094 | 27.7840971946717 | 27.0197331905365 | 29.2289674282074 | 29.4038593769074 | 15.8545014688094 | 177.7840971946717 | 52.0197331905365 | 29.2289674282074 | 104.4038593769074 | |
15.8617015148897 | 27.785074710846 | 27.0213425159455 | 29.1862010955811 | 29.364562034607 | 15.8617015148897 | 177.785074710846 | 52.0213425159455 | 29.1862010955811 | 104.364562034607 | |
15.86890156097 | 27.7819633483887 | 27.0242154598236 | 29.148405790329 | 29.3329536914826 | 15.86890156097 | 177.7819633483887 | 52.0242154598236 | 29.148405790329 | 104.3329536914826 | |
15.8761016070503 | 27.7751684188843 | 27.0278155803681 | 29.1153013706207 | 29.309493303299 | 15.8761016070503 | 177.7751684188843 | 52.0278155803681 | 29.1153013706207 | 104.309493303299 | |
15.8833016531306 | 27.7653396129608 | 27.0317077636719 | 29.0858328342438 | 29.293966293335 | 15.8833016531306 | 177.76533961296082 | 52.0317077636719 | 29.0858328342438 | 104.293966293335 | |
15.8905016992109 | 27.753084897995 | 27.0356357097626 | 29.0588974952698 | 29.2858958244324 | 15.8905016992109 | 177.753084897995 | 52.0356357097626 | 29.0588974952698 | 104.2858958244324 | |
15.8977017452912 | 27.7388453483582 | 27.0393550395966 | 29.0338575839997 | 29.2846143245697 | 15.8977017452912 | 177.7388453483582 | 52.0393550395966 | 29.0338575839997 | 104.2846143245697 | |
15.9049017913715 | 27.7231931686402 | 27.0422995090485 | 29.0106117725373 | 29.2890846729279 | 15.9049017913715 | 177.7231931686402 | 52.042299509048505 | 29.0106117725373 | 104.2890846729279 | |
15.9121018374518 | 27.7069926261902 | 27.0435810089112 | 28.9893865585327 | 29.2979836463928 | 15.9121018374518 | 177.70699262619019 | 52.043581008911204 | 28.9893865585327 | 104.2979836463928 | |
15.9193018835321 | 27.6911556720734 | 27.0422697067261 | 28.9706707000733 | 29.3102264404297 | 15.9193018835321 | 177.6911556720734 | 52.0422697067261 | 28.9706707000733 | 104.3102264404297 | |
15.9265019296124 | 27.6761174201966 | 27.0378530025482 | 28.9552986621857 | 29.3253004550934 | 15.9265019296124 | 177.6761174201966 | 52.037853002548204 | 28.9552986621857 | 104.3253004550934 | |
15.9337019756927 | 27.661544084549 | 27.0302176475525 | 28.9442896842957 | 29.3431222438812 | 15.9337019756927 | 177.661544084549 | 52.030217647552504 | 28.9442896842957 | 104.3431222438812 | |
15.940902021773 | 27.6466369628906 | 27.0194888114929 | 28.9384603500366 | 29.3637156486511 | 15.940902021773 | 177.6466369628906 | 52.0194888114929 | 28.9384603500366 | 104.3637156486511 | |
15.9481020678533 | 27.6307344436646 | 27.0059108734131 | 28.9380192756653 | 29.3869733810425 | 15.9481020678533 | 177.6307344436646 | 52.0059108734131 | 28.9380192756653 | 104.3869733810425 | |
15.9553021139335 | 27.6134967803955 | 26.9899725914002 | 28.942483663559 | 29.4127285480499 | 15.9553021139335 | 177.6134967803955 | 51.9899725914002 | 28.942483663559 | 104.4127285480499 | |
15.9625021600138 | 27.5947093963623 | 26.9725441932678 | 28.9510190486908 | 29.4409155845642 | 15.9625021600138 | 177.5947093963623 | 51.9725441932678 | 28.9510190486908 | 104.4409155845642 | |
15.9697022060941 | 27.5740325450897 | 26.9546866416931 | 28.9629340171814 | 29.4715344905853 | 15.9697022060941 | 177.5740325450897 | 51.9546866416931 | 28.9629340171814 | 104.4715344905853 | |
15.9769022521744 | 27.551132440567 | 26.9372999668122 | 28.9777755737305 | 29.5042395591736 | 15.9769022521744 | 177.551132440567 | 51.937299966812205 | 28.9777755737305 | 104.5042395591736 | |
15.9841022982547 | 27.526205778122 | 26.9207835197449 | 28.9952218532562 | 29.5381903648377 | 15.9841022982547 | 177.526205778122 | 51.9207835197449 | 28.9952218532562 | 104.5381903648377 | |
15.991302344335 | 27.5001704692841 | 26.9052505493164 | 29.0148735046387 | 29.5719921588898 | 15.991302344335 | 177.50017046928411 | 51.9052505493164 | 29.0148735046387 | 104.5719921588898 | |
15.9985023904153 | 27.4742960929871 | 26.8908262252808 | 29.0363073348999 | 29.6038389205933 | 15.9985023904153 | 177.4742960929871 | 51.890826225280804 | 29.0363073348999 | 104.6038389205933 | |
16.0057024364956 | 27.4495601654053 | 26.8778204917908 | 29.0590822696686 | 29.6316623687744 | 16.0057024364956 | 177.4495601654053 | 51.8778204917908 | 29.0590822696686 | 104.6316623687744 | |
16.0129024825759 | 27.4264454841614 | 26.8665909767151 | 29.0826797485352 | 29.6534717082978 | 16.0129024825759 | 177.4264454841614 | 51.8665909767151 | 29.0826797485352 | 104.6534717082978 | |
16.0201025286562 | 27.4052083492279 | 26.8573522567749 | 29.1062891483307 | 29.6675264835358 | 16.0201025286562 | 177.4052083492279 | 51.8573522567749 | 29.1062891483307 | 104.6675264835358 | |
16.0273025747365 | 27.3862600326538 | 26.8502056598664 | 29.12877202034 | 29.6726822853089 | 16.0273025747365 | 177.3862600326538 | 51.850205659866404 | 29.12877202034 | 104.6726822853089 | |
16.0345026208168 | 27.3700594902039 | 26.8453061580658 | 29.1489183902741 | 29.6686708927155 | 16.0345026208168 | 177.37005949020391 | 51.845306158065796 | 29.1489183902741 | 104.6686708927155 | |
16.0417026668971 | 27.3567855358124 | 26.8429100513458 | 29.1657626628876 | 29.6563148498535 | 16.0417026668971 | 177.3567855358124 | 51.8429100513458 | 29.1657626628876 | 104.6563148498535 | |
16.0489027129774 | 27.346134185791 | 26.8432378768921 | 29.178786277771 | 29.6373903751374 | 16.0489027129774 | 177.34613418579102 | 51.843237876892104 | 29.178786277771 | 104.6373903751374 | |
16.0561027590577 | 27.3377418518067 | 26.8461465835571 | 29.1877925395966 | 29.6143770217896 | 16.0561027590577 | 177.3377418518067 | 51.8461465835571 | 29.1877925395966 | 104.61437702178961 | |
16.063302805138 | 27.3314416408539 | 26.8511474132538 | 29.1926860809326 | 29.5900404453278 | 16.063302805138 | 177.3314416408539 | 51.8511474132538 | 29.1926860809326 | 104.5900404453278 | |
16.0705028512183 | 27.3273289203644 | 26.8574953079224 | 29.1934788227082 | 29.5670449733734 | 16.0705028512183 | 177.3273289203644 | 51.8574953079224 | 29.1934788227082 | 104.5670449733734 | |
16.0777028972986 | 27.3253917694092 | 26.864492893219 | 29.1902661323548 | 29.5477747917176 | 16.0777028972986 | 177.3253917694092 | 51.864492893219 | 29.1902661323548 | 104.5477747917176 | |
16.0849029433789 | 27.3252129554749 | 26.8715798854828 | 29.1832029819489 | 29.5340657234192 | 16.0849029433789 | 177.3252129554749 | 51.8715798854828 | 29.1832029819489 | 104.5340657234192 | |
16.0921029894592 | 27.3260474205017 | 26.8783807754517 | 29.1724801063538 | 29.5270740985871 | 16.0921029894592 | 177.3260474205017 | 51.8783807754517 | 29.1724801063538 | 104.5270740985871 | |
16.0993030355395 | 27.3271977901459 | 26.8847346305847 | 29.1584610939026 | 29.5273005962372 | 16.0993030355395 | 177.3271977901459 | 51.8847346305847 | 29.1584610939026 | 104.5273005962372 | |
16.1065030816197 | 27.328109741211 | 26.8907427787781 | 29.1418433189392 | 29.5348644256592 | 16.1065030816197 | 177.328109741211 | 51.890742778778105 | 29.1418433189392 | 104.53486442565921 | |
16.1137031277 | 27.3283302783966 | 26.8967092037201 | 29.1238248348236 | 29.5497477054596 | 16.1137031277 | 177.3283302783966 | 51.8967092037201 | 29.1238248348236 | 104.5497477054596 | |
16.1209031737803 | 27.327424287796 | 26.9030869007111 | 29.106080532074 | 29.5717179775238 | 16.1209031737803 | 177.327424287796 | 51.9030869007111 | 29.106080532074 | 104.5717179775238 | |
16.1281032198606 | 27.3252308368683 | 26.9102871417999 | 29.0905117988587 | 29.6001613140106 | 16.1281032198606 | 177.3252308368683 | 51.9102871417999 | 29.0905117988587 | 104.60016131401059 | |
16.1353032659409 | 27.322119474411 | 26.9185602664948 | 29.0790498256684 | 29.6339571475983 | 16.1353032659409 | 177.322119474411 | 51.9185602664948 | 29.0790498256684 | 104.6339571475983 | |
16.1425033120212 | 27.3191213607788 | 26.9279778003693 | 29.0734767913819 | 29.6718895435333 | 16.1425033120212 | 177.3191213607788 | 51.927977800369305 | 29.0734767913819 | 104.6718895435333 | |
16.1497033581015 | 27.3175954818726 | 26.938396692276 | 29.075026512146 | 29.7129809856415 | 16.1497033581015 | 177.31759548187262 | 51.938396692276 | 29.075026512146 | 104.71298098564151 | |
16.1569034041818 | 27.3188531398773 | 26.9496023654938 | 29.0841400623322 | 29.7563791275025 | 16.1569034041818 | 177.3188531398773 | 51.9496023654938 | 29.0841400623322 | 104.7563791275025 | |
16.1641034502621 | 27.3239552974701 | 26.9612729549408 | 29.1001856327057 | 29.8010349273682 | 16.1641034502621 | 177.3239552974701 | 51.961272954940796 | 29.1001856327057 | 104.80103492736819 | |
16.1713034963424 | 27.3335874080658 | 26.9730269908905 | 29.121607542038 | 29.845654964447 | 16.1713034963424 | 177.3335874080658 | 51.9730269908905 | 29.121607542038 | 104.845654964447 | |
16.1785035424227 | 27.3478925228119 | 26.9843578338623 | 29.1463971138001 | 29.8890590667725 | 16.1785035424227 | 177.3478925228119 | 51.984357833862305 | 29.1463971138001 | 104.8890590667725 | |
16.185703588503 | 27.3663759231568 | 26.9946575164795 | 29.1726350784302 | 29.9306154251099 | 16.185703588503 | 177.3663759231568 | 51.9946575164795 | 29.1726350784302 | 104.93061542510989 | |
16.1929036345833 | 27.3877382278443 | 27.0032703876496 | 29.1988253593445 | 29.9701571464539 | 16.1929036345833 | 177.3877382278443 | 52.0032703876496 | 29.1988253593445 | 104.9701571464539 | |
16.2001036806636 | 27.4100661277771 | 27.0095348358155 | 29.224169254303 | 30.007404088974 | 16.2001036806636 | 177.4100661277771 | 52.0095348358155 | 29.224169254303 | 105.007404088974 | |
16.2073037267439 | 27.4312376976013 | 27.0127594470978 | 29.2486190795899 | 30.0415694713593 | 16.2073037267439 | 177.4312376976013 | 52.0127594470978 | 29.2486190795899 | 105.0415694713593 | |
16.2145037728242 | 27.4493873119354 | 27.0122468471527 | 29.2728662490845 | 30.0716042518616 | 16.2145037728242 | 177.4493873119354 | 52.012246847152696 | 29.2728662490845 | 105.0716042518616 | |
16.2217038189045 | 27.4632453918457 | 27.0075023174286 | 29.2981445789337 | 30.0966918468476 | 16.2217038189045 | 177.4632453918457 | 52.0075023174286 | 29.2981445789337 | 105.0966918468476 | |
16.2289038649848 | 27.4722278118134 | 26.9985556602478 | 29.3257236480713 | 30.1165521144867 | 16.2289038649848 | 177.4722278118134 | 51.9985556602478 | 29.3257236480713 | 105.1165521144867 | |
16.2361039110651 | 27.4763762950897 | 26.9862294197083 | 29.3563008308411 | 30.1312923431397 | 16.2361039110651 | 177.4763762950897 | 51.9862294197083 | 29.3563008308411 | 105.1312923431397 | |
16.2433039571453 | 27.4761140346527 | 26.9719243049622 | 29.3896079063416 | 30.1411151885987 | 16.2433039571453 | 177.4761140346527 | 51.9719243049622 | 29.3896079063416 | 105.1411151885987 | |
16.2505040032256 | 27.4722337722779 | 26.9572198390961 | 29.4244289398194 | 30.1462709903717 | 16.2505040032256 | 177.4722337722779 | 51.9572198390961 | 29.4244289398194 | 105.1462709903717 | |
16.2577040493059 | 27.4657964706421 | 26.9436061382294 | 29.4588565826416 | 30.1471829414368 | 16.2577040493059 | 177.4657964706421 | 51.9436061382294 | 29.4588565826416 | 105.1471829414368 | |
16.2649040953862 | 27.4580419063568 | 26.9324779510498 | 29.4906497001648 | 30.1444470882416 | 16.2649040953862 | 177.4580419063568 | 51.932477951049805 | 29.4906497001648 | 105.1444470882416 | |
16.2721041414665 | 27.4501144886017 | 26.9250392913819 | 29.517525434494 | 30.138623714447 | 16.2721041414665 | 177.45011448860168 | 51.9250392913819 | 29.517525434494 | 105.138623714447 | |
16.2793041875468 | 27.4428725242615 | 26.922070980072 | 29.5374870300293 | 30.1299631595612 | 16.2793041875468 | 177.4428725242615 | 51.922070980072 | 29.5374870300293 | 105.1299631595612 | |
16.2865042336271 | 27.4368286132813 | 26.9234478473664 | 29.5493125915528 | 30.1183104515076 | 16.2865042336271 | 177.4368286132813 | 51.923447847366404 | 29.5493125915528 | 105.1183104515076 | |
16.2937042797074 | 27.4322986602783 | 26.9279778003693 | 29.5528471469879 | 30.1031887531281 | 16.2937042797074 | 177.4322986602783 | 51.927977800369305 | 29.5528471469879 | 105.10318875312811 | |
16.3009043257877 | 27.4296522140503 | 26.9338071346283 | 29.5490026473999 | 30.0840377807617 | 16.3009043257877 | 177.4296522140503 | 51.933807134628296 | 29.5490026473999 | 105.0840377807617 | |
16.308104371868 | 27.4294316768646 | 26.9390523433686 | 29.5393824577332 | 30.060338973999 | 16.308104371868 | 177.4294316768646 | 51.9390523433686 | 29.5393824577332 | 105.060338973999 | |
16.3153044179483 | 27.4321794509888 | 26.9422233104706 | 29.5258641242981 | 30.0317883491516 | 16.3153044179483 | 177.4321794509888 | 51.942223310470595 | 29.5258641242981 | 105.0317883491516 | |
16.3225044640286 | 27.4382114410401 | 26.942378282547 | 29.5103251934052 | 29.9984574317932 | 16.3225044640286 | 177.4382114410401 | 51.942378282547 | 29.5103251934052 | 104.9984574317932 | |
16.3297045101089 | 27.4475395679474 | 26.9391477108002 | 29.4945061206818 | 29.9607038497925 | 16.3297045101089 | 177.4475395679474 | 51.9391477108002 | 29.4945061206818 | 104.9607038497925 | |
16.3369045561892 | 27.4598181247711 | 26.9328236579895 | 29.4797420501709 | 29.9192905426026 | 16.3369045561892 | 177.4598181247711 | 51.9328236579895 | 29.4797420501709 | 104.9192905426026 | |
16.3441046022695 | 27.4743437767029 | 26.9244849681854 | 29.4666886329651 | 29.8751950263977 | 16.3441046022695 | 177.4743437767029 | 51.924484968185396 | 29.4666886329651 | 104.8751950263977 | |
16.3513046483498 | 27.490234375 | 26.915854215622 | 29.4552147388458 | 29.8296511173249 | 16.3513046483498 | 177.490234375 | 51.915854215622005 | 29.4552147388458 | 104.8296511173249 | |
16.3585046944301 | 27.5063931941986 | 26.9089162349701 | 29.4445157051087 | 29.7840595245362 | 16.3585046944301 | 177.5063931941986 | 51.9089162349701 | 29.4445157051087 | 104.7840595245362 | |
16.3657047405104 | 27.5216460227967 | 26.9054591655731 | 29.4333696365357 | 29.7397494316101 | 16.3657047405104 | 177.5216460227967 | 51.9054591655731 | 29.4333696365357 | 104.73974943161011 | |
16.3729047865907 | 27.5348365306854 | 26.9067287445069 | 29.4204831123352 | 29.6978235244751 | 16.3729047865907 | 177.5348365306854 | 51.9067287445069 | 29.4204831123352 | 104.6978235244751 | |
16.380104832671 | 27.5449991226196 | 26.9131183624268 | 29.4047236442566 | 29.6590447425842 | 16.380104832671 | 177.5449991226196 | 51.9131183624268 | 29.4047236442566 | 104.6590447425842 | |
16.3873048787512 | 27.5515973567963 | 26.9241094589234 | 29.385370016098 | 29.6237826347351 | 16.3873048787512 | 177.5515973567963 | 51.9241094589234 | 29.385370016098 | 104.62378263473511 | |
16.3945049248315 | 27.5548458099365 | 26.9383609294892 | 29.3623208999634 | 29.5921206474304 | 16.3945049248315 | 177.5548458099365 | 51.9383609294892 | 29.3623208999634 | 104.59212064743039 | |
16.4017049709118 | 27.5556743144989 | 26.9539654254914 | 29.336029291153 | 29.5638799667359 | 16.4017049709118 | 177.5556743144989 | 51.953965425491404 | 29.336029291153 | 104.5638799667359 | |
16.4089050169921 | 27.5553643703461 | 26.9688427448273 | 29.3072879314423 | 29.5387625694275 | 16.4089050169921 | 177.5553643703461 | 51.9688427448273 | 29.3072879314423 | 104.5387625694275 | |
16.4161050630724 | 27.5551676750183 | 26.9809424877167 | 29.2770147323609 | 29.5166194438935 | 16.4161050630724 | 177.5551676750183 | 51.9809424877167 | 29.2770147323609 | 104.5166194438935 | |
16.4233051091527 | 27.5559902191162 | 26.9886732101441 | 29.2461395263672 | 29.4975340366364 | 16.4233051091527 | 177.5559902191162 | 51.9886732101441 | 29.2461395263672 | 104.4975340366364 | |
16.430505155233 | 27.5583982467652 | 26.9911646842957 | 29.2154908180237 | 29.4817268848419 | 16.430505155233 | 177.5583982467652 | 51.9911646842957 | 29.2154908180237 | 104.4817268848419 | |
16.4377052013133 | 27.5627136230469 | 26.9884705543518 | 29.1857302188874 | 29.4693470001221 | 16.4377052013133 | 177.5627136230469 | 51.9884705543518 | 29.1857302188874 | 104.4693470001221 | |
16.4449052473936 | 27.5690078735352 | 26.9814789295197 | 29.157155752182 | 29.4602632522583 | 16.4449052473936 | 177.5690078735352 | 51.981478929519696 | 29.157155752182 | 104.4602632522583 | |
16.4521052934739 | 27.5769591331482 | 26.9714832305908 | 29.129707813263 | 29.4540405273438 | 16.4521052934739 | 177.5769591331482 | 51.9714832305908 | 29.129707813263 | 104.4540405273438 | |
16.4593053395542 | 27.5859951972962 | 26.9598245620728 | 29.1031837463379 | 29.4499635696411 | 16.4593053395542 | 177.5859951972962 | 51.9598245620728 | 29.1031837463379 | 104.4499635696411 | |
16.4665053856345 | 27.5956273078919 | 26.9476652145386 | 29.077559709549 | 29.4470489025116 | 16.4665053856345 | 177.5956273078919 | 51.9476652145386 | 29.077559709549 | 104.4470489025116 | |
16.4737054317148 | 27.6057600975037 | 26.9359767436981 | 29.0531873703003 | 29.4440686702728 | 16.4737054317148 | 177.6057600975037 | 51.9359767436981 | 29.0531873703003 | 104.4440686702728 | |
16.4809054777951 | 27.616822719574 | 26.9254326820374 | 29.0307223796845 | 29.4398725032807 | 16.4809054777951 | 177.616822719574 | 51.925432682037396 | 29.0307223796845 | 104.4398725032807 | |
16.4881055238754 | 27.6295185089112 | 26.9164323806763 | 29.0109097957611 | 29.4337034225464 | 16.4881055238754 | 177.6295185089112 | 51.9164323806763 | 29.0109097957611 | 104.4337034225464 | |
16.4953055699557 | 27.644544839859 | 26.9091963768006 | 28.9941370487213 | 29.4254124164582 | 16.4953055699557 | 177.644544839859 | 51.9091963768006 | 28.9941370487213 | 104.4254124164582 | |
16.502505616036 | 27.6623368263245 | 26.9039392471314 | 28.9803981781006 | 29.415363073349 | 16.502505616036 | 177.6623368263245 | 51.903939247131404 | 28.9803981781006 | 104.415363073349 | |
16.5097056621163 | 27.6830852031708 | 26.9010484218598 | 28.969293832779 | 29.4042646884918 | 16.5097056621163 | 177.6830852031708 | 51.9010484218598 | 28.969293832779 | 104.40426468849179 | |
16.5169057081966 | 27.7068197727204 | 26.9010305404663 | 28.960257768631 | 29.3930768966675 | 16.5169057081966 | 177.7068197727204 | 51.9010305404663 | 28.960257768631 | 104.3930768966675 | |
16.5241057542768 | 27.7335464954376 | 26.9041359424591 | 28.9526522159577 | 29.382860660553 | 16.5241057542768 | 177.7335464954376 | 51.9041359424591 | 28.9526522159577 | 104.382860660553 | |
16.5313058003571 | 27.7633011341095 | 26.9102573394776 | 28.9457976818085 | 29.3745040893555 | 16.5313058003571 | 177.7633011341095 | 51.910257339477596 | 28.9457976818085 | 104.3745040893555 | |
16.5385058464374 | 27.79620885849 | 26.918888092041 | 28.9389073848725 | 29.3683648109436 | 16.5385058464374 | 177.79620885849 | 51.918888092041 | 28.9389073848725 | 104.3683648109436 |