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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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0.705604515868902 | 29.1469871997833 | 29.8806548118592 | 29.1635751724243 | 29.2430341243744 | 0.705604515868902 | 179.1469871997833 | 54.8806548118592 | 29.1635751724243 | 104.2430341243744 | |
0.712804561949197 | 29.1557788848877 | 29.8703074455261 | 29.1581094264984 | 29.2419195175171 | 0.712804561949197 | 179.1557788848877 | 54.8703074455261 | 29.1581094264984 | 104.2419195175171 | |
0.720004608029492 | 29.1677713394165 | 29.8577249050141 | 29.1511714458466 | 29.2408764362335 | 0.720004608029492 | 179.1677713394165 | 54.857724905014095 | 29.1511714458466 | 104.24087643623349 | |
0.727204654109787 | 29.1823029518128 | 29.8443734645844 | 29.1439056396485 | 29.239958524704 | 0.727204654109787 | 179.1823029518128 | 54.8443734645844 | 29.1439056396485 | 104.23995852470401 | |
0.734404700190081 | 29.1984260082245 | 29.8320770263672 | 29.1373431682587 | 29.2387068271637 | 0.734404700190081 | 179.1984260082245 | 54.8320770263672 | 29.1373431682587 | 104.2387068271637 | |
0.741604746270376 | 29.2149782180786 | 29.8224985599518 | 29.1324973106384 | 29.2365550994873 | 0.741604746270376 | 179.2149782180786 | 54.822498559951796 | 29.1324973106384 | 104.2365550994873 | |
0.748804792350671 | 29.2308926582337 | 29.8166990280152 | 29.1304171085358 | 29.233056306839 | 0.748804792350671 | 179.2308926582337 | 54.8166990280152 | 29.1304171085358 | 104.233056306839 | |
0.756004838430966 | 29.2452573776245 | 29.815012216568 | 29.1318416595459 | 29.2278230190277 | 0.756004838430966 | 179.2452573776245 | 54.815012216568 | 29.1318416595459 | 104.2278230190277 | |
0.763204884511261 | 29.2572855949402 | 29.817396402359 | 29.1369676589966 | 29.220449924469 | 0.763204884511261 | 179.25728559494019 | 54.817396402359 | 29.1369676589966 | 104.220449924469 | |
0.770404930591556 | 29.2662084102631 | 29.823648929596 | 29.1452348232269 | 29.2106926441193 | 0.770404930591556 | 179.2662084102631 | 54.823648929596004 | 29.1452348232269 | 104.2106926441193 | |
0.777604976671851 | 29.2713940143585 | 29.8332035541535 | 29.1555523872376 | 29.1988015174866 | 0.777604976671851 | 179.2713940143585 | 54.8332035541535 | 29.1555523872376 | 104.1988015174866 | |
0.784805022752146 | 29.2725086212158 | 29.8449158668518 | 29.1666269302368 | 29.1856527328491 | 0.784805022752146 | 179.2725086212158 | 54.8449158668518 | 29.1666269302368 | 104.18565273284909 | |
0.792005068832441 | 29.2697250843048 | 29.8572063446045 | 29.1772425174713 | 29.1726768016815 | 0.792005068832441 | 179.2697250843048 | 54.8572063446045 | 29.1772425174713 | 104.1726768016815 | |
0.799205114912736 | 29.2636632919312 | 29.8685133457184 | 29.1863918304444 | 29.1615724563599 | 0.799205114912736 | 179.2636632919312 | 54.8685133457184 | 29.1863918304444 | 104.16157245635989 | |
0.806405160993031 | 29.255360364914 | 29.877644777298 | 29.193526506424 | 29.1540384292603 | 0.806405160993031 | 179.255360364914 | 54.877644777298 | 29.193526506424 | 104.1540384292603 | |
0.813605207073326 | 29.2461097240448 | 29.8837840557099 | 29.1987597942353 | 29.1515409946442 | 0.813605207073326 | 179.2461097240448 | 54.883784055709896 | 29.1987597942353 | 104.1515409946442 | |
0.82080525315362 | 29.2373895645142 | 29.8863112926483 | 29.2028367519379 | 29.1547775268555 | 0.82080525315362 | 179.2373895645142 | 54.8863112926483 | 29.2028367519379 | 104.1547775268555 | |
0.828005299233915 | 29.2306482791901 | 29.8848569393158 | 29.2068064212799 | 29.1633844375611 | 0.828005299233915 | 179.2306482791901 | 54.884856939315796 | 29.2068064212799 | 104.16338443756109 | |
0.83520534531421 | 29.2269825935364 | 29.8796057701111 | 29.2116403579712 | 29.17600274086 | 0.83520534531421 | 179.2269825935364 | 54.8796057701111 | 29.2116403579712 | 104.17600274086 | |
0.842405391394505 | 29.2268514633179 | 29.8714637756348 | 29.2179465293884 | 29.1906535625458 | 0.842405391394505 | 179.2268514633179 | 54.8714637756348 | 29.2179465293884 | 104.1906535625458 | |
0.8496054374748 | 29.2299568653107 | 29.8618614673615 | 29.2258560657501 | 29.2052805423737 | 0.8496054374748 | 179.2299568653107 | 54.8618614673615 | 29.2258560657501 | 104.2052805423737 | |
0.856805483555095 | 29.2353570461273 | 29.8523724079132 | 29.2350292205811 | 29.2181193828583 | 0.856805483555095 | 179.2353570461273 | 54.8523724079132 | 29.2350292205811 | 104.2181193828583 | |
0.86400552963539 | 29.2417168617249 | 29.8444747924805 | 29.244613647461 | 29.227739572525 | 0.86400552963539 | 179.2417168617249 | 54.8444747924805 | 29.244613647461 | 104.227739572525 | |
0.871205575715685 | 29.247659444809 | 29.8395454883576 | 29.2533695697785 | 29.2331755161286 | 0.871205575715685 | 179.247659444809 | 54.8395454883576 | 29.2533695697785 | 104.2331755161286 | |
0.87840562179598 | 29.2520165443421 | 29.838764667511 | 29.2599260807038 | 29.2341887950897 | 0.87840562179598 | 179.2520165443421 | 54.838764667511 | 29.2599260807038 | 104.2341887950897 | |
0.885605667876275 | 29.2540550231934 | 29.8428475856781 | 29.2632400989533 | 29.2313754558563 | 0.885605667876275 | 179.2540550231934 | 54.8428475856781 | 29.2632400989533 | 104.2313754558563 | |
0.89280571395657 | 29.2535483837128 | 29.8516571521759 | 29.2630910873413 | 29.2259454727173 | 0.89280571395657 | 179.2535483837128 | 54.8516571521759 | 29.2630910873413 | 104.2259454727173 | |
0.900005760036865 | 29.2508065700531 | 29.8641741275788 | 29.2602181434632 | 29.2194187641144 | 0.900005760036865 | 179.2508065700531 | 54.8641741275788 | 29.2602181434632 | 104.21941876411441 | |
0.907205806117159 | 29.2464911937714 | 29.8789203166962 | 29.2560756206513 | 29.2131006717682 | 0.907205806117159 | 179.2464911937714 | 54.878920316696195 | 29.2560756206513 | 104.2131006717682 | |
0.914405852197454 | 29.2416393756867 | 29.8944652080536 | 29.2522370815277 | 29.2079508304596 | 0.914405852197454 | 179.2416393756867 | 54.8944652080536 | 29.2522370815277 | 104.2079508304596 | |
0.921605898277749 | 29.2373597621918 | 29.9096524715424 | 29.2498946189881 | 29.2046189308167 | 0.921605898277749 | 179.2373597621918 | 54.9096524715424 | 29.2498946189881 | 104.20461893081671 | |
0.928805944358044 | 29.2346835136414 | 29.9235343933106 | 29.2497277259827 | 29.203599691391 | 0.928805944358044 | 179.23468351364141 | 54.923534393310604 | 29.2497277259827 | 104.20359969139099 | |
0.936005990438339 | 29.2342960834503 | 29.9354732036591 | 29.2519927024842 | 29.2052567005158 | 0.936005990438339 | 179.2342960834503 | 54.9354732036591 | 29.2519927024842 | 104.2052567005158 | |
0.943206036518634 | 29.2363464832306 | 29.945307970047 | 29.256421327591 | 29.2098760604859 | 0.943206036518634 | 179.2363464832306 | 54.945307970047 | 29.256421327591 | 104.2098760604859 | |
0.950406082598929 | 29.2405664920807 | 29.9535632133484 | 29.2620778083801 | 29.2174935340882 | 0.950406082598929 | 179.2405664920807 | 54.9535632133484 | 29.2620778083801 | 104.21749353408819 | |
0.957606128679224 | 29.246598482132 | 29.9611389636994 | 29.2674362659455 | 29.2277753353119 | 0.957606128679224 | 179.24659848213201 | 54.9611389636994 | 29.2674362659455 | 104.2277753353119 | |
0.964806174759519 | 29.2541325092316 | 29.9686074256897 | 29.2708814144135 | 29.2401731014252 | 0.964806174759519 | 179.2541325092316 | 54.9686074256897 | 29.2708814144135 | 104.2401731014252 | |
0.972006220839814 | 29.262900352478 | 29.9758315086365 | 29.2716026306153 | 29.2540013790131 | 0.972006220839814 | 179.262900352478 | 54.9758315086365 | 29.2716026306153 | 104.2540013790131 | |
0.979206266920109 | 29.2725145816803 | 29.9821615219116 | 29.2699217796326 | 29.2683959007263 | 0.979206266920109 | 179.2725145816803 | 54.9821615219116 | 29.2699217796326 | 104.2683959007263 | |
0.986406313000403 | 29.2824566364289 | 29.9868524074555 | 29.2668759822846 | 29.2820990085602 | 0.986406313000403 | 179.2824566364289 | 54.9868524074555 | 29.2668759822846 | 104.2820990085602 | |
0.993606359080698 | 29.2922914028168 | 29.9894630908966 | 29.263573884964 | 29.2933106422425 | 0.993606359080698 | 179.2922914028168 | 54.9894630908966 | 29.263573884964 | 104.2933106422425 | |
1.000806405161 | 29.3018758296967 | 29.9897730350495 | 29.2608857154846 | 29.300057888031 | 1.000806405161 | 179.3018758296967 | 54.989773035049495 | 29.2608857154846 | 104.300057888031 | |
1.00800645124129 | 29.3110966682434 | 29.9876093864441 | 29.2595446109772 | 29.3009161949158 | 1.00800645124129 | 179.3110966682434 | 54.9876093864441 | 29.2595446109772 | 104.3009161949158 | |
1.01520649732158 | 29.3195724487305 | 29.9829304218292 | 29.2602002620697 | 29.295551776886 | 1.01520649732158 | 179.3195724487305 | 54.982930421829195 | 29.2602002620697 | 104.295551776886 | |
1.02240654340188 | 29.3265998363495 | 29.9761056900025 | 29.2629599571228 | 29.2847275733948 | 1.02240654340188 | 179.3265998363495 | 54.9761056900025 | 29.2629599571228 | 104.2847275733948 | |
1.02960658948217 | 29.3313324451447 | 29.967874288559 | 29.267019033432 | 29.2699694633484 | 1.02960658948217 | 179.3313324451447 | 54.967874288559 | 29.267019033432 | 104.2699694633484 | |
1.03680663556247 | 29.3333172798157 | 29.959100484848 | 29.2709290981293 | 29.2533099651337 | 1.03680663556247 | 179.3333172798157 | 54.959100484848 | 29.2709290981293 | 104.2533099651337 | |
1.04400668164276 | 29.3325483798981 | 29.9504935741425 | 29.273396730423 | 29.2371809482575 | 1.04400668164276 | 179.3325483798981 | 54.9504935741425 | 29.273396730423 | 104.2371809482575 | |
1.05120672772306 | 29.329252243042 | 29.9424648284912 | 29.2739033699036 | 29.2241156101227 | 1.05120672772306 | 179.329252243042 | 54.9424648284912 | 29.2739033699036 | 104.2241156101227 | |
1.05840677380335 | 29.3236970901489 | 29.9353659152985 | 29.2724967002869 | 29.2162299156189 | 1.05840677380335 | 179.3236970901489 | 54.935365915298505 | 29.2724967002869 | 104.2162299156189 | |
1.06560681988365 | 29.3161928653717 | 29.9296140670777 | 29.2694687843323 | 29.2144238948822 | 1.06560681988365 | 179.3161928653717 | 54.9296140670777 | 29.2694687843323 | 104.2144238948822 | |
1.07280686596394 | 29.3072402477265 | 29.9256026744843 | 29.2651116847992 | 29.2181551456452 | 1.07280686596394 | 179.3072402477265 | 54.925602674484296 | 29.2651116847992 | 104.2181551456452 | |
1.08000691204424 | 29.2976200580597 | 29.9236536026001 | 29.2599141597748 | 29.2256951332092 | 1.08000691204424 | 179.2976200580597 | 54.9236536026001 | 29.2599141597748 | 104.2256951332092 | |
1.08720695812453 | 29.2882561683655 | 29.9241065979004 | 29.2546153068543 | 29.2349338531494 | 1.08720695812453 | 179.2882561683655 | 54.924106597900405 | 29.2546153068543 | 104.2349338531494 | |
1.09440700420483 | 29.2799472808838 | 29.9272894859314 | 29.2499482631684 | 29.2441368103028 | 1.09440700420483 | 179.2799472808838 | 54.9272894859314 | 29.2499482631684 | 104.24413681030279 | |
1.10160705028512 | 29.2733073234558 | 29.9333214759827 | 29.2462646961212 | 29.2523264884949 | 1.10160705028512 | 179.2733073234558 | 54.9333214759827 | 29.2462646961212 | 104.2523264884949 | |
1.10880709636542 | 29.2687237262726 | 29.941737651825 | 29.2434930801392 | 29.2592585086823 | 1.10880709636542 | 179.2687237262726 | 54.941737651825 | 29.2434930801392 | 104.25925850868231 | |
1.11600714244571 | 29.2664408683777 | 29.9512267112732 | 29.2414605617523 | 29.2651534080506 | 1.11600714244571 | 179.26644086837769 | 54.9512267112732 | 29.2414605617523 | 104.2651534080506 | |
1.12320718852601 | 29.2665719985962 | 29.9599409103394 | 29.2401254177094 | 29.2703866958618 | 1.12320718852601 | 179.2665719985962 | 54.9599409103394 | 29.2401254177094 | 104.2703866958618 | |
1.1304072346063 | 29.2690098285675 | 29.9661338329315 | 29.2394816875458 | 29.2754173278809 | 1.1304072346063 | 179.2690098285675 | 54.966133832931504 | 29.2394816875458 | 104.2754173278809 | |
1.1376072806866 | 29.2732775211334 | 29.9686253070832 | 29.23943400383 | 29.2806446552277 | 1.1376072806866 | 179.2732775211334 | 54.9686253070832 | 29.23943400383 | 104.2806446552277 | |
1.14480732676689 | 29.278439283371 | 29.9670159816742 | 29.239696264267 | 29.2861461639405 | 1.14480732676689 | 179.278439283371 | 54.9670159816742 | 29.239696264267 | 104.2861461639405 | |
1.15200737284719 | 29.2832732200623 | 29.9616098403931 | 29.2397499084473 | 29.2914032936096 | 1.15200737284719 | 179.2832732200623 | 54.961609840393095 | 29.2397499084473 | 104.2914032936096 | |
1.15920741892748 | 29.28666472435 | 29.9532175064087 | 29.2390704154968 | 29.2953491210938 | 1.15920741892748 | 179.28666472435 | 54.9532175064087 | 29.2390704154968 | 104.2953491210938 | |
1.16640746500778 | 29.2879164218903 | 29.9431443214417 | 29.2371273040772 | 29.2968451976776 | 1.16640746500778 | 179.28791642189032 | 54.9431443214417 | 29.2371273040772 | 104.2968451976776 | |
1.17360751108807 | 29.2868137359619 | 29.9331188201905 | 29.2335987091065 | 29.2952954769135 | 1.17360751108807 | 179.2868137359619 | 54.9331188201905 | 29.2335987091065 | 104.2952954769135 | |
1.18080755716837 | 29.2834341526032 | 29.9248933792114 | 29.228413105011 | 29.2909026145935 | 1.18080755716837 | 179.2834341526032 | 54.9248933792114 | 29.228413105011 | 104.2909026145935 | |
1.18800760324866 | 29.2780101299286 | 29.9198806285858 | 29.2217314243317 | 29.2844831943512 | 1.18800760324866 | 179.2780101299286 | 54.9198806285858 | 29.2217314243317 | 104.2844831943512 | |
1.19520764932896 | 29.2709648609162 | 29.918646812439 | 29.2138576507569 | 29.2770922183991 | 1.19520764932896 | 179.2709648609162 | 54.918646812439 | 29.2138576507569 | 104.2770922183991 | |
1.20240769540925 | 29.2629539966583 | 29.9208343029022 | 29.2052984237671 | 29.2696237564087 | 1.20240769540925 | 179.2629539966583 | 54.9208343029022 | 29.2052984237671 | 104.26962375640869 | |
1.20960774148955 | 29.2547881603241 | 29.9255073070526 | 29.1967213153839 | 29.2626738548279 | 1.20960774148955 | 179.2547881603241 | 54.9255073070526 | 29.1967213153839 | 104.2626738548279 | |
1.21680778756984 | 29.247134923935 | 29.9316167831421 | 29.1889429092407 | 29.2564511299134 | 1.21680778756984 | 179.247134923935 | 54.931616783142104 | 29.1889429092407 | 104.2564511299134 | |
1.22400783365014 | 29.2403757572174 | 29.9381792545319 | 29.1826188564301 | 29.2507827281952 | 1.22400783365014 | 179.2403757572174 | 54.9381792545319 | 29.1826188564301 | 104.2507827281952 | |
1.23120787973043 | 29.2346775531769 | 29.9443423748017 | 29.1779458522797 | 29.2453587055207 | 1.23120787973043 | 179.2346775531769 | 54.9443423748017 | 29.1779458522797 | 104.2453587055207 | |
1.23840792581073 | 29.2302310466767 | 29.9492835998535 | 29.174667596817 | 29.2399406433106 | 1.23840792581073 | 179.2302310466767 | 54.9492835998535 | 29.174667596817 | 104.2399406433106 | |
1.24560797189102 | 29.2273044586182 | 29.9523174762726 | 29.1723787784577 | 29.2344748973847 | 1.24560797189102 | 179.2273044586182 | 54.9523174762726 | 29.1723787784577 | 104.2344748973847 | |
1.25280801797132 | 29.2262136936188 | 29.9531936645508 | 29.1709125041962 | 29.2290449142456 | 1.25280801797132 | 179.2262136936188 | 54.953193664550795 | 29.1709125041962 | 104.2290449142456 | |
1.26000806405161 | 29.227089881897 | 29.9522638320923 | 29.1702926158905 | 29.2238652706146 | 1.26000806405161 | 179.227089881897 | 54.9522638320923 | 29.1702926158905 | 104.2238652706146 | |
1.26720811013191 | 29.229736328125 | 29.9503743648529 | 29.1705012321472 | 29.2192339897156 | 1.26720811013191 | 179.229736328125 | 54.950374364852905 | 29.1705012321472 | 104.2192339897156 | |
1.2744081562122 | 29.233729839325 | 29.9484789371491 | 29.1713178157807 | 29.215407371521 | 1.2744081562122 | 179.233729839325 | 54.948478937149105 | 29.1713178157807 | 104.215407371521 | |
1.2816082022925 | 29.2385280132294 | 29.9473166465759 | 29.1724562644959 | 29.2124330997467 | 1.2816082022925 | 179.2385280132294 | 54.9473166465759 | 29.1724562644959 | 104.2124330997467 | |
1.28880824837279 | 29.2435705661774 | 29.947179555893 | 29.1738748550415 | 29.2100250720978 | 1.28880824837279 | 179.2435705661774 | 54.947179555893 | 29.1738748550415 | 104.2100250720978 | |
1.29600829445309 | 29.248458147049 | 29.9479424953461 | 29.1757941246033 | 29.2077898979187 | 1.29600829445309 | 179.248458147049 | 54.9479424953461 | 29.1757941246033 | 104.2077898979187 | |
1.30320834053338 | 29.2530298233032 | 29.9492478370667 | 29.1783630847931 | 29.2054355144501 | 1.30320834053338 | 179.2530298233032 | 54.9492478370667 | 29.1783630847931 | 104.2054355144501 | |
1.31040838661368 | 29.2573928833008 | 29.9506545066834 | 29.1813790798187 | 29.2030453681946 | 1.31040838661368 | 179.2573928833008 | 54.9506545066834 | 29.1813790798187 | 104.20304536819461 | |
1.31760843269397 | 29.2619645595551 | 29.9516916275025 | 29.1843295097351 | 29.200953245163 | 1.31760843269397 | 179.2619645595551 | 54.9516916275025 | 29.1843295097351 | 104.20095324516299 | |
1.32480847877427 | 29.2672753334046 | 29.9519896507263 | 29.186874628067 | 29.1996419429779 | 1.32480847877427 | 179.2672753334046 | 54.951989650726304 | 29.186874628067 | 104.1996419429779 | |
1.33200852485456 | 29.2737901210785 | 29.9515187740326 | 29.1890859603882 | 29.1995704174042 | 1.33200852485456 | 179.2737901210785 | 54.9515187740326 | 29.1890859603882 | 104.1995704174042 | |
1.33920857093486 | 29.2817175388336 | 29.9507260322571 | 29.191267490387 | 29.2011201381684 | 1.33920857093486 | 179.2817175388336 | 54.9507260322571 | 29.191267490387 | 104.20112013816839 | |
1.34640861701515 | 29.2908549308777 | 29.9506723880768 | 29.1936159133911 | 29.2044639587403 | 1.34640861701515 | 179.29085493087769 | 54.950672388076796 | 29.1936159133911 | 104.20446395874029 | |
1.35360866309545 | 29.3005704879761 | 29.9525737762451 | 29.1960000991821 | 29.2094349861145 | 1.35360866309545 | 179.3005704879761 | 54.9525737762451 | 29.1960000991821 | 104.2094349861145 | |
1.36080870917574 | 29.3099284172058 | 29.9571931362152 | 29.1983127593994 | 29.2156457901001 | 1.36080870917574 | 179.3099284172058 | 54.957193136215196 | 29.1983127593994 | 104.2156457901001 | |
1.36800875525604 | 29.3178379535675 | 29.9644470214844 | 29.2008757591248 | 29.2227685451508 | 1.36800875525604 | 179.3178379535675 | 54.9644470214844 | 29.2008757591248 | 104.2227685451508 | |
1.37520880133633 | 29.3234467506409 | 29.9735605716706 | 29.2044818401337 | 29.2309105396271 | 1.37520880133633 | 179.3234467506409 | 54.9735605716706 | 29.2044818401337 | 104.2309105396271 | |
1.38240884741663 | 29.3262660503388 | 29.9833834171295 | 29.2100787162781 | 29.2406380176544 | 1.38240884741663 | 179.3262660503388 | 54.9833834171295 | 29.2100787162781 | 104.2406380176544 | |
1.38960889349692 | 29.326343536377 | 29.9928069114685 | 29.2184233665467 | 29.2528569698334 | 1.38960889349692 | 179.326343536377 | 54.9928069114685 | 29.2184233665467 | 104.2528569698334 | |
1.39680893957722 | 29.3241202831269 | 30.0008535385132 | 29.2300820350647 | 29.2686223983765 | 1.39680893957722 | 179.3241202831269 | 55.0008535385132 | 29.2300820350647 | 104.2686223983765 | |
1.40400898565751 | 29.3202519416809 | 30.0066351890564 | 29.2457938194275 | 29.2890310287476 | 1.40400898565751 | 179.3202519416809 | 55.0066351890564 | 29.2457938194275 | 104.2890310287476 | |
1.41120903173781 | 29.3154358863831 | 30.0095736980438 | 29.2667865753174 | 29.3152809143067 | 1.41120903173781 | 179.3154358863831 | 55.009573698043795 | 29.2667865753174 | 104.3152809143067 | |
1.4184090778181 | 29.3103277683258 | 30.0096988677979 | 29.2947173118592 | 29.3486654758454 | 1.4184090778181 | 179.3103277683258 | 55.0096988677979 | 29.2947173118592 | 104.3486654758454 |