Selected Cell
Cell:
Value:
Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
A
B
C
D
E
F
G
H
I
J
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
A
B
C
D
E
F
G
H
I
J
K
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
A
B
C
D
E
F
G
H
I
J
K
L
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
A
B
C
D
E
F
G
H
I
J
K
1901
1902
1903
1904
1905
1906
1907
1908
1909
1910
1911
1912
1913
1914
1915
1916
1917
1918
1919
1920
1921
1922
1923
1924
1925
1926
1927
1928
1929
1930
1931
1932
1933
1934
1935
1936
1937
1938
1939
1940
1941
1942
1943
1944
1945
1946
1947
1948
1949
1950
1951
1952
1953
1954
1955
1956
1957
1958
1959
1960
1961
1962
1963
1964
1965
1966
1967
1968
1969
1970
1971
1972
1973
1974
1975
1976
1977
1978
1979
1980
1981
1982
1983
1984
1985
1986
1987
1988
1989
1990
1991
1992
1993
1994
1995
1996
1997
1998
1999
2000
13.6656874603998 | 27.0044207572937 | 27.2353172302246 | 31.543630361557 | 30.2642524242401 | 13.6656874603998 | 177.0044207572937 | 52.235317230224595 | 31.543630361557 | 105.2642524242401 | |
13.6728875064801 | 26.9942224025727 | 27.2237122058869 | 31.2615334987641 | 30.1250159740448 | 13.6728875064801 | 176.9942224025727 | 52.2237122058869 | 31.2615334987641 | 105.1250159740448 | |
13.6800875525604 | 26.9838988780976 | 27.208936214447 | 30.9955716133118 | 29.9932777881623 | 13.6800875525604 | 176.9838988780976 | 52.208936214447 | 30.9955716133118 | 104.9932777881623 | |
13.6872875986407 | 26.9737899303436 | 27.1926462650299 | 30.7476401329041 | 29.8694908618927 | 13.6872875986407 | 176.9737899303436 | 52.1926462650299 | 30.7476401329041 | 104.8694908618927 | |
13.6944876447209 | 26.9644439220429 | 27.1767020225525 | 30.5190324783325 | 29.7542750835419 | 13.6944876447209 | 176.9644439220429 | 52.176702022552504 | 30.5190324783325 | 104.7542750835419 | |
13.7016876908012 | 26.9564628601074 | 27.1626353263855 | 30.3102791309357 | 29.6483814716339 | 13.7016876908012 | 176.9564628601074 | 52.1626353263855 | 30.3102791309357 | 104.6483814716339 | |
13.7088877368815 | 26.9504249095917 | 27.1513402462006 | 30.1211357116699 | 29.5525252819061 | 13.7088877368815 | 176.9504249095917 | 52.151340246200604 | 30.1211357116699 | 104.5525252819061 | |
13.7160877829618 | 26.9465863704682 | 27.1429717540741 | 29.9508512020111 | 29.467123746872 | 13.7160877829618 | 176.9465863704682 | 52.1429717540741 | 29.9508512020111 | 104.467123746872 | |
13.7232878290421 | 26.9447922706604 | 27.1370112895966 | 29.7985851764679 | 29.3922364711762 | 13.7232878290421 | 176.9447922706604 | 52.1370112895966 | 29.7985851764679 | 104.3922364711762 | |
13.7304878751224 | 26.9445717334748 | 27.1323382854462 | 29.6635806560517 | 29.3275475502014 | 13.7304878751224 | 176.9445717334748 | 52.132338285446195 | 29.6635806560517 | 104.3275475502014 | |
13.7376879212027 | 26.9453406333924 | 27.1274387836457 | 29.545134305954 | 29.2724609375 | 13.7376879212027 | 176.9453406333924 | 52.1274387836457 | 29.545134305954 | 104.2724609375 | |
13.744887967283 | 26.9465327262879 | 27.1207690238953 | 29.4424831867218 | 29.2261600494385 | 13.744887967283 | 176.9465327262879 | 52.1207690238953 | 29.4424831867218 | 104.2261600494385 | |
13.7520880133633 | 26.9475638866425 | 27.1113336086273 | 29.3547928333283 | 29.1876494884491 | 13.7520880133633 | 176.9475638866425 | 52.111333608627305 | 29.3547928333283 | 104.1876494884491 | |
13.7592880594436 | 26.9477844238281 | 27.099084854126 | 29.2811930179596 | 29.1560053825379 | 13.7592880594436 | 176.9477844238281 | 52.099084854126005 | 29.2811930179596 | 104.1560053825379 | |
13.7664881055239 | 26.9466578960419 | 27.0848393440247 | 29.2206227779389 | 29.1304767131806 | 13.7664881055239 | 176.9466578960419 | 52.0848393440247 | 29.2206227779389 | 104.1304767131806 | |
13.7736881516042 | 26.9440114498139 | 27.0698845386505 | 29.1716456413269 | 29.1107296943665 | 13.7736881516042 | 176.9440114498139 | 52.0698845386505 | 29.1716456413269 | 104.1107296943665 | |
13.7808881976845 | 26.9399762153626 | 27.0553946495056 | 29.1324496269226 | 29.0968120098114 | 13.7808881976845 | 176.9399762153626 | 52.0553946495056 | 29.1324496269226 | 104.0968120098114 | |
13.7880882437648 | 26.9347548484802 | 27.0421028137207 | 29.101037979126 | 29.0889143943787 | 13.7880882437648 | 176.9347548484802 | 52.0421028137207 | 29.101037979126 | 104.0889143943787 | |
13.7952882898451 | 26.92831158638 | 27.0304262638092 | 29.0754020214081 | 29.0870904922486 | 13.7952882898451 | 176.92831158638 | 52.030426263809204 | 29.0754020214081 | 104.08709049224859 | |
13.8024883359254 | 26.92049741745 | 27.0205914974213 | 29.0537595748902 | 29.0909886360169 | 13.8024883359254 | 176.92049741745 | 52.0205914974213 | 29.0537595748902 | 104.0909886360169 | |
13.8096883820057 | 26.9113481044769 | 27.0125985145569 | 29.0346145629883 | 29.0997982025147 | 13.8096883820057 | 176.9113481044769 | 52.0125985145569 | 29.0346145629883 | 104.0997982025147 | |
13.816888428086 | 26.9012331962586 | 27.0061075687409 | 29.0169358253479 | 29.1124045848847 | 13.816888428086 | 176.9012331962586 | 52.0061075687409 | 29.0169358253479 | 104.1124045848847 | |
13.8240884741663 | 26.8906533718109 | 27.0005464553833 | 29.0002048015595 | 29.127585887909 | 13.8240884741663 | 176.8906533718109 | 52.0005464553833 | 29.0002048015595 | 104.12758588790899 | |
13.8312885202466 | 26.8799781799317 | 26.995450258255 | 28.9844155311585 | 29.1441917419434 | 13.8312885202466 | 176.8799781799317 | 51.995450258255005 | 28.9844155311585 | 104.1441917419434 | |
13.8384885663268 | 26.8694400787354 | 26.9906103610993 | 28.9699792861939 | 29.1613161563874 | 13.8384885663268 | 176.8694400787354 | 51.9906103610993 | 28.9699792861939 | 104.1613161563874 | |
13.8456886124071 | 26.8593192100525 | 26.9859492778778 | 28.9574682712555 | 29.178661108017 | 13.8456886124071 | 176.8593192100525 | 51.9859492778778 | 28.9574682712555 | 104.178661108017 | |
13.8528886584874 | 26.8501460552216 | 26.9811093807221 | 28.9473950862885 | 29.1967630386353 | 13.8528886584874 | 176.85014605522161 | 51.9811093807221 | 28.9473950862885 | 104.1967630386353 | |
13.8600887045677 | 26.8425464630127 | 26.9753992557526 | 28.9400219917298 | 29.2169809341431 | 13.8600887045677 | 176.8425464630127 | 51.9753992557526 | 28.9400219917298 | 104.2169809341431 | |
13.867288750648 | 26.8370270729065 | 26.9680976867676 | 28.9354324340821 | 29.2410850524903 | 13.867288750648 | 176.8370270729065 | 51.9680976867676 | 28.9354324340821 | 104.24108505249029 | |
13.8744887967283 | 26.8337726593018 | 26.9588768482208 | 28.9337277412415 | 29.2707741260529 | 13.8744887967283 | 176.83377265930181 | 51.9588768482208 | 28.9337277412415 | 104.2707741260529 | |
13.8816888428086 | 26.8326818943024 | 26.9480228424072 | 28.9352238178253 | 29.3073296546936 | 13.8816888428086 | 176.8326818943024 | 51.9480228424072 | 28.9352238178253 | 104.3073296546936 | |
13.8888888888889 | 26.8334627151489 | 26.9363522529602 | 28.9403676986695 | 29.3513059616089 | 13.8888888888889 | 176.8334627151489 | 51.936352252960205 | 28.9403676986695 | 104.3513059616089 | |
13.8960889349692 | 26.8356740474701 | 26.9251167774201 | 28.9495408535004 | 29.4024527072907 | 13.8960889349692 | 176.8356740474701 | 51.9251167774201 | 28.9495408535004 | 104.4024527072907 | |
13.9032889810495 | 26.8386960029602 | 26.915717124939 | 28.9629340171814 | 29.4595241546631 | 13.9032889810495 | 176.8386960029602 | 51.915717124939 | 28.9629340171814 | 104.4595241546631 | |
13.9104890271298 | 26.8418312072754 | 26.9094288349152 | 28.9806962013245 | 29.5205056667328 | 13.9104890271298 | 176.8418312072754 | 51.909428834915204 | 28.9806962013245 | 104.5205056667328 | |
13.9176890732101 | 26.8443048000336 | 26.9070327281952 | 29.0030419826508 | 29.5829713344574 | 13.9176890732101 | 176.8443048000336 | 51.907032728195205 | 29.0030419826508 | 104.5829713344574 | |
13.9248891192904 | 26.845532655716 | 26.9084513187409 | 29.0301382541657 | 29.6445548534394 | 13.9248891192904 | 176.845532655716 | 51.9084513187409 | 29.0301382541657 | 104.6445548534394 | |
13.9320891653707 | 26.8452763557434 | 26.9127726554871 | 29.061758518219 | 29.7031462192536 | 13.9320891653707 | 176.8452763557434 | 51.9127726554871 | 29.061758518219 | 104.7031462192536 | |
13.939289211451 | 26.843786239624 | 26.9184768199921 | 29.0971279144287 | 29.7569751739502 | 13.939289211451 | 176.843786239624 | 51.9184768199921 | 29.0971279144287 | 104.7569751739502 | |
13.9464892575313 | 26.8417656421662 | 26.9238948822022 | 29.1350960731507 | 29.8045933246613 | 13.9464892575313 | 176.8417656421662 | 51.923894882202205 | 29.1350960731507 | 104.8045933246613 | |
13.9536893036116 | 26.8401145935059 | 26.9275307655335 | 29.1745126247406 | 29.8450708389282 | 13.9536893036116 | 176.8401145935059 | 51.927530765533504 | 29.1745126247406 | 104.8450708389282 | |
13.9608893496919 | 26.8396735191345 | 26.9284069538117 | 29.2144417762757 | 29.8780381679535 | 13.9608893496919 | 176.8396735191345 | 51.9284069538117 | 29.2144417762757 | 104.87803816795349 | |
13.9680893957722 | 26.8409430980683 | 26.9260942935944 | 29.2541265487671 | 29.903507232666 | 13.9680893957722 | 176.8409430980683 | 51.9260942935944 | 29.2541265487671 | 104.903507232666 | |
13.9752894418525 | 26.84406042099 | 26.9207358360291 | 29.2929351329804 | 29.9214124679566 | 13.9752894418525 | 176.84406042099 | 51.920735836029095 | 29.2929351329804 | 104.9214124679566 | |
13.9824894879327 | 26.8487095832825 | 26.9129812717438 | 29.3302059173584 | 29.9314379692078 | 13.9824894879327 | 176.8487095832825 | 51.9129812717438 | 29.3302059173584 | 104.93143796920779 | |
13.989689534013 | 26.8542587757111 | 26.9039571285248 | 29.3652772903443 | 29.9332559108734 | 13.989689534013 | 176.8542587757111 | 51.9039571285248 | 29.3652772903443 | 104.9332559108734 | |
13.9968895800933 | 26.859998703003 | 26.8950641155243 | 29.3974220752716 | 29.9268662929535 | 13.9968895800933 | 176.859998703003 | 51.8950641155243 | 29.3974220752716 | 104.92686629295349 | |
14.0040896261736 | 26.8652439117432 | 26.8876492977143 | 29.4259011745453 | 29.9127221107483 | 14.0040896261736 | 176.8652439117432 | 51.887649297714304 | 29.4259011745453 | 104.9127221107483 | |
14.0112896722539 | 26.8696129322052 | 26.8825352191925 | 29.4500768184662 | 29.8915028572083 | 14.0112896722539 | 176.8696129322052 | 51.882535219192505 | 29.4500768184662 | 104.89150285720831 | |
14.0184897183342 | 26.8730521202088 | 26.8798112869263 | 29.469496011734 | 29.8638582229614 | 14.0184897183342 | 176.8730521202088 | 51.8798112869263 | 29.469496011734 | 104.8638582229614 | |
14.0256897644145 | 26.8756747245789 | 26.8791794776917 | 29.4837474822998 | 29.8305809497833 | 14.0256897644145 | 176.87567472457891 | 51.8791794776917 | 29.4837474822998 | 104.8305809497833 | |
14.0328898104948 | 26.8776714801789 | 26.8802464008332 | 29.4925034046173 | 29.7928750514984 | 14.0328898104948 | 176.8776714801789 | 51.8802464008332 | 29.4925034046173 | 104.7928750514984 | |
14.0400898565751 | 26.8790304660797 | 26.8827140331269 | 29.4955611228943 | 29.7524034976959 | 14.0400898565751 | 176.8790304660797 | 51.8827140331269 | 29.4955611228943 | 104.7524034976959 | |
14.0472899026554 | 26.8794417381287 | 26.8860816955567 | 29.4930160045624 | 29.7107458114624 | 14.0472899026554 | 176.8794417381287 | 51.8860816955567 | 29.4930160045624 | 104.7107458114624 | |
14.0544899487357 | 26.878309249878 | 26.8894851207733 | 29.4853389263153 | 29.6689450740814 | 14.0544899487357 | 176.878309249878 | 51.8894851207733 | 29.4853389263153 | 104.66894507408139 | |
14.061689994816 | 26.8747925758362 | 26.8919050693512 | 29.4731318950653 | 29.6274781227112 | 14.061689994816 | 176.8747925758362 | 51.891905069351196 | 29.4731318950653 | 104.6274781227112 | |
14.0688900408963 | 26.8681466579437 | 26.8927395343781 | 29.4568836688996 | 29.5866191387177 | 14.0688900408963 | 176.8681466579437 | 51.8927395343781 | 29.4568836688996 | 104.58661913871771 | |
14.0760900869766 | 26.8580317497254 | 26.8920361995697 | 29.4368505477905 | 29.5467019081116 | 14.0760900869766 | 176.8580317497254 | 51.8920361995697 | 29.4368505477905 | 104.5467019081116 | |
14.0832901330569 | 26.8448650836945 | 26.8902122974396 | 29.4132947921753 | 29.5079588890076 | 14.0832901330569 | 176.84486508369451 | 51.890212297439604 | 29.4132947921753 | 104.5079588890076 | |
14.0904901791372 | 26.8298506736756 | 26.8875658512116 | 29.386705160141 | 29.4702649116516 | 14.0904901791372 | 176.8298506736756 | 51.887565851211605 | 29.386705160141 | 104.4702649116516 | |
14.0976902252175 | 26.814728975296 | 26.8841147422791 | 29.3580412864685 | 29.4332444667816 | 14.0976902252175 | 176.814728975296 | 51.884114742279095 | 29.3580412864685 | 104.4332444667816 | |
14.1048902712978 | 26.8013238906861 | 26.8799543380738 | 29.3286144733429 | 29.3967306613922 | 14.1048902712978 | 176.8013238906861 | 51.8799543380738 | 29.3286144733429 | 104.3967306613922 | |
14.1120903173781 | 26.7910540103913 | 26.8756091594696 | 29.2996227741242 | 29.3611407279968 | 14.1120903173781 | 176.7910540103913 | 51.875609159469604 | 29.2996227741242 | 104.3611407279968 | |
14.1192903634583 | 26.7845928668976 | 26.8719077110291 | 29.2718470096588 | 29.3272733688355 | 14.1192903634583 | 176.7845928668976 | 51.871907711029095 | 29.2718470096588 | 104.3272733688355 | |
14.1264904095386 | 26.7818033695221 | 26.8694877624512 | 29.2455792427063 | 29.2958736419678 | 14.1264904095386 | 176.7818033695221 | 51.8694877624512 | 29.2455792427063 | 104.2958736419678 | |
14.1336904556189 | 26.7817556858063 | 26.8685758113861 | 29.2207777500153 | 29.2673707008362 | 14.1336904556189 | 176.7817556858063 | 51.8685758113861 | 29.2207777500153 | 104.2673707008362 | |
14.1408905016992 | 26.7830610275269 | 26.8689930438996 | 29.1972994804383 | 29.241943359375 | 14.1408905016992 | 176.7830610275269 | 51.8689930438996 | 29.1972994804383 | 104.241943359375 | |
14.1480905477795 | 26.7841935157776 | 26.8704116344452 | 29.1749119758606 | 29.2198419570923 | 14.1480905477795 | 176.7841935157776 | 51.870411634445205 | 29.1749119758606 | 104.2198419570923 | |
14.1552905938598 | 26.7838895320893 | 26.8723785877228 | 29.153060913086 | 29.2013764381409 | 14.1552905938598 | 176.7838895320893 | 51.8723785877228 | 29.153060913086 | 104.2013764381409 | |
14.1624906399401 | 26.7815232276917 | 26.8740594387055 | 29.1309356689453 | 29.1866004467011 | 14.1624906399401 | 176.7815232276917 | 51.8740594387055 | 29.1309356689453 | 104.1866004467011 | |
14.1696906860204 | 26.7773389816284 | 26.874315738678 | 29.1079461574555 | 29.1750967502594 | 14.1696906860204 | 176.7773389816284 | 51.874315738678 | 29.1079461574555 | 104.1750967502594 | |
14.1768907321007 | 26.7723023891449 | 26.8720984458924 | 29.0841698646546 | 29.1660308837891 | 14.1768907321007 | 176.7723023891449 | 51.872098445892405 | 29.0841698646546 | 104.1660308837891 | |
14.184090778181 | 26.767760515213 | 26.8670499324799 | 29.0605127811432 | 29.158490896225 | 14.184090778181 | 176.767760515213 | 51.8670499324799 | 29.0605127811432 | 104.158490896225 | |
14.1912908242613 | 26.7648279666901 | 26.8596708774567 | 29.0383040904999 | 29.151850938797 | 14.1912908242613 | 176.7648279666901 | 51.8596708774567 | 29.0383040904999 | 104.151850938797 | |
14.1984908703416 | 26.7640292644501 | 26.8510639667511 | 29.0186703205109 | 29.145872592926 | 14.1984908703416 | 176.7640292644501 | 51.8510639667511 | 29.0186703205109 | 104.145872592926 | |
14.2056909164219 | 26.7652332782746 | 26.8424451351166 | 29.0021240711212 | 29.1406452655792 | 14.2056909164219 | 176.7652332782746 | 51.8424451351166 | 29.0021240711212 | 104.1406452655792 | |
14.2128909625022 | 26.7679452896118 | 26.8349826335907 | 28.9886295795441 | 29.1364014148712 | 14.2128909625022 | 176.7679452896118 | 51.8349826335907 | 28.9886295795441 | 104.1364014148712 | |
14.2200910085825 | 26.7714679241181 | 26.8297553062439 | 28.9778649806977 | 29.1334509849549 | 14.2200910085825 | 176.7714679241181 | 51.8297553062439 | 28.9778649806977 | 104.1334509849549 | |
14.2272910546628 | 26.7751276493073 | 26.8276631832123 | 28.9692997932434 | 29.1321277618408 | 14.2272910546628 | 176.7751276493073 | 51.8276631832123 | 28.9692997932434 | 104.13212776184079 | |
14.2344911007431 | 26.7784476280213 | 26.8289625644684 | 28.9622008800507 | 29.1327059268952 | 14.2344911007431 | 176.7784476280213 | 51.8289625644684 | 28.9622008800507 | 104.1327059268952 | |
14.2416911468234 | 26.7813563346863 | 26.8330574035645 | 28.9556562900543 | 29.1353046894074 | 14.2416911468234 | 176.7813563346863 | 51.833057403564496 | 28.9556562900543 | 104.1353046894074 | |
14.2488911929037 | 26.7842948436737 | 26.8386542797089 | 28.9486646652222 | 29.139792919159 | 14.2488911929037 | 176.7842948436737 | 51.838654279708905 | 28.9486646652222 | 104.13979291915899 | |
14.256091238984 | 26.7880380153656 | 26.8444180488587 | 28.9406597614289 | 29.1458070278168 | 14.256091238984 | 176.7880380153656 | 51.8444180488587 | 28.9406597614289 | 104.1458070278168 | |
14.2632912850642 | 26.7932891845703 | 26.8495619297028 | 28.9317667484284 | 29.1529357433319 | 14.2632912850642 | 176.7932891845703 | 51.8495619297028 | 28.9317667484284 | 104.1529357433319 | |
14.2704913311445 | 26.8004179000855 | 26.8538475036621 | 28.9227366447449 | 29.161137342453 | 14.2704913311445 | 176.8004179000855 | 51.853847503662095 | 28.9227366447449 | 104.161137342453 | |
14.2776913772248 | 26.8093705177307 | 26.857191324234 | 28.9144814014435 | 29.170972108841 | 14.2776913772248 | 176.8093705177307 | 51.857191324234 | 28.9144814014435 | 104.170972108841 | |
14.2848914233051 | 26.8197119235993 | 26.8592774868012 | 28.9077341556549 | 29.183691740036 | 14.2848914233051 | 176.8197119235993 | 51.859277486801204 | 28.9077341556549 | 104.183691740036 | |
14.2920914693854 | 26.8305957317353 | 26.8596947193146 | 28.902941942215 | 29.2010545730591 | 14.2920914693854 | 176.8305957317353 | 51.859694719314604 | 28.902941942215 | 104.2010545730591 | |
14.2992915154657 | 26.8408358097077 | 26.8581807613373 | 28.9004981517792 | 29.2251825332642 | 14.2992915154657 | 176.8408358097077 | 51.8581807613373 | 28.9004981517792 | 104.2251825332642 | |
14.306491561546 | 26.8492698669434 | 26.8548309803009 | 28.9009034633637 | 29.2583584785462 | 14.306491561546 | 176.8492698669434 | 51.8548309803009 | 28.9009034633637 | 104.2583584785462 | |
14.3136916076263 | 26.8553256988526 | 26.8499374389649 | 28.9047300815582 | 29.3029427528382 | 14.3136916076263 | 176.8553256988526 | 51.8499374389649 | 28.9047300815582 | 104.30294275283819 | |
14.3208916537066 | 26.8591761589051 | 26.8438160419464 | 28.9125859737396 | 29.3610870838165 | 14.3208916537066 | 176.8591761589051 | 51.8438160419464 | 28.9125859737396 | 104.3610870838165 | |
14.3280916997869 | 26.8614292144776 | 26.8368065357208 | 28.925359249115 | 29.4344604015351 | 14.3280916997869 | 176.8614292144776 | 51.8368065357208 | 28.925359249115 | 104.4344604015351 | |
14.3352917458672 | 26.8626928329468 | 26.8293976783753 | 28.9443612098694 | 29.5241415500641 | 14.3352917458672 | 176.8626928329468 | 51.8293976783753 | 28.9443612098694 | 104.5241415500641 | |
14.3424917919475 | 26.8634200096131 | 26.8223464488983 | 28.9712727069855 | 29.630708694458 | 14.3424917919475 | 176.8634200096131 | 51.8223464488983 | 28.9712727069855 | 104.63070869445801 | |
14.3496918380278 | 26.8641948699951 | 26.816588640213 | 29.0077567100525 | 29.7545433044434 | 14.3496918380278 | 176.8641948699951 | 51.816588640213 | 29.0077567100525 | 104.7545433044434 | |
14.3568918841081 | 26.8657743930817 | 26.8130242824555 | 29.0550410747528 | 29.8961281776428 | 14.3568918841081 | 176.8657743930817 | 51.8130242824555 | 29.0550410747528 | 104.8961281776428 | |
14.3640919301884 | 26.8688619136811 | 26.8122851848603 | 29.1137933731079 | 30.0561666488648 | 14.3640919301884 | 176.8688619136811 | 51.8122851848603 | 29.1137933731079 | 105.0561666488648 | |
14.3712919762687 | 26.8737614154816 | 26.8145859241486 | 29.1843295097351 | 30.23561835289 | 14.3712919762687 | 176.8737614154816 | 51.8145859241486 | 29.1843295097351 | 105.23561835289 | |
14.378492022349 | 26.8804430961609 | 26.8196046352387 | 29.2667746543884 | 30.4357349872589 | 14.378492022349 | 176.8804430961609 | 51.819604635238704 | 29.2667746543884 | 105.4357349872589 |