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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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14.3856920684293 | 26.8890261650086 | 26.8268465995789 | 29.3609976768494 | 30.6579470634461 | 14.3856920684293 | 176.8890261650086 | 51.8268465995789 | 29.3609976768494 | 105.6579470634461 | |
14.3928921145096 | 26.8999457359314 | 26.8359959125519 | 29.4667661190033 | 30.9035956859589 | 14.3928921145096 | 176.8999457359314 | 51.8359959125519 | 29.4667661190033 | 105.9035956859589 | |
14.4000921605899 | 26.9137799739838 | 26.847106218338 | 29.5840263366699 | 31.1735570430756 | 14.4000921605899 | 176.9137799739838 | 51.847106218338 | 29.5840263366699 | 106.1735570430756 | |
14.4072922066701 | 26.9307851791382 | 26.8603146076203 | 29.7133445739746 | 31.4676702022553 | 14.4072922066701 | 176.9307851791382 | 51.860314607620296 | 29.7133445739746 | 106.4676702022553 | |
14.4144922527504 | 26.9507586956024 | 26.8754363059998 | 29.8560440540314 | 31.7843854427338 | 14.4144922527504 | 176.9507586956024 | 51.8754363059998 | 29.8560440540314 | 106.7843854427338 | |
14.4216922988307 | 26.9733726978302 | 26.8916845321655 | 30.0137519836426 | 32.1204483509064 | 14.4216922988307 | 176.9733726978302 | 51.8916845321655 | 30.0137519836426 | 107.1204483509064 | |
14.428892344911 | 26.9983649253845 | 26.9079566001892 | 30.1876604557038 | 32.4709177017212 | 14.428892344911 | 176.9983649253845 | 51.907956600189195 | 30.1876604557038 | 107.47091770172119 | |
14.4360923909913 | 27.0254790782929 | 26.9232630729676 | 30.3781151771546 | 32.8293025493622 | 14.4360923909913 | 177.0254790782929 | 51.9232630729676 | 30.3781151771546 | 107.8293025493622 | |
14.4432924370716 | 27.0542860031128 | 26.9368052482605 | 30.5846393108368 | 33.1879794597626 | 14.4432924370716 | 177.0542860031128 | 51.9368052482605 | 30.5846393108368 | 108.1879794597626 | |
14.4504924831519 | 27.0842790603638 | 26.9478380680084 | 30.8058977127075 | 33.5385859012604 | 14.4504924831519 | 177.0842790603638 | 51.9478380680084 | 30.8058977127075 | 108.5385859012604 | |
14.4576925292322 | 27.1150887012482 | 26.9555926322937 | 31.0395419597626 | 33.8724493980408 | 14.4576925292322 | 177.1150887012482 | 51.9555926322937 | 31.0395419597626 | 108.8724493980408 | |
14.4648925753125 | 27.1467030048371 | 26.9594788551331 | 31.2817394733429 | 34.1809153556824 | 14.4648925753125 | 177.1467030048371 | 51.9594788551331 | 31.2817394733429 | 109.1809153556824 | |
14.4720926213928 | 27.1793007850647 | 26.9595265388489 | 31.5272092819214 | 34.4556629657746 | 14.4720926213928 | 177.1793007850647 | 51.9595265388489 | 31.5272092819214 | 109.4556629657746 | |
14.4792926674731 | 27.2129714488983 | 26.9565999507904 | 31.7697465419769 | 34.6890151500702 | 14.4792926674731 | 177.21297144889832 | 51.956599950790405 | 31.7697465419769 | 109.68901515007019 | |
14.4864927135534 | 27.2475719451905 | 26.952075958252 | 32.0029020309448 | 34.8742067813874 | 14.4864927135534 | 177.2475719451905 | 51.952075958251996 | 32.0029020309448 | 109.8742067813874 | |
14.4936927596337 | 27.2827208042145 | 26.9473671913147 | 32.2202444076538 | 35.0054204463959 | 14.4936927596337 | 177.2827208042145 | 51.9473671913147 | 32.2202444076538 | 110.0054204463959 | |
14.500892805714 | 27.3179471492768 | 26.9435346126557 | 32.4152648448944 | 35.0778937339783 | 14.500892805714 | 177.3179471492768 | 51.9435346126557 | 32.4152648448944 | 110.0778937339783 | |
14.5080928517943 | 27.3527383804321 | 26.9412577152252 | 32.5815558433533 | 35.0879669189453 | 14.5080928517943 | 177.3527383804321 | 51.9412577152252 | 32.5815558433533 | 110.0879669189453 | |
14.5152928978746 | 27.3865759372711 | 26.9409000873566 | 32.7132403850556 | 35.033667087555 | 14.5152928978746 | 177.3865759372711 | 51.940900087356596 | 32.7132403850556 | 110.033667087555 | |
14.5224929439549 | 27.4189889431 | 26.9426226615906 | 32.8056633472443 | 34.9152624607086 | 14.5224929439549 | 177.4189889431 | 51.942622661590605 | 32.8056633472443 | 109.91526246070859 | |
14.5296929900352 | 27.4495363235474 | 26.9462764263153 | 32.8556537628174 | 34.7355961799622 | 14.5296929900352 | 177.4495363235474 | 51.9462764263153 | 32.8556537628174 | 109.7355961799622 | |
14.5368930361155 | 27.4777173995972 | 26.9514203071594 | 32.8614711761475 | 34.4999194145203 | 14.5368930361155 | 177.4777173995972 | 51.951420307159395 | 32.8614711761475 | 109.49991941452029 | |
14.5440930821957 | 27.5029599666596 | 26.9573330879212 | 32.8226566314697 | 34.2155456542969 | 14.5440930821957 | 177.5029599666596 | 51.9573330879212 | 32.8226566314697 | 109.2155456542969 | |
14.551293128276 | 27.5246262550354 | 26.9633173942566 | 32.7401578426361 | 33.8913857936859 | 14.551293128276 | 177.5246262550354 | 51.9633173942566 | 32.7401578426361 | 108.8913857936859 | |
14.5584931743563 | 27.5420606136322 | 26.9688487052918 | 32.6165616512299 | 33.5376024246216 | 14.5584931743563 | 177.5420606136322 | 51.968848705291805 | 32.6165616512299 | 108.53760242462161 | |
14.5656932204366 | 27.5544703006745 | 26.9736647605896 | 32.455986738205 | 33.1650853157044 | 14.5656932204366 | 177.5544703006745 | 51.9736647605896 | 32.455986738205 | 108.1650853157044 | |
14.5728932665169 | 27.5608479976654 | 26.9777536392212 | 32.2638034820557 | 32.7844977378845 | 14.5728932665169 | 177.5608479976654 | 51.9777536392212 | 32.2638034820557 | 107.7844977378845 | |
14.5800933125972 | 27.560156583786 | 26.9811511039734 | 32.0461690425873 | 32.4054718017578 | 14.5800933125972 | 177.560156583786 | 51.9811511039734 | 32.0461690425873 | 107.4054718017578 | |
14.5872933586775 | 27.5516867637634 | 26.9838750362396 | 31.8096160888672 | 32.0360064506531 | 14.5872933586775 | 177.5516867637634 | 51.983875036239596 | 31.8096160888672 | 107.0360064506531 | |
14.5944934047578 | 27.535480260849 | 26.9857883453369 | 31.5608263015747 | 31.6823244094849 | 14.5944934047578 | 177.535480260849 | 51.9857883453369 | 31.5608263015747 | 106.68232440948489 | |
14.6016934508381 | 27.5123357772827 | 26.9866704940796 | 31.3063383102417 | 31.349128484726 | 14.6016934508381 | 177.51233577728271 | 51.986670494079604 | 31.3063383102417 | 106.349128484726 | |
14.6088934969184 | 27.4834454059601 | 26.9860565662384 | 31.0521841049195 | 31.0398638248444 | 14.6088934969184 | 177.4834454059601 | 51.9860565662384 | 31.0521841049195 | 106.0398638248444 | |
14.6160935429987 | 27.4500906467438 | 26.9833385944367 | 30.8035492897034 | 30.756825208664 | 14.6160935429987 | 177.4500906467438 | 51.9833385944367 | 30.8035492897034 | 105.756825208664 | |
14.623293589079 | 27.4136245250702 | 26.9779145717621 | 30.5645406246186 | 30.5013060569763 | 14.623293589079 | 177.4136245250702 | 51.9779145717621 | 30.5645406246186 | 105.5013060569763 | |
14.6304936351593 | 27.3754119873047 | 26.9693970680237 | 30.3381145000458 | 30.2737534046173 | 14.6304936351593 | 177.3754119873047 | 51.969397068023696 | 30.3381145000458 | 105.2737534046173 | |
14.6376936812396 | 27.3367762565613 | 26.9578993320465 | 30.1263570785523 | 30.0740122795105 | 14.6376936812396 | 177.3367762565613 | 51.9578993320465 | 30.1263570785523 | 105.0740122795105 | |
14.6448937273199 | 27.2987246513367 | 26.9439935684204 | 29.9307823181153 | 29.9015998840332 | 14.6448937273199 | 177.2987246513367 | 51.943993568420396 | 29.9307823181153 | 104.9015998840332 | |
14.6520937734002 | 27.2618353366852 | 26.9285798072815 | 29.7524869441986 | 29.7557294368744 | 14.6520937734002 | 177.2618353366852 | 51.9285798072815 | 29.7524869441986 | 104.7557294368744 | |
14.6592938194805 | 27.2264182567597 | 26.912784576416 | 29.5921862125397 | 29.6350836753845 | 14.6592938194805 | 177.2264182567597 | 51.912784576416 | 29.5921862125397 | 104.6350836753845 | |
14.6664938655608 | 27.192747592926 | 26.8980264663696 | 29.4501602649689 | 29.537707567215 | 14.6664938655608 | 177.192747592926 | 51.8980264663696 | 29.4501602649689 | 104.537707567215 | |
14.6736939116411 | 27.1610856056214 | 26.8858671188355 | 29.3263137340546 | 29.4609844684601 | 14.6736939116411 | 177.1610856056214 | 51.8858671188355 | 29.3263137340546 | 104.4609844684601 | |
14.6808939577213 | 27.1317780017853 | 26.8776535987854 | 29.220324754715 | 29.4020056724549 | 14.6808939577213 | 177.1317780017853 | 51.8776535987854 | 29.220324754715 | 104.4020056724549 | |
14.6880940038016 | 27.1051347255707 | 26.8739640712738 | 29.1316390037537 | 29.3579757213593 | 14.6880940038016 | 177.1051347255707 | 51.873964071273804 | 29.1316390037537 | 104.3579757213593 | |
14.6952940498819 | 27.0815253257752 | 26.8743455410004 | 29.0593266487122 | 29.3263614177704 | 14.6952940498819 | 177.0815253257752 | 51.8743455410004 | 29.0593266487122 | 104.3263614177704 | |
14.7024940959622 | 27.0612418651581 | 26.8775463104248 | 29.0019929409027 | 29.3048024177551 | 14.7024940959622 | 177.0612418651581 | 51.877546310424805 | 29.0019929409027 | 104.3048024177551 | |
14.7096941420425 | 27.0444035530091 | 26.8820464611054 | 28.9579391479492 | 29.2909741401673 | 14.7096941420425 | 177.0444035530091 | 51.8820464611054 | 28.9579391479492 | 104.2909741401673 | |
14.7168941881228 | 27.0308315753937 | 26.8864393234253 | 28.9254367351532 | 29.2827308177948 | 14.7168941881228 | 177.0308315753937 | 51.8864393234253 | 28.9254367351532 | 104.2827308177948 | |
14.7240942342031 | 27.0201325416565 | 26.8894970417023 | 28.9028406143189 | 29.2783081531525 | 14.7240942342031 | 177.0201325416565 | 51.8894970417023 | 28.9028406143189 | 104.2783081531525 | |
14.7312942802834 | 27.011787891388 | 26.8901288509369 | 28.8886189460755 | 29.2764246463776 | 14.7312942802834 | 177.011787891388 | 51.890128850936904 | 28.8886189460755 | 104.27642464637759 | |
14.7384943263637 | 27.0051777362824 | 26.8875896930695 | 28.8812577724457 | 29.2762041091919 | 14.7384943263637 | 177.0051777362824 | 51.8875896930695 | 28.8812577724457 | 104.2762041091919 | |
14.745694372444 | 26.9995391368866 | 26.8818736076355 | 28.8792729377747 | 29.2769134044647 | 14.745694372444 | 176.9995391368866 | 51.8818736076355 | 28.8792729377747 | 104.2769134044647 | |
14.7528944185243 | 26.9940495491028 | 26.8737614154816 | 28.8813412189484 | 29.2780637741089 | 14.7528944185243 | 176.9940495491028 | 51.873761415481596 | 28.8813412189484 | 104.2780637741089 | |
14.7600944646046 | 26.9880294799805 | 26.8645226955414 | 28.8862645626068 | 29.2796492576599 | 14.7600944646046 | 176.9880294799805 | 51.864522695541396 | 28.8862645626068 | 104.2796492576599 | |
14.7672945106849 | 26.9811689853668 | 26.8553018569946 | 28.8930118083954 | 29.2823731899262 | 14.7672945106849 | 176.9811689853668 | 51.8553018569946 | 28.8930118083954 | 104.2823731899262 | |
14.7744945567652 | 26.9735455513001 | 26.8468916416168 | 28.9006590843201 | 29.2875409126282 | 14.7744945567652 | 176.9735455513001 | 51.8468916416168 | 28.9006590843201 | 104.2875409126282 | |
14.7816946028455 | 26.9656419754029 | 26.8398702144623 | 28.9084851741791 | 29.2967021465302 | 14.7816946028455 | 176.9656419754029 | 51.8398702144623 | 28.9084851741791 | 104.29670214653021 | |
14.7888946489258 | 26.958304643631 | 26.8348753452301 | 28.9160132408142 | 29.3112456798554 | 14.7888946489258 | 176.958304643631 | 51.8348753452301 | 28.9160132408142 | 104.3112456798554 | |
14.7960946950061 | 26.9526600837708 | 26.8325507640839 | 28.9230644702912 | 29.3322026729584 | 14.7960946950061 | 176.9526600837708 | 51.832550764083905 | 28.9230644702912 | 104.3322026729584 | |
14.8032947410864 | 26.9498825073242 | 26.8333613872528 | 28.92986536026 | 29.3601930141449 | 14.8032947410864 | 176.9498825073242 | 51.8333613872528 | 28.92986536026 | 104.3601930141449 | |
14.8104947871667 | 26.9508123397827 | 26.8374383449555 | 28.9371013641358 | 29.395455121994 | 14.8104947871667 | 176.95081233978271 | 51.8374383449555 | 28.9371013641358 | 104.395455121994 | |
14.817694833247 | 26.9557297229767 | 26.8446266651154 | 28.9459347724915 | 29.4377386569977 | 14.817694833247 | 176.95572972297668 | 51.8446266651154 | 28.9459347724915 | 104.4377386569977 | |
14.8248948793272 | 26.9644498825073 | 26.85467004776 | 28.9577841758728 | 29.486232995987 | 14.8248948793272 | 176.9644498825073 | 51.854670047759996 | 28.9577841758728 | 104.486232995987 | |
14.8320949254075 | 26.9764721393585 | 26.8671333789826 | 28.9740264415741 | 29.5397520065308 | 14.8320949254075 | 176.9764721393585 | 51.8671333789826 | 28.9740264415741 | 104.5397520065308 | |
14.8392949714878 | 26.9911825656891 | 26.8813610076905 | 28.9958834648133 | 29.5970261096955 | 14.8392949714878 | 176.9911825656891 | 51.8813610076905 | 28.9958834648133 | 104.59702610969549 | |
14.8464950175681 | 27.0079493522644 | 26.8965065479279 | 29.0243208408356 | 29.6570718288422 | 14.8464950175681 | 177.0079493522644 | 51.8965065479279 | 29.0243208408356 | 104.65707182884219 | |
14.8536950636484 | 27.0262777805329 | 26.9118070602417 | 29.0599048137665 | 29.7192931175232 | 14.8536950636484 | 177.0262777805329 | 51.9118070602417 | 29.0599048137665 | 104.7192931175232 | |
14.8608951097287 | 27.0459949970246 | 26.9268035888672 | 29.102635383606 | 29.7833442687988 | 14.8608951097287 | 177.0459949970246 | 51.9268035888672 | 29.102635383606 | 104.7833442687988 | |
14.868095155809 | 27.0674288272858 | 26.9413828849793 | 29.1517496109009 | 29.8489153385163 | 14.868095155809 | 177.0674288272858 | 51.941382884979305 | 29.1517496109009 | 104.84891533851629 | |
14.8752952018893 | 27.0910859107971 | 26.9557595252991 | 29.2059481143952 | 29.9156486988068 | 14.8752952018893 | 177.0910859107971 | 51.9557595252991 | 29.2059481143952 | 104.9156486988068 | |
14.8824952479696 | 27.1172463893891 | 26.9703686237335 | 29.26384806633 | 29.9830198287964 | 14.8824952479696 | 177.1172463893891 | 51.9703686237335 | 29.26384806633 | 104.9830198287964 | |
14.8896952940499 | 27.1455645561218 | 26.9858419895172 | 29.3242275714874 | 30.0503432750702 | 14.8896952940499 | 177.1455645561218 | 51.9858419895172 | 29.3242275714874 | 105.0503432750702 | |
14.8968953401302 | 27.175110578537 | 27.0028233528137 | 29.3861508369446 | 30.1166236400604 | 14.8968953401302 | 177.175110578537 | 52.0028233528137 | 29.3861508369446 | 105.1166236400604 | |
14.9040953862105 | 27.2046864032746 | 27.0216107368469 | 29.4488906860352 | 30.1804482936859 | 14.9040953862105 | 177.2046864032746 | 52.021610736846895 | 29.4488906860352 | 105.1804482936859 | |
14.9112954322908 | 27.2330701351166 | 27.0419418811798 | 29.5119524002075 | 30.2400350570679 | 14.9112954322908 | 177.2330701351166 | 52.041941881179795 | 29.5119524002075 | 105.2400350570679 | |
14.9184954783711 | 27.2592186927796 | 27.0629107952118 | 29.5751392841339 | 30.293345451355 | 14.9184954783711 | 177.2592186927796 | 52.0629107952118 | 29.5751392841339 | 105.293345451355 | |
14.9256955244514 | 27.2823572158814 | 27.0832359790802 | 29.6384871006012 | 30.3384125232697 | 14.9256955244514 | 177.2823572158814 | 52.0832359790802 | 29.6384871006012 | 105.3384125232697 | |
14.9328955705317 | 27.3022592067719 | 27.1016716957092 | 29.701840877533 | 30.3735911846161 | 14.9328955705317 | 177.3022592067719 | 52.1016716957092 | 29.701840877533 | 105.3735911846161 | |
14.940095616612 | 27.3194491863251 | 27.1173059940338 | 29.7645568847656 | 30.3977608680725 | 14.940095616612 | 177.3194491863251 | 52.1173059940338 | 29.7645568847656 | 105.3977608680725 | |
14.9472956626923 | 27.3350238800049 | 27.1298110485077 | 29.8253238201142 | 30.4104685783386 | 14.9472956626923 | 177.3350238800049 | 52.129811048507705 | 29.8253238201142 | 105.4104685783386 | |
14.9544957087726 | 27.3500382900238 | 27.1394371986389 | 29.8823118209839 | 30.4119646549225 | 14.9544957087726 | 177.3500382900238 | 52.1394371986389 | 29.8823118209839 | 105.4119646549225 | |
14.9616957548529 | 27.3649036884308 | 27.1468818187714 | 29.9336194992066 | 30.4030478000641 | 14.9616957548529 | 177.3649036884308 | 52.146881818771405 | 29.9336194992066 | 105.4030478000641 | |
14.9688958009331 | 27.3793160915375 | 27.1531939506531 | 29.9775302410126 | 30.3848922252655 | 14.9688958009331 | 177.3793160915375 | 52.153193950653105 | 29.9775302410126 | 105.3848922252655 | |
14.9760958470134 | 27.3925721645355 | 27.159595489502 | 30.0127327442169 | 30.3587973117829 | 14.9760958470134 | 177.3925721645355 | 52.159595489501996 | 30.0127327442169 | 105.3587973117829 | |
14.9832958930937 | 27.4040162563324 | 27.1672546863556 | 30.0383687019348 | 30.3259670734406 | 14.9832958930937 | 177.4040162563324 | 52.167254686355605 | 30.0383687019348 | 105.32596707344061 | |
14.990495939174 | 27.4130463600159 | 27.1768212318421 | 30.0540924072266 | 30.2874684333801 | 14.990495939174 | 177.4130463600159 | 52.1768212318421 | 30.0540924072266 | 105.2874684333801 | |
14.9976959852543 | 27.4189174175263 | 27.1880567073822 | 30.059951543808 | 30.2441000938416 | 14.9976959852543 | 177.4189174175263 | 52.1880567073822 | 30.059951543808 | 105.2441000938416 | |
15.0048960313346 | 27.4208307266236 | 27.1998584270478 | 30.0564646720886 | 30.1964044570923 | 15.0048960313346 | 177.4208307266236 | 52.1998584270478 | 30.0564646720886 | 105.1964044570923 | |
15.0120960774149 | 27.4183988571167 | 27.2106885910034 | 30.0444006919861 | 30.14475107193 | 15.0120960774149 | 177.4183988571167 | 52.210688591003404 | 30.0444006919861 | 105.14475107193 | |
15.0192961234952 | 27.4119913578034 | 27.2190809249878 | 30.0246238708496 | 30.0895154476166 | 15.0192961234952 | 177.4119913578034 | 52.2190809249878 | 30.0246238708496 | 105.0895154476166 | |
15.0264961695755 | 27.4026215076447 | 27.2239804267884 | 29.9979090690613 | 30.0312280654907 | 15.0264961695755 | 177.4026215076447 | 52.2239804267884 | 29.9979090690613 | 105.0312280654907 | |
15.0336962156558 | 27.3912310600281 | 27.2247910499573 | 29.9648761749268 | 29.9706220626831 | 15.0336962156558 | 177.3912310600281 | 52.224791049957304 | 29.9648761749268 | 104.9706220626831 | |
15.0408962617361 | 27.3781001567841 | 27.2215306758881 | 29.9261450767517 | 29.9085319042206 | 15.0408962617361 | 177.37810015678411 | 52.221530675888104 | 29.9261450767517 | 104.9085319042206 | |
15.0480963078164 | 27.3628890514374 | 27.2148132324219 | 29.8825204372406 | 29.8459172248841 | 15.0480963078164 | 177.3628890514374 | 52.2148132324219 | 29.8825204372406 | 104.8459172248841 | |
15.0552963538967 | 27.3452341556549 | 27.2056698799134 | 29.8349499702454 | 29.7838211059571 | 15.0552963538967 | 177.3452341556549 | 52.2056698799134 | 29.8349499702454 | 104.7838211059571 | |
15.062496399977 | 27.3252487182617 | 27.195155620575 | 29.7844529151917 | 29.7233641147614 | 15.062496399977 | 177.3252487182617 | 52.195155620575 | 29.7844529151917 | 104.7233641147614 | |
15.0696964460573 | 27.3034691810608 | 27.1839380264282 | 29.7319710254669 | 29.6655833721161 | 15.0696964460573 | 177.3034691810608 | 52.1839380264282 | 29.7319710254669 | 104.6655833721161 | |
15.0768964921376 | 27.2804975509644 | 27.1721243858338 | 29.6782612800598 | 29.611337184906 | 15.0768964921376 | 177.2804975509644 | 52.1721243858338 | 29.6782612800598 | 104.611337184906 | |
15.0840965382179 | 27.256727218628 | 27.159458398819 | 29.6238362789154 | 29.5612871646881 | 15.0840965382179 | 177.256727218628 | 52.159458398819 | 29.6238362789154 | 104.5612871646881 | |
15.0912965842982 | 27.2325515747071 | 27.145379781723 | 29.5689821243286 | 29.5159459114075 | 15.0912965842982 | 177.2325515747071 | 52.145379781723 | 29.5689821243286 | 104.5159459114075 | |
15.0984966303785 | 27.2086381912232 | 27.1291971206665 | 29.5137226581574 | 29.4757068157196 | 15.0984966303785 | 177.2086381912232 | 52.129197120666504 | 29.5137226581574 | 104.4757068157196 |